CN105941111A - Dendrobium huoshanense C.Z.Tang et S.J.Cheng breeding method - Google Patents

Dendrobium huoshanense C.Z.Tang et S.J.Cheng breeding method Download PDF

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Publication number
CN105941111A
CN105941111A CN201610305097.6A CN201610305097A CN105941111A CN 105941111 A CN105941111 A CN 105941111A CN 201610305097 A CN201610305097 A CN 201610305097A CN 105941111 A CN105941111 A CN 105941111A
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sowing
former times
breeding method
tang
cheng
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陈昌全
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Anhui Shanzhencang Ecological Agriculture Co Ltd
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Anhui Shanzhencang Ecological Agriculture Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B1/00Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
    • C05B1/04Double-superphosphate; Triple-superphosphate; Other fertilisers based essentially on monocalcium phosphate
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention discloses a Dendrobium huoshanense C.Z.Tang et S.J.Cheng breeding method comprising the following steps: 1, harvesting ripened Dendrobium huoshanense C.Z.Tang et S.J.Cheng seeds, airing the seeds, and placing the seeds in shady and cool, dry and ventilating places for storage; 2, covering a movable shading net on top of a sowing greenhouse, covering a film on the surrounding of the sowing greenhouse, wherein the sowing bed is 35-55 cm away from the ground; 3, laying the planting matrix on the sowing bed using an asphalt felt cloth as a holder, thus obtaining a planting layer of 5-15 cm in thickness; 4, sowing the Dendrobium huoshanense C.Z.Tang et S.J.Cheng seeds on the planting layer, and watering the planting layer until the water content is 75-90wt%. The Dendrobium huoshanense C.Z.Tang et S.J.Cheng breeding method uses the Dendrobium huoshanense C.Z.Tang et S.J.Cheng seeds and the planting matrix to breed the seeds; the seeds can absorb the nutriments provided with the planting matrix so as to fast germinate, and thus growing into seedlings with stems and leaves. The method can improve seed germination capacity; in the wild plantation environment, the Dendrobium huoshanense C.Z.Tang et S.J.Cheng plantation is limited by various factors, like climate influence, and severe insect diseases; the Dendrobium huoshanense C.Z.Tang et S.J.Cheng breeding method can solve said problems, thus protecting Dendrobium huoshanense C.Z.Tang et S.J.Cheng breeding.

Description

A kind of breeding method of meter of dry measure used in former times
Technical field
The present invention relates to biological technical field, be specifically related to the breeding method of a kind of meter of dry measure used in former times.
Background technology
Rice dry measure used in former times, also known as: Herba Dendrobii, formal name used at school: Dendrobium huoshanense C.Z.Tang Et S.J.Cheng, for the perennial herbaceous plant that grows nonparasitically upon another plant of the orchid family, shape Qu Buzhi, like oxya chinensis thigh, It is the superfine product in meter dry measure used in former times, exclusive for the county, have original producton location geography protective emblem and endangered National level treasure rare Chinese medicine, be national wild secondary Chinese medicine protect kind, have and " plant Thing giant panda " title, Compendium of Material Medica record: rice dry measure used in former times is born between overhanging cliff, property is micro- Cold, there are nourishing the stomach to promote the production of body fluid, YIN nourishing heat extraction, the kidney invigorating and essence nourishing, effect of improving eyesight.With can open for tea Stomach spleen invigorating, Huoshan rice dry measure used in former times has beneficial essence and fills out in marrow, tonifying five ZANG-organs never sufficient, flat gastric qi, long muscle, Fructus Alpiniae Oxyphyllae relieving restlessness, clearind deficient heat, effect prolonged life of making light of one's life by commiting suicide, clear ZHAO Xue-Min, Zhao Xuekai " Compendium of Materia Medica Pick up any lost article from the road " " BAICAO warp " language says: have when rice dry measure used in former times is near a kind of shape short venerate cun permitted thin such as wick, color green grass or young crops Sweet in the mouth yellow, that chew, micro-have sliding saliva.
Have more than 1500 kind of Herba Dendrobii in the world, in state-owned 76 kinds of Herba Dendrobiis, Huoshan grows China Good Herba Dendrobii, 60,000 stockaded village's height above sea level 644 meters, hills and mountains cincture, vegetation is luxuriant, and long-term cloud and mist curls up, Weather is unique.Temperature on average is at 14.2-16.1 degree Celsius throughout the year, and warmer climate moistens, favorably Growth and dendrobium polysaccharide, effective accumulation of alkaloid component in Herba Dendrobii;Precipitation exists throughout the year Between 1100-1600mm, rainfall is abundant, is suitable for the growth characteristics that Herba Dendrobii happiness the moon happiness is wet.Secondly, 60000 stockaded villages have an area of tens inner hills and mountains cinctures, include more than 2500 kind of wild plant, these plants Every day all discharges oxygen and substantial amounts of trace element in air so that the air on 60,000 stockaded villages reaches The standard of natural oxygen-bar.And Herba Dendrobii is exactly by the moisture in root absorption air and oxygen Grow, therefore, after absorbing the air of 60,000 stockaded villages so high-quality so that Herba Dendrobii in Herba Dendrobii The composition of polysaccharide and alkaloid is the most ecological and naturalization;In addition, spring water here Ph value between 6.6 to 7.0, the beneficially growth of Herba Dendrobii.
Rice dry measure used in former times is typically grown between height above sea level 100-3000 rice height, or rock upper in tree of often growing nonparasitically upon another plant Shi Shang, likes warm, moistening and half cloudy environment, can not resist cold.Rice dry measure used in former times natural propagation ability low, Poor growth.And at present during rice dry measure used in former times biological engineering, percentage of seedgermination is often less than 20 %, the survival rate after transplanting is also less than 60%.This greatly limits the cultivation of meter dry measure used in former times.
It is an object of the invention to, complex operation many for the step faced in rice dry measure used in former times tissue culture propagation, The problem that cost is high, studies and improves, and proposes the method for quickly breeding of a kind of meter of dry measure used in former times breeding.
Summary of the invention
For above-mentioned deficiency present in prior art, the technical problem to be solved is The breeding method of a kind of meter of dry measure used in former times is provided.
The present invention seeks to be achieved through the following technical solutions:
The breeding method of a kind of meter of dry measure used in former times, comprises the following steps:
(1) by the rice dry measure used in former times seed collection after maturation, dry, be put in cool place, be dried ventilation Preserve;
(2) at the top covering activity shade net of sowing booth, the surrounding of sowing booth covers Thin film, seed bed overhead height is 35-55cm;
(3) being layered on and do on the seed bed of backing by cultivating base with linoleum cloth, obtaining thickness is The plantation layer of 5-15cm;
(4) rice dry measure used in former times seed is sowed at plantation layer on plant, then water to plantation layer contain The water yield is 75-90wt%, planting conditions: illumination 10-15 hour every day, illuminance 1000-3000lx, temperature is 20-27 DEG C, and air humidity is 80-90%.
Preferably, the top covering activity shade net of the sowing booth in described step (2) Area accounts for the 70-80% of the sowing booth gross area, the area of the surrounding cover film of sowing booth Account for the 20-30% of the sowing booth gross area.
Preferably, the cultivation base in described step (3), the raw material of following weight parts prepare Form: peanut residue 15-25 part, rapeseed meal 5-15 part, wood bran 65-75 part, magnesium sulfate 0.5-3 Part, double superhosphate 1-5 part, potassium nitrate 1-5 part, boric acid 0.1-0.5 part, enzyme 0.2-2 part, Enzyme live keeping agent 0.5-5 part.
Preferably, described enzyme is in subtilisin, urease, papain one Kind or multiple mixture.
It is highly preferred that described enzyme is mixed by subtilisin, urease, papain Conjunction forms, and described subtilisin, urease, the mass ratio of papain are (1-3): (1-3): (1-3).
Preferably, described enzyme live keeping agent is a kind of or many in fructose, dextrinose, cottonseed sugar The mixture planted.
It is highly preferred that described enzyme live keeping agent is mixed by fructose, dextrinose, cottonseed sugar, Described fructose, dextrinose, the mass ratio of cottonseed sugar are (1-3): (1-3): (1-3).
Preferably, the employing atomized water spray that waters in described step (4).
The breeding method of rice dry measure used in former times of the present invention, uses the seed of rice dry measure used in former times and cultivation base to carry out the numerous of seed Growing cultivation, seed cultivates, by absorbing, the nutriment that base provides, it is possible to fast-germination, grows up to and has Stem and the seedling of leaf.The present invention improves the germination percentage of seed, solves the plantation in field simultaneously Environmental factors restricts, and as climate impact is relatively big, and pest and disease damage is serious and is difficult to the problem prevented and treated, Protect the breeding of meter dry measure used in former times.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described further, the following stated, is only to this The preferred embodiment of invention, not does the restriction of other forms to the present invention, any familiar Professional and technical personnel is changed to become on an equal basis possibly also with the technology contents of the disclosure above The Equivalent embodiments changed.Every without departing from the present invention program content, the technology according to the present invention is real Any simple modification that confrontation following example are done or equivalent variations, all fall within the guarantor of the present invention In the range of protecting.
Each raw material introduction in embodiment:
Magnesium sulfate, No. CAS: 7487-88-9.
Double superhosphate, No. CAS: 65996-95-4.
Potassium nitrate, No. CAS: 7757-79-1.
Boric acid, No. CAS: 10043-35-3
Subtilisin, CAS:9014-01-1, the graceful bio tech ltd of upper Hypon There is provided, enzyme 200U/mg alive.
Urease, No. CAS: 9002-13-5, the graceful bio tech ltd of upper Hypon provides, Enzyme 200U/mg alive.
Papain, No. CAS: 9001-73-4, Zhengzhou Kang Yuan chemical products company limited There is provided, enzyme 200U/mg alive.
Fructose, No. CAS: 7660-25-5.
Dextrinose, No. CAS: 499-40-1.
Cottonseed sugar, No. CAS: 512-69-6.
Wood flour, phosphorus content 48.47%, nitrogen content 0.3430%.
Rapeseed meal, phosphorus content 22.36%, nitrogen content 0.3422%.Use precompressed extract technology By-product during Semen Brassicae campestris is extracted oil, preferably 200 type rapeseed meal.
Peanut residue, phosphorus content 43.10%, nitrogen content 0.4788%.
Embodiment 1
The breeding method of rice dry measure used in former times, comprises the following steps:
(1) the rice dry measure used in former times seed after maturation (is plucked from Huoshan County 60,000 stockaded village's height above sea level 644 meters Rice dry measure used in former times planting base) gather, dry, it is put in cool place, is dried ventilation preservation, prevent mouldy Rotten;
(2) at the top covering activity shade net of sowing booth, the surrounding of sowing booth covers Thin film, wherein, the area of the top covering activity shade net of sowing booth accounts for the sowing total face of booth Long-pending 75%, the area of the surrounding cover film of sowing booth accounts for the sowing booth gross area 25%;Seed bed overhead height is 40cm;
(3) being layered on and do on the seed bed of backing by cultivating base with linoleum cloth, the thickness of paving is 8cm, does plantation layer;Obtain the plantation layer that thickness is 8cm;
(4) rice dry measure used in former times seed is sowed on plantation layer and plants, every square metre of plantation layer sowing 100, dry measure used in former times seed of rice, then watering to plantation layer water content with nebulizer is 80wt%, plants Period in time water holding plantation layer water content be 80wt%, illumination every day 12 hours, illumination Degree 2000lx, controlling temperature is 22 DEG C, and air humidity is 85%;Rice is obtained after planting 6 weeks Dry measure used in former times seedling.
Cultivation based raw material (weight portion) in described step (3): peanut residue 20 parts, Semen Allii Tuberosi The dregs of rice 10 parts, wood bran 70 parts, 1 part of magnesium sulfate, double superhosphate 2.5 parts, potassium nitrate 3.5 Part, boric acid 0.2 part, subtilisin 0.3 part, urease 0.3 part, Papain Enzyme 0.3 part, 0.4 part of fructose, dextrinose 0.4 part, cottonseed sugar 0.4 part.
Prepared by cultivation base: peanut residue, rapeseed meal, wood powder are broken to 10 mesh respectively, then mix Close uniformly, be then heated to 90 DEG C of holdings and carry out high-temperature sterilization in 5 minutes, obtain compound; Magnesium sulfate, double superhosphate, potassium nitrate, boric acid are added mix homogeneously in compound, then will Fructose, dextrinose, cottonseed sugar add mix homogeneously in compound, finally by bacillus subtilis egg White enzyme, urease, papain add mix homogeneously in compound, obtain cultivating base.
Embodiment 2
Substantially the same manner as Example 1, differ only in:
Cultivation based raw material (weight portion) in described step (3): peanut residue 20 parts, Semen Allii Tuberosi The dregs of rice 10 parts, wood bran 70 parts, 1 part of magnesium sulfate, double superhosphate 2.5 parts, potassium nitrate 3.5 Part, boric acid 0.2 part, subtilisin 0.45 part, urease 0.45 part, fructose 0.4 Part, dextrinose 0.4 part, cottonseed sugar 0.4 part.
Prepared by cultivation base: peanut residue, rapeseed meal, wood powder are broken to 10 mesh respectively, then mix Close uniformly, be then heated to 90 DEG C of holdings and carry out high-temperature sterilization in 5 minutes, obtain compound; Magnesium sulfate, double superhosphate, potassium nitrate, boric acid are added mix homogeneously in compound, then will Fructose, dextrinose, cottonseed sugar add mix homogeneously in compound, finally by bacillus subtilis egg White enzyme, urease add mix homogeneously in compound, obtain cultivating base.
Embodiment 3
Substantially the same manner as Example 1, differ only in:
Cultivation based raw material (weight portion) in described step (3): peanut residue 20 parts, Semen Allii Tuberosi The dregs of rice 10 parts, wood bran 70 parts, 1 part of magnesium sulfate, double superhosphate 2.5 parts, potassium nitrate 3.5 Part, boric acid 0.2 part, subtilisin 0.45 part, papain 0.45 part, really Sugar 0.4 part, dextrinose 0.4 part, cottonseed sugar 0.4 part.
Prepared by cultivation base: peanut residue, rapeseed meal, wood powder are broken to 10 mesh respectively, then mix Close uniformly, be then heated to 90 DEG C of holdings and carry out high-temperature sterilization in 5 minutes, obtain compound; Magnesium sulfate, double superhosphate, potassium nitrate, boric acid are added mix homogeneously in compound, then will Fructose, dextrinose, cottonseed sugar add mix homogeneously in compound, finally by bacillus subtilis egg White enzyme, papain add mix homogeneously in compound, obtain cultivating base.
Embodiment 4
Substantially the same manner as Example 1, differ only in:
Cultivation based raw material (weight portion) in described step (3): peanut residue 20 parts, Semen Allii Tuberosi The dregs of rice 10 parts, wood bran 70 parts, 1 part of magnesium sulfate, double superhosphate 2.5 parts, potassium nitrate 3.5 Part, boric acid 0.2 part, urease 0.45 part, papain 0.45 part, 0.4 part of fructose, Dextrinose 0.4 part, cottonseed sugar 0.4 part.
Prepared by cultivation base: peanut residue, rapeseed meal, wood powder are broken to 10 mesh respectively, then mix Close uniformly, be then heated to 90 DEG C of holdings and carry out high-temperature sterilization in 5 minutes, obtain compound; Magnesium sulfate, double superhosphate, potassium nitrate, boric acid are added mix homogeneously in compound, then will Fructose, dextrinose, cottonseed sugar add mix homogeneously in compound, finally by urease, wood Melon protease adds mix homogeneously in compound, obtains cultivating base.
Embodiment 5
Substantially the same manner as Example 1, differ only in:
Cultivation based raw material (weight portion) in described step (3): peanut residue 20 parts, Semen Allii Tuberosi The dregs of rice 10 parts, wood bran 70 parts, 1 part of magnesium sulfate, double superhosphate 2.5 parts, potassium nitrate 3.5 Part, boric acid 0.2 part, subtilisin 0.3 part, urease 0.3 part, Papain Enzyme 0.3 part, 0.6 part of fructose, dextrinose 0.6 part.
Prepared by cultivation base: peanut residue, rapeseed meal, wood powder are broken to 10 mesh respectively, then mix Close uniformly, be then heated to 90 DEG C of holdings and carry out high-temperature sterilization in 5 minutes, obtain compound; Magnesium sulfate, double superhosphate, potassium nitrate, boric acid are added mix homogeneously in compound, then will Fructose, dextrinose add mix homogeneously in compound, finally by subtilisin, urine Element enzyme, papain add mix homogeneously in compound, obtain cultivating base.
Embodiment 6
Substantially the same manner as Example 1, differ only in:
Cultivation based raw material (weight portion) in described step (3): peanut residue 20 parts, Semen Allii Tuberosi The dregs of rice 10 parts, wood bran 70 parts, 1 part of magnesium sulfate, double superhosphate 2.5 parts, potassium nitrate 3.5 Part, boric acid 0.2 part, subtilisin 0.3 part, urease 0.3 part, Papain Enzyme 0.3 part, 0.6 part of fructose, cottonseed sugar 0.6 part.
Prepared by cultivation base: peanut residue, rapeseed meal, wood powder are broken to 10 mesh respectively, then mix Close uniformly, be then heated to 90 DEG C of holdings and carry out high-temperature sterilization in 5 minutes, obtain compound; Magnesium sulfate, double superhosphate, potassium nitrate, boric acid are added mix homogeneously in compound, then will Fructose, cottonseed sugar add mix homogeneously in compound, finally by subtilisin, carbamide Enzyme, papain add mix homogeneously in compound, obtain cultivating base.
Embodiment 7
Substantially the same manner as Example 1, differ only in:
Cultivation based raw material (weight portion) in described step (3): peanut residue 20 parts, Semen Allii Tuberosi The dregs of rice 10 parts, wood bran 70 parts, 1 part of magnesium sulfate, double superhosphate 2.5 parts, potassium nitrate 3.5 Part, boric acid 0.2 part, subtilisin 0.3 part, urease 0.3 part, Papain Enzyme 0.3 part, dextrinose 0.6 part, cottonseed sugar 0.6 part.
Prepared by cultivation base: peanut residue, rapeseed meal, wood powder are broken to 10 mesh respectively, then mix Close uniformly, be then heated to 90 DEG C of holdings and carry out high-temperature sterilization in 5 minutes, obtain compound; Magnesium sulfate, double superhosphate, potassium nitrate, boric acid are added mix homogeneously in compound, then will Dextrinose, cottonseed sugar add mix homogeneously in compound, finally by subtilisin, Urease, papain add mix homogeneously in compound, obtain cultivating base.
Test case 1
Adding up the percentage of seedgermination of embodiment 1-7, concrete outcome is shown in Table 1.
Table 1: germination percentage result table
Germination percentage (%)
Embodiment 1 75
Embodiment 2 54
Embodiment 3 62
Embodiment 4 57
Embodiment 5 56
Embodiment 6 51
Embodiment 7 55
Comparing embodiment 1 and embodiment 2-4, embodiment 1 (subtilisin, carbamide Enzyme, papain are compounding) germination percentage apparently higher than embodiment 2-4 (subtilisin, In urease, papain, arbitrarily the two is compounding);Comparing embodiment 1 and embodiment 5-7, Embodiment 1 (fructose, dextrinose, cottonseed sugar are compounding) germination percentage is apparently higher than embodiment 5-7 (in fructose, dextrinose, cottonseed sugar, arbitrarily the two is compounding).
Test case 2
To the rice dry measure used in former times seedling replanting of embodiment 1-7 to big field, add up survival rate, concrete outcome It is shown in Table 2.
Table 2: survival results table
Survival rate (%)
Embodiment 1 100
Embodiment 2 96
Embodiment 3 93
Embodiment 4 95
Embodiment 5 92
Embodiment 6 93
Embodiment 7 91
Comparing embodiment 1 and embodiment 2-4, embodiment 1 (subtilisin, carbamide Enzyme, papain are compounding) survival rate apparently higher than embodiment 2-4 (subtilisin, In urease, papain, arbitrarily the two is compounding);Comparing embodiment 1 and embodiment 5-7, Embodiment 1 (fructose, dextrinose, cottonseed sugar are compounding) survival rate is apparently higher than embodiment 5-7 (in fructose, dextrinose, cottonseed sugar, arbitrarily the two is compounding).
Test case 3
The rice dry measure used in former times seedling of embodiment 1-7 is carried out nutritional labeling and the test of plant thickness, specifically ties Fruit is shown in Table 3.
Table 3: nutritional labeling and plant thickness test result table
Protein content, % Crude polysaccharides content, % Plant thickness, cm
Embodiment 1 29.7 58.6 0.51
Embodiment 2 27.6 56.2 0.43
Embodiment 3 27.1 56.6 0.46
Embodiment 4 27.4 56.5 0.44
Embodiment 5 26.5 56.1 0.45
Embodiment 6 27.1 55.7 0.41
Embodiment 7 26.4 55.4 0.43
Comparing embodiment 1 and embodiment 2-4, embodiment 1 (subtilisin, carbamide Enzyme, papain are compounding) to be substantially better than embodiment 2-4 (withered for nutritional labeling and plant thickness In grass Bacillus protease, urease, papain, arbitrarily the two is compounding);Comparing embodiment 1 with embodiment 5-7, embodiment 1 (fructose, dextrinose, cottonseed sugar are compounding) nutritional labeling It is substantially better than embodiment 5-7 (in fructose, dextrinose, cottonseed sugar any two with plant thickness Person compounds).

Claims (8)

1. a breeding method for rice dry measure used in former times, comprises the following steps:
(1) by the rice dry measure used in former times seed collection after maturation, dry, be put in cool place, be dried ventilation Preserve;
(2) at the top covering activity shade net of sowing booth, the surrounding of sowing booth covers Thin film, seed bed overhead height is 35-55cm;
(3) being layered on and do on the seed bed of backing by cultivating base with linoleum cloth, obtaining thickness is The plantation layer of 5-15cm;
(4) rice dry measure used in former times seed is sowed at plantation layer on plant, then water to plantation layer contain The water yield is 75-90wt%, planting conditions: illumination 10-15 hour every day, illuminance 1000-3000lx, temperature is 20-27 DEG C, and air humidity is 80-90%.
The breeding method of the most as claimed in claim 1 meter of dry measure used in former times, it is characterised in that:
Cultivation base in described step (3), is prepared from by the raw material of following weight parts: flower Raw bran 15-25 part, rapeseed meal 5-15 part, wood bran 65-75 part, magnesium sulfate 0.5-3 part, weight Calcium superphosphate 1-5 part, potassium nitrate 1-5 part, boric acid 0.1-0.5 part, enzyme 0.2-2 part, enzyme are protected Agent 0.5-5 part alive.
The breeding method of the most as claimed in claim 2 meter of dry measure used in former times, it is characterised in that: described Enzyme is the mixture of one or more in subtilisin, urease, papain.
The breeding method of the most as claimed in claim 3 meter of dry measure used in former times, it is characterised in that: described Enzyme is mixed by subtilisin, urease, papain, described bacillus subtilis Protease, urease, the mass ratio of papain are (1-3): (1-3): (1-3).
5. the breeding method of the rice dry measure used in former times as according to any one of claim 1-4, its feature exists In: described enzyme live keeping agent is the mixing of one or more in fructose, dextrinose, cottonseed sugar Thing.
The breeding method of the most as claimed in claim 5 meter of dry measure used in former times, it is characterised in that: described Enzyme live keeping agent is mixed by fructose, dextrinose, cottonseed sugar, described fructose, dextrinose, The mass ratio of cottonseed sugar is (1-3): (1-3): (1-3).
7. the breeding method of the rice dry measure used in former times as according to any one of claim 1-6, its feature exists In: the area of the top covering activity shade net of the sowing booth in described step (2) accounts for be broadcast Planting the 70-80% of the booth gross area, it is big that the area of the surrounding cover film of sowing booth accounts for sowing The 20-30% of the canopy gross area.
8. the breeding method of the rice dry measure used in former times as according to any one of claim 1-7, its feature exists In: the employing atomized water spray that waters in described step (4).
CN201610305097.6A 2016-05-06 2016-05-06 Dendrobium huoshanense C.Z.Tang et S.J.Cheng breeding method Pending CN105941111A (en)

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Application publication date: 20160921