CN106478266A - A kind of disease preventing type cultivation matrix and its preparation method and application - Google Patents
A kind of disease preventing type cultivation matrix and its preparation method and application Download PDFInfo
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- CN106478266A CN106478266A CN201610918075.7A CN201610918075A CN106478266A CN 106478266 A CN106478266 A CN 106478266A CN 201610918075 A CN201610918075 A CN 201610918075A CN 106478266 A CN106478266 A CN 106478266A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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Abstract
The present invention relates to field of plant growing technology is and in particular to a kind of disease preventing type cultivation matrix and its preparation method and application, described disease preventing type cultivation matrix is made up of the raw material of following weight ratio：Compost 20 40%, peat 50 70% and perlite 5 15%, compost is made up of compost and composite bacteria agent capable, and composite bacteria agent capable accounts for the weight of compost than for 0.1 0.5%；Compost is mainly made up of following raw material：Agriculture and forestry organic waste material, water, nitrogen source and phosphorus source, water accounts for the weight of compost than for 50 60%；Composite bacteria agent capable is mainly made by with hypothallus：Colloid bacillus cereus, bacillus subtilises, bacillus megaterium, Trichoderma viride, saccharomyces cerevisiae, candida mycoderma and proteolysis candida mycoderma.The disease preventing type cultivation matrix being obtained using this preparation method, is not only had the advantages that renewable, with low cost and environmental protection, and is applied in cultivated tomato, can preferably play the effect preventing and treating bacterial wilt, improves the survival rate of tomato seedling.
Description
Technical field
The present invention relates to field of plant growing technology and in particular to a kind of disease preventing type cultivation matrix and preparation method thereof and should
With.
Background technology
During tomato planting, nursery is important step.In prior art, using hole plate seedling growth, it has energy-conservation, province
Goods, materials and equipments, save labor and be greatly enhanced seeling industry efficiency, seedling quality is good, the advantages of be suitable to long-distance transportation.Hole tray is educated
Miao Zhong, selects suitable cultivation matrix to be core and key.
The cultivation matrix being usually used in Fructus Lycopersici esculenti is mainly turf, lacks below the cultivation matrix presence with turf as primary raw material
Point：First, turf originates in China northeast, by regional limitation and be non-renewable resources, on the one hand, lead to turf relatively costly,
And then lead to the relatively costly of the cultivation matrix with turf as primary raw material, and on the other hand, such as exhaustive exploitation and use turf, must
Turf resource exhaustion so can be caused；Second, the exploitation processing of turf can cause very big destruction and pollution to environment, thus destroying
Ecological balance；3rd, turf effectively cannot be prevented to the bacterial wilt of Fructus Lycopersici esculenti, bacterial wilt be one kind be distributed widely in the torrid zone,
Subtropical zones and the worldwide disease of some Temperate Region in Chinas, are the principal diseases causing the Fructus Lycopersici esculenti underproduction, and the cardinal symptom of bacterial wilt is：
The aerial partss of plant do not show any abnormalities phenomenon, daytime unexpected devitalization, whole overground part is all withered, cloudy day and sooner or later having
Recovered, such as healthy tree, however, in the near future just withering, in blue or green withered symptom, this process progress is very swift and violent.Due to turf
Bacterial wilt cannot be prevented, and bacterial wilt breaks with tremendous force, thus cultivated tomato is come using turf, once tomato seedling infection is blue or green withered
Disease, its survival rate is relatively low, more serious, tomato seedling even none survival.
Based on this, need the cultivation matrix for Fructus Lycopersici esculenti badly and improved.
Content of the invention
Present invention aims to deficiency of the prior art, and a kind of disease preventing type cultivation matrix and its preparation are provided
Methods and applications, the disease preventing type cultivation matrix being obtained using this preparation method, not only there is renewable, with low cost and environmental protection etc.
Advantage, and be applied in cultivated tomato, can preferably play the effect preventing and treating bacterial wilt, improve the survival of tomato seedling
Rate.
For achieving the above object, the technical scheme that the present invention takes is：A kind of disease preventing type cultivation matrix, by following weight ratio
Raw material make：
Compost 20-40%
Peat 50-70%
Perlite 5-15%
Described compost is made up of presoma and composite bacteria agent capable, and described composite bacteria agent capable accounts for the weight of presoma than for 0.1-
0.5%；
Described presoma is mainly made up of following raw material：Agriculture and forestry organic waste material, water, nitrogen source and phosphorus source, described water accounts for presoma
Weight than for 50-60%；
Described composite bacteria agent capable is mainly made by with hypothallus：Colloid bacillus cereus, bacillus subtilises, huge spore bar
Bacterium, Trichoderma viride, saccharomyces cerevisiae, candida mycoderma and proteolysis candida mycoderma.
Preferably, the weight proportion of the raw material of described disease preventing type cultivation matrix is：
Compost 30%
Peat 60%
Perlite 10%
Wherein, described composite bacteria agent capable accounts for the weight of presoma than for 0.3%.
Preferably, described agriculture and forestry organic waste material be sisal dregs, bagasse, manioc waste, straw, bean bar, Caulis et Folium Oryzae, rice husk, sawdust,
At least one in coconut husk.
Preferably, in every cubic metre of presoma, nitrogen source is 1-3kg.
It is furthermore preferred that nitrogen source is 2kg in every cubic metre of presoma.
Preferably, described nitrogen source is carbamide.
Preferably, in every cubic metre of presoma, phosphorus source is 3-7kg.
It is furthermore preferred that phosphorus source is 5kg in every cubic metre of presoma.
Preferably, phosphorus source is ground phosphate rock.
Preferably, described colloid bacillus cereus, bacillus subtilises, bacillus megaterium, Trichoderma viride, saccharomyces cerevisiae,
At least one of candida mycoderma and proteolysis candida mycoderma are single bacterium colony.
In addition, the present invention also provides the preparation method of above-mentioned disease preventing type cultivation matrix, comprise the following steps：
Forerunner's preparation step：Agriculture and forestry organic waste material, water, nitrogen source and phosphorus source are mixed and piles up, obtain final product described presoma；
Composite bacteria agent capable preparation process：Picking thalline adds culture medium and is fermented into bacterium solution, specifically：Picking colloid spore bar
Bacterium adds in silicate fluid medium, and picking bacillus subtilises and bacillus megaterium add the training of beef extract-peptone liquid
In foster base, picking Trichoderma viride, saccharomyces cerevisiae, candida mycoderma and proteolysis candida mycoderma add potato dextrose agar
In, allow each culture medium in the 20-35 DEG C of 24-96h that ferments, so that the thalline content in each culture medium is higher than 108cfu/mL
Stop fermentation, then in 120-180r min-1Under the conditions of centrifugation obtain each bacterium solution, then by each bacterium solution mix, be obtained described multiple
Close microbial inoculum；
Compost preparation process：After described presoma ferments 35-45 days, add institute by the 0.1-0.5% of forerunner's body weight
State composite bacteria agent capable, after the 50-55 days, obtain final product described compost；
Compounding step：Mix than by compost, peat and perlite by described weight, obtain final product described disease preventing type cultivation matrix.
Preferably, in described forerunner's preparation step：Anticipate agriculture and forestry organic waste material, so that its particle diameter is less than or equal to
3cm.
Preferably, in described forerunner's preparation step：Presoma is piled into cone shape presoma.
Preferably, in described composite bacteria agent capable preparation process：Each culture medium is fermented at 28 DEG C.
Preferably, in described composite bacteria agent capable preparation process：The time of each culture medium fermentation is 72h.
Preferably, in described composite bacteria agent capable preparation process：Each bacterium solution is pressed equal-volume ratio and is mixed.
Preferably, in described compost preparation process：Composite bacteria agent capable is uniformly sprayed onto on presoma, and turns over presoma and mix
Even.
Preferably, described compost preparation process is specially：After described presoma ferments 39 days, by forerunner's body weight
0.1-0.5% adds described composite bacteria agent capable, after the 53rd day, obtains final product described compost.
It is furthermore preferred that the 0.3% described composite bacteria agent capable of addition by forerunner's body weight.
Preferably, in described compost preparation process：Composite bacteria agent capable is uniformly sprayed onto on presoma, and turns over presoma and mix
Even.
Finally, the present invention also provides application on preparing anti-bacterial wilt pesticide for the above-mentioned disease preventing type cultivation matrix.
Preferably, described application is：Above-mentioned disease preventing type cultivation matrix answering on the pesticide of bacterial wilt preparing anti-vegetable
With.
Preferably, described application is：Above-mentioned disease preventing type cultivation matrix is used for the pesticide of the bacterial wilt of anti-Fructus Lycopersici esculenti in preparation
Application.
Beneficial effects of the present invention：
A kind of disease preventing type cultivation matrix of the present invention, on the one hand, due to being one of raw material using agriculture and forestry organic waste material, thus tool
There is renewable, with low cost and environmental protection, on the other hand, due to using described composite bacteria agent capable, and described raw material is pressed institute
State weight than preparation so that when described disease preventing type cultivation matrix is applied to cultivated tomato, preferably playing and prevent and treat bacterial wilt
Effect, improves the survival rate of tomato seedling.
The preparation method of a kind of disease preventing type cultivation matrix of the present invention, on the one hand, can prepare and there is diseases prevention as above
Type cultivation matrix, on the other hand, described preparation method can obtain intermediate product：Compost of the present invention, this compost has following
Advantage：(1) it is applied to cultivate seed, the germination index that can make seed is more than 100%；(2) compost of the present invention is inoculated with again
Close microbial inoculum, the content of humic acid of this compost is higher than the humic acid in the common compost do not inoculate in prior art described composite bacteria agent capable
Content；(3) compost of the present invention, compared with the common compost not inoculating described composite bacteria agent capable, accumulates the water solublity extracting 3 times
Phosphorus content increases by 25.65%；(4) compost of the present invention can improve group's species richness and microorganism overall activity in compost,
Specifically, microbiologic population's AWCD value of compost, Shannon inde compost and Mcintosh inde compost noticeably greater than do not connect
Plant the common compost of described composite bacteria agent capable, and Simpson inde compost no significant difference, show to add described composite bacteria agent capable pair
Common species impact in compost group is inconspicuous；(5) compost of the present invention can improve to four class utilization of carbon source abilities,
Biolog ECO plate has 31 kinds of single carbon sources, can be divided into four big class, respectively glucide and its ramification, aminoacid and its derivative
Thing, fatty acid and lipid and metabolic intermediate and secondary metabolitess, with the compost treatment phase not inoculating described composite bacteria agent capable
The compost of the present invention can significantly improve to four class utilization of carbon source abilities ratio.
A kind of application on preparation is for anti-bacterial wilt pesticide for the disease preventing type cultivation matrix of the present invention, due to adopting agricultural
Garbage and composite bacteria agent capable substitute turf of the prior art, not only achieve the treatment to bacterial wilt, and turn waste into wealth, will
Discarded agriculture and forestry organic waste material re-uses so that having cheap, environmental protection and can using this cultivation matrix of agriculture and forestry organic waste material
The advantages of regeneration.
Specific embodiment
Embodiment 1
The disease preventing type cultivation matrix of the embodiment of the present invention, is made up of the raw material of following weight ratio：
Compost 30%
Peat 60%
Perlite 10%
Described compost is made up of presoma and composite bacteria agent capable, and described composite bacteria agent capable accounts for the weight of presoma than for 0.3%；
Described presoma is mainly made up of following raw material：Straw, water, carbamide and ground phosphate rock, described water accounts for the weight of presoma
Amount ratio is 55%, nitrogen source content 2kg in every cubic metre of presoma, phosphorus source content 5kg in every cubic metre of presoma.Certainly, described
Presoma can also add potash fertilizer, compound fertilizer etc., and the present embodiment is not specifically limited.
Described composite bacteria agent capable is mainly made by with hypothallus：Colloid bacillus cereus, bacillus subtilises, huge spore bar
Bacterium, Trichoderma viride, saccharomyces cerevisiae, candida mycoderma and proteolysis candida mycoderma, all thalline are single bacterium colony.Certainly, except above-mentioned
Outside thalline, described composite bacteria agent capable can also add the thalline such as lactobacilluss, Bacillus licheniformis, and the present embodiment does not do concrete limit
Fixed.
The preparation method of the disease preventing type cultivation matrix of the embodiment of the present invention, comprises the following steps：
Forerunner's preparation step：Straw is pre-processed into the straw that particle diameter is less than or equal to 3cm, particle diameter is less than or waits
Mix and be piled into coniform in the straw of 3cm, water, carbamide and ground phosphate rock, obtain final product described presoma；
Composite bacteria agent capable preparation process：Picking thalline adds culture medium and is fermented into bacterium solution, specifically：Picking colloid spore bar
Bacterium adds in silicate fluid medium, and picking bacillus subtilises and bacillus megaterium add the training of beef extract-peptone liquid
In foster base, picking Trichoderma viride, saccharomyces cerevisiae, candida mycoderma and proteolysis candida mycoderma add potato dextrose agar
In, allow each culture medium in 25 DEG C of 72h that ferment, so that the thalline content in each culture medium is higher than 108cfu/mL stopping sending out
Ferment, then in 140r min-1Under the conditions of centrifugation obtain each bacterium solution, then by each bacterium solution mix, be obtained described composite bacteria agent capable；
Compost preparation process：After described presoma ferments 39 days, by forerunner's body weight 0.3% by described composite bacteria agent capable
Uniformly it is sprayed onto on presoma, and turns over presoma and mix, after the 53rd day, obtain final product described compost；
Compounding step：By weight, 30% compost, 60% peat and 10% perlite are mixed, obtain final product described disease preventing type
Cultivation matrix.
On the pesticide that the disease preventing type cultivation matrix of the embodiment of the present invention can apply to prepare the bacterial wilt of anti-Fructus Lycopersici esculenti.
Embodiment 2
The disease preventing type cultivation matrix of the embodiment of the present invention, is made up of the raw material of following weight ratio：
Compost 40%
Peat 55%
Perlite 15%
Described compost is made up of presoma and composite bacteria agent capable, and described composite bacteria agent capable accounts for the weight of presoma than for 0.5%；
Described presoma is mainly made up of following raw material：Caulis et Folium Oryzae, water, carbamide and ground phosphate rock, described water accounts for the weight of presoma
Amount ratio is 60%, nitrogen source content 3kg in every cubic metre of presoma, phosphorus source content 7kg in every cubic metre of presoma.Certainly, described
Presoma can also add potash fertilizer, compound fertilizer etc., and the present embodiment is not specifically limited.
Described composite bacteria agent capable is mainly made by with hypothallus：Colloid bacillus cereus, bacillus subtilises, huge spore bar
Bacterium, Trichoderma viride, saccharomyces cerevisiae, candida mycoderma and proteolysis candida mycoderma, all thalline are single bacterium colony.Certainly, except above-mentioned
Outside thalline, described composite bacteria agent capable can also add the thalline such as lactobacilluss, Bacillus licheniformis, and the present embodiment does not do concrete limit
Fixed.
The preparation method of the disease preventing type cultivation matrix of the embodiment of the present invention, comprises the following steps：
Forerunner's preparation step：Caulis et Folium Oryzae is pre-processed into the Caulis et Folium Oryzae that particle diameter is less than or equal to 3cm, particle diameter is less than or waits
Mix and be piled into coniform in the Caulis et Folium Oryzae of 3cm, water, carbamide and ground phosphate rock, obtain final product described presoma；
Composite bacteria agent capable preparation process：Picking thalline adds culture medium and is fermented into bacterium solution, specifically：Picking colloid spore bar
Bacterium adds in silicate fluid medium, and picking bacillus subtilises and bacillus megaterium add the training of beef extract-peptone liquid
In foster base, picking Trichoderma viride, saccharomyces cerevisiae, candida mycoderma and proteolysis candida mycoderma add potato dextrose agar
In, allow each culture medium in 28 DEG C of 86h that ferment, so that the thalline content in each culture medium stops higher than 108cfu/mL
Fermentation, then in 150r min-1Under the conditions of centrifugation obtain each bacterium solution, then by each bacterium solution mix, be obtained described composite bacteria agent capable；
Compost preparation process：After described presoma ferments 45 days, by forerunner's body weight 0.5% by described composite bacteria agent capable
Uniformly it is sprayed onto on presoma, and turns over presoma and mix, after the 55th day, obtain final product described compost；
Compounding step：By weight, 40% compost, 55% peat and 15% perlite are mixed, obtain final product described disease preventing type
Cultivation matrix.
Embodiment 3
The disease preventing type cultivation matrix of the embodiment of the present invention, is made up of the raw material of following weight ratio：
Compost 25%
Peat 70%
Perlite 5%
Described compost is made up of presoma and composite bacteria agent capable, and described composite bacteria agent capable accounts for the weight of presoma than for 0.1%；
Described presoma is mainly made up of following raw material：Sisal dregs, water, carbamide and ground phosphate rock, described water accounts for presoma
Weight than for 50%, nitrogen source content 1kg in every cubic metre of presoma, phosphorus source content 3kg in every cubic metre of presoma.Certainly, institute
State presoma and can also add potash fertilizer, compound fertilizer etc., the present embodiment is not specifically limited.
Described composite bacteria agent capable is mainly made by with hypothallus：Colloid bacillus cereus, bacillus subtilises, huge spore bar
Bacterium, Trichoderma viride, saccharomyces cerevisiae, candida mycoderma and proteolysis candida mycoderma, all thalline are single bacterium colony.Certainly, except above-mentioned
Outside thalline, described composite bacteria agent capable can also add the thalline such as lactobacilluss, Bacillus licheniformis, and the present embodiment does not do concrete limit
Fixed.
The preparation method of the disease preventing type cultivation matrix of the embodiment of the present invention, comprises the following steps：
Forerunner's preparation step：Sisal dregs are pre-processed into the sisal dregs that particle diameter is less than or equal to 3cm, particle diameter is less than
Or the sisal dregs equal to 3cm, water, carbamide and ground phosphate rock mix and is piled into coniform, obtain final product described presoma；
Composite bacteria agent capable preparation process：Picking thalline adds culture medium and is fermented into bacterium solution, specifically：Picking colloid spore bar
Bacterium adds in silicate fluid medium, and picking bacillus subtilises and bacillus megaterium add the training of beef extract-peptone liquid
In foster base, picking Trichoderma viride, saccharomyces cerevisiae, candida mycoderma and proteolysis candida mycoderma add potato dextrose agar
In, allow each culture medium in 24 DEG C of 24h that ferment, so that the thalline content in each culture medium stops higher than 108cfu/mL
Fermentation, then in 170r min-1Under the conditions of centrifugation obtain each bacterium solution, then by each bacterium solution mix, be obtained described composite bacteria agent capable；
Compost preparation process：After described presoma ferments 35 days, by forerunner's body weight 0.1% by described composite bacteria agent capable
Uniformly it is sprayed onto on presoma, and turns over presoma and mix, after the 50th day, obtain final product described compost；
Compounding step：By weight, 25% compost, 70% peat and 5% perlite are mixed, obtain final product described disease preventing type and plant
Training substrate.
The disease preventing type cultivation matrix of the embodiment of the present invention can apply to prepare the pesticide of the bacterial wilt for anti-Fructus Lycopersici esculenti.
Test example 1
Disease preventing type cultivation matrix in the embodiment of the present invention 1 and traditional matrices are done controlled trial, traditional matrices are not using
The compost of composite bacteria agent capable of the inoculation embodiment of the present invention, peat and perlite mix by weight 30%, 60% and 10% and prepare
One-tenth forms.Choose 20 potted flower basins, wherein 10 basins all fill 2kg disease preventing type cultivation matrix, in addition 10 basins equal 2kg dress traditional matrices, move
Plant 20 plants of Fructus Lycopersici esculenties, one plant of every basin, 7 days Fructus Lycopersici esculenti normal growths of transplanting seedlings.Result of the test is shown in Table 1- table 3.
Table 1 Fructus Lycopersici esculenti disease index
The 14th day substrate AWCD value of table 2 and microbial diversity (72h)
Note：In table, data is analyzed with SAS software, and multiple comparisons adopt Duncan method.Numerical value shown in table is 3 weights
Multiple meansigma methodss ± standard error, with the data in column data with same letter, there was no significant difference (p=0.05).
The 40th day substrate AWCD value of table 3 and microbial diversity (72h)
Note：In table, data is analyzed with SAS software, and multiple comparisons adopt Duncan method.Numerical value shown in table is 3 weights
Multiple meansigma methodss ± standard error, with the data in column data with same letter, there was no significant difference (p=0.05).
Table 4 Fructus Lycopersici esculenti overground part biomass (g)
Above-mentioned result of the test shows：
(1) the disease preventing type cultivation matrix in the embodiment of the present invention 1 can reduce the sickness rate of the bacterial wilt of Fructus Lycopersici esculenti.Specifically,
Table 1 shows, the Fructus Lycopersici esculenti of traditional matrices plantation fell ill at the 12nd day, and the Fructus Lycopersici esculenti of disease preventing type cultivation matrix plantation fell ill at the 16th day；
During to the 25th day, the Fructus Lycopersici esculenti of traditional substrate plantation all falls ill death, and the Fructus Lycopersici esculenti sickness rate of disease preventing type cultivation matrix plantation is
58.33%；During to the 40th day, the Fructus Lycopersici esculenti sickness rate of disease preventing type cultivation matrix plantation is 66.67%.
(2) in the disease preventing type cultivation matrix of the embodiment of the present invention 1, microorganism overall activity is strong, species richness and the uniformity
High.Specifically, it will be appreciated by the skilled addressee that AWCD directly reflects the overall activity of microorganism, Shannon inde heap
Fertile assessment group's species richness and the uniformity, the dominance that modal species in group assessed by Simpson inde compost refers to
Number, the tolerance of McIntosh inde compost Ze Shi group species homogeneity, 3 species diversity indexes reflect edaphon
The multifarious not ipsilateral of drop.Table 2 and table 3 show, the 14th day, the 40th day when the traditional AWCD value of substrate and
Shannoninde compost index is substantially less than disease preventing type cultivation matrix, shows microorganism overall activity in disease preventing type cultivation matrix
By force, species richness and the uniformity are high；And Simpson inde compost index and Mcintosh inde compost index are slightly less than and prevent
Sick type cultivation matrix, shows that interpolation composite bacteria agent capable affects on common species in substrate group inconspicuous.
(3) adopt the tomato plant Biomass of the disease preventing type cultivation matrix plantation of the embodiment of the present invention 1 high.Specifically, such as
Shown in table 4, tomato planting, after 40 days, harvests overground part measurement biomass, compared with using traditional matrices plantation, uses diseases prevention
The Fructus Lycopersici esculenti overground part biomass of type cultivation matrix plantation is high.
Test example 2
By the compost (i.e. inoculating compound bacterium agent) in the embodiment of the present invention 1 with do not inoculate compound bacteria in the embodiment of the present invention 1
The common compost (i.e. not inoculating compound bacterium agent) of agent is contrasted, and tests its index of correlation, as shown in table 5：
The index of correlation of the 53rd day each composting production of table 5 measures
Above-mentioned result of the test (table 5) shows：
The inoculating compound bacterium agent (i.e. compost) of the embodiment of the present invention 1 can improve the Utilization ability to four class carbon sources.
Biolog ECO plate has 31 kinds of single carbon sources, can be divided into four big class, respectively glucide and its ramification, aminoacid and its derivative
Thing, fatty acid and lipid and metabolic intermediate and secondary metabolitess.Result of the test shows：Heap with not inoculating compound bacterium agent
Fertile process is compared, and the compost (i.e. compost) of inoculating compound bacterium agent can significantly improve the Utilization ability to four class carbon sources.
Finally it should be noted that above example is only in order to illustrating technical scheme, rather than the present invention is protected
The restriction of shield scope, although having made to explain to the present invention with reference to preferred embodiment, those of ordinary skill in the art should
Work as understanding, technical scheme can be modified or equivalent, without deviating from the reality of technical solution of the present invention
Matter and scope.
Claims (10)
1. a kind of disease preventing type cultivation matrix is it is characterised in that it is made up of the raw material of following weight ratio：
Compost 20-40%
Peat 50-70%
Perlite 5-15%
Described compost is made up of presoma and composite bacteria agent capable, and described composite bacteria agent capable accounts for the weight of presoma than for 0.1-0.5%；
Described presoma is mainly made up of following raw material：Agriculture and forestry organic waste material, water, nitrogen source and phosphorus source, described water accounts for the weight of presoma
Amount ratio is 50-60%；
Described composite bacteria agent capable is mainly made by with hypothallus：Colloid bacillus cereus, bacillus subtilises, bacillus megaterium, green
Color Trichoderma spp., saccharomyces cerevisiae, candida mycoderma and proteolysis candida mycoderma.
2. disease preventing type cultivation matrix as claimed in claim 1 is it is characterised in that nitrogen source content 1-3kg in every cubic metre of presoma.
3. disease preventing type cultivation matrix as claimed in claim 1 is it is characterised in that phosphorus source content 3-7kg in every cubic metre of presoma.
4. disease preventing type cultivation matrix as claimed in claim 1 it is characterised in that described colloid bacillus cereus, bacillus subtilises,
At least one of bacillus megaterium, Trichoderma viride, saccharomyces cerevisiae, candida mycoderma and proteolysis candida mycoderma are single bacterium colony.
5. a kind of preparation method of disease preventing type cultivation matrix as described in any one as claim 1-4 is it is characterised in that include following
Step：
Forerunner's preparation step：Agriculture and forestry organic waste material, water, nitrogen source and phosphorus source are mixed and piles up, obtain final product described presoma；
Composite bacteria agent capable preparation process：Picking thalline adds culture medium and is fermented into bacterium solution, specifically：Picking colloid bacillus cereus add
Enter in silicate fluid medium, picking bacillus subtilises and bacillus megaterium add beef extract-peptone fluid medium
In, picking Trichoderma viride, saccharomyces cerevisiae, candida mycoderma and proteolysis candida mycoderma add in potato dextrose agar,
Allow each culture medium in the 20-35 DEG C of 24-96h that ferments, so that the thalline content in each culture medium stops higher than 108cfu/mL
Fermentation, then in 120-180r min-1Under the conditions of centrifugation obtain each bacterium solution, then by each bacterium solution mix, be obtained described compound bacteria
Agent；
Compost preparation process：After described presoma ferments 35-45 days, add described compound by the 0.1-0.5% of forerunner's body weight
Microbial inoculum, after the 50-55 days, obtains final product described compost；
Compounding step：Mix than by compost, peat and perlite by described weight, obtain final product described disease preventing type cultivation matrix.
6. the preparation method of disease preventing type cultivation matrix as claimed in claim 5 is it is characterised in that described composite bacteria agent capable preparation process
In：Each culture medium is all fermented at 28 DEG C.
7. the preparation method of disease preventing type cultivation matrix as claimed in claim 5 is it is characterised in that described composite bacteria agent capable preparation process
In：Each culture medium is all fermented 72h.
8. the preparation method of disease preventing type cultivation matrix as claimed in claim 5 is it is characterised in that described composite bacteria agent capable preparation process
In：Each bacterium solution is pressed equal-volume ratio and is mixed.
9. the preparation method of disease preventing type cultivation matrix as claimed in claim 5 is it is characterised in that in described compost preparation process：
Composite bacteria agent capable is uniformly sprayed onto on presoma, and stirs presoma and mix.
10. application on preparation is for anti-bacterial wilt pesticide for the disease preventing type cultivation matrix as described in any one of claim 1-5.
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