CN115322056A - Application of a water-retaining agent and application of compound biological organic fertilizer with water-retaining function - Google Patents

Abstract

The invention belongs to the technical field of microbial fertilizers, and in particular relates to the application of a compound biological organic fertilizer with a water retention function. The invention provides the application of a composite biological organic fertilizer added with a water-retaining agent in improving the activity of functional bacteria in compacted soil and/or enhancing the fertilizer-retention property of compacted soil. The compound biological organic fertilizer obtained by adding the water-retaining agent in the present invention has the characteristics of convenient use, labor saving and fertilizer enhancement effect. The application of the compound bio-organic fertilizer in the compacted red soil can improve the soil's ability to retain fertilizer and maintain the stability of the functional bacteria in the bio-organic fertilizer after application, thereby improving the problems of easy loss of fertilizer efficiency and low activity of functional microorganisms in the compacted soil. , which is further conducive to promoting crop growth. The experimental results of the examples show that after the compound bio-organic fertilizer is applied for 15 days, the soil is moist and loose; the decline in the number of functional bacteria is slowed;

Description

Application of a water-retaining agent and compound bio-organic fertilizer with water-retaining function

This application is a divisional application with an application date of November 26, 2020, an application number of 202011348868.2, and an invention title of "A Composite Biological Organic Fertilizer with Water-Retaining Function and Its Preparation Method".

technical field

The invention belongs to the technical field of microbial fertilizers, and in particular relates to the application of a compound biological organic fertilizer with water retention function.

Background technique

Bio-organic fertilizer refers to a type of microbial fertilizer and organic fertilizer that is composed of specific functional microorganisms and organic materials that are mainly sourced from animal and plant residues (such as livestock and poultry manure, crop straw, etc.) and have been harmlessly treated and decomposed. Fertilizer with fertilizer effect. After the bio-organic fertilizer is applied to the soil, under good soil conditions, it will breed beneficial bacteria, improve the function of the crop rhizosphere flora, and play a role in promoting crop growth and improving the soil environment. The bacteria used in bio-organic fertilizers generally belong to aerobic, heterotrophic, and mesophilic microorganisms. When the soil is well ventilated, rich in nutrients, suitable for temperature and humidity (temperature 5-35°C, humidity about 60%), and moderate pH (pH5.5- 6.5), it is conducive to the reproduction and growth of microorganisms.

However, in areas with high temperature and drought and soil compaction, the soil is mostly in a state of poor air permeability, lack of nutrient loss, and large temperature difference between day and night. On the one hand, the problem of soil compaction is common, and on the other hand, the dry hills and mountainous areas in the north and south lack water, which is not conducive to the function of the functional bacteria of bio-organic fertilizers. Under the above adverse conditions, the growth of microorganisms will obviously slow down or stagnate, and the fertilizer efficiency of bio-organic fertilizers will be affected. In agricultural production, there is a method of compound application of bio-organic fertilizer and water-retaining agent, that is, applying the two to the soil step by step. Although this method has a certain effect on improving soil fertility, it only has water-retaining ability where the water-retaining agent gathers. The effect of improving the fertilizer efficiency of organic fertilizer functional bacteria is not obvious, and it does not solve the problem of poor soil fertility, and it increases the labor force.

Therefore, it is necessary to provide a compound bio-organic fertilizer with water retention function, which can effectively improve the bio-fertilizer efficiency and fertilizer retention capacity of the bio-organic fertilizer, thereby promoting crop growth.

Contents of the invention

The purpose of the present invention is to provide a water-retaining agent used as a compound bio-organic fertilizer raw material to improve the fertilizer retention capacity and/or bio-fertilizer efficiency of the bio-organic fertilizer. The invention applies the compound bio-organic fertilizer containing the water-retaining agent to hardened red soil, which can enhance the bio-fertilizer effect and fertilizer retention capacity of the bio-organic fertilizer, and promote the growth of crops.

In order to achieve the above object, the present invention provides the following technical solutions:

The invention provides a compound bio-organic fertilizer with water-retaining function, which comprises a bio-organic fertilizer and a water-retaining agent, and the mass ratio of the bio-organic fertilizer and the water-retaining agent is (100-500):1.

Preferably, the mass ratio of the bio-organic fertilizer to the water-retaining agent is (200-300):1.

Preferably, the water retaining agent is a polyacrylate water retaining agent.

Preferably, the water retaining agent includes ammonium polyacrylate and/or potassium polyacrylate.

Preferably, the bio-organic fertilizer includes an organic carrier and functional bacteria; the functional bacteria include Paenibacillus jelly-like and/or Bacillus subtilis.

Preferably, the organic carrier is obtained by fermenting and decomposing waste organic resources under the action of a decomposing agent.

Preferably, the mass ratio of the waste organic resources to the decomposing agent is (98-99.8):(0.2-2.0).

Preferably, the waste organic resources include two or more of mushroom dregs, tea dregs, bean dregs, poultry manure, bran, rice bran, fruit dregs and seaweed dregs.

Preferably, the preparation method steps of described bio-organic fertilizer are as follows:

Mixing waste organic resources with a decomposing agent to obtain a mixed material; the C/N of the mixed material is (25-30): 1; the water content of the mixed material is 50-65%;

performing aerobic fermentation and anaerobic fermentation on the mixed material in turn to obtain an organic carrier;

The organic carrier is mixed with functional bacteria liquid to obtain bio-organic fertilizer; the functional bacteria liquid is obtained by sequentially performing strain activation, primary fermentation, secondary fermentation and tertiary fermentation by functional bacteria.

Preferably, the number of effective viable bacteria in the compound bio-organic fertilizer is more than or equal to 20 million/g.

The present invention also provides a method for preparing the compound bio-organic fertilizer described in the above technical solution, comprising: mixing the bio-organic fertilizer with a water-retaining agent to obtain the compound bio-organic fertilizer.

The present invention also provides an application of a water-retaining agent as a compound bio-organic fertilizer raw material to improve the fertilizer retention capacity and/or bio-fertilizer efficiency of the bio-organic fertilizer, and the water-retaining agent is a polyacrylate water-retaining agent.

The present invention also provides an application of a compound bio-organic fertilizer added with the water-retaining agent described in the above technical scheme in one or more of enhancing the bio-fertilizer effect, fertilizer retention capacity and promoting crop growth of the bio-organic fertilizer; The organic fertilizer includes a bio-organic fertilizer and a water-retaining agent, and the mass ratio of the bio-organic fertilizer to the water-retaining agent is (100-500):1.

Preferably, the enhancement of biological fertilizer efficiency and fertilizer retention capacity of the bio-organic fertilizer includes increasing the activity of functional bacteria in compacted red soil and/or enhancing the fertilizer retention of compacted red soil.

The invention provides a composite bio-organic fertilizer, which comprises a bio-organic fertilizer and a water-retaining agent, and the mass ratio of the bio-organic fertilizer to the water-retaining agent is (100-500):1. The composite bio-organic fertilizer provided by the present invention combines bio-organic fertilizer and water-retaining agent, improves the fertilizer-retaining ability of bio-organic fertilizer through water-retaining agent, thereby promoting crop growth; by controlling the ratio of bio-organic fertilizer and water-retaining agent, promotes bio-organic The functional bacteria in the fertilizer exert fertilizer effect after application, improving the biological fertilizer efficiency of the functional bacteria. The composite bio-organic fertilizer obtained by adding the water-retaining agent in the present invention has the characteristics of convenient use, labor saving, and fertilizer-increasing effect. The invention provides the application of a compound bio-organic fertilizer with water retention function in improving the activity of functional bacteria in compacted soil and/or enhancing the fertilizer retention of compacted soil. The compound bio-organic fertilizer obtained by compounding the bio-organic fertilizer and water-retaining agent In compacted soil, it can improve the condition of soil compaction, and at the same time, it can play a synergistic technical effect in improving the activity of functional bacteria in compacted red soil and enhancing the fertilizer conservation effect of compacted red soil. The experimental results of the examples show that after 15 days of application of the compound bio-organic fertilizer provided by the invention, the soil does not compact, and is still in a moist, loose state; the decline in the number of functional bacteria is slowed down; the soil cation exchange capacity can reach 26.9cmol/kg, The ability to retain fat is improved.

Description of drawings

Fig. 1 is the comparison figure of the soil appearance on the 1st day and the 15th day of cultivating in Processing 1 in the present invention;

Fig. 2 is the control figure of soil appearance on the 1st day and the 15th day of cultivating in processing 2 in the present invention;

Fig. 3 is the control figure of the soil appearance of cultivating the 1st day and the 15th day in the control 1 of the present invention;

Fig. 4 is the control figure of soil appearance on the 1st day and the 15th day of cultivating in comparative example 1 in the present invention;

Fig. 5 is the control figure of soil appearance on the 1st day and the 15th day of cultivating in the treatment 3 in the present invention;

Fig. 6 is the control figure of soil appearance on the 1st day and the 15th day of cultivating in the treatment 4 in the present invention;

Fig. 7 is the control figure of the appearance of the soil on the 1st day and the 15th day of cultivation in the control 2 of the present invention;

Fig. 8 is a comparative picture of soil appearance on the first day and the fifteenth day of cultivation in Comparative Example 2 of the present invention.

Biological Deposit Instructions

Jelly-like Bacillus (Bacillus licheiformis), the preservation place is the General Microbiology Center of the China Committee for the Collection of Microbial Cultures, the preservation number is CGMCC No.3995, and the preservation time is October 27, 2010;

Bacillus subtilis (Bacillus subtilis), preserved at the General Microbiology Center of the China Committee for the Collection of Microorganisms, with the preservation number CGMCC NO.10248, and the preservation time was December 29, 2014.

Address of Preservation Unit: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing

Detailed ways

The invention provides a compound bio-organic fertilizer with water-retaining function, which comprises a bio-organic fertilizer and a water-retaining agent, and the mass ratio of the bio-organic fertilizer and the water-retaining agent is (100-500):1.

The compound bio-organic fertilizer provided by the invention includes bio-organic fertilizer. In the present invention, the bio-organic fertilizer preferably includes an organic carrier and functional bacteria. In the present invention, the number of effective viable bacteria of the functional bacteria in the bio-organic fertilizer is preferably 0.2-100 million/g.

In the present invention, the organic carrier is preferably obtained by fermenting and decomposing waste organic resources under the action of a decomposing agent.

In the present invention, the mass ratio of the waste organic resources and decomposing agent is preferably (98-99.8):(0.2-2.0), more preferably (98.5-99.5):(0.5-1.5), most preferably 99: 1.0.

In the present invention, the waste organic resources preferably include two or more of mushroom dregs, tea dregs, bean dregs, poultry manure, bran, rice bran, fruit dregs and seaweed dregs. The invention uses waste organic resources as the principle of preparing organic carriers, and can realize recycling and reuse of waste while providing organic matter. The present invention has no special limitation on the types and amounts of the components in the waste organic resources, as long as the ratio can meet the usage requirements according to the types of raw materials.

In the present invention, the decomposing agent is preferably the straw decomposing bacterial agent disclosed in Chinese patent (CN 104651277A: A straw decomposing bacterial agent and its preparation method and application). The invention adopts the patented decomposing bacteria agent, which can ensure the decomposing and harmless of the organic carrier.

In the present invention, there is no special limitation on the source of the organic carrier, and it can be prepared by using commercially available products or according to the method of fermenting and decomposing the organic carrier well known to those skilled in the art. In the present invention, the preparation method of the organic carrier preferably includes the following steps:

(1) Mixing waste organic resources with a decomposing agent to obtain a mixed material;

(2) Fermenting the mixed material obtained in the step (1) to obtain an organic carrier.

In the present invention, the waste organic resources are preferably mixed with the decomposing agent to obtain the mixed material. In the present invention, the C/N of the mixed material is preferably (25-30):1, more preferably (26-28):1; the water content of the mixed material is preferably 50-65%, more preferably 55-60%. The present invention controls the C/N and water content of the mixed material within the above range, which is more suitable for fermenting bacteria to play a role.

After obtaining the mixed material, in the present invention, the mixed material is preferably fermented to obtain an organic carrier. In the present invention, the fermentation preferably includes aerobic fermentation and anaerobic fermentation carried out in sequence.

In the present invention, the aerobic fermentation is preferably carried out under the condition of aeration; in the present invention, the material is preferably fully aerated with oxygen by turning and throwing. In the present invention, the temperature of the aerobic fermentation is preferably 25-65°C, more preferably 30-62°C, most preferably 35-60°C; the time of the aerobic fermentation is preferably 25-40 days, more preferably Preferably it is 30 to 35 days.

In the present invention, the anaerobic fermentation is preferably composting fermentation. In the present invention, the temperature of the anaerobic fermentation is preferably 25-35°C, more preferably 28-32°C; the time of the anaerobic fermentation is preferably 30-60 days, more preferably 40-50 days.

The present invention adopts the above preparation method to prepare the organic carrier, strictly controls the fermentation parameters in the fermentation process, and can further ensure that the organic carrier is decomposed and harmless.

In the present invention, the functional bacteria preferably include Paenibacillus jellylike and/or Bacillus subtilis. In an embodiment of the present invention, the preservation place of the jelly-like Paenibacillus (Bacillus licheiformis) is the General Microbiology Center of China Committee for the Preservation of Microorganisms, the preservation number is CGMCC No.3995, and the preservation time is October 2010 27th. In the present invention, the Bacillus subtilis is stored at the General Microorganism Center of the China Committee for the Collection of Microorganisms, with a storage number of CGMCCNO.10248 and a storage time of January 20, 2015.

The preparation method of the bio-organic fertilizer in the present invention has no special limitation, it only needs to mix the organic carrier and the functional bacteria. In the present invention, the functional bacteria are preferably mixed with the organic carrier in the form of functional bacteria liquid. The present invention has no special limitation on the ratio of the organic carrier and the functional bacteria solution, and it is sufficient to ensure that the effective number of viable bacteria of the obtained bio-organic fertilizer is 0.2-100 million/g.

In the present invention, the preparation of the functional bacteria liquid preferably includes: sequentially performing strain activation, primary fermentation, secondary fermentation and tertiary fermentation on the functional bacterial strains to obtain the functional bacterial liquid.

In the present invention, the functional bacteria strains are preferably activated to obtain activated strains. In the present invention, the activation of the strains preferably includes: inoculating the functional strains into an activation medium for activation to obtain activated strains. In the present invention, the activation temperature is preferably 28-30°C, more preferably 29°C; the activation time is preferably 24-28h, more preferably 26h.

In the present invention, the activation medium of the jelly-like Paenibacillus is preferably a solid medium, and the composition of the solid medium preferably includes: glucose 10-15 g/L, dipotassium hydrogen phosphate 0.3-0.5 g/L , magnesium sulfate 0.2-0.4g/L, sodium chloride 0.2-0.3g/L, calcium carbonate 1-1.5g/L and agar powder 20-25g/L. In the present invention, the initial pH value of the activated-grade culture medium of Paenibacillus jelly-like is preferably 7.0-7.5.

In the present invention, the activation medium of Bacillus subtilis preferably includes: glucose 10-15 g/L, peptone 8-10 g/L, sodium chloride 5-10 g/L and calcium carbonate 0.5-1 g/L. In the present invention, the initial pH value of the activation medium of Bacillus subtilis is preferably 7.0-7.5.

After the activated strain is obtained, the present invention preferably performs primary fermentation on the activated strain to obtain a primary fermentation liquid. In the present invention, the primary fermentation preferably includes: inoculating the activated bacteria into the primary medium for cultivation to obtain the primary fermentation liquid. In the present invention, the temperature of the primary fermentation is preferably 28 to 30°C, more preferably 29°C; the time of the primary fermentation is preferably 45 to 50h, more preferably 48h; the primary fermentation is preferably shaking Bottles of fermentation.

In the present invention, the primary medium of the jelly-like Paenibacillus is preferably a solid medium, and the composition of the solid medium preferably includes: sucrose 12～16g/L, dipotassium hydrogen phosphate 0.4～0.8g/L L. Magnesium sulfate 0.3-0.5g/L, sodium chloride 0.2-0.3g/L, calcium carbonate 2.0-3.0g/L and agar powder 20-25g/L. In the present invention, the initial pH value of the primary culture medium of Paenibacillus jelly-like is preferably 7.0-7.5.

In the present invention, the primary culture medium of Bacillus subtilis preferably includes: 12-18 g/L sucrose, 8-10 g/L peptone, 5-10 g/L sodium chloride and 0.5-1 g/L calcium carbonate. In the present invention, the initial pH value of the primary culture medium of Bacillus subtilis is preferably 7.0-7.5.

After the primary fermentation liquid is obtained, the present invention preferably performs secondary fermentation on the primary fermentation liquid to obtain a secondary fermentation liquid. In the present invention, the secondary fermentation preferably includes: inoculating the primary fermentation liquid into the secondary medium for cultivation to obtain the secondary fermentation liquid. In the present invention, the temperature of the secondary fermentation is preferably 28-30°C, more preferably 29°C; the time of the secondary fermentation is preferably 48-72h, more preferably 54-66h; the secondary fermentation Preference is given to seed tank fermentation.

In the present invention, the secondary culture medium of Paenibacillus jelly-like preferably includes: starch 5.5-9g/L, yeast powder 1.6-2.0g/L, soybean meal powder 6-8g/L, magnesium sulfate 1-1.4 g/L, dipotassium hydrogen phosphate 2~3g/L, calcium carbonate 5.5~8.5g/L and sodium chloride 0.2~0.3g/L. In the present invention, the initial pH value of the secondary culture medium of Paenibacillus jelly-like is preferably 7.0-7.5.

In the present invention, the secondary culture medium of Bacillus subtilis preferably includes: brown sugar 7-12g/L, starch 2-2.5g/L, soybean meal powder 10-12g/L, potassium dihydrogen phosphate 2-3g/L , dipotassium hydrogen phosphate 1~2g/L, calcium carbonate 0.5~1g/L, magnesium sulfate 0.5~1g/L, calcium chloride 0.5~1g/L, manganese sulfate 0.2~0.5g/L and ferric chloride 0.01~ 0.05g/L. In the present invention, the initial pH value of the secondary culture medium of Bacillus subtilis is preferably 7.0-7.5.

After the secondary fermentation broth is obtained, the present invention preferably performs the tertiary fermentation on the secondary fermentation broth to obtain the functional bacteria liquid. In the present invention, the tertiary fermentation preferably includes: inoculating the secondary fermentation liquid into the tertiary medium for fermentation to obtain a functional bacterial liquid. In the present invention, the inoculation amount of the secondary fermentation liquid is preferably 4% to 6% of the quality of the tertiary medium, more preferably 5%; the tertiary fermentation is preferably fermenter fermentation; the tertiary fermentation The temperature is preferably 30-35°C, more preferably 32°C; the time for the tertiary fermentation is preferably 3-5 days, more preferably 4 days. In the present invention, the process of the three-stage fermentation is preferably aeration, and the way of aeration is preferably: within the first 24 hours of fermentation, the aeration rate is 10-20 m ³ /h; after 24 hours of fermentation, the aeration rate is 15-24 m ³ /h. In the present invention, the tertiary fermentation is preferably carried out under agitation; the frequency of agitation is preferably 1-2 times/h, and the duration of each agitation is preferably 10-20 minutes.

In the present invention, the tertiary medium of each strain preferably has the same components as the secondary medium of the corresponding strain.

The composite bio-organic fertilizer provided by the invention includes a water-retaining agent. In the present invention, the water-retaining agent is preferably a polyacrylate-based water-retaining agent, and more preferably includes ammonium polyacrylate and/or potassium polyacrylate. In the present invention, the water-retaining agent can absorb nutrient elements soluble in water and be used in combination with bio-organic fertilizers to improve the fertilizer-retaining ability of bio-organic fertilizers; moreover, the water-retaining agent has the functions of water retention, heat preservation, and loose soil structure After the compound bio-organic fertilizer containing water-retaining agent is applied to the soil, the water-retaining performance of the water-retaining agent can maintain the humidity of the bio-organic fertilizer, and the porosity can enhance the air permeability of the bio-organic fertilizer, creating a more suitable growth environment for functional bacteria, especially in In areas with high temperature and drought and soil compaction, the thermal insulation performance can reduce the temperature difference between day and night of bio-organic fertilizers, improve the reproductive capacity and biological activity of microorganisms, and enhance the bio-fertilizer efficiency of bio-organic fertilizers, thereby improving soil fertility.

In the present invention, the mass ratio of the bio-organic fertilizer to the water-retaining agent is (100-500):1, preferably (200-300):1. In the present invention, the mass ratio of the bio-organic fertilizer and the water-retaining agent is within the above range, and the suitable humidity, porosity and thermal insulation performance of the bio-organic fertilizer can be maintained after being applied to the soil, and the reproductive capacity and biological activity of microorganisms can be improved. Enhance the bio-fertilizer effect of bio-organic fertilizer.

In the present invention, the number of effective viable bacteria in the compound bio-organic fertilizer is preferably ≥ 0.2 billion/g, more preferably ≥ 0.5 billion/g. In the present invention, the composite bio-organic fertilizer meets the technical indicators required by NY 884-2012: the number of effective viable bacteria ≥ 0.2 billion/g, organic matter ≥ 40%, moisture ≤ 30%, pH value 5.5-8.5, fecal coliform The number of groups is ≤100/g, and the death rate of roundworm eggs is ≥95%.

The compound bio-organic fertilizer provided by the invention includes bio-organic fertilizer and water-retaining agent, and the mass ratio of the bio-organic fertilizer and water-retaining agent is (100-500):1. The composite bio-organic fertilizer provided by the present invention combines bio-organic fertilizer and water-retaining agent, improves the fertilizer-retaining ability of bio-organic fertilizer through water-retaining agent, thereby promoting crop growth; by controlling the ratio of bio-organic fertilizer and water-retaining agent, promotes bio-organic The functional bacteria in the fertilizer exert fertilizer effect after application, improving the biological fertilizer efficiency of the functional bacteria.

The present invention also provides a method for preparing the compound bio-organic fertilizer described in the above technical solution, comprising: mixing the bio-organic fertilizer with a water-retaining agent to obtain the compound bio-organic fertilizer. The present invention has no special limitation on the mixing operation, and the technical scheme of mixing materials well known to those skilled in the art can be adopted. The preparation method of the compound bio-organic fertilizer provided by the invention is simple.

A compound bio-organic fertilizer provided by the present invention and its preparation method are described in detail below in conjunction with the examples, but they cannot be interpreted as limiting the protection scope of the present invention.

Example 1

Compound bio-organic fertilizer 1: The mass ratio of bio-organic fertilizer to potassium polyacrylate is 200:1, and contains 100 million Bacillus subtilis/g.

Bacillus subtilis liquid preparation method:

(1) Strain activation: the Bacillus subtilis strain was inoculated into the activation medium for activation, the activation temperature was 29° C., and the activation time was 26 h to obtain the activated strain. Activation medium: glucose 15g/L, peptone 10g/L, sodium chloride 8g/L and calcium carbonate 0.7g/L, initial pH value is 7.0.

(2) Primary fermentation: Inoculate the activated bacteria into the primary medium for cultivation to obtain the primary fermentation liquid. The temperature of the primary fermentation is 29° C., and the time of the primary fermentation is 48 hours. Primary fermentation medium: sucrose 16g/L, peptone 10g/L, sodium chloride 9g/L and calcium carbonate 0.8g/L, with an initial pH of 7.0.

(3) Secondary fermentation: inoculate the primary fermentation liquid into the secondary medium for cultivation, the fermentation temperature is 29°C, and the fermentation time is 60h to obtain the secondary fermentation liquid. Secondary medium: brown sugar 10g/L, starch 2g/L, soybean meal powder 11g/L, potassium dihydrogen phosphate 2g/L, dipotassium hydrogen phosphate 1g/L, calcium carbonate 1g/L, magnesium sulfate 0.9g/L, Calcium chloride 0.8g/L, manganese sulfate 0.4g/L and ferric chloride 0.02g/L, the initial pH value is 7.5.

(4) Tertiary fermentation: inoculate the secondary fermentation liquid into the tertiary medium for fermentation, the inoculum amount is 5% of the quality of the tertiary medium, the fermentation temperature is 32°C, and the ventilation volume in the first 24 hours of fermentation is ^15m3 /h; after 24 hours of fermentation, the ventilation rate is 18m ³ /h,

The stirring frequency is 2 times/h, each stirring is 15 minutes, and the fermentation time is 4 days to obtain the fermentation broth. The composition of the tertiary medium was the same as that of the secondary medium.

Compound bio-organic fertilizer preparation method:

Combine mushroom dregs, bean dregs, poultry manure, bran, and fruit dregs in a ratio of 3:2:3:1:1 to form an organic mixture. The material C/N is 25:1 and the water content is 55%. The material is fully Mix well. Put the decomposing agent evenly into the fermentation tank according to the ratio of mixed material: decomposing agent = 99:1. The decomposing agent comes from the patent product of Fujian Sanju Biotechnology Co., Ltd., and the patent name is "a straw decomposing bacterial agent and its preparation method and application”, the patent number is CN 104651277A. During the fermentation period, the material is regularly turned over by a turning machine, so that the material is fully aerated and fermented at 35-60 °C for 35 days, and then stacked out of the tank for anaerobic post-ripening fermentation at 29-32 °C for 40 days, so that the material is fully decomposed. organic carrier.

In terms of parts by mass, 2 parts of Bacillus subtilis bacterial liquid are mixed and adsorbed on 98 parts of organic carrier with a sprayer to make bio-organic fertilizer. In terms of parts by mass, 1 part of potassium polyacrylate is added to 200 parts of bio-organic fertilizer, and mixed to make composite bio-organic fertilizer 1.

Example 2

Compound bio-organic fertilizer 2: The mass ratio of bio-organic fertilizer to potassium polyacrylate is 300:1, and contains 100 million Bacillus subtilis/g.

The preparation method of Bacillus subtilis bacterium liquid and compound bio-organic fertilizer refers to "Example 1", the difference is: in parts by mass, 1 part of potassium polyacrylate is added to 300 parts of bio-organic fertilizer, and mixed to make compound bio-organic fertilizer 2.

Example 3

Compound bio-organic fertilizer 3: The mass ratio of bio-organic fertilizer to ammonium polyacrylate is 200:1, and contains 0.5 billion/g of Paenibacillus jelly-like bacteria.

Preparation method of jelly-like Paenibacillus bacterial liquid:

The process flow and process parameters of the jelly-like Paenibacillus bacterial liquid refer to the "Preparation method of the Bacillus subtilis bacterial liquid". The main difference between the two is that the composition of the medium is different. The medium composition of Paenibacillus jelly-like is as follows:

(1) Activation medium composition: glucose 12g/L, dipotassium hydrogen phosphate 0.3g/L, magnesium sulfate 0.3g/L, sodium chloride 0.2g/L, calcium carbonate 1.5g/L, agar powder 20g/L, The initial pH was 7.0.

(2) Primary fermentation medium composition: sucrose 14g/L, dipotassium hydrogen phosphate 0.6g/L, magnesium sulfate 0.4g/L, sodium chloride 0.3g/L, calcium carbonate 2.5g/L and agar powder 20g/L L, the initial pH value is 7.0.

(3) The composition of secondary and tertiary medium: starch 7.5g/L, yeast powder 1.8g/L, soybean meal powder 7g/L, magnesium sulfate 1.2g/L, dipotassium hydrogen phosphate 2.8g/L, calcium carbonate 6.5 g/L and sodium chloride 0.3g/L, the initial pH value is 7.5.

Compound bio-organic fertilizer preparation method:

Mushroom dregs, tea dregs, bean dregs, rice bran, and seaweed dregs form an organic mixture in a ratio of 2:2:2:1:3. The C/N ratio of the material is 28:1, and the water content is 55%. Mix the materials thoroughly. Put the decomposing agent evenly into the fermentation tank according to the ratio of mixed material: decomposing agent = 99.5:0.5. The decomposing agent comes from the patent product of Fujian Sanju Biotechnology Co., Ltd., and the patent name is "a straw decomposing bacterial agent and its preparation method and application", the patent number is CN 104651277 A. During the fermentation period, the material is regularly turned over by a turning machine, so that the material is fully aerated and fermented in the range of 35-65 °C for 40 days, and then stacked out of the tank for anaerobic post-ripening fermentation at 28-30 °C for 45 days, so that the material is fully decomposed and made organic carrier. In terms of parts by mass, 3 parts of the jelly-like Paenibacillus bacteria liquid are mixed and adsorbed on 97 parts of organic carriers with a sprayer to make bio-organic fertilizer. In parts by mass, add 1 part of ammonium polyacrylate to 200 parts of bio-organic fertilizer, and mix to make compound bio-organic fertilizer 3.

Example 4

Compound bio-organic fertilizer 4: The mass ratio of bio-organic fertilizer to ammonium polyacrylate is 300:1, and contains 50 million Paenibacillus jelly-like bacteria/g.

The preparation method of the jelly-like Paenibacillus bacterial liquid and the compound bio-organic fertilizer refers to "Example 3", the difference is that, in parts by mass, 300 parts of the bio-organic fertilizer is added to 1 part of ammonium polyacrylate, and mixed to form a compound Microbial fertilizer4.

Performance Testing

Effect of compound bio-organic fertilizer on soil physical and chemical properties and activity of functional bacteria (Bacillus subtilis, Paenibacillus jelly-like).

(1) Materials and methods

The test was carried out in the laboratory of "Sanju" Company in Nanjing County, Fujian Province, using constant temperature soil culture method. Mix the soil and the compound bio-organic fertilizer at a ratio of 1:1 to obtain a mixture, put it into a petri dish, add water to reach saturation, and cultivate it in a constant temperature incubator at 25°C for 15 days. The soil is taken from the local field. The soil type is red soil, which is in a compacted state. Air-dry, remove impurities, and grind through a 20-mesh sieve for later use.

The bio-organic fertilizer was used as a control. The properties of the bio-organic fertilizer 1 were the same as those of the compound bio-organic fertilizers 1 and 2, and the properties of the bio-organic fertilizer 2 were the same as those of the compound bio-organic fertilizers 3 and 4 except that it did not contain a water-retaining agent.

Bio-organic fertilizer + water-retaining agent is used as a comparison example, according to the mass ratio of (bio-organic fertilizer + water-retaining agent): soil = 1:1, the two are added to the soil in turn, mixed to obtain a mixture, and the cultivation method is the same as other treatments. The purpose of the comparative example is to compare the effects of bio-organic fertilizers and water-retaining agents after they are made into compound bio-organic fertilizers and the effects of step-by-step application of the two.

Treatment 1: compound bio-organic fertilizer 1+soil, the content of Bacillus subtilis in the mixture is 0.5 billion/g.

Treatment 2: compound bio-organic fertilizer 2+ soil, the content of Bacillus subtilis in the mixture is 0.5 billion/g.

Control 1: bio-organic fertilizer 1+soil, the content of Bacillus subtilis in the mixture is 50 million/g.

Comparative example 1: bio-organic fertilizer 1+potassium polyacrylate+soil, the mass ratio of bio-organic fertilizer 1 to potassium polyacrylate is 200:1, and the content of Bacillus subtilis in the mixture is 50 million/g.

Treatment 3: compound bio-organic fertilizer 3+ soil, the jelly-like Paenibacillus content of the mixture is 25 million/g.

Treatment 4: compound bio-organic fertilizer 4+ soil, the jelly-like Paenibacillus content of the mixture is 0.25 billion/g.

Control 2: bio-organic fertilizer 2+soil, the jelly-like Paenibacillus content of the mixture is 25 million/g.

Comparative example 2: bio-organic fertilizer 2+ammonium polyacrylate+soil, the mass ratio of bio-organic fertilizer 2 to ammonium polyacrylate is 200:1, and the jelly-like Paenibacillus content of the mixture is 25 million/g.

(2) Test management

Observe changes in soil state, detect changes in the activity of functional bacteria and soil fertility.

(3) Test results

Observe the soil state in Treatments 1-4, Controls 1 and 2, and Comparative Examples 1 and 2, and obtain the appearance of the soil on the first day and 15th day of cultivation for each treatment method, as shown in Figures 1-8 respectively.

It can be seen from Figures 1 to 8 that on the first day of culture, when water was added to saturation, the bio-organic fertilizer treatment (control 1, control 2) was in a viscous state, and obvious water traces were visible. After 15 days of culture, hardening occurred, and the water was almost evaporated and lost. The soil is relatively dry; however, the compound bio-organic fertilizer treatment (treatments 1-4) has less water loss, can lock water in the mixture, and keep it in a loose state until the 15th day, without hardening, and the soil is still in a moist state . Bio-organic fertilizer + water-retaining agent treatment (comparative example 1, comparative example 2) improved water retention effect compared with bio-organic fertilizer treatment, but not as obvious as compound bio-organic fertilizer treatment. It shows that the compound bio-organic fertilizer prepared by bio-organic fertilizer and water-retaining agent can significantly loosen the soil, ventilate, and retain water. However, the bio-organic fertilizer and water-retaining agent are applied to the soil step by step, and the amount of water-retaining agent is small, which is difficult to be compared with The bio-organic fertilizer is evenly mixed to reduce the water-retaining function of the water-retaining agent.

The number of functional bacteria was detected in the soil in treatments 1-4, controls 1 and 2, and comparative examples 1 and 2, and the number of functional bacteria on the first day and the fifteenth day of culture was shown in Table 1.

Table 1 Effects of different treatments on the activity of functional bacteria

deal with bacteria Initial bacterial count (100 million/g) The number of bacteria after 15 days of culture (100 million/g) processing 1 Bacillus subtilis 0.5 0.49 processing 2 Bacillus subtilis 0.5 0.48 Control 1 Bacillus subtilis 0.5 0.45 Comparative example 1 Bacillus subtilis 0.5 0.46 processing 3 Paenibacillus jelly-like 0.25 0.24 Process 4 Paenibacillus jelly-like 0.25 0.22 Control 2 Paenibacillus jelly-like 0.25 0.20 Comparative example 2 Paenibacillus jelly-like 0.25 0.20

It can be seen from Table 1 that the number of functional bacteria showed a downward trend after adding bio-organic fertilizers (control 1 and control 2) to the soil, and the treatment of compound bio-organic fertilizer prepared by adding water-retaining agent could delay the decline. Application (comparative example 1, comparative example 2) has obvious effects, and the effect of 200:1 addition (treatment 1, treatment 3) is more obvious. The growth of functional bacteria requires suitable humidity and oxygen. It contains compound bio-organic fertilizers and water-retaining agents that are fully mixed. After being applied to the soil, it can better loosen the soil, enhance air permeability, and retain water. It is suitable for the reproduction and growth of functional bacteria. So as to maintain a high biological activity.

The cation exchange capacity test was carried out on the 15th day of soil culture in Treatments 1-4, Controls 1 and 2, and Comparative Examples 1 and 2, and the results are shown in Table 2.

Table 2 Effects of different treatments on soil cation exchange capacity

deal with processing content Cation exchange capacity (cmol/kg) processing 1 Compound bio-organic fertilizer 1+ soil 27.2 processing 2 Compound bio-organic fertilizer 2+ soil 25.1 Control 1 Bio organic fertilizer 1+ soil 18.6 Comparative example 1 Bio-organic fertilizer 1+potassium polyacrylate+soil 19.9 processing 3 Compound bio-organic fertilizer 3+ soil 26.9 Process 4 Compound bio-organic fertilizer 4+ soil 26.2 Control 2 Bio-organic fertilizer 2+ soil 18.5 Comparative example 1 Bio-organic fertilizer 2 + ammonium polyacrylate + soil 20.5

The size of the cation exchange capacity (CEC) basically represents the amount of nutrients that the soil may hold, that is, the level of fertility. As can be seen from Table 2, with respect to bio-organic fertilizer treatment (contrast 1, contrast 2), compound bio-organic fertilizer treatment (treatment 1～treatment 4) obviously improves soil cation exchange capacity, strengthens soil fertility, compares bio-organic fertilizer and water retention The effects of step-by-step application (comparative example 1, comparative example 2) are obvious, and the effect of adding 200:1 (treatment 1, treatment 3) is more obvious. This is because the water-retaining agent and bio-organic fertilizer Fully mixed and evenly distributed water-retaining agent can absorb nutrients in the soil solution, thereby enhancing the fertilizer-retaining effect of the compound bio-organic fertilizer.

As can be seen from the above comparative examples and examples, the compound bio-organic fertilizer provided by the present invention combines the bio-organic fertilizer with the water-retaining agent, which can effectively prevent soil compaction, improve the growth environment of functional bacteria in the fertilizer, make it play a good role, and improve Bio-organic fertilizer, and has a good fertilizer effect.

The above descriptions are only preferred embodiments of the present invention, and do not limit the present invention in any form. It should be pointed out that those skilled in the art can make some improvements and modifications without departing from the principle of the present invention, and these improvements and modifications should also be regarded as the protection scope of the present invention.

Claims (10)

1. The application of a water-retaining agent as a compound bio-organic fertilizer raw material to improve the fertilizer retention capacity and/or biological fertilizer effect of the bio-organic fertilizer, wherein the water-retaining agent is a polyacrylate water-retaining agent. 2. A compound bio-organic fertilizer adding the water-retaining agent described in claim 1 is applied in one or more of the bio-fertilizer effect, fertilizer retention capacity and promotion of crop growth of the bio-organic fertilizer; The compound bio-organic fertilizer includes a bio-organic fertilizer and a water-retaining agent, and the mass ratio of the bio-organic fertilizer to the water-retaining agent is (100-500):1. 3. The application according to claim 2, characterized in that, said enhancement of the bio-fertilizer efficiency and fertilizer retention capacity of the bio-organic fertilizer comprises improving the activity of functional bacteria in compacted red soil and/or enhancing the fertilizer retention of compacted red soil. 4. The application according to claim 2, characterized in that, the mass ratio of the bio-organic fertilizer to the water-retaining agent is (200-300):1. 5. The application according to claim 2, wherein the water retaining agent comprises ammonium polyacrylate and/or potassium polyacrylate. 6. application according to claim 2, is characterized in that, described bio-organic fertilizer comprises organic carrier and functional bacterium; Described functional bacterium comprises jelly-like Paenibacillus and/or Bacillus subtilis; The preparation method step of described biological organic fertilizer is as follows: Mixing waste organic resources with a decomposing agent to obtain a mixed material; the C/N of the mixed material is (25-30):1; the water content of the mixed material is 50-65%; performing aerobic fermentation and anaerobic fermentation on the mixed material in turn to obtain an organic carrier; The organic carrier is mixed with functional bacteria liquid to obtain bio-organic fertilizer; the functional bacteria liquid is obtained by sequentially performing strain activation, primary fermentation, secondary fermentation and tertiary fermentation by functional bacteria. 7. The application according to claim 6, characterized in that, the organic carrier is obtained by fermenting and decomposing waste organic resources under the action of a decomposing agent; the mass ratio of the waste organic resources and decomposing agent is (98～99.8 ): (0.2～2.0). 8. The application according to claim 7, characterized in that the waste organic resources include two or more of mushroom dregs, tea dregs, bean dregs, poultry manure, bran, rice bran, fruit dregs and seaweed dregs. 9. The application according to claim 1, characterized in that, the number of effective viable bacteria in the compound bio-organic fertilizer is more than or equal to 0.2 billion/g. 10. The application according to claim 1, the preparation method of the compound bio-organic fertilizer comprises: mixing the bio-organic fertilizer with a water-retaining agent to obtain the compound bio-organic fertilizer.

Images