CN104609924A - Preparation method and application of special nutrient solution for blueberry rhizospheric microorganism activation - Google Patents
Preparation method and application of special nutrient solution for blueberry rhizospheric microorganism activation Download PDFInfo
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- CN104609924A CN104609924A CN201510025598.4A CN201510025598A CN104609924A CN 104609924 A CN104609924 A CN 104609924A CN 201510025598 A CN201510025598 A CN 201510025598A CN 104609924 A CN104609924 A CN 104609924A
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention discloses a preparation method and an application of a special nutrient solution for blueberry rhizospheric microorganism activation. Organic-selenium-enriched germ powder bred from selenium-enriched astragalus sinicus seeds, okra and corn is added to a fermentation medium with a microbial technology, and then various probiotics such as methane bacteria, mixed yeast, lactobacillus, bacillus subtilis and the like are centralized in the medium. An active bacterium agent with the PH ranging from 4.0-4.5 and containing selenium-enriched methane bacteria, the mixed yeast and the bacillus subtilis is produced through simulation of soil variable-temperature anaerobic and aerobic fermentation, at least 5000,000-8000,000 strains exist per milliliter, and the bacterium agent is dropped in the blueberry rhizospheric range in the amount of 45 L/ha.
Description
Technical field
The invention belongs to the research of " blueberry green organic manure " new technology, be specifically related to a kind of activation blueberry rhizospheric microorganism special nutritional liquid and preparation method thereof and application.
Background technology
Blueberry is that a kind of economic worth is high, the unique health-care effect of tool, and is suitable for the emerging small berries of China's red-yellow soil regional development, more and more in widespread attention in world's fruit at present.Blueberry majority is shrub, and rare is dungarunga.Because kind is different, the height of blueberry plant often has larger difference, and therefore cultivation technique also has very big-difference.The high clump blueberry height of tree, generally at about 2m, reaches as high as 7 ~ 8m, and the short clump blueberry height of tree is mostly at below 1m, and the eugonic Vaccinium ashei height of tree can be greater than high clump blueberry.High clump blueberry and Vaccinium ashei are mainly artificial culture, and short clump blueberry is essentially wild.According to the ecological suitability of blueberry, the cultivation of blueberry first should determine suitable cultivation region, i.e. weather condition Suitable Area, then carries out the selection of planting plot.When selecting plantation plot first, understand or measure soil pH value; Secondly, loosing soil to be selected as far as possible, be rich in organic matter and the good place of irrigation condition.
Plant and rhizospheric microorganism, as an ecosystem mutual reciprocity and mutual benefit, interact in the process of common evolutionary, and this is a long-term process selected mutually.It can promote host plant to absorb nutritive ingredient, helps host plant to expand nutritive ingredient absorption area, strengthen the resistance of host plant.The existence of rhizospheric microorganism also significantly improves the chlorophyll content in leaf blades of plant, thus promotes that it photosyntheticly carries out.Nitrogen element is one of chlorophyllous important component of plant materials, and the important pigment that chlorophyll is higher plant to carry out required for photosynthesis, so the total nitrogen content in plant materials and chlorophyll content directly affect the formation of plant photosynthetic rate and photosynthate, most important to the growth of plant.
In recent years, the value of selenium element is subject to everybody extensive accreditation, and supply Se scientific is also more suggested.
Selenium and the research of life entity relation prove; selenium is the important component of Selenoperoxidase; cytolemma, the important protection component of cell walls; lack its cells survival and do not reach the life-span that should survive; vegeto-animal vitality reduces greatly; immunizing power and resistance deep fades, its life quality and quality are difficult to improve.
But blueberry growth on the spot requires it is strongly-acid, P in soil H 4.0-4.5, selenium can be formed fixing by 6 valency iron at acid soil and not be absorbed and used by plants, and cause soil selenium-containing or external source to mend selenium and all can not be absorbed by blueberry, formation is serious lacks selenium state.
Present blueberry plantation great majority are all at use ammonium sulfate, potassium sulfate compound fertilizer, and nitrogen, phosphorus, potash fertilizer proportioning use, and fertilizer ratio is probably tending towards 1:1:1, is mostly for plant supplements macroelement.And most selenium element adds with Sodium Selenite to be fitted in fertilizer in the existing biological selenium fertilizer commercially sold, and Sodium Selenite is inorganic selenium, be administered to after in acid soil that blueberry plant cannot normal absorption.Blueberry is to fertilize very hard, and the blueberry juice of organic fertilizer is many and sweet.So the research and development of blueberry selenium-enriched organic fertilizer material are extremely important.
The invention belongs to a kind of activation blueberry rhizospheric microorganism special nutritional liquid and preparation method thereof and application, use microbial technique fundamentally to strengthen the absorption of blueberry to various nutrition, especially strengthen supplementing trace element.
Summary of the invention
In order to the acid soil solving blueberry growth can not the contradiction of rich selenium, the object of this invention is to provide a kind of green, environmental protection, efficiently special root fungus activator of the rich selenium of blueberry and preparation method thereof, consumption of the present invention is little, easy to use, production technique is simple, with low cost, and this product is environmentally safe, useful and harmless to blueberry growth itself.
The present invention adopts following technical scheme to achieve these goals:
A kind of activation blueberry rhizospheric microorganism special nutritional liquid and preparation method thereof, it is characterized in that: comprise the following steps: (1) first plants Herba Astragali Melilotoidis (Herba Astragali Sinici), okra, corn Tanaka, rich selenium pig manure barnyard manure is applied to Tanaka, the mu amount of executing 2400-2600kg, organic selenium-rich seed maturation gathered in the crops mixes by following per-cent: Chinese Milk Vetch Seeds about 40%, okra seed about 30%, corn seed about 30%;
(2) by the seed 0.2-0.5% water-soluble activation selenium Steeping and budding of above-mentioned mixing, make it to sprout, carry out cryodrying dehydration when bud grows to 2-5cm, make its moisture≤14%, then abrasive dust, obtain organic selenium-rich germ meal;
(3) raw material that weight part gets preparation selenium source substratum is pressed down: ecological natural pond liquid 28-32, animal blood 18-22, ecological chicken manure 6-8, starch 32-34, organic selenium-rich germ meal 4-6, potassium sulfate 3.3-3.8, phosphoric acid or potassium primary phosphate 1.4-1.6; Above-mentioned raw materials is fully mixed, is mixed with selenium source substratum;
(4) mixed strains selenium source culture medium inoculated is made up of mixed yeast, milk-acid bacteria and Bacillus subtilus, methanobacteria, ferment at 25 DEG C-34 DEG C in dark condition, the water being equivalent to substratum weight about 50% is added again after about 15 days, after being fully uniformly mixed with stirrer, filtering separation filtrate, the blueberry made of filtrate the being rich selenium root fungus special activator obtained, pack with light resistant container respectively and is placed in shady and cool ambient storage and namely can be used for the filling of blueberry rhizosphere and execute.
A kind of activation blueberry rhizospheric microorganism special nutritional liquid and preparation method thereof, is characterized in that: the weight of selenium source preparation substratum is: ecological natural pond liquid 30, animal blood 20, ecological chicken manure 7, starch 33, organic selenium-rich germ meal 5, potassium sulfate 3.5, phosphatase 11 .5; Described animal blood does not add coagulant, and described ecological chicken manure is not containing microbiotic and hormone.
Described one activates blueberry rhizospheric microorganism special nutritional liquid and preparation method thereof, it is characterized in that: described mixed yeast refers to the mixing of one or more in yellow yeast, brown yeast, white yeast and black yeast.
Described one activates blueberry rhizospheric microorganism special nutritional liquid and preparation method thereof, it is characterized in that: every 100 parts of selenium source culture medium inoculated 1-1.5 part mixed strainss in step (4).
Described one activates blueberry rhizospheric microorganism special nutritional liquid and preparation method thereof, it is characterized in that: rich selenium pig manure barnyard manure generation method is: fed pigs as green fodder by the fresh grass of plant Herba Astragali Melilotoidis (Herba Astragali Sinici) of plantation Efficient Conversion water soluble selenium, the movement of the pig obtained and vinasse, large chaff shell, rural area dry ground mix, and generate with soil and pigsty environmental microorganism spontaneous fermentation 10-12 month through artificial mixed turning over;
The implantation methods of the fresh grass of plant Herba Astragali Melilotoidis (Herba Astragali Sinici) of described Efficient Conversion water soluble selenium is: compound concentration is that the sodium selenite solution of about 100mg/L sprays application in Herba Astragali Melilotoidis (Herba Astragali Sinici), and the consumption using rich selenium product is 400kg/hm
2left and right; The movement of described pig and vinasse, large chaff shell, rural area dry ground mix according to following weight proportioning: 1:20-22:20-22:8-10.
Described one activates blueberry rhizospheric microorganism special nutritional liquid and preparation method thereof, it is characterized in that: described rich selenium pig manure barnyard manure selenium content reaches 750-790 μ g/kg.
Described one activates blueberry rhizospheric microorganism special nutritional liquid and preparation method thereof, it is characterized in that: the fresh careless selenium content of gained Herba Astragali Melilotoidis (Herba Astragali Sinici) reaches about 4.403mg/kg, and wherein organoselenium accounts for about 77%.
The present invention utilizes plant seed Herba Astragali Melilotoidis (Herba Astragali Sinici) 40%, okra seed 30%, the corn seed 30% with selenium-enriched ability, selenium-enriching cultivation is carried out in the parent cultivation of the above-mentioned seed of each leisure acquisition, obtains in seed and reach more than 70%-80% containing organoselenium.Again by these seeds 0.2-0.5% water-soluble activation selenium Steeping and budding, make it to sprout, bud grows to 2-5cm and carries out cryodrying dehydration, makes its moisture≤14%, then grinding is become face, makes finished product organic selenium content nearly 100%, selenium content >=2000mg/kg.
Substratum is prepared as selenium source with this organic selenium-rich germ meal, its ratio is: ecological natural pond liquid 30%, animal blood 20% (not adding coagulant), ecological chicken manure (not containing microbiotic and hormone) 7%, starch 33%, rich selenium germ meal 5%, potassium sulfate 3.5%, phosphatase 11 .5%, total amount 100%, and inoculate mixed yeast (yellow yeast, brown yeast, white yeast and black yeast etc.), milk-acid bacteria and Bacillus subtilus, methanobacteria (natural pond liquid exists) through abundant mixing.Anaerobism is carried out and aerobic dark condition bottom fermentation adds water 50% after about 15 days again under 25 DEG C of-34 DEG C of conditions, fully be uniformly mixed the separation of rear filter residue liquid with stirrer and make the directed root fungus activator of the rich selenium of blueberry, bacterial strain >=500-800 ten thousand strains/ml, organoselenium >=10mg/L, pH4.0-5.0.Pack with light resistant container and be placed in shady and cool ambient storage.
This nutritive medium main nutrient elements is as follows containing scale:
Beneficial effect of the present invention:
The present invention passes through microbial technique, by with plumule organoselenium (containing the organoselenium >=2000mg/kg) powder cultivated by the Chinese Milk Vetch Seeds of rich selenium, okra and corn, add in fermention medium, then, in substratum, concentrate the multiple probiotic bacteriums such as methanobacteria, mixed yeast (having yellow yeast and yeast, in vain yeast and black yeast etc.), milk-acid bacteria and Bacillus subtilus.By simulated soil alternating temperature anaerobism and aerobic fermentation, generate the active microbial inoculum of PH4.0-4.5, rich selenium methanobacteria, mixed yeast and Bacillus subtilus, bacterial strain >=500-800 ten thousand strains/ml, by 45L/ hectare, drip and execute in blueberry rhizosphere scope.Activate blueberry rhizosphere scope 5-25cm soil layer, root system generates symbiosis mycorrhiza in a large number, thus blueberry Root Absorption is strengthened, Soil Nutrient Transformation accelerates, and plant resistance strengthens, with luxuriant foliage and spreading branches in leafy profusion, fruitful branch is sturdy, fruit-setting rate is high, and fruit obviously increases, and shedding is less, fruit sugar improves, mouthfeel becomes good, and quality comparison illumination is aobvious becomes excellent, and total SOD content of fruit promotes general 16% than contrast content.Use through blueberry field its main nutrient composition and root fungus quantity with compare change obvious, microbial population wherein to like acidity, as white yeast, yellow yeast, Mucor etc.;
SOD content detection: the mensuration of Blueberry SOD adopts the SOD test kit (surveying total) being built up Bioengineering Research Institute's production by Nanjing, and article No.: A001-1, by specification detects.Because blueberry pericarp is intense violet color, compare without distilled water, but added sample liquid again after adding developer, thus elimination blueberry pericarp is the interference that intense violet color brings, total number born vigor in Accurate Determining Blueberry.
Use its main nutrient composition and root fungus quantity through blueberry field and compare and change obviously, and total SOD content of fruit promotes about 16% than contrast content.This mycorhiza activator is easy to use, and production technique is simple, does not need specific installation, with low cost, environmentally safe, overcomes the defect that blueberry under acid soil cannot absorb selenium, for blueberry plantation fertilising opens new way.
Subordinate list illustrates:
To be an experimental plot the present invention on blueberry root fungus formed table 1 and the impact of fruit quality
Table 2 is that No. two experimental plots are used the present invention and formed and the impact of fruit quality blueberry mycorhiza
Embodiment
A kind of activation blueberry rhizospheric microorganism special nutritional liquid and preparation method thereof, comprises the following steps:
(1) first Herba Astragali Melilotoidis (Herba Astragali Sinici), okra, corn is planted Tanaka, rich selenium pig manure barnyard manure is applied to Tanaka, the mu amount of executing 2500kg, organic selenium-rich seed maturation gathered in the crops mixes by following per-cent: Chinese Milk Vetch Seeds 40%, okra seed 30%, corn seed 30%;
(2) by the seed of above-mentioned mixing 0.3% water-soluble activation selenium Steeping and budding, make it to sprout, carry out cryodrying dehydration when bud grows to 3cm, make its moisture≤14%, then abrasive dust, obtain organic selenium-rich germ meal;
(3) raw material that weight part gets preparation selenium source substratum is pressed down: ecological natural pond liquid 30, animal blood 20, ecological chicken manure 7, starch 33, organic selenium-rich germ meal 5, potassium sulfate 3.5, phosphatase 11 .5; Described animal blood does not add coagulant, and described ecological chicken manure is not containing microbiotic and hormone; Above-mentioned raw materials is fully mixed, is mixed with selenium source substratum;
(4) mixed strains selenium source culture medium inoculated is made up of mixed yeast, milk-acid bacteria and Bacillus subtilus, methanobacteria, every 100 parts of selenium source culture medium inoculateds 1 part of mixed strains, ferment at 30 DEG C in dark condition, the water being equivalent to substratum weight 50% is added again after 15 days, after being fully uniformly mixed with stirrer, filtering separation filtrate, the blueberry made of filtrate the being rich selenium root fungus special activator obtained, pack with light resistant container respectively and is placed in shady and cool ambient storage and namely can be used for the filling of blueberry rhizosphere and execute.
Described mixed yeast refers to the mixing of one or more in yellow yeast, brown yeast, white yeast and black yeast.Rich selenium pig manure barnyard manure generation method is: fed pigs as green fodder by the fresh grass of plant Herba Astragali Melilotoidis (Herba Astragali Sinici) of plantation Efficient Conversion water soluble selenium, the movement of the pig obtained and vinasse, large chaff shell, rural area dry ground mix, and the movement of described pig and vinasse, large chaff shell, rural area dry ground mix according to following weight proportioning: 1:20:20:8; Within 12 months, generate with soil and the spontaneous fermentation of pigsty environmental microorganism through artificial mixed turning over;
The implantation methods of the fresh grass of plant Herba Astragali Melilotoidis (Herba Astragali Sinici) of described Efficient Conversion water soluble selenium is: compound concentration is that the sodium selenite solution of 100mg/L sprays application in Herba Astragali Melilotoidis (Herba Astragali Sinici), and the consumption using rich selenium product is 400kg/hm
2; The fresh careless selenium content of gained Herba Astragali Melilotoidis (Herba Astragali Sinici) reaches 4.403mg/kg, and wherein organoselenium accounts for 77%;
Described rich selenium pig manure barnyard manure selenium content reaches 750-790 μ g/kg.
Rhizosphere 5-25cm soil layer scope is executed below by being dripped by drip irrigation integrated device by the liquid root fungus activator of the present embodiment gained in two pieces of experimental plots; Every mu is 3kg by the present embodiment amount.
Case 1: number blueberry experimental plot.
Soil characteristic: pH5.5-5.8, clay, organic 1.56%, alkali-hydrolyzable nitrogen 127.07mg/kg,
Available phosphorus 7.85mg/kg, available potassium 138.66mg/kg.
Table 1: root fungus is activated special nutrient fluid and formed blueberry mycorhiza and fruit quality impact
Case 2: No. two experimental plots.
Soil characteristic: pH4.7-5.0, reddish yellow gravel grain soil.Organic 0.83%, alkali-hydrolyzable nitrogen 102.56mg/kg, available phosphorus 3.32mg/kg, available potassium 98.75mg/kg.
Table 2: root fungus is activated special nutrient fluid and formed blueberry mycorhiza and fruit quality impact
Claims (7)
1. activate a blueberry rhizospheric microorganism special nutritional liquid and preparation method thereof, it is characterized in that: comprise the following steps:
(1) first Herba Astragali Melilotoidis (Herba Astragali Sinici), okra, corn is planted Tanaka; rich selenium pig manure barnyard manure is applied to Tanaka; the mu amount of executing 2400-2600kg, organic selenium-rich seed maturation gathered in the crops mixes by following per-cent: Chinese Milk Vetch Seeds about 40%, okra seed about 30%, corn seed about 30%;
(2) by the seed 0.2-0.5% water-soluble activation selenium Steeping and budding of above-mentioned mixing, make it to sprout, carry out cryodrying dehydration when bud grows to 2-5cm, make its moisture≤14%, then abrasive dust, obtain organic selenium-rich germ meal;
(3) raw material that weight part gets preparation selenium source substratum is pressed down: ecological natural pond liquid 28-32, animal blood 18-22, ecological chicken manure 6-8, starch 32-34, organic selenium-rich germ meal 4-6, potassium sulfate 3.3-3.8, phosphoric acid or potassium primary phosphate 1.4-1.6; Above-mentioned raw materials is fully mixed, is mixed with selenium source substratum;
(4) mixed strains selenium source culture medium inoculated is made up of mixed yeast, milk-acid bacteria and Bacillus subtilus, methanobacteria, ferment at 25 DEG C-34 DEG C in dark condition, the water being equivalent to substratum weight about 50% is added again after about 15 days, after being fully uniformly mixed with stirrer, filtering separation filtrate, the blueberry made of filtrate the being rich selenium root fungus special activator obtained, pack with light resistant container respectively and is placed in shady and cool ambient storage and namely can be used for the filling of blueberry rhizosphere and execute.
2. one according to claim 1 activates blueberry rhizospheric microorganism special nutritional liquid and preparation method thereof, it is characterized in that: the weight of selenium source preparation substratum is: ecological natural pond liquid 30, animal blood 20, ecological chicken manure 7, starch 33, organic selenium-rich germ meal 5, potassium sulfate 3.5, phosphatase 11 .5; Described animal blood does not add coagulant, and described ecological chicken manure is not containing microbiotic and hormone.
3. one according to claim 1 activates blueberry rhizospheric microorganism special nutritional liquid and preparation method thereof, it is characterized in that: described mixed yeast refers to the mixing of one or more in yellow yeast, brown yeast, white yeast and black yeast.
4. one according to claim 1 activates blueberry rhizospheric microorganism special nutritional liquid and preparation method thereof, it is characterized in that: every 100 parts of selenium source culture medium inoculated 1-1.5 part mixed strainss in step (4).
5. one according to claim 1 activates blueberry rhizospheric microorganism special nutritional liquid and preparation method thereof, it is characterized in that: rich selenium pig manure barnyard manure generation method is: fed pigs as green fodder by the fresh grass of plant Herba Astragali Melilotoidis (Herba Astragali Sinici) of plantation Efficient Conversion water soluble selenium, the movement of the pig obtained and vinasse, large chaff shell, rural area dry ground mix, and generate with soil and pigsty environmental microorganism spontaneous fermentation 10-12 month through artificial mixed turning over;
The implantation methods of the fresh grass of plant Herba Astragali Melilotoidis (Herba Astragali Sinici) of described Efficient Conversion water soluble selenium is: compound concentration is that the sodium selenite solution of about 100mg/L sprays application in Herba Astragali Melilotoidis (Herba Astragali Sinici), and the consumption using rich selenium product is 400kg/hm
2left and right; The movement of described pig and vinasse, large chaff shell, rural area dry ground mix according to following weight proportioning: 1:20-22:20-22:8-10.
6. one according to claim 1 activates blueberry rhizospheric microorganism special nutritional liquid and preparation method thereof, it is characterized in that: described rich selenium pig manure barnyard manure selenium content reaches 750-790 μ g/kg.
7. one according to claim 5 activates blueberry rhizospheric microorganism special nutritional liquid and preparation method thereof, it is characterized in that: the fresh careless selenium content of gained Herba Astragali Melilotoidis (Herba Astragali Sinici) reaches about 4.403mg/kg, and wherein organoselenium accounts for about 77%.
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| CN107223859A (en) * | 2017-06-28 | 2017-10-03 | 四川硒精康生物科技有限公司 | A kind of inorganic selenium conversion process fermented by beneficial bacterium |
| CN108841496A (en) * | 2018-07-21 | 2018-11-20 | 刘小平 | Brewed wine containing Chinese Milk Vetch Seeds in a kind of raw material |
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Application publication date: 20150513 |