GB2214928A - Processing for preparing algae having improved biological effects - Google Patents
Processing for preparing algae having improved biological effects Download PDFInfo
- Publication number
- GB2214928A GB2214928A GB8806532A GB8806532A GB2214928A GB 2214928 A GB2214928 A GB 2214928A GB 8806532 A GB8806532 A GB 8806532A GB 8806532 A GB8806532 A GB 8806532A GB 2214928 A GB2214928 A GB 2214928A
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- Prior art keywords
- alga
- strain
- selenium
- culture medium
- algae
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/60—Edible seaweed
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
Description
p 1 -, "' 1 A. 9 2 8 z L 1 PROCESS FOR PREPARING ALGAE HAVING IMPROVED
BIOLOGICAL EFFECTS This invention relates to a process for preparing algae having improved biological effects.
Algae have from time immemorial been used by the mankind for the purpose of nutrition and feeding. Thus, algae are mainly consumed by the peoples of the Far East; recently, however, they are utilized in dry form or in the form of tablets in the developed countries, too. Algae are the carriers of highly valuable nutritive materials since their dried form contains high concentrations of substances which are essential for a healthy life such as vitamins, proteins, complexes of protein rricroelemen"t,s, saccharides, polyunsaturated fatty acids and the like.
In recent years, the environmental pollution became a worldwide problem. Today, the contamination of seas and oceans by toxic heavy metals(e.g. lead, mercury, cadmium, copper) as well as tumour-inducing agents (e.g. condensed polycyclic aromatic compounds) is not negligible. It is a difficult problem that these agents are accumulated in the organism of the algae. Thus, "complete algae" obtained from the sea or cultivated under other natural conditions cannot be used for the purposes of human or animal nutrition,'feeding, cosmetics and therapy; only such fractions of the 99 complete algae" can be used which have previously been purified of the biologically harmful materials. A purifying process of this type is described e.g. by Carames de Guova /-Cosmetics and Toiletries 95, 47 (1980)7. However, a purifying operation means an intervention decomposing a part of the biologically active substances of the alga whereby the biological value of the thus-obtained alga fraction is significantly decreased /._Zajic: Properties and Products of Algae: Edition Planum, New York, (197027.
As a consequence, the preparation of sterile algae being free from environmental damages is becoming more and more important.
A number of processes are known for the artificial cultivation of algae which may be performed e.g. in an open basin under sunlight or in a closed space under conditions ensuring sterility either under natural or artificial illumination, or by exclusion of any light.
According to the published Japanese patent application No. 56.96690 algae are cultivated by using maritime alga strains in sterilized sea-water, in a solution containing various nutritive salts under artificial light source.
According to the published Japanese patent application No. 45.17146 the industrial cultivation of unicellular green Chlorella algae is carried out on a sterile fresh-water culture medium under the exclusion of carbon dioxide and light.
The cultivation of unicellular fresh-water green algae is also disclosed in the French patent specification No.
tw 1 11 - 3 9 2,103,462 according to which the industrial-scale cultivation is photosynthetically realized by the use of nutritive solu tions containing appropriate nutrient salts.
According to the published Hungarian patent applications Nos. 4613/84 and 4614/84 the cultivation of algae is accomplished in a mineral water of natural origin, medicinal or thermal water or in a mixture thereof enriched by a metal compound up to a concentration of 10- 2 mole/litre.
In any of the known solutions, algae to be used for manufacturing food, fodder or medicines are artificially produced under sterile conditions. In these processes, algae are cultivated under conditions resulting in properties of the thus-obtained algae similar to those cf- algae spontaneously growing in the nature under harmless environmental conditions; or the properties of the thus-produced' algae are different only to a low extent, depenting on tie marnner cf the artificial cultivation.
The algae artificially cultivated by using the processes of the prior art contain only traces, if any, of. several elements such as selenium, zinc or silver.
It is known further that selenium has a versatile biological function as published in a comprehensive paper of Thressa et al. /-Nutrition Review 35, 7 (1977)7, Shamberger /-J. of Env. Path. and Tox. 4, 305 (1980)7 or Masukawa 'et al.
/-Experientia 39, 405 (1983)7. Thus, it is known that selenium in itself has a hypotensive effect, improves the ischaemic, hypoxic and infarction states of the heart and inhibits the ceroidal lipofuscinosis of the central nervous system; it also exerts a beneficial effect on periodcntitis and proved to diminish the probability of the development of cancer diseases; furthermore, it is considered to be a mutagen.esis-inhibiting agent. A number of alterations or diseases.
respectively, such as liver necrosis, myonecrosis, destruction of the erythrocyte membrane, interstitial laesions, ST-elevation in the ECG, kwashiorcor syndrome (protein malnutrition) and multiplex sclerosis proved to be induced by a selenium deficiency.
The beneficial action of selenium is mainly based on its activating effect directed to the glutathione-percxidase enzyme which is the most important endocenic inhibitor of the harmful peroxidation processes. Being an indispensable constituent of the prosthetic group of the glutathione -peroxidase enzyme, selenium is one of the most important and indispensable substances of life which is not accumulated in the organism and thus it has to be continuously supplied.
Until now selenium has exclusively been introduced to the organism by inorganic compounds (selenium dioxide, sodium selenite and the like).
The aim of the present invention is to provide algae having particular biological properties and, owing to their increased selenium content, capable to assert the biological effect of selenium.
The invention is based on the recognition that, under certain conditions, the alga can not only be maintained as ali,.e in selenium-containing solutions, i.e. it does not perish, but it also grows and thus it can be cultivated in a A 1 - nutritive solution containing selenium in a high concentration. This recognition is surprising since, according to the prior art, the alga was expected to be killed by the poisonous selenium.
Further on, the invention is based on the recognition that selenium is incorporated in the organism of an alga cultivated on a seleniumcontaining culture medium under certain conditions.
Thus, the invention relates to a process for the preparation of algae having improved biological effects, wherein the selected alga strain is cultivated under sterile conditions on a culture medium consisting of fresh water and nutritive salts in the presence of carbon dioxide and light in a photosynthetic way, or with the exclusion of light on a culture medium containing also a source of carbon, hydrogen, fter the th-us-obtained alga is cxygen and nitrogen, whereaj separated. According to the process of the invention, inorganic and/or organic selenium compound(s) is (are) added to the culture medium in a concentration of 107 to 2xlO_ 3 mole/litre, then the culture medium is inoculated with a pure alga culture capable to readily incorporate selenium. The pure culture is obtained by inoculating the selected alga strain onto a liquid culture medium containing inorganic and optionally organic nutritive materials and then treating it with N-methyl-NI-nitro-N-nitrosoguanidine. From the individuals of the strain thus obtained by mutation, those are selected which are capable to readily incorporate selenium when cultivated after mutation on a selenium- containing culture medium and - 6 have a growth rate identical at least to that of the wild strain. After inoculating the culture medium with the thus-obtained pure culture and after the cultivating period the alga is separated, if desired, dried to an optional water content and decomposed by supersound or grinding in a manner known per se.
According to the invention, the pure alga culture which is capable to incorporate selenium is preferably prepared from unicellular green or blue algae such as Chlorella sp., Scenedesmus sp. or Spirulina sp. in such a way that, after the treatment with N-methyl-NI-nitro-N-nitrosoguanidine, the cells are carefully washed out and dispersed onto a series of solid culture media prepared by halving dilution and supplemented with a selenium compound to a concentration between 10- 7 and 2x10- 3 mole/litre. The most preferred strains of the here developing ones having a rapid growth and capable of readily incorporating selenium are selected and further cultivated in a liquid medium, then the thus-maintained pure alga culture is subjected to industrial production.
Thus, selenium is incorporated during the growth to the organism of the alga treated as described hereinabove, in a concentration which is in average 10 4 times higher than that in the original alga strain.
Distilled water is used as liquid in the culture medium employed for the industrial cultivation of the alga strain which is capable of readily incorporating selenium. In addition to the commonly used known nutritive materials, these culture media are supplemented also with inorganic 1 and/or organic selenium compounds. The thus-prepared and sterilized culture medium is inoculated with the alga strain, obtained as described above,which is capable of readily incorporating selenium. In the course of its growth, the alga incorporates selenium to its own organism without the undesired toxic contaminations discussed above.
The thus-prepared alga is separated from the culture medium and carefully dried in a known manner under mild conditions at an optimum temperature of 65 0 C and not higher than 80 0 C. The dried alga is suitably decomposed by grinding to a particle size Of about 1 /um. Alternatively, the wet alga concentrate is decomposed by supersound and then dried under the conditions defined above.
The thus-obtained alga is a powder containing 250 to 4000 /ug/g of selenium which may directly be consu. med or used in itself or in foods, fodders, cosmetics or together with therapeutically useful and biologically active substances and/or as their additive, in the form of any commercially available formulation of these products, preferably in the form of tablets and capsules or in other forms.
The main advantages of the process according to the invention can be summarized as follows: a) The alga can be cultivated in a simple equipment by using an easily practicable cheap process.
b) After cultivation no purification or multistep working-up is needed.
c) The alga is cultivated under sterile conditions, with exclusion of environmental contaminations whereby the 8 -- i obtained alga is unambiguously usable for human consump tion.
d) It renders possible the preparation of algae with a high selenium content and possessing improved biological and physiological effects as compared to those of known algae.
e) The algae obtained by the process of the invention can preferably and widely be used, e.g. for alimentation, in the cosmetic industry, for therapeutical purposes and in other areas.
The use of the algae cultivated by the process of the invention is most preferred in the therapy since selenium is an activating agent of the prosthetic group of the glu+,atLi;ione-peroxidase enzyme. Selenium is not accumulated in the organism, so its supplementation is rendered possible with the algae obtained by the process of the invention where by all health damages can be medicated which are the consequences of selenium deficiency.
The process of the invention is illustrated in detail by the following non-limiting Examples.
Example 1
A Scenedesmus obtisiusculus culture is maintained in a 250 ml flask.in a shaken culture on Bold,s liquid culture medium containing 100 to 500 /ug/ml of N-methyl-NI-nitro-N -nitrosoGuanidine at 25 to 27 0 C for 30 minutes. After this treatment, the cells are carefully washed with water and dispersed onto a Bold's culture medium series solidified 1 t by agar. This culture medium series contains selenium in a concentration regularly increasing to a double value each from 3.125 /ug/ml to 400 /ug/ml. The colonies formed from the cells growing and surviving on this culture medium series are isolated and the selected cell lines are propagated on a laboratory scale in a Bold's nutritive solution containing at least 20 /ug/ml of selenium. Those colonies are propagated which most preferably incorporate selenium and have a growth rate nearly indentical to that of the wild (control) strain in a culture medium containing as least 20 /ug/ml of selenium.
The selenium content of the alga is determined by drying at 65 OC the cells previously isolated and carefully washed with water, decomposing them by supersound and then measuring the selenium -content by using atornabsorption method.
The selenium content of the thus-obtained alga powder is as follows:
lit r a ii n Selenium content (ug/g of alga powder) Untreated wild I strain Untreated wild II strain Code No. FM-1-120 strain Code No. FM-1-1871 strain Code No. FM-441/67 strain Code No. FM-449/E7 strain 1300 2400 1600 1800 1 f Example 2 mg of sodium selenite are added to 8 litres of Knop-Pringsheimls culture medium filled in an alga-cultivating glass bottle of 10 litres volume. The thus-obtained nutritive solution is sterilized at 121 OC under an overpressure of 1 bar for 30 minutes. Then, the sterile solution is cooled and inoculated with a pure culture of Scenedesmus obtisiusculus which is capable to readily incorporate selenium. Sterile air containing 5-'s by volume-of carbon dioxide is bubbled through the culture medium at 25 0 C while the system is illuminated by an electric discharge tube working with 4000 lux at a wavelength of 440 to 520 and 640 to 700 / UM.
After a cultivation period of 14 days the alga is separated from the culture medium, then washed with water. The thus-obtained alga mass is decomposed by supersound and carefully dried at a temperature below 65 0 C. The selenium content of the thus-obtained alga powder is 1200 / ug/g.
Example 3 ate 8.0 g of sodium nitrite, 0.8 g of magnesium sulf heptahydrate, 0.8 9 of dipotassium hydrogen phosphate, 2.5 ml of Arnon's trace element solution as well as 5 9 of glucose, 0.1 g of cysteine and 0.1 g of methionine are dissolved in 8 litres of di.stilled water filled in an alga-cultivatng fermentor of 10 litres volume. The thus-obtained nutritive solution is supplemented with 50 mg of sodium selenite, then the solution is flown through a sterilizing filter and inoculated under carefully maintained sterile conditions 7T 5% i 1 -j - 1 1 - with a pure.alga culture of Scenedesmu5 obtisiusculus capable to readily incorporate selenium. After a cultivating period of 4 days at 25 to 28 OC in the dark, the alga is separated from the culture medium and washed with water.
The thus-obtained alga mass is decomposed by supersound and finally dried at a temperature below 65 OC. The selenium content of the thus-obtained alga powder amounts to 1380 /ug/g.
Example 4
The process described in Example 2 is followed, except that instead of treatment by heat, the culture medium is sterilized by flowing through a G-5 type sterilizing bacterium filter. The selenium content of the thu5obtained alga powder is 1300 /ug/g.
Example 5
The process described in Example 2 or 3 is followed, except that instead of the Scenedesmus obtisiusculus strain, a Chlorella vulgaris alga strain is used which is capable to readily incorporate selenium and which has been subjected to the mutation treatment described in Example 1.
The selenium content of the thus-obtained alga powder is as follows:
1 S trai n Selenium content (jug/g of alga powder) "Wild" I strain "WilC II strain OV-35-42 strain DV-78-20 strain DV-104-21 strain 3200 2300 1500 Example 6
The process described in Example 2 or 3 is followed, except that instead of the Scenedesmus o,,tisiusc:ul-us strain, a Chlorella minitissima alga strain is used which is capatble to readily incorporate selenium. The selenium content of the thus-obtained alga powder is 1400 /ug/g.
Ex2nnL-le 7 The process described in Example 2 or 3 is followed, except that the nutritive solution is let to warm to a temperature of at most 50 0 C and an Aphanocapsa thermalis alga strain is used which is thermoduric and capable to readily incorporate selenium. The selenium content of the thus-obtained alga powder is as follows:
z j I v v __------ Strain Selenium content (/ug/g of alga powder) "WilC strain DV-12-220 strain OV-12-340 strain 1100 1550 Example 8
The Process described in Example 2 is followed, except that instead of the Scenedesmus obtisiusculus strain, a Spirulina sp. alga strain belonging to the blue algae is used which is capable to readily incorporate selenium. The selenium content of the thus-obtained alga powder is as follows:
Strain Selenium content (/ug/g of alga powder) ------------------------------------------- "Wild" I strain "WilC II strain HE-87-104 strain HE-89-241 strain HE-89-302 strain 1100 1200 1500 k_ Example 9
The process described in Example 2 is followed,. except that the temperature of the nutritive solution is descreased to 8 0 C and a filiform blue Nostoc commune alga strain is used which is cold-resistant and capable to readily incorporate selenium. The selenium content of the thus-obtained alga powder is as follows:.
Strain Selenium content (/ug/g of alga powder) "WilC strain BK-1218-2 strain 1140 Example 10 5 x 10- 3 M of sodium seli-enite are added to 8 litres of Bold's culture medium sterilized by filtration and filled in an alga- cultivating bottle of 10 litres volume. The thus-obtained culture medium is inoculated with a pure culture of Chlorella fusca prepared as described in Example 1 and capable to readily incorporate selenium. The alga is cultivated for 14 days as described in Example 2. Then, the alga mass is filtered, carefully washed with water and dried below 80 OC. The selenium content of the thus-obtained alga p'owder is 2700 /ug/g.
iz i Example 11
A Scenedesmus abliquus strain cultivated in the way as described in Example 1 and capable to readily incorporate selenium is inoculated into a cultivating bottle containing -5 8 litres of Bold's nutritive solution and 10- 7 M of sodium selenite. Thereafter, the process of Example 10 is followed to obtain an alga powder with a selenium content of 300 /ug/g.
Example 12
The process of Example 2 is followed, except that separated alga is dried at 65 OC and then decomposed by grinding to a particle size of 1 /um. The selenium content of the thus-obtained alga powder is 1200 /ug/g.
1 k a
Claims (1)
- C 1 a i m S1. A process for the preparation of algae having an improved biological effect, wherein the selected alga strain is cultivated under sterile conditions on a culture medium consisting of fresh water and nutritive salts in the presence of carbon dioxide and light in a photosynthetic way, or with the exclusion of light on a culture medium containing also a source of carbon, hydrogen, oxygen and nitrogen, whereafter the thus-obtained alga is separated, which c o m p r i s e s adding inorganic and/or organic selenium compound(s) to the culture medium in a concentration between 10- 7 and 2x10- 3 mole/litre, inoculating the culture medium with a pure alga culture capable to readily incorporate selenium, if desired, drying the alga obtained after cultivation and decomposing it by supersound or grinding, wherein the pure alga culture is obtained by inoculating the selected alga strain onto a liquid culture medium containing inorganic and optionally organic nutritive materials, treating it with N-methyl-NI -nitro-N-nitrosoguanidine, then cultivating the strain on a culture medium also containing selenium and finally selecting the individuals readily incorporating selenium and having a growth rate which is at least indentical to that of the original strain.2. A process as claimed in claim 1, which comprises using sodium selenite and/or selenium dioxide as inorganic selenium compound(s) in the culture medium.3. A process as claimed in claim 1, which 4 1 c o m p r i s e s using sele-nocysteine and/or selencrT,.ethior.ine as organic selenium compound(s) in the culture medium.4. A process as claimed in claim 1, which c o m p r 1 s e s using a Chlorella or Scenedesmus alga strain as selected alga strain.5. A process as claimed in claim 1, which c o m p r i s e s using Aphanocapsa thermalis as selected alga strain.6. A process as claimed in claim 1, which c o m p r i s e s using Nostoc commune as selected alga strain.. A process as claimed in claim 1, whizh S e S using Chlorella minitiss.irr,a or r-'h; ^^a c, re se.Lected alga strain.A process as claimed in claim 1, which c o m p r i s e s using a a fresh-water blue alga strain as selected alga strain.9. A process as claimed in claim 8, which c 0 m p r i S e S using a Spirulina sp. as blue alga strain.10. A process as claimed in claim 1, which c o m p r i s e s obliquus as selected alga strain.c o m p r i fusca as 8.using Scenedesmus obtic-siuscilus or 11. A process for the preparation of algae as claimed in claim 1 substantially as hereinbefore described in any one of Examples 1 to 12.12. Algae, optionally in dried and/or decomposed form, produced by a process as claimed in any one of claims 1 to 11.Shed 198 -- 9 at The Patent Office, State House, 6671 High HoIboM,LondonWC1R4TP- FurLher copies maybe obtained from The Patent office.Sales Branch, St Mary Cray, Orpington, Kent BR5 3RD. Printed by Multiplex techniques ltd, St Mary Cray, Kent, Cor, 1/87
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| HU88575A HU202272B (en) | 1988-02-09 | 1988-02-09 | Process for producing algae |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| GB8806532D0 GB8806532D0 (en) | 1988-04-20 |
| GB2214928A true GB2214928A (en) | 1989-09-13 |
| GB2214928B GB2214928B (en) | 1992-01-15 |
Family
ID=10950225
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB8806532A Expired - Fee Related GB2214928B (en) | 1988-02-09 | 1988-03-18 | Process for preparing algae incorporating selenium |
Country Status (26)
| Country | Link |
|---|---|
| JP (1) | JPH01215278A (en) |
| KR (1) | KR890014024A (en) |
| CN (1) | CN1034842A (en) |
| AT (1) | AT389888B (en) |
| AU (1) | AU606138B2 (en) |
| BE (1) | BE1001675A3 (en) |
| CA (1) | CA1310927C (en) |
| CH (1) | CH676605A5 (en) |
| DD (1) | DD267994A5 (en) |
| DK (1) | DK148888A (en) |
| ES (1) | ES2006114A6 (en) |
| FI (1) | FI881310A (en) |
| FR (1) | FR2626890B1 (en) |
| GB (1) | GB2214928B (en) |
| GR (1) | GR1000446B (en) |
| HU (1) | HU202272B (en) |
| IL (1) | IL85777A (en) |
| IT (1) | IT1216140B (en) |
| LU (1) | LU87172A1 (en) |
| NL (1) | NL8800692A (en) |
| NO (1) | NO881215L (en) |
| PL (1) | PL271284A1 (en) |
| PT (1) | PT87016B (en) |
| SE (1) | SE468815B (en) |
| YU (1) | YU55488A (en) |
| ZA (1) | ZA881956B (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CZ300809B6 (en) * | 2007-11-02 | 2009-08-12 | Mikrobiologický ústav AV CR, v.v.i. | Industrial strain Scenedesmus quadricauda SeVI of green chlorococcale alga Scenedesmus quadricauda (Turpin) Brebisson |
| CZ300808B6 (en) * | 2007-11-02 | 2009-08-12 | Mikrobiologický ústav AV CR, v.v.i. | Industrial strain Scenedesmus quadricauda SeIV+VI of green chlorococcale alga Scenedesmus quadricauda (Turpin) Brebisson |
| CZ300861B6 (en) * | 2007-11-02 | 2009-08-26 | Mikrobiologický ústav AV CR, v.v.i. | Industrial strain SeIV of green chlorococcale alga Scenedesmus quadricauda (Turpin) Brebisson |
| WO2010023384A1 (en) * | 2008-08-29 | 2010-03-04 | Eco Solution | Photosynthetic microorganisms enriched in selenium using selenohydroxy acid compounds, uses thereof in nutrition, cosmetics and pharmacy |
| KR101413841B1 (en) * | 2009-02-04 | 2014-07-02 | 클로렐라고교 가부시끼가이샤 | Selenium-containing unicellular microalgae for animal plankton feeds and method of culturing selenium-containing animal planktons using the same |
| TWI495725B (en) * | 2009-08-28 | 2015-08-11 | Metabolium | Photosynthetic microorganisms enriched in selenium using selenohydroxy acid compounds, uses thereof in nutrition, cosmetics and pharmacy |
| MD4395C1 (en) * | 2014-11-28 | 2016-08-31 | Институт Зоологии Академии Наук Молдовы | Strain of green microalga Scenedesmus quadricauda var. quadricauda - source of proteins, glucides and lipids |
| RU2774314C1 (en) * | 2021-03-14 | 2022-06-17 | Мария Максимовна Турьева | METHOD FOR CULTIVATING MICROALGAE CHLORELLA VULGARIS BEIJER. f. GLOBOSA V. ANDR IPPAS C-2024 IN NATURE USING POND WATER |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5211003A (en) * | 1992-02-05 | 1993-05-18 | General Electric Company | Diffuser clean air bleed assembly |
| EP1731597A1 (en) * | 2005-06-06 | 2006-12-13 | Johannes Gutenberg-Universität Mainz | Selenium-enriched liquid media for the cultivation of siliceous sponges and for biogenic silica production |
| WO2018199723A2 (en) * | 2017-04-28 | 2018-11-01 | 한남바이오 주식회사 | Selenium resistive novel microalgae |
| CN110317769B (en) * | 2019-07-18 | 2021-04-20 | 恩施硒圣植物科技有限公司 | Culture method of selenium-rich microalgae |
| JP2023534775A (en) * | 2020-07-08 | 2023-08-14 | カウンシル・オブ・サイエンティフィック・アンド・インダストリアル・リサーチ・アン・インディアン・レジスタード・ボディ・インコーポレイテッド・アンダー・ザ・レジストレーション・オブ・ソサエティーズ・アクト・(アクト・21・オブ・1860) | Edible marine microalgal biomass enriched with organic selenium |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DD246789A (en) * | ||||
| FR2426404A1 (en) * | 1978-05-26 | 1979-12-21 | Brevier Jean | Culture of phytoplankton in sea water - enriched with minerals, vitamin(s) and aminoacid(s), used in cosmetics and in human and veterinary medicine |
| DE3421644A1 (en) * | 1984-06-09 | 1985-12-12 | Richard 7880 Bad Säckingen Hau | DIET DIAGRAM |
| JPH062047B2 (en) * | 1985-10-11 | 1994-01-12 | 有限会社サン・ファーム | Spirulina containing high concentration of trace elements in human body and production method thereof |
| ES2000191T3 (en) * | 1986-03-19 | 1993-07-16 | Biotechna Limited | IMPROVEMENTS IN THE PRODUCTION OF BIOMASS. |
-
1988
- 1988-02-09 HU HU88575A patent/HU202272B/en not_active IP Right Cessation
- 1988-03-17 CH CH1010/88A patent/CH676605A5/de not_active IP Right Cessation
- 1988-03-18 AU AU13262/88A patent/AU606138B2/en not_active Ceased
- 1988-03-18 YU YU00554/88A patent/YU55488A/en unknown
- 1988-03-18 NL NL8800692A patent/NL8800692A/en not_active Application Discontinuation
- 1988-03-18 CN CN88101399A patent/CN1034842A/en active Pending
- 1988-03-18 IL IL85777A patent/IL85777A/en unknown
- 1988-03-18 PT PT87016A patent/PT87016B/en not_active IP Right Cessation
- 1988-03-18 PL PL27128488A patent/PL271284A1/en unknown
- 1988-03-18 IT IT8819836A patent/IT1216140B/en active
- 1988-03-18 BE BE8800310A patent/BE1001675A3/en not_active IP Right Cessation
- 1988-03-18 JP JP63063751A patent/JPH01215278A/en active Pending
- 1988-03-18 CA CA000561888A patent/CA1310927C/en not_active Expired - Fee Related
- 1988-03-18 SE SE8800991A patent/SE468815B/en not_active IP Right Cessation
- 1988-03-18 DK DK148888A patent/DK148888A/en not_active Application Discontinuation
- 1988-03-18 KR KR1019880002909A patent/KR890014024A/en not_active Application Discontinuation
- 1988-03-18 ZA ZA881956A patent/ZA881956B/en unknown
- 1988-03-18 ES ES8800828A patent/ES2006114A6/en not_active Expired
- 1988-03-18 AT AT0073588A patent/AT389888B/en not_active IP Right Cessation
- 1988-03-18 FR FR888803516A patent/FR2626890B1/en not_active Expired - Fee Related
- 1988-03-18 DD DD31381788A patent/DD267994A5/en unknown
- 1988-03-18 GB GB8806532A patent/GB2214928B/en not_active Expired - Fee Related
- 1988-03-18 FI FI881310A patent/FI881310A/en not_active Application Discontinuation
- 1988-03-18 GR GR880100171A patent/GR1000446B/en unknown
- 1988-03-18 NO NO88881215A patent/NO881215L/en unknown
- 1988-03-18 LU LU87172A patent/LU87172A1/en unknown
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| CZ300808B6 (en) * | 2007-11-02 | 2009-08-12 | Mikrobiologický ústav AV CR, v.v.i. | Industrial strain Scenedesmus quadricauda SeIV+VI of green chlorococcale alga Scenedesmus quadricauda (Turpin) Brebisson |
| CZ300861B6 (en) * | 2007-11-02 | 2009-08-26 | Mikrobiologický ústav AV CR, v.v.i. | Industrial strain SeIV of green chlorococcale alga Scenedesmus quadricauda (Turpin) Brebisson |
| CN102137922A (en) * | 2008-08-29 | 2011-07-27 | Eco解决方案公司 | Photosynthetic microorganisms enriched in selenium using selenohydroxy acid compounds, uses thereof in nutrition, cosmetics and pharmacy |
| FR2935394A1 (en) * | 2008-08-29 | 2010-03-05 | Eco Solution | ORGANIC SELENIUM-ENRICHED PHOTOSYNTHETIC MICROORGANISMS FROM SELENO-HYDROXYACID COMPOUNDS AND THEIR USE IN NUTRITION, COSMETICS AND PHARMACY |
| US20110165658A1 (en) * | 2008-08-29 | 2011-07-07 | Eco Solution | Photosynthetic microorganisms enriched in selenium using selenohydroxy acid compounds, used thereof in nutrition, cosmetics and pharmacy |
| WO2010023384A1 (en) * | 2008-08-29 | 2010-03-04 | Eco Solution | Photosynthetic microorganisms enriched in selenium using selenohydroxy acid compounds, uses thereof in nutrition, cosmetics and pharmacy |
| JP2012500647A (en) * | 2008-08-29 | 2012-01-12 | エコ ソルーシヨン | Photosynthetic microorganisms enriched with selenium using selenohydroxy acid compounds and their use in nutritional foods, cosmetics and medicines |
| CN102137922B (en) * | 2008-08-29 | 2013-10-30 | Eco解决方案公司 | Photosynthetic microorganisms enriched in selenium using selenohydroxy acid compounds, uses thereof in nutrition, cosmetics and pharmacy |
| RU2504578C2 (en) * | 2008-08-29 | 2014-01-20 | Эко Солюсьон | Photosynthesised microorganisms rich in selenium from selenium-containing hydroxy acids, use thereof in food, cosmetics and pharmacy |
| US9017985B2 (en) * | 2008-08-29 | 2015-04-28 | Metabolium | Photosynthetic microorganisms enriched in selenium using selenohydroxy acid compounds, used thereof in nutrition, cosmetics and pharmacy |
| KR101413841B1 (en) * | 2009-02-04 | 2014-07-02 | 클로렐라고교 가부시끼가이샤 | Selenium-containing unicellular microalgae for animal plankton feeds and method of culturing selenium-containing animal planktons using the same |
| TWI495725B (en) * | 2009-08-28 | 2015-08-11 | Metabolium | Photosynthetic microorganisms enriched in selenium using selenohydroxy acid compounds, uses thereof in nutrition, cosmetics and pharmacy |
| MD4395C1 (en) * | 2014-11-28 | 2016-08-31 | Институт Зоологии Академии Наук Молдовы | Strain of green microalga Scenedesmus quadricauda var. quadricauda - source of proteins, glucides and lipids |
| RU2774314C1 (en) * | 2021-03-14 | 2022-06-17 | Мария Максимовна Турьева | METHOD FOR CULTIVATING MICROALGAE CHLORELLA VULGARIS BEIJER. f. GLOBOSA V. ANDR IPPAS C-2024 IN NATURE USING POND WATER |
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| Date | Code | Title | Description |
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| PCNP | Patent ceased through non-payment of renewal fee |
Effective date: 19940318 |