Banana stem leaves is utilized to make the method for stocker biological feedstuff
Technical field
The present invention discloses a kind of biological feedstuff production method, specifically utilizes crop by-products to produce the production method of stocker feed.
Background technology
Along with the raising of people's living standard, the demand of people to meat food is increasing, particularly the demand of high-grade meat, thus has effectively promoted the development of animal husbandry.The development of livestock breeding industry, needing a large amount of grain feeds for supporting, how to avoid livestock breeding industry and people to strive grain, opening up new feed resource, becoming the problem that people are concerned about.
A large amount of discarded objects is obtained after annual agricultural production results cereal, as the string such as stalk, shell class material, these materials are reproducible resources, and these materials are by after hydrolysis, a kind of material can be obtained---glucose sugar, and glucose is the energy matter of animal.If these string class materials are developed to feed, not only can save traditional feed resource, and can improve the ecological environment, form the benign cycle of agricultural production, promote the sustainable development of agricultural, animal husbandry.
Guangxi is the second main producing region of whole nation plantation banana, banana industry nearly 2 years growth momentums in Guangxi are swift and violent, cultivated area increases to 146.5 ten thousand mu in 2013 from 2009 1,100,000 mu, according to every per mu yield 2 ~ 3 tons calculating, the banana annual production in Guangxi more than 3,000,000 tons, the simultaneously banana stem leaves of the up to ten million ton of by-product.Crude protein content 6 ~ 12% in banana stem leaves dry, crude fibre 20 ~ 30%, crude fat 1 ~ 5%, ash content 12 ~ 25%, ruminant can digest banana stem leaves preferably, is 65% to the digestibility of leaf, be 75% to the digestibility of stem, thus banana stem leaves is a kind of feed with potentiality to be exploited.But fresh banana stem leaves water content is greater than 95%, must could preserve for a long time by drying and dewatering, and traditional dewatering energy consumption is high, labour intensity is large, and banana stem leaves contains a large amount of tannin, can cause intestinal obturation after animal searches for food in a large number, and ight soil is hard.
Summary of the invention
The object of the invention is open a kind of biological feedstuff production method, it is the production method utilizing banana stem leaves and urea production stocker feed, the tannin in banana stem leaves can be destroyed, improve the palatability of raw material, improve digestibility, and can long term storage and corruption of not going mouldy.
Technical scheme of the present invention is:
Banana stem leaves and urea make the method for stocker biological feedstuff, comprise the steps:
(1) banana stem leaves pulverizer is pulverized, and crosses screen cloth, gets the particle between 2 ~ 10 orders;
(2) particle obtained above adds the urea of its weight 0.5 ~ 1.5%, mixes, and then uses 120 ~ 125 DEG C of steam treatment 30 ~ 60min, is cooled to less than 30 DEG C, add Trichoderma viride liquid medium, cultivates 24 ~ 48h;
(3) material that step (2) obtains adds the mould liquid medium of healthy and free from worry wood, cultivates 24 ~ 48h;
(4) material that step (3) obtains adds lactic acid bacteria liquid medium, seals at least 7 days, obtains stocker biological feedstuff.
The production method of described Trichoderma viride liquid medium: every 1 liter of potato extract adds glucose 20g, potassium dihydrogen phosphate 2g and ammonium sulfate 5g, access Trichoderma viride bacterial classification (Classification system Trichodermaviride) after conventional sterilant, 16h cultivated by shaking table; Described potato extract: get peeled potatoes 200g, be cut into small pieces, the 1.0L that adds water boils 30min, and elimination potato ball, complements to 1.0L by filtrate.Trichoderma viride belongs to Deuteromycotina, Hyphomycetes, hyphomycetales, and sticky spore mushroom is the ubiquitous fungi of a class, and Trichoderma viride is one of institute's cellulase-producing the highest active bacterial strain, and the cellulase produced has degradation to crop, and effect is very good.
The production method of the described mould liquid medium of healthy and free from worry wood: every 1 liter of potato extract adds glucose 20g, potassium dihydrogen phosphate 2g and ammonium sulfate 5g, access healthy and free from worry Trichoderma kind (Classification system TrichodermaKoningii) after conventional sterilant, 16h cultivated by shaking table; Described potato extract: get peeled potatoes 200g, be cut into small pieces, the 1.0L that adds water boils 30min, and elimination potato ball, complements to 1.0L by filtrate.Healthy and free from worry Trichoderma has very strong cellulose mycophenolate and fiber, two saccharogenic amylases, lignoenzyme etc., and it can be beneficial to agricultural byproducts, and as straw, timber, the cellulosic materials such as wood chip, make it to change saccharine material into.
The production method of described lactic acid bacteria liquid medium: every 1 liter of distilled water adds peptone 5.0g, beef extract 10g, yeast extract 5.0g, glucose 10.0g and NaCl5.0g, access lactic acid bacteria culturers (Classification system LactococcusLactis) after conventional sterilant, 16h cultivated by shaking table.Lactic acid bacteria can produce a large amount of lactic acid from fermentability carbohydrate, and the lactic acid of generation can suppress the growth of other miscellaneous bacterias, thus the shelf-life of food and extend.
The good effect that the present invention produces is: the effect utilizing multiple-microorganism, particularly Trichoderma viride and healthy and free from worry wood mould, the tannin can degraded in banana stem leaves, and the cellulose of degrading in banana stem leaves and lignin, make the feed made soft, improve palatability, improve digestibility, and by the fermentation of lactic acid bacteria, increase the fragrance of feed, and can long term storage feed, prevent feed mold.
Detailed description of the invention
Embodiment 1
Banana stem leaves pulverizer is pulverized, and crosses screen cloth, gets the particle between 2 ~ 10 orders; Add the urea of particle weight 0.5%, mix rear use 120 ~ 125 DEG C of steam treatment 30 ~ 60min, be cooled to less than 30 DEG C, add the Trichoderma viride liquid medium of banana stem leaves weight 1%, cultivate 24h; Add the mould liquid medium of healthy and free from worry wood of banana stem leaves weight 1% again, cultivate 24 ~ 48h; Finally add the lactic acid bacteria liquid medium of banana stem leaves weight 1%, seal at least 7 days, obtain stocker biological feedstuff;
The production method of above-mentioned Trichoderma viride liquid medium: every 1 liter of potato extract adds glucose 20g, potassium dihydrogen phosphate 2g and ammonium sulfate 5g, accesses Trichoderma viride bacterial classification after conventional sterilant, 16h cultivated by shaking table; The production method of the above-mentioned mould liquid medium of healthy and free from worry wood: every 1 liter of potato extract adds glucose 20g, potassium dihydrogen phosphate 2g and ammonium sulfate 5g, accesses healthy and free from worry Trichoderma kind after conventional sterilant, 16h cultivated by shaking table; Above-mentioned potato extract: get peeled potatoes 200g, be cut into small pieces, the 1.0L that adds water boils 30min, and elimination potato ball, complements to 1.0L by filtrate.The production method of above-mentioned lactic acid bacteria liquid medium: every 1 liter of distilled water adds peptone 5.0g, beef extract 10g, yeast extract 5.0g, and glucose 10.0g and NaCl5.0g, accesses lactic acid bacteria culturers after conventional sterilant, 16h cultivated by shaking table.
Embodiment 2
Banana stem leaves pulverizer is pulverized, and crosses screen cloth, gets the particle between 2 ~ 10 orders, add the urea of particle weight 1%, mix rear use 120 ~ 125 DEG C of steam treatment 45min, be cooled to less than 30 DEG C, add the Trichoderma viride liquid medium of banana stem leaves weight 2%, cultivate 24h; Add the mould liquid medium of healthy and free from worry wood of banana stem leaves weight 2% again, cultivate 24 ~ 48h; Finally add the lactic acid bacteria liquid medium of banana stem leaves weight 2%, seal at least 7 days, obtain stocker biological feedstuff;
The production method of Trichoderma viride liquid medium, the mould liquid medium of healthy and free from worry wood and lactic acid bacteria liquid medium is with embodiment 1.
Embodiment 3
Banana stem leaves pulverizer is pulverized, and crosses screen cloth, gets the particle between 2 ~ 10 orders, add the urea of particle weight 1.5%, mix rear use 120 ~ 125 DEG C of steam treatment 60min, be cooled to less than 30 DEG C, add the Trichoderma viride liquid medium of banana stem leaves weight 2%, cultivate 24h; Add the mould liquid medium of healthy and free from worry wood of banana stem leaves weight 2% again, cultivate 24 ~ 48h; Finally add the lactic acid bacteria liquid medium of banana stem leaves weight 2%, seal at least 7 days, obtain stocker biological feedstuff;
The production method of Trichoderma viride liquid medium, the mould liquid medium of healthy and free from worry wood and lactic acid bacteria liquid medium is with embodiment 1.
The quality index of the stocker biological feedstuff of gained of the present invention is as follows:
Get biological feedstuff of the present invention, banana stem leaves, hybrid giant napier, bagasse and put into beef cattle cowshed simultaneously, allow ox freely take food, result beef cattle only selects biological feedstuff of the present invention, and selects banana stem leaves, hybrid giant napier or bagasse without a head of cattle.
Get following diet 20 oxen, sooner or later give twice feed every day, the daily gain situation of ox is as shown in the table:
Beef cattle biological feedstuff color and luster of the present invention is yellowish, smell delicate fragrance, and quality is soft, easily by ruminant digestion, be used for separately cattle feeding, daily gain reaches more than 350g, with energy feed and fine fodder together cattle feeding, most fine fodder can be saved, reduce the feed cost of steer ration.