CN114907991B - Composite microorganism for decomposing banana straw, banana straw decomposing agent, banana straw organic fertilizer and application - Google Patents
Composite microorganism for decomposing banana straw, banana straw decomposing agent, banana straw organic fertilizer and application Download PDFInfo
- Publication number
- CN114907991B CN114907991B CN202210498590.XA CN202210498590A CN114907991B CN 114907991 B CN114907991 B CN 114907991B CN 202210498590 A CN202210498590 A CN 202210498590A CN 114907991 B CN114907991 B CN 114907991B
- Authority
- CN
- China
- Prior art keywords
- banana
- banana straw
- straw
- torches
- torch
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 235000018290 Musa x paradisiaca Nutrition 0.000 title claims abstract description 191
- 239000010902 straw Substances 0.000 title claims abstract description 170
- 241000234295 Musa Species 0.000 title claims abstract description 39
- 239000003895 organic fertilizer Substances 0.000 title claims abstract description 35
- 244000005700 microbiome Species 0.000 title claims abstract description 20
- 239000003795 chemical substances by application Substances 0.000 title abstract description 30
- 239000002131 composite material Substances 0.000 title abstract description 13
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 24
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 24
- 241000223262 Trichoderma longibrachiatum Species 0.000 claims abstract description 24
- 241000563903 Bacillus velezensis Species 0.000 claims abstract description 22
- 241000235645 Pichia kudriavzevii Species 0.000 claims abstract description 22
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 18
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims abstract description 16
- 239000004472 Lysine Substances 0.000 claims abstract description 16
- 241000187392 Streptomyces griseus Species 0.000 claims abstract description 16
- 150000001875 compounds Chemical class 0.000 claims abstract description 12
- 239000002054 inoculum Substances 0.000 claims description 53
- 239000002689 soil Substances 0.000 claims description 26
- 239000000463 material Substances 0.000 claims description 24
- 238000004321 preservation Methods 0.000 claims description 22
- 238000000855 fermentation Methods 0.000 claims description 19
- 230000004151 fermentation Effects 0.000 claims description 19
- 238000010564 aerobic fermentation Methods 0.000 claims description 14
- 241000196324 Embryophyta Species 0.000 claims description 13
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 12
- 230000001580 bacterial effect Effects 0.000 claims description 12
- 210000003608 fece Anatomy 0.000 claims description 11
- 239000010871 livestock manure Substances 0.000 claims description 11
- 238000009629 microbiological culture Methods 0.000 claims description 11
- 241000233866 Fungi Species 0.000 claims description 10
- 239000000843 powder Substances 0.000 claims description 10
- 241001465754 Metazoa Species 0.000 claims description 8
- 239000011159 matrix material Substances 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 7
- 238000009423 ventilation Methods 0.000 claims description 6
- 229920002261 Corn starch Polymers 0.000 claims description 5
- 239000008120 corn starch Substances 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 230000007480 spreading Effects 0.000 claims description 5
- 238000003892 spreading Methods 0.000 claims description 5
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 3
- 241001472591 Lysinibacillus sp. Species 0.000 claims description 3
- 239000011575 calcium Substances 0.000 claims description 3
- 229910052791 calcium Inorganic materials 0.000 claims description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 2
- 241000936706 Streptomyces griseorubens Species 0.000 claims description 2
- 239000006229 carbon black Substances 0.000 claims description 2
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 claims 1
- 239000004021 humic acid Substances 0.000 claims 1
- 239000003337 fertilizer Substances 0.000 abstract description 37
- 238000000354 decomposition reaction Methods 0.000 abstract description 21
- 239000002154 agricultural waste Substances 0.000 abstract description 4
- 230000005070 ripening Effects 0.000 abstract description 2
- 240000008790 Musa x paradisiaca Species 0.000 description 160
- 239000001963 growth medium Substances 0.000 description 52
- 238000011282 treatment Methods 0.000 description 43
- 238000009630 liquid culture Methods 0.000 description 24
- 238000000034 method Methods 0.000 description 24
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 22
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 20
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 18
- 230000001965 increasing effect Effects 0.000 description 16
- 230000004580 weight loss Effects 0.000 description 16
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 15
- 235000013312 flour Nutrition 0.000 description 14
- 230000008569 process Effects 0.000 description 14
- 239000007787 solid Substances 0.000 description 14
- 238000012360 testing method Methods 0.000 description 13
- 240000008042 Zea mays Species 0.000 description 12
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 12
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 12
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 description 12
- 229910000389 calcium phosphate Inorganic materials 0.000 description 12
- 235000005822 corn Nutrition 0.000 description 12
- 235000019691 monocalcium phosphate Nutrition 0.000 description 12
- 239000002904 solvent Substances 0.000 description 12
- 235000015099 wheat brans Nutrition 0.000 description 12
- 229910052757 nitrogen Inorganic materials 0.000 description 11
- 239000011790 ferrous sulphate Substances 0.000 description 10
- 235000003891 ferrous sulphate Nutrition 0.000 description 10
- 230000004720 fertilization Effects 0.000 description 10
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 10
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 10
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 10
- 235000019341 magnesium sulphate Nutrition 0.000 description 10
- 238000002360 preparation method Methods 0.000 description 10
- 239000011780 sodium chloride Substances 0.000 description 10
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 9
- 239000004202 carbamide Substances 0.000 description 9
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 8
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 8
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 8
- 235000011130 ammonium sulphate Nutrition 0.000 description 8
- 235000021015 bananas Nutrition 0.000 description 8
- 230000000694 effects Effects 0.000 description 8
- 238000007726 management method Methods 0.000 description 8
- 239000011591 potassium Substances 0.000 description 8
- 229910052700 potassium Inorganic materials 0.000 description 8
- 241000187747 Streptomyces Species 0.000 description 6
- 238000012258 culturing Methods 0.000 description 6
- 235000013399 edible fruits Nutrition 0.000 description 6
- 238000003306 harvesting Methods 0.000 description 6
- 239000004677 Nylon Substances 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 229920001778 nylon Polymers 0.000 description 5
- 241000609240 Ambelania acida Species 0.000 description 4
- 240000000905 Nymphoides indica Species 0.000 description 4
- 235000017590 Nymphoides indica Nutrition 0.000 description 4
- 241000282320 Panthera leo Species 0.000 description 4
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 4
- 229930006000 Sucrose Natural products 0.000 description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 4
- 238000000540 analysis of variance Methods 0.000 description 4
- 239000010905 bagasse Substances 0.000 description 4
- 210000001520 comb Anatomy 0.000 description 4
- 239000008187 granular material Substances 0.000 description 4
- 229940099596 manganese sulfate Drugs 0.000 description 4
- 239000011702 manganese sulphate Substances 0.000 description 4
- 235000007079 manganese sulphate Nutrition 0.000 description 4
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 239000002893 slag Substances 0.000 description 4
- 239000005720 sucrose Substances 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 3
- 241000222350 Pleurotus Species 0.000 description 3
- 238000005904 alkaline hydrolysis reaction Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 238000005520 cutting process Methods 0.000 description 3
- 238000013401 experimental design Methods 0.000 description 3
- 239000011574 phosphorus Substances 0.000 description 3
- 229910052698 phosphorus Inorganic materials 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- 241001653826 Pterostylis longifolia Species 0.000 description 2
- 244000061456 Solanum tuberosum Species 0.000 description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 description 2
- 241000209140 Triticum Species 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- 238000005273 aeration Methods 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000035558 fertility Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- 239000005416 organic matter Substances 0.000 description 2
- 239000001103 potassium chloride Substances 0.000 description 2
- 235000011164 potassium chloride Nutrition 0.000 description 2
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 2
- 229910052939 potassium sulfate Inorganic materials 0.000 description 2
- 235000011151 potassium sulphates Nutrition 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 241000568397 Lysinibacillus Species 0.000 description 1
- 238000000692 Student's t-test Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 239000010828 animal waste Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000292 calcium oxide Substances 0.000 description 1
- 235000012255 calcium oxide Nutrition 0.000 description 1
- 239000002361 compost Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000003864 humus Substances 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012946 outsourcing Methods 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000012353 t test Methods 0.000 description 1
- 239000000454 talc Substances 0.000 description 1
- 229910052623 talc Inorganic materials 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Fertilizers (AREA)
Abstract
The invention provides a composite microorganism for decomposing banana straw, a banana straw decomposing agent, a banana straw organic fertilizer and application, and belongs to the technical field of agricultural waste utilization. The compound microorganism comprises Streptomyces griseus three torches-09, issatchenkia orientalis three torches-06, bacillus subtilis three torches-07, bacillus lysine three torches-12, trichoderma longibrachiatum three torches-08 and Bacillus methylotrophicus three torches-04. The composite microorganism provided by the invention is used for quick ripening and decomposition of crop straws, the decomposed straws can be directly used in fields as organic fertilizers and used as base fertilizers of the next stubble, and the fertilizer has higher value and belongs to high-quality organic fertilizers.
Description
Technical Field
The invention relates to the technical field of agricultural waste utilization, in particular to a composite microorganism for decomposing banana straw, a banana straw decomposing agent, a banana straw organic fertilizer and application.
Background
Banana straw belongs to low-value agricultural waste, the water content is about 95% when the banana straw is cut down after harvest, and a large amount of water is contained, so that dry matters are relatively few, the value of the utilized matters is low according to weight, and the banana straw is not equal to income according to the conditions of cutting, sun drying and re-crushing, so that the treatment can fall into an embarrassing place, and the banana straw must be treated in a simple earlier stage, so that the banana straw treatment is not time-consuming and labor-consuming; also undeniably, the bananas are rich in potassium elements, and the straw contains a great amount of potassium elements (1.7% of the weight of the straw is potassium), so that the banana straw is quite valuable in preparing the organic fertilizer for the bananas.
The disadvantage of no treatment of straw in the field is that: 1. the stalks are returned to the field in the early stage, and after returning to the field, the compost is fermented to produce heat, acid and rot, and other elements such as nitrogen in the soil are occupied, so that a competition relationship is formed with plants, the production of the plants is frustrated, and even the plant bodies are damaged. The biological fixation can cause competition of nitrogen between microorganisms and crops, so that banana seedlings grow poorly. 2. Organic acid generated in the decomposition process of banana stalks can harm the growth of crops when accumulating to a certain concentration. The ventilation of the dry land is good, and the accumulation of organic acid is not easy; under the anaerobic condition, the banana stalk is easy to occur when a high-quantity banana stalk is applied in a paddy field with poor water permeability; 3. natural field decomposition cannot be controlled, and the unbalance of the whole field environment can be caused by improper control.
The application number is CN 201810110922-organic fertilizer and the preparation method thereof are recorded, 1. Banana straw is utilized after being crushed, dried in the sun and crushed; as for banana straw, in order to solve agricultural waste, the banana straw is treated in a most convenient and simplest mode, and the cost of operation is far greater than that of actual production through the series of operation; the raw materials are fussy to use; 2. quicklime is used in order to subsequently adjust the product to alkaline; and allowing it to absorb water and release heat to raise temperature; 3. the production process has no fermentation process, and the banana straw is mixed with other raw materials after being dried, although the banana straw is put for 30 days, the banana straw has no fermentation meaning. Therefore, how to quickly decompose banana straw and directly return to the field to be used as a base fertilizer is needed.
Disclosure of Invention
In view of the above, the invention aims to provide a composite microorganism for decomposing banana straw, a banana straw decomposing agent, a banana straw organic fertilizer and application, and the composite microorganism is used for quick-ripening and decomposition of crop straw, and the decomposed straw can be directly used in a field as an organic fertilizer, and is used as a base fertilizer of the next crop, so that the fertilizer has higher value and belongs to a high-quality organic fertilizer.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a composite microorganism for decomposing banana stalks, which comprises three torches of Streptomyces griseosus (Streptomyces griseosus), three torches of Streptomyces griseosus (Issatchenkia orientalis), three torches of Issatchenkia orientalis (Bacillus subtilis), three torches of Bacillus subtilis (Bacillus subtilis), three torches of Bacillus Lysinibacillus sp.) and B.longifolius (Trichoderma longibrachiatum), three torches of Trichoderma longibrachiatum (Trichoderma longibrachiatum), and three torches of Bacillus methylotrophicus (Bacillus methylotrophicus);
the Streptomyces griseus three-torch-09 is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of 10247;
the Issatchenkia orientalis three-torch-06 is preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC NO.2012470;
the bacillus subtilis three-torch-07 is preserved in China general microbiological culture collection center (CGMCC) with the preservation number of CGMCC NO.10248;
the bacillus lysine three-torch-12 is preserved in China Center for Type Culture Collection (CCTCC) of university of Wuhan with a preservation number of CCTCC No.2015313;
the trichoderma longibrachiatum is preserved in China general microbiological culture collection center (CGMCC) with the preservation number of 10144;
the bacillus methylotrophicus three-torch-04 is preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC NO.2012169.
Preferably, the bacterial quantity ratio of the streptomyces griseus three torches-09, the Issatchenkia orientalis three torches-06, the bacillus subtilis three torches-07, the bacillus lysine three torches-12, the trichoderma longibrachiatum three torches-08 and the bacillus methylotrophicus three torches-04 is 1:1:8:1:3:3.
The invention also provides a culture medium for culturing the composite microorganism, when the Streptomyces griseus three torches-09 is cultured, the culture medium comprises a solid culture medium and a liquid culture medium, the solid culture medium comprises wheat bran, chaff, banana straw and corn flour, and the mass ratio of the wheat bran, the chaff, the banana straw to the corn flour is 40:25:25:10; the liquid culture medium takes water as a solvent, and each liter of the liquid culture medium comprises: 10g of corn starch, 20g of banana stem juice, 2g of calcium superphosphate, 0.05g of magnesium sulfate, 0.05g of sodium chloride, 0.01g of ferrous sulfate, 3g of ammonium sulfate and pH value of 7.2;
when the Issatchenkia orientalis is-06 is cultivated, the culture medium comprises a solid culture medium and a liquid culture medium, wherein the solid culture medium comprises wheat bran, vinasse slag, banana straw and corn flour, and the mass ratio of the wheat bran to the vinasse slag to the banana straw to the corn flour is 40:25:25:10; the liquid culture medium takes water as a solvent, and each liter of the liquid culture medium comprises: 10g of wheat flour, 20g of banana stem juice, 2g of calcium superphosphate, 0.5g of magnesium sulfate, 0.05g of sodium chloride and 0.01g of ferrous sulfate, wherein the pH value is 6.8;
when the trichoderma longibrachiatum three torches-08 is cultured, the culture medium comprises a solid culture medium and a liquid culture medium, wherein the solid culture medium comprises wheat bran, bagasse, banana straw and corn flour, and the mass ratio of the wheat bran to the bagasse to the banana straw to the corn flour is 40:25:25:10; the liquid culture medium takes water as a solvent, and each liter of the liquid culture medium comprises: 10g of potato powder, 20g of banana stem juice, 2g of calcium superphosphate, 10g of sucrose and natural pH value;
when bacillus subtilis three-torch-07 is cultivated, the culture medium is a liquid culture medium, water is used as a solvent, and each liter comprises: 5g of glucose, 20g of banana stem juice, 2g of calcium superphosphate, 0.5g of magnesium sulfate, 0.5g of sodium chloride, 0.1g of manganese sulfate, 2g of ammonium sulfate, 0.01g of ferrous sulfate and pH value of 7.2;
when culturing Bacillus methylotrophicus three torches-04, the culture medium is a liquid culture medium, and water is used as a solvent, wherein each liter comprises: 5g of sucrose, 20g of banana stem juice, 2g of calcium superphosphate, 0.5g of magnesium sulfate, 0.5g of sodium chloride, 2g of ammonium sulfate, 0.01g of ferrous sulfate and pH value of 6.8;
when culturing the lysine bacillus three torches-12, the culture medium is a liquid culture medium, water is used as a solvent, and each liter comprises: 5g of soybean powder, 20g of banana stem juice, 2g of calcium superphosphate, 0.5g of magnesium sulfate, 0.5g of sodium chloride, 0.1g of manganese sulfate, 2g of ammonium sulfate, 0.01g of ferrous sulfate and pH value of 6.8.
The invention also provides a banana straw decomposing inoculant, which comprises the composite microorganism and the matrix.
Preferably, the fungus content of Streptomyces griseus tri-torch-09 in the banana straw decomposing inoculant is 1 hundred million CFU/g;
the fungus content of the Issatchenkia orientalis three torches-06 is 1 hundred million CFU/g;
the bacillus subtilis three-torch-07 has a bacterial content of 8 hundred million CFU/g;
the bacterial content of the bacillus lysine three-torch-12 is 1 hundred million CFU/g;
the fungus content of the trichoderma longibrachiatum three-torch-08 is 3 hundred million CFU/g;
the bacillus methylotrophicus three-torch-04 has a bacterial content of 3 hundred million CFU/g.
The invention also provides a banana straw organic fertilizer, which is obtained by fermenting animal manure, mushroom residues and banana straw with the banana straw decomposing inoculant.
Preferably, the mass ratio of the volume of the animal manure to the volume of the mushroom residue, the volume of the banana straw and the banana straw decomposing inoculant is 0.25m 3 :0.75m 3 :1m 3 :2kg。
Preferably, the fermentation process comprises: mixing the animal manure, the mushroom residues, the banana straw and the banana straw decomposing inoculant to obtain a mixture;
carrying out aerobic fermentation on the mixture to obtain a fermentation material; the conditions of the aerobic fermentation include: aerobic fermentation temperature is 65 ℃, ventilation is carried out for 2 times per day for 4 hours, and aerobic fermentation time is 20d;
stacking the fermented materials for 60 days, and turning the fermented materials for 1 time every 15 days to obtain the banana straw organic fertilizer.
The invention also provides application of the banana straw decomposing inoculant in banana straw returning.
Preferably, the application comprises:
crushing banana straw to obtain crushed materials;
spreading the crushed materials in the field, and then broadcasting the banana straw decomposing inoculant on the crushed materials, wherein the thickness of the spread materials is 10cm;
the dosage of the banana straw decomposing inoculant is 2 kg/mu.
The invention has the beneficial effects that:
1. the provided compound microorganism is used for quick ripening and decomposition of crop straws;
2. the decomposed straw can be used as an organic fertilizer directly in the field and used as a base fertilizer for the next stubble;
3. the banana straw organic fertilizer has higher value and belongs to high-quality organic fertilizers;
4. the microbial activity in the banana straw decomposing inoculant is good, the pertinence is strong, and the decomposition is thorough; the product has long shelf life and the bacteria can not die quickly in the storage process;
5. in the selection of a culture medium, a certain nitrogen source and all potassium sources are provided for fermentation by using banana straw juice through liquid, and in the culture of solid fermentation, blocky banana straw is added to improve the adaptability of bacteria on the banana straw;
6. the culture temperature is higher than the common culture temperature, the survival property of the cells in the decomposing inoculant at medium and high temperature is facilitated, and the cells have stronger adaptability.
Preservation description
Streptomyces griseus Sanju-09, latin Streptomyces griseorubens, was deposited at the China general microbiological center of the culture Collection management Committee of microorganisms at 12 and 29 days of 2014, and has an address of CGMCC No.10247, which is a institute of microorganisms, national academy of sciences of China, north Chen West Lu No.1, the Korean region of Beijing, and a postal code 100101;
the Issatchenkia orientalis is three torches-06, latin is Issatchenkia orientalis, and is preserved in China center for type culture collection of university of Wuhan in 2012, the address is 430072 and the preservation number is CCTCC NO.2012470;
bacillus subtilis Sanju-07, latin Bacillus subtilis, which is preserved in China general microbiological culture Collection center (CGMCC) at 29 th month of 2014, and has a address of CGMCC No.10248, which is a national institute of sciences of China, national academy of sciences of North Chen West Lu 1, korea, beijing, and a postal code 100101;
three torches-12 of lysine bacillus, latin is Lysinibacillus sp, and is preserved in China center for type culture Collection of university of Wuhan, 5 months and 18 days in 2015, with address of university of Wuhan, china, post code of 430072 and preservation number of CCTCC NO.2015313;
trichoderma longibrachiatum three torches-08, latin Trichoderma longibrachiatum and deposited in China general microbiological culture Collection center (CGMCC) on 12 months 29 of 2014, with address of China academy of sciences of China, national academy of sciences of North Chen West Lu 1, korea of Beijing, postal code 100101 and deposit number of CGMCC No.10144;
bacillus methylotrophicus three torches-04, latin Bacillus methylotrophicus, is preserved in China center for type culture Collection of university of Wuhan, 5 months and 16 days, and has a post code of 430072 and a preservation number of CCTCC NO.2012169.
Detailed Description
The invention provides a composite microorganism for decomposing banana stalks, which comprises three torches of Streptomyces griseosus (Streptomyces griseosus), three torches of Streptomyces griseosus (Issatchenkia orientalis), three torches of Issatchenkia orientalis (Bacillus subtilis), three torches of Bacillus subtilis (Bacillus subtilis), three torches of Bacillus Lysinibacillus sp.) and B.longifolius (Trichoderma longibrachiatum), three torches of Trichoderma longibrachiatum (Trichoderma longibrachiatum), and three torches of Bacillus methylotrophicus (Bacillus methylotrophicus);
the Streptomyces griseus three-torch-09 is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of 10247;
the Issatchenkia orientalis three-torch-06 is preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC NO.2012470;
the bacillus subtilis three-torch-07 is preserved in China general microbiological culture collection center (CGMCC) with the preservation number of CGMCC NO.10248;
the bacillus lysine three-torch-12 is preserved in China Center for Type Culture Collection (CCTCC) of university of Wuhan with a preservation number of CCTCC No.2015313;
the trichoderma longibrachiatum is preserved in China general microbiological culture collection center (CGMCC) with the preservation number of 10144;
the bacillus methylotrophicus three-torch-04 is preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC NO.2012169.
In the invention, the bacterial quantity ratio of the streptomyces griseus three torches-09, the Issatchenkia orientalis three torches-06, the bacillus subtilis three torches-07, the bacillus lysine three torches-12, the trichoderma longibrachiatum three torches-08 and the bacillus methylotrophicus three torches-04 is preferably 1:1:8:1:3:3.
The invention also provides a culture medium for culturing the composite microorganism, when the Streptomyces griseus three torches-09 is cultured, the culture medium comprises a solid culture medium and a liquid culture medium, the solid culture medium comprises wheat bran, chaff, banana straw and corn flour, and the mass ratio of the wheat bran, the chaff, the banana straw to the corn flour is 40:25:25:10; the liquid culture medium takes water as a solvent, and each liter of the liquid culture medium comprises: 10g of corn starch, 20g of banana stem juice, 2g of calcium superphosphate, 0.05g of magnesium sulfate, 0.05g of sodium chloride, 0.01g of ferrous sulfate, 3g of ammonium sulfate and pH value of 7.2;
when the Issatchenkia orientalis is-06 is cultivated, the culture medium comprises a solid culture medium and a liquid culture medium, wherein the solid culture medium comprises wheat bran, vinasse slag, banana straw and corn flour, and the mass ratio of the wheat bran to the vinasse slag to the banana straw to the corn flour is 40:25:25:10; the liquid culture medium takes water as a solvent, and each liter of the liquid culture medium comprises: 10g of wheat flour, 20g of banana stem juice, 2g of calcium superphosphate, 0.5g of magnesium sulfate, 0.05g of sodium chloride and 0.01g of ferrous sulfate, wherein the pH value is 6.8;
when the trichoderma longibrachiatum three torches-08 is cultured, the culture medium comprises a solid culture medium and a liquid culture medium, wherein the solid culture medium comprises wheat bran, bagasse, banana straw and corn flour, and the mass ratio of the wheat bran to the bagasse to the banana straw to the corn flour is 40:25:25:10; the liquid culture medium takes water as a solvent, and each liter of the liquid culture medium comprises: 10g of potato powder, 20g of banana stem juice, 2g of calcium superphosphate, 10g of sucrose and natural pH value;
when bacillus subtilis three-torch-07 is cultivated, the culture medium is a liquid culture medium, water is used as a solvent, and each liter comprises: 5g of glucose, 20g of banana stem juice, 2g of calcium superphosphate, 0.5g of magnesium sulfate, 0.5g of sodium chloride, 0.1g of manganese sulfate, 2g of ammonium sulfate, 0.01g of ferrous sulfate and pH value of 7.2;
when culturing Bacillus methylotrophicus three torches-04, the culture medium is a liquid culture medium, and water is used as a solvent, wherein each liter comprises: 5g of sucrose, 20g of banana stem juice, 2g of calcium superphosphate, 0.5g of magnesium sulfate, 0.5g of sodium chloride, 2g of ammonium sulfate, 0.01g of ferrous sulfate and pH value of 6.8;
when culturing the lysine bacillus three torches-12, the culture medium is a liquid culture medium, water is used as a solvent, and each liter comprises: 5g of soybean powder, 20g of banana stem juice, 2g of calcium superphosphate, 0.5g of magnesium sulfate, 0.5g of sodium chloride, 0.1g of manganese sulfate, 2g of ammonium sulfate, 0.01g of ferrous sulfate and pH value of 6.8.
In the selection of a culture medium, a certain nitrogen source and all potassium sources are provided for fermentation by using banana straw juice through liquid, and in the culture of solid fermentation, blocky banana straw is added to improve the adaptability of bacteria on the banana straw; the culture temperature is higher than the common culture temperature, the survival property of the cells in the decomposing inoculant at medium and high temperature is facilitated, and the cells have stronger adaptability. In the invention, the banana straw is preferably banana straw blocks, and the specification of the banana straw blocks is 3-5cm on the cross section. The preparation method of the banana stalk juice is not particularly limited, and conventional methods are adopted.
The invention also provides a banana straw decomposing inoculant, which comprises the composite microorganism and the matrix.
In the invention, the fungus content of Streptomyces griseus tri-09 in the banana straw decomposing inoculant is preferably 1 hundred million CFU/g; the fungus content of the Issatchenkia orientalis three torches-06 is preferably 1 hundred million CFU/g; the bacterial content of the bacillus subtilis three-torch-07 is preferably 8 hundred million CFU/g; the bacterial content of the bacillus lysine three-torch-12 is preferably 1 hundred million CFU/g; the fungus content of the trichoderma longibrachiatum three torches-08 is preferably 3 hundred million CFU/g; the bacterial content of the bacillus methylotrophicus triple torch-04 is preferably 3 hundred million CFU/g.
In the present invention, the matrix preferably comprises one or more of light calcium powder, corn starch and talc.
In the invention, the streptomyces griseus three-torch-09 and the Issatchenkia orientalis three-torch-06 are respectively added into the banana straw decomposing inoculant in the form of material powder, the trichoderma longibrachiatum three-08 is added into the banana straw decomposing inoculant in the form of spore powder, and the bacillus subtilis three-07, the bacillus methylotrophicus three-torch-04 and the bacillus lysine three-torch-12 are respectively added into the banana straw decomposing inoculant in the form of bacterial powder, and the preparation methods are shown in tables 1 and 2.
TABLE 1 preparation of Streptomyces griseus three-torch-09, issatchenkia orientalis three-torch-06 and Trichoderma longibrachiatum three-08
TABLE 2 preparation of Bacillus subtilis three torch-07, bacillus methylotrophicus three torch-04 and Bacillus lysine three torch-12
The proportion of the banana straw decomposing inoculant is shown in Table 3.
Table 3 formulation of banana straw decomposing inoculant
The invention also provides a banana straw organic fertilizer, which is obtained by fermenting animal manure, mushroom residues and banana straw with the banana straw decomposing inoculant.
In the present invention, the animal waste preferably includes pig waste. In the present invention, the mushroom residue preferably includes pleurotus geesteranus residue or agaric mushroom residue. In the invention, the mass ratio of the volume of the animal manure to the volume of the mushroom residue, the volume of the banana straw and the banana straw decomposing inoculant is preferably 0.25m 3 :0.75m 3 :1m 3 2kg. In the present invention, the fermentation method preferably comprises: mixing the animal manure, the mushroom residues, the banana straw and the banana straw decomposing inoculant to obtain a mixture; carrying out aerobic fermentation on the mixture to obtain a fermentation material; the conditions of the aerobic fermentation include: aerobic fermentation temperature is 65 ℃, ventilation is carried out for 2 times per day for 4 hours, and aerobic fermentation time is 20d; stacking the fermented materials for 60 days, and turning the fermented materials for 1 time every 15 days to obtain the banana straw organic fertilizer. In the present invention, the fermented material is preferably stacked at a temperature of 50 ℃.
The invention also provides application of the banana straw decomposing inoculant in banana straw returning.
In the present invention, the application preferably includes: crushing banana straw to obtain crushed materials; spreading the crushed materials in the field, and then broadcasting the banana straw decomposing inoculant on the crushed materials, wherein the thickness of the spread materials is 10cm; the dosage of the banana straw decomposing inoculant is 2 kg/mu. In the invention, the banana straw decomposing inoculant is preferably used together with urea, the urea is preferably used in an amount of 5 kg/mu, and the urea provides a nitrogen source for fermentation.
The invention is described in detail below in connection with examples for further illustration of the invention, but they are not to be construed as limiting the scope of the invention.
Example 1
The preparation of the banana straw decomposing inoculant is shown in tables 4-6.
TABLE 4 preparation of Streptomyces griseus three-torch-09, issatchenkia orientalis three-torch-06 and Trichoderma longibrachiatum three-08
/>
TABLE 5 preparation of Bacillus subtilis three torch-07, bacillus methylotrophicus three torch-04 and Bacillus lysine three torch-12
The proportion of the banana straw decomposing inoculant is shown in Table 6.
Table 6 proportion of Banana straw decomposition agent
The matrix comprises 40% of light calcium powder, 20% of corn starch, 15% of plant ash, 20% of humus soil and 5% of white carbon black, and the mass percentage of the matrix is calculated.
In the invention, the moisture of the banana straw decomposing inoculant is controlled below 5%.
Example 2
And (3) performing a decomposition effect experiment of the banana straw decomposition agent:
1. materials and methods
1.1 three torches banana straw decomposing inoculant straw sample, farmland where test is located and test time
The banana straw decomposing inoculant prepared in example 1. Conventional banana straw decomposing field (manufactured by Fujian Sanju biotechnology Co., ltd., according to Chinese patent CN 201510067182.9) purchased on the market;
the straw sample is obtained from banana straw harvested in Jingjingcun, south city, jingjingcun, city, and village. The farmland where the test is located is Jing town Jing city village, and the test time is 9-12 months in 2020.
1.2 instruments and consumables
1mm aperture 25X 35cm nylon mesh bag, precision 0.01g electronic balance, blast drier (temperature controllable 85+ -2deg.C).
1.3 preparation of samples
For the method of weight loss of the straw, selecting banana straw with the same weight, transversely cutting the banana straw into small pieces with the weight of 3-5cm, subpackaging the small pieces with nylon mesh bags, wherein each bag is 100g, and 50 bags (5 x 10) are prepared.
1.4 sample decomposition treatment
For the stalk weight loss method, 10 treatments were set up, which also included not returning to field 1, not applying the 3 sets of decomposition agent (10 d, 20d, 30 d) to field and applying the 3 sets of decomposition agent (10 d, 20d, 30 d), each treatment was set up to 5 replicates, i.e., each set contained 5 nylon mesh bags, each nylon mesh bag contained 100g of stalk segments (at the time of loading). For the samples returned to the field and applied with the decomposing agent group, the samples are treated according to the use instruction of the decomposing agent, and then are dispersed and buried in soil layers 5-10cm away from the surface of farmland soil together with the samples returned to the field and not applied with the decomposing agent group.
For the samples returned to the field and applied with the decomposing agent group, the samples are treated according to the use instruction of the decomposing agent, and then are dispersed and buried in soil layers 5-10cm away from the surface of farmland soil together with the samples returned to the field and not applied with the decomposing agent group.
TABLE 7 grouping case
| Test group | Marking | Returning to the field | Decomposition agent | Source of decomposing inoculant |
| Blank group | O | Not returned to the field | Is not added with | - |
| Control group | CK | Returning to the field | Is not added with | - |
| Experimental group | X1 | Returning to the field | Adding | Example 1 preparation |
| Experimental control group | X2 | Returning to the field | Adding | Market outsourcing |
1.5 returning to the field soil index Condition
Soil volume weight 1.45g/cm 3 1.12g/kg of total nitrogen, 11.5g/kg of organic matters, 41mg/kg of alkaline hydrolysis nitrogen, 15.8mg/kg of available phosphorus, 148mg/kg of quick-acting potassium, pH8.1 and 40 percent of moisture.
1.6 sample detection
For the method of the weight loss rate of the straw, 5 nylon net bags (all) are taken out from each group at the corresponding time point, the straw sections are gently washed by tap water until the dripping water is colorless, namely, the foreign matters such as soil and the like are washed clean, then the straw sections are dried for 6 hours at the temperature of 85 ℃ in an oven, and the straw sections are taken out and weighed by a balance.
1.7 data processing
For the method of weight loss of straw, 10 groups of treatment are used, each group is repeated for 5 times, the initial data is the dry weight value after the treatment of the straw, the average dry weight value N of the group which is not returned to the field is calculated, and then the weight loss W of the straw of the group which is not returned to the field and to which the decomposing agent is not applied is calculated ck ＝(M o -M ck )/M o X 100%, returning to the field and applying a set of decomposing inoculant, W x ＝(M o -M x )/M o X 100%. For the returning groups, the average value of the weight loss rate of each group can be calculated, including W ck 10d 、W ck 20d 、W ck 30d 、W x 10d 、W x 20d 、W x 30d And the average weight loss ratio W of the group to which no decomposing agent was applied ck AVE ＝(W ck 10d +W ck 20d +W ck 30d ) 3, applying a decomposing inoculant group W x AVE ＝(W x 10d +W x 20d +W x 30d )/3。
2. Results and analysis
2.1 Effect of Banana straw rotting agent application on straw weight loss Rate
As for the method of the weight loss of the straw, the average value M of the dry weight of the straw which is not returned to the field is further calculated according to the data in the table 8 o = 65.63g. The weight loss rate of the returning group is shown in Table 9, and the average weight loss rate W of the group to which no decomposing inoculant was applied ck AVE =30.2%. Applying a three-torch decomposing inoculant group W x1 AVE ＝50.3％，W x1 AVE >W ck AVE . Respectively for W ck 10d And W is equal to x1 10d ，W ck 20d And W is equal to x1 20d ，W ck 30d And W is equal to x1 30d The results of the three sets of treatment data are shown in Table 10, and the P values are less than 0.05, which indicates that the weight loss rate of the set (10 d) with the decomposition agent applied is significantly different from that of the set (10 d) without the decomposition agent applied, and the same is true for the decomposition products (20 d, 30 d).
Compared with the commercially available banana straw decomposing inoculant, W x2 AVE ＝43.8％，W x1 AVE >W x2 AVE . Respectively for W x2 10d And W is equal to x1 10d ，W x2 20d And W is equal to x1 20d ，W x2 30d And W is equal to x1 30d The results of the three sets of treatment data, as shown in table 10, all had P values less than 0.05, indicating that there was a significant difference in weight loss at (10 d) for the three torches of the decomposition agent, as compared to the commercially available decomposition agent (10 d), and also for the decomposition 20d, 30 d.
According to the relevant regulations of the quality supervision test center of microbial fertilizers and edible fungus strains of the Ministry of agriculture, the weight loss rates of the applied decomposing agent group and the non-applied decomposing agent group are obviously different for the returning samples, and the used decomposing agent can be judged to have the decomposing effect. Based on the above, the decomposition of the 10d, 20d and 30d can confirm that the three-torch banana straw decomposing agent has the decomposition effect.
Table 8 Banana straw dry weight (Unit: gram)
/>
TABLE 9 weight loss ratio of banana straw (%)
Table 10 results of t-test P values for straw weight loss rate
| Pairing group | P value |
| W ck 10d And W is equal to x1 10d | 2.67*10 -8 |
| W ck 20d And W is equal to x1 20d | 1.03*10 -6 |
| W ck 30d And W is equal to x1 30d | 9.95*10 -9 |
| W x1 10d And W is equal to x2 10d | 8.57*10 -5 |
| W x1 20d And W is equal to x2 20d | 1.08*10 -5 |
| W x1 30d And W is equal to x2 30d | 2.39*10 -9 |
If P is less than 0.01, the two sets of data are extremely significantly different;
if 0.01 < P < 0.05, the two sets of data are significantly different;
if P > 0.05, the two sets of data are not significantly different.
Example 3
Fertilizer efficiency test of banana straw decomposing inoculant on banana
The banana variety to be tested is Tianbao banana.
The soil of the test soil is red and red soil, the organic matter of the soil layer of 0-60cm is 25.69g/kg, the total nitrogen is 0.86g/kg, the alkaline hydrolysis nitrogen is 65.27mg/kg, the available phosphorus is 14.11mg/kg, the quick-acting potassium is 92.32mg/kg, and the pH value is 5.10. Soil fertility level is moderate.
The experimental design test adopts a random granule arrangement, 4 treatments are provided, and the treatment is repeated for 4 times, 30 plants are selected from each cell, and the area is about 200 square. The same type of decomposing inoculant is prepared by Fujian Santa Biotechnology Co., ltd according to Chinese patent CN 201510067182.9.
TABLE 11 experimental design
Fertilization and management record
After a round of banana harvesting in a test Tian Zaishang, cutting banana straw (the length is less than 3 cm) and uniformly spreading the cut banana straw in a field (the thickness is 10 cm), after banana seedlings are planted, uniformly mixing and stirring a straw decomposing agent and urea, uniformly spreading the mixed straw decomposing agent and urea on the straw (the straw decomposing agent and urea are respectively used for 2kg and 5kg per mu, and are combined with banana base fertilizer application, urea is not directly spread on banana seedlings when fertilization is performed, so that the banana seedlings are prevented from being burnt), water is irrigated immediately, the soil moisture content is kept to reach 60%, the banana straw is ensured to fully absorb sufficient moisture, the follow-up management is operated conventionally, and other field management is the same.
Conventional fertilization:
bananas are planted in the field in 3 months and 10 days of 2020; before transplanting, each banana plant is applied to a planting hole by 0.2 kg of golden lion-horse balanced (20-20-20) compound fertilizer, 5-10cm of crushed soil is paved on the planting hole, and then transplanting is performed; after the 1 st to 2 nd new leaves of the banana plants are transplanted and spread, the pseudostems are fertilized at a height of 50 cm. It is required to apply 0.15kg urea once a week. When the false stem of the plant exceeds 50 cm, combined with hilling to start broadcasting, the fertilizer is mainly a golden lion Ma Gaodan type (35-5-10) compound fertilizer.
In the bud-pregnant period of the bananas, the additional fertilizer is applied to the soil three times a month, the fertilizer application amount of each time is 0.15kg of the golden lion Ma Gaodan type (35-5-10) compound fertilizer and 0.1kg of potassium sulfate, and the time is needed to spray the fertilizer after each additional fertilizer application; during the growth and development period of fruits, the compound fertilizer with the weight of Shi Jinshi Ma Gaojia type (14-10-36) is applied by adding 0.25kg of each plant and then adding 0.3 kg of potassium chloride. Three times of soil topdressing are carried out each month in the first half period of fruit growth, and the topdressing is carried out at least twice a month in the second half period, but the topdressing is stopped 20-30 days before harvest;
in order to facilitate comparison management, the number of fruit combs is uniformly controlled to 9 combs.
Harvesting banana under the condition of 8-degree ripeness, measuring banana yield approximately at about 2021, 5 months and 20 days, and selecting 10 plants with the same appearance in each district for yield measurement calculation.
3 analysis of results
3.1 Banana yield
The banana yield conditions of different treatments (see Table 12) show that the average yield of treatment 1 per mu is 2888.75kg, and the yield is increased by 7.53% compared with the average yield of treatment 2 per mu of 2686.5 kg; the yield is increased by 10.41 percent compared with 2616.5kg of average yield of 3 per mu; the yield is increased by 163.15% compared with the average yield per mu of 1097.75kg of treatment 4. The average yield of treatment 2 is increased by 2.68% compared with the average yield of treatment 3 per mu, and the average yield of treatment 4 per mu is increased by 144.73%.
Table 12 table of different banana yields treated
Analysis of variance of cell yields for experimental treatments (see table 13), f= 1407.0 > F 0.01 =5.95, demonstrating that the inter-process yield difference reaches very significant levels.
TABLE 13 yield analysis of variance table
| Source of variation | Sum of squares | Degree of freedom | Mean square | F value | F 0.05 | F 0.01 |
| Processing room | 611.9 | 3 | 204.0 | 1407.0 | 3.49 | 5.95 |
| Within a granule group | 1.7 | 12 | 0.1 | |||
| Total variation | 613.6 | 15 |
The multiple comparison results indicated (see table 14): the yield difference between the treatment 1 and the treatment 2 reaches a significant level, and the yield difference between the treatment 1 and the treatment 3 and the yield difference between the treatment 4 reach a very significant level; process 2 and process 3 yields were not different, process 2 and process 4 yields were very different; the process 3 and process 4 yields were very different. The banana straw organic fertilizer and 80% conventional fertilization can remarkably improve banana yield, which is probably the banana straw raw material in the tested fertilizer, improves the adaptability of banana rhizosphere to the fertilizer, increases the fertilizer utilization rate, and promotes root system growth, thereby increasing crop nutrition and promoting yield improvement.
Table 14 multiple comparisons
Note that: LSD (light source drive) 0.05 =1.0 kg/cell, LSD 0.01 =1.8 kg/cell, "×" means via LSD 0.05 Tested as significant difference, "/x'Expressed via LSD 0.01 The differences were examined to be very significant.
Conclusion 4
The banana straw decomposing inoculant provided in the embodiment 1 is used for treating banana straw, so that the growth and yield increase of banana can be promoted, and compared with the same type of decomposing inoculant, the banana straw decomposing inoculant has an increase of 7.53%; compared with the method without using the decomposing inoculant, the yield is increased by 10.41 percent; the yield increasing effect of the product is obvious. The product has application and popularization values.
Example 4
Preparing a banana straw organic fertilizer: mixing pig manure, pleurotus geesteranus residues, banana straw and the banana straw decomposing inoculant provided in the embodiment 1, and then performing an aeration fermentation process according to an organic fertilizer production process, wherein the mass ratio of the volume of the pig manure to the volume of the mushroom residues, the volume of the banana straw and the banana straw decomposing inoculant is 0.25m 3 :0.75m 3 :1m 3 2kg; the fermentation process comprises the following steps: the fermentation temperature is 65 ℃, ventilation is carried out for 2 times per day for 4 hours, and the aerobic fermentation time is 20d; and stacking for 60 days, and turning over for 1 time every 15 days to prepare the qualified banana straw organic fertilizer.
Example 5
Fertilizer efficiency test of banana straw organic fertilizer on banana
The banana variety to be tested is Tianbao banana.
The soil to be tested is red and red soil, the organic matter in the soil layer of 0-60cm is 20.37g/kg, the total nitrogen is 0.98g/kg, the alkaline hydrolysis nitrogen is 86.45mg/kg, the available phosphorus is 12.22mg/kg, the quick-acting potassium is 100.36mg/kg, and the pH value is 4.98. Soil fertility level is moderate.
The experimental design test adopts a random granule arrangement, 4 treatments are provided, and the treatment is repeated for 4 times, 30 plants are selected from each cell, and the area is about 200 square.
Table 15 test design
Common organic fertilizer: pig manure and pleurotus geesteranus residues are added according to the volume ratio of 1:3, and the banana straw decomposing inoculant of the embodiment 1 is used for preparing qualified organic fertilizer according to an organic fertilizer production process and an aeration fermentation process. The fermentation process comprises the following steps: the fermentation temperature is 65 ℃, ventilation is carried out for 2 times per day for 4 hours, and the aerobic fermentation time is 20d; stacking again for 60d, and turning over the stack 1 time every 15 d.
Fertilization and management record
Organic fertilizer: the organic fertilizer is buried into the pit bottom together in the field planting of the bananas in the treatment 1 and the treatment 2, and can be contacted with the root of the bananas, 6kg of each banana seedling is put, and 200kg of each banana seedling is applied in a district. The fertilization amount is 80% of that of the conventional fertilization, and other management methods are consistent during the growth period.
Conventional fertilization: bananas are planted in the period of 12 days of 4 months in 2020; before transplanting, each banana plant is applied to a planting hole by 0.2 kg of golden lion-horse balanced (20-20-20) compound fertilizer, 5-10cm of crushed soil is paved on the planting hole, and then transplanting is performed; after the 1 st to 2 nd new leaves of the banana plants are transplanted and spread, the pseudostems are fertilized at a height of 50 cm. It is required to apply 0.15kg urea once a week. When the false stem of the plant exceeds 50 cm, combined with hilling to start broadcasting, the fertilizer is mainly a golden lion Ma Gaodan type (35-5-10) compound fertilizer.
In the bud-pregnant period of the bananas, the additional fertilizer is applied to the soil three times a month, the fertilizer application amount of each time is 0.15kg of the golden lion Ma Gaodan type (35-5-10) compound fertilizer and 0.1kg of potassium sulfate, and the time is needed to spray the fertilizer after each additional fertilizer application; during the growth and development period of fruits, the compound fertilizer with the weight of Shi Jinshi Ma Gaojia type (14-10-36) is applied by adding 0.25kg of each plant and then adding 0.3 kg of potassium chloride. Three times of soil topdressing are carried out each month in the first half period of fruit growth, and the topdressing is carried out at least twice a month in the second half period, but the topdressing is stopped 20-30 days before harvest;
in order to facilitate comparison management, the number of fruit combs is uniformly controlled to 9 combs.
Harvesting banana under the condition of 8-degree ripeness, measuring banana yield approximately about 25 days of 6 months of 2021, and selecting 10 plants with the same appearance in each district for yield measurement calculation.
3 analysis of results
3.1 Banana yield
The banana yield conditions of different treatments (see Table 16) show that the average yield of treatment 1 per mu is 2950.25kg, and the yield is increased by 9.2% compared with the average yield of treatment 2 per mu of 2701.75 kg; the yield is increased by 17.04% compared with 2520.75kg of average yield of 3 per mu; the yield is increased by 176.11% compared with the average yield per mu of 1068.5kg of treatment 4. The average yield of treatment 2 is increased by 7.18% compared with the average yield of treatment 3 per mu, and the average yield of treatment 4 per mu is increased by 152.85%.
TABLE 16 Table of different treatments of banana yield
Analysis of variance of the cell yields for the test treatments (see Table 17), F=2299 > F 0.01 =5.95, demonstrating that the inter-process yield difference reaches very significant levels.
TABLE 17 yield analysis of variance table
| Source of variation | Sum of squares | Degree of freedom | Mean square | F value | F 0.05 | F 0.01 |
| Processing room | 644.7 | 3 | 214.9 | 2299.1 | 3.49 | 5.95 |
| Within a granule group | 1.1 | 12 | 0.1 | |||
| Total variation | 645.9 | 15 |
The multiple comparison results indicated (see table 18): the yield difference between the treatment 1 and the treatment 2 reaches a significant level, and the yield difference between the treatment 1 and the treatment 3 and the yield difference between the treatment 4 reach a very significant level; process 2 and process 3 yields were not different, process 2 and process 4 yields were very different; the process 3 and process 4 yields were very different. The banana straw organic fertilizer and 80% conventional fertilization can remarkably improve banana yield, which is probably the banana straw raw material in the tested fertilizer, improves the adaptability of banana rhizosphere to the fertilizer, increases the fertilizer utilization rate, and promotes root system growth, thereby increasing crop nutrition and promoting yield improvement.
Table 18 multiple comparisons
Note that: LSD (light source drive) 0.05 =0.8 kg/cell, LSD 0.01 =1.5 kg/cell, "×" means via LSD 0.05 The difference was examined as significant, "×" indicated that the signal was LSD-corrected 0.01 The differences were examined to be very significant.
Conclusion 4
The effect of the organic fertilizer prepared by using banana straw is better than that of the conventional organic fertilizer, and the increase of the effect is 9.2%; compared with the conventional fertilization, the organic fertilizer prepared from banana straw has the effect of increasing the yield by 17.04%; therefore, the banana straw organic fertilizer can be judged to have the effect of increasing the yield for banana planting.
The foregoing is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. It should be noted that modifications and adaptations to the present invention may occur to one skilled in the art without departing from the principles of the present invention and are intended to be comprehended within the scope of the present invention.
Claims (6)
1. The banana straw decomposing inoculant is characterized by comprising compound microorganisms and a matrix;
the compound microorganism comprises streptomyces griseus (Streptomyces griseorubens) three torches-09, issatchenkia orientalis (Issatchenkia orientalis) three torches-06, bacillus subtilis (Bacillus subtilis) three torches-07, bacillus lysine (Lysinibacillus sp.) three torches-12, trichoderma longibrachiatum (Trichoderma longibrachiatum) three torches-08 and bacillus methylotrophicus (Bacillus methylotrophicus) three torches-04;
the Streptomyces griseus three-torch-09 is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of 10247;
the Issatchenkia orientalis three-torch-06 is preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC NO.2012470;
the bacillus subtilis three-torch-07 is preserved in China general microbiological culture collection center (CGMCC) with the preservation number of CGMCC NO.10248;
the bacillus lysine three-torch-12 is preserved in China Center for Type Culture Collection (CCTCC) of university of Wuhan with a preservation number of CCTCC No.2015313;
the trichoderma longibrachiatum is preserved in China general microbiological culture collection center (CGMCC) with the preservation number of 10144;
the bacillus methylotrophicus three-torch-04 is preserved in China Center for Type Culture Collection (CCTCC) of university of Wuhan with a preservation number of CCTCC No.2012169;
the matrix comprises 40% of light calcium powder, 20% of corn starch, 15% of plant ash, 20% of humic acid soil and 5% of white carbon black, wherein the mass percentages of the matrix are respectively;
the fungus content of Streptomyces griseus three-torch-09 in the banana straw decomposing inoculant is 1 hundred million CFU/g;
the fungus content of the Issatchenkia orientalis three torches-06 is 1 hundred million CFU/g;
the bacillus subtilis three-torch-07 has a bacterial content of 8 hundred million CFU/g;
the bacterial content of the bacillus lysine three-torch-12 is 1 hundred million CFU/g;
the fungus content of the trichoderma longibrachiatum three-torch-08 is 3 hundred million CFU/g;
the bacillus methylotrophicus three-torch-04 has a bacterial content of 3 hundred million CFU/g.
2. A banana straw organic fertilizer, which is characterized in that animal manure, mushroom residues and banana straw are fermented by the banana straw decomposing inoculant of claim 1 to obtain the banana straw organic fertilizer.
3. The banana straw organic fertilizer of claim 2, wherein the movingThe mass ratio of the volume of the excrement to the volume of the mushroom residues, the volume of the banana straw and the banana straw decomposing inoculant is 0.25m 3 :0.75m 3 :1m 3 :2kg。
4. The banana straw organic fertilizer of claim 2, wherein the fermentation process comprises: mixing the animal manure, the mushroom residues, the banana straw and the banana straw decomposing inoculant to obtain a mixture;
carrying out aerobic fermentation on the mixture to obtain a fermentation material; the conditions of the aerobic fermentation include: aerobic fermentation temperature is 65 ℃, ventilation is carried out for 2 times per day for 4 hours, and aerobic fermentation time is 20d;
stacking the fermented materials for 60 days, and turning the fermented materials for 1 time every 15 days to obtain the banana straw organic fertilizer.
5. The use of the banana straw decomposing inoculant of claim 1 in banana straw returning.
6. The application according to claim 5, characterized in that it comprises:
crushing banana straw to obtain crushed materials;
spreading the crushed materials in the field, and then broadcasting the banana straw decomposing inoculant on the crushed materials, wherein the thickness of the spread materials is 10cm;
the dosage of the banana straw decomposing inoculant is 2 kg/mu.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210498590.XA CN114907991B (en) | 2022-05-09 | 2022-05-09 | Composite microorganism for decomposing banana straw, banana straw decomposing agent, banana straw organic fertilizer and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210498590.XA CN114907991B (en) | 2022-05-09 | 2022-05-09 | Composite microorganism for decomposing banana straw, banana straw decomposing agent, banana straw organic fertilizer and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114907991A CN114907991A (en) | 2022-08-16 |
| CN114907991B true CN114907991B (en) | 2024-03-29 |
Family
ID=82766696
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210498590.XA Active CN114907991B (en) | 2022-05-09 | 2022-05-09 | Composite microorganism for decomposing banana straw, banana straw decomposing agent, banana straw organic fertilizer and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114907991B (en) |
Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1150133A (en) * | 1996-09-20 | 1997-05-21 | 北京大学 | Efficient biological compound fertilizer and its preparing process |
| CN102531766A (en) * | 2011-09-15 | 2012-07-04 | 北京世纪阿姆斯生物技术股份有限公司 | Microbial decomposing agent and production method thereof |
| CN103074272A (en) * | 2012-12-17 | 2013-05-01 | 漳州三炬生物科技有限公司 | Bacillus methylotrophicus Sanju-04 and its application |
| CN103497915A (en) * | 2013-09-22 | 2014-01-08 | 东北农业大学 | Complex microbial agent for composting fermentation and application thereof |
| CN103787708A (en) * | 2013-12-22 | 2014-05-14 | 宾阳县明翔新材料科技有限公司 | Method for producing straw decomposition agent by utilizing filter mud, cassava residues and waste alcohol mash |
| CN104651277A (en) * | 2015-02-09 | 2015-05-27 | 福建三炬生物科技股份有限公司 | Straw composting bacterium agent as well as preparation method and application thereof |
| CN105316255A (en) * | 2015-07-06 | 2016-02-10 | 福建三炬生物科技股份有限公司 | Profitable soil microorganism mixed fermentation method |
| CN106748286A (en) * | 2017-02-07 | 2017-05-31 | 福建三炬生物科技股份有限公司 | A kind of biological organic fertilizer for perchlorate of degrading and preparation method thereof |
| CN108148777A (en) * | 2018-01-10 | 2018-06-12 | 上海成土养木环保科技有限公司 | A kind of bacillus subtilis, complex micro organism fungicide, organic substrate and its preparation and application |
| CN109169079A (en) * | 2018-09-18 | 2019-01-11 | 江西农业大学 | A kind of rice mechanical transplanting seedling raising ground substance, preparation method and application |
| CN109402015A (en) * | 2018-11-20 | 2019-03-01 | 江南大学 | One plant of heat bites bacillus amyloliquefaciens and its application |
| CN110129215A (en) * | 2019-04-15 | 2019-08-16 | 漳州三炬生物技术有限公司 | A kind of complex microorganism preparations and its application |
| CN111793498A (en) * | 2020-07-31 | 2020-10-20 | 福建三炬生物科技股份有限公司 | Microbial agent and preparation method and application thereof |
| CN113135787A (en) * | 2021-03-01 | 2021-07-20 | 黑龙江省农业科学院畜牧研究所 | Rice straw decomposition agent and preparation method thereof |
| CN113214027A (en) * | 2021-05-06 | 2021-08-06 | 黑龙江省科学院微生物研究所 | Biological decomposition agent based on waste edible fungus nutrition bag base material and preparation method thereof |
-
2022
- 2022-05-09 CN CN202210498590.XA patent/CN114907991B/en active Active
Patent Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1150133A (en) * | 1996-09-20 | 1997-05-21 | 北京大学 | Efficient biological compound fertilizer and its preparing process |
| CN102531766A (en) * | 2011-09-15 | 2012-07-04 | 北京世纪阿姆斯生物技术股份有限公司 | Microbial decomposing agent and production method thereof |
| CN103074272A (en) * | 2012-12-17 | 2013-05-01 | 漳州三炬生物科技有限公司 | Bacillus methylotrophicus Sanju-04 and its application |
| CN103497915A (en) * | 2013-09-22 | 2014-01-08 | 东北农业大学 | Complex microbial agent for composting fermentation and application thereof |
| CN103787708A (en) * | 2013-12-22 | 2014-05-14 | 宾阳县明翔新材料科技有限公司 | Method for producing straw decomposition agent by utilizing filter mud, cassava residues and waste alcohol mash |
| CN104651277A (en) * | 2015-02-09 | 2015-05-27 | 福建三炬生物科技股份有限公司 | Straw composting bacterium agent as well as preparation method and application thereof |
| CN105316255A (en) * | 2015-07-06 | 2016-02-10 | 福建三炬生物科技股份有限公司 | Profitable soil microorganism mixed fermentation method |
| CN106748286A (en) * | 2017-02-07 | 2017-05-31 | 福建三炬生物科技股份有限公司 | A kind of biological organic fertilizer for perchlorate of degrading and preparation method thereof |
| CN108148777A (en) * | 2018-01-10 | 2018-06-12 | 上海成土养木环保科技有限公司 | A kind of bacillus subtilis, complex micro organism fungicide, organic substrate and its preparation and application |
| CN109169079A (en) * | 2018-09-18 | 2019-01-11 | 江西农业大学 | A kind of rice mechanical transplanting seedling raising ground substance, preparation method and application |
| CN109402015A (en) * | 2018-11-20 | 2019-03-01 | 江南大学 | One plant of heat bites bacillus amyloliquefaciens and its application |
| CN110129215A (en) * | 2019-04-15 | 2019-08-16 | 漳州三炬生物技术有限公司 | A kind of complex microorganism preparations and its application |
| CN111793498A (en) * | 2020-07-31 | 2020-10-20 | 福建三炬生物科技股份有限公司 | Microbial agent and preparation method and application thereof |
| CN113135787A (en) * | 2021-03-01 | 2021-07-20 | 黑龙江省农业科学院畜牧研究所 | Rice straw decomposition agent and preparation method thereof |
| CN113214027A (en) * | 2021-05-06 | 2021-08-06 | 黑龙江省科学院微生物研究所 | Biological decomposition agent based on waste edible fungus nutrition bag base material and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 有机物料腐熟剂在香蕉茎叶还田中的应用效果研究;梁雄 等;农业与技术;第37卷(第2期);第59页左栏第1段 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114907991A (en) | 2022-08-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109650986B (en) | Carbon-based bio-organic fertilizer for improving degraded vegetable field soil and application thereof | |
| CN103848698A (en) | Biological organic fertilizer prepared by aerobic fermentation of biogas residue and preparation method thereof | |
| CN109762765B (en) | Decomposed solid fermentation microbial inoculum and application thereof in agricultural wastes | |
| CN103194407A (en) | Straw-decomposition composite microbial preparation and preparation method thereof | |
| CN112047785A (en) | Chitin-containing environment-friendly enzyme biological fertilizer and preparation method thereof | |
| CN110590457A (en) | Biological organic bacterial fertilizer for crops and preparation method thereof | |
| CN111285742A (en) | Special biofertilizer for moderate and severe saline-alkali soil and preparation method thereof | |
| CN110540475A (en) | Production method of organic fertilizer | |
| Turgut | Organic and biofertilizers effects on the rhizosphere microbiome and spring barley productivity in northern Kazakhstan | |
| CN114195598B (en) | Microalgae fertilizer for improving soil fertility and preparation method and application thereof | |
| CN105037042A (en) | Bio-fertilizer prepared through complex microbial inoculant secondary aerobic fermentation and preparation method thereof | |
| CN108218576A (en) | A kind of production of ecological fertilizer raw material and application method | |
| CN108848837A (en) | A kind of rice-rape rotation Efficient fertilization method | |
| CN111995477A (en) | Continuous cropping resistant bio-organic fertilizer and preparation method thereof | |
| CN108949635B (en) | Bacillus mucilaginosus, microbial fertilizer, preparation method and application thereof | |
| CN115819123A (en) | Biofertilizer for improving yield of winter wheat re-sowed corn and preparation method thereof | |
| CN114907991B (en) | Composite microorganism for decomposing banana straw, banana straw decomposing agent, banana straw organic fertilizer and application | |
| Dipak et al. | Effect of integrated nutrient management on growth and yield of soybean (Glycine max L. Meril) | |
| CN108249979A (en) | A kind of ecological fertilizer raw material | |
| CN113185335A (en) | Biological organic fertilizer and preparation method thereof | |
| CN108504586B (en) | Paenibacillus softening and straw decomposing agent | |
| CN112500223A (en) | Biological organic-inorganic fertilizer suitable for safflower large golden elements and preparation method thereof | |
| CN102126875B (en) | Special-effect biological organic fertilizer and processing technology thereof | |
| CN107879823A (en) | A kind of biological organic fertilizer | |
| Jat et al. | Impact of zinc and molybdenum with manure in soybean-chickpea system in vertisols of Central India |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Chen Xiaoyan Inventor after: Chen Yiping Inventor after: Yang Fengshun Inventor after: Li Yingwu Inventor after: Fu Ruizhou Inventor after: Zhai Xiucai Inventor after: Luo Maoxi Inventor after: Chen Qinghai Inventor after: Wang Jinling Inventor after: Liu Yuzhen Inventor after: Li Ximing Inventor before: Chen Xiaoyan Inventor before: Yang Fengshun Inventor before: Li Yingwu Inventor before: Zhai Xiucai Inventor before: Luo Maoxi Inventor before: Chen Qinghai Inventor before: Wang Jinling Inventor before: Liu Yuzhen Inventor before: Li Ximing Inventor before: Chen Yiping |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant |