CN107354105A - A kind of complex microbial inoculum and preparation method thereof and the system for administering sewage - Google Patents
A kind of complex microbial inoculum and preparation method thereof and the system for administering sewage Download PDFInfo
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- CN107354105A CN107354105A CN201710545297.3A CN201710545297A CN107354105A CN 107354105 A CN107354105 A CN 107354105A CN 201710545297 A CN201710545297 A CN 201710545297A CN 107354105 A CN107354105 A CN 107354105A
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- microbial inoculum
- aeration
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F7/00—Aeration of stretches of water
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/006—Apparatus and plants for the biological treatment of water, waste water or sewage details of construction, e.g. specially adapted seals, modules, connections
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2301/00—General aspects of water treatment
- C02F2301/08—Multistage treatments, e.g. repetition of the same process step under different conditions
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The invention discloses a kind of complex microbial inoculum and preparation method thereof and the system for administering sewage, complex microbial inoculum includes each strain of following percentage by weight：Bacillus amyloliquefaciens 20~35%, bacillus subtilis 25~40%, bacillus licheniformis 15~30% and colloid bacillus cereus 20~30%.The complex microbial inoculum of the present invention can effectively handle domestic sewage in rural areas, remove each pollutant in sewage.By rationally designing the system for administering sewage, in first order degraded pond, mix bacterium agent is added；In every one-level degraded pond, aeration tube is equipped with, step by step overflow, level level aeration, allows sewage in every low-level culture pool, can there is contact microbial inoculum, the chance that fully degraded is decomposed.
Description
Technical field
The invention belongs to sanitary sewage disposal technical field, and in particular to a kind of complex microbial inoculum and preparation method thereof
With the system for administering sewage, it is applied to small volume, high concentration, the sanitary sewage degraded discontinuously discharged.
Background technology
At present, the subject matter amount of being of the sanitary sewage in China rural area it is big, it is wide, put more, scattered, sanitary sewage and kitchen
Remaining, excrement mixing, typically only does simple precipitation, and some is even in line.Pathogen, COD, ammonia nitrogen, total nitrogen, total phosphorus in sewage etc.
It is exceeded, do not reach discharge standard.If by the processing mode of municipal wastewater, pipe network collection is built, sewage treatment plant is built and is uniformly processed.
So invest huge, construction period length, operation cost is high, is difficult to for the moment.How could in a short time, with limited money
Gold, rural area is so measured sanitary sewages big, wide, that point is more, scattered, obtain effectively, sustainable improvement, be that we are urgent
Need to solve the problems, such as.
The content of the invention
It is contemplated that at least solves one of technical problem present in prior art.Therefore, present invention offer is a kind of micro-
Biological compound fungi agent and preparation method thereof and the system for administering sewage, it is therefore an objective to which effectively degraded has containing food waste, excrement etc. are a variety of
The sanitary sewage of machine discarded object.
To achieve these goals, the technical scheme taken of the present invention is：
A kind of complex microbial inoculum, include each strain of following percentage by weight：
Bacillus amyloliquefaciens 20~35%, bacillus subtilis 25~40%, bacillus licheniformis 15~30% and glue
Matter bacillus 20~30%.
The present invention also provide complex microbial inoculum preparation method, the preparation method be by bacillus amyloliquefaciens,
Bacillus subtilis, bacillus licheniformis and colloid bacillus cereus respectively successively after inoculated and cultured, volume production culture, according to
Strain after each volume production culture is made side's amount after intersecting antagonism culture.
The inoculated and cultured is that first 36~48h of inoculated and cultured on solid medium, cultivation temperature are 25~32 DEG C；Afterwards
36~48h of inoculated and cultured on liquid medium within, cultivation temperature are 28~30 DEG C, and shaking flask speed is 50~70r/min.
The solid medium is agar.
The composition of the fluid nutrient medium is：Contain sucrose 15g, glucose 12g, K per L2HPO40.3g, CaCO3
1.5g, NaCl 0.3g.
The volume production culture is that the strain after fluid nutrient medium culture is inoculated in fermentation tank to cultivate, in the fermentation tank
The composition of fermentation medium is：30~50g/L of glucose, 70~100g/L of molasses, urea 5~10g/L, KH2PO45~8g/L,
Remaining is distilled water；PH value 6.8~7.0.
The condition of the volume production culture is：Strain inoculum concentration is 3~10%；Less than 30 DEG C culture 120h, in incubation
The stirring 5min per 12h, the inflation 10min per 2h, after culture, bacterium number reaches 10~15 × 10 in zymotic fluid10/ml。
It is described intersection antagonism culture used medium composition be：20~40g/L of glucose, 30~50g/L of molasses, urea 10
~12g/L, P2O55~8g/L, KH2PO42~5g/L, remaining is distilled water；PH value 6.8~7.0.
It is described intersect antagonism culture condition be：Stirring 5min, the inflation per 2h per 4h in incubation in incubation
10min, co-culture 120h；It is 5~8 × 10 to intersect viable count in the mixed liquor of antagonism culture10Cfu/ml, as finished product.
The system that sewage is administered using the complex microbial inoculum, including sewage degraded pond and for sewage degraded pond
The aerator of aeration, the sewage degraded pond include the multistage overflow degraded pond that is sequentially connected, between adjacent overflow degraded pond
Separated by dividing plate, and the height of dividing plate is from the water inlet end of sewage degraded pond importing complex microbial inoculum to water outlet end direction
Reduce successively.
The bottom face of the overflow degraded pond is the inclined plane structure tilted down from water inlet end to water outlet extreme direction.
The aerator includes aeration source, appendix and aeration tube, and the aeration source is connected by appendix and aeration tube
Connect, the aeration tube is in the bottom side of overflow degraded pond introversion ramp structure.
The aeration source is electromagnetic air pump, and the aeration quantity of the aeration tube is 200~300L/min, and aeration time is
8~15min.Preferably, the aeration quantity of aeration tube is 280L/min.
In first order overflow degraded pond, monthly by 0.08~0.15L/M of sewage quantity3Input amount, add micro- life
Thing composite bacteria agent, it is aerated daily twice.Preferably, respectively once it is aerated sooner or later for each overflow degraded pond daily, every time aeration
10min。
Multiple solarization air caps are evenly equipped with the length direction of the aeration tube, the outer surface of the aeration tube is provided with protection network.
The system also includes motor-driven mechanism and for by the solid pollution on overflow degraded pond introversion ramp structure
Thing is oriented to the scraper plate of inclined plane structure bottom, and the madial wall of the overflow degraded pond is provided with slide rail, and the motor-driven mechanism can
Scraper plate is driven to be moved back and forth along slide rail.
In the composite bacteria agent of the present invention, bacillus amyloliquefaciens (Bacillusamyloliquefaciens)；To amine salt
There is obvious degradation, remove the pollution of all kinds of detergent.Bacillus subtilis (Bacillus subtilis)；Kill disease
Opportunistic pathogen, decompose degraded excrement type organic.Bacillus licheniformis (Bacillu licheniformis)；Decompose having in water
Malicious harmful substance, purifies water；With stronger protease, lipase, amylase activity, organic dirt caused by food waste can be decomposed
Dye.Colloid bacillus cereus (Bacill mucitaginosus)；Fixed nitrogen, phosphorus decomposing, potassium decomposing, it will can produce rancid excrement, kitchen
It is remaining to wait organic matter, resolve into the small molecule nutrients that can be absorbed by water plant, planktonic organism.
Beneficial effects of the present invention：The complex microbial inoculum of the present invention can effectively handle domestic sewage in rural areas, remove
Each pollutant in sewage.By rationally designing the system for administering sewage, in first order degraded pond, mix bacterium agent is added；
In every one-level degraded pond, aeration tube is equipped with, step by step overflow, level level aeration, allows sewage to have contact in every low-level culture pool
Microbial inoculum, the chance that fully degraded is decomposed.So that the water body flowed out from end overflow degraded pond, at least through more than 30 days
After stationary stream, overflow, aeration degraded, water outlet reaches《Urban wastewater treatment firm pollutant emission standard》In GB18918-2002
One-level A standards are discharged.Bottom face is arranged to inclined plane structure in each overflow degraded pond, is easy to play water body flow direction and leads
To effect, and the solid matter in sewage is allowd to be easy to collect progress as current pour the bottom side of inclined plane
Follow-up processing.It is aerated tube outer surface and protection network is set, avoid pollutant blocking aeration hole, influences aeration tube aeration.The present invention
Invest small, quick, cost is low, and operation is simple, is a kind of practicable method.
Brief description of the drawings
This specification includes the following drawings, and shown content is respectively：
Fig. 1 is the structural representation of the present invention；
Fig. 2 is that the degraded pond of the present invention sets the front view of capping；
Fig. 3 is the partial structural diagram that scraper plate is set in degraded pond of the present invention.
In figure mark for：
1st, overflow degraded pond, 2, dividing plate, 3, inclined plane structure, 4, aeration source, 5, appendix, 6, aeration tube, 7, scraper plate, 8,
Slide rail.
Embodiment
Below against accompanying drawing, by the description to embodiment, embodiment of the invention is made further details of
Explanation, it is therefore an objective to help those skilled in the art to have more complete, accurate and deep reason to design of the invention, technical scheme
Solution, and contribute to its implementation.
A kind of complex microbial inoculum, include each strain of following percentage by weight：
Bacillus amyloliquefaciens 20~35%, bacillus subtilis 25~40%, bacillus licheniformis 15~30% and glue
Matter bacillus 20~30%.
The preparation method of the complex microbial inoculum is by bacillus amyloliquefaciens, bacillus subtilis, lichens gemma bar
Bacterium and colloid bacillus cereus are respectively successively after inoculated and cultured, volume production culture, according to formula ratio by the bacterium after each volume production culture
Kind is made after intersecting antagonism culture.
Its specific preparation method comprises the following steps：
A, bacillus amyloliquefaciens, bacillus subtilis, bacillus licheniformis, colloid bacillus cereus are seeded in respectively solid
Body culture medium, cultivates 36~48h, and cultivation temperature is 25~32 DEG C；Solid medium uses agar.
B, the strain after step A is cultivated, is inoculated in shaking flask fluid nutrient medium respectively, cultivates 36~48h, cultivation temperature
For 28~30 DEG C, shaking flask speed is 50~70r/min；Wherein, the composition of fluid nutrient medium is：Contain sucrose 15g, Portugal in per L
Grape sugar 12g, K2HPO40.3g, CaCO31.5g,NaCl 0.3g.The fluid nutrient medium of bacterium is identical in four.
C, the strain after step B is cultivated is inoculated in fermentation tank respectively, carries out volume production culture.Fermentation medium components
For：30~50g/L of glucose, 70~100g/L of molasses, urea 5~10g/L, KH2PO45~8g/L, remaining is distilled water；PH
Value 6.8~7.0；Volume production condition of culture is：Strain inoculum concentration is 3~10%；Less than 30 DEG C culture 120h, it is every in incubation
12h stirs 5min, the inflation 10min per 2h.After culture, bacterium number reaches 10~15 × 10 in zymotic fluid10/ml。
D, the strain after step C volume production cultures is mixed according to above-mentioned formula ratio, intersects antagonism culture, you can micro- life is made
Thing composite bacteria agent.Intersecting antagonism culture used medium composition is：20~40g/L of glucose, 30~50g/L of molasses, urea 10
~12g/L, P2O55~8g/L, KH2PO42~5g/L, remaining is distillation water ph value 6.8~7.0.Condition of culture is：Cultivated
Per 4h, stirring 5min, every 2h inflate 10min, co-culture 120h in journey.Viable count is 5~8 × 10 in mixed liquor10Cfu/ml, i.e.,
For finished product.
As shown in Fig. 1 to 2, for the ease of handling domestic sewage in rural areas, there is provided one kind is compound using microorganism
The system that microbial inoculum administers sewage, including sewage degraded pond and the aerator for being aerated to sewage degraded pond, sewage degraded pond
Including the multistage overflow degraded pond being sequentially connected, separated between adjacent overflow degraded pond 1 by dividing plate 2, and the height of dividing plate 2 from
The water inlet end that sewage degraded pond imports complex microbial inoculum reduces successively to water outlet end direction.It is arranged such the height of dividing plate
Degree, it is easy to the sanitary sewage overflow step by step imported, and is aerated by level level, allows sewage in every one-level overflow degraded pond, can
There are contact microbial inoculum, the chance that fully degraded is decomposed.Multistage overflow degraded pond can be multistage overflow degraded pond more than two level, only
Using an overflow degraded pond can also, but the effect that its degraded is decomposed is not so good as using multistage overflow degraded more than two level
Pond.Considering cost and degradation effect, it is preferred to use level Four overflow degraded pond.
Flow guiding structure can be set in the water inlet end of first order overflow degraded pond, flow guiding structure can be to be arranged on the first order
The trench structure on side top where overflow degraded pond water inlet end, trench structure is from the outside of overflow degraded pond side to interior side
To the drainage trough to tilt down, drainage trough can be multiple, be easy to sewage drainage to be oriented in first order overflow degraded pond.Most
The water outlet end of rear stage overflow degraded pond can set flow-guiding structure, be passed through by the sewage of this flow-guiding structure fully degraded
Cross afterbody overflow degraded pond and be oriented to collecting-tank, be easy to follow-up processing.Flow-guiding structure includes deflector and is sealingly fastened in
The baffle plate of the side of deflector two, both connected modes can be used and be welded to connect, and this flow-guiding structure is easy to sewage after degraded
Play drainage.It is of course also possible to set capping plate on the top of two baffle plates, the excessive spilling of current is avoided, while can avoid
External substance is fallen into this flow-guiding structure.
As a further improvement, the bottom face of overflow degraded pond is to incline from water inlet end to what water outlet extreme direction tilted down
Ramp structure 3, it is therefore an objective to be easy to play the guiding role to water body flow direction, and allow solid matter in sewage with
Current pour the bottom side of inclined plane, are easy to collect the follow-up processing of progress.When needing processing, it is only necessary to by overflow degraded pond
After interior water export, solid pollutant therein is taken out.The inclination angle of inclined plane is 10~30 °.Overflow degraded pond it is upper
End is preferably provided lid, avoids under fortuitous event, and environmental contaminants enter in pond from overflow degraded pond top, pollutes existing
As.
Aerator includes aeration source 4, appendix 5 and aeration tube 6, and aeration source 4 is connected by appendix 5 with aeration tube 6,
Aeration tube 6 is in the bottom side of the introversion ramp structure of overflow degraded pond 1.During specific setting, appendix is fixed on overflow drop
Xie Chi outer avris, the side of overflow degraded pond set sealing guide channel, one end of aeration tube through sealing guide channel with
Appendix connection connection, seals the setting of guide channel, avoids the sewage in overflow degraded pond from being flowed out out of this guide channel, real
Shi Shi, flexible sealing gum cover can be set at the both ends of sealing guide channel, aeration tube interference is caught in flexible sealing gum cover, had
Effect prevents drainage.One-way control valve is set in the connection end of each aeration tube and appendix, avoids sewage from importing gas transmission
In pipeline.In addition, a flow control valve can be separately provided in the connection end of each aeration tube and appendix, it is easy to phase
The overflow degraded pond answered carries out the adjustment of aeration quantity, i.e., according to the specific needs of each overflow degraded pond, carries out turning aeration quantity down
Or increase aeration quantity, adaptability are stronger.
Aeration source 4 is electromagnetic air pump, and the aeration quantity of aeration tube 6 is 200~300L/min, aeration time is 8~
15min.Preferably, the aeration quantity of aeration tube is 280L/min.
In first order overflow degraded pond, monthly by 0.08~0.15L/M of sewage quantity3Input amount, add above-mentioned micro- life
Thing composite bacteria agent, it is aerated daily twice.Preferably, respectively once it is aerated sooner or later for each overflow degraded pond daily, every time aeration
10min.Preferably, by sewage quantity 0.1L/M3Input amount add complex microbial inoculum.
Multiple solarization air caps are evenly equipped with the length direction of aeration tube 6, the outer surface of aeration tube 6 is provided with protection network.Lay
The uniformity that multiple solarization air caps ensure aeration is put, protection network can use mud retaining net, avoid beating fast solid pollutant blocking aeration
Hole, influence to be aerated.
As shown in figure 3, as a further improvement, the system also includes motor-driven mechanism and for by overflow degraded pond
Solid pollutant on introversion ramp structure is oriented to the scraper plate 7 of inclined plane structure bottom, and the madial wall of overflow degraded pond, which is provided with, to be slided
Rail 8, motor-driven mechanism can drive scraper plate 7 to be moved back and forth along slide rail 8.Motor-driven mechanism can use side of the prior art
Method is realized, drives scraper plate to be moved along slide rail by motor-driven mechanism, when specifically setting, the side wall centre position of scraper plate and slide rail
Connect and can be moved along slide rail.In order to avoid the two side of scraper plate unnecessary fiber crops are caused with overflow degraded pond inwall phase mutual friction
It is tired, can be along sliding block be set on slide rail, scraper plate is connected by sliding block with slide rail, passes through motor-driven mechanism band movable slider and scraper plate
Moved along slide rail.Current are formed with larger resistance in order to reduce scraper plate, the plate face of scraper plate can set multiple leaking holes, and
And less distance is left between the bottom of scraper plate and inclined plane structure, it is easy to slide, and be easy to part of contaminated water to flow from bottom
It is logical, when solid pollutant tires out certain thickness, it can carry out scraping push operation by scraper plate, solid pollutant is oriented to inclined plane
Bottom side.
It is described in detail below by specific embodiment：
Embodiment 1
One kind administers sanitary sewage complex microbial inoculum, includes each strain of following percentage by weight：
Bacillus amyloliquefaciens 20%, bacillus subtilis 30%, bacillus licheniformis 20% and colloid bacillus cereus
30%.
Its preparation method comprises the following steps：
A, bacillus amyloliquefaciens, bacillus subtilis, bacillus licheniformis, colloid bacillus cereus are seeded in respectively solid
Body culture medium, cultivates 36~48h, and cultivation temperature is 25 DEG C；Solid medium uses agar.
B, the strain after step A is cultivated, is inoculated in shaking flask fluid nutrient medium respectively, cultivates 36~48h, cultivation temperature
For 28 DEG C, shaking flask speed is 60r/min；Wherein, the composition of fluid nutrient medium is：Contain sucrose 15g, glucose 12g in per L,
K2HPO40.3g, CaCO31.5g, NaCl 0.3g.The fluid nutrient medium of bacterium is identical in four.
C, the strain after step B is cultivated is inoculated in fermentation tank respectively, carries out volume production culture.Fermentation medium components
For：30~50g/L of glucose, 70~100g/L of molasses, urea 5~10g/L, KH2PO45~8g/L, remaining is distilled water；PH
Value 6.8~7.0；Volume production condition of culture is：Strain inoculum concentration is 3~10%；Less than 30 DEG C culture 120h, it is every in incubation
12h stirs 5min, the inflation 10min per 2h.After culture, bacterium number reaches 10~15 × 10 in zymotic fluid10/ml。
D, the strain after step C volume production cultures is mixed according to above-mentioned formula ratio, intersects antagonism culture, you can micro- life is made
Thing composite bacteria agent.Intersecting antagonism culture used medium composition is：20~40g/L of glucose, 30~50g/L of molasses, urea 10
~12g/L, P2O55~8g/L, KH2PO42~5g/L, remaining is distillation water ph value 6.8~7.0.Condition of culture is：Cultivated
Per 4h, stirring 5min, every 2h inflate 10min, co-culture 120h in journey.Viable count is 5~8 × 10 in mixed liquor10Cfu/ml, i.e.,
For finished product.
Administered using composite bacteria agent in the system of sewage, using level Four overflow degraded pond, 5M is monthly produced by each household3Sewage
Calculate, the specific size of design level Four overflow degraded pond is specially per a length of 2m of one-level degraded pond, a width of 2m, first order overflow
The water inlet end of degraded pond is highly 1.8m, and the water side of fourth stage overflow degraded pond is highly 1.4m, is degraded from first order overflow
The height of central dividing plate between pond level Four overflow on earth degraded pond is followed successively by 1.7m, 1.6m, 1.5m, the inclined plane knot of bottom face
The inclination angle of structure is 20 °, and source of the gas uses 400W electromagnetic air pumps, and the aeration quantity of aeration tube 6 is 280L/min, monthly by sewage
Measure 0.1L/M3Input amount, add complex microbial inoculum, be respectively once aerated sooner or later for four overflow degraded ponds daily,
Each 10min.
Embodiment 2
One kind administers sanitary sewage complex microbial inoculum, includes each strain of following percentage by weight：
Bacillus amyloliquefaciens 30%, bacillus subtilis 35%, bacillus licheniformis 15% and colloid bacillus cereus
20%.
One kind administers sanitary sewage complex microbial inoculum, includes each strain of following percentage by weight：
Bacillus amyloliquefaciens 20%, bacillus subtilis 30%, bacillus licheniformis 20% and colloid bacillus cereus
30%.
Its preparation method comprises the following steps：
A, bacillus amyloliquefaciens, bacillus subtilis, bacillus licheniformis, colloid bacillus cereus are seeded in respectively solid
Body culture medium, cultivates 36~48h, and cultivation temperature is 32 DEG C；Solid medium uses agar.
B, the strain after step A is cultivated, is inoculated in shaking flask fluid nutrient medium respectively, cultivates 36~48h, cultivation temperature
For 30 DEG C, shaking flask speed is 60r/min；Wherein, the composition of fluid nutrient medium is：Contain sucrose 15g, glucose 12g in per L,
K2HPO40.3g, CaCO31.5g, NaCl 0.3g.The fluid nutrient medium of bacterium is identical in four.
C, the strain after step B is cultivated is inoculated in fermentation tank respectively, carries out volume production culture.Fermentation medium components
For：30~50g/L of glucose, 70~100g/L of molasses, urea 5~10g/L, KH2PO45~8g/L, remaining is distilled water；PH
Value 6.8~7.0；Volume production condition of culture is：Strain inoculum concentration is 3~10%；Less than 30 DEG C culture 120h, it is every in incubation
12h stirs 5min, the inflation 10min per 2h.After culture, bacterium number reaches 10~15 × 10 in zymotic fluid10/ml。
D, the strain after step C volume production cultures is mixed according to above-mentioned formula ratio, intersects antagonism culture, you can micro- life is made
Thing composite bacteria agent.Intersecting antagonism culture used medium composition is：20~40g/L of glucose, 30~50g/L of molasses, urea 10
~12g/L, P2O55~8g/L, KH2PO42~5g/L, remaining is distillation water ph value 6.8~7.0.Condition of culture is：Cultivated
Per 4h, stirring 5min, every 2h inflate 10min, co-culture 120h in journey.Viable count is 5~8 × 10 in mixed liquor10Cfu/ml, i.e.,
For finished product.
Administered using composite bacteria agent in the system of sewage, using level Four overflow degraded pond, 6M is monthly produced by each household3Sewage
Calculate, the specific size of design level Four overflow degraded pond is specially per a length of 3m of one-level degraded pond, a width of 2m, first order overflow
The water inlet end of degraded pond is highly 1.8m, and the water side of fourth stage overflow degraded pond is highly 1.4m, is degraded from first order overflow
The height of central dividing plate between pond level Four overflow on earth degraded pond is followed successively by 1.7m, 1.6m, 1.5m, the inclined plane knot of bottom face
The inclination angle of structure is 30 °, and source of the gas uses 500W electromagnetic air pumps, and the aeration quantity of aeration tube 6 is 280L/min, monthly by sewage
Measure 0.12L/M3Input amount, add complex microbial inoculum, be respectively once aerated sooner or later for four overflow degraded ponds daily,
Each 10min.
The present invention is exemplarily described above in association with accompanying drawing.Obviously, present invention specific implementation is not by above-mentioned side
The limitation of formula.As long as employ the improvement of the various unsubstantialities of inventive concept and technical scheme of the present invention progress；Or not
It is improved, the above-mentioned design of the present invention and technical scheme are directly applied into other occasions, in protection scope of the present invention
Within.
Claims (10)
1. a kind of complex microbial inoculum, include each strain of following percentage by weight：
Bacillus amyloliquefaciens 20~35%, bacillus subtilis 25~40%, bacillus licheniformis 15~30% and colloid bud
Spore bacillus 20~30%.
2. the preparation method of complex microbial inoculum according to claim 1, it is characterised in that the preparation method is to solve
Bacillus amyloliquefacienses, bacillus subtilis, bacillus licheniformis and colloid bacillus cereus are respectively successively by inoculated and cultured, volume production
After culture, according to formula ratio by the strain after each volume production culture after intersecting antagonism culture be made.
3. the preparation method of complex microbial inoculum according to claim 2, it is characterised in that the inoculated and cultured is first to exist
36~48h of inoculated and cultured on solid medium, cultivation temperature are 25~32 DEG C；Afterwards inoculated and cultured 36 on liquid medium within~
48h, cultivation temperature are 28~30 DEG C, and shaking flask speed is 50~70r/min.
4. the preparation method of complex microbial inoculum according to claim 2, it is characterised in that the volume production culture is by liquid
Strain after body medium culture, which is inoculated in fermentation tank, to be cultivated, and the condition of the volume production culture is：Strain inoculum concentration be 3~
10%；Less than 30 DEG C are cultivated 120h, and 5min is stirred per 12h in incubation, the inflation 10min per 2h, after culture, bacterium in zymotic fluid
Number reaches 10~15 × 1010/ml。
5. the preparation method of complex microbial inoculum according to claim 2, it is characterised in that the intersection antagonism culture institute
It is with medium component：20~40g/L of glucose, 30~50g/L of molasses, urea 10~12g/L, P2O55~8g/L, KH2PO4
2~5g/L, remaining is distilled water；PH value 6.8~7.0.
6. the preparation method of complex microbial inoculum according to claim 2, it is characterised in that the intersection antagonism culture
Condition is：Per 4h, stirring 5min, every 2h inflate 10min, co-culture 120h in incubation；In the mixed liquor for intersecting antagonism culture
Viable count is 5~8 × 1010Cfu/ml, as finished product.
7. the system of sewage is administered using complex microbial inoculum described in claim 1 a kind of, including sewage degraded pond and is used for
To the aerator of sewage degraded pond aeration, it is characterised in that the sewage degraded pond includes the multistage overflow drop being sequentially connected
Xie Chi, separated by dividing plate between adjacent overflow degraded pond, and the height of dividing plate imports microbial composite bacteria from sewage degraded pond
The water inlet end of agent reduces successively to water outlet end direction.
8. system according to claim 7, it is characterised in that the bottom face of the overflow degraded pond is from water inlet end to water outlet
The inclined plane structure that extreme direction tilts down.
9. system according to claim 8, it is characterised in that the aerator includes aeration source, appendix and aeration tube,
The aeration source is connected by appendix with aeration tube, and the aeration tube is located at the bottom side of overflow degraded pond introversion ramp structure
On.
10. system according to claim 9, it is characterised in that the aeration source is electromagnetic air pump, the aeration tube
Aeration quantity is 200~300L/min, and aeration time is 8~15min.
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