CN107188724A - A kind of zymotechnique of biological organic bacterial manure - Google Patents
A kind of zymotechnique of biological organic bacterial manure Download PDFInfo
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- CN107188724A CN107188724A CN201710590161.4A CN201710590161A CN107188724A CN 107188724 A CN107188724 A CN 107188724A CN 201710590161 A CN201710590161 A CN 201710590161A CN 107188724 A CN107188724 A CN 107188724A
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- 239000010871 livestock manure Substances 0.000 title claims abstract description 69
- 210000003608 fece Anatomy 0.000 title claims abstract description 67
- 230000001580 bacterial effect Effects 0.000 title claims abstract description 49
- 238000000855 fermentation Methods 0.000 claims abstract description 77
- 230000004151 fermentation Effects 0.000 claims abstract description 77
- 239000002131 composite material Substances 0.000 claims abstract description 42
- 238000009825 accumulation Methods 0.000 claims abstract description 38
- 239000000463 material Substances 0.000 claims abstract description 33
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 29
- 235000009566 rice Nutrition 0.000 claims abstract description 29
- 229920000742 Cotton Polymers 0.000 claims abstract description 28
- 235000021190 leftovers Nutrition 0.000 claims abstract description 28
- 238000012545 processing Methods 0.000 claims abstract description 21
- 235000013339 cereals Nutrition 0.000 claims abstract description 17
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims abstract description 14
- 241000287828 Gallus gallus Species 0.000 claims abstract description 13
- 238000006297 dehydration reaction Methods 0.000 claims abstract description 13
- 230000005070 ripening Effects 0.000 claims abstract description 13
- 239000002994 raw material Substances 0.000 claims abstract description 10
- 244000299507 Gossypium hirsutum Species 0.000 claims abstract 8
- 150000001875 compounds Chemical class 0.000 claims description 44
- 241000209094 Oryza Species 0.000 claims description 28
- 239000000758 substrate Substances 0.000 claims description 26
- 239000010903 husk Substances 0.000 claims description 18
- 239000003921 oil Substances 0.000 claims description 16
- 244000144977 poultry Species 0.000 claims description 14
- 241000208125 Nicotiana Species 0.000 claims description 13
- 230000000813 microbial effect Effects 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 10
- 238000002360 preparation method Methods 0.000 claims description 9
- 239000000047 product Substances 0.000 claims description 8
- 241000894006 Bacteria Species 0.000 claims description 6
- 241000233866 Fungi Species 0.000 claims description 6
- 210000004209 hair Anatomy 0.000 claims description 6
- 230000000284 resting effect Effects 0.000 claims description 6
- 239000011265 semifinished product Substances 0.000 claims description 6
- 241000894007 species Species 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 4
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 3
- 241000194107 Bacillus megaterium Species 0.000 claims description 3
- 244000063299 Bacillus subtilis Species 0.000 claims description 3
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 3
- 241000193388 Bacillus thuringiensis Species 0.000 claims description 3
- 241000193417 Brevibacillus laterosporus Species 0.000 claims description 3
- 241000187747 Streptomyces Species 0.000 claims description 3
- 229940097012 bacillus thuringiensis Drugs 0.000 claims description 3
- 238000012216 screening Methods 0.000 claims description 3
- 230000007306 turnover Effects 0.000 claims description 3
- 238000012856 packing Methods 0.000 claims description 2
- 239000000843 powder Substances 0.000 claims description 2
- 241000194108 Bacillus licheniformis Species 0.000 claims 1
- 239000003337 fertilizer Substances 0.000 abstract description 25
- 238000000034 method Methods 0.000 abstract description 13
- 235000015097 nutrients Nutrition 0.000 abstract description 9
- 235000003228 Lactuca sativa Nutrition 0.000 abstract description 8
- 241000196324 Embryophyta Species 0.000 abstract description 7
- 201000010099 disease Diseases 0.000 abstract description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 238000012360 testing method Methods 0.000 abstract description 5
- 102000002322 Egg Proteins Human genes 0.000 abstract description 4
- 108010000912 Egg Proteins Proteins 0.000 abstract description 4
- 238000005516 engineering process Methods 0.000 abstract description 4
- 210000004681 ovum Anatomy 0.000 abstract description 4
- 244000052769 pathogen Species 0.000 abstract description 3
- 230000001717 pathogenic effect Effects 0.000 abstract description 3
- 241000244203 Caenorhabditis elegans Species 0.000 abstract description 2
- 241000588724 Escherichia coli Species 0.000 abstract description 2
- 241000607479 Yersinia pestis Species 0.000 abstract description 2
- 244000052616 bacterial pathogen Species 0.000 abstract description 2
- 230000006378 damage Effects 0.000 abstract description 2
- 240000008415 Lactuca sativa Species 0.000 abstract 1
- 244000061176 Nicotiana tabacum Species 0.000 abstract 1
- 240000007594 Oryza sativa Species 0.000 abstract 1
- 241000219146 Gossypium Species 0.000 description 20
- 239000002689 soil Substances 0.000 description 15
- 230000000694 effects Effects 0.000 description 10
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- 241000208822 Lactuca Species 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 229910052757 nitrogen Inorganic materials 0.000 description 5
- 229910052698 phosphorus Inorganic materials 0.000 description 5
- 229910052700 potassium Inorganic materials 0.000 description 5
- 239000002361 compost Substances 0.000 description 4
- 230000007613 environmental effect Effects 0.000 description 4
- 230000004720 fertilization Effects 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 3
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 230000035558 fertility Effects 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 244000144972 livestock Species 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 239000003895 organic fertilizer Substances 0.000 description 3
- 239000005416 organic matter Substances 0.000 description 3
- 239000011574 phosphorus Substances 0.000 description 3
- 239000011591 potassium Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 241000283913 Bacillus coagulans DSM 1 = ATCC 7050 Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 241000082085 Verticillium <Phyllachorales> Species 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 241001498622 Cixius wagneri Species 0.000 description 1
- 241000255925 Diptera Species 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009264 composting Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 238000004332 deodorization Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethanethiol Chemical compound CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000003864 humus Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000009335 monocropping Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000006213 oxygenation reaction Methods 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 238000010422 painting Methods 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 210000004894 snout Anatomy 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The present invention relates to a kind of zymotechnique of biological organic bacterial manure, belong to biological organic bacterial manure manufacture technology field.The present invention is accumulation deposit natural-dehydration will to be carried out under chicken manure, tobacco leftovers and grain, cotton and oil processing factory leftover bits and pieces raw material normal temperature, then adds composite fermentation strain by obtained by fermenting twice, ripening；The present invention may be such that Escherichia coli, roundworm worm's ovum and some communicable germs in chicken manure by being killed during becoming thoroughly decomposed, ripening can thoroughly kill worm's ovum, harmful pathogeny body or weed seed in mixed material, so that bio-bacterial manure reaches that depth is become thoroughly decomposed, fertilizer efficiency is high.Production process is environmentally friendly, decomposed period is short, nutrient loss is few；By testing crops such as paddy rice, Lettuce and cottons, yield is largely increased, and pest and disease damage is effectively controlled.Solve inadequate using the rotten degree caused by traditional fermentation methods, it is impossible to the problem of killing pathogen and the grass-seed in raw material, fermentation process pollution environment and low fertilizer efficiency completely.
Description
Technical field
The present invention relates to a kind of zymotechnique of biological organic bacterial manure, belong to biological organic bacterial manure manufacture technology field.
Background technology
Chemical fertilizer contains abundant quick-acting nutrient, and rational use of chemical fertilizer can increase substantially crop yield,
Agricultural sustainable development is indispensable at this stage.But, lavishment chemical fertilizer also brings series of negative influence：Nutrient in soil
Out of proportion, assessing index, closure becomes strong, and permeability is deteriorated, and humus content reduction, soil hardening causes farmland
Ecological environment and soil texture have impact on the quality and yield of agricultural product to a certain extent by different degrees of destruction.Moreover,
Too much apply chemical fertilizer, crop disease and pest resisting and crop decreased capacity can be made, cause agricultural chemicals largely to be applied, not only increase agricultural into
This, and exacerbate environmental quality decline and the pollution of ecological environment.
Biological organic fertilizer nutrient is complete, being capable of improved soil, the soil hardening that improvement is caused using chemical fertilizer, improvement soil
Earth physicochemical character, enhancing soil water-retaining, fertilizer conservation, the ability of fertilizer.It is micro- in beneficial microbe and soil in biological organic bacterial manure
Biology forms mutual symbiosis Relationship With Proliferation, plays the synergy of colony, beneficial bacterium is produced greatly during growth and breeding
The metabolite of amount, promotes the decomposition and inversion of organic matter, directly or indirectly can provide a variety of nutrition and pungent for crop,
Promote and regulation and control plant growth.Meanwhile, harmful pathogen procreation can be suppressed in crop root formation advantage profitable strain, enhancing is made
The degeneration-resistant resistance against diseases of thing, reduces the disease index of continuous cropping crop, is a kind of environment-friendly type fertilizer to people, animal, Environmental security, nontoxic.
On the other hand, with the large-scale cultivation of livestock and poultry, the excrement of livestock and poultry is more and more to be also required to processing, such as chicken manure；
At present the feces of livestock and poultry such as chicken manure are fabricated to organic fertilizer conventional method mainly have drying process method, ensiling, method of chemical treatment,
Heat painting method and fermented manure method etc..Traditional compost fermentation, the time that chicken manure becomes thoroughly decomposed often needs 3~6 months, decomposition reactor system mistake
Stench is unpleasant around compost in journey, sewage trickling, and mosquito, fly grow, as pollution sources important in agricultural environment.At the same time,
Nitrogen nutrient is distributed with ammonia in digest process, so as to reduce the agricultural value of compost, fertilizer efficiency is slow.Traditional compost became thoroughly decomposed
Journey is a physiological and biochemical procedure participated in by natural microorganisms, thus research and utilization inoculating microbe is to accelerate chicken manure composting to send out
Ferment process, reduces nutrient loss, environmental protection is the major subjects of current chicken manure fermenting.Also have to animal dungs such as chicken manures at present
Just using made from traditional one time fermentation method, although the sponge dough method cycle is short, rotten degree is not uniform enough, in material
Pathogeny body or grass-seed can not kill completely, materials quality is not high, fertilizer efficiency is low, pollution environment, organic fertilizer due to become thoroughly decomposed not thoroughly,
Root is also easy to cause " burn seedlings " phenomenon when applying fertilizer.
The content of the invention
It is an object of the invention to：There is provided a kind of production process environmental protection, decomposed period is short, nutrient loss is few, depth is become thoroughly decomposed
And fertilizer efficiency is high；It is inadequate using the rotten degree caused by traditional fermentation methods to solve, it is impossible to kill completely pathogen in raw material and
The zymotechnique of grass-seed, fermentation process pollution environment and the low biological organic bacterial manure of fertilizer efficiency.
The technical scheme is that：
A kind of zymotechnique of biological organic bacterial manure, it is characterised in that：Fermentation raw material is made up of the raw material of following weight parts：
Freshly-slaughtered poultry excrement 10-20 tobacco leftovers 1-3 grain, cotton and oils processing factory leftover bits and pieces 1-5
Zymotechnique comprises the following steps：
1）, first by freshly-slaughtered poultry excrement natural-dehydration to moisture be 60%-70%；Again by under tobacco leftovers and grain, cotton and oil processing factory
After heel is crushed respectively, 10-20 mesh sieves are crossed, it is standby；
2）, above-mentioned pretreated chicken manure, tobacco leftovers and grain, cotton and oil processing factory leftover bits and pieces mutually stirred and be compounded into
Compound；
3）, compound carries out to accumulation deposit at normal temperatures, resting period summer is 10-12 days, and the resting period in winter is 28-30
My god, make compound natural-dehydration under field conditions (factors), so that the moisture content of the compound after mixture is between 58%-68%；
4）, after the completion of natural-dehydration, by compound:Composite fermentation strain is 10:1 weight ratio, is added multiple in compound
Fermented bacterium is closed, and after stirring, carries out a heap fermentation, the heap fermentation time is 13-15 days summer, and winter is 15-
18 days, compound moisture control was between 55%-65% during heap fermentation；
5）, by the compound after the completion of a heap fermentation, by compound:Composite fermentation strain is 5:1 weight adds than again
Enter composite fermentation strain, and be transferred in fermentation tank after stirring and carry out secondary fermentation, the secondary fermentation time is summer 8-10
My god, in 10-13 days winter, the moisture content of control compound is between 50%-60% during secondary fermentation；
6）, start when the mixture temperature in fermentation tank reaches 50 DEG C to turn over throwing, throwing number of times is turned over for once a day, when temperature reaches
At 70-75 DEG C, throwing is turned over daily twice, when mixture temperature no longer rises and drops to below 70 DEG C, that is, accuses secondary fermentation complete
Into；
7）, the compound that will complete secondary fermentation carry out accumulation ripening, during ripening every turning in 4-5 days once,
The ripening time is 10-20 days；The semi-finished product of biological organic bacterial manure are obtained, then by biological organic bacterial manure semi-finished product through powder
After broken, screening, metering packing, biological organic bacterial manure finished product is produced.
Described grain, cotton and oil processing factory leftover bits and pieces is cotton ash, rice husk chaff, the one of which of rape cake.
Described composite fermentation strain presses 1 by composite zymocyte original seed, strain substrate material, rice husk chaff:5:1 mass ratio warp
Four times mixing expanding species is made.
Described composite zymocyte original seed by bacillus subtilis ATCC21951, bacillus megaterium ATCC11561,
Clothing bacillus ATCC11946, bacillus thuringiensis ATCC29730, bacillus laterosporus ATCC64, Streptomyces jingyangensis
ATCC40126 and bacillus coagulans ATCC7050 are 1 in mass ratio:1:1:1:1:1:1 mixed preparing is formed.
Described strain substrate material is mixed by the raw material of following weight parts：
Freshly-slaughtered poultry excrement 10-20 tobacco leftovers 1-3 grain, cotton and oils processing factory leftover bits and pieces 1-5
The preparation process of strain substrate material and above-mentioned steps 1）, step 2）With step 3）Preparation method it is identical.
The preparation process of composite fermentation strain includes：
1）, first by composite zymocyte original seed, strain substrate material, rice husk chaff press 1:5:Heap accumulates after 1 mass ratio stirs
Put, so that microbial reproduction, the accumulation deposit time is 1-2 days, accumulation deposit temperature is 50-70 DEG C；, one-level composite zymocyte
Kind；
2）, by one-level composite fermentation strain, strain substrate material, rice husk chaff press 1:5:1 mass ratio is mixed again；Then heap
Accumulation is put, so that microbial reproduction, the accumulation deposit time is 1-2 days, accumulation deposit temperature is 50-70 DEG C；, two grades of compound hairs
Yeast-like fungi kind；
3）, by two grades of composite fermentation strains, strain substrate material, rice husk chaff press 1:5:1 mass ratio is mixed again；Then heap
Accumulation is put, so that microbial reproduction, the accumulation deposit time is 1-2 days, accumulation deposit temperature is 50-70 DEG C；, the compound hair of three-level
Yeast-like fungi kind；；
4）, by three-level composite fermentation strain, strain substrate material, rice husk chaff press 1:5:1 mass ratio is mixed again；Then heap
Accumulation is put, so that microbial reproduction, the accumulation deposit time is 1-2 days, accumulation deposit temperature is 50-70 DEG C；, the compound hair of level Four
Yeast-like fungi kind；Composite fermentation strain after i.e. four times mixing expanding species.
The beneficial effects of the present invention are：
Present invention process before fermentation using compound accumulation handle, can by the Escherichia coli in chicken manure, roundworm worm's ovum and
Some communicable germs during becoming thoroughly decomposed by being killed, and ripening can thoroughly kill the worm in mixed material
Ovum, harmful pathogeny body or weed seed so that bio-bacterial manure reaches that depth is become thoroughly decomposed, and fertilizer efficiency is high, will not cause during fertilising " burn seedlings "
Phenomenon.By the pretreatment to freshly-slaughtered poultry excrement natural-dehydration, N, P, K in freshly-slaughtered poultry excrement are on the one hand prevented using natural-dehydration method
It is lost in, saves fertilizer efficiency, on the other hand the metabolism for microorganism and fermentation process is provided and must avoided while moisture content
Because water content it is too high caused by zymotic fluid cross flow, so as to cause fertilizer efficiency reduction, pollution environment.Pass through second of fermentation process control
Material moisture processed makes that the activity of microorganism is most strong so that mixed material is heated soon, shortening fermentation time between 50%-60%.
And carried out turning over throwing processing according to the temperature of fermenting mixture material, uniform fermentation, oxygenation, the effect for the deodorization that cools can be played so that
Mixed material reaches that depth is become thoroughly decomposed, the high effect of the content of organic matter.
Embodiment
Form is described in further detail again to the above of the present invention by the following examples, but should not manage this
The scope solved as above-mentioned theme of the invention is only limitted to following embodiment, and all technologies realized based on the above of the present invention are equal
Belong to the scope of the present invention.
Composite fermentation strain presses 1 by composite zymocyte original seed, strain substrate material, rice husk chaff:5:1 mass ratio is mixed through four times
Expanding species is closed to be made.Composite zymocyte original seed is by bacillus subtilis ATCC21951, bacillus megaterium ATCC11561, lichens bud
Spore bacillus ATCC11946, bacillus thuringiensis ATCC29730, bacillus laterosporus ATCC64, Streptomyces jingyangensis
ATCC40126 and bacillus coagulans ATCC7050 are 1 in mass ratio:1:1:1:1:1:1 mixed preparing is formed.Strain substrate material
It is mixed by the raw material of following weight parts：Freshly-slaughtered poultry excrement 10-20；Tobacco leftovers 1-3；Grain, cotton and oil processing factory leftover bits and pieces 1-5；Bacterium
The preparation process of kind of substrate material is：It is 60%-70% first by freshly-slaughtered poultry excrement natural-dehydration to moisture；Again by tobacco leftovers and
After grain, cotton and oil processing factory leftover bits and pieces is crushed respectively, 10-20 mesh sieves are crossed, then by above-mentioned pretreated chicken manure, tobacco leftovers
Mutually stir the water content control of the strain substrate material being compounded into after strain substrate material, mixture with grain, cotton and oil processing factory leftover bits and pieces
System is between 58%-68%；Strain substrate stockpile is accumulated after ripening again, obtained, strain substrate material finished product；The heap of strain substrate material
Product digestion time is 10-12 days summers, 28-30 days winters.
The preparation process of composite fermentation strain includes：
1）, first by composite zymocyte original seed, strain substrate material, rice husk chaff press 1:5:Heap accumulates after 1 mass ratio stirs
Put, so that microbial reproduction, the accumulation deposit time is 1-2 days, accumulation deposit temperature is 50-70 DEG C；, one-level composite zymocyte
Kind；
2）, by one-level composite fermentation strain, strain substrate material, rice husk chaff 1:5:1 mass ratio is mixed again；Then accumulate
Storage, so that microbial reproduction, the accumulation deposit time is 1-2 days, accumulation deposit temperature is 50-70 DEG C；, two grades of composite fermentations
Strain；
3）, by two grades of composite fermentation strains, strain substrate material, rice husk chaff 1:5:1 mass ratio is mixed again；Then accumulate
Storage, so that microbial reproduction, the accumulation deposit time is 1-2 days, accumulation deposit temperature is 50-70 DEG C；, three-level composite fermentation
Strain；；
4）, by three-level composite fermentation strain, strain substrate material, rice husk chaff 1:5:1 mass ratio is mixed again；Then accumulate
Storage, so that microbial reproduction, the accumulation deposit time is 1-2 days, accumulation deposit temperature is 50-70 DEG C；, level Four composite fermentation
Strain；That is, composite fermentation strain finished product.
Specific embodiment 1~5 is listed as follows：
The zymotechnique of biological organic bacterial manure comprises the following steps：
1）, first by freshly-slaughtered poultry excrement natural-dehydration to moisture be 60%-70%；Again by under tobacco leftovers and grain, cotton and oil processing factory
After heel is crushed respectively, 10-20 mesh sieves are crossed, it is standby；
2）, above-mentioned pretreated chicken manure, tobacco leftovers and grain, cotton and oil processing factory leftover bits and pieces mutually stirred and be compounded into
Compound,
3）, compound carries out to accumulation deposit at normal temperatures, resting period summer is 10-12 days, and the resting period in winter is 28-30
My god, make compound natural-dehydration under field conditions (factors), so that the moisture content of the compound after mixture is between 58%-68%；
4）, after the completion of natural-dehydration, by compound:Composite fermentation strain is 10:1 weight ratio, is added multiple in compound
Fermented bacterium is closed, and after stirring, carries out a heap fermentation, the heap fermentation time is 13-15 days summer, and winter is 15-
18 days, compound moisture control added the purpose of composite fermentation strain, one is to make between 55%-65% during heap fermentation
Compound is further dehydrated, and two be mixture temperature is increased, to promote compound to ferment；
5）, by the compound after the completion of a heap fermentation, by compound:Composite fermentation strain is 5:1 weight adds than again
Enter composite fermentation strain, and be transferred in fermentation tank after stirring and carry out secondary fermentation, the secondary fermentation time is summer 8-10
My god, 10-13 days winter, during secondary fermentation the moisture content of control compound between 50%-60%,
6）, start when the mixture temperature in fermentation tank reaches 50 DEG C to turn over throwing, throwing number of times is turned over for once a day, when temperature reaches
At 70-75 DEG C, throwing is turned over daily twice, when mixture temperature no longer rises and drops to below 70 DEG C, that is, accuses secondary fermentation complete
Into；
7）, the compound that will complete secondary fermentation carry out accumulation ripening, during ripening every turning in 4-5 days once,
The ripening time is 10-20 days；, biological organic bacterial manure semi-finished product, by organic fungi-manure semi-finished product it is size-reduced, screening, metering
After packaging, obtain, biological organic bacterial manure finished product.
Described grain, cotton and oil processing factory leftover bits and pieces is one kind in cotton ash, rice husk chaff, rape cake.
In order to illustrate the application effect of biological organic bacterial manure of the present invention, paddy rice, Lettuce and cotton are chosen separately below and is made
Application effect experiment is carried out to the application effect of the present invention for subjects, result of the test is as follows：
Experiment one：Application effect of the biological organic bacterial manure of the present invention on paddy rice
Five pieces of soil properties of selection are consistent, the uniform medium white eel mud field of fertility, are grouped into control field 1, control field 2, experimental plot
A, experimental plot B, experimental plot C, area are 0.03 mu, and the soil nutrient test result in five groups of fields is as follows：PH value：6.15；Have
Machine matter：22.421g/kg；Full nitrogen：1.16g/kg；Full phosphorus：0.08g/kg；Full potassium：23.2g/kg；Alkali-hydrolyzable nitrogen：109.6mg/kg；
Rapid available phosphorus：7.5mg/kg；Available potassium：111.8mg/kg, and following fertilizer treatment is made to five pieces of fields：
Compare field 1（0.03 mu）：Quick-acting biological organic bacterial manure are applied by 50kg/ mus of amounts；
Compare field 2（0.03 mu）：The sterile biological organic fungi-manure of the present embodiment 2 is applied by 50kg/ mus of amounts, the sterilizing of embodiment 2 has
Machine bacterial manure is that with 150 DEG C of bakings the biological organic bacterial manure of embodiment 2 was carried out into sterilization treatment in 2 hours；
Experimental plot A（0.03 mu）：The biological organic bacterial manure of the present embodiment 2 is applied by 50kg/ mus of amounts；
Experimental plot B（0.03 mu）：The biological organic bacterial manure of the present embodiment 2 is applied by 100kg/ mus of amounts；
Experimental plot C（0.03 mu）：The biological organic bacterial manure of the present embodiment 2 is applied by 150kg/ mus of amounts；
The paddy rice experimental cultivar that five pieces of fields are applied is deep two excellent 5814, and following processing is done in five groups of experiment fields：April 30
Day sowing, rice transplanting on June 2, spacing in the rows 20.3cm, line-spacing 27.3cm, it is every mu of 1. 21 ten thousand root and stem of certain plants to add up to density.Behind June 2 whole field
Respectively press above-mentioned five pieces of fields of fertilizer treatment, July 2 dry field.Snout moth's larva is prevented and treated in rice at whole growth periods 4 times, planthopper preventing and treating 5
Secondary, ear period syndrome is prevented and treated once.September control field 1, control field 2, experimental plot A, experimental plot B, experimental plot C on the 15th take 10 respectively
Strain carries out indoor species test.September is respectively received control field 1, control field 2, experimental plot A, experimental plot B, experimental plot C paddy rice on 16th
Cut, singles, Yang Jing, dry meter production.
Different disposal influences result as follows rice yield：
Table one：Different fertilization influences on rice yield
Effective fringe of paddy rice can be effectively improved using biological organic bacterial manure of the present invention and improves rice yield.
Experiment two：Application effect of the biological organic bacterial manure of the present invention on Lettuce
Four pieces of soil properties of selection are consistent, the uniform medium field of fertility, are grouped into control field 1, control field 2, experimental plot A, examination
Field B is tested, area is 20m2, and following fertilizer treatment is made to four pieces of fields：
Compare field 1（20m2）：Quick-acting biological organic bacterial manure are applied by 50kg/ mus of amounts；
Compare field 2（20m2）：The sterile biological organic fungi-manure of the present embodiment 2 is applied by 50kg/ mus of amounts, embodiment 2 sterilizes organic
Bacterial manure is that with 150 DEG C of bakings the biological organic bacterial manure of embodiment 2 was carried out into sterilization treatment in 2 hours；
Experimental plot A（20m2）：The biological organic bacterial manure of the present embodiment 2 is applied by 50kg/ mus of amounts；
Experimental plot B（20m2）：The biological organic bacterial manure of the present embodiment 2 is applied by 100kg/ mus of amounts；
Control field 1, control field 2, experimental plot A, experimental plot B do following processing：Mark four pieces of experiment fields on April 8th, 2016,
By being raked the soil level behind above-mentioned four pieces of fields of fertilizer treatment, after seed soaks 2 hours through clear water, vernalization 1 day, April 9 is sowed,
Application rate is 1g/m2, after planting spray water, lid sunshade net.April 15, sunrise after seedling removed sunshade net, and it is true that April 15 observed two panels
Leaf, applies fertilizer on no influence of emerging.Disease control 3 times is carried out in breeding time altogether, other control measures are identical with field management.
Different fertilization is as follows to Lettuce quality and yield effect result：
Table two：Influence of the different fertilization to Lettuce quality and yield
It can promote the growth of Lettuce using biological organic bacterial manure of the present invention, improve the yield of Lettuce, improve Lettuce
Absorption to N P and K, adds the ascorbic content of plant, reduces the content of nitrite, improve the product of crop
Matter.
Experiment three：Application effect of the biological organic bacterial manure of the present invention on cotton
Six pieces of soil properties of selection are consistent, the uniform medium moisture soil field of fertility, are grouped into control
Field 1, control field 2, experimental plot A, experimental plot B, experimental plot C, experimental plot D.The soil nutrient test result in six groups of fields is such as
Under：Organic matter 1.98%, alkali-hydrolyzable nitrogen 154.4mg/kg, available phosphorus 10.9mg/kg, available potassium 109.0mg/kg, area is
30m2, and following fertilizer treatment is made to six pieces of fields：
Compare field 1（30m2）：By the quick-acting biological organic bacterial manure of 50kg/ mus of amounts；
Compare field 2（30m2）：The sterile biological organic fungi-manure of the present embodiment 2 is applied by 50kg/ mus of amounts, embodiment 2 sterilizes organic
Bacterial manure is that with 150 DEG C of bakings the biological organic bacterial manure of embodiment 2 was carried out into sterilization treatment in 2 hours；
Experimental plot A（30m2）：The biological organic bacterial manure of the present embodiment 2 is applied by 50kg/ mus of amounts；
Experimental plot B（30m2）：The biological organic bacterial manure of the present embodiment 2 is applied by 50kg/ mus of amounts；
Experimental plot C（30m2）：The biological organic bacterial manure of the present embodiment 2 is applied by 100kg/ mus of amounts；
Experimental plot D（30m2）：The biological organic bacterial manure of the present embodiment 2 is applied by 200kg/ mus of amounts；
Do following field implementation processing in six pieces of experimental plots：On May 14th, 2016 site preparation, partition, respectively by above-mentioned fertilizer treatment
Six pieces of fields, six pieces of experimental plot cottons were transplanted on May 14th, 2016, and every piece of experimental plot is colonized 66 plants（Equivalent 1467 plants/
Mu）.Six pieces of experimental plots started to receive flower on the 30th in August in 2016, and pulled out cotton stalk on November 12nd, 2016.
Table three：Different fertilization is as follows to the influence result of output of cotton and verticillium wilt：
The yield of cotton is effectively improved using biological organic bacterial manure of the present invention, the diseased plant rate of cotton verticillium wilt is reduced.
Claims (6)
1. a kind of zymotechnique of biological organic bacterial manure, it is characterised in that：Fermentation raw material is made up of the raw material of following weight parts：
Freshly-slaughtered poultry excrement 10-20, tobacco leftovers 1-3, grain, cotton and oil processing factory leftover bits and pieces 1-5；
Zymotechnique comprises the following steps：
1）, first by freshly-slaughtered poultry excrement natural-dehydration to moisture be 60%-70%；Again by under tobacco leftovers and grain, cotton and oil processing factory
After heel is crushed respectively, 10-20 mesh sieves are crossed, it is standby；
2）, above-mentioned pretreated chicken manure, tobacco leftovers and grain, cotton and oil processing factory leftover bits and pieces mutually stirred and be compounded into
Compound；
3）, compound carries out to accumulation deposit at normal temperatures, resting period summer is 10-12 days, and the resting period in winter is 28-30
My god, make compound natural-dehydration under field conditions (factors), so that the moisture content of the compound after mixture is between 58%-68%；
4）, after the completion of natural-dehydration, by compound:Composite fermentation strain is 10:1 weight ratio, is added multiple in compound
Fermented bacterium is closed, and after stirring, carries out a heap fermentation, the heap fermentation time is 13-15 days summer, and winter is 15-
18 days, compound moisture control was between 55%-65% during heap fermentation；
5）, by the compound after the completion of a heap fermentation, by compound:Composite fermentation strain is 5:1 weight adds than again
Enter composite fermentation strain, and be transferred in fermentation tank after stirring and carry out secondary fermentation, the secondary fermentation time is summer 8-10
My god, in 10-13 days winter, the moisture content of control compound is between 50%-60% during secondary fermentation；
6）, start when the mixture temperature in fermentation tank reaches 50 DEG C to turn over throwing, throwing number of times is turned over for once a day, when temperature reaches
At 70-75 DEG C, throwing is turned over daily twice, when mixture temperature no longer rises and drops to below 70 DEG C, that is, accuses secondary fermentation complete
Into；
7）, the compound that will complete secondary fermentation carry out accumulation ripening, during ripening every turning in 4-5 days once,
The ripening time is 10-20 days；The semi-finished product of biological organic bacterial manure are obtained, then by biological organic bacterial manure semi-finished product through powder
After broken, screening, metering packing, biological organic bacterial manure finished product is produced.
2. a kind of zymotechnique of biological organic bacterial manure according to claim 1, it is characterised in that：Described grain, cotton and oil adds
Factory's leftover bits and pieces is cotton ash, rice husk chaff, the one of which of rape cake.
3. a kind of zymotechnique of biological organic bacterial manure according to claim 1, it is characterised in that：Described composite fermentation
Strain presses 1 by composite zymocyte original seed, strain substrate material, rice husk chaff:5:1 mass ratio is made through four mixing expanding species.
4. a kind of zymotechnique of biological organic bacterial manure according to claim 1 or 3, it is characterised in that：Described is compound
Zymophyte original seed is by bacillus subtilis ATCC21951, bacillus megaterium ATCC11561, bacillus licheniformis
ATCC11946, bacillus thuringiensis ATCC29730, bacillus laterosporus ATCC64, Streptomyces jingyangensis ATCC40126 and solidifying
It is 1 in mass ratio to tie bacillus ATCC7050:1:1:1:1:1:1 mixed preparing is formed.
5. a kind of zymotechnique of biological organic bacterial manure according to claim 1 or 3, it is characterised in that：Described strain
Substrate material is mixed by the raw material of following weight parts：
Freshly-slaughtered poultry excrement 10-20 tobacco leftovers 1-3 grain, cotton and oils processing factory leftover bits and pieces 1-5
The preparation process of strain substrate material and the step 1 described in claim 1）, step 2）With step 3）Preparation method it is identical.
6. a kind of zymotechnique of biological organic bacterial manure according to claim 1 or 3, it is characterised in that：Described is compound
The preparation process of fermented bacterium includes：
1）, first by composite zymocyte original seed, strain substrate material, rice husk chaff press 1:5:Heap accumulates after 1 mass ratio stirs
Put, so that microbial reproduction, the accumulation deposit time is that 1-2 days accumulation deposit temperature are 50-70 DEG C；, one-level composite zymocyte
Kind；
2）, by one-level composite fermentation strain, strain substrate material, rice husk chaff press 1:5:1 mass ratio is mixed again；Then heap
Accumulation is put, so that microbial reproduction, the accumulation deposit time is 1-2 days, accumulation deposit temperature is 50-70 DEG C；, two grades of compound hairs
Yeast-like fungi kind；
3）, by two grades of composite fermentation strains, strain substrate material, rice husk chaff press 1:5:1 mass ratio is mixed again；Then heap
Accumulation is put, so that microbial reproduction, the accumulation deposit time is 1-2 days, accumulation deposit temperature is 50-70 DEG C；, the compound hair of three-level
Yeast-like fungi kind；；
4）, by three-level composite fermentation strain, strain substrate material, rice husk chaff press 1:5:1 mass ratio is mixed again；Then heap
Accumulation is put, so that microbial reproduction, the accumulation deposit time is 1-2 days, accumulation deposit temperature is 50-70 DEG C；, the compound hair of level Four
Yeast-like fungi kind；Composite fermentation strain after i.e. four times mixing expanding species.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108794188A (en) * | 2018-07-24 | 2018-11-13 | 南京禾瑞中侬农业科技有限公司 | A method of producing bio-bacterial manure by carrier of composite fertilizer |
| CN109180236A (en) * | 2018-09-19 | 2019-01-11 | 山东惠民禽乐畜牧机械有限公司 | A kind of new process of aerobic fermentation processing chicken manure |
| CN111499435A (en) * | 2020-04-24 | 2020-08-07 | 大理洱海生物肥业有限公司 | Special solid organic fertilizer for tobacco and preparation method thereof |
| CN116918644A (en) * | 2023-04-23 | 2023-10-24 | 辽宁省沙地治理与利用研究所 | Method for rapidly improving productivity of degraded sandy grassland |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103570383A (en) * | 2012-07-27 | 2014-02-12 | 孙悦迎 | Method utilizing waste and bacterium dreg generated during process of producing edible fungi from straw raw materials to produce microorganism organic bacterium fertilizer |
| CN104402547A (en) * | 2014-11-17 | 2015-03-11 | 山东益生种畜禽股份有限公司 | Immediate biological treatment method for aquaculture wastes |
| CN106883063A (en) * | 2017-04-27 | 2017-06-23 | 遵义君宇生物工程有限公司 | A kind of biological organic fertilizer and its production technology |
-
2017
- 2017-07-19 CN CN201710590161.4A patent/CN107188724B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103570383A (en) * | 2012-07-27 | 2014-02-12 | 孙悦迎 | Method utilizing waste and bacterium dreg generated during process of producing edible fungi from straw raw materials to produce microorganism organic bacterium fertilizer |
| CN104402547A (en) * | 2014-11-17 | 2015-03-11 | 山东益生种畜禽股份有限公司 | Immediate biological treatment method for aquaculture wastes |
| CN106883063A (en) * | 2017-04-27 | 2017-06-23 | 遵义君宇生物工程有限公司 | A kind of biological organic fertilizer and its production technology |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108794188A (en) * | 2018-07-24 | 2018-11-13 | 南京禾瑞中侬农业科技有限公司 | A method of producing bio-bacterial manure by carrier of composite fertilizer |
| CN109180236A (en) * | 2018-09-19 | 2019-01-11 | 山东惠民禽乐畜牧机械有限公司 | A kind of new process of aerobic fermentation processing chicken manure |
| CN111499435A (en) * | 2020-04-24 | 2020-08-07 | 大理洱海生物肥业有限公司 | Special solid organic fertilizer for tobacco and preparation method thereof |
| CN116918644A (en) * | 2023-04-23 | 2023-10-24 | 辽宁省沙地治理与利用研究所 | Method for rapidly improving productivity of degraded sandy grassland |
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