CN108934749A

CN108934749A - A kind of cultural method improving Pleurotus eryngii quality

Info

Publication number: CN108934749A
Application number: CN201810685954.9A
Authority: CN
Inventors: 谢宗宜
Original assignee: Jingxi Xiumei Biancheng Agricultural Science And Technology Co Ltd
Current assignee: Jingxi Xiumei Biancheng Agricultural Science And Technology Co Ltd
Priority date: 2018-06-28
Filing date: 2018-06-28
Publication date: 2018-12-07

Abstract

The present invention relates to field of edible fungus culture, and in particular to a kind of cultural method for improving Pleurotus eryngii quality, comprising the following steps: the first step, the preparation of culture bag；Second step, inoculation, bacterium germination；Third step takes off bag, spells bag, earthing；4th step removes flower bud；5th step, management of producing mushroom；6th step, harvesting.Present invention has the advantage that raw material can be reasonably selected according to local circumstance, increase raw material selectivity, reduce production cost, cultivation matrix nutrition is balanced and has the effect of anti-varied bacteria growing so as to increase the size of fructification, single mushroom fresh weight is 185-205g, and single mushroom shank diameter can reach 8-13cm.

Description

A kind of cultural method improving Pleurotus eryngii quality

[technical field]

The present invention relates to field of edible fungus culture, in particular to a kind of cultural method for improving Pleurotus eryngii quality.

[background technique]

China's Edible Fungi has had reached more than 18,800 ten thousand tons in 2009 by more than 30 years scales of developping production, Account for 70% or more of global total output, the yield of edible mushroom and produce at the forefront in the world to all positions, it has also become China after grain, cotton, The sixth-largest plantation industry after oil, dish, fruit.Pleurotus eryngii also known as pleurotus eryngii, because its fragrance and bacterial context with almond is plump As abalone mouthfeel and gain the name.Pleurotus eryngii bacterial context is plump, and quality is tender and crisp, especially stem dense structure, solid, milky white, can be complete Portion is edible, and stem cunning more crisp than cap, tasty and refreshing, referred to as " oyster mushroom king ", " dried scallop mushroom ", have pleasant almond flavor and Such as the mouthfeel of abalone, be suitble to fresh-keeping, processing, firmly get liking for people, be exploitation cultivation in recent years it is successful and edible, medicinal, Dietotherapy is in the Rare edible fungus new varieties of one.

Pleurotus eryngii mainly produces in a conventional manner at present, usually culture material and Pleurotus eryngii spore are planted using bag, bottle is planted or Case is planted, and this production cultural method is grown in relatively closed space due to being, therefore in the growth course of mycelia and thallus In, air circulation is poor, and moisture supply is uneven, and unstable so as to cause yield, space utilization rate is low, and by season, set The influence with environmental condition is applied, nutrient imbalance causes strain survival rate low, and mycelia is easy to aging, and strain is caused to make a variation, and influences the later period The growth of Pleurotus eryngii, trophic structure is unreasonable, causes the underproduction of Pleurotus eryngii, the serious consequences such as quality decline.

[summary of the invention]

In view of above content, the present inventor obtains a kind of cultural method for improving Pleurotus eryngii quality, raw material through many years research It can be reasonably selected according to local circumstance, increase raw material selectivity, reduce production cost, cultivation matrix nutrition equilibrium is simultaneously And having the effect of anti-varied bacteria growing so as to increase the size of fructification, single mushroom fresh weight is 185-205g, single mushroom Shank diameter can reach 8-13cm.

In order to achieve the above objectives, the technical scheme adopted by the invention is that:

A kind of cultural method improving Pleurotus eryngii quality, specifically includes the following steps:

The first step, the preparation of culture bag:

By weight, 20-30 parts of grouts are weighed, 10-20 parts of straw, 60-80 parts of grape stalk, 10-20 parts of litchi meat, 5-10 parts of egg shell, heap fermentation is built after crushing, the central temperature of the 1-3 days control fermentation materials of fermentation is 40-50 DEG C, 4-8 The central temperature of its control fermentation material is 60-70 DEG C, and the central temperature of the 9-10 days control fermentation materials is 60-80 DEG C, is sent out Add 15-25 parts of Flos Lonicerae extractive solution after ferment material, 15-20 parts of beans fruit extracting solution of leopard cat tail, 10-20 parts of shepherd's purse extracting solution, pea 15-20 parts of beans extracting solution, 15-25 parts of water, it is packed into culture bag after stirring, is placed in autoclave and sterilizes, afterwards certainly So it is cooled to room temperature；

Second step, inoculation, bacterium germination:

Sterilized culture bag is accessed into Pleurotus eryngii parent species, culture to mycelia under preference temperature is placed on and is covered with culture bag；

Third step takes off bag, spells bag, earthing:

Plastic film in culture bag is cut, is sloughed, is placed on the cultivation bed put in order in advance, the culture bag heap row At cuboid, the length of the cuboid connects the length formed by 3-5 culture bag from beginning to end, the width of the cuboid be by The length that 5-8 culture bag successively forms side by side along the long side direction, the height of the cuboid be by 3-5 culture bag successively by Up to lower tired heap and the horizontal accumulation along length cross section of tired heap face, the cuboid it is wide in two side by side culture bag border on Gap matching is provided with ventilation duct, and ventilation duct is arranged along the length direction of cuboid, and the length of the ventilation duct is greater than The length of cuboid extends the mouth two-port of ventilation duct outside cuboid；The mutual consolidation splicing of culture bag, every interlayer spray Binder solution, top layer cover the soil of 2-3cm, and sprinkle water and keep soil wet；

4th step removes flower bud:

When growing more Pleurotus eryngii mushroom flower bud wait cultivate bed, selects and retain healthy and strong mushroom flower bud, and retain two healthy and strong adjacent mushrooms The spacing of flower bud is 10 centimetres, extracts remaining mushroom flower bud, carries out within every 2 days once removing flower bud, until no longer long fruiting flower bud；

5th step, management of producing mushroom:

Mushroom flower bud grows up, and Pleurotus eryngii fructification length is after 3-5cm, the every 2-3 days sprinkling increase elements one on Pleurotus eryngii mushroom handle It is secondary, 2-3ml/ is sprayed every time only, it is long until after 10-15cm high until Pleurotus eryngii fructification length；Pleurotus eryngii fructification length In 8-13cm, mushroom covers the harvesting in 4-6cm

The temperature, humidity and intensity of illumination of mushroom shed are controlled out, by the way of water spray, ventilation, adjusting illumination in favor of apricot Abalone mushroom growth；

6th step, harvesting:

When Pleurotus eryngii fructification length it is long to 10-15cm after, fitted when open and flat, spore not yet launch by mushroom lid for harvesting Phase harvests in time when fructification bacteria cover diameter reaches 4-6cm；Culture bag continuation moisturizing, and same fruiting are given after harvesting first time The processing means of management are identical, until two damp mushrooms can be harvested.

It further illustrates, the Flos Lonicerae extractive solution is prepared by following methods: by weight, by dry gold 10-20 parts of honeysuckle flower, 70-80 DEG C of 20-30 parts of boiled water immersion 1-5 minutes is added, repeats to impregnate 3-5 times, the filter that will be filtered every time Liquid merging carry out heating be concentrated into 1.15 times of original volume after, use power for the supersonic oscillations 10-30S of 300-500W after, Standing filters to take supernatant, and supernatant is eluted by the ethanol solution that mass fraction is 75-80%, collects eluent, Obtain the Flos Lonicerae extractive solution of high-purity tea polypenols；

The leopard cat tail beans fruit extracting solution is prepared by following methods: by weight, by fresh leopard cat tail beans fruit 1-5 parts are added 10-30 part of water and carry out heating and boil to rotting, and filter to take filtrate, are subsequently added into pH as 1.0-2.0 equilibrium liquid Adjusting filtrate is acid filtrate, then adsorbs acid filtrate by being mounted with the ion column of cation exchange resin, connects Using mass fraction be 80-85% ethanol solution elution ionic column, collect eluent, obtain leopard cat tail beans fruit extracting solution.

It further illustrates, the shepherd's purse extracting solution is prepared by following methods: by weight, by fresh shepherd's purse 5-10 parts, 10-20 parts of water is added after cleaning and stirs into juice, is subsequently added into active carbon except depigmentaton, is subsequently added into mass fraction It extracts 10-15 hours for the ethanol solution of 80-85%, after evaporating ethanol solution, leaching liquor is inclined with flow velocity for 1-2ml/s It pours on the ion exchange column wall for be mounted with macroporous absorbent resin and is adsorbed, with mass fraction be again then 75-80%'s Ethanol solution is eluted, and shepherd's purse extracting solution can be obtained in the eluent evaporating ethanol solution after elution；

The pea extracting solution is prepared by following methods: by weight, by fresh 5-10 parts of pea benevolence, It stirs and is slurried after 5-10 part water are added, being subsequently added into 20-50 parts of concentration is alcohol solution dipping 3-8 hours of 75-85%, It is then refluxed for extracting, extract 3-5 times, extract 0.2-0.3 hours every time, merge the filtrate extracted every time, obtain pea extracting solution.

It further illustrates, described adhesive solution is prepared by following methods: by weight, club fungi being mentioned Take 10-20 parts of liquid, 8-15 parts of red Phallus extracting solution, 5-8 parts of maltodextrin, 3-8 parts of poly-aspartate, tea polyphenols 0.2-0.5 Part, 3-8 parts of sucrose, 0.2-0.5 parts of vitamin B1,0.2-0.5 parts of riboflavin, 0.1-0.2 parts of potassium fulvate, ammonium metaphosphate 0.2-0.5 parts, 0.2-0.5 parts of EDTA- iron, 0.2-0.4 parts of EDTA- zinc, 0.1-0.2 parts of EDTA- calcium, EDTA- magnesium 0.2-0.3 Part, it 50-60 parts of water, stirs evenly obtained；

The club fungi extracting solution is prepared by following methods: by weight, by fresh club fungi 10-15 Part, 100-200 parts of water, it is broken into homogenate after mixing, is extracted 0.2-0.5 hours in 38-40 DEG C of water-bath, it is auxiliary during extraction Assist rate is the ultrasonic wave of 100-200W, and then layer filtered through gauze obtains club fungi extracting solution repeatedly；

The red Phallus extracting solution is prepared by following methods: by weight, will new scarlet Phallus 10-20 Part, juice is stirred into after 50-100 parts of water mixing, is extracted 0.3-0.6 hours in 38-40 DEG C of water-bath, auxiliary power is during extraction The ultrasonic wave of 100-200W then obtains red Phallus extracting solution using three layers of filtered through gauze.

It further illustrates, the increase element is to prepare according to the following formulation: by weight, by 2,4-DNP sodium It 0.2-0.5 parts, 0.1-0.3 parts of benzimidazole, 0.5-1 parts of amine fresh fat, 0.1-0.3 parts of zeatin, is dissolved in 3-5 parts of alcohol, 0.5-1 parts of Tween-80s are added, 2000-3000 parts of water stir evenly obtained.

It further illustrates, the diameter of the ventilation duct is 1.0cm, and multiple ventholes are offered on tube wall.

It further illustrates, the Pleurotus eryngii selects middle-late ripening variety.

It further illustrates, the culture bag is cylinder；The specification of culture bag: long 40-50cm, directly through 8-10cm.

It further illustrates, the temperature of the mushroom shed out is controlled in 12-15 DEG C, humid control in 80-90% and intensity of illumination Control is in the lux 600-700.

In conclusion by adopting the above-described technical solution, the beneficial effects of the present invention are:

1, the present invention replaces after having selected the primary raw materials such as grouts, straw, grape stalk, litchi meat, egg shell to ferment The segment wood cultivated of the prior art is changed, therefore, the selectivity of raw material is significantly increased, can adaptation to local conditions selection agricultural by product, leftover bits and pieces Material or waste material reduce production cost as raw material.It is easy to the adjusting of nutritional ingredient simultaneously, is conducive to mycelia fast-growth；It reduces Pleurotus eryngii industry sustainable development is realized in the segment wood cultivated felling to forest tree resource；And the present invention adopts in the course of cultivation With de- bag and bag operation is spelled, de- bag operation can be such that mycelium directly contacts with external environment, utilize oxygen, moisture and nutrition The exchange of substance, spelling bag operation make mutually close connection between each bacterium bag, and the hyphal cell of bacterium bag junction, which passes through, mutually to be merged, It allows mycelia metabolic nutrition object to be able to mutually transport between bacterium bag, transmit, spliced dozens of bacterium bag is made to be linked to be an entirety, So that mycelia metabolic nutrition object total amount needed for having segment wood cultivated Pleurotus eryngii, sufficient nutrition is provided for Growth of Pleurotus eryngii Condition.Adaptation to local conditions grouts of the present invention, the raw material of straw, grape stalk, litchi meat, egg shell as culture substrate, it is not only right Local resources have carried out effective comprehensive utilization, and the raw material of selection is degradable biomass material, generated agriculture waste Object will not bring burden to environment.By being mixed after grouts, straw, grape stalk, litchi meat, egg shell fermentation plus extracting solution The culture substrate physicochemical property index obtained after material is suitable for the cultivation of Pleurotus eryngii, and abundance can be provided for the growth of Pleurotus eryngii Nutrient source effectively improves Pleurotus eryngii yield and quality.

2, it is Pleurotus eryngii generation material that the present invention, which has selected honeysuckle, leopard cat tail beans fruit, the extracting solution of shepherd's purse and pea extracting solution, The constituent of culture medium can effectively reduce the miscellaneous bacteria infection rate of Pleurotus eryngii substituting stuff cultivation using above-mentioned extracting solution, and will not The mycelia growing of pleurotus eryngii and button formation are damaged.

3, binder solution used in the present invention mainly contains club fungi extracting solution, red Phallus extracting solution, malt paste The Multiple components such as essence, poly-aspartate, tea polyphenols, the binder solution prepared using said components, can be such that hyphal cell merges Time foreshortens to 5 hours, and time of fusion shortens more than 4 times than the test process of unused binder solution, moreover it is possible to improve Pleurotus eryngii The content of middle polysaccharide and crude protein.

Therefore, Pleurotus eryngii method of the invention has raw material selectively strong compared with existing segment wood cultivated Pleurotus eryngii, raw Produce at low cost, mycelia growth is fast, and effective component is high, the low technical advantage of miscellaneous bacteria infection rate.

[Detailed description of the invention]

Fig. 1 is that culture bag of the present invention is spelled after bag and the positional structure schematic diagram of ventilation duct；

Appended drawing reference: 1- cultivating bag, 2- snorkel.

[specific embodiment]

In order to make those skilled in the art more fully understand technical solution of the present invention, the present invention is retouched in detail below State, the description of this part be only it is exemplary and explanatory, should not have any restriction effect to protection scope of the present invention.

Embodiment 1:

One, raw material early-stage preparations:

1. Flos Lonicerae extractive solution is prepared by following methods: by weight, dry 10 parts of honeysuckle being added 20 parts of boiled water of 70 DEG C impregnate 1 minute, repeat to impregnate 3 times, and the filtrate filtered every time merging is carried out heating and is concentrated into original volume 1.15 times after, use power for the supersonic oscillations 10S of 300W after, standing filter to take supernatant, supernatant is passed through into quality The ethanol solution that score is 75% is eluted, and is collected eluent, is obtained the Flos Lonicerae extractive solution of high-purity tea polypenols.

2. leopard cat tail beans fruit extracting solution is prepared by following methods: by weight, by fresh 1 part of leopard cat tail beans fruit It 10 parts of water is added carries out heating and boil to rotting, filter to take filtrate, being subsequently added into pH is that adjust filtrate be acid to 1.0 equilibrium liquids Property filtrate, acid filtrate is adsorbed by being mounted with the ion column of cation exchange resin then, then uses quality point The ethanol solution elution ionic column that number is 80% collects eluent, obtains leopard cat tail beans fruit extracting solution.

3. shepherd's purse extracting solution is prepared by following methods: by weight, 5 parts of fresh shepherd's purse adding after cleaning Enter 10 parts of water and stir into juice, is subsequently added into active carbon except depigmentaton, is subsequently added into the ethanol solution that mass fraction is 80% and soaks Mention 10 hours, after evaporating ethanol solution, by leaching liquor with flow velocity be 1ml/s pour into be mounted with macroporous absorbent resin from It is adsorbed, is then eluted again with the ethanol solution that mass fraction is 75%, the elution after elution on son exchange column wall Liquid evaporating ethanol solution, can be obtained shepherd's purse extracting solution.

4. pea extracting solution is prepared by following methods: by weight, by fresh 5 parts of pea benevolence, being added 5 It stirs and is slurried after part water, the alcohol solution dipping hour that the concentration for being subsequently added into 20 parts is 75%, be then refluxed for extracting, extract 3 It is secondary, it extracts 0.2 hour every time, merges the filtrate extracted every time, obtain pea extracting solution.

5. binder solution is prepared by following methods: by weight, by 10 parts of club fungi extracting solution, red ghost 8 parts of extracting solution, 5 parts of maltodextrin, 3 parts of poly-aspartate, 0.2 part of tea polyphenols, 3 parts of sucrose, 0.2 part of vitamin B1, core 0.2 part of flavine, 0.1 part of potassium fulvate, 0.2 part of ammonium metaphosphate, 0.2 part of EDTA- iron, 0.2 part of EDTA- zinc, EDTA- calcium 0.1 Part, it 0.2 part of EDTA- magnesium, 50 parts of water, stirs evenly obtained；

The club fungi extracting solution is prepared by following methods: by weight, by 10 parts of fresh club fungi, water 100 parts, it is broken into homogenate after mixing, is extracted 0.2 hour in 38 DEG C of water-baths, auxiliary power is the super of 100W during extraction Sound wave, then layer filtered through gauze obtains club fungi extracting solution repeatedly；

The red Phallus extracting solution is prepared by following methods: by weight, by new 10 parts of scarlet Phallus, water Juice is stirred into after 50 parts of mixing, is extracted 0.3 hour in 38 DEG C of water-baths, auxiliary power is the ultrasonic wave of 100W during extraction, then Red Phallus extracting solution is obtained using three layers of filtered through gauze.

6. increasing element is to prepare according to the following formulation: by weight, by 0.2 part of 2,4-DNP sodium, benzimidazole It 0.1 part, 0.5 part of amine fresh fat, 0.1 part of zeatin, is dissolved in 3 parts of alcohol, adds 0.5 part of Tween-80,2000 parts of water stir It mixes and is uniformly made.

The substance that above-mentioned early period is prepared is used for following cultural methods for improving Pleurotus eryngii quality.

2. a kind of cultural method for improving Pleurotus eryngii quality, specifically includes the following steps:

The first step, the preparation of culture bag:

By weight, 30 parts of grouts are weighed, 20 parts of straw, 80 parts of grape stalk, 20 parts of litchi meat, 10 parts of egg shell, Heap fermentation is built after crushing, the central temperature of the 3rd day control fermentation material of fermentation is 50 DEG C, the center temperature of the 8th day control fermentation material Degree is 70 DEG C, and the central temperature of the 10th day control fermentation material is 80 DEG C, adds Flos Lonicerae extractive solution 25 after obtaining fermentation material Part, 20 parts of fruit extracting solution of leopard cat tail beans, 10 parts of shepherd's purse extracting solution, 20 parts of pea extracting solution, 25 parts of water, after stirring Loading cylinder and long 50cm is placed in autoclave and sterilizes directly through the culture bag of 10cm, rear cooled to room temperature；

Second step, inoculation, bacterium germination:

By Japanese No. 5 parent species of sterilized culture bag access Pleurotus eryngii, it is placed on culture to mycelia under preference temperature and is covered with Culture bag；

Third step takes off bag, spells bag, earthing:

Plastic film in culture bag is cut, is sloughed, is placed on the cultivation bed put in order in advance, the culture bag heap row At cuboid, the length of the cuboid connects the length formed by 5 culture bags from beginning to end, and the wide of the cuboid is by 8 The length that culture bag successively forms side by side along the long side direction, the height of the cuboid are successively from top to bottom tired out by 5 culture bags The heap and tired heap face horizontal accumulation along length cross section, the cuboid it is wide in two side by side culture bag border on gap It is 1.0cm with diameter is provided with, and offers the ventilation duct of multiple ventholes on tube wall, and ventilation duct is along the length of cuboid Direction setting is spent, the length of the ventilation duct is greater than the length of cuboid, the mouth two-port of ventilation duct is made to extend cuboid Outside；The mutual consolidation splicing of culture bag, every interlayer spray binder solution, and top layer covers the soil of 3cm, and sprinkles water and keep mud Soil is wet；

4th step removes flower bud:

5th step, management of producing mushroom:

Mushroom flower bud grows up, and Pleurotus eryngii fructification length is sprayed on Pleurotus eryngii mushroom handle after 5cm and increase element once, often for every 3 days It is secondary to spray 3ml/ only, it is long until after 15cm high until Pleurotus eryngii fructification length；Pleurotus eryngii fructification length is in 13cm, mushroom Cover the harvesting in 6cm

The temperature of mushroom shed is controlled out in 15 DEG C, humidity 90% and intensity of illumination by the way of water spray, ventilation, adjusting illumination 700 luxs, in favor of Growth of Pleurotus eryngii；

6th step, harvesting:

When Pleurotus eryngii fructification length it is long to 15cm after, be Harvesting Date when open and flat, spore not yet launch by mushroom lid, It is harvested in time when fructification bacteria cover diameter reaches 6cm；Culture bag continuation moisturizing, and same management of producing mushroom are given after harvesting first time Processing means it is identical, until two damp mushrooms can be harvested.

Embodiment 2:

1, raw material early-stage preparations:

1. Flos Lonicerae extractive solution is prepared by following methods: by weight, dry 20 parts of honeysuckle being added 30 parts of boiled water of 80 DEG C impregnate 5 minutes, repeat to impregnate 5 times, and the filtrate filtered every time merging is carried out heating and is concentrated into original volume 1.15 times after, use power for the supersonic oscillations 30S of 500W after, standing filter to take supernatant, supernatant is passed through into quality The ethanol solution that score is 80% is eluted, and is collected eluent, is obtained the Flos Lonicerae extractive solution of high-purity tea polypenols.

2. leopard cat tail beans fruit extracting solution is prepared by following methods: by weight, by fresh 5 parts of leopard cat tail beans fruit It 30 parts of water is added carries out heating and boil to rotting, filter to take filtrate, being subsequently added into pH is that adjust filtrate be acid to 2.0 equilibrium liquids Property filtrate, acid filtrate is adsorbed by being mounted with the ion column of cation exchange resin then, then uses quality point The ethanol solution elution ionic column that number is 85% collects eluent, obtains leopard cat tail beans fruit extracting solution.

3. shepherd's purse extracting solution is prepared by following methods: by weight, 10 parts of fresh shepherd's purse adding after cleaning Enter 20 parts of water and stir into juice, is subsequently added into active carbon except depigmentaton, is subsequently added into the ethanol solution that mass fraction is 85% and soaks Mention 15 hours, after evaporating ethanol solution, by leaching liquor with flow velocity be 2ml/s pour into be mounted with macroporous absorbent resin from It is adsorbed, is then eluted again with the ethanol solution that mass fraction is 80%, the elution after elution on son exchange column wall Liquid evaporating ethanol solution, can be obtained shepherd's purse extracting solution.

4. pea extracting solution is prepared by following methods: by weight, fresh 10 parts of pea benevolence being added It stirs and is slurried after 10 parts of water, being subsequently added into 50 parts of concentration is alcohol solution dipping 8 hours of 85%, is then refluxed for extracting, mention It takes 5 times, extracts 0.3 hour every time, merge the filtrate extracted every time, obtain pea extracting solution.

5. binder solution is prepared by following methods: by weight, by 20 parts of club fungi extracting solution, red ghost 15 parts of extracting solution, 8 parts of maltodextrin, 8 parts of poly-aspartate, 0.5 part of tea polyphenols, 8 parts of sucrose, 0.5 part of vitamin B1, 0.5 part of riboflavin, 0.2 part of potassium fulvate, 0.5 part of ammonium metaphosphate, 0.5 part of EDTA- iron, 0.4 part of EDTA- zinc, EDTA- calcium 0.2 Part, it 0.3 part of EDTA- magnesium, 60 parts of water, stirs evenly obtained；

The club fungi extracting solution is prepared by following methods: by weight, by 15 parts of fresh club fungi, water 200 parts, it is broken into homogenate after mixing, is extracted 0.5 hour in 40 DEG C of water-baths, auxiliary power is the super of 200W during extraction Sound wave, then layer filtered through gauze obtains club fungi extracting solution repeatedly；

The red Phallus extracting solution is prepared by following methods: by weight, by new 20 parts of scarlet Phallus, water Juice is stirred into after 100 parts of mixing, is extracted 0.6 hour in 40 DEG C of water-baths, auxiliary power is the ultrasonic wave of 200W during extraction, is connect Obtain red Phallus extracting solutions using three layers of filtered through gauze.

6. increasing element is to prepare according to the following formulation: by weight, by 0.5 part of 2,4-DNP sodium, benzimidazole It 0.3 part, 1 part of amine fresh fat, 0.3 part of zeatin, is dissolved in 5 parts of alcohol, adds 1 part of Tween-80,3000 parts of water, stirring is It is even to be made.

The first step, the preparation of culture bag:

By weight, 24 parts of grouts are weighed, 13 parts of straw, 68 parts of grape stalk, 15 parts of litchi meat, 7 parts of egg shell, Heap fermentation is built after crushing, the central temperature of the 2nd day control fermentation material of fermentation is 45 DEG C, the center temperature of the 5th day control fermentation material Degree is 65 DEG C, and the central temperature of the 10th day control fermentation material is 65 DEG C, adds Flos Lonicerae extractive solution 18 after obtaining fermentation material Part, 17 parts of fruit extracting solution of leopard cat tail beans, 14 parts of shepherd's purse extracting solution, 18 parts of pea extracting solution, 20 parts of water, after stirring Loading cylinder and long 44cm is placed in autoclave and sterilizes directly through the culture bag of 9cm, rear cooled to room temperature；

Second step, inoculation, bacterium germination:

Sterilized culture bag is accessed into the parent species of Pleurotus eryngii Beijing 4, culture to mycelia under preference temperature is placed on and is covered with Culture bag；

Third step takes off bag, spells bag, earthing:

Plastic film in culture bag is cut, is sloughed, is placed on the cultivation bed put in order in advance, the culture bag heap row At cuboid, the length of the cuboid connects the length formed by 4 culture bags from beginning to end, and the wide of the cuboid is by 7 The length that culture bag successively forms side by side along the long side direction, the height of the cuboid are successively from top to bottom tired out by 4 culture bags The heap and tired heap face horizontal accumulation along length cross section, the cuboid it is wide in two side by side culture bag border on gap It is 1.0cm with diameter is provided with, and offers the ventilation duct of multiple ventholes on tube wall, and ventilation duct is along the length of cuboid Direction setting is spent, the length of the ventilation duct is greater than the length of cuboid, the mouth two-port of ventilation duct is made to extend cuboid Outside；The mutual consolidation splicing of culture bag, every interlayer spray binder solution, and top layer covers the soil of 2.5cm, and holding of sprinkling water Soil is wet；

4th step removes flower bud:

5th step, management of producing mushroom:

Mushroom flower bud grows up, and Pleurotus eryngii fructification length is sprayed on Pleurotus eryngii mushroom handle after 3.5cm and increase element once for every 2 days, 2.5ml/ is sprayed every time only, it is long until after 12cm high until Pleurotus eryngii fructification length；Pleurotus eryngii fructification length exists 10cm, mushroom cover the harvesting in 4.5cm

The temperature of mushroom shed is controlled out in 13 DEG C, humidity 85% and intensity of illumination by the way of water spray, ventilation, adjusting illumination 650 luxs, in favor of Growth of Pleurotus eryngii；

6th step, harvesting:

When Pleurotus eryngii fructification length it is long to 12cm after, be Harvesting Date when open and flat, spore not yet launch by mushroom lid, It is harvested in time when fructification bacteria cover diameter reaches 4.5cm；Culture bag continuation moisturizing is given after harvesting first time, and with fruiting pipe The processing means of reason are identical, until two damp mushrooms can be harvested.

Embodiment 3:

1, raw material early-stage preparations:

1. Flos Lonicerae extractive solution is prepared by following methods: by weight, dry 13 parts of honeysuckle being added 25 parts of boiled water of 75 DEG C impregnate 2 minutes, repeat to impregnate 4 times, and the filtrate filtered every time merging is carried out heating and is concentrated into original volume 1.15 times after, use power for the supersonic oscillations 18S of 350W after, standing filter to take supernatant, supernatant is passed through into quality The ethanol solution that score is 78% is eluted, and is collected eluent, is obtained the Flos Lonicerae extractive solution of high-purity tea polypenols.

2. leopard cat tail beans fruit extracting solution is prepared by following methods: by weight, by fresh 3 parts of leopard cat tail beans fruit It 20 parts of water is added carries out heating and boil to rotting, filter to take filtrate, being subsequently added into pH is that adjust filtrate be acid to 1.5 equilibrium liquids Property filtrate, acid filtrate is adsorbed by being mounted with the ion column of cation exchange resin then, then uses quality point The ethanol solution elution ionic column that number is 83% collects eluent, obtains leopard cat tail beans fruit extracting solution.

3. shepherd's purse extracting solution is prepared by following methods: by weight, 8 parts of fresh shepherd's purse adding after cleaning Enter 13 parts of water and stir into juice, is subsequently added into active carbon except depigmentaton, is subsequently added into the ethanol solution that mass fraction is 82% and soaks It mentions 12 hours, after evaporating ethanol solution, leaching liquor is poured into flow velocity for 1.5ml/s and is mounted with macroporous absorbent resin It is adsorbed, is then eluted again with the ethanol solution that mass fraction is 78%, washing after elution on ion exchange column wall De- liquid evaporating ethanol solution, can be obtained shepherd's purse extracting solution.

4. pea extracting solution is prepared by following methods: by weight, by fresh 6 parts of pea benevolence, being added 6 It stirs and is slurried after part water, being subsequently added into 30 parts of concentration is alcohol solution dipping 5 hours of 80%, is then refluxed for extracting, extract It 3.5 times, extracts 0.25 hour every time, merges the filtrate extracted every time, obtain pea extracting solution.

5. binder solution is prepared by following methods: by weight, by 15 parts of club fungi extracting solution, red ghost 10 parts of extracting solution, 7 parts of maltodextrin, 5 parts of poly-aspartate, 0.3 part of tea polyphenols, 5 parts of sucrose, 0.3 part of vitamin B1, 0.3 part of riboflavin, 0.15 part of potassium fulvate, 0.3 part of ammonium metaphosphate, 0.3 part of EDTA- iron, 0.25 part of EDTA- zinc, EDTA- calcium It 0.15 part, 0.25 part of EDTA- magnesium, 55 parts of water, stirs evenly obtained；

The club fungi extracting solution is prepared by following methods: by weight, by 12 parts of fresh club fungi, water 150 parts, it is broken into homogenate after mixing, is extracted 0.3 hour in 39 DEG C of water-baths, auxiliary power is the super of 150W during extraction Sound wave, then layer filtered through gauze obtains club fungi extracting solution repeatedly；

The red Phallus extracting solution is prepared by following methods: by weight, by new 14 parts of scarlet Phallus, water Juice is stirred into after 60 parts of mixing, is extracted 0.4 hour in 39 DEG C of water-baths, auxiliary power is the ultrasonic wave of 150W during extraction, then Red Phallus extracting solution is obtained using three layers of filtered through gauze.

6. increasing element is to prepare according to the following formulation: by weight, by 0.3 part of 2,4-DNP sodium, benzimidazole It 0.2 part, 0.7 part of amine fresh fat, 0.25 part of zeatin, is dissolved in 3.5 parts of alcohol, adds 0.7 part of Tween-80,2500 parts Water stirs evenly obtained.

The first step, the preparation of culture bag:

By weight, 30 parts of grouts are weighed, 20 parts of straw, 80 parts of grape stalk, 20 parts of litchi meat, 10 parts of egg shell, Heap fermentation is built after crushing, the central temperature of the 3rd day control fermentation material of fermentation is 50 DEG C, the center temperature of the 8th day control fermentation material Degree is 70 DEG C, and the central temperature of the 10th day control fermentation material is 80 DEG C, adds Flos Lonicerae extractive solution 25 after obtaining fermentation material Part, 20 parts of fruit extracting solution of leopard cat tail beans, 20 parts of shepherd's purse extracting solution, 20 parts of pea extracting solution, 25 parts of water, after stirring Loading cylinder and long 50cm is placed in autoclave and sterilizes directly through the culture bag of 10cm, rear cooled to room temperature；

Second step, inoculation, bacterium germination:

Sterilized culture bag is accessed into the parent species of Pleurotus eryngii Henan 7, culture to mycelia under preference temperature is placed on and is covered with Culture bag；

Third step takes off bag, spells bag, earthing:

Plastic film in culture bag is cut, is sloughed, is placed on the cultivation bed put in order in advance, the culture bag heap row At cuboid, the length of the cuboid connects the length formed by 3 culture bags from beginning to end, and the wide of the cuboid is by 6 The length that culture bag successively forms side by side along the long side direction, the height of the cuboid are successively from top to bottom tired out by 4 culture bags The heap and tired heap face horizontal accumulation along length cross section, the cuboid it is wide in two side by side culture bag border on gap It is 1.0cm with diameter is provided with, and offers the ventilation duct of multiple ventholes on tube wall, and ventilation duct is along the length of cuboid Direction setting is spent, the length of the ventilation duct is greater than the length of cuboid, the mouth two-port of ventilation duct is made to extend cuboid Outside；The mutual consolidation splicing of culture bag, every interlayer spray binder solution, and top layer covers the soil of 3cm, and sprinkles water and keep mud Soil is wet；

4th step removes flower bud:

5th step, management of producing mushroom:

6th step, harvesting:

Embodiment 4:

1, raw material early-stage preparations:

1. Flos Lonicerae extractive solution is prepared by following methods: by weight, dry 18 parts of honeysuckle being added 28 parts of boiled water of 79 DEG C impregnate 4 minutes, repeat to impregnate 4 times, and the filtrate filtered every time merging is carried out heating and is concentrated into original volume 1.15 times after, use power for the supersonic oscillations 25S of 450W after, standing filter to take supernatant, supernatant is passed through into quality The ethanol solution that score is 78% is eluted, and is collected eluent, is obtained the Flos Lonicerae extractive solution of high-purity tea polypenols.

2. leopard cat tail beans fruit extracting solution is prepared by following methods: by weight, by fresh 4 parts of leopard cat tail beans fruit It 25 parts of water is added carries out heating and boil to rotting, filter to take filtrate, being subsequently added into pH is that adjust filtrate be acid to 1.5 equilibrium liquids Property filtrate, acid filtrate is adsorbed by being mounted with the ion column of cation exchange resin then, then uses quality point The ethanol solution elution ionic column that number is 84% collects eluent, obtains leopard cat tail beans fruit extracting solution.

3. shepherd's purse extracting solution is prepared by following methods: by weight, 9 parts of fresh shepherd's purse adding after cleaning Enter 18 parts of water and stir into juice, is subsequently added into active carbon except depigmentaton, is subsequently added into the ethanol solution that mass fraction is 84% and soaks It mentions 14 hours, after evaporating ethanol solution, leaching liquor is poured into flow velocity for 1.8ml/s and is mounted with macroporous absorbent resin It is adsorbed, is then eluted again with the ethanol solution that mass fraction is 79%, washing after elution on ion exchange column wall De- liquid evaporating ethanol solution, can be obtained shepherd's purse extracting solution.

4. pea extracting solution is prepared by following methods: by weight, by fresh 9 parts of pea benevolence, being added 8 It stirs and is slurried after part water, being subsequently added into 45 parts of concentration is alcohol solution dipping 7 hours of 82%, is then refluxed for extracting, extract It 4.5 times, extracts 0.8 hour every time, merges the filtrate extracted every time, obtain pea extracting solution.

5. binder solution is prepared by following methods: by weight, by 18 parts of club fungi extracting solution, red ghost 12 parts of extracting solution, 7 parts of maltodextrin, 7 parts of poly-aspartate, 0.45 part of tea polyphenols, 7 parts of sucrose, 0.4 part of vitamin B1, 0.4 part of riboflavin, 0.18 part of potassium fulvate, 0.4 part of ammonium metaphosphate, 0.4 part of EDTA- iron, 0.35 part of EDTA- zinc, EDTA- calcium It 0.18 part, 0.28 part of EDTA- magnesium, 58 parts of water, stirs evenly obtained；

The club fungi extracting solution is prepared by following methods: by weight, by 14 parts of fresh club fungi, water 185 parts, it is broken into homogenate after mixing, is extracted 0.45 hour in 39 DEG C of water-baths, auxiliary power is the super of 190W during extraction Sound wave, then layer filtered through gauze obtains club fungi extracting solution repeatedly；

The red Phallus extracting solution is prepared by following methods: by weight, by new 18 parts of scarlet Phallus, water Juice is stirred into after 90 parts of mixing, is extracted 0.55 hour in 38 DEG C of water-baths, auxiliary power is the ultrasonic wave of 185W during extraction, is connect Obtain red Phallus extracting solutions using three layers of filtered through gauze.

6. increasing element is to prepare according to the following formulation: by weight, by 0.4 part of 2,4-DNP sodium, benzimidazole It 0.25 part, 0.95 part of amine fresh fat, 0.18 part of zeatin, is dissolved in 4.5 parts of alcohol, adds 0.8 part of Tween-80,2850 parts Water stirs evenly obtained.

The first step, the preparation of culture bag:

By weight, 28 parts of grouts are weighed, 19 parts of straw, 76 parts of grape stalk, 19 parts of litchi meat, 8 parts of egg shell, Heap fermentation is built after crushing, the central temperature of the 2nd day control fermentation material of fermentation is 48 DEG C, the center temperature of the 7th day control fermentation material Degree is 68 DEG C, and the central temperature of the 9th day control fermentation material is 78 DEG C, adds 23 parts of Flos Lonicerae extractive solution after obtaining fermentation material, It 19 parts of fruit extracting solution of leopard cat tail beans, 18 parts of shepherd's purse extracting solution, 19 parts of pea extracting solution, 22 parts of water, is packed into after stirring Cylindrical and long 49cm is placed in autoclave and sterilizes directly through the culture bag of 9cm, rear cooled to room temperature；

Second step, inoculation, bacterium germination:

Sterilized culture bag is accessed into the parent species of Pleurotus eryngii Fujian 2, culture to mycelia under preference temperature is placed on and is covered with Culture bag；

Third step takes off bag, spells bag, earthing:

4th step removes flower bud:

5th step, management of producing mushroom:

6th step, harvesting:

Verification test:

Test one:

The technical effect of 1 planting almond abalone mushroom of embodiment to illustrate the invention, the cultivation bacterium of the market buying of the weight such as selection Bag compares test, calculates compost production cost, and measurement Pleurotus eryngii mycelia covers with culture bag required time, bacterium bag miscellaneous bacteria Infection rate, average single mushroom bacteria cover diameter and average single mushroom fresh weight, specific data are as follows:

1 Pleurotus eryngii substituting stuff cultivation of table and segment wood cultivated cost and each growth indexes contrast table

From upper 1 data of table it is found that the compost production cost of Pleurotus eryngii substituting stuff cultivation of the invention and existing culture material Cost be not much different；The cultivation matrix of 1 culture bag of embodiment is obviously cultivated fastly with comparison the time required to covering with, and is shortened 17 days, significantly lower than the bacterium bag miscellaneous bacteria infection rate of comparison cultivation on bacterium bag miscellaneous bacteria infection rate；Pleurotus eryngii substituting stuff cultivation is put down Single mushroom bacteria cover diameter, average single mushroom fresh weight are apparently higher than comparison cultivation, and gap is significant.

Raw material of the present invention can adaptation to local conditions select agricultural by product, tailing, waste material, source is wide, passes through Plus the culture substrate physicochemical property obtained after extracting solution spice after grouts, straw, grape stalk, litchi meat, egg shell fermentation Index is suitable for the cultivation of Pleurotus eryngii, can provide sufficient nutrient source for the growth of Pleurotus eryngii, effectively improve Pleurotus eryngii yield And quality；Carbon-nitrogen ratio reaches between 34.5-41.7 after raw material cooperates, and is suitble to pleurotus eryngii fruiting, and Guangxi lichee in this year Stagnate to pin, five dimes it is wholesale it is all wholesale do not go out, cultivation base of the litchi fruits in positive good utilisation orchard as edible mushroom Matter finds that the polyoses content of the Pleurotus eryngii planted increases, and the fresh and sweet taste with lichee, during the fermentation sufficiently The sugar in litchi pulp is discharged, the time required to shortening fermentation as fermenting carbon source；But due to carbon source after straw or like vegetable fermentation Content height is perishable, is easy bonding, and moisture is easy to go out to will cause ponding problem into hardly possible, and egg shell will not be complete in fermentation process Full corrosion, only discharges the calcium ion being rich in eggshell, and uncorroded egg shell can improve the gas permeability of culture material and hydrophobic Property.

Test two:

Comparative example 1: substantially the same manner as Example 2, difference is: Flos Lonicerae extractive solution is not added in culture medium.

Comparative example 2: substantially the same manner as Example 2, difference is: leopard cat tail beans fruit extracting solution is not added in culture medium.

Comparative example 3: substantially the same manner as Example 2, difference is: shepherd's purse extracting solution is not added in culture medium.

Comparative example 4: substantially the same manner as Example 2, difference is: pea extracting solution is not added in culture medium.

Comparative example 5: substantially the same manner as Example 2, difference is: Flos Lonicerae extractive solution, leopard cat tail beans are not added in culture medium Fruit extracting solution.

Comparative example 6: substantially the same manner as Example 2, difference is: shepherd's purse extracting solution is not added in culture medium, pea extracts Liquid.

The bacterium bag miscellaneous bacteria infection rate of each processing is measured respectively, and observes mushroom flower bud growing state, and specific test data is as follows:

2 honeysuckle of table, leopard cat tail beans fruit, the extracting solution of shepherd's purse and pea extracting solution technical effect test data table

Test process	Bacterium bag miscellaneous bacteria infection rate	The growth of mushroom flower bud	Ca(mg/g)	Zn(mg/g)
					Embodiment 2	0.31%	It is powerful	0.584	0.087
Comparative example 1	15.21%	It is powerful	0.487	0.064
					Comparative example 2	11.64%	It is powerful	0.425	0.069
Comparative example 3	1.05%	It is more powerful	0.215	0.038
					Comparative example 4	1.58%	It is more powerful	0.224	0.036
Comparative example 5	22.58%	It is powerful	0.403	0.051
					Comparative example 6	3.57%	It is faint	0.201	0.032

It is Pleurotus eryngii generation material training that the present invention, which has selected honeysuckle, leopard cat tail beans fruit, the extracting solution of shepherd's purse and pea extracting solution, The constituent of base is supported, there is Flos Lonicerae extractive solution orientation to inhibit bacterial growth effect, and it is raw not influence Pleurotus eryngii mycelium It is long, but its Fungicidal substance is unstable, and leopard cat tail beans fruit extracting solution has a variety of alkaloids, can delay honeysuckle antipathogenic composition Degradation speed promotes the sterilization effectiveness of Flos Lonicerae extractive solution, but leopard cat tail beans fruit extracting solution is formed with inhibition to Pleurotus eryngii button Effect, inventor has found that shepherd's purse extracting solution and pea extracting solution can be added, can promote the growth of Pleurotus eryngii button, Shepherd's purse extracting solution can be in conjunction with Pleurotus eryngii mycelia, the influence that maskable leopard cat tail beans fruit extracting solution grows Pleurotus eryngii mushroom flower bud, pea Beans extracting solution has synergistic effect in conjunction with shepherd's purse extracting solution, to shepherd's purse extracting solution.It can effectively reduce using above-mentioned extracting solution The miscellaneous bacteria infection rate of Pleurotus eryngii substituting stuff cultivation, and the mycelia growing of pleurotus eryngii and button formation will not be damaged.It can by table 3 Know, embodiment 3, comparative example 3, comparative example 4 processing bacterium bag miscellaneous bacteria infection rate it is minimum, secondly for comparative example 2 handle, finally for The processing of comparative example 1, this illustrates Flos Lonicerae extractive solution with miscellaneous bacteria infection rate during significant decrease Pleurotus eryngii substituting stuff cultivation Function and effect, leopard cat tail beans fruit extracting solution have the synergistic effect for further decreasing miscellaneous bacteria infection rate；CK compares (example 3), right Ratio 1, the mushroom flower bud growth conditions of the processing of comparative example 2 are powerful, and the mushroom flower bud growth conditions of comparative example 3, comparative example 4 processing are not It is very powerful, illustrate that shepherd's purse extracting solution and pea extracting solution have the beneficial effect for promoting the growth of Pleurotus eryngii mushroom flower bud, but the two list Only use cannot promote Pleurotus eryngii mushroom flower bud to grow, and the two need to be with the use of just with said effect；And the processing of comparative example 5 does not have Addition honeysuckle, leopard cat tail beans fruit extracting solution that antibacterial ability is handled with section wood is similar, and during comparative example 6 handles It does not add shepherd's purse extracting solution and pea extracting solution makes mushroom flower bud growth conditions faint, extend relevant fruiting time, reduce apricot The yield of abalone mushroom, in the season of adaptation, fruiting has seriously affected commercially available price not in time；In conclusion the culture medium of the application Bacterium bag infection rate can be greatly reduced, and does not influence the production of Pleurotus eryngii；Calcium in terms of from embodiment 2 and comparative example, Zn-ef ficiency Content compares, rich in the calcium ion and zinc for having a large amount of easy absorption in addition extracting solution shepherd's purse extracting solution, pea extracting solution Ion is more easier angiocarp and is absorbed and utilized, to improve the quality of Pleurotus eryngii.

Test three:

The technical effect of used adhesive and its each component to illustrate the invention is control with embodiment 3,

Control group 1: adhesive is substantially the same manner as Example 3, and difference is to be not added with club fungi extracting solution, the extraction of red Phallus Liquid, poly-aspartate, tea polyphenols, maltodextrin.

Control group 2: adhesive is substantially the same manner as Example 3, and difference is to be not added with club fungi extracting solution.

Control group 3: adhesive is substantially the same manner as Example 3, and difference is to be not added with red Phallus extracting solution.

Control group 4: adhesive is substantially the same manner as Example 3, and difference is to be not added with poly-aspartate.

Control group 5: adhesive is substantially the same manner as Example 3, and difference is to be not added with tea polyphenols.

Control group 6: adhesive is substantially the same manner as Example 3, and difference is to be not added with maltodextrin.

Every mycelia block for taking bacterium bag junction for 1 hour after bag operates is spelled, microscopically observation hyphal cell merges situation, and The content of the Polysaccharide in Pleurotus eryngii of Pleurotus eryngii product, crude protein and each nutritional ingredient is measured, specific data are as follows:

Adhesive uses Hyphal anastomosis effect test tables of data in 3 Pleurotus eryngii substituting stuff cultivation of table

1h

2h

3h

4h

5h

6h

7h

8h

9h

10h

11h

12h

Embodiment 3

-

+

The processing of control group 1

-

The processing of control group 2

-

The processing of control group 3

-

The processing of control group 4

-

+

The processing of control group 5

-

+

The processing of control group 6

-

+

13h

14h

15h

16h

17h

18h

19h

20h

21h

22h

23h

24h

Embodiment 3

+

The processing of control group 1

-

+

The processing of control group 2

-

+

The processing of control group 3

-

+

The processing of control group 4

+

The processing of control group 5

+

The processing of control group 6

+

Note: "-" does not merge, and "+" has merged

Binder solution used in the present invention mainly contains club fungi extracting solution, red Phallus extracting solution, malt paste The Multiple components such as essence, poly-aspartate, tea polyphenols, club fungi extracting solution can promote the growth of hyphal cell wall, promote bacterium indirectly The fusion of silk cell, but have inhibiting effect to the synthesis of Polysaccharide in Pleurotus eryngii, Pleurotus eryngii acid, there is red Phallus extracting solution sterilization to make With, varied bacteria growing between bacterium bag can be inhibited, be conducive to hyphal cell fusion, but between mycelium nutriment transport have obstruction Effect, inventor has found that addition poly-aspartate, tea polyphenols, can promote the conjunction of Polysaccharide in Pleurotus eryngii, Pleurotus eryngii acid At, and add maltodextrin, poly-aspartate can be conducive between mycelium nutriment transport.It is prepared using said components Binder solution, hyphal cell time of fusion can be made to foreshorten to 5 hours, examination of the time of fusion than unused binder solution Processing is tested to shorten more than 4 times.As shown in Table 5, the cell fusion that embodiment 3 and control group 5 are handled is fastest, bacterium bag junction Mycelia block there have been cell fusions in 5 hours；Followed by control group 4 and control group 6 are handled, and are occurred in 12 hours Cell fusion；It is that control group 3 and control group 2 are handled again, cell fusion occurs in 20 hours, 19 hours respectively；Most After be that control group 1 is handled, just occur cell fusion in 23 hours, the cell fusion bacterium of the processing of embodiment 3 compares control group 1 shortens more than 4 times.Above data explanation, in club fungi extracting solution, red Phallus extracting solution, poly-aspartate, tea polyphenols, malt In 5 kinds of ingredients of dextrin, the sequence of cell fusion ability power is promoted to be followed successively by, the red Phallus extracting solution > of club fungi extracting solution > Poly-aspartate=maltodextrin, and tea polyphenols do not embody the ability with cell fusion；Due to different cultivation matrixes at It is different for dividing from the speed of the bonding fusion of adhesive, and in test, we use similar bonding to a variety of cultivation matrixes The speed of agent, discovery bonding fusion is different, and specific principle is the research emphasis of my company of later period.

The Pleurotus eryngii of cultivation harvest by the application carries out random 10 mushrooms and carries out Polysaccharide in Pleurotus eryngii (GB/T15673- 2009), the content (GB/T15673-2009) of crude protein, crude fibre are using the detection of acid detergent method, crude fat using fat Analyzer method is detected, and testing result is as follows:

Table 4

	Polysaccharide (%)	Crude protein (%)	Crude fat	Crude fibre
					Embodiment 3	8.65±0.12	28.21±0.11	2.05±0.12	35.21±0.12
The processing of control group 1	7.21±0.11	17.27±0.12	1.57±0.11	25.36±0.15
					The processing of control group 2	7.84±0.12	19.68±0.10	1.69±0.11	27.67±0.13
The processing of control group 3	7.97±0.15	19.98±0.11	1.62±0.10	29.01±0.13
					The processing of control group 4	8.02±0.13	23.28±0.07	1.85±0.14	31.05±0.14
The processing of control group 5	8.27±0.13	25.17±0.09	1.96±0.11	33.04±0.15
					The processing of control group 6	8.04±0.14	23.89±0.08	1.89±0.10	31.81±0.10

As shown in Table 4, Polysaccharide in Pleurotus eryngii content can be influenced, the power of crude protein component content is ordered as club fungi extracting solution The red Phallus extracting solution > poly-aspartate ≈ maltodextrin > tea polyphenols of >.

Test four:

For the technical effect for further illustrating cultivation of the invention, by taking embodiment 1,2,3,4 as an example, using it is existing on the market Cultivation matrix (CK) carry out planting almond abalone mushroom, randomly select 5 pieces of Pleurotus eryngiis after the temperature of cultivation, illumination, picking and distinguished The averagely single mushroom fresh weight of the average single mushroom of measurement, stem diameter, specific as follows:

Table 5

From the above-mentioned data of table 5 it is found that cultivate the average single mushroom handle of the Pleurotus eryngii that obtains using technical solution of the present invention straight Diameter reaches 32g-40g in 6.2 cm-8.8cm, average single mushroom fresh weight, has reached expected purpose.

The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously Limitation of the scope of the invention therefore cannot be interpreted as.It should be pointed out that coming for those of ordinary skill in the art It says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to guarantor of the invention Protect range.Therefore, protection scope of the present invention should be determined by the appended claims.

Claims

1. a kind of cultural method for improving Pleurotus eryngii quality, it is characterised in that: specifically includes the following steps:

The first step, the preparation of culture bag:

By weight, 20-30 parts of grouts are weighed, 10-20 parts of straw, 60-80 parts of grape stalk, 10-20 parts of litchi meat, egg 5-10 parts of shell, heap fermentation is built after crushing, the central temperature of the 1-3 days control fermentation materials of fermentation is 40-50 DEG C, is controlled within the 4-8 days The central temperature of fermentation material processed is 60-70 DEG C, and the central temperature of the 9-10 days control fermentation materials is 60-80 DEG C, obtains fermentation material After add 15-25 parts of Flos Lonicerae extractive solution, 15-20 parts of beans fruit extracting solution of leopard cat tail, 10-20 parts of shepherd's purse extracting solution, pea mentions 15-20 parts of liquid are taken, 15-25 parts of water, culture bag is packed into after stirring, is placed in autoclave and sterilizes, it is rear naturally cold But to room temperature；Second step, inoculation, bacterium germination:

Third step takes off bag, spells bag, earthing:

Plastic film in culture bag is cut, is sloughed, is placed on the cultivation bed put in order in advance, the culture bag heap lines up length The length of cube, the cuboid connects the length formed by 3-5 culture bag from beginning to end, and the width of the cuboid is by 5-8 The length that culture bag successively forms side by side along the long side direction, the height of the cuboid be by 3-5 culture bag successively from top to bottom The tired heap and tired heap face horizontal accumulation along length cross section, the cuboid it is wide in two side by side culture bag border on gap Matching is provided with ventilation duct, and ventilation duct is arranged along the length direction of cuboid, and the length of the ventilation duct is greater than cuboid Length, extend the mouth two-port of ventilation duct outside cuboid；The mutual consolidation splicing of culture bag, it is molten that every interlayer sprays adhesive Liquid, top layer cover the soil of 2-3cm, and sprinkle water and keep soil wet；

4th step removes flower bud:

When growing more Pleurotus eryngii mushroom flower bud wait cultivate bed, selects and retain healthy and strong mushroom flower bud, and retain two healthy and strong adjacent mushroom flower buds Spacing is 10 centimetres, extracts remaining mushroom flower bud, carries out within every 2 days once removing flower bud, until no longer long fruiting flower bud；

5th step, management of producing mushroom:

Mushroom flower bud grows up, and Pleurotus eryngii fructification length is sprayed on Pleurotus eryngii mushroom handle after 3-5cm and increase element once, often for every 2-3 days It is secondary to spray 2-3ml/ only, it is long until after 10-15cm high until Pleurotus eryngii fructification length；Pleurotus eryngii fructification length is in 8- 13cm, mushroom cover the harvesting in 4-6cm

The temperature, humidity and intensity of illumination of mushroom shed are controlled out, by the way of water spray, ventilation, adjusting illumination in favor of Pleurotus eryngii Growth；

6th step, harvesting:

When Pleurotus eryngii fructification length it is long to 10-15cm after, be Harvesting Date when open and flat, spore not yet launch by mushroom lid, It is harvested in time when fructification bacteria cover diameter reaches 4-6cm；Culture bag continuation moisturizing, and same management of producing mushroom are given after harvesting first time Processing means it is identical, until two damp mushrooms can be harvested.

2. a kind of cultural method for improving Pleurotus eryngii quality according to claim 1, it is characterised in that: the honeysuckle is extracted Liquid is prepared by following methods: by weight, will be honeysuckle 10-20 parts dry, 70-80 DEG C of boiled water 20- is added 30 parts immersion 1-5 minutes, repeat impregnate 3-5 time, by the filtrate filtered every time merge carry out heat be concentrated into the 1.15 of original volume Times after, use power for the supersonic oscillations 10-30S of 300-500W after, stand filter to take supernatant, supernatant is passed through into quality Score is that the ethanol solution of 75-80% is eluted, and collects eluent, obtains the Flos Lonicerae extractive solution of high-purity tea polypenols；

The leopard cat tail beans fruit extracting solution is prepared by following methods: by weight, by fresh 1-5 parts of leopard cat tail beans fruit It 10-30 part of water is added carries out heating and boil to rotting, filter to take filtrate, being subsequently added into pH is that 1.0-2.0 equilibrium liquid is adjusted and filtered Liquid is acid filtrate, then adsorbs acid filtrate by being mounted with the ion column of cation exchange resin, then uses Mass fraction is the ethanol solution elution ionic column of 80-85%, collects eluent, obtains leopard cat tail beans fruit extracting solution.

3. a kind of cultural method for improving Pleurotus eryngii quality according to claim 1, it is characterised in that: the shepherd's purse extracting solution It is to be prepared by following methods: by weight, by fresh shepherd's purse 5-10 parts, 10-20 parts of water stirring is added after cleaning At juice, active carbon is subsequently added into except depigmentaton, is subsequently added into the ethanol solution that mass fraction is 80-85% and is extracted 10-15 hours, After evaporating ethanol solution, leaching liquor is poured into the ion exchange column for being mounted with macroporous absorbent resin with flow velocity for 1-2ml/s It is adsorbed on inner wall, is then eluted again with the ethanol solution that mass fraction is 75-80%, the eluent evaporation after elution Ethanol solution is removed, shepherd's purse extracting solution can be obtained；

The pea extracting solution is prepared by following methods: by weight, fresh 5-10 parts of pea benevolence being added It stirs and is slurried after 5-10 parts of water, being subsequently added into 20-50 parts of concentration is alcohol solution dipping 3-8 hours of 75-85%, is then returned Stream extracts, and extracts 3-5 times, extracts 0.2-0.3 hours every time, merges the filtrate extracted every time, obtains pea extracting solution.

4. a kind of cultural method for improving Pleurotus eryngii quality according to claim 1, it is characterised in that: described adhesive solution It is to be prepared by following methods: by weight, by 10-20 parts of club fungi extracting solution, 8-15 parts of red Phallus extracting solution, wheat 5-8 parts of bud dextrin, 3-8 parts of poly-aspartate, 0.2-0.5 parts of tea polyphenols, 3-8 parts of sucrose, 0.2-0.5 parts of vitamin B1, core yellow It is 0.2-0.5 parts plain, 0.1-0.2 parts of potassium fulvate, 0.2-0.5 parts of ammonium metaphosphate, 0.2-0.5 parts of EDTA- iron, EDTA- zinc 0.2- It 0.4 part, 0.1-0.2 parts of EDTA- calcium, 0.2-0.3 parts of EDTA- magnesium, 50-60 parts of water, stirs evenly obtained；

The club fungi extracting solution is prepared by following methods: by weight, by fresh club fungi 10-15 parts, water 100-200 parts, it is broken into homogenate after mixing, is extracted 0.2-0.5 hours in 38-40 DEG C of water-bath, auxiliary power during extraction For the ultrasonic wave of 100-200W, then layer filtered through gauze obtains club fungi extracting solution repeatedly；

The red Phallus extracting solution is prepared by following methods: by weight, will be scarlet Phallus 10-20 parts new, water Juice is stirred into after 50-100 parts of mixing, is extracted 0.3-0.6 hours in 38-40 DEG C of water-bath, auxiliary power is 100- during extraction The ultrasonic wave of 200W then obtains red Phallus extracting solution using three layers of filtered through gauze.

5. according to claim 1 it is a kind of improve Pleurotus eryngii quality cultural method, it is characterised in that: the increase element be by It prepares as following formula: by weight, by 0.2-0.5 parts of 2,4-DNP sodium, 0.1-0.3 parts of benzimidazole, amine fresh fat It 0.5-1 parts, 0.1-0.3 parts of zeatin, is dissolved in 3-5 parts of alcohol, adds 0.5-1 parts of Tween-80s, 2000-3000 parts of water, It stirs evenly obtained.

6. according to claim 1 it is a kind of improve Pleurotus eryngii quality cultural method, it is characterised in that: the ventilation duct it is straight Diameter is 1.0cm, and multiple ventholes are offered on tube wall.

7. a kind of cultural method for improving Pleurotus eryngii quality according to claim 1, it is characterised in that: the Pleurotus eryngii choosing Select middle-late ripening variety.

8. a kind of cultural method for improving Pleurotus eryngii quality according to claim 1, it is characterised in that: the culture bag is It is cylindrical；The specification of culture bag: long 40-50cm, directly through 8-10cm.

9. a kind of cultural method for improving Pleurotus eryngii quality according to claim 1, it is characterised in that: the temperature of the mushroom shed out Degree control is in 12-15 DEG C, humid control in 80-90% and intensity of illumination control in the lux 600-700.