CN115362924A - Method for promoting aquatic plant to take root in gravel environment - Google Patents
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Abstract
The invention provides a method for promoting aquatic plants to root in a gravel environment, which comprises the following steps: s1, mixing an enterobacter cloacae fermentation liquor and a bacillus methylotrophicus fermentation liquor according to a volume ratio of 1: 1-3, mixing uniformly to obtain a mixed fermentation liquor; s2, preparing a regeneration culture medium; the regeneration culture medium is prepared by mixing an MS culture medium, a rice vinegar solution, molasses alcohol fermentation liquor, potassium fulvate and mixed fermentation liquor; and S3, diluting the regeneration culture medium of the S2 with sterile water, transferring the diluted regeneration culture medium into a culture container paved with a gravel layer, inserting the asexual propagules of the aquatic plants into a plurality of floating blocks, and then placing the floating blocks into the culture container for culture. The method utilizes the mixed fermentation liquor prepared from the enterobacter cloacae fermentation liquor and the bacillus methylotrophicus fermentation liquor, and is matched with other components to promote the growth of the root system of the aquatic plant, so that the rooting rate of the aquatic plant can be accelerated, the growth cycle of the root system can be shortened, and the survival rate of the aquatic plant can be improved.
Description
Technical Field
The invention relates to the technical field of plant cultivation, in particular to a method for promoting aquatic plants to take root in a gravel environment.
Background
Aquatic plants are plants that grow in water or marshes, such as lotus, calamus, cattail, reed, juncus, water lily, gordon euryale, duckweed, and the like. The aquatic plants not only have good viewing function, but also can play a role in purifying water quality, for example, absorbing or enriching harmful substances such as heavy metals and the like, maintaining variety of species and the like.
The purification effect of aquatic plants on water quality is often related to root systems of the aquatic plants. Compared with terrestrial plants, part of the aquatic plants live in water, do not need to absorb water and nutrients through developed root systems, and can not reduce the loss of water, so that the root epidermis of the aquatic plants is thin, the root systems are not developed, and the root systems of the aquatic plants mainly play a role in fixing the plants. And the roots of the aquatic plants are developed, so that a dense filter layer can be formed in the water body, and the adsorption or interception effect on the water body is achieved. The aquatic plants can also increase the content of dissolved oxygen in the water body through the root system, and provide or improve survival conditions for other species. Therefore, by restoring the aquatic plant system, the variety of enclosure species is facilitated, and the structure of the aquatic ecosystem is more stable.
However, in the process of cultivating aquatic plants, it is found that the growth period of the root systems of the aquatic plants is relatively long, and root rot, withering and death and the like often occur in the cultivation process. Therefore, a method for promoting the rooting of aquatic plants, which shortens the growth cycle of root systems and improves the survival rate, is urgently needed.
Molasses alcohol fermentation liquor, which is a by-product of sugar production from sugarcane, namely molasses, is fermented by adding alcohol yeast, and a stock solution left after alcohol is extracted by evaporation; the stock solution generally contains 70-80% of active water-soluble organic matters, and researches show that the molasses alcohol fermentation liquor contains biogenic NPK 6%, 40-48% of humic acid, 12-18% of amino acid and 20% of crude protein, and is also rich in chelated medium and trace elements such as calcium, sulfur, silicon, magnesium, zinc, copper and the like. In the fertilizer using molasses alcohol fermentation liquor as the main raw material, organic nutrition of soil can be supplemented, root activity is improved, and disease resistance and stress resistance of crops can be improved.
The cultivation of the asexual propagules of the aquatic plants can normally root under proper culture conditions and complete normal growth and development, but the report of using molasses alcohol fermentation liquor for rooting cultivation of the aquatic plants is not seen in the prior art.
Disclosure of Invention
In order to solve the technical problems, the invention aims to provide a method for promoting aquatic plants to root in a gravel environment.
In order to achieve the above object, the technical solution of the present invention is as follows.
A method for promoting aquatic plant rooting in a gravel environment, comprising the steps of:
s1, mixing an enterobacter cloacae fermentation liquor and a bacillus methylotrophicus fermentation liquor according to a volume ratio of 1: 1-3, uniformly mixing to obtain mixed fermentation liquor;
s2, preparing a regeneration culture medium; the regeneration culture medium is prepared from an MS culture medium, a rice vinegar solution, molasses alcohol fermentation liquor, potassium fulvate and mixed fermentation liquor according to a mass ratio of 1:0.02 to 0.1:0.1 to 0.3:0.02 to 0.1:0.2 to 0.8 percent;
s3, diluting the regeneration culture medium of the S2 by 50-100 times by using sterile water to obtain a culture solution; transferring the culture solution into a culture container paved with a gravel layer, inserting the asexual propagules of the aquatic plants into a plurality of floating blocks, and then placing the floating blocks into the culture container for culture.
Further, in S1, the enterobacter cloacae fermentation broth is prepared by the following method:
adding tryptophan and molasses into an LB culture medium, then inoculating enterobacter cloacae, carrying out shake culture at 25-30 ℃ for 24-48 h, centrifuging, and taking supernatant to obtain enterobacter cloacae fermentation liquor.
Furthermore, 0.5 to 1g of tryptophan and 30 to 50g of molasses are added to 1L of LB medium.
Further, the conditions of centrifugation were as follows: centrifuging at 10000r/min for 10-30 min at normal temperature.
Furthermore, in S1, the effective viable count of the Enterobacter cloacae fermentation liquor is more than or equal to 4 multiplied by 10 8 CFU/ml。
Further, in S1, the Bacillus methylotrophicus fermentation broth is prepared by the following method:
adding rapeseed cake meal into an LB culture medium, then inoculating bacillus methylotrophicus, and fermenting and culturing for 4-7 days at 25-30 ℃ to obtain bacillus methylotrophicus fermentation liquor; 1-10 g of rapeseed cake is added in each 1L of LB culture medium.
Furthermore, in S1, the number of effective viable bacteria contained in the Bacillus methylotrophicus fermentation liquid is more than or equal to 8 multiplied by 10 8 CFU/ml。
Further, in S2, the water content of the rice vinegar solution is 70-90%; the water content of the molasses alcohol fermentation liquor is 50-75%.
Further, in S3, the vegetative propagules of the aquatic plants are tubers or branches with tender leaves or buds.
Further, in S3, the depth of the asexual propagules of the aquatic plants inserted into the culture solution is 0.8-1.2 cm.
Further, the aquatic plant is any one of calamus, cattail and reed.
The invention has the beneficial effects that:
1. the mixed fermentation liquor prepared by the enterobacter cloacae fermentation liquor and the methylotrophic bacillus fermentation liquor is mixed with the MS culture medium, the rice vinegar solution, the molasses alcohol fermentation liquor and the potassium fulvate to prepare the culture liquor, so that the growth of the root system of the aquatic plant is promoted, the rooting rate of the aquatic plant can be increased, the growth cycle of the root system is shortened, and the survival rate of the aquatic plant can be increased.
2. In the invention, because the fermentation liquor of the enterobacter cloacae contains the growth hormone IAA, the increase of the content of the growth hormone IAA in the fermentation liquor can be further promoted by adding a small amount of tryptophan in an LB culture medium, and the growth hormone IAA is matched with other components to accelerate the rooting rate.
3. In the invention, the bacillus methylotrophicus fermentation liquor contains nutrient substances required by the growth of aquatic plants, and the nutrient substances can also promote the growth of root systems; the methylotrophic bacillus fermentation liquor can also promote the growth of other water culture growth-promoting bacteria in a water culture environment, and is beneficial to the conversion of water culture nutrient substances while increasing the diversity of microorganisms, so that the survival rate of aquatic plants is further improved.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Bacillus methylotrophicus (LW-6, not less than 8 x 10) 8 Seeds/g), shanxi Hengtian bio-agriculture, ltd.
Enterobacter cloacae (Enterobacter cloacae subsp. Cloacae, E26-1, 4 × 10 or more 8 CFU/mL), shanghai Xuan Yah Biotech, inc.
Preparation of LB medium (liquid medium): 10g/L of tryptone, 5g/L of yeast extract and 10g/L of sodium chloride; pH7.4.
The experimental methods described in the following examples are all conventional methods unless otherwise specified; the reagents and materials are commercially available, unless otherwise specified.
Example 1
A method for promoting aquatic plant rooting in a gravel environment, comprising the steps of:
s1, adding 0.8g of tryptophan and 40g of molasses into each 1L of LB culture medium, and mixing to obtain a culture solution; then inoculating enterobacter cloacae, culturing for 48h at 30 ℃ in a shaking table, centrifuging for 20min at 10000r/min at normal temperature, and taking supernatant to obtain enterobacter cloacae fermentation liquor; the effective viable count of the fermentation liquor of the enterobacter cloacae is more than or equal to 4 multiplied by 10 8 CFU/ml。
S2, adding 8g of rapeseed cakes into each 1L of LB culture medium, and mixing to obtain a culture solution; then inoculating bacillus methylotrophicus, and fermenting and culturing for 6d at 30 ℃ to obtain bacillus methylotrophicus fermentation liquor; the number of effective viable bacteria contained in the bacillus methylotrophicus fermentation liquor is more than or equal to 8 multiplied by 10 8 CFU/ml。
S3, mixing the enterobacter cloacae fermentation liquor and the bacillus methylotrophicus fermentation liquor according to the volume ratio of 1:2, uniformly mixing to obtain mixed fermentation liquor.
S4, preparing a regeneration culture medium; the regeneration culture medium is prepared from an MS culture medium, a rice vinegar solution, molasses alcohol fermentation liquor, potassium fulvate and mixed fermentation liquor according to a mass ratio of 1:0.08:0.2:0.08:0.6, mixing;
s5, diluting the regeneration culture medium by 60 times with sterile water to obtain a culture solution; transferring the culture solution into a culture container paved with a gravel layer, inserting the asexual propagules of the aquatic plants into a plurality of floating blocks, wherein the depth of the asexual propagules of the aquatic plants inserted into the culture solution is 1.0cm, and then placing the aquatic plants into the culture container for culture.
Example 2
A method for promoting aquatic plant rooting in a gravel environment, comprising the steps of:
s1, adding 1g of tryptophan and 50g of molasses into 1L of LB culture medium, and mixing to obtain a culture solution; inoculating Enterobacter cloacae, shake culturing at 30 deg.C for 40 hr, centrifuging at room temperature at 10000r/min for 30min, collecting supernatantObtaining an enterobacter cloacae fermentation liquor; the effective viable count of the fermentation liquor of the enterobacter cloacae is more than or equal to 4 multiplied by 10 8 CFU/ml。
S2, adding 10g of rapeseed cakes into each 1L of LB culture medium, and mixing to obtain a culture solution; then inoculating bacillus methylotrophicus, and fermenting and culturing for 7d at 30 ℃ to obtain bacillus methylotrophicus fermentation liquor; the Bacillus methylotrophicus fermentation liquid contains more than or equal to 8 multiplied by 10 effective viable bacteria 8 CFU/ml。
S3, mixing the enterobacter cloacae fermentation liquor and the bacillus methylotrophicus fermentation liquor according to a volume ratio of 1:3, uniformly mixing to obtain the mixed fermentation liquor.
S4, preparing a regeneration culture medium; the regeneration culture medium is prepared from an MS culture medium, a rice vinegar solution, molasses alcohol fermentation liquor, potassium fulvate and mixed fermentation liquor according to a mass ratio of 1:0.02:0.1:0.1:0.2 mixing to obtain;
s5, diluting the regeneration culture medium by 50 times with sterile water to obtain a culture solution; transferring the culture solution into a culture container paved with a gravel layer, inserting the asexual propagules of the aquatic plants into a plurality of floating blocks, wherein the depth of the asexual propagules of the aquatic plants inserted into the culture solution is 1.2cm, and then placing the aquatic plants into the culture container for culture.
Example 3
A method for promoting aquatic plant rooting in a gravel environment, comprising the steps of:
s1, adding 0.5g of tryptophan and 30g of molasses into each 1L of LB culture medium, and mixing to obtain a culture solution; then inoculating enterobacter cloacae, culturing for 24h at 25 ℃ in a shaking table, centrifuging for 10min at 10000r/min at normal temperature, and taking supernatant to obtain enterobacter cloacae fermentation liquor; the effective viable count of the fermentation liquor of the enterobacter cloacae is more than or equal to 4 multiplied by 10 8 CFU/ml。
S2, adding 1g of rapeseed cake meal into each 1L of LB culture medium, and mixing to obtain a culture solution; then inoculating bacillus methylotrophicus, and fermenting and culturing for 4d at 25 ℃ to obtain bacillus methylotrophicus fermentation liquor; the number of effective viable bacteria contained in the bacillus methylotrophicus fermentation liquor is more than or equal to 8 multiplied by 10 8 CFU/ml。
S3, mixing the enterobacter cloacae fermentation liquor and the bacillus methylotrophicus fermentation liquor according to a volume ratio of 1: 1-3, and mixing uniformly to obtain the mixed fermentation liquor.
S4, preparing a regeneration culture medium; the regeneration culture medium is prepared from an MS culture medium, a rice vinegar solution, molasses alcohol fermentation liquor, potassium fulvate and mixed fermentation liquor according to a mass ratio of 1:0.1:0.3:0.02:0.8, mixing;
s5, diluting the regeneration culture medium by 100 times with sterile water to obtain a culture solution; transferring the culture solution into a culture container paved with a gravel layer, inserting the asexual propagules of the aquatic plants into a plurality of floating blocks, wherein the depth of the asexual propagules of the aquatic plants inserted into the culture solution is 0.8cm, and then placing the aquatic plants into the culture container for culture.
Comparative example 1
A method for promoting aquatic plant rooting in a gravel environment, which is different from example 1 in that tryptophan is not added to the preparation of an enterobacter cloacae fermentation broth; the method comprises the following steps:
s1, adding 40g of molasses into each 1L of LB culture medium, and mixing to obtain a culture solution; then inoculating enterobacter cloacae, culturing for 48h at 30 ℃ in a shaking table, centrifuging for 20min at 10000r/min at normal temperature, and taking supernatant to obtain enterobacter cloacae fermentation liquor; the effective viable count of the enterobacter cloacae fermentation liquor is more than or equal to 4 multiplied by 10 8 CFU/ml。
S2, adding 8g of rapeseed cakes into each 1L of LB culture medium, and mixing to obtain a culture solution; then inoculating bacillus methylotrophicus, and fermenting and culturing for 6d at 30 ℃ to obtain bacillus methylotrophicus fermentation liquor; the number of effective viable bacteria contained in the bacillus methylotrophicus fermentation liquor is more than or equal to 8 multiplied by 10 8 CFU/ml。
S3, mixing the enterobacter cloacae fermentation liquor and the bacillus methylotrophicus fermentation liquor according to a volume ratio of 1:2, uniformly mixing to obtain mixed fermentation liquor.
S4, preparing a regeneration culture medium; the regeneration culture medium is prepared from an MS culture medium, a rice vinegar solution, molasses alcohol fermentation liquor, potassium fulvate and mixed fermentation liquor according to a mass ratio of 1:0.08:0.2:0.08:0.6 mixing to obtain;
s5, diluting the regeneration culture medium by 60 times with sterile water to obtain a culture solution; transferring the culture solution into a culture container paved with a gravel layer, inserting the vegetative propagules of the aquatic plants into a plurality of floating blocks, wherein the depth of the vegetative propagules of the aquatic plants inserted into the culture solution is 1.0cm, and then placing the aquatic plants in the culture container for culture.
Comparative example 2
A method for promoting aquatic plant rooting in a gravel environment, which is different from the method in example 1 in that enterobacter cloacae fermentation broth is not added to the mixed fermentation broth; the method comprises the following steps:
s1, adding 8g of rapeseed cake meal into each 1L of LB culture medium, and mixing to obtain a culture solution; then inoculating bacillus methylotrophicus, fermenting and culturing for 6d at 30 ℃ to obtain bacillus methylotrophicus fermentation liquid; the number of effective viable bacteria contained in the bacillus methylotrophicus fermentation liquor is more than or equal to 8 multiplied by 10 8 CFU/ml。
S2, mixing the bacillus methylotrophicus fermentation liquor with water according to the volume ratio of 1:2, uniformly mixing to obtain mixed fermentation liquor.
S3, preparing a regeneration culture medium; the regeneration culture medium is prepared from an MS culture medium, a rice vinegar solution, molasses alcohol fermentation liquor, potassium fulvate and mixed fermentation liquor according to a mass ratio of 1:0.08:0.2:0.08:0.6 mixing to obtain;
s4, diluting the regeneration culture medium by 60 times with sterile water to obtain a culture solution; transferring the culture solution into a culture container paved with a gravel layer, inserting the asexual propagules of the aquatic plants into a plurality of floating blocks, wherein the depth of the asexual propagules of the aquatic plants inserted into the culture solution is 1.0cm, and then placing the aquatic plants into the culture container for culture.
Comparative example 3
A method for promoting aquatic plant rooting in a gravel environment, which is different from the method in example 1 in that a bacillus methylotrophicus fermentation broth is not added to a mixed fermentation broth; the method comprises the following steps:
s1, adding 0.8g of tryptophan and 40g of molasses into each 1L of LB culture medium, and mixing to obtain a culture solution; inoculating Enterobacter cloacae, shake culturing at 30 deg.C for 48 hr, centrifuging at room temperature at 10000r/min for 20min, collecting supernatant to obtain Enterobacter cloacae fermentationLiquid; the effective viable count of the fermentation liquor of the enterobacter cloacae is more than or equal to 4 multiplied by 10 8 CFU/ml。
S2, mixing the Enterobacter cloacae fermentation liquor with water according to the volume ratio of 1:2, uniformly mixing to obtain mixed fermentation liquor.
S3, preparing a regeneration culture medium; the regeneration culture medium is prepared from an MS culture medium, a rice vinegar solution, molasses alcohol fermentation liquor, potassium fulvate and mixed fermentation liquor according to a mass ratio of 1:0.08:0.2:0.08:0.6 mixing to obtain;
s4, diluting the regeneration culture medium by 60 times with sterile water to obtain a culture solution; transferring the culture solution into a culture container paved with a gravel layer, inserting the vegetative propagules of the aquatic plants into a plurality of floating blocks, wherein the depth of the vegetative propagules of the aquatic plants inserted into the culture solution is 1.0cm, and then placing the aquatic plants in the culture container for culture.
Comparative example 4
A method for promoting aquatic plants to take root in a gravel environment is different from the method in the embodiment 1 in that molasses alcohol fermentation liquor is not added into a culture solution; the method comprises the following steps:
s1, adding 0.8g of tryptophan and 40g of molasses into each 1L of LB culture medium, and mixing to obtain a culture solution; then inoculating enterobacter cloacae, culturing for 48h at 30 ℃ in a shaking table, centrifuging for 20min at 10000r/min at normal temperature, and taking supernatant to obtain enterobacter cloacae fermentation liquor; the effective viable count of the fermentation liquor of the enterobacter cloacae is more than or equal to 4 multiplied by 10 8 CFU/ml。
S2, adding 8g of rapeseed cakes into each 1L of LB culture medium, and mixing to obtain a culture solution; then inoculating bacillus methylotrophicus, and fermenting and culturing for 6d at 30 ℃ to obtain bacillus methylotrophicus fermentation liquor; the number of effective viable bacteria contained in the bacillus methylotrophicus fermentation liquor is more than or equal to 8 multiplied by 10 8 CFU/ml。
S3, mixing the enterobacter cloacae fermentation liquor and the bacillus methylotrophicus fermentation liquor according to the volume ratio of 1:2, uniformly mixing to obtain mixed fermentation liquor.
S4, preparing a regeneration culture medium; the regeneration culture medium is prepared from an MS culture medium, a rice vinegar solution, potassium fulvate and a mixed fermentation liquid according to a mass ratio of 1:0.08:0.08:0.6 mixing to obtain;
s5, diluting the regeneration culture medium by 60 times with sterile water to obtain a culture solution; transferring the culture solution into a culture container paved with a gravel layer, inserting the asexual propagules of the aquatic plants into a plurality of floating blocks, wherein the depth of the asexual propagules of the aquatic plants inserted into the culture solution is 1.0cm, and then placing the aquatic plants into the culture container for culture.
Taking calamus as an example, tubers with 2-4 tender shoots of calamus are taken and cultured by the methods of examples 1-3 and comparative examples 1-4.
1. IAA content detection
The IAA content was determined by high performance liquid chromatography based on "assay of indoleacetic acid (IAA) content in DB 51/T2384-2017 plants and seeds (fruits)", and the results are shown in Table 1.
TABLE 1IAA content test results
As can be seen from Table 1, compared with comparative example 1, the addition of tryptophan in examples 1 to 3 can effectively increase the IAA content of the Enterobacter cloacae fermentation broth; compared with the comparative examples 1-2, the addition of the Enterobacter cloacae fermentation broth in the examples 1-3 can effectively improve the endogenous IAA content of the calamus plants. Therefore, the addition of tryptophan can promote the increase of the content of the IAA in the fermentation liquor of the Enterobacter cloacae, the addition of the fermentation liquor of the Enterobacter cloacae can further promote the increase of the content of the endogenous IAA in the Acorus calamus plant, and the accumulation of the exogenous IAA can further promote the accumulation of the endogenous IAA so as to promote the growth of the Acorus calamus plant.
2. Influence of plant root growth
The growth of root system of the calamus tuber cultured by the methods of examples 1-3 and comparative examples 1-4 was examined and shown in table 2.
TABLE 2 root growth
| Rooting percentage/%) | Rooting Rate/d | Root length/cm | |
| Example 1 | 100.0 | 10 | 2.17 |
| Example 2 | 100.0 | 10 | 2.23 |
| Example 3 | 100.0 | 11 | 2.15 |
| Comparative example 2 | 98.7 | 19 | 1.73 |
| Comparative example 3 | 83.1 | 13 | 1.15 |
| Comparative example 4 | 90.2 | 15 | 1.48 |
As can be seen from the results in Table 2, the rooting rate and root length of the plants of examples 1-3 are higher than those of the plant of comparative example 2, which indicates that the Enterobacter cloacae fermentation broth can promote the growth of the root system and shorten the growth cycle. The rooting rate, rooting rate and root length of the plants of examples 1-3 are all obviously higher than those of the plant of comparative example 3, which shows that the bacillus methylotrophicus fermentation liquid can not only promote the growth of the root system, but also effectively improve the survival rate. The rooting rate, rooting rate and root length of the plants in examples 1-3 are all higher than those of the plant in comparative example 4, which shows that the molasses alcohol fermentation liquor can improve the root activity and further improve the rooting survival rate. Therefore, the method of embodiments 1 to 3 of the present invention utilizes the mixed fermentation broth prepared from the enterobacter cloacae fermentation broth and the bacillus methylotrophicus fermentation broth, and mixes the mixed fermentation broth with the MS culture medium, the rice vinegar solution, the molasses alcohol fermentation broth, and the potassium fulvate to prepare the culture solution, which is used for promoting the growth of the root system of the aquatic plant, and can not only accelerate the rooting rate of the aquatic plant, shorten the growth cycle of the root system, but also improve the survival rate of the aquatic plant.
The present invention is not limited to the above preferred embodiments, and any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A method for promoting aquatic plant rooting in a gravel environment, comprising the steps of:
s1, mixing an enterobacter cloacae fermentation liquor and a bacillus methylotrophicus fermentation liquor according to a volume ratio of 1: 1-3, mixing uniformly to obtain a mixed fermentation liquor;
s2, preparing a regeneration culture medium; the regeneration culture medium is prepared from an MS culture medium, a rice vinegar solution, molasses alcohol fermentation liquor, potassium fulvate and mixed fermentation liquor according to a mass ratio of 1: 0.02-0.1: 0.1 to 0.3:0.02 to 0.1:0.2 to 0.8 percent;
s3, diluting the regeneration culture medium of the S2 by 50-100 times with sterile water to obtain a culture solution; transferring the culture solution into a culture container paved with a gravel layer, inserting the asexual propagules of the aquatic plants into a plurality of floating blocks, and then placing the floating blocks into the culture container for culture.
2. The method for promoting aquatic plant rooting in a gravel environment of claim 1, wherein in S1, the enterobacter cloacae fermentation broth is prepared by the following method:
adding tryptophan and molasses into an LB culture medium, then inoculating enterobacter cloacae, carrying out shake culture at 25-30 ℃ for 24-48 h, centrifuging, and taking supernatant to obtain enterobacter cloacae fermentation liquor.
3. The method for promoting aquatic plant rooting in a gravel environment of claim 2, wherein 0.5-1 g of tryptophan and 30-50 g of molasses are added to 1LLB medium.
4. The method of claim 2, wherein the conditions of centrifugation are as follows: centrifuging at 10000r/min for 10-30 min at normal temperature.
5. The method for promoting aquatic plant rooting in a gravel environment according to claim 1 or 2, wherein in S1, the effective viable count of the Enterobacter cloacae fermentation broth is not less than 4 x 10 8 CFU/ml。
6. The method for promoting aquatic plant rooting in a gravel environment of claim 1, wherein in S1, the bacillus methylotrophicus fermentation broth is prepared by the following method:
adding rapeseed cake meal into an LB culture medium, then inoculating bacillus methylotrophicus, and fermenting and culturing for 4-7 days at 25-30 ℃ to obtain bacillus methylotrophicus fermentation liquor; 1-10 g of rapeseed cake meal is added in each 1LLB culture medium.
7. The method for promoting aquatic plant rooting in the gravel environment of claim 1 or 6, wherein S1, the number of effective viable bacteria contained in the Bacillus methylotrophicus fermentation broth is more than or equal to 8 x 10 8 CFU/ml。
8. The method for promoting aquatic plant rooting in a gravel environment of claim 1, wherein in S2, the water content of the rice vinegar solution is 70-90%; the water content of the molasses alcohol fermentation liquor is 50-75%.
9. The method for promoting rooting of aquatic plants in a gravel environment of claim 1, wherein in S3, the vegetative propagules of the aquatic plants are tubers or branches with young leaves or buds.
10. The method for promoting rooting of aquatic plants under the gravel environment of claim 1, wherein, in S3, the vegetative propagules of the aquatic plants are inserted into the culture solution to a depth of 0.8 to 1.2cm.
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