KR20050032050A - Culture method of chlorella containing selenium and germanium - Google Patents

Abstract

A method for culturing Chlorella containing selenium and germanium is provided, which Chlorella has anticancer and antioxidative effects derived from selenium and germanium, and has no toxicity, so that the Chlorella can be useful in health food and animal feed. The method for producing Chlorella containing selenium and germanium comprises culturing Chlorella using inorganic Na2SeO3, Na2SeO4, selenium-yeast complex, organic selenium fermented by a microorganism, organic selenium chelated with various proteins and vitamins and selenium containing material; and inonized Ge02, Ge132, germanium-yeast complex, germanium containing material chelated with vitamine, and germanium containing material fermented by a microorganism.

Description

Cultivation method of chlorella containing selenium and germanium {omitted}

The present invention relates to a method of culturing chlorella containing both organic selenium and germanium. The chlorella cultivated according to the present invention contains organic selenium and germanium, which are not toxic to the human body and have high absorption rate, which is a natural healing substance lacking in modern people. Known functions of selenium and germanium, such as antioxidant power, improve heart and lung function, breakdown of waste materials, prevent cirrhosis, improve immune function, improve sexual function, treat muscle nutrition and prevent cancer.

Recently, efforts have been made to increase nutritional added value by adding desired nutrients, elements or substances to existing agricultural products. A typical example is the supply of organic or inorganic selenium and germanium as fertilizers to plants, and after the organic process through the plants once again concentrated in the plant to the effective concentrations of selenium and germanium known to the human body, anti-cancer and antioxidant components There are also attempts to accumulate certain useful ingredients in livestock products such as meat and oil.

Selenium is an element belonging to group 6B of the chemical periodic table and is contained in abundance in atomic number 34, element symbol Se, brewer's yeast of atomic weight 78.96, pork and beef kidney, butter, infant malt, oyster, shrimp, milk, brown rice and garlic. In animals such as humans, they are mainly present in the form of seleno amino acids such as selenocysteine and selenomethionine, and they activate glutathione peroxidase, which acts to remove hydrogen peroxide, which causes damage to the cell membrane. It is known to have the effect of preventing tumoral disease by preventing. In many studies, selenium has been shown to have an inhibitory effect on tumor disease, which is thought to be associated with abnormally low serum levels of selenium in many cancer patients. In addition, anemia caused by lead poisoning is a hematopoietic effect by increasing the excretion of the urinary tract of aminolevulinic acid by inhibiting the activity of S-aminolevulinate dehydrase (ALAD) enzyme, which is involved in hemolytic action of hemoglobin and hemoglobin synthesis. In addition to inhibiting, it is known that lead is caused by inactivation of the SH group of ALAD and deterioration of hematopoietic function. It has been reported that lead poisoning can also be detoxified by selenium, which is known as an antioxidant in vivo, because glutathione peroxidase, a selenium-dependent antioxidant enzyme whose activity is weakened by lead, is significantly increased by selenium supplementation. It is thought to be. This is because, when lead poisoning in rats is added to the diet of selenium at a concentration of 0.5 ppm, hepatic tissue peroxidation damage is reduced as compared to the non-added group. This lead poisoning detoxification of selenium is explained because the SH group of ALAD is replaced with SeH, thereby preventing the inactivation of SH group by lead. Other similar mechanisms have reported that selenium is effective in the detoxification of heavy metals such as cadmium and mercury in addition to lead. A summary of the effects of selenium other than these are as follows:

The antioxidant power of selenium is 1975 times that of tocopherol, and synergistic effect occurs when tocopherol and selenium are used together.

· It has a therapeutic effect on dandruff and psoriasis and increases the utilization of oxygen to improve heart and lung function.

It inhibits the oxidation of unsaturated fatty acids such as vitamins C and E, which not only suppresses the production of lipid peroxide, but also decomposes waste materials that are a combination of lipid peroxide and protein.

It combines with heavy metals such as mercury, cadmium and lead to inactivate them and prevents necrosis of liver cells to prevent cirrhosis.

Improves immune function and a significant amount (approximately 25-40%) is roofed in male sex organs such as testicles, seminal vesicles and prostate gland to improve sexual function.

Prevents cataracts and rheumatoid arthritis and synergizes with vitamin E. Muscle is effective for muscular dystrophy.

Delays aging of the human body, prevents cancer and improves lung and heart function

Germanium is one of the carbon group elements belonging to group 4B of the periodic table. It is known as the elemental symbol Ge, atomic number 32, and oxygen for which the atomic weight is 72.59%.

High-purity organic germanium completely escapes from the human body through urine without holding and accumulating cancer cells, toxins, contaminants, and heavy metals in the human body within about 20-30 hours after taking various tasks in the human body after taking them. It is very safe because there is no toxicity at all.

Source: Asai Germanium Research Institute, Tokyo, Japan.

On the 28th and 6th month of the animal experiment, 1mg / kg / Day was administered and the result of close observation showed no special toxicity or poisoning phenomenon except for the slight loss of weight in the male at the end of 6 months. In addition, because germanium is excreted in the urine as it is taken, there were no symptoms of accumulation or poisoning in any organ after 6 months of testing.

Source: Journal de Toxicologie Clinique et Experimental Dec. 11, 1991 (7-8) 421-36-

Organo germanium (Ge-132) prevents injury to cells

Germanium (Ge-132) has been found in animal studies of the effects on injured cells to prevent self-treatment and prevent the injury of cells in animals given Germanium (Ge-132). All these effects are related to the role of germanium (Ce-132). These results show that germanium (Ge-132) promotes smooth metabolism of cells, which is protected from injury.

Source: Yao Hsueh Hsueh Pao Acta Pharmaceutica Sinica, 1992, 27 (7): 481-5

Germanium as an Individual to Alleviate Chronic Fatigue (Ge-132)

Germanium has been found to attenuate and alleviate the symptoms of the Chronic Epstein-Barr Virus Syndrome. Millions of Americans, as well as many people around the world, suffer from this chronic Epstein-Barr Virus Syndrome.

Source: Use of Organic Germanium in Chronic Epstein-Barr Virus Syndrome, 'Journal of Orthomolecular Medicine, 1989

Germanium slows the development of cataracts, Ge-132 slows the development of cataracts, and has been shown to be effective in the prevention and treatment of cataracts.

Source: Department of Biological Sciences, Oakland University,

Rochester, MI, Experimental Eye Research, August 1995. '

Germanium as an inhibitor of mutagenic factor

Many studies and papers recommend a balanced diet of several essential nutrients. In particular, nutrients such as antioxidants, vitamins and selenium are potential and definitive carcinogen inhibitors. Furthermore, constant doses of germanium have been shown to inhibit mutagenic factors.

Source: Department of Oral Biology, Indiana University School of Dentistry, Indianapolis 46202.Mutation Research 1995 Aug., 335 (1): 21-6

Germanium improves postoperative recovery and immunity

Germanium has been shown to be effective as an interferon derivative and to enhance immunological response to rejection of post-operative phagocytes, leukocyte chromatin, lymphocytes around blood, and their control groups. Such effects of germanium persisted for a long time after surgery, and these results suggest that germanium is effective as a prophylactic and therapeutic agent for several clinical symptoms of postoperative immune rejection in animals. It is shown.

Source: Journal of Veterinary Medical Science, 1993 Oct., 55 (5): 795-9.

Effect of Germanium on Cancer and Malignant Tumors

Many research papers and clinical reports from the 16th and 19th centuries have mentioned that metals and metal compounds are useful in the treatment of cancer and leukemia. Unfortunately, the fact that malignant tumors, cancers, etc. can be conquered by inorganic and metal compounds, that is, such studies were forgotten by people until 1960. Many metal compounds, including germanium, have shown great effects as anticancer and malignant tumor removers in animal and human clinical trials. Source: European Journal of Clinical Pharmacology, 1994, 47 ( 1): 1-16

In animal experiments, organic germanium has a lot to do with blood pressure-lowering effects, and the results of these studies on the blood pressure-lowering effects of organic germanium are found in page 112 of the 38th annual meeting of the 38th Annual Meeting in Tokyo, Japan. Source: The Clinical Report (Kiso to Rinsho, in Japanese) 7 (1978): 719

Organic germanium has been considered to restore the immune system to its original state in older mice that have lost their immune response.

Source: International Archives of Allergy 63 (1980): 338-339

Germanium has been shown to be a potent potent inhibitor of mutagenesis in Salmonella cocci, and its effects on bacteria have been noted. Source: Mutation Research 125 (1984): 145-151

Organic germanium has been shown to reduce osteoporosis as well as bone fracture in osteoporosis patients.

Source: Asai Germanium Research Institute, Tokyo, Japan, 1984.

Oral administration of germanium has the positive effect that the interferon inducer is regulated in the body.

Source: Microbiology and Immunology 29, 1985: 65-74.

Germanium oxide (organic germanium) has been shown to act as an important interferon derivative in the human body. Source: Proceedings of the Japan Cancer Association II, Annual Meeting, 1979, Pagel 93.

Organic germanium has been shown to inhibit tumors, malignant tumors.

Source: Cancer and Chemotherapy 6, in Japanese, (1968): 79-83.

Oral administration of organic germanium has been observed to significantly prolong life. Source: Gan To Kagaku Ryoho 12, December 1985: 2345-51.

The fact that organic germanium is a potent tumor and malignant tumor suppressor has been shown to be related to the function of organic germanium as an interferon derivative.

Source: Tohoku Journal of Experimental Medicine 146, May 1985: 97

In animal experiments on tumor suppression, tumors stopped growing in animals administered with organic germanium and the tumors soon contracted.

Source: Anticancer Research 6, March-April 1986: 177-82.

Animal experiments on the therapeutic effects of organic germanium have been shown to prevent tumor growth. These phenomena appear to be the result of the active activity of the phagocytic cells in the body. (The phagocytes are also one immune system, they attack the enemy)

Source: Gan To Kagaku Ryoho 12 November 1985: 2122-8.

We have studied the results of organic germanium when used with chemotherapy, an anticancer drug, as a tumor suppressor. The results showed that tumor growth stopped, tumor metastasis was inhibited, life span was prolonged, the side effects of chemotherapy were reduced, and the weight lost to chemotherapy was restored.

Source: Gan To Kagaku Ryoho 13, Aug. 1986: 2588-93.

Animals inoculated with cancer tumors and leukemia cells were treated with organic germanium. In this study, organic germanium has been shown to be effective in improving the body's defenses rather than directly attacking cancer cells. In other words, the immune system including phagocytic cells and T-cells seems to work. Source: Anticancer Research 5, Sep.-Oct. 1985: 479-83

In animal experiments against changes in blood pressure, normal blood pressure was not changed as observed after germanium administration to animals with normal blood pressure. However, after administration to animals with high blood pressure, the blood pressure was surprisingly returned to normal 7-10 days after Germanium administration. This effect persisted for quite some time after stopping the administration of germanium.

Source: Japan Experimental Research Institute, Tokyo, Japan.

Organic germanium has shown the same strong analgesic effect as morphine in oral and injection. Taking or administering germanium has been shown to increase the production of endorphins in the body and to increase the analgesic effects of each organ of the body.

Source: Journal of Pharmacological Dynamics 6 (1983): 814-820.

Organic germanium with high purity is completely escaped from the human body through urine without holding and accumulating cancer cells, toxins, pollutants and heavy metals in the human body within about 20-30 hours after completing various tasks in the human body after taking. It is very safe because there is no toxicity at all.

Source: Asai Germanium Research Institute, Tokyo, Japan.

In the present invention, in the case of selenium, chlorella containing selenium can be incubated with Na 2 SeO 3, Na 2 SeO 4, selenium yeast complex, organic selenium fermented with microorganisms, and organic selenium chelated with various proteins or vitamins.

In case of germanium, germanium-containing chlorella can be cultured using ionized GeO2, Ge132, germanium-containing material chelated with vitamins and proteins, and germanium-containing material fermented with microorganisms.

In addition, the above method can be mixed to incubate chlorella containing both selenium and germanium.

In the present invention, Na2SeO3, Na2SeO4, selenium yeast complex, organic selenium fermented with microorganisms, organic selenium chelated with various proteins or vitamins, and ionized GeO2, Ge132 and germanium yeast complexes, germanium-containing substances chelated with vitamins and the like When culturing chlorella by mixing together germanium-containing materials fermented with microorganisms, it is successful to cultivate safe chlorella with high absorption rate and high availability of selenium and germanium at the same time by adding and mixing appropriate amounts of selenium and germanium solution in water. To complete the present invention.

Selecting one of Na 2 SeO 3, Na 2 SeO 4, selenium yeast complex, organic selenium fermented with microorganisms, and organic selenium chelated with various proteins or vitamins;

Selecting one of ionized GeO 2, Ge132 and germanium yeast complexes, germanium-containing material chelated with vitamins, and germanium-containing material fermented with microorganisms;

-Appropriately formulating and mixing a culture solution that becomes a nutrient for chlorella, (glycosase, nitrogen, ammonia, amino acids, glucose, fructose, maltose, selenium, germanium, etc.);

Culturing chlorella with chlorella and broth in a culture vessel having good sunlight and temperature conditions of 25 to 30 ° C., oxygen supply, and rotation of water; It is composed of the steps of recovering the well-cultivated chlorella and completed the present invention by completing a chlorella containing both a safe and good availability of organic selenium and germanium at the same time

Hereinafter, although an Example and a test example demonstrate this invention, the scope of the present invention is not restrict | limited by these.

Hereinafter, the present invention will be described in detail with reference to Examples.

Example

In the present invention, Na 2 SeO 3, Na 2 SeO 4, selenium yeast complex, organic selenium fermented with microorganisms, and organic selenium chelated with various proteins or vitamins may be used, but preferably Na 2 SeO 3 is selected and used.

For germanium, germanium-containing materials chelated with inorganic ionized GeO2, Ge132 and germanium yeast complexes, vitamins, and germanium-containing materials fermented with microorganisms can be used, but for germanium, Ge132 was selected.

Ionized the selected selenium and germanium, and then mixed selenium (0.1ppm) and germanium (0.0lppm) on the basis of 1 ton of the culture solution composed of chlorella nutrients. Using the air purifier registered (Registration No .: 03l879l), supplying purified oxygen in a sterile state while rotating the water using a rotating shaft to illuminate the chlorella while helping to chlorella's photosynthesis, and then using a centrifuge Chlorella was isolated to recover the precipitated chlorella, and 0.8 mg / kg of selenium and 0.05 mg / kg of germanium were detected in the cultured chlorella by adding a selenium and germanium solution. Chlorella contains protein, chlorophyll, carbohydrates, minerals and vitamins, but it is superior to other plants in terms of quality and quantity. Chlorella contains special substances such as C, G, and F (growth growth factors), which are foods that overcome the pollution.

The table below is a nutritional analysis table of chlorella, and the present invention includes chlorella with inorganic nutrients selenium and germanium.

The present invention was completed by completing a safe chlorella having high human absorption rate and good usability.

Chlorella cultured according to the present invention contains a large amount of vegetable organic selenium and vegetable organic germanium which have anti-cancer and antioxidant effects, so that it is not toxic and has a therapeutic and preventive effect. Selenium and germanium are naturally available.

Claims (3)

According to claim 1, the present invention is an inorganic Na 2 SeO 3, Na 2 Se 04, selenium yeast complex, organic selenium fermented with microorganisms, organic selenium chelated with various proteins or vitamins, and selenium-containing chlorella cultured using a selenium-containing material Culture method of The method of culturing germanium-containing chlorella according to claim 2, wherein the germanium-containing chlorella is cultured using a germanium-containing material chelated with ionized GeO2, Ge132 and germanium yeast complexes, vitamins, and the like. 4. The method according to claim 3, which is chelated with inorganic Na 2 SeO 3, Na 2 SeO 4, selenium yeast complex, organic selenium fermented with microorganisms, organic selenium chelated with various proteins or vitamins, and ionized GeO 2, Ge132 and germanium yeast complexes, vitamins, and the like. A method of culturing chlorella containing both selenium and germanium, which are cultured using germanium-containing materials and germanium-containing materials fermented with microorganisms.