CN105948831A - Method for producing biocontrol fertilizer by means of brewer's grain - Google Patents
Method for producing biocontrol fertilizer by means of brewer's grain Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/006—Waste from chemical processing of material, e.g. diestillation, roasting, cooking
- C05F5/008—Waste from biochemical processing of material, e.g. fermentation, breweries
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention relates to a method for producing a fungus biocontrol fertilizer by means of brewer's grain and belongs to the technical fields of microbial pesticides and agricultural fertilizers. The biocontrol fungus strain is Beauveria bassiana. The method for producing the fungus biocontrol fertilizer mainly comprises the steps of seed preparation, semi-solid fermentation, solid fermentation and aftertreatment, wherein brewer's grain serves as the main carbon and nitrogen source and carrier during semi-solid fermentation and solid fermentation, so that the solid biocontrol fertilizer is prepared. A new approach is provided for recycling brewer's grain waste, and the produced biocontrol fertilizer can promote growth of plants and has a good effect of controlling various plant pests.
Description
Technical field
The invention belongs to agricultural fertilizer technical field, be specifically related to a kind of microorganism biological and ecological methods to prevent plant disease, pests, and erosion fertilizer and
Its preparation method, the high-effective microorganism biological and ecological methods to prevent plant disease, pests, and erosion fertilizer of a kind of pest-resistant growth-promoting.
Background technology
Along with the development of China's economic, as the in the world first big alcoholic beverage, the medicated beer of China
Consumption figure the most progressively strengthens.According to statistics, within 2014, Chinese beer yield reaches 4921.85 ten thousand kilolitres, is
Beer Production state maximum in the world.According to statistics, often produce 1 ton of medicated beer, need to produce distiller's grains of beer about
0.25 ton.Distiller's grains of beer are mainly beer raw material Fructus Hordei Germinatus, rice, and Flos lupuli (Flos Humuli Lupuli) through saccharifying gelatinization process
After, carry out filtering the waste material that squeezing obtains.Moisture in distiller's grains of beer is about 50%～85%, contains simultaneously
Having abundant nutritional labeling, wherein, crude protein accounts for the 25% of dry weight, crude fat about 10%, crude fibre
About 8%, nitrogen-free extract about 55%, and the ash of 2%.But owing to wherein moisture is higher,
Nutritious, there is corruption so as easy as rolling off a log.At present, distiller's grains of beer many by direct sale at low prices to surrounding
Peasant, as the feedstuff of stock raising；It is directly dried process by another part, then after carrying out
Continuous feed manufacturing processes, and energy resource consumption is excessive；What is more directly discharges it, serious environment pollution.
It would therefore be highly desirable to develop and utilize for distiller's grains of beer, further improve the added value of distiller's grains of beer.
Biocontrol fungi is a kind of to invade insect bodies endoparasitism, make the insecticide lethal fungus of morbidity,
It is a very important environmental microorganism of class, in preventing and treating harmful organism, there is wide development potentiality.
Beauveria bassiana (Beauveria bassiana) is the biocontrol fungi of a kind of broad spectrum activity, its active ingredient
Be include spore or mycelia infect body, typically invade haemocoele from insect body wall, in utilizing host's haemocoele
Nutrition and breed or excrete poison, insect is dead because of nutrition exhaustion or toxemia.Study people both at home and abroad
Member utilizes beauveria bassiana to prevent and treat Pyrausta nubilalis (Hubern)., pine moth, little sugarcane borer, blind Chinese toon, grain weevil, the red spider of mandarin orange
The agriculture and forestry injurious insect such as spider and aphid.Particularly to Pyrausta nubilalis (Hubern). and the Biological control of pine moth, the most make
Use for conventional means year after year.Owing to beauveria bassiana can efficiently control population number, do not hinder simultaneously
Evil other natural enemy insects and beneficial organism, comply fully with the objective of integrated pest management, simultaneously by
In its most a large amount of productions, cost accounting is more competitive, thus it is with a wide range of applications.
Additionally, it has been investigated that, beauveria bassiana is to sclerotinia rot of colza pathogenic bacteria, cotton wilt pathogenic bacteria and little
The pathogen such as wheat head blight there is certain antagonism.
Crossing patent disclosed in prior art is " solid medium of a kind of muscardine and muscardine
Cultural method ", Publication No. 103881930A.Wherein, the solid medium of this invention includes by matter
Amount than 1:(1～1.2): Vermiculitum, Testa Tritici and the Semen Maydis powder of (1～1.2), by Vermiculitum, Testa Tritici and
Semen Maydis powder mixed vapour sterilizing 40min obtains；On this basis, muscardine inoculation liquid is existed by this invention
After fluid enlargement culture base cultivation added with gentamycin sulfate, it is inoculated in solid medium,
Gather in the crops after 25～27 DEG C of cultivations 7.5～8d；The most also thank tail feather, Chen Hongmei etc., " utilize rice solid
The process optimization of state fermenting and producing beauveria bassiana " food and fermentation industries, 2007,33 (10).Its
In, this paper is using rice as solid-state fermentation culture medium, and inoculum concentration is 15% (v/w), cultivation temperature 25 DEG C,
Before ambient humidity, 4d is 100%, and middle 3d is 80%, and rear 3d is 60%, cultivates 10d.
In above-mentioned patent and paper, employ the people and animals such as rice, Testa Tritici, Semen Maydis powder in a large number edible
Agricultural product, add the consumption of grain resource；Solid culture based component therein is numerous and diverse, cost
Higher；All using beauveria bassiana spore powder as end product, numerous and diverse operating procedure need to be carried out to spore powder
Individually separate and recover, and original solid culture Quito is discarded again；And be all not directed to plant
The fertilizer field of thing growth-promoting.
Therefore, substantial amounts of distiller's grains of beer how are utilized to become urgently to be resolved hurrily asking to cultivate biocontrol fungi
Topic.
Summary of the invention
The technical problem to be solved is to overcome the deficiencies in the prior art, it is provided that a kind of
Utilize the method that the useless grain of medicated beer produces biological and ecological methods to prevent plant disease, pests, and erosion fertilizer.
The distiller's grains of beer waste material that the present invention produces with beer fermentation, as culture medium, is cultivated insecticidal power and is shown
Fertilizer after the beauveria bassiana write, and fermentation has more rich nutrient substance, it is possible to effectively promote
Enter the growth of plant, realize the preventing and treating to insect pest of the plant simultaneously, decrease chemical pesticide at plant growing
During the food-safety problem that causes.
The concrete technical scheme of the present invention is as follows:
A kind of method utilizing distiller's grains of beer to produce biological and ecological methods to prevent plant disease, pests, and erosion fertilizer, it is characterised in that: concrete steps are such as
Under:
(1) prepared by seed: accessed by beauveria bassiana in SADY fluid medium, and inoculum concentration is
1～3%, at 25～28 DEG C, the shaking table of 150～180r/min is cultivated 3～7d, it is thus achieved that seed liquor；
(2) semi-solid ferment: semisolid culture medium will be added in fermentation tank, consisting of:
The young beer grain of 50～150g, 5～40g glucoses, it is settled to 1L with water.Heated and boiled 15～30min,
To eliminate miscellaneous bacteria in culture medium.After being cooled to room temperature, inoculation 0.5～5% seed liquor, in 25～28 DEG C
Ventilate aerobic cultivation 24～72h, it is thus achieved that semi-solid ferment culture fluid；
(3) solid fermentation: take young beer grain, add the original sugarcane accounting for distiller's grains of beer 0.5～2wt%
Icing Sugar fully mixes, and toasts 30～45min in 80 DEG C, and reduction distiller's grains of beer humidity, to 50～65%, cools down standby
With；
The biocontrol fungi semisolid culture fluid obtained in step (2) is added according to the ratio of 10～20%
Enter in the solid medium obtained in step (3), mix homogeneously；By postvaccinal solid medium,
It is put in 25～28 DEG C of isoperibols sealing and cultivates 8～15d, be then aerated desiccation culture 3～5d, treat
Moisture content in medium is less than 15%, and when there is a large amount of beauveria bassiana spore powder, can obtain purpose biological and ecological methods to prevent plant disease, pests, and erosion fertile
Material.
In this step, distiller's grains of beer toast 30～45min at 80 DEG C, can realize disinfecting action；
The strain of beauveria bassiana (Beauveria bassiana) described in step (1) is commercially available ball
Beauveria bassiana microbial inoculum, as Jiangxi Tianren Ecology Co., Ltd. " general " beauveria bassiana,
" the more biological " beauveria bassiana etc. of the more bio tech ltd, Xiamen.
SADY fluid medium preparation method described in step (1) is as follows: glucose 4g,
Peptone 1g, yeast extract powder 1g, be settled to 100mL with water, after high temperature sterilize, is cooled to room temperature
Standby.
Biological and ecological methods to prevent plant disease, pests, and erosion fertilizer owing to obtaining in step (3) is block, and using for convenience can be by bulk
Biological and ecological methods to prevent plant disease, pests, and erosion fertilizer carry out break process, biological and ecological methods to prevent plant disease, pests, and erosion fertilizer finished product can be obtained.
The invention have the benefit that
1. the biological and ecological methods to prevent plant disease, pests, and erosion fertilizer of the present invention is to be formed through fermentation of white muscardine fungi by distiller's grains of beer, it is possible to effective
Improve soil microenvironment, killing off plant insect pest, it is provided that the nutrient substance needed for plant growing, for
Crop growth provides beneficial conditions.
2. use distiller's grains of beer as the culture medium of biocontrol fungi, main reason is that it is as micro-life
Thing culture medium, had both contained the residual sugar carbon source as growth of microorganism, had contained again abundant crude protein conduct
Nitrogen source needed for growth of microorganism, also contain simultaneously the calcium needed for growth of microorganism, phosphorus, manganese, ferrum,
The trace element such as copper, are a kind of without additionally adding or only need to add the outstanding of other chemical agents on a small quantity
Microbiological culture media.Additionally, after fermentation of white muscardine fungi embraced by ball, the crude protein contained in distiller's grains of beer
Progressively being decomposed into little molecule inorganic nitrogen Deng material, beneficially plant directly absorbs.Meanwhile, distiller's grains of beer
In the calcium that contains, phosphorus, manganese, ferrum, the trace element such as copper be also nutrient substance necessary to plant growing.
3. experiment shows, uses the biological and ecological methods to prevent plant disease, pests, and erosion fertilizer of the present invention can promote plant seed germination and life
Long, grain yield 10～35% can be improved through field experiment, improve yield of vegetables 15～35%, it is also possible to have
The quality improving grain vegetables of effect and local flavor.The fertilizer of every mu of land use present invention about 200～300g,
It is current amount of application less microorganism biological and ecological methods to prevent plant disease, pests, and erosion fertilizer.Time specifically used, biological and ecological methods to prevent plant disease, pests, and erosion fertilizer of the present invention adds
100～200 times of water, activate 12h in 22～28 DEG C, then use chemical fertilizer (normal usage amount together with former
50%) be sprinkled in soil.
In sum, the present invention is effectively cheap by produce in a large number in medicated beer actual production
Waste material distiller's grains of beer carry out development of resources recycling, effectively avoid because distiller's grains of beer are dealt with improperly
The environmental health problem caused, meanwhile, further improves the added value of distiller's grains of beer, for distiller's grains of beer
High-value-use provide new approaches；Using distiller's grains of beer as biocontrol fungi culture medium, and non-usage
The edible agricultural product of people and animals such as rice, Testa Tritici, Semen Maydis powder, decrease the consumption of grain resource；Logical
Cross that distiller's grains of beer fermenting and producing parasite killing strength, endurance is strong, environmental friendliness, the ball spore Bombyx Batryticatus of person poultry harmless
Bacterium, it is possible to as the substitute of the chemical pesticide the most excessively used, effective its usage amount of minimizing,
Thus avoid due to chemical pesticide use excessively cause soil, water source, the environmental pollution such as air, with
And the food-safety problem such as pesticide residues；The distiller's grains of beer beauveria bassiana mixture obtained after fermentation is entered
The simple processing of row, eliminates spore powder recycling step, and it had both had the abundant nutrition thing from distiller's grains of beer
Matter, possesses again the insecticidal effect of biocontrol fungi, is a kind of excellent bio-feritlizer.
Detailed description of the invention
The detailed description of the invention of form by the following examples, the foregoing of the present invention is done into
The detailed description of one step, but this should not being interpreted as, the scope of the above-mentioned theme of the present invention is only limitted to following
Example.All technology realized based on foregoing of the present invention belong to the scope of the present invention.
Embodiment 1
1. prepared by seed: select beauveria bassiana (Beauveria bassiana) as biological and ecological methods to prevent plant disease, pests, and erosion
Fungus seed bacterial strain, can be selected for " general " ball of commercially available Jiangxi Tianren Ecology Co., Ltd.
The microbial inoculums such as " the more biological " beauveria bassiana of beauveria bassiana, Xiamen the more bio tech ltd.
Beauveria bassiana is accessed SADY fluid medium (glucose 4g, peptone 1g, yeast extract powder
1g, is settled to 100mL with water, after high temperature sterilize, is cooled to room temperature, standby) in, inoculum concentration is
3%；At 26 DEG C, the shaking table of 150r/min is cultivated 4d, it is thus achieved that seed liquor, standby.
2. semi-solid ferment: will add semisolid culture medium in fermentation tank, its proportion of composing is:
The young beer grain of 100g, 20g glucose, it is settled to 1L with water, heated and boiled 30min, half-and-half
Solid medium carries out sterilizing, after being cooled to room temperature, inoculates semisolid culture medium 3% seed liquor, in
27 DEG C of aerobic cultivation 24h that ventilate, it is thus achieved that semi-solid ferment culture fluid；
3. solid fermentation: take young beer grain, adds 1wt% original sugarcane Icing Sugar and fully mixes, in
Drying 45min for 80 DEG C, reduction distiller's grains of beer humidity, to 50%, cools down standby；
The biocontrol fungi semisolid culture fluid obtained adds in solid medium according to the ratio of 20%,
Mix homogeneously；By postvaccinal solid medium, it is put in 27 DEG C of isoperibols sealing and cultivates 10d,
Then it is aerated cultivating 5d, treats that moisture content in medium is down to less than 15%, and there is a large amount of muscardine
During spore powder, purpose biological and ecological methods to prevent plant disease, pests, and erosion fertilizer can be obtained.
4. subsequent treatment: the biological and ecological methods to prevent plant disease, pests, and erosion fertilizer of in bulk is carried out break process, can obtain biological and ecological methods to prevent plant disease, pests, and erosion fertile
Material finished product.
Embodiment 2
1. prepared by seed: select beauveria bassiana (Beauveria bassiana) as biological and ecological methods to prevent plant disease, pests, and erosion
Fungus seed bacterial strain, can be selected for " general " ball of commercially available Jiangxi Tianren Ecology Co., Ltd.
The microbial inoculums such as " the more biological " beauveria bassiana of beauveria bassiana, Xiamen the more bio tech ltd.
Beauveria bassiana is accessed SADY fluid medium (glucose 4g, peptone 1g, yeast extract powder
1g, is settled to 100mL with water, after high temperature sterilize, is cooled to room temperature, standby) in, inoculum concentration is
2%, at 25 DEG C, the shaking table of 160r/min is cultivated 5d, it is thus achieved that seed liquor, standby.
2. semi-solid ferment: will add semisolid culture medium in fermentation tank, its proportion of composing is:
The young beer grain of 150g, 10g glucose, it is settled to 1L with water, heated and boiled 20min, half-and-half
Solid medium carries out sterilizing, after being cooled to room temperature, inoculates semisolid culture medium 5% seed liquor, in
26 DEG C of aerobic cultivation 24h that ventilate, it is thus achieved that semi-solid ferment culture fluid；
3. solid fermentation: take young beer grain, adds 1.5wt% original sugarcane Icing Sugar and fully mixes,
Drying 35min in 80 DEG C, reduction distiller's grains of beer humidity, to 60%, cools down standby；The biocontrol fungi that will obtain
Semisolid culture fluid adds in solid medium according to the ratio of 15%, mix homogeneously；By postvaccinal solid
Body culture medium, is put in 26 DEG C of isoperibols sealing and cultivates 12d, be then aerated cultivating 3d, treat
Moisture content in medium is down to less than 15%, and when there is a large amount of beauveria bassiana spore powder, can obtain purpose raw
Anti-fertilizer.
4. subsequent treatment: the biological and ecological methods to prevent plant disease, pests, and erosion fertilizer of in bulk is carried out break process, can obtain biological and ecological methods to prevent plant disease, pests, and erosion fertile
Material finished product.
Embodiment 3
1. prepared by seed: select beauveria bassiana (Beauveria bassiana) as biological and ecological methods to prevent plant disease, pests, and erosion
Fungus seed bacterial strain, can be selected for " general " ball of commercially available Jiangxi Tianren Ecology Co., Ltd.
The microbial inoculums such as " the more biological " beauveria bassiana of beauveria bassiana, Xiamen the more bio tech ltd.
Beauveria bassiana is accessed SADY fluid medium (glucose 4g, peptone 1g, yeast extract powder
1g, is settled to 100mL with water, after high temperature sterilize, is cooled to room temperature, standby) in, inoculum concentration is
2%, at 25 DEG C, the shaking table of 160r/min is cultivated 5d, it is thus achieved that seed liquor, standby.
2. semisolid state fermentation: will add semisolid culture medium in fermentation tank, its proportion of composing is:
The young beer grain of 150g, 10g glucose, it is settled to 1L with water, heated and boiled 20min, half-and-half
Solid medium carries out sterilizing, after being cooled to room temperature, inoculates semisolid culture medium 5% seed liquor, in
26 DEG C of aerobic cultivation 24h that ventilate, it is thus achieved that semi-solid ferment culture fluid；
3. solid fermentation: take young beer grain, adds 1.5wt% original sugarcane Icing Sugar and fully mixes,
Drying 35min in 80 DEG C, reduction distiller's grains of beer humidity, to 60%, cools down standby；The biocontrol fungi that will obtain
Semisolid culture fluid adds in solid medium according to the ratio of 10%, mix homogeneously；By postvaccinal solid
Body culture medium, is put in 26 DEG C of isoperibols sealing and cultivates 14d, be then aerated cultivating 3d, treat
Moisture content in medium is down to less than 15%, and when there is a large amount of beauveria bassiana spore powder, can obtain purpose raw
Anti-fertilizer.
4. subsequent treatment: the biological and ecological methods to prevent plant disease, pests, and erosion fertilizer of in bulk is carried out break process, can obtain biological and ecological methods to prevent plant disease, pests, and erosion fertile
Material finished product.
Embodiment 4 biocontrol fungi sporulation quantity detects
The biological and ecological methods to prevent plant disease, pests, and erosion fertilizer 5g submergence obtained in Example 1, embodiment 2, embodiment 3 respectively
In the 500ml sterilized water containing 2wt% tween 80, it is thoroughly mixed, after 30min, enters
Use blood counting chamber to count under the microscope after a certain proportion of dilution of row, calculate its sporulation quantity.By
Table 1 can obtain, and in this biological and ecological methods to prevent plant disease, pests, and erosion fertilizer, biocontrol fungi sporulation quantity is more, and up to 8.9 × 1011Individual/g, explanation
The present invention use distiller's grains of beer be very suitable for biocontrol fungi growth as solid medium, and relatively
High biocontrol fungi sporulation quantity is the basic guarantee of insecticidal effect.
Table 1 biological and ecological methods to prevent plant disease, pests, and erosion fertilizer sporulation quantity detection table
Embodiment 5 biocontrol fungi germination rate detects
Table 2 biological and ecological methods to prevent plant disease, pests, and erosion fertilizer germination rate detection table
The biological and ecological methods to prevent plant disease, pests, and erosion fertilizer 1g obtained in Example 1, embodiment 2, embodiment 3 respectively is immersed in 100ml
In sterilized water, it is sufficiently stirred for, counted under microscope, with sterilized water by its strain concentration dilution to 107
Individual/mL.Often group biological and ecological methods to prevent plant disease, pests, and erosion fertilizer sample samples 5, is respectively placed in 26 DEG C of constant temperature culture 24h, Yu Xian
Its germination rate of micro-Microscopic observation.Experiment in triplicate, is averaged.Can be obtained by table 2, this biological and ecological methods to prevent plant disease, pests, and erosion is fertile
Material produces spore germination rate and reaches more than 98%, it is possible to effective germination and growth, and this solid medium is described
The spore germination of generation can't be affected, and higher germination rate can effectively guarantee to infect the spore of insect
Son can play the effect of parasite killing.
Embodiment 6 biocontrol fungi virulence determination
The 1g biological and ecological methods to prevent plant disease, pests, and erosion fertilizer obtained in Example 1, embodiment 2, embodiment 3 is immersed in 100ml
In sterilized water, it is sufficiently stirred for, counted under microscope, with sterilized water by its strain concentration dilution to 107
Individual/mL.Using greater wax moth as virulence determination experimental subject, take greater wax moth larva 200,50 one group,
Carry out packet to observe.All it is soaked in 10s in biological and ecological methods to prevent plant disease, pests, and erosion fertilizer diluent by often organizing greater wax moth, takes out and be put in room
Temperature, the survival number of observed and recorded its every day, calculate average viability every day.Experimental result can obtain, this
Biological and ecological methods to prevent plant disease, pests, and erosion fertilizer median lethal time LT50For 3.8d, there is stronger insecticidal toxicity, it is possible to effectively preventing
Plant insect.
Embodiment 7 plant growth-promoting ability detects
The biological and ecological methods to prevent plant disease, pests, and erosion fertilizer promotion growth to plant is detected with soybean germination.Take 600 greatly
Bean, every 100 as one group, observe.Soybean seed is soaked in 10min in 60 ° of ethanol,
Taking-up washes down.Respectively by embodiment 1, embodiment 2, embodiment 3 obtain 1g biological and ecological methods to prevent plant disease, pests, and erosion fertilizer and
Dry beer vinasse is immersed in 20ml sterilized water, is thoroughly mixed, standby, the biological and ecological methods to prevent plant disease, pests, and erosion of each embodiment
Fertilizer all does 3 groups of parallel tests.Then by Semen sojae atricolor according to the ratio of 1: 3mL be immersed in respectively water,
8h in distiller's grains of beer, fertilizer leachate, is positioned in 25～26 DEG C of constant incubators after taking out subsequently, keeps away
Light is cultivated.Observed and recorded often organizes Semen sojae atricolor plumule shaft length average every day.Experimental result shows, this biological and ecological methods to prevent plant disease, pests, and erosion
Fertilizer has certain facilitation to Semen sojae atricolor growth, it is possible to play the effect of fertilizer promoting plant growing,
And effect is obvious.
Claims (1)
1. one kind utilizes the method that distiller's grains of beer produce biological and ecological methods to prevent plant disease, pests, and erosion fertilizer, it is characterised in that: specifically comprise the following steps that
(1) prepared by seed: accessed by beauveria bassiana in SADY fluid medium, and inoculum concentration is 1～3%,
At 25～28 DEG C, the shaking table of 150～180r/min is cultivated 3～7d, it is thus achieved that seed liquor；
(2) semi-solid ferment: semisolid culture medium will be added in fermentation tank, consisting of: 50～150g
Young beer grain, 5～40g glucoses, be settled to 1L with water, heated and boiled 15～30min, to disappear
Miscellaneous bacteria in culture medium of going out, after being cooled to room temperature, inoculation 0.5～5% seed liquor, in 25～28 DEG C of ventilations
Aerobic cultivation 24～72h, it is thus achieved that semi-solid ferment culture fluid；
(3) solid fermentation: take young beer grain, add the original sugarcane Icing Sugar accounting for distiller's grains of beer 0.5～2wt%
Fully mixing, toasts 30～45min in 80 DEG C, and reduction distiller's grains of beer humidity, to 50～65%, cools down standby；
By the biocontrol fungi semisolid culture fluid obtained in step (2) according to the inoculum concentration of 10～20% enter to
In the solid medium obtained in step (3), mix homogeneously；By postvaccinal solid medium, put
Seal in 25～28 DEG C of isoperibols and cultivate 8～15d, be then aerated desiccation culture 3～5d, wait to train
Support base water content and be less than 15%, and when there is a large amount of beauveria bassiana spore powder, purpose biological and ecological methods to prevent plant disease, pests, and erosion fertilizer can be obtained.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610407632.9A CN105948831B (en) | 2016-06-07 | 2016-06-07 | A method of biological and ecological methods to prevent plant disease, pests, and erosion fertilizer is produced using brewex's grains |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610407632.9A CN105948831B (en) | 2016-06-07 | 2016-06-07 | A method of biological and ecological methods to prevent plant disease, pests, and erosion fertilizer is produced using brewex's grains |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108624512A (en) * | 2018-05-30 | 2018-10-09 | 内蒙古和盛生态科技研究院有限公司 | Solid fermentation matrix, preparation method and the method for cultivating mycorhiza biological agent |
| CN113373067A (en) * | 2021-07-28 | 2021-09-10 | 贵州省烟草公司遵义市公司 | Compound microbial agent capable of promoting plant growth and application thereof |
| CN113698254A (en) * | 2020-05-07 | 2021-11-26 | 交城县田丰肥业有限公司 | Multi-organic-matter polymerized fertilizer and preparation process thereof |
| CN115053916A (en) * | 2022-06-29 | 2022-09-16 | 济南大学 | Microbial agent capable of improving stability of beauveria bassiana and preparation method thereof |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024003323A1 (en) * | 2022-06-29 | 2024-01-04 | Mushlabs Gmbh | Production of a fungal fermentation medium from brewer's spent grain |
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| CN103086760A (en) * | 2013-01-11 | 2013-05-08 | 李继生 | Preparation method of ecological fertilizer |
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| CN103086760A (en) * | 2013-01-11 | 2013-05-08 | 李继生 | Preparation method of ecological fertilizer |
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| 王海燕等: ""酒糟废渣发酵生产有机肥的研究"", 《酿酒科技》 * |
| 谢翎 等: ""利用大米固态发酵生产球球孢白僵菌的工艺优化"", 《食品与发酵工业》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108624512A (en) * | 2018-05-30 | 2018-10-09 | 内蒙古和盛生态科技研究院有限公司 | Solid fermentation matrix, preparation method and the method for cultivating mycorhiza biological agent |
| CN108624512B (en) * | 2018-05-30 | 2021-03-30 | 内蒙古和盛生态科技研究院有限公司 | Solid fermentation substrate, preparation method and method for culturing mycorrhiza biological preparation |
| CN113698254A (en) * | 2020-05-07 | 2021-11-26 | 交城县田丰肥业有限公司 | Multi-organic-matter polymerized fertilizer and preparation process thereof |
| CN113373067A (en) * | 2021-07-28 | 2021-09-10 | 贵州省烟草公司遵义市公司 | Compound microbial agent capable of promoting plant growth and application thereof |
| CN115053916A (en) * | 2022-06-29 | 2022-09-16 | 济南大学 | Microbial agent capable of improving stability of beauveria bassiana and preparation method thereof |
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| CN105948831B (en) | 2019-08-27 |
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