CN1088980C - High effect microorganism seed growth accelerating agent - Google Patents

High effect microorganism seed growth accelerating agent Download PDF

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Publication number
CN1088980C
CN1088980C CN 96120571 CN96120571A CN1088980C CN 1088980 C CN1088980 C CN 1088980C CN 96120571 CN96120571 CN 96120571 CN 96120571 A CN96120571 A CN 96120571A CN 1088980 C CN1088980 C CN 1088980C
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bacterium
seed growth
accelerating agent
growth accelerating
microorganism seed
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CN1158217A (en
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刘文治
宋卉
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Abstract

The present invention discloses a high-efficiency microorganism seed growth promotion agent product which uses microorganisms generating proteinase, amylase, hemicellulase, pectic enzyme and cellulose enzyme as a main bacterium group and uses saccharomycete, phosphorus bacteria, silicate bacteria, bacillus acidophilus and streptococcus thermophilus as an auxiliary bacterium group, and the two groups of bacterium groups are formed into an ecological bacterium group. The main bacterium group quickens the nutrition conversion and consumption of seeds, so seedlings of the seeds can be early emerged, and the roots of the seeds can be early fixed; then quick-acting nutrients in soil can be increased by the conversion of the saccharomycete, the phosphorus bacteria and the silicate bacteria, and the bacillus acidophilus and the streptococcus thermophilus can suppress diseas bacteria and have the health care action on the seed seedlings. Therefore, the plant growth is quickened, plants can be ripe head of time, and the yield and the income are increased.

Description

A kind of microorganism seed growth accelerating agent
The invention discloses a kind of microorganism seed growth accelerating agent product.
The invention belongs to microorganism or enzyme, its combination field.
The domestic microbial manure that also claims of traditional microbial inoculant claims bio-fertilizer abroad.The current bacterial classification that is adopted is azotobacter, rhizobium, phosphobacteria, silicate bacteria both at home and abroad.Get its single culture or the mixed bacteria more than two kinds, with its bacterium liquid attached on the peat composed of rotten mosses or the vermiculite.Make powdery Inoculant or granular pattern Inoculant, also have direct with bacterium liquid.Above Inoculant its mainly to act on be to increase rhizospheric microorganism quantity.With its competitive advantage, repel the wild rhizospheric microorganism of soil (comprising the disease bacterium).Can play and alleviate disease, gaseous nitrogen is fixedly changed into inorganic nitrogen.Invalid phosphorus potassium in the soil can also be changed into available phosphorus potassium, increase the available nutrient in the soil.This Inoculant needs can work behind seed sprouting, normal root.Seed is emerged from sprouting needs to consume earlier the nutrition of seed self.Treating that the seed idiotrophic transforms runs out, and just emerges, normal root, absorbs nutrition in the environment.The process that the seed idiotrophic transform to consume is very fragile, is subject to the harmful effect of surrounding environment and reduces emergence rate.Shorten this time, make it early to emerge, normal root early, will produce significant impact throughout one's life crop.The tradition Inoculant can't resolve this problem.
In recent years, develop a kind of seed coating medicine both at home and abroad.Coating material major function composition is inorganic fertilizer, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer or adds trace element.Also there is chemical fertilizer to add agricultural chemicals, as thimet etc.The purpose that adds agricultural chemicals is to solve soybean continuous cropping problem, kills fungus disease bacterium and nematode in the soybean continuous cropping soil.The inorganic nutrients seed coating medicine mainly is to increase near the nutritional amt root, make the seedling normal root after, be easy to absorb nutrition, accelerate growth.But traditional nutrition type seed coating medicine also needs seed germination to emerge to work behind the normal root, still can't resolve seed and accelerates the problem that idiotrophic transforms and consumes.
The objective of the invention is to have broken through above-mentioned two kinds of traditional modes.With can decomposition of cellulose, two kinds of bacilluss of hemicellulose and pectin, a kind ofly produce loosening kind of the skin of enzyme that the yellowish fiber monad is produced, make moisture by the seed fast Absorption.Then, produce protease and diastatic two kinds of bacillus subtilises the starch in the seed is resolved into glucose, the protein in the seed is resolved into amino acid, the nutrition of seed parent transforms fast more, and it is fast more that seedling grows, fixed just fast more of root.The conversion of nutrition needs enough moisture.Water-absorbing resins in the product of the present invention can will be preserved enough moisture in the soil, saccharomycete can increase organic nitrogen nutrition in the soil.Phosphobacteria and silicate bacteria can change into available phosphorus, potassium with the rock phosphate of product of the present invention, invalid phosphorus, the potassium in the potassium feldspar.Simultaneously, also invalid phosphorus, potassium in the soil can be changed into rapid available phosphorus, potassium.Make root system development good.Lactobacillus acidophilus, streptococcus thermophilus can suppress the disease bacterium.
Another object of the present invention is in order to overcome the weak point of traditional Inoculant and seed coating medicine, to make the nutrition of seed body transform and consume quickening, improve seeding ratio, early go out mu, having gone out seedling.Premature ripening reaches stable and high yields.
Content of the present invention is that the microorganism that is used to produce amylase, protease, hemicellulase, pectase, cellulase is main flora, with the microorganism that can increase organic nitrogen nutrition, rapid available phosphorus, potassium nutrition and can suppress disease for paying a flora, both have constituted one group of ecological flora, do Inoculant with this, do carrier with a kind of organic matter and ore class; Water-absorbing resins with modified starch, modification humus graft copolymer class is made water-loss reducer; Make activator with trace element; Cook nutritional agents with organic carbide, nitride; Make adhesive with organic high molecular polymer; With phosphorous, contain the potassium breeze and do reinforcing agent; Above composition constitutes the high effect microorganism seed growth accelerating agent product.One group of ecological flora, its main flora is the bacterium that can produce cellulase, for producing the yellowish fiber monad.Can produce the bacterium of hemicellulase, pectase, be bacillus.Their effect is to make kind of skin loosening, makes moisture infiltrate seed inside fast, increases the activation capacity of enzyme.Can produce protease, diastatic two kinds of bacterium bacillus subtilises help seed with its protein resolve into amino acid, starch resolves into glucose, consumes the nutrition of seed itself fast.Accelerate the formation of seedling, make it normal root early, and improve emergence rate.
This has just remedied traditional Inoculant and seed coating medicine, only in the shortcoming of emerging, just working behind the normal root.Lactobacillus acidophilus, streptococcus thermophilus, saccharomycete, phosphobacteria, the silicate bacterium that flora comprises the bacterium class of paying of forming ecological flora; Pay flora in the ecological flora, what wherein can increase organic nitrogen nutrition is the candida tropicalis of budding fungus; What can increase rapid available phosphorus nutrition is the bacillus megaterium of bacterium class, and what can increase available potassium nutrition is the bacillusmusilaginosiengineering of bacterium class, and that can suppress the disease bacterium is the lactobacillus acidophilus and the streptococcus thermophilus of bacterium class.Pay flora and mainly be and emerging, working behind the normal root, yeast class candida tropicalis wherein can be that crop provides organic nitrogen nutrition with the form of single cell protein.Stronger than traditional azotobacter vitality, the nitrogen nutrition that provides is many.Bacterium class bacillus megaterium can change into rapid available phosphorus with invalid phosphorus.The silicate bacterium of bacterium class also is bacillusmusilaginosiengineering, invalid potassium can be changed into available potassium.Saccharomycetic dead thalline, single cell protein can resolve into amino acid, polypeptide by protease, until ammonia, can provide quick-acting nitrogens for crops seedling stage, make root system absorb more nutrition.Cellulase, hemicellulase, pectase can resolve into water miscible organic carbon with the organic carbon in undesirable root in the soil and the dead thalline simultaneously, are made for the needs of thing to carbon nutrition.Main flora not only can play the effect that transforms nutrition in early stage.After can also emerging normal root in the later stage, play conversion nutrition and provide quick-acting nutritious effect for crop.The viable count of ecological flora bacterium liquid is no less than hundred million of 10-20 for every milliliter.Bacterium liquid dosage is the 10-40% of product weight.The viable count of the ecological flora of product is controlled at hundred million of every gram 1-5.Its target level of product quality is controlled by the microbial manure industry standard GB15063-94 that the Ministry of Agriculture 1994 issues.
The used bacterial classification of ecological flora is as follows in the product of the present invention:
Classification Chinese name The Latin literary fame Preserve number Preserve day
The bacterium class Bacillus subtilis (product protease) Bacillus?subticis ?CGMCC.NO0244 ?1995.11.18
The bacterium class Bacillus (product amylase) Bacukkys syseicis ?CGMCC.NO.0244 ?1995.11.18
The bacterium class Bacillus subtilis (product hemicellulose) Bacillus?subtilis ?CGMCC.NO.0280 ?1995.11.18
The bacterium class Bacillus subtilis (product pectase) Bacillus?sublilis ?CGMCC.NO.0280 ?1995.11.18
The bacterium class Produce yellowish fiber monad (cellulase-producing) Cellulomonas flaigena ?CGMCC.NO.0280 ?1995.11.18
The yeast class Candida tropicalis (producing single bacteria albumen) Candida tropicalis ?CGMCC.NO.0280 ?1995.11.18
The bacterium class Bacillus megaterium (phosphorus decomposing) Bacillus megatherium ?CGMCC.NO.0280 ?1995.11.18
The bacterium class Bacillusmusilaginosiengineering (potassium decomposing) Bacillus mucilagimosus ?CGMCC.NO.0280 ?1995.11.18
The bacterium class Lactobacillus acidophilus (pressing down sick) Laclobacillus acidophilus ?CGMCC.NO.0280 ?1995.11.18
The bacterium class Streptococcus thermophilus (pressing down sick) Ser.thermophilns ?CGMCC.NO.0280 ?1995.11.18
The carrier of product of the present invention is exactly the place that ecological flora is lived away from home.We adopt peat composed of rotten mosses powder, vermiculite power fineness is the 100-200 order, one or both mixing.Its ratio is the 50-80% of product weight.Survive better for guaranteeing product microbial bacteria group energy of the present invention, seed can absorb enough moisture in soil, add water-loss reducer in the product, its composition is the water-absorbing resins of modified starch graft copolymer modification humus graft copolymer class, its mixing a kind of or more than two kinds.Its dosage is the 0.5-5 ‰ of product weight.
In the product of the present invention, need to make the activator of enzyme system of trace element and yttrium.The activator of trace element comprises that the salt dosage of Fe, Mn, Cu, Zn, Mo, B is the 0.1-5 ‰ of product weight, and rare metal activator natural medical stone wherein has more than 30 kind of yttrium, and addition is the 2-5% of product weight.
Product of the present invention need be dressed seed or dressing when using.For bonding with seed better, adopt one or more mixing such as organic high molecular polymer such as modified starch adhesive, protein adhesive, polyvinyl alcohol, carboxymethyl cellulose, polyacrylate.Addition is the 2-10% (in butt) of product weight.
Reinforcing agent is done in one or both mixing of rock phosphate and feldspar in powder in the product of the present invention.Simultaneously provide phosphorus, potassium nutrition element for soil again.Fineness is the 100-200 order, and dosage is the 5-20% of product weight.
For making the ecological flora in the product of the present invention can increase reproduction speed, need to replenish organic carbide, nitride nutrition.Organic carbide is with glucose, nitride urea.The glucose dosage is the 4-8% of product weight, and urea is 1% of product weight.
In sum, product of the present invention is made of ecological flora, carrier, water-loss reducer, activator, adhesive, reinforcing agent.Its superiority is the shortcoming that remedies traditional Inoculant and seed coating medicine, promotes the nutrition of seed itself to transform and consume, and makes that seed is sprouted soon, emerged soon, normal root soon.
The good effect of using product of the present invention is to make crop do sth. in advance to emerge in 3-4 days, makes emergence rate improve 15%, premature ripening 5-7 days, on average increases production 15-20%.
Seed dressing, dressing all can when using.Consumption is 0.5 kilogram on every mu of ground.
Accompanying drawing is a process chart of the present invention, and is below in conjunction with drawings and Examples, as follows to the detailed description of the invention:
Accompanying drawings embodiment
Each bacterial classification is cultivated respectively, and enlarged culture is briefly explained with a cover process chart respectively.
Former bacterial classification 1 is activated 2, carries out solid inclined-plane enlarged culture 3, shakes bottle enlarged culture 4 then, then carries out seeding tank enlarged culture 5, carries out fermentation tank enlarged culture 6 at last, enters three mixed bacteria liquid basins at last.The 7-1 basin is equipped with and produces protease, diastatic bacillus subtilis, produces 4 kinds of bacterium liquid such as bacillus of hemicellulase, pectic substance enzyme.The 7-2 basin is equipped with two kinds of bacterium liquid such as phosphobacteria, silicate bacterium.The 7-3 basin is equipped with two kinds of bacterium liquid such as lactobacillus acidophilus, streptococcus thermophilus.Carrier 8-1 after the mixed bacteria liquid 7-1 of three jars, 7-2,7-3 pulverize with sterilization respectively, 8-2,8-3 mix and adhere to.Carry out the low temperature dewatering drying then respectively.9-1,9-2,9-3 bacillus baking temperature is 60-80 ℃, non-bacillus baking temperature is 30-60 ℃.Do not exceed to kill viable bacteria.Water content is controlled in 12%.Three kinds of carriers that carry disease germs behind the low temperature dewatering are through mixing 10, then with adhesive 11-1-(1), micro-11-1-(2), medical stone 11-1-(3), water-loss reducer 11-1-(4), nutritional agents 11-1-(5), reinforcing agent 11-1-(6) gives the 11-1 that mixes earlier, mix 11 at last, by after the quality inspection 12, carry out metering packing 13 again, be finished product warehouse-in 14 then.

Claims (9)

1, a kind of microorganism seed growth accelerating agent, it is characterized in that with producing diastatic bacillus, produce the bacillus subtilis of protease, produce the bacillus subtilis of hemicellulose, produce the bacillus subtilis of pectase, the product yellowish fiber monad of cellulase-producing is main thalline, produce the candida tropicalis of single cell protein with increasing organic nitrogen nutrition, what can increase rapid available phosphorus nutrition is the bacillus megaterium of bacterium class, what can increase available potassium nutrition is the bacillusmusilaginosiengineering of bacterium class, that can suppress the disease bacterium is the lactobacillus acidophilus and the streptococcus thermophilus of bacterium class, and silicate bacterium is for paying a flora, and both have constituted one group of ecological flora, do Inoculant with this, one or both mixture with oxalic acid or vermiculite is a carrier, uses modified starch, and the water-absorbing resins of modification humus graft copolymer class is made water-loss reducer; Make activator with trace element; Cook nutritional agents with organic carbide, nitride, make adhesive with organic high molecular polymer; With phosphorous, contain the potassium breeze and do reinforcing agent; Above composition constitutes the high effect microorganism seed growth accelerating agent product, and its ecological flora bacterium liquid dosage is the 10-40% of product weight, and the viable count of bacterium liquid is that every ml is no less than hundred million of 10-20.
2, by the described microorganism seed growth accelerating agent of claim 1, it is characterized in that carrier for being ground into 100-200 purpose grass carbon or vermiculite, the mixing of wherein one or both, its ratio is the 50-80% of product weight.
3, by the described microorganism seed growth accelerating agent of claim 1, it is characterized in that water-loss reducer is a modified starch graft copolymer class, modification humus graft copolymer class water-absorbing resins, dosage is the 0.5-5 ‰ of product weight.
4, by the described microorganism seed growth accelerating agent of claim 1, it is characterized in that activator is the salt of Fe, Mn, Cu, Zn, Mo, B, its addition is the 0.1-5 ‰ of product weight.
5, by the described microorganism seed growth accelerating agent of claim 1, it is characterized in that activator is the medical stone powder, its addition is the 2-5% of product weight.
6, by the described microorganism seed growth accelerating agent of claim 1, it is characterized in that organic high molecular polymer is one or two or more kinds mixing of modified starch adhesive, protein adhesive, polyvinyl alcohol, carboxymethyl cellulose, polyacrylate, dosage is that the 2-10% of product weight is in butt.
7, by the described microorganism seed growth accelerating agent of claim 1, that base is characterised in that is phosphorous, the potassium breeze is rock phosphate or one or both mixing of feldspar in powder, and fineness is the 100-200 order, and dosage is the 5-20% of product weight.
8, by the described microorganism seed growth accelerating agent of claim 1, it is characterized in that organic carbide, nitride are nutritional agents, organic carbide is a glucose, and nitride is a urea, and glucose is the 4-8% of product weight, and urea is 1%.
9, by the described microorganism seed growth accelerating agent of claim 1, it is characterized in that the viable count of ecological flora is controlled at hundred million of every gram 1-5.
CN 96120571 1996-11-19 1996-11-19 High effect microorganism seed growth accelerating agent Expired - Fee Related CN1088980C (en)

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CN1088980C true CN1088980C (en) 2002-08-14

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