CN1317953C - Antago nistic fungus capable of preventing and treating continuous crops blight and its microbial organic fertilizer - Google Patents
Antago nistic fungus capable of preventing and treating continuous crops blight and its microbial organic fertilizer Download PDFInfo
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- CN1317953C CN1317953C CNB2005101228980A CN200510122898A CN1317953C CN 1317953 C CN1317953 C CN 1317953C CN B2005101228980 A CNB2005101228980 A CN B2005101228980A CN 200510122898 A CN200510122898 A CN 200510122898A CN 1317953 C CN1317953 C CN 1317953C
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Fertilizers (AREA)
Abstract
The present invention relates to antagonistic bacteria and microorganism organic fertilizer prepared from the antagonistic bacteria for overcoming or eliminating the blight of continuous cropping crops, which belongs to the technology of agriculture intensive production. The present invention is separated to the antagonistic bacteria (bacillus) which have a conspicuous antagonistic effect on the original fungi of the blight. The antagonistic bacteria and the organic fertilizer are mixed to prepare the microorganism organic fertilizer. The fertilizer contains more than 1*10<8>/g antagonistic microorganism, and the content of the total nitrogen is from 4 to 5% (more than 90% is organic nitrogen). The total nitrogen, phosphorus and potassium macronutrient is from 6 to 10%, and organic matter content is from 30 to 35%. The test shows that after the microorganism organic fertilizer is applied into the soil, the antagonistic bacteria are rapidly propagated and form a superiority group in the soil. The prevention and cure rate of the blight of the soil having continuous cropping blight reaches more than 80%, and the fruit output of crops on continuous cropping soil on which the microorganism organic fertilizer is not applied nearly approaches to zero. After the microorganism organic fertilizer is applied to the soil which does not have a continuous copping disease, the blight of continuous cropping crops can be prevented to a great extent.
Description
One, technical field
A kind of antagonism bacterium and the microorganism organic fertilizer thereof that can prevent and kill off the succession crop fusarium wilt of the present invention belongs to the intensive agriculture production technology, is exclusively used in the fusarium wilt problem that overcomes and eliminate succession crop.
Two, technical background
The continuous cropping obstacle of crops such as melon and fruit, vegetables, cotton is the bottleneck problem of whole world intensification agricultural production, the industrialized agriculture soil of thousands of hectares is constantly thrown aside owing to continuous cropping obstacle, financial resources, man power and material that industrialized agriculture drops into have greatly been wasted, even if crops such as the cucumber of large tracts of land open country production, watermelon, tomato, soybean, green pepper, cotton also produce serious continuous cropping obstacle owing to plant year after year, greatly influenced the economic benefit that vast farmers tills the land.Peasants have to carry out disinfection with Celfume to these soil every 2-3, not only greatly strengthened peasant's production cost (800 yuan/mu), and, severe contamination soil environment, influence agricultural product security, the strict control of government or ban use of this type of soil disinfectant, but, it is very ignorant that vast farmerses are felt, appeals that sharply the government and the departments of science and technology can give directions to them, to save the incident that this runs down.The continuous cropping fusarium wilt is the most serious obstruction factor in the soil obstacle of succession crop, and it mainly causes by fungal pathogen bacterium (Fusarium oxysporum), just begins to occur when 2 batches of continuous croppings, and continuous cropping does not have receipts with regard to basic granules for 4 batches.Our result that studies for a long period of time shows that the key issue of succession crop fusarium wilt is not nutrient dificiency or imbalance, neither the soil secondary salinization problem, but the intoxicating problem of microflora in the soil and secondary substance thereof.
On the other hand, the feces of livestock and poultry behind crops straw on-site incineration, the large-scale cultivations such as paddy rice, wheat, corn, rape has been thrown aside etc. not only severe contamination everywhere environment, also greatly having wasted can be as the raw material of fertilizer and biological organic fertilizer product; A large amount of nutrient resource (C, N, P, K, S and trace element) runs off outside soil-plant system, has weakened the ability of China's agricultural sustainable development significantly.The nutritive element how will take away because of harvesting crops from soil is returned in the soil to greatest extent and goes, and unique approach is made the organic commercial fertilizer material with these solid organic castoffs exactly and is manured into soil.If these solid organic castoffs through the synthetic high-grade organic compost of hot fermentation, are used further to the carrier of function bacterium, made microorganism organic fertilizer definite functions will have good application prospects.
Three, summary of the invention
1. technical problem
The objective of the invention is to develop a kind of antagonism bacterium and the microorganism organic fertilizer thereof that can prevent and kill off the succession crop fusarium wilt, overcome the succession crop fusarium wilt or eliminate succession crop fusarium wilt efficient and reach more than 80%, make a large amount of industrialized agriculture soil and open country continuous cropping obstacle soil obtain biological restoration, guarantee agriculture the shaping up that intensify.
2. technical scheme
A kind of antagonism bacterium that can overcome or eliminate the succession crop fusarium wilt, this microorganism is that the Paenibacillus polymyxa of Gram-positive belongs to the bacterial strain KKW-021 of (Paenibacillus polymyxa), be deposited at Chinese microorganism strain on September 15th, 2005 and preserve administration committee common micro-organisms center, culture presevation number is CGMCC NO.1544, and main biological property is: Gram-positive (G
+), cell shape is shaft-like (＜1 μ m), forms gemma (end is given birth to and expanded), the amphimicrobian growth, (oxidizing glucose produces sour aerogenesis for the positive of V.P＜pH6.0), the methyl red test positive for catalase reacting positive, oxydase reaction feminine gender, V.P. reaction, oxidation wood sugar, L-arabinose, mannitol produce acid, can not utilize citrate, nitrate reductase enzyme positive, the starch hydrolysis positive, the gelatin hydrolysis feminine gender is decomposed the casein positive.
Above-mentioned antagonism bacterium is to separate to obtain from the plant that fallen ill and soil, is bacillus, and by Molecular Identification, its 16srDNA sequence is SEQ ID.NO.1.
With above-mentioned a kind of can overcome or eliminate in the microorganism organic fertilizer that succession crop fusarium wilt antagonism bacterium produced contain 1 * 10
8Above antagonistic microbe quantity, the total nitrogen content of individual/g is 4～5% (being organic nitrogen 90% or more), and total nitrogen phosphorus potassium nutrition is 6～10%, the content of organic matter is 30～35%.Its production method comprises:
1) selects the antagonism bacterium for use, carry out fermenting and producing, the condition of its fermenting and producing is: pH growth scope nature, 24～41 ℃ of cultivation temperature scopes, dissolved oxygen: the throughput scope is 180～300 rev/mins under 30～100% conditions, and liquid nutrient medium adds richness for PDA monose, fermentation period 27～31 hours, the fermentation middle and later periods forms gemma, and the antimicrobial fluid short of money of fermentation contains bacterium (gemma) amount 〉=1 * 10
10Individual/ml;
2) with dry weight basis with crops straw: feces of livestock and poultry: inorganic nitrogeneous fertilizer is with 30～40: 60～70: 1～3 mixes after fermentation, treats to keep when compost temperature rises to 60-70 ℃ 5-7 days; Kept 5-7 days during compost temperature to 60 ℃, every 2-3 days turning once; Add 0.5～1% inorganic nitrogen during above-mentioned each turning again; Compost temperature began to lower the temperature 3～5 days from 50-60 ℃, stopped immediately, obtained the carrier organism organic materials of antagonism bacterium;
3) add antimicrobial fluid 10-50L short of money in the SOLID ORGANIC material per ton and mix, make and contain antagonism bacterium (gemma) quantity and reach 1 * 10
8The microorganism organic fertilizer that individual/g is above.
Antagonism bacterium fermentation optimum growth temperature is 37.5 ℃ in its production method, and the carbohydrate of enriched medium refers to glucose, sucrose.The used inorganic nitrogeneous fertilizer of biosynthesis organic nitrogen is ammonium chloride or ammonium sulfate.Used crops straw is paddy rice and the wheat stalk that is crushed to 1～5cm.The nitrogen of the carrier organism organic materials (organic compost) of used antagonism bacterium is organic nitrogen more than 90%.
3. beneficial effect
A kind of microorganism organic fertilizer and production method thereof that can overcome or eliminate the succession crop fusarium wilt of the present invention, utilize carbon source abundant in fermentative microorganism in the feces of livestock and poultry and the crops straw and small amounts of inorganic nitrogen to be fermented at short notice and contain rich soluble carbon and the high organic materials of organic nitrogen content, make microorganism organic fertilizer again with after antimicrobial fluid short of money is mixed, its product is compared with product in the market has following advantage:
1) contain the efficient bacterial strain (bacillus) that suppresses the growth of fusarium wilt fungal pathogens in the fertilizer product, it suppresses the effect highly significant, and the content of efficient bacterial strain reaches 1 * 10 in the fertilizer
8Individual/more than the g, energy formation advantage group after being manured into soil, and under in soil, surviving.
2) contain rich soluble carbon and organic nitrogen in the fertilizer product, can provide very superior growing environment, greatly improved the ability that function is grown and brought into play to external function bacterium deciding in soil to function bacterium.
3) composting process has added high temperature and middle temperature cellulose-decomposing bacteria, the compost fermentation time is controlled at (spring, summer, autumn) and 20～25 days (winter) about 15 days, simultaneously, control makes the decomposition of crops straw be in mid-term stage, can continue immediately to decompose after being manured into soil, thereby make a large amount of breedings of beneficial microbe fauna (being mainly bacterium) in the soil.
4) product organic phosphor of the present invention and organic nitrogen content are high especially, all reach 4%.One of this products material is an animal dung, and total phosphorus content is very high, and biological effectiveness is high especially, need not to add any phosphatization fertilizer that contains in making the microorganism organic fertilizer product, uses to make behind this product crop spend phosphorus in seedling stage plain sensitive periods smoothly.By contrast, it is relatively poor to contain the phosphatization fertilizer artifact validity that is manured into soil; And organic nitrogen can reach more than 4% in this product, has guaranteed the continual and steady supply of fertilizer nitrogen, make plant plant growth and anti-adversity ability strengthens greatly.
5) this production raw material sources extensively enrich, cost of material is very low, production technology is easy, the fixed assets less investment, and the power consumption water consumption is few in the production process, therefore the product price is low and with strong points, all costs of 1 ton of microorganism organic fertilizer of the every production of enterprise are no more than 600 yuan, and to be dedicated as the bio-organic fertilizer price very high and resemble this special efficacy, generally at 1500 yuan/ton, and very in short supply in the market, so this product has powerful society, environment and economic vaibility.
6) result of the test shows, after using this product on the soil of succession crops such as watermelon, cucumber, tomato, can significantly eliminate its continuous cropping fusarium wilt, elimination factor reaches more than 80%, do not use promptly that this fertilizer handles, the fruit yield of crop is not have 80% of continuous cropping obstacle soil almost near zero and use the crop and fruit output that this fertilizer handles on the continuous cropping soil, the continuous soil of using this fertilizer in 3 years, the continuous cropping obstacle elimination factor is higher.
Four, embodiment
The acquisition of antagonistic strain: gather the plant and the surrounding soil that continuous cropping obstacle have taken place and the fusarium wilt illness occurred, sample low temperature is preserved, adopt MGA2.5+PCNB fusarium wilt selective medium to obtain pathogenic strains, utilize NA improved culture medium, PDA improved culture medium and LB improved culture medium utilization face-off method to carry out antagonistic effect respectively, the primary dcreening operation antagonistic strain, sieve efficient antagonistic strain again by pot experiment, plant bacterial classification freeze-drying-20 ℃ preservation definite the genus through Physiology and biochemistry and 16srDNA Molecular Identification.
The production amplification culture of antagonistic strain: utilize the bacterial classification enriched medium to carry out the high density enlarged culture and obtain high gravity fermentation liquid (bacteria containing amount 〉=1 * 10
10Individual/ml), fermentation condition: medium PDA adds richness (add rich Main Ingredients and Appearance starch, wheat bran, sucrose etc. are arranged), 37.5 ℃ of cultivation temperature, pH7.0-7.4, dissolved oxygen early stage 100%-80%, mid-term, 80%-40%, later stage 60%-40%, last 100% were put a jar condition: living bacteria count 〉=1 * 10
10Individual/ml, gemma content 〉=95%.
With crops straw: feces of livestock and poultry: inorganic nitrogen mixes after fermentation by 30～40: 60～70: 1～3 (with dry weight basis), treat to keep when compost temperature rises to 60～70 ℃ 5～7 days (turning every day once), treat to keep when compost temperature rises to 60 ℃ of left and right sides 5～7 days (every 2-3 days turning once) again, 0.5～1% inorganic nitrogen is once added in above-mentioned every turning again, until compost temperature since 50～60 ℃ of coolings, keep again and stopped immediately in 3～5 days, air-dry stand-by.
The high-quality organic materials that the antimicrobial fluid short of money of fermenting and producing (having formed gemma) joins after the fermentation is mixed and made into microorganism organic fertilizer, add antimicrobial fluid 10-50L short of money in the SOLID ORGANIC material per ton, make antagonism bacterium (gemma) quantity in the microorganism organic fertilizer reach 1 * 10
8Individual/more than the g, packing is deposited in cool place, dry place.
Embodiment: with crops straw: feces of livestock and poultry: inorganic nitrogen mixes after fermentation by 30: 68: 2 (with dry weight basis), treat to keep when compost temperature rises to 60～70 ℃ 5～7 days (turning every day once), keep when treating compost temperature to the 60 ℃ left and right sides again 5～7 days (every 2-3 days turning once), 0.5% inorganic nitrogen is once added in above-mentioned every turning again, until compost temperature since 50～60 ℃ of coolings, keep again and stopped immediately in 3～5 days, air-dry stand-by.The antimicrobial fluid short of money (having formed gemma) of fermenting and producing joined in the high-quality organic materials after the fermentation be mixed and made into microorganism organic fertilizer, the main proterties such as the table 1 of its product.
Table 1 some characteristic of bio-organic fertilizer product of the present invention
| Total organic matter (%) | Soluble carbon (%) | Total organic nitrogen (%) | Total organic phosphorus (%) | Full potassium (%) | PH (flooding) | Antagonism bacterium viable bacteria (gemma) number (/g) |
| 30.2 | 4.9 | 4.12 | 4.36 | 1.47 | 6.8 | 1×10 8Individual |
Table 1 shows that organic (total organic carbon * 1.7) content is more than 30% in the product of producing by the technology of the present invention and production technology, and containing very at high proportion, the soluble carbon of (4.9%) (in carbon is, if then higher in organic matter), this is antagonism function bacterium and the prolific material base of indigenous beneficial microbe (bacterium) after this product is manured into soil; Also having a characteristic in this product is exactly that organic nitrogen and organic phosphor content are high especially, reach 4.12% and 4.36% respectively, not only significantly promote the biologically active of edaphon after being manured into soil, and improve the property supplied with of soil nitrogen phosphorus nutrient greatly, make in the crop growth phase nutrition in shape; The antagonism bacterium viable count of antagonism fusarium wilt reaches 1 * 10 in the product
8Individual/g, and be the gemma state more than 95%, the product holding time is longer.Above characteristic has proved absolutely the feasibility of the extraordinary microorganism organic fertilizer that the technology of the present invention and production technology are made.
The extraordinary bio-organic fertilizer that the present invention developed is to the yield increasing effect highly significant (seeing Table 2) of crop on the continuous cropping soil, if on the soil that the succession crop fusarium wilt is arranged, do not use this product as shown in Table 2, very low (401 ± 29.4g/ the strain of the output of watermelon and cucumber, 142 ± 48.1g/ strain), and there is not the higher (3864 ± 204g/ strain of output on the same soil of succession crop fusarium wilt, 1321 ± 125g/ strain), illustrate that the used soil continuous cropping fusarium wilt of test is very serious, the output of watermelon and cucumber is poly-after using this product on this soil increases (table 2 the 3rd row and the 2nd row ratio), they have reached does not respectively have 81% and 82% of succession crop fusarium wilt soil output, illustrates that this product is very significant overcoming aspect the succession crop fusarium wilt effect.
The extraordinary microorganism organic fertilizer that the present invention developed is to microbiotic influence on the continuous cropping soil also highly significant (seeing Table 3), and fungi quantity 0.7 * 10 on the soil of succession crop fusarium wilt is arranged as shown in Table 3
5Individual/g soil, be significantly higher than the fungi quantity (5 * 10 of the soil of no succession crop fusarium wilt
3Individual/g soil) and have that bacterial number is 2.2 * 10 on the soil of succession crop fusarium wilt
9Individual/g soil, significantly be lower than the soil bacteria quantity 5.1 * 10 of no continuous cropping obstacle
10Individual/g soil, a large amount of breedings that fungi on the continuous cropping soil (being mainly disease fungus) is described are basic factors of continuous cropping obstacles, microflora is greatly improved after using product of the present invention, and its bacterial number approaches not have the soil of continuous cropping obstacle, so is reduced to 0.8 * 10 and the quantity of fungi is poly-
4Individual/g soil, there is not significant difference with the soil of no continuous cropping obstacle.
The extraordinary biological organic fertilizer of table 2 the present invention is to the yield increasing effect (field trial) of crop on the continuous cropping fusarium wilt soil
| Watermelon fruit fresh weight (g/ strain) | Cucumber fruit fresh weight (g/ strain) | |
| CK1 (soil that the continuous cropping fusarium wilt is arranged) | 401±29.4 | 142±48.1 |
| CK2 (soil of no continuous cropping fusarium wilt) | 4771±119 | 1609±88 |
| This product of CK1+ (100kg/ mu) | 3864±204 | 1321±125 |
| This product of CK2+ (100kg/ mu) | 4993±276 | 1764±149 |
Annotate: all fertilizer are all disposable to apply
The extraordinary biological organic fertilizer of table 3 the present invention is to microbiotic influence (pot experiment) on the continuous cropping fusarium wilt soil
| Bacterium (individual/g soil) | Actinomycetes (individual/g soil) | Fungi (individual/g soil) | |
| CK1 (soil that the continuous cropping fusarium wilt is arranged) | 2.2×10 9 | 2.2×10 7 | 1.7×10 5 |
| CK2 (soil of no continuous cropping fusarium wilt) | 5.1×10 10 | 5.9×10 7 | 5.2×10 3 |
| This product of CK1+ (1.5g/kg) | 4.2×10 10 | 4.8×10 7 | 6.1×10 3 |
| This product of CK2+ (1.5g/kg) | 6.7×10 10 | 6.7×10 7 | 4.8×10 3 |
Annotate: all fertilizer are all disposable to apply
Replacing ammonium chloride with ammonium sulfate in the foregoing description is that those of ordinary skill can be accomplished, can reach same effect.
Patent of the present invention is set about from microflora and this key issue of secondary substance intoxicating thereof, develops a kind ofly can significantly eliminate the withered pathogenetic microorganism organic fertilizer product of continuous cropping, and elimination factor reaches more than 80%.The mechanism of action of this fertilizer product be function bacterium that this fertilizer product provides and effectively carbon and effectively nitrogenous source provide good condition to the cultivation of continuous cropping obstacle soil beneficial microbe fauna, make it to recover rapidly ecology and the food chain thereof of indigenous beneficial microbe and external source function bacterium microorganism; On the other hand, contain the organic nitrogen and the organic phosphor of high level in this fertilizer product, these nutriments are very favourable to the growth of crop in the continuous cropping obstacle soil, improved upright seedling rate and the anti-adversity ability of crop greatly.
Sequence table
＜110〉Agricultural University Of Nanjing
＜120〉a kind of antagonism bacterium and the microorganism organic fertilizer thereof that can prevent and kill off the succession crop fusarium wilt
＜130〉specification
<140>000
<141>2005-12-06
<160>1
<170>PatentIn version 3.1
<210>SEQ ID NO.1
<211>912
<212>DNA
＜213〉Paenibacillus polymyxa (Paenibacillus polymyxa genus)
<220>
<221>16srDNA
<222>(1)..(912)
<223>
<400>1
caccccaatc atctacccca ccttcggcgg ctggctcctt gcggttacct caccgacttc 60
gggtgttgta aactctcgtg gtgtgacggg cggtgtgtac aagacccggg aacgtattca 120
ccgcggcatg ctgatccgcg attactagca attccaactt catgtaggcg agttgcagcc 180
tacaatccga actgagaccg gcttttctag gattggctcc acctcgcggc ttcgcttccc 240
gttgtaccgg ccattgtagt acgtgtgtag cccaggtcat aaggggcatg atgatttgac 300
gtcatcccca ccttcctccg gtttgtcacc ggcagtctgc ttagagtgcc cagcttgacc 360
tgctggcaac taagcataag ggttgcgctc gttgcgggac ttaacccaac atctcacgac 420
acgagctgac gacaaccatg caccacctgt ctcctctgtc ccgaaggaaa ggcctatctc 480
tagaccggtc agagggatgt caagacctgg taaggttctt cgcgttgctt cgaattaaac 540
cacatactcc actgcttgtg cgggtccccg tcaattcctt tgagtttcag tcttgcgacc 600
gtactcccca ggcggaatgc ttaatgtgtt aacttcggca ccaagggtat cgaaacccct 660
aacacctagc attcatcgtt tacggcgtgg actaccaggg tatctaatcc tgtttgctcc 720
ccacgctttc gcgcctcagc gtcagttaca gcccagagag tcgccttcgc cactggtgtt 780
cctccacatc tctacgcatt tcacgctaca cgtgattcca ctctctctct gcactcagct 840
ccccagtttc agtgcgaccg agtgagcctc gggattaaca ccagacttaa agagcgctgc 900
gcgcgcttta cg 912
Claims (10)
1. antagonism bacterium that can overcome or eliminate the succession crop fusarium wilt, it is characterized in that, this microorganism is that the Paenibacillus polymyxa of Gram-positive belongs to the bacterial strain KKW-021 of (Paenibacillus polymyxa), be deposited at Chinese microorganism strain on September 15th, 2005 and preserve administration committee common micro-organisms center, culture presevation number is CGMCC NO.1544, and main biological property is Gram-positive (G
+), cell shape is shaft-like, the living formation gemma that expands of end, the amphimicrobian growth, the catalase positive, oxidase negative, V.P. reacting positive, the methyl red test positive, oxidizing glucose produces sour aerogenesis, oxidation wood sugar, L-arabinose, mannitol produce acid, do not utilize citrate, nitrate reductase enzyme positive, the starch hydrolysis positive, the gelatin hydrolysis feminine gender is decomposed the casein positive.
2, according to the described a kind of antagonism bacterium that can overcome or eliminate the succession crop fusarium wilt of claim 1, it is characterized in that this antagonism bacterium is to separate to obtain, and is bacillus from the plant that fallen ill and soil, by Molecular Identification, its 16srDNA sequence is SEQ.ID.NO.1.
3, describedly a kind ofly can overcome or eliminate the microorganism organic fertilizer that succession crop fusarium wilt antagonism bacterium is produced with claim 1 or 2.
4, can overcome or eliminate succession crop fusarium wilt antagonism bacteria microorganism organic manure according to claim 3 is described, it is characterized in that: contain 1 * 10 in this fertilizer
8Above antagonism bacterium quantity, the total nitrogen content weight ratio of individual/g is 4～5% (weight ratio is organic nitrogen 90% or more), and total nitrogen phosphorus potassium nutrition weight ratio is 6～10%, content of organic matter weight ratio is 30～35%.
5, describedly can overcome or eliminate succession crop fusarium wilt antagonism bacteria microorganism organic manure according to claim 3 or 4, its production method comprises:
1) selects antagonism bacterium KKW-021 for use, carry out fermenting and producing, the condition of its fermenting and producing is: pH growth scope nature, 24～41 ℃ of cultivation temperature scopes, dissolved oxygen: the throughput scope is 180～300 rev/mins under 30～100% conditions, liquid nutrient medium is that PDA adds richness, and the fermentation middle and later periods forms gemma, antimicrobial fluid bacteria containing amount short of money 〉=1 * 10 of fermentation
10Individual/ml;
2) with dry weight basis with crops straw: feces of livestock and poultry: inorganic nitrogeneous fertilizer is with 30～40: 60～70: 1～3 mixes after fermentation, treats to keep when compost temperature rises to 60-70 ℃ 5-7 days; Kept 5-7 days during compost temperature to 60 ℃, every 2-3 days turning once; Add 0.5～2% inorganic nitrogeneous fertilizer during above-mentioned each turning again; Compost temperature began to lower the temperature 3～5 days from 50-60 ℃, stopped immediately, obtained the carrier organism organic materials of antagonism bacterium;
3) add antimicrobial fluid 10-50L short of money in the SOLID ORGANIC material per ton and mix, make and contain antagonism bacterium quantity and reach 1 * 10
8The microorganism organic fertilizer that individual/g is above.
6, can overcome or eliminate succession crop fusarium wilt antagonism bacteria microorganism organic manure according to claim 5 is described, antagonism bacterium fermenting and producing temperature is 37.5 ℃ in its production method.
7, can overcome or eliminate succession crop fusarium wilt antagonism bacteria microorganism organic manure according to claim 5 is described, medium PDA adds rich middle carbohydrate and refers to glucose, sucrose, starch in its microbial fermentation production method.
8, can overcome or eliminate succession crop fusarium wilt antagonism bacteria microorganism organic manure according to claim 5 is described, used inorganic nitrogeneous fertilizer is ammonium chloride or ammonium sulfate in its production method.
9, can overcome or eliminate succession crop fusarium wilt antagonism bacteria microorganism organic manure according to claim 5 is described, used crops straw is paddy rice and the wheat stalk that is crushed to 1～5cm in its production method.
10, can overcome or eliminate succession crop fusarium wilt antagonism bacteria microorganism organic manure according to claim 5 is described, the nitrogen of the carrier organism organic materials of used antagonism bacterium is organic nitrogen more than 90% in its production method.
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| CN101456761B (en) * | 2008-12-19 | 2012-03-21 | 浙江大学 | Method for preparing microbial organic fertilizer for controlling crop blight |
| CN102851243A (en) * | 2012-09-07 | 2013-01-02 | 北京市农林科学院 | Paenibacillus polymyxa and applications thereof |
| CN103396954B (en) * | 2013-07-22 | 2014-11-19 | 河海大学 | Biological prevention and control bacterial strain for preventing and controlling rice sheath blight, biological organic fertilizer, and preparation method of biological organic fertilizer |
| CN104788143B (en) * | 2015-03-31 | 2017-09-05 | 南京农业大学 | A kind of utilization acid hydrolysis solution produces the method and product of biological organic fertilizer with decomposed manure |
| EP3205208A1 (en) * | 2016-02-09 | 2017-08-16 | Basf Se | Mixtures and compositions comprising paenibacillus strains or fusaricidins and chemical pesticides |
| CN107473881A (en) * | 2016-06-07 | 2017-12-15 | 句容市白兔镇云兔草莓专业合作社 | A kind of preparation method for the bio-feritlizer for improveing strawberry continuous cropping soil |
| CN106105450B (en) * | 2016-06-28 | 2017-05-24 | 南京农业大学 | Soil treatment and bio-organic fertilizer combined method for controlling crop wilt disease |
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| CN1074803A (en) * | 1992-11-13 | 1993-08-04 | 北京大学 | A kind of seed selection of high-efficiency crop disease prevention yield increasing fungus and production technology |
| CN1090130A (en) * | 1993-01-20 | 1994-08-03 | 河南华能科技发展公司 | Produce the method for biopesticide with bacillus licheniformis |
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|---|---|---|---|---|
| CN1074803A (en) * | 1992-11-13 | 1993-08-04 | 北京大学 | A kind of seed selection of high-efficiency crop disease prevention yield increasing fungus and production technology |
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| CN1515666A (en) * | 2000-10-11 | 2004-07-28 | 常州兰陵制药有限公司 | Method for screening pseudomonads for controlling blight |
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| 拮抗细菌诱导番茄植株抗灰霉病机理研究 童蕴慧等，植物病理学报，第36卷第6期 2004 * |
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