CN113913324A - Composite microbial inoculum for efficient deodorization of biological filter and preparation method thereof - Google Patents
Composite microbial inoculum for efficient deodorization of biological filter and preparation method thereof Download PDFInfo
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- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C02F2303/00—Specific treatment goals
- C02F2303/02—Odour removal or prevention of malodour
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Abstract
The invention discloses a composite microbial inoculum for efficiently deodorizing a biological filter, which comprises the following components: 50-70 parts of alcaligenes faecalis, 50-70 parts of saccharomyces cerevisiae, 30-60 parts of lactobacillus delbrueckii, 30-60 parts of bacillus subtilis, 15-25 parts of bacillus megaterium, 15-25 parts of thiobacillus denitrificans, 10-20 parts of thiobacillus oxydans and 10-20 parts of EM bacteria; the preparation method comprises the following steps: (1) weighing the raw materials; (2) activating strains; (3) compounding mixed strains; (4) preparing a first-level seed solution; (5) and (3) preparing the compound microbial inoculum. The invention utilizes the microbial functional flora to absorb, decompose and convert odor molecules into odorless substances, inhibits the growth of odor-producing microorganisms through the antagonistic action of the microorganisms, reduces the generation and release of malodorous gases such as ammonia, hydrogen sulfide and the like in the biofiltration process, and thus achieves the purpose of eliminating or inhibiting the generation of odor; and the preparation is simple, the deodorization is rapid, the effect is durable, and no secondary pollution is caused.
Description
Technical Field
The invention relates to the technical field of composite bacteria, in particular to a composite bacteria for efficiently deodorizing a biological filter and a preparation method thereof.
Background
The biological filter is used for tail end deodorization in urban sewage treatment facilities and organic aerobic fermentation engineering. When the odor passes through the filter, the odor is in clearance contact with a microbial film growing on the surface of the filler, so that foreign (odor) odor, volatile substances (VOC) and harmful substances in the odor can be removed, and the waste gas is purified, thereby achieving the purposes of deodorization and standard emission. The specific application range comprises: removing odor in urban sewage treatment facilities, production odor in the garbage treatment process, production odor in sludge composting plants, odor generated in coating and spray painting, coking, excrement treatment process and the like. The biological filtration method can degrade most volatile and semi-volatile alkanes, alkenes and aromatics, and the substances generally have the characteristics of biodegradability and high water solubility. Substances that have been tested for removal by biofiltration include: ammonia, carbon monoxide, hydrogen sulfide, methane, methanol, ethanol, isopropanol, n-butanol, ethylhexanol, propane, isopentane, hexane, butyraldehyde, acetone, methyl ethyl ketone, butyl acetate, diethylamine, triethylamine, dimethyl disulfide, skatole, indole, methyl mercaptan, methyl chloride, ethylene, trichloroethylene, tetrachloroethylene, nitrogen oxides, dimethyl sulfide, thiophene, benzene, toluene, xylene, ethylbenzene, styrene, and the like.
The method improves the established aerobic fermentation treatment facilities of the waste water and the organic waste and builds new facilities, and the public is most concerned about that the odor can be leaked out and harms the health of the surrounding people. How to efficiently deodorize has become one of the main considerations in the design and operation of sewage treatment and organic waste treatment facilities, particularly with respect to the public's acceptance of these facilities.
Therefore, how to improve the deodorization efficiency of the biological filter is a problem to be solved urgently by the technical personnel in the field.
Disclosure of Invention
In view of the above, the present invention aims to provide a complex microbial inoculum for efficient deodorization of a biological filter and a preparation method thereof, so as to solve the defects in the prior art.
In order to achieve the purpose, the invention adopts the following technical scheme:
a composite microbial inoculum for efficiently deodorizing a biological filter comprises the following raw materials in parts by weight: 50-70 parts of alcaligenes faecalis, 50-70 parts of saccharomyces cerevisiae, 30-60 parts of lactobacillus delbrueckii, 30-60 parts of bacillus subtilis, 15-25 parts of bacillus megaterium, 15-25 parts of thiobacillus denitrificans, 10-20 parts of thiobacillus oxydans and 10-20 parts of EM bacteria;
preferably: 55-65 parts of alcaligenes faecalis, 55-65 parts of saccharomyces cerevisiae, 40-50 parts of lactobacillus delbrueckii, 40-50 parts of bacillus subtilis, 18-22 parts of bacillus megaterium, 18-22 parts of thiobacillus denitrificans, 12-18 parts of thiobacillus oxydans and 12-18 parts of EM (effective microorganisms);
more preferably: 60 parts of alcaligenes faecalis, 60 parts of saccharomyces cerevisiae, 45 parts of lactobacillus delbrueckii, 45 parts of bacillus subtilis, 20 parts of bacillus megaterium, 20 parts of thiobacillus denitrificans, 15 parts of thiobacillus oxydans and 15 parts of EM (effective microorganisms).
Further, the Alcaligenes faecalis is strain M50-B1, which is deposited in China general microbiological culture Collection center (CGMCC for short, address: No.3 of West Lu 1 of Beijing Kogyo area, Microbiol research institute of Chinese academy of sciences, zip code 100101) at 9/5.2011, and is classified and named as Alcaligenes faecalis, with the preservation number: CGMCC No. 5219.
Furthermore, the Saccharomyces cerevisiae is (Saccharomyces cerevisiae) ZGFJ-11, the strain is preserved in China general microbiological culture Collection center of China Committee for culture Collection of microorganisms, and the preservation address is as follows: the product is prepared from the Hongyang district Tunglu of Beijing, institute of microbiology of Chinese academy of sciences, zip code 100101, preservation date of 2009, 7 months and 30 days, and preservation numbers: CGMCC No. 3217.
Further, the Lactobacillus delbrueckii subsp. bulgaricus inm25-LB, and the strain has a preservation date of: 12/03/2018, the preservation unit is: the China general microbiological culture Collection center has the following preservation addresses: xilu No.1 Hospital No.3, Beijing, Chaoyang, with the deposit number: CGMCC No. 15445.
Further, the Bacillus subtilis Bs-03 is obtained by separating from experimental nursery soil of forestry science research institute in Shandong province by a soil dilution separation method, and has been deposited in the general microbiological culture center of China Committee for microbiological Collection on 23.04.2009, address: the collection number of the microbial research institute of the Chinese academy of sciences, No.3 Xilu-Beijing province, Chaoyang, and the collection number is: CGMCC No. 3038.
Further, the Bacillus megaterium (BM 1259) is a strain isolated and extracted from soil and having unique effects of deodorization, and has been deposited in the chinese collection of microorganisms with the following deposit numbers: GMCC No. 1259.
Further, the above-mentioned thiobacillus denitrificans is Geobacillus thermoglucopyranosidus, which is deposited in China General Microbiological culture Collection center (China General Microbiological culture Collection center) at 12.12.4.2015, and the deposition address is as follows: west road No.1, north west of the township, beijing, ministry of sciences, china, institute of microbiology, zip code: 100101, accession number: CGMCC NO. 11780.
Further, the thiobacillus oxydans is Acidithiobacillus thiooxidans ZJ-1, is preserved in China general microbiological culture Collection center (CGMCC), has the preservation date of 2016, 4 and 21 days, has the preservation address of China academy of sciences microorganism institute No.3, North West Lu No.1 Hospital, Indormitong, Beijing, and has the preservation number of CGMCC No. 12387.
Further, the EM is a microbial preparation compounded by 10 microorganisms of more than 80 genera mainly including photosynthetic bacteria, lactic acid bacteria, yeast and actinomycetes.
Further, the total effective viable count of the microbial inoculum is more than or equal to 1 multiplied by 1010CFU/g。
A preparation method of a composite microbial inoculum for efficiently deodorizing a biological filter comprises the following steps:
(1) weighing the raw materials
Weighing each strain according to the weight part of the composite microbial inoculum;
(2) strain activation
Respectively inoculating each strain to solid culture medium, culturing at 50-55 deg.C for 16-20h, selecting single colony to be inoculated to slant culture medium, culturing at 50-55 deg.C for 16-20h, washing with sterile water to obtain surface thallus as inoculation liquid with viable bacteria amount greater than 1 × 1010CFU/g；
(3) Compounding of mixed strains
Mixing the inoculation solutions of the strains to obtain a compound inoculation solution;
(4) preparation of first-order seed liquid
Inoculating the complex seed solution into the solid culture medium according to the inoculum size of 5-10% of the volume ratio, and culturing for 16-20h at the rotating speed of a shaking table of 180-220rpm and the temperature of 50-55 ℃ to obtain a first-stage seed solution;
(5) preparing a complex microbial inoculum:
inoculating the first-stage seed liquid into the fermentation culture medium according to the inoculation amount of 0.5-2% of the volume ratio, wherein the ventilation amount is 6-8m3The stirring speed is 180-10And when the concentration is CFU/g, the compound microbial inoculum is obtained.
Further, in the step (2), the solid medium and the slant medium both have the following compositions: 10g of peptone, 5g of beef extract, 5g of sodium chloride, 2g of yeast powder and 20g of agar, adding water to a constant volume of 1L, and adjusting the pH value to 7.2; in the step (5), the fermentation medium comprises the following components: 15g/L of brown sugar, 1.0g/L of ammonium sulfate, 3g/L of peptone, 2g/L of yeast powder and 5g/L of sodium chloride, adding water to a constant volume of 1L, and adjusting the pH value to 7.2.
The invention also discloses a use method of the composite microbial inoculum for efficiently deodorizing the biological filter, which comprises the following steps: the composite microbial inoculum with the deodorization air volume of one ten thousandth to three ten thousandth of the deodorization air volume per hour is added into the biological filter once per month.
According to the technical scheme, compared with the prior art, the invention has the following beneficial effects:
1. the alcaligenes faecalis provided by the invention can oxidize main odor components by hydrogen sulfide to form sulfur polymers, does not generate sulfuric acid, and does not have secondary pollution; and the alcaligenes faecalis can be proliferated rapidly in the environment, and occupies a certain ecological position while removing hydrogen sulfide, thereby reducing the generation of odor and fundamentally solving the problem of odor.
2. The saccharomyces cerevisiae, the lactobacillus delbrueckii, the bacillus subtilis, the thiobacillus oxydans and the bacillus megaterium provided by the invention can quickly and effectively adsorb and eliminate malodorous gas components.
3. The nitrogen source range that the thiobacillus denitrificans can utilize is very wide, can be ammonia salt, nitrate, nitrite, amino acid and the like, and under the anaerobic condition, the thiobacillus denitrificans participates in sulfur and nitrogen circulation in a denitrification reaction mode simultaneously, thereby effectively absorbing and eliminating odor substances such as methyl mercaptan, ammonia gas and the like.
4. The EM bacteria provided by the invention can control the content of substances such as ammonia, hydrogen sulfide and the like in the water environment of the filter tank, purify the water quality and improve the environment; pathogenic bacteria are reduced, and the microecology is balanced; promoting the decomposition of organic pollutants, reducing BOD and COD and improving the odor purification efficiency.
6. The composite microbial inoculum provided by the invention utilizes the microbial functional flora to absorb, decompose and convert odor molecules into odorless substances, inhibits the growth of odor-producing microorganisms through the antagonistic action of the microorganisms, and reduces the generation and release of new malodorous gases such as ammonia, hydrogen sulfide and the like in the biofiltration process, thereby achieving the purpose of eliminating or inhibiting the generation of odor; and the preparation is simple, the deodorization is rapid, the effect is durable, and no secondary pollution is caused.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In examples 1-5 below:
1. the Alcaligenes faecalis is an Alcaligenes faecalis strain M50-B1, which is preserved in China general microbiological culture Collection center (CGMCC for short, address: No.3 of West Lu 1 of the Beijing Kogyo of the morning area, China academy of sciences microbiological research institute, postal code 100101) at 9/5.2011, and is classified and named as Alcaligenes faecalis (Alcaligenes faecalis), wherein the preservation number is as follows: CGMCC No. 5219.
2. The beer yeast is (Saccharomyces cerevisiae) ZGFJ-11, and the strain is preserved in China general microbiological culture Collection center of China Committee for culture Collection of microorganisms with the preservation address: the product is prepared from the Hongyang district Tunglu of Beijing, institute of microbiology of Chinese academy of sciences, zip code 100101, preservation date of 2009, 7 months and 30 days, and preservation numbers: CGMCC No. 3217.
3. The Lactobacillus delbrueckii is Lactobacillus delbrueckii subsp. bulgaricus inm25-LB, and the preservation date of the strain is as follows: 12/03/2018, the preservation unit is: the China general microbiological culture Collection center has the following preservation addresses: xilu No.1 Hospital No.3, Beijing, Chaoyang, with the deposit number: CGMCC No. 15445.
4. Bacillus subtilis Bs-03 is obtained by separating from experimental nursery soil of Shandong province forestry scientific research institute by a soil dilution separation method, and is preserved in China general microbiological culture Collection center (China Committee for culture Collection of microorganisms) at 23.04.2009, with the address: the collection number of the microbial research institute of the Chinese academy of sciences, No.3 Xilu-Beijing province, Chaoyang, and the collection number is: CGMCC No. 3038.
5. Bacillus megaterium (BM 1259) is a strain separated and extracted from soil and has unique functions of deodorization, and is preserved in China center for culture of microorganisms with the preservation number: GMCCNo. 1259.
6. Thiobacillus denitrificans is Geobacillus thermoglucopyranosidus and is preserved in China General Microbiological culture Collection center (China General Microbiological culture Collection center) in 12-month and 4-month in 2015, and the preservation address is as follows: west road No.1, north west of the township, beijing, ministry of sciences, china, institute of microbiology, zip code: 100101, accession number: CGMCC NO. 11780.
7. The thiobacillus oxydans is Acidithiobacillus thiooxidans ZJ-1, is preserved in China general microbiological culture Collection center (CGMCC), has the preservation date of 2016, 4 and 21 days, has the preservation address of China academy of sciences microorganism institute No.3, North West Lu No.1 Hospital, Chaoyang, Beijing, and has the preservation number of CGMCC No. 12387.
8. The EM is a microbial preparation compounded by 10 microorganisms which are more than 80 and mainly comprise photosynthetic bacteria, lactic acid bacteria, saccharomycetes and actinomycetes.
Example 1
The composite microbial inoculum for efficiently deodorizing the biological filter comprises the following raw materials by weight: rod for producing alkali from excrement60g of bacteria, 60g of saccharomyces cerevisiae, 45g of lactobacillus delbrueckii, 45g of bacillus subtilis, 20g of bacillus megaterium, 20g of thiobacillus denitrificans, 15g of thiobacillus oxydans and 15g of EM bacteria, and the total effective viable count is more than or equal to 1 multiplied by 1010CFU/g。
The preparation method of the composite microbial inoculum for efficiently deodorizing the biological filter comprises the following steps:
(1) weighing the raw materials
Weighing each strain according to the weight of the composite microbial inoculum;
(2) strain activation
Respectively inoculating each strain to a solid culture medium, culturing at 52 deg.C for 18h, selecting single colony to be inoculated to a slant culture medium, culturing at 52 deg.C for 18h, washing the surface thallus of the culture medium with sterile water to serve as inoculation liquid, wherein the viable bacteria amount of the inoculation liquid is more than 1 × 1010CFU/g；
Wherein, the solid culture medium and the slant culture medium both comprise the following components: 10g of peptone, 5g of beef extract, 5g of sodium chloride, 2g of yeast powder and 20g of agar, adding water to a constant volume of 1L, and adjusting the pH value to 7.2;
(3) compounding of mixed strains
Mixing the inoculation solutions of the strains to obtain a compound inoculation solution;
(4) preparation of first-order seed liquid
Inoculating the complex inoculum to a solid culture medium according to the inoculum size of 8% by volume, and culturing at 52 ℃ for 18h at the rotating speed of 200rpm of a shaking table to obtain a first-stage seed solution;
(5) preparing a complex microbial inoculum:
inoculating the first-stage seed liquid into the fermentation medium according to the inoculation amount of 1% by volume, wherein the ventilation amount is 8m3H, stirring speed is 200rpm, culturing is carried out for 18h at 52 ℃, and the amount of viable bacteria in the liquid microbial inoculum is more than 1 multiplied by 1010When CFU/g is needed, the compound microbial inoculum is obtained;
wherein, the fermentation medium comprises the following components: 15g/L of brown sugar, 1.0g/L of ammonium sulfate, 3g/L of peptone, 2g/L of yeast powder and 5g/L of sodium chloride, adding water to a constant volume of 1L, and adjusting the pH value to 7.2.
Example 2
A composite bacterial agent for efficiently deodorizing a biological filter,the material comprises the following raw materials by weight: 50g of alcaligenes faecalis, 50g of saccharomyces cerevisiae, 30g of lactobacillus delbrueckii, 30g of bacillus subtilis, 15g of bacillus megaterium, 15g of thiobacillus denitrificans, 10g of thiobacillus thiooxidans and 10g of EM (effective viable count) bacteria, wherein the total effective viable count is more than or equal to 1 multiplied by 1010CFU/g。
The preparation method of the composite microbial inoculum for efficiently deodorizing the biological filter comprises the following steps:
(1) weighing the raw materials
Weighing each strain according to the weight of the composite microbial inoculum;
(2) strain activation
Respectively inoculating each strain to a solid culture medium, culturing at 50 deg.C for 16h, selecting single colony to be inoculated to a slant culture medium, culturing at 50 deg.C for 16h, washing with sterile water to obtain surface thallus on the culture medium as inoculation liquid with viable count of more than 1 × 1010CFU/g；
Wherein, the solid culture medium and the slant culture medium both comprise the following components: 10g of peptone, 5g of beef extract, 5g of sodium chloride, 2g of yeast powder and 20g of agar, adding water to a constant volume of 1L, and adjusting the pH value to 7.2;
(3) compounding of mixed strains
Mixing the inoculation solutions of the strains to obtain a compound inoculation solution;
(4) preparation of first-order seed liquid
Inoculating the complex inoculum to a solid culture medium according to an inoculum size of 5% by volume, and culturing at a shaker rotation speed of 1800rpm and 50 ℃ for 16h to obtain a first-stage seed solution;
(5) preparing a complex microbial inoculum:
inoculating the first-stage seed solution into the fermentation medium according to the inoculation amount of 0.5% by volume, wherein the ventilation amount is 6m3The stirring speed is 180rpm, the culture is carried out for 16h at the temperature of 50 ℃, and the amount of viable bacteria in the liquid microbial inoculum is more than 1 multiplied by 1010When CFU/g is needed, the compound microbial inoculum is obtained;
wherein, the fermentation medium comprises the following components: 15g/L of brown sugar, 1.0g/L of ammonium sulfate, 3g/L of peptone, 2g/L of yeast powder and 5g/L of sodium chloride, adding water to a constant volume of 1L, and adjusting the pH value to 7.2.
Example 3
The composite microbial inoculum for efficiently deodorizing the biological filter comprises the following raw materials by weight: 55g of alcaligenes faecalis, 55g of saccharomyces cerevisiae, 40g of lactobacillus delbrueckii, 40g of bacillus subtilis, 18g of bacillus megaterium, 18g of thiobacillus denitrificans, 12g of thiobacillus thiooxidans and 12g of EM (effective viable count) bacteria, wherein the total effective viable count is more than or equal to 1 multiplied by 1010CFU/g。
The preparation method of the composite microbial inoculum for efficiently deodorizing the biological filter comprises the following steps:
(1) weighing the raw materials
Weighing each strain according to the weight of the composite microbial inoculum;
(2) strain activation
Respectively inoculating each strain to a solid culture medium, culturing at 50 deg.C for 20 hr, selecting single colony, inoculating to a slant culture medium, culturing at 50 deg.C for 20 hr, washing with sterile water to obtain surface thallus as inoculation liquid with viable bacteria amount greater than 1 × 1010CFU/g；
Wherein, the solid culture medium and the slant culture medium both comprise the following components: 10g of peptone, 5g of beef extract, 5g of sodium chloride, 2g of yeast powder and 20g of agar, adding water to a constant volume of 1L, and adjusting the pH value to 7.2;
(3) compounding of mixed strains
Mixing the inoculation solutions of the strains to obtain a compound inoculation solution;
(4) preparation of first-order seed liquid
Inoculating the complex inoculum to a solid culture medium according to the inoculum size of 6% by volume, and culturing at 50 ℃ for 20h at the rotating speed of 180rpm of a shaking table to obtain a first-stage seed solution;
(5) preparing a complex microbial inoculum:
inoculating the first-stage seed liquid into the fermentation medium according to the inoculation amount of 1% by volume, wherein the ventilation amount is 6m3H, stirring speed is 180rpm, culturing is carried out for 20h at 50 ℃, and the amount of viable bacteria in the liquid microbial inoculum is more than 1 multiplied by 1010When CFU/g is needed, the compound microbial inoculum is obtained;
wherein, the fermentation medium comprises the following components: 15g/L of brown sugar, 1.0g/L of ammonium sulfate, 3g/L of peptone, 2g/L of yeast powder and 5g/L of sodium chloride, adding water to a constant volume of 1L, and adjusting the pH value to 7.2.
Example 4
The composite microbial inoculum for efficiently deodorizing the biological filter comprises the following raw materials by weight: 65g of alcaligenes faecalis, 65g of saccharomyces cerevisiae, 50g of lactobacillus delbrueckii, 50g of bacillus subtilis, 22g of bacillus megaterium, 22g of thiobacillus denitrificans, 18g of thiobacillus thiooxidans and 18g of EM bacteria, and the total effective viable count is more than or equal to 1 multiplied by 1010CFU/g。
The preparation method of the composite microbial inoculum for efficiently deodorizing the biological filter comprises the following steps:
(1) weighing the raw materials
Weighing each strain according to the weight of the composite microbial inoculum;
(2) strain activation
Respectively inoculating each strain to a solid culture medium, culturing at 55 deg.C for 16h, selecting single colony to be inoculated to a slant culture medium, culturing at 55 deg.C for 16h, washing with sterile water to obtain surface thallus on the culture medium as inoculation liquid with viable count of more than 1 × 1010CFU/g；
Wherein, the solid culture medium and the slant culture medium both comprise the following components: 10g of peptone, 5g of beef extract, 5g of sodium chloride, 2g of yeast powder and 20g of agar, adding water to a constant volume of 1L, and adjusting the pH value to 7.2;
(3) compounding of mixed strains
Mixing the inoculation solutions of the strains to obtain a compound inoculation solution;
(4) preparation of first-order seed liquid
Inoculating the complex inoculum to a solid culture medium according to the inoculum size of 10% by volume, and culturing at the rotating speed of 220rpm of a shaking table and the temperature of 55 ℃ for 16h to obtain a first-stage seed solution;
(5) preparing a complex microbial inoculum:
inoculating the first-stage seed liquid into the fermentation culture medium according to the inoculation amount of 2% of the volume ratio, wherein the ventilation amount is 8m3H, the stirring speed is 220rpm, the culture is carried out for 16h at the temperature of 55 ℃, and the amount of viable bacteria in the liquid microbial inoculum is more than 1 multiplied by 1010When CFU/g is needed, the compound microbial inoculum is obtained;
wherein, the fermentation medium comprises the following components: 15g/L of brown sugar, 1.0g/L of ammonium sulfate, 3g/L of peptone, 2g/L of yeast powder and 5g/L of sodium chloride, adding water to a constant volume of 1L, and adjusting the pH value to 7.2.
Example 5
The composite microbial inoculum for efficiently deodorizing the biological filter comprises the following raw materials by weight: 70g of alcaligenes faecalis, 70g of saccharomyces cerevisiae, 60g of lactobacillus delbrueckii, 60g of bacillus subtilis, 25g of bacillus megaterium, 25g of thiobacillus denitrificans, 20g of thiobacillus thiooxidans and 20g of EM (effective viable count) bacteria, wherein the total effective viable count is more than or equal to 1 multiplied by 1010CFU/g。
The preparation method of the composite microbial inoculum for efficiently deodorizing the biological filter comprises the following steps:
(1) weighing the raw materials
Weighing each strain according to the weight of the composite microbial inoculum;
(2) strain activation
Respectively inoculating each strain to a solid culture medium, culturing at 55 deg.C for 20h, selecting single colony to be inoculated to a slant culture medium, culturing at 55 deg.C for 20h, washing with sterile water to obtain surface thallus on the culture medium as inoculation liquid with viable count of more than 1 × 1010CFU/g；
Wherein, the solid culture medium and the slant culture medium both comprise the following components: 10g of peptone, 5g of beef extract, 5g of sodium chloride, 2g of yeast powder and 20g of agar, adding water to a constant volume of 1L, and adjusting the pH value to 7.2;
(3) compounding of mixed strains
Mixing the inoculation solutions of the strains to obtain a compound inoculation solution;
(4) preparation of first-order seed liquid
Inoculating the complex inoculum to a solid culture medium according to the inoculum size of 10% by volume, and culturing at the rotating speed of 220rpm of a shaking table and the temperature of 55 ℃ for 20h to obtain a first-stage seed solution;
(5) preparing a complex microbial inoculum:
inoculating the first-stage seed liquid into the fermentation culture medium according to the inoculation amount of 2% of the volume ratio, wherein the ventilation amount is 8m3H, the stirring speed is 220rpm, the culture is carried out for 20h at the temperature of 55 ℃, and the amount of viable bacteria in the liquid microbial inoculum is more than 1 multiplied by 1010When CFU/g is needed, the compound microbial inoculum is obtained;
wherein, the fermentation medium comprises the following components: 15g/L of brown sugar, 1.0g/L of ammonium sulfate, 3g/L of peptone, 2g/L of yeast powder and 5g/L of sodium chloride, adding water to a constant volume of 1L, and adjusting the pH value to 7.2.
Performance testing
Selecting a deodorizing workshop of a municipal sludge treatment plant in a certain city, wherein the air volume treated by the biological filter is 15000m3H, experiments were performed on average once a month. 1-5 parts of the composite microbial inoculum prepared in the examples 1-5 are respectively added and subjected to biological deodorization treatment through a biological filter, and the addition amount is 1.5 kg; the control group was prepared by deodorizing in a biofilter without adding complex microbial inoculum.
And detecting the content of ammonia gas, the content of hydrogen sulfide and the content of total VOCs in the waste gas before and after treatment respectively, and calculating the removal rate. The results are shown in Table 1.
TABLE 1 results of sewage treatment in examples 1 to 5 and control group
As can be seen from Table 1, the addition of the complex microbial agents of examples 1 to 5 can significantly reduce the ammonia gas content, the hydrogen sulfide content and the total VOCs content in the wastewater compared with the control group.
The experiments show that the composite microbial inoculum provided by the invention utilizes the functional microbial flora to absorb, decompose and convert odor molecules into odorless substances, inhibits the growth of odor-producing microorganisms through the antagonistic action of the microorganisms, and reduces the generation and release of new malodorous gases such as ammonia, hydrogen sulfide and the like in the biofiltration process, thereby achieving the purpose of eliminating or inhibiting the generation of odor; and the preparation is simple, the deodorization is rapid, the effect is durable, and no secondary pollution is caused.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. The composite microbial inoculum for efficiently deodorizing the biological filter is characterized by comprising the following raw materials in parts by weight: 50-70 parts of alcaligenes faecalis, 50-70 parts of saccharomyces cerevisiae, 30-60 parts of lactobacillus delbrueckii, 30-60 parts of bacillus subtilis, 15-25 parts of bacillus megaterium, 15-25 parts of thiobacillus denitrificans, 10-20 parts of thiobacillus oxydans and 10-20 parts of EM bacteria.
2. The composite microbial inoculum for efficiently deodorizing a biological filter according to claim 1, which is characterized by comprising the following raw materials in parts by weight: 55-65 parts of alcaligenes faecalis, 55-65 parts of saccharomyces cerevisiae, 40-50 parts of lactobacillus delbrueckii, 40-50 parts of bacillus subtilis, 18-22 parts of bacillus megaterium, 18-22 parts of thiobacillus denitrificans, 12-18 parts of thiobacillus oxydans and 12-18 parts of EM bacteria.
3. The composite microbial inoculum for efficiently deodorizing a biological filter according to claim 2, which is characterized by comprising the following raw materials in parts by weight: 60 parts of alcaligenes faecalis, 60 parts of saccharomyces cerevisiae, 45 parts of lactobacillus delbrueckii, 45 parts of bacillus subtilis, 20 parts of bacillus megaterium, 20 parts of thiobacillus denitrificans, 15 parts of thiobacillus oxydans and 15 parts of EM (effective microorganisms).
4. The composite bacterial agent for efficiently deodorizing a biofilter according to any one of claims 1 to 3, wherein the number of deposited Alcaligenes faecalis is: CGMCC No.5219, the preservation number of the saccharomyces cerevisiae is as follows: CGMCC No. 3217.
5. The composite bacterial agent for efficiently deodorizing a biofilter according to any one of claims 1 to 3, wherein the lactobacillus delbrueckii has a deposit number of: CGMCC No.15445, the preservation number of the Bacillus subtilis is as follows: CGMCC No. 3038.
6. The composite bacterial agent for efficiently deodorizing a biofilter according to any one of claims 1 to 3, wherein the Bacillus megaterium has the deposit number: GMCC No. 1259.
7. The complex microbial inoculum for efficiently deodorizing a biofilter according to any one of claims 1 to 3, wherein the deposited number of the denitrobacterium is as follows: CGMCC NO.11780, the preservation number of the thiobacillus thiooxidans is as follows: CGMCC No. 12387.
8. The composite bacterial agent for efficiently deodorizing a biofilter according to any one of claims 1 to 3, wherein the total effective viable count of the composite bacterial agent is more than or equal to 1 x 1010CFU/g。
9. A preparation method of a composite microbial inoculum for efficiently deodorizing a biological filter is characterized by comprising the following steps:
(1) weighing the raw materials
Weighing each strain according to the weight part of the composite microbial inoculum of any one of claims 1 to 8;
(2) strain activation
Respectively inoculating each strain to solid culture medium, culturing at 50-55 deg.C for 16-20h, selecting single colony to be inoculated to slant culture medium, culturing at 50-55 deg.C for 16-20h, washing with sterile water to obtain surface thallus as inoculation liquid with viable bacteria amount greater than 1 × 1010CFU/g；
(3) Compounding of mixed strains
Mixing the inoculation solutions of the strains to obtain a compound inoculation solution;
(4) preparation of first-order seed liquid
Inoculating the complex seed solution into the solid culture medium according to the inoculum size of 5-10% of the volume ratio, and culturing for 16-20h at the rotating speed of a shaking table of 180-220rpm and the temperature of 50-55 ℃ to obtain a first-stage seed solution;
(5) preparing a complex microbial inoculum:
inoculating the first-stage seed liquid into the fermentation culture medium according to the inoculation amount of 0.5-2% of the volume ratio, wherein the ventilation amount is 6-8m3H, stirring speed of 180-The viable bacteria content is more than 1 × 1010And when the concentration is CFU/g, the compound microbial inoculum is obtained.
10. The method for preparing composite bacterial agent for efficiently deodorizing a biofilter according to claim 9, wherein in the step (2), the composition of the solid culture medium and the composition of the slant culture medium are both as follows: 10g of peptone, 5g of beef extract, 5g of sodium chloride, 2g of yeast powder and 20g of agar, adding water to a constant volume of 1L, and adjusting the pH value to 7.2;
in the step (5), the fermentation medium comprises the following components: 15g/L of brown sugar, 1.0g/L of ammonium sulfate, 3g/L of peptone, 2g/L of yeast powder and 5g/L of sodium chloride, adding water to a constant volume of 1L, and adjusting the pH value to 7.2.
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