CN101781211A - Method for extracting chlorogenic acid from sunflower meal - Google Patents
Method for extracting chlorogenic acid from sunflower meal Download PDFInfo
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- CN101781211A CN101781211A CN200910010178A CN200910010178A CN101781211A CN 101781211 A CN101781211 A CN 101781211A CN 200910010178 A CN200910010178 A CN 200910010178A CN 200910010178 A CN200910010178 A CN 200910010178A CN 101781211 A CN101781211 A CN 101781211A
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- chlorogenic acid
- sunflower meal
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- meal
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Abstract
The invention relates to a method for extracting chlorogenic acid from sunflower meal. The method comprises the following steps: pulverizing sunflower meal, and adding a 1% cellulase solution and a 5% protease solution, wherein the ratio of the cellulase solution to the protease solution is 4:1, the ratio of the sunflower meal to the two enzyme solutions is 1:8, the pH value is regulated to 4, the enzymolysis time is 1.2 hours, and the enzymolysis temperature is 49 DEG C; leaching by using a 45-55% ethanol solution of which the pH value is 3-6 so that chlorogenic acid in the sunflower meal is sufficiently dissolved in the leaching solution, and cooling to the room temperature; and carrying out conventional treatment by using a macroporous resin method, adsorbing, eluting, drying and the like to obtain the chlorogenic acid. The method has the advantages of simple operation, mild reaction conditions and no pollution of the extracted chlorogenic acid, and is suitable for industrialized production; and under certain technological conditions, the yield of chlorogenic acid can reach 1.91%.
Description
Technical field the present invention relates to chlorogenic acid extracting method in a kind of sunflower meal.
The depside that the technical background chlorogenic acid is made up of coffic acid and quinic acid, chemical name 3-O-coffee phthalein quinic acid, molecular formula: C16H18O9, molecular weight: 354.30, be a kind of phenylpropyl alcohol chlorins compound that plant materials produces through shikimic acid pathway in the aerobic repiration process.Extensively in existence and the high dicotyledons, mainly be present in Caprifoliaceae Lonicera, the composite family punt-pole platymiscium, wherein the higher plant of content has: the bark of eucommia, Japanese Honeysuckle, Sunflower Receptacle, the wood that continues, coffee, cocoa tree etc.
Sunflower Receptacle is the higher plant of chlorogenic acid content, and chlorogenic acid content is 1.5%～3.3% in the sunflower seeds according to surveying and determination, and it mainly is distributed in the aleurone layer of certain herbaceous plants with big flowers benevolence or in the protein particulate in the cell.By product when sunflower meal is sunflower seeds system oil generally contains chlorogenic acid 1.1%～4.5%, protein 29%～43%.
More about method and patented technology that chlorogenic acid extracts, but see more from plants such as the bark of eucommia, Japanese Honeysuckle and extract, the rarely found report that from sunflower meal, extracts.As Shandong University's patent (patent No.: 03111946) disclose a kind of method for preparing chlorogenic acid, its method comprises with solution the raw material refluxing extraction, suction filtration, merging filtrate, filtrate decompression concentrates and the crude extract separation and purification, raw materials used is dried Herba Arctii leaf.Concrete technological process is the dried Herba Arctii leaf 3hr of organic solvent gradation refluxing extraction with pH2～3; Extract the back suction filtration at every turn, merging filtrate, filtrate decompression concentrates, and reclaims ethanol, gets the Herba Arctii leaf crude extract; Then, the Herba Arctii leaf crude extract is crossed polyamide column, water and methanol-eluted fractions, elutriant gets the pure product of chlorogenic acid through concentrating, making with extra care.For another example the patent disclosure of Guangzhou Han Fang Modern Chinese Traditional Medicine Research Development Co., Ltd a kind of (patent No.: 200610123973.X) prepare the method for high-purity chlorogenic acid and total flavones from Folium Eucommiae, be specifically related to from Folium Eucommiae, prepare the method for high-purity chlorogenic acid and total flavones.It is that Folium Eucommiae is pulverized, and is 3～6 45%～55% ethanolic soln extraction with the pH value; Get on the extracting solution semipolar JD-1 type macroporous resin column and carry out saturated absorption, water, 20%～45% ethanolic soln, 60%～75% ethanolic soln carry out wash-out successively, respectively chlorogenic acid crude product and contain the dry powder of total flavones; Get silicagel column on the chlorogenic acid crude product, carry out wash-out with ethyl acetate or acetone or methyl alcohol or ethanol, Fractional Collections elutriant, concentrating under reduced pressure, enriched material be with acetone or water recrystallization, the chlorogenic acid elaboration.Last as Jinzhou City, Liaoning Province connect dagger-axe patent disclosure (patent No.: 200510047693.0) a kind of from sunflower seed cake the method for chlorogenic acid extracting and sunflower protein, concrete extracting method is: soak and the stirring sunflower seed cake with common salt aqueous solution; With colloidal mill sunflower seed cake is pulverized, albumen and chlorogenic acid in the sunflower seed cake fully are dissolved in the common salt aqueous solution, and are cooled to room temperature; Then, carry out solid-liquid separation, isolate the aqueous solution that contains albumen and chlorogenic acid, make it pass through macroporous resin column, the chlorogenic acid in the adsorption aqueous solution; Feed liquid after the absorption adds hydrochloric acid, makes albumen separate out precipitation, more above-mentioned feed liquid is carried out solid-liquid separation, isolates the sunflower protein curdled milk; Thin up, the flash distillation sterilization, spraying drying promptly obtains the high purity sunflower protein; Elutriant with methyl alcohol, acetone, ethanol and softening water preparation passes through macroporous resin column, and wash-out is adsorbed on the chlorogenic acid in the macroporous resin column; Evaporation of eluate is evaporated organic solvent fully, obtains the chlorogenic acid aqueous solution; With chlorogenic acid aqueous solution spraying drying, promptly obtain chlorogenic acid dry powder.
The purpose of invention the purpose of this invention is to provide chlorogenic acid extracting method in a kind of new sunflower meal, make in the sunflower meal chlorogenic acid extracting method more advanced and mature, simple and practical, extraction yield is higher, be more suitable for carrying out suitability for industrialized production, and can not cause environmental pollution aborning.
Content the present invention of invention takes following technological method for processing: sunflower meal is pulverized, add concentration and be 1% cellulase and concentration and be 5% proteolytic enzyme, cellulase and proteolytic enzyme enzyme liquor ratio are 4: 1, sunflower meal and two kinds of enzyme liquor ratios are 1: 8, adjust pH is 4, enzymolysis time 1.2 hours, 49 ℃ of hydrolysis temperatures; Be 3～6 45%～55% ethanolic soln lixiviate again with the pH value, the chlorogenic acid in the sunflower meal fully be dissolved in the vat liquor, and be cooled to room temperature; Then, carry out solid-liquid separation, make vat liquor pass through macroporous resin column, the chlorogenic acid in the adsorbent solution; Elutriant with methyl alcohol equal solvent and softening water preparation passes through macroporous resin column, and wash-out is adsorbed on the chlorogenic acid in the macroporous resin column; Evaporation of eluate is evaporated organic solvent fully, obtains the chlorogenic acid aqueous solution; With chlorogenic acid aqueous solution spraying drying, promptly obtain chlorogenic acid dry powder.
The positively effect of invention adopts the aforesaid method chlorogenic acid extracting, operate comparatively simple, reaction conditions gentleness, and the chlorogenic acid of extraction is pollution-free, is more suitable for suitability for industrialized production, under certain process conditions, the chlorogenic acid yield can reach 1.91%.
Embodiment takes by weighing 500 gram sunflower meals, carries out pulverization process, adds concentration and be 1% cellulase 600ml and concentration and be 5% proteolytic enzyme 150ml, and sunflower meal and enzyme liquor ratio are 1: 8, and adjust pH is 4, enzymolysis time 1.2 hours, 49 ℃ of hydrolysis temperatures; Be 3～6 45%～55% ethanolic soln lixiviate again with the pH value, the chlorogenic acid in the sunflower meal fully be dissolved in the vat liquor, and be cooled to room temperature; Then, carry out solid-liquid separation, make vat liquor pass through macroporous resin column, the chlorogenic acid in the adsorbent solution; Elutriant with methyl alcohol equal solvent and softening water preparation passes through macroporous resin column, and wash-out is adsorbed on the chlorogenic acid in the macroporous resin column; Evaporation of eluate is evaporated organic solvent fully, obtains the chlorogenic acid aqueous solution; With chlorogenic acid aqueous solution spraying drying, promptly obtain chlorogenic acid dry powder.
Claims (1)
1. chlorogenic acid extracting method in the sunflower meal, it is characterized in that technological method for processing is after sunflower meal is pulverized, add concentration and be 1% cellulase and concentration and be 5% proteolytic enzyme, cellulase and proteolytic enzyme enzyme liquor ratio are 4: 1, sunflower meal and two kinds of enzyme liquor ratios are 1: 8, adjust pH is 4, enzymolysis time 1.2 hours, 49 ℃ of hydrolysis temperatures.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910010178A CN101781211A (en) | 2009-01-18 | 2009-01-18 | Method for extracting chlorogenic acid from sunflower meal |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910010178A CN101781211A (en) | 2009-01-18 | 2009-01-18 | Method for extracting chlorogenic acid from sunflower meal |
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| CN101781211A true CN101781211A (en) | 2010-07-21 |
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| CN200910010178A Pending CN101781211A (en) | 2009-01-18 | 2009-01-18 | Method for extracting chlorogenic acid from sunflower meal |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103937614A (en) * | 2013-12-13 | 2014-07-23 | 广西科技大学 | Leaching liquor for extracting grease from sunflower seeds oil meal |
| CN106916021A (en) * | 2017-04-12 | 2017-07-04 | 山东佐田氏生物科技有限公司 | A kind of double source humic acid biological fertilizer and preparation method and application |
-
2009
- 2009-01-18 CN CN200910010178A patent/CN101781211A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103937614A (en) * | 2013-12-13 | 2014-07-23 | 广西科技大学 | Leaching liquor for extracting grease from sunflower seeds oil meal |
| CN103937614B (en) * | 2013-12-13 | 2016-06-08 | 广西科技大学 | A kind of vat liquor extracting grease from the sunflower seed oil dregs of rice |
| CN106916021A (en) * | 2017-04-12 | 2017-07-04 | 山东佐田氏生物科技有限公司 | A kind of double source humic acid biological fertilizer and preparation method and application |
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Application publication date: 20100721 |