CN105481510B - The green organic implantation methods of fruit - Google Patents

The green organic implantation methods of fruit Download PDF

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CN105481510B
CN105481510B CN201510988006.9A CN201510988006A CN105481510B CN 105481510 B CN105481510 B CN 105481510B CN 201510988006 A CN201510988006 A CN 201510988006A CN 105481510 B CN105481510 B CN 105481510B
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CN105481510A (en
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陈龙弟
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CHANGZHOU WANGZHE QINGCHENG ECOLOGY SIGHTSEE CO., LTD.
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Changzhou Wangzhe Qingcheng Ecology Sightsee Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B1/00Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
    • C05B1/02Superphosphates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C21/00Methods of fertilising, sowing or planting
    • A01C21/005Following a specific plan, e.g. pattern
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F3/00Fertilisers from human or animal excrements, e.g. manure
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor

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Abstract

The invention discloses a kind of green organic implantation methods of fruit, including field planting, fertilising and watering step, which is characterized in that the fertilising step uses organic-biological compound fertilizer;Organic-biological compound fertilizer is prepared by following raw materials: 78-82 parts of organic fertilizer, 8-12 parts of inorganic fertilizer, 1-3 parts of liquid microbe microbial inoculum;The organic fertilizer is prepared by the raw material of following weight percentage: enzyme live keeping agent 1-3%, 10-20% rice husk, 15-25% Sunflower Stalk, 10-20% sawdust, 5-15% cow dung, 5-15% chicken manure, surplus is water.The enzyme live keeping agent is mixed by trehalose, mannitol, maltitol.The green organic implantation methods of fruit of the invention, for the organic implantation methods of green ecological, it follows the course of nature, the method for taking farming, physics, chemistry and biology comes fertilizing soil, prevention and treatment pest and disease damage, the bio-diversity and benign cycle of the plantation ecosystem are maintained and established, green fruit of safety, high yield is obtained.

Description

The green organic implantation methods of fruit
Technical field
The present invention relates to plants to cultivate field of planting, and in particular to a kind of green organic implantation methods of fruit.
Background technique
Green fruit (also known as pecan tree, Longevity) belongs to the hickory also known as apocarya or Chang Mount of Juglandaceae Walnut, the entitled pecan or hickory of English, green fruit fruit type is such as big olive-shaped, and it is one of big nut in the world ten that meat is more and fragrant. Green fruit shape is the dry fruit of long ellipse, and the U.S. and northern Mexico for originating in North American continent have become international dry fruit One of class tree species, kernel have excellent Dietotherapy health value.
High-quality green pericarp is very thin, can be peeled off with hand, the hickory nut and big walnut that taste circle of kernel is often eaten in us Between, mouthfeel is aromatic crisp to make us being profuse in praise.It is one of 17 kinds of hickory nut in the whole world, belongs to pure wild fruit, is collection mountainous region Anima feed, the natural green food of no any public hazards pollution.
Record on ancient book: brain is led in Longevity energy kidney tonifying, beneficial to wisdom.Long-lived kernel and rock sugar are pounded mud altogether, treasure tank In, two spoons are taken every time, and boiled water Instant Drinks, the one layer of white liquid floated after that punching is the strongest long-lived fruit milk of cerebrum tonifying power.
The suitable soil of green fruit is best with rendizina, mountain yellow soil, followed by by shale development yellow-red soil and Terra rossa, and childhood rendzinas and development on granite yellow-red soil and Quaternary Red Soil growth result it is poor, without changing It is good to be not suitable for developing hickory nut.It is required that soil subacidity, to neutrality, quality is glued by light earth to light, and bulk density is less than 1.35, organic matter Greater than 1.5%, soil thickness is in 50cm or more.Molybdenum, copper, iron it is required that validity phosphorus potassium content is higher in soil, and in soil, Manganese, boron, nickel, cobalt, chromium, selenium and other trace elements content determine or constrain the distribution of hickory nut, affect hickory nut growth, Development and normal outcome.
Green fruit is relatively cold-resistant, shade tolerant, and growth district is with 13.5-17.2 DEG C of average annual temperature, annual rainfall 1300-1500mm, 200-800 meters of height above sea level of Schattenseite mountainous region is preferably.
Because soil fertility is the dominant factor for influencing green fruit growth and development, and nutritional condition is the master for causing biennial bearing Inducement, so first is that it is however emphasized that the application of biological organic fertilizer in fertilizer application;Second is that focus on the application of phosphorus potash fertilizer;Third is that wanting Pay attention to the application of foliar fertilizer.
Soil fertility is the essential attribute and substantive characteristics of soil, is that nutrient, water are supplied for plant growth and coordinated to soil Divide, the ability of air and heat, is the combined reaction of soil physics, chemistry and biology property.Four big Fertility evolutions have: nutrition Factor: nutrient, moisture;Environmental condition: air, heat.
Soil fertility is the general performance and soil difference of soil physics, chemistry, biochemistry and physicochemical characteristics In the intrinsic propesties of matrix.The economic fertility to be formed is combined including natural fertility, artificial fertility and the two.
Integrated use organic fertilizer, inorganic fertilizer and microbial manure of the present invention have invented a kind of organic implantation methods of green fruit.
Summary of the invention
For the deficiencies in the prior art, technical problem to be solved by the invention is to provide a kind of green fruit is organic Implantation methods.
Object of the present invention is to what is be achieved through the following technical solutions:
A kind of green organic implantation methods of fruit (the also known as organic implantation methods of hickory nut), including field planting, fertilising and watering step Suddenly, the fertilising step uses organic-biological compound fertilizer.
The organic-biological compound fertilizer, is prepared by the raw material of following weight parts: 78-82 parts of organic fertilizer, inorganic fertilizer 8- 12 parts, 1-3 parts of liquid microbe microbial inoculum.
The organic fertilizer is prepared by the raw material of following weight percentage: enzyme live keeping agent 1-3%, 10-20% rice husk, 15-25% Sunflower Stalk, 10-20% sawdust, 5-15% cow dung, 5-15% chicken manure, surplus are water.
The enzyme live keeping agent is mixed by trehalose, mannitol, maltitol, the trehalose, mannitol, malt The mass ratio of sugar alcohol is (1-3): (1-3): (1-3).
The inorganic fertilizer is composed of the following weight percentages of raw materials: 25-35wt% urea, 25-35wt% potassium sulfate, Surplus is calcium superphosphate.
The liquid microbe microbial inoculum is made of microbial bacterial agent and culture medium, potassium solubilizing bacteria in the liquid microbe microbial inoculum Inoculum concentration be (1-3) hundred million cfu/g culture medium, the inoculum concentration of phosphorus decomposing bacillus megaterium is (1-3) hundred million cfu/g culture medium, Gu The inoculum concentration of nitrogen bacterium is (1-3) hundred million cfu/g culture medium.
The culture medium is prepared by the raw material of following weight percentage: beef extract 0.4-0.6%, peptone 0.8- 1.2%, sodium chloride 0.4-0.6%, surplus are water;PH is 7.1-7.5.
The dose of the organic-biological compound fertilizer is that annual every plant of green fruit tree applies organic-biological compound fertilizer 10-30kg.
The present invention also provides the preparation methods of above-mentioned organic-biological compound fertilizer, comprising the following steps:
(1) enzyme live keeping agent is dissolved in water to be uniformly mixed, then is sprayed onto organic substrate and ferments, obtain organic fertilizer;
(2) microbial bacterial agent is inoculated into culture medium, obtains liquid microbe microbial inoculum;
(3) organic fertilizer, inorganic fertilizer, liquid microbe microbial inoculum are uniformly mixed.
Preferably, the temperature fermented in the step (1) is 60-75 DEG C, and the time is 45-60 days.
The green organic implantation methods of fruit of the invention are the organic implantation methods of green ecological, it then follows the natural law takes agriculture Work, physics, chemistry and biology method come fertilizing soil, prevention and treatment pest and disease damage, the biology for maintaining and establishing the plantation ecosystem is more Sample and benign cycle obtain the green organic implantation methods of fruit of safety, high yield;The present invention is from raw material sources and implantation methods It sees, non-toxic, harmless and pollution-free, implantation methods are simple and easy to do, and raw material resources are abundant, while also can solve because animal and plant residuum is stacked Caused by problem of environmental pollution.
Specific embodiment
The present invention will be further explained with reference to the examples below, as described below, is only to preferable implementation of the invention Example, not limits the present invention, any person skilled in the art is possibly also with the disclosure above Technology contents be changed to the equivalent embodiment changed on an equal basis.Without departing from the concept of the present invention, according to the present invention Technical spirit any simple modification or equivalent variations that following embodiment is made, fall within the scope of protection of the present invention.
Each raw material introduction of embodiment:
Trehalose, No. CAS: 99-20-7.
Mannitol, No. CAS: 69-65-8.
Maltitol, No. CAS: 585-88-6.
Potassium sulfate, No. CAS: 7778-80-5.
Calcium superphosphate, No. CAS: 10031-30-8
Urea, No. CAS: 57-13-6.
Sawdust, phosphorus content 46.13%, nitrogen content 0.3420%.
Rice husk, phosphorus content 44.89%, nitrogen content 0.8832%.
Sunflower Stalk, phosphorus content 41.38%, nitrogen content 0.4604%.
Cow dung, phosphorus content 35.38%, nitrogen content 2.2481%.
Chicken manure, phosphorus content 37.84%, nitrogen content 3.0563%.
Potassium solubilizing bacteria, Classification system: potassiumbacteria is that separate from soil a kind of can break up aluminium silicon The bacterium of hydrochlorate and phosphorus ash stone mineral can be used as microbial manure.
Phosphorus decomposing bacillus megaterium, Classification system: Bacillusmegatheriumvar.Phosphaticum is soil In one in the stronger bacterium of dissolving phosphoric acid compound ability, microbial manure can be used as.
Nitrogen-fixing bacteria, Classification system: LatinNameAzotobactersp.Thallus is rod-shaped, oval or spherical shape, sprouts without in Spore, Gram-negative.Stringent aerobic, organic nutrition type, the nitrogen being capable of fixing in air.
Green fruit grafting, the kind for using Luoning County Dong Lin nursery stock Co., Ltd to provide is the U.S. of wave Buddhist nun (Pawnee) Green fruit (hickory nut) grafting.
Embodiment 1
Organic-biological raw material of mix-compound fertilizer (parts by weight): 80 parts of organic fertilizer, 3 parts of urea, 3 parts of potassium sulfate, 4 parts of calcium superphosphate, 2 parts of liquid microbe microbial inoculum.
The raw material (weight percent) of above-mentioned organic fertilizer: 0.6% trehalose, 0.6% mannitol, 0.6% maltitol, 18% rice husk, 22% Sunflower Stalk, 18% sawdust, 13% cow dung, 13% chicken manure, surplus are water.
Liquid microbe microbial inoculum is made of microbial bacterial agent and culture medium, the inoculum concentration of potassium solubilizing bacteria in liquid microbe microbial inoculum For 200,000,000 cfu/g culture mediums, the inoculum concentration of phosphorus decomposing bacillus megaterium is 200,000,000 cfu/g culture mediums, and the inoculum concentration of nitrogen-fixing bacteria is 200,000,000 Cfu/g culture medium.
Culture medium raw material (weight percent): beef extract 0.5%, peptone 1.0%, sodium chloride 0.5%, water 98%;pH It is 7.3.
The preparation method of organic-biological compound fertilizer, comprising the following steps:
(1) enzyme live keeping agent trehalose, mannitol, maltitol are dissolved in water and are uniformly mixed, obtain enzyme live keeping agent aqueous solution, It is uniformly sprayed onto rice husk again, Sunflower Stalk, sawdust, cow dung, is uniformly mixed in chicken manure and ferments, the temperature of fermentation is 65 DEG C, hair The ferment time is 55 days, obtains the organic fertilizer.
(2) beef extract, peptone, sodium chloride are successively weighed by formula rate, is dissolved with hot water, are uniformly mixed, are used 1mol/ It is 7.3 that L sodium hydroxide or 1mol/L dilute hydrochloric acid, which adjust pH, by the culture medium prepared with 1.05kg/cm2, 121.3 DEG C, 20 points It is spare after clock high pressure steam sterilization.
Microbial bacterial agent potassium solubilizing bacteria, phosphorus decomposing bacillus megaterium, nitrogen-fixing bacteria are inoculated into culture medium according to a conventional method again, Every gram of inoculation of medium potassium decomposing bacterium amount is 200,000,000 cfu, and phosphorus decomposing bacillus megaterium amount is 200,000,000 cfu, and fixed nitrogen bacterium amount is 200,000,000 cfu, Obtain liquid microbe microbial inoculum.
(3) organic fertilizer, urea, potassium sulfate, calcium superphosphate, liquid microbe microbial inoculum are mixed by formula rate equal It is even, it can be prepared by organic-biological compound fertilizer of the invention.
The green organic implantation methods of fruit, comprising the following steps:
(1) green fruit grafting winter is colonized, choose level of ground water is higher, soil glues weight, pH value 7.3 it is flat Soil planted, planting density is 4 × 5m, and every seedling applies organic-biological compound fertilizer 10kg, watering to soil when plantation Water content is 40wt%.
(2) configuration of pollinizers configures corresponding pollinizers, pollinizers selection " wave west " according to green fruit flowering time (Posey), pollinizers and green fruit allocation ratio are 1:10.
(3) plant after, annual every plant of early and middle ten days in March is applied organic-biological compound fertilizer 10kg, in July every plant of the first tenday period of a month apply it is organic Biological compound fertilizer 10kg, holding soil moisture content are 40wt%.
Embodiment 2
Green fruit is planted according to method described in embodiment 1, difference is only in that: organic fertilizer raw material (weight percent): 0.9% trehalose, 0.9% mannitol, 18% rice husk, 22% Sunflower Stalk, 18% sawdust, 13% cow dung, 13% chicken manure, surplus are Water.
Embodiment 3
Green fruit is planted according to method described in embodiment 1, difference is only in that: organic fertilizer raw material (weight percent): 0.9% trehalose, 0.9% maltitol, 18% rice husk, 22% Sunflower Stalk, 18% sawdust, 13% cow dung, 13% chicken manure, surplus For water.
Embodiment 4
Green fruit is planted according to method described in embodiment 1, difference is only in that: organic fertilizer raw material (weight percent): 0.9% mannitol, 0.9% maltitol, 18% rice husk, 22% Sunflower Stalk, 18% sawdust, 13% cow dung, 13% chicken manure, surplus For water.
Comparative example 1
Green fruit is planted according to method described in embodiment 1, difference is only in that: organic fertilizer raw material (weight percent): 18% rice husk, 22% Sunflower Stalk, 18% sawdust, 13% cow dung, 13% chicken manure, surplus are water;Spray water is into organic substrate It ferments.
Test case 1
The 1st year after 1 long-term cropping of embodiment 1-4 and comparative example, the 2nd year, the 3rd year soil are sampled respectively, surveyed Enzyme Activities in Soils is tried, test method is as follows:
The measurement of soil invertase activity: weighing 5.00g fresh soil sample in 50mL triangular flask, adds 5 drop toluene, places Matrix (8% sucrose solution) and each 10mL of pH5.5 phosphate buffer solution are added after 15min, 37 DEG C of cultures for 24 hours, then use water It is settled to 50mL, is filtered after shaking up, while being arranged without matrix and without soil sample check experiment.Filtrate 0.5mL is taken to be injected into 50mL appearance In measuring bottle, 3ml3,5- dinitrosalicylic acid is added, the water-bath 5min in boiling water bath uses distilled water constant volume after cooling, shakes up, Colorimetric at spectrophotometer 508nm.Sucrase active indicates that unit is with the milligram number of glucose in rear 1g soil for 24 hours mgglucose·g-1·(24h)-1.Concrete outcome is shown in Table 1:
The measurement of desaturase activity in soil: taking 3.00g fresh soil sample in 50mL centrifuge tube, sequentially adds tri- hydroxyl of pH7.6 Aminomethane (Tris)-HC1 buffer, 0.1mol/L glucose solution, each 2mL of 0.5% red tetrazolium, are placed in 37 DEG C of perseverances It cultivates for 24 hours, while being arranged without matrix and without soil sample check experiment in incubator.The 2 drop concentrated sulfuric acids are added after taking-up, terminate reaction, so Afterwards be added 10mL toluene shaking, be centrifuged 5min at 4000rpm, take upper layer organic solvent at spectrophotometer 492nm into Row colorimetric.Dehydrogenase activity indicates with the micrograms of San Ben Ji formazan (TF) are generated in 1g soil after for 24 hours, unit mgTFg-1·(24h)-1.Concrete outcome is shown in Table 2:
The measurement of soil urease liveness: taking the fresh soil sample of 5.00g in 50mL triangular flask, puts after 1.0mL toluene is added 15min is set, 10mL10% urea liquid and 20mLpH6.7 citrate buffer solution then is being added, is shaking up, is cultivated at 37 DEG C 3h after culture, is settled to 50mL with 37 DEG C or so of distilled water, filters after shaking up, while being arranged without matrix and without soil sample Check experiment.It takes 1.0mL filtrate in 50mL volumetric flask, 20mL distilled water is added, then 4mL phenol sodium solution is added in oscillation Oscillation, immediately be added 3mL liquor natrii hypochloritis, sufficiently sway, constant volume after 20min, at the inherence spectrophotometer 578nm of 1h into Row colorimetric.Urease activity with after 3h 1g soil in NH3The milligram number of-N indicates, unit mgNH3-N·g-1·(3h)-1.Specifically It the results are shown in Table 3:
The measurement of soil neutral phosphatase activity: taking the fresh soil sample of 5.00g in 50mL triangular flask, and 1.0m toluene is added After place 15min, then add two sodium solution of 20mL0.5% benzenephosphonic acid (with pH8.5 trishydroxymethylaminomethane (Tris)- HC1 buffer) it shakes up, 3h is cultivated at 37 DEG C, and after culture, 20mL0.3%Al is added2(SO4)3Solution uses distilled water Constant volume filters after swaying, while being arranged without matrix and without soil sample check experiment.It takes 1.0mL filtrate in 50mL volumetric flask, is added 5mLpH8.5Tris-HC1 buffer solution, adds distilled water to 25mL, then the 5 drop bis- bromobenzene quinone chlorine acid imides of 2,6- are added in oscillation Reagent, constant volume after 20min place after 3h the colorimetric at spectrophotometer 578nm.Soil neutral phosphatase activity 1g soil after 3h In the milligram number of phenol that releases indicate, unit mghydroxybenzeneg-1·(3h)-1.Concrete outcome is shown in Table 4:
Table 1: soil invertase activity measurement result list position: mgglucoseg-1·(24h)-1
Embodiment 1 Embodiment 2 Embodiment 3 Embodiment 4 Comparative example 1
1st year 84.9 77.8 80.3 79.4 57.9
2nd year 86.5 78.3 81.1 80.2 55.9
3rd year 88.4 79.1 82.7 80.7 52.3
Table 2: desaturase activity in soil measurement result list position: mgTFg-1·(24h)-1
Embodiment 1 Embodiment 2 Embodiment 3 Embodiment 4 Comparative example 1
1st year 148.6 131.5 141.4 134.8 119.5
2nd year 162.7 139.1 152.3 145.9 123.2
3rd year 160.9 134.7 148.8 140.7 117.1
Table 3: soil urease liveness measurement result list position: mgNH3-N·g-1·(3h)-1
Table 4: soil neutral phosphatase activity measurement result list position: mghydroxybenzeneg-1·(3h)-1
Embodiment 1 Embodiment 2 Embodiment 3 Embodiment 4 Comparative example 1
1st year 1.02 0.80 0.92 0.83 0.55
2nd year 0.99 0.78 0.88 0.81 0.53
3rd year 1.09 0.81 0.86 0.82 0.49
Can significantly be found out by table 1-4: embodiment 1-4 has used after enzyme live keeping agent that main enzymatic activity is much higher than in soil (enzyme live keeping agent is not used) in comparative example 1, Major Enzymes in special embodiment 1 (trehalose, mannitol, maltitol compounding) soil Activity is apparently higher than embodiment 2-4 (trehalose, mannitol, any two kinds of compoundings in maltitol).
Test case 2
The 1st year after 1 long-term cropping of embodiment 1-4 and comparative example, the 2nd year, the 3rd year soil are sampled respectively, surveyed Organic carbon, alkali-hydrolyzable nitrogen, available phosphorus, effective potassium in soil are tried, test method is as follows:
The measurement of soil organic matter, alkali-hydrolyzable nitrogen, available phosphorus, effective potassium: soil organic matter uses potassium bichromate-Outside Heating Method Measurement;Soil alkali-hydrolyzable nitrogen is measured using alkaline hydrolysis diffusion method;Soil available phosphorus is measured using molybdenum antimony resistance colorimetric method;Available potassium in soils is adopted With ammonium acetate extraction-flame spectrophotometric determination, measuring method is referring to " Soil Analysis in Agricultural Chemistry " (Bao scholar denier 2008).
Harvest yield measurement: claim its weight after crop harvest.Concrete outcome is shown in Table 5-11:
Table 5: soil organic carbon list position: gkg-1
Embodiment 1 Embodiment 2 Embodiment 3 Embodiment 4 Comparative example 1
1st year 23.09 19.31 20.42 19.80 17.01
2nd year 22.14 18.17 19.12 18.57 15.54
3rd year 22.87 18.08 19.31 18.40 14.67
Table 6: soil available phosphorus content table unit: mgkg-1
Table 7: soil alkaline hydrolysis nitrogen content unit: mgkg-1
Embodiment 1 Embodiment 2 Embodiment 3 Embodiment 4 Comparative example 1
1st year 167.19 153.18 160.37 158.13 126.35
2nd year 164.27 144.92 156.15 149.93 112.28
3rd year 158.04 136.89 150.72 141.68 103.47
Table 8: available potassium in soils content table unit: mgkg-1
Embodiment 1 Embodiment 2 Embodiment 3 Embodiment 4 Comparative example 1
1st year 460.42 427.94 439.36 431.82 397.04
2nd year 443.16 401.52 416.43 407.37 379.12
3rd year 421.97 377.15 397.46 389.43 339.82
Table 9: third year crop harvests scale unit: kg after field planting
Table 10: the 4th year crop harvests scale unit: kg after field planting
Table 11: the 5th year crop harvests scale unit: kg after field planting
Can significantly be found out by table 5-8: embodiment 1-4 has used after enzyme live keeping agent organic carbon in soil, alkali-hydrolyzable nitrogen, has had Phosphorus, effective potassium content are imitated much higher than comparative example 1 (enzyme live keeping agent is not used), special embodiment 1 (trehalose, mannitol, malt Sugar alcohol compounding) organic carbon, alkali-hydrolyzable nitrogen, available phosphorus, effective potassium content are apparently higher than embodiment 2-4 (trehalose, sweet dew in soil Any two kinds of compoundings in alcohol, maltitol).
Can significantly be found out by table 9-11: embodiment 1-4 used after enzyme live keeping agent green fruit harvest yield much higher than pair (enzyme live keeping agent is not used) in ratio 1, and green fruit harvest yield of special embodiment 1 (trehalose, mannitol, maltitol compounding) is bright It is aobvious to be higher than embodiment 2-4 (trehalose, mannitol, any two kinds of compoundings in maltitol).These results suggest that enzyme live keeping agent is adopted The harvest yield of crop can be dramatically increased with trehalose, mannitol, maltitol three compounding.

Claims (2)

1. a kind of green organic implantation methods of fruit, including field planting, fertilising and watering step, which is characterized in that the fertilising step Using organic-biological compound fertilizer;
The organic-biological compound fertilizer, is prepared by the raw material of following weight parts: 78-82 parts of organic fertilizer, 8-12 parts of inorganic fertilizer, 1-3 parts of liquid microbe microbial inoculum.
The organic fertilizer is prepared by the raw material of following weight percentage: enzyme live keeping agent 1-3%, 10-20% rice husk, 15- 25% Sunflower Stalk, 10-20% sawdust, 5-15% cow dung, 5-15% chicken manure, surplus are water.
The enzyme live keeping agent is mixed by trehalose, mannitol, maltitol, the trehalose, mannitol, maltitol Mass ratio be (1-3): (1-3): (1-3).
The inorganic fertilizer is composed of the following weight percentages of raw materials: 25-35wt% urea, 25-35wt% potassium sulfate, surplus For calcium superphosphate.
The liquid microbe microbial inoculum is made of microbial bacterial agent and culture medium, and potassium solubilizing bacteria connects in the liquid microbe microbial inoculum Kind amount is (1-3) hundred million cfu/g culture medium, and the inoculum concentration of phosphorus decomposing bacillus megaterium is (1-3) hundred million cfu/g culture medium, nitrogen-fixing bacteria Inoculum concentration be (1-3) hundred million cfu/g culture medium.
The culture medium is prepared by the raw material of following weight percentage: beef extract 0.4-0.6%, peptone 0.8- 1.2%, sodium chloride 0.4-0.6%, surplus are water;PH is 7.1-7.5.
2. the green organic implantation methods of fruit as described in claim 1, which is characterized in that the fertilising of the organic-biological compound fertilizer Amount applies organic-biological compound fertilizer 10-30kg for annual every plant of green fruit tree.
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CN109400348A (en) * 2018-11-09 2019-03-01 中国农业科学院农业资源与农业区划研究所 A kind of alginic acid synergistic carrier preparation method of the urea suitable for winter wheat top dressing
CN109400349A (en) * 2018-11-09 2019-03-01 中国农业科学院农业资源与农业区划研究所 A kind of preparation method of the Humic acid synergistic carrier of the urea suitable for rice top dressing
CN109232097A (en) * 2018-11-09 2019-01-18 中国农业科学院农业资源与农业区划研究所 A kind of alginic acid synergistic carrier preparation method promoting urea conversion
CN109232101A (en) * 2018-11-09 2019-01-18 中国农业科学院农业资源与农业区划研究所 A kind of granular urea preparation method containing Humic acid synergistic carrier suitable for rice top dressing
CN109265255A (en) * 2018-11-09 2019-01-25 中国农业科学院农业资源与农业区划研究所 A kind of alginic acid synergistic carrier preparation method of the urea suitable for rice top dressing
CN109320378A (en) * 2018-11-09 2019-02-12 中国农业科学院农业资源与农业区划研究所 A kind of preparation method for the Humic acid synergistic carrier promoting urea conversion
CN109369251A (en) * 2018-11-09 2019-02-22 中国农业科学院农业资源与农业区划研究所 A kind of granular urea preparation method containing alginic acid synergistic carrier suitable for acid soil
CN109757520A (en) * 2019-01-25 2019-05-17 江苏大学 A kind of microorganism formulation and preparation method thereof promoting Limestone Region plant growth
CN113748908A (en) * 2021-08-17 2021-12-07 海南农佳佳科技发展有限公司 Green planting method for pecans
CN113854021A (en) * 2021-09-10 2021-12-31 海南农佳佳科技发展有限公司 Planting method of pecans

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1271014C (en) * 2004-11-26 2006-08-23 栖霞市宏翔微生物肥料有限公司 Multifunctional biological organic and inorganic blended fertilizer and its production process
CN102972253A (en) * 2012-12-19 2013-03-20 江苏省农业科学院 Facility high-yield cultivation technology for imping carya illinoensis
CN105077261B (en) * 2015-09-24 2017-09-22 江西果果生物科技有限公司 Compound fruit and vegetable ferment and preparation method thereof

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