CN108101707A - A kind of full vegetative microorganism type amino acid water-soluble fertilizer and preparation method thereof - Google Patents
A kind of full vegetative microorganism type amino acid water-soluble fertilizer and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to a kind of full vegetative microorganism type amino acid water-soluble fertilizers and preparation method thereof, the amino acid water-soluble fertilizer includes mixed fermentation liquid and amino acid powder, mixed fermentation liquid by silicate bacteria zymotic fluid and Pediococcus pentosaceus zymotic fluid group into, effective bacterial content of silicate bacteria zymotic fluid is 10,000,000,000/more than g, effective bacterial content of Pediococcus pentosaceus zymotic fluid is 5,000,000,000/more than g, and the weight ratio of silicate bacteria zymotic fluid and Pediococcus pentosaceus zymotic fluid is (1 3):(1 3), amino acid powder weight is 15 times of mixed fermentation liquid weight, and in amino acid powder, glycine accounts for amino acid powder weight percent more than or equal to 23%, and arginine accounts for amino acid powder weight percent less than or equal to 8%.Promotion, output increased and the disease that the full vegetative microorganism type amino acid water-soluble fertilizer of the present invention realizes Crop Stage production reduce.
Description
Technical field
The invention belongs to fertilizer fields, are specifically related to a kind of full vegetative microorganism type amino acid water-soluble fertilizer and its preparation
Method.
Background technology
Nutriment necessary to amino acid is plant growth is structure cell, the basic material of repair tissue.Due to ammonia
The base acid characteristic of itself, as the accelerating agent and catalyst that various enzymes are synthesized in plant, plays plant metabolism important
Effect, it can increase chlorophyll content of plant, improve the activity of enzyme, promote the infiltration of carbon dioxide, make photosynthesis more
Vigorous, to improving crop quality, the content for increasing Vc and sugar suffers from important function.
Research shows that crop has very high absorption, transport and Utilization ability to amino acid water-soluble fertilizer, can supplement rapidly
Itself required nutrient and amino acid, effectively adjust Nutrient Absorption and nutrient accumulation, promote robust plant growth, improve agriculture
Yield, quality and the resistance of product.
The metabolite that microorganism generates in growth course is being repaired and is being improved soil environment, promoting crop root life
Harm that is long, reducing soil-borne disease, the usage amount for reducing chemical pesticide etc. play an important role.As people are to food
Product are increasingly paid attention to safely, therefore, greatly develop high-efficiency low-toxicity, strong functional microorganism correlation research and development of products, reach with bacterium
The effect of diseases prevention reduces chemical pesticide in production and comes into operation, and realizes that agricultural product quality and safety is promoted, agroecological environment is protected
The sustainable development mutually coordinated is protected, while reduces agriculture production cost, promotes peasant's cost-saving synergistic.
At present, China's agricultural is a large amount of unreasonable using chemical fertilizer, and the soil fertility for causing getting worse declines, on pest and disease damage
The modern agriculture diseases such as liter, quality of agricultural product decline, and there is a serious shortage in the supply for water resource, therefore national promotion " water-fertilizer integral " energetically,
Fertilization mode, Developing Water-saving Agriculture are improved, water soluble fertilizer has compared with common chemical fertilizer and reduces nutrient loss, fertilizer utilization
Rate is high, it is water-saving the advantages that.And amino acid water-soluble fertilizer starts to be taken seriously as a kind of new-type fertilizer with notable water-saving function
It progressively promotes, has obtained the accreditation of more and more clay fertilizer experts, agricultural technology extension expert and peasant.
But current market sales of amino acid water-soluble fertilizer, Ministry of Agriculture's NY1429-2010 lowest bids are performed mostly
Standard, micronutrient levels is more than 20g/L or moderate-element content is more than 30g/L, and big, middle and trace element is not added with completely or closes
Reason proportioning, the water-soluble fertilizer of amino acid containing beneficial microorganism is even more fewer and fewer, and crop also needs repeatedly fertilising to supplement other nutrition
Substance not only makes to be difficult to reach absorption equilibrium of mutually promoting between each element, but also increases fertilising cost, and " water-saving " also becomes sky
It talks.
The present invention is according to the above problem, and based on silicate bacteria zymotic fluid and Pediococcus pentosaceus zymotic fluid, dissolving is multiple
Amino acid powder is closed, produces complex microorganism containing amino acid solution, rational proportion adds micro member in a great number of elements, chelating amino acids
Element invents a kind of all nutrition type water-soluble fertilizer containing amino acid, can effectively facilitate crop growth, enhances crop anti-adversity energy,
Crop yield is improved, improves quality of agricultural product.
Silicate bacteria is also known as colloid bacillus cereus, and key property can be decomposited in the mineral such as feldspar, mica
Potassium, silicon can also decomposite the phosphorus in apatite and secrete plant growth substance and a variety of enzymes, to enhance crop to some
The resistance of disease.Potassium in colloid bacillus cereus is after thalline death, separate out, and can be absorbed and used by plants.As
A kind of critical function bacterium in microbial manure can improve soil available nitrogen, phosphorus content, and it is a variety of to improve crop yield and quality etc.
Effect.
Pediococcus pentosaceus belongs to Pediococcus, is widely used in the production of a variety of traditional fermented foods and beverage, commonly uses
In the research of fermented soybean milk etc..Although being applied in agriculture field application also someone, its be used in mixed way with other bacteriums when
Growth or its that antagonism or antagonism other bacteriums often occur are waited by other bacterium antagonisms.
The technical problems to be solved by the invention are to realize that silicate bacteria and Pediococcus pentosaceus can be combined with each other promotion
Be applied to crop fertilizer in.Specifically, providing one kind effectively facilitates crop growth, enhance crop anti-adversity energy, improve
Crop yield improves the full vegetative microorganism type amino acid water-soluble fertilizer of soil texture.
The content of the invention
In order to ensure that silicate bacteria can realize residual organic matter in the decomposition to the ore in soil and soil
It decomposes, using silicate bacteria zymotic fluid and Pediococcus pentosaceus zymotic fluid, but since both zymotic fluids are in decomposable process
The antagonism of part can be generated, that is, there is the production inhibited mutually in the soil, so needing to add in amino acid, and amino
The primary quantity of acid ensures the breeding of bacterium, and the Rapid Fermentation of two kinds of bacterium is realized after initial infestation.
In order to solve the above technical problems, the present invention provides a kind of all nutrition type amino acid water-soluble fertilizer, the amino acid
Water-soluble fertilizer includes mixed fermentation liquid and amino acid powder, and mixed fermentation liquid is fermented by silicate bacteria zymotic fluid and Pediococcus pentosaceus
Liquid forms, and effective bacterial content of silicate bacteria zymotic fluid is 10,000,000,000/more than g, effective bacterial content of Pediococcus pentosaceus zymotic fluid
For 5,000,000,000/more than g, the weight ratio of silicate bacteria zymotic fluid and Pediococcus pentosaceus zymotic fluid is (1-3):(1-3), amino acid powder
Weight is 1-5 times of mixed fermentation liquid weight, and in amino acid powder, glycine accounts for amino acid powder weight percent and is more than or equal to
23%, arginine accounts for amino acid powder weight percent less than or equal to 8%.
Preferably, in above-mentioned all nutrition type amino acid water-soluble fertilizer, in the amino acid powder, glycine accounts for amino acid powder weight
Amount percentage is 23-50%, and arginine accounts for amino acid powder weight percent 1-8%.
Preferably, in above-mentioned all nutrition type amino acid water-soluble fertilizer, also comprising a great number of elements fertilizer, moderate-element fertilizer and micro
Element fertilizer, the wherein weight of a great number of elements fertilizer are 1-6 times of mixed fermentation liquid weight, and the wherein weight of moderate-element fertilizer is mixing
0.3-3 times of zymotic fluid weight, the weight of trace element fertilizer are 0.1-1 times of mixed fermentation liquid weight.
Preferably, in above-mentioned all nutrition type amino acid water-soluble fertilizer, a great number of elements fertilizer by urea, potassium dihydrogen phosphate and
Potassium nitrate forms, and the weight ratio of urea, potassium dihydrogen phosphate and potassium nitrate is (6-15):(6-15):(10-20).
Preferably, in above-mentioned all nutrition type amino acid water-soluble fertilizer, the moderate-element fertilizer includes calcium ammonium nitrate and hydration
One or both of sodium metasilicate (being generally also referred to as instant silicon).
Preferably, in above-mentioned all nutrition type amino acid water-soluble fertilizer, the moderate-element fertilizer is preferably by calcium ammonium nitrate and water
Close sodium metasilicate composition.
Preferably, in above-mentioned all nutrition type amino acid water-soluble fertilizer, the trace element fertilizer includes boric acid, zinc nitrate and sulphur
One or more in sour ferrous iron.
Preferably, in above-mentioned all nutrition type amino acid water-soluble fertilizer, the trace element fertilizer is by boric acid, zinc nitrate and sulfuric acid
Ferrous iron composition.
Preferably, in above-mentioned all nutrition type amino acid water-soluble fertilizer, the full vegetative microorganism type amino acid water-soluble fertilizer
Comprising 4-12 parts of silicate bacteria zymotic fluid, 4-12 parts of Pediococcus pentosaceus zymotic fluid, 24-35 parts of amino acid powder, 6-15 parts of urea,
6-15 parts of potassium dihydrogen phosphate, 10-20 parts of potassium nitrate, 8-20 parts of moderate-element, micro- 2-8 parts and 50-70 parts of water, wherein silicon
Effective bacterial content of hydrochlorate ferment product is more than 10,000,000,000/g；Effective bacterial content of Pediococcus pentosaceus zymotic fluid is more than 5,000,000,000/g,
In amino acid powder, it is 25% that glycine, which accounts for amino acid powder weight percent, and arginine accounts for amino acid powder weight percent as 3%.
Preferably, in above-mentioned all nutrition type amino acid water-soluble fertilizer, the moderate-element fertilizer includes calcium ammonium nitrate and hydration
One or both of sodium metasilicate.
Preferably, in above-mentioned all nutrition type amino acid water-soluble fertilizer, the trace element fertilizer includes boric acid, zinc nitrate and sulphur
One or more in sour ferrous iron.
The present invention also provides the preparation methods of above-mentioned all nutrition type amino acid water-soluble fertilizer, and the described method includes following steps
Suddenly：
Step 1：The preparation of silicate bacteria zymotic fluid
Step 2：The preparation of Pediococcus pentosaceus zymotic fluid
Step 3：Complex microorganism is prepared containing amino acid solution
25-35 parts of amino acid powders are dissolved in 50-70 parts of water, by 4-12 portions of silicate bacteria zymotic fluids and 4-12 parts of pentoses
Piece coccus zymotic fluid adds in, and obtains complex microorganism containing amino acid solution.
Step 4：A great number of elements adds
Contain to complex microorganism in amino acid solution and sequentially add 6-15 parts of urea, 6-15 parts of potassium dihydrogen phosphate, potassium nitrate
It 10-20 parts, stirs to being completely dissolved, obtains the complex microorganism containing a great number of elements containing amino acid solution.
Step 5：Moderate-element addition chelating
To the above-mentioned complex microorganism containing a great number of elements containing 6-14 parts of calcium ammonium nitrates of addition, 2-6 parts of speed in amino acid solution
Molten silicon, adjustment pH value stir to being completely dissolved between 5~6, being heated to 40 DEG C or so, obtain answering containing a large amount of moderate-elements
It closes microorganism and contains amino acid solution.
Step 6：Trace element addition chelating
To the above-mentioned complex microorganism containing a large amount of moderate-elements containing 1-4 parts of boric acid of addition, 0.5-2 parts of nitre in amino acid solution
Sour zinc, 0.5-2 parts of ferrous sulfate, adjustment pH value are stirred to being completely dissolved, obtained between 5.5~6.5, being heated to 40 DEG C or so
To full vegetative microorganism type amino acid water-soluble fertilizer.
The present invention also provides the purposes of above-mentioned full vegetative microorganism type amino acid water-soluble fertilizer, the purposes is raising crop
The purposes of solvable inorganic constituents and SOF Soluble Organic Fraction content in yield, crop resistance and raising soil.
Beneficial effects of the present invention：
The full vegetative microorganism type amino acid water-soluble fertilizer of the present invention, by by silicate bacteria zymotic fluid and pentose piece ball
The mixed fermentation liquid of fermented liquid composition decomposes the level decomposed with organic matter to improve inorganic matter in soil, while solves silicic acid
The initial stage antagonism function of salt bacterium and Pediococcus pentosaceus during the fermentation, that is, the battalion of two kinds of bacterium of initial stage during the fermentation
Form be divided to contention or nutrient environment be not suitable for and cause the expression activitiy of two kinds of bacterium low.The present invention is by adding in specific amino
Sour powder solves the problems, such as this, realizes and is independent of each other on the activation plays of two kinds of bacterium in the early stage, the sheet for realizing two kinds of bacterium is active
Energy.
The full vegetative microorganism type amino acid water-soluble fertilizer of the present invention realizes the promotion of Crop Stage production, output increased
It is reduced with disease.
The full vegetative microorganism type amino acid water-soluble fertilizer of the present invention realizes the drop to inorganic matter in soil and organic matter
Solution so that the raising of the soluble inorganic matter content of soil, the content of dissolved organic matter improve, and are the soil ring of plant growth
The improvement in border has excellent effect.
Specific embodiment
In example below, in order to verify silicate bacteria and Pediococcus pentosaceus and different amino in the Water soluble fertilizer of the present invention
The effect of sour powder cooperation especially verifies the influence that the performance to two kinds of zymotic fluids of glycine and arginine content plays, special
Different content glycine and arginic amino acid powder are prepared, prepares four kinds of amino acid powders A, B, C and D respectively, amino acid powder E is
The original of the fish meal amino acid powder of purchase.
By adjusting thuja acid ammonium and arginic content to amino acid coarse powder, during preparing amino acid, arginine content
It is diluted in the case of height with glycine, glycine is added in the case that Glycine Levels are low.It is Glycine Levels under normal circumstances
Deficiency, arginine content are generally also required to the arginine sterling that addition is bought if necessary to adjust to more than 3%.
The code name and glycine of specific amino acid powder and arginic content situation are as follows：
Amino acid powder A：Glycine 25%, arginine 3%
Amino acid powder B：Thuja acid ammonium 23%, arginine 8%
Amino acid powder C：Glycine 15%, arginine 10%
Amino acid powder D：Glycine 30%, arginine 15%
Amino acid powder E：Glycine 4.52%, arginase 12 .18%
In above-mentioned amino acid powder, amino acid powder E is the fish meal amino acid powder of purchase, wherein glycine and arginic content
For actually detected value.Amino acid powder A-D is the influence of verification glycine and arginine to the effect of Water soluble fertilizer.
The strain of silicate bacteria and Pediococcus pentosaceus is market purchase, for conventional silicate bacteria and pentose piece ball
Bacterium.
Embodiment 1：The preparation of full vegetative microorganism type amino acid water-soluble fertilizer I
Step 1：The preparation of silicate bacteria zymotic fluid
With the one cyclosilicate microbionation of oese picking into seed culture medium slant tube, activated at 37 DEG C of temperature
18h；It is inoculated into two ring activated silicates bacteria culture of oese picking during the 500ml equipped with 150ml Shake flask mediums shakes,
It is cultivated for 24 hours under conditions of 37 DEG C of temperature and rotating speed 120r/min in constant-temperature table, it is spare that seed liquor is made；According to fermentation tank
The inoculum concentration of culture medium weight meter 5%, diastatochromogenes is inoculated into fermentation tank culture medium, in 35~37 DEG C of temperature, ventilation
Amount 1:1st, ferment under conditions of tank pressure 0.05MPa and rotating speed 120r/min；The 12h that ferments starts sampling detection, and every two is small
When sampling once, sediments microscope inspection treats that spore forming rate reaches more than 90%, effective bacterial content in bacterium solution for 10,000,000,000/g with
Terminated to ferment when upper, obtain silicate bacteria zymotic fluid.
Seed culture medium is prepared according to following preparation methods：
By 5 parts by weight of sucrose, 2 parts by weight of phosphoric acid potassium dihydrogens, 0.5 parts by weight epsom salt, 0.5 parts by weight lightweight carbonic acid
Calcium and 15 parts by weight agar, are dissolved in 1000 parts by weight distilled water, are uniformly mixed, and then sterilize 30min at 121 DEG C of temperature,
Obtain the seed culture medium；
Shake flask medium is prepared according to following preparation methods：
10 parts by weight of sucrose, 2 parts by weight of phosphoric acid potassium dihydrogens, 0.5 parts by weight epsom salt, 0.2 parts by wt NaCl, 1
Parts by weight precipitated calcium carbonate is dissolved in 1000 parts by weight distilled water, is uniformly mixed, and then sterilize 30min at 121 DEG C of temperature,
Obtain the Shake flask medium；
Fermentation tank culture medium is prepared according to following preparation methods：
3 parts by weight of sucrose, 10 parts by weight glucose, 12 weight starch, 2 parts by weight epsom salts, 2 parts by weight of phosphoric acid
Hydrogen dipotassium, 2 parts sulfuric acid ammoniums, 2 parts by weight precipitated calcium carbonates, 0.1 parts by weight iron chloride, are dissolved in 1000 parts by weight distilled water
In, it is uniformly mixed, then sterilize 30min at 121 DEG C of temperature, obtains the fermentation tank culture medium.
Step 2：The preparation of Pediococcus pentosaceus zymotic fluid
It is inoculated into one ring Pediococcus pentosaceus strain of oese picking in seed culture medium slant tube, at 37 DEG C of temperature
Activate 18h；It is inoculated into two ring of oese picking activation Pediococcus pentosaceus strain equipped with 150ml Shake flask medium 500ml shaking flasks
In, it is cultivated for 24 hours under conditions of 37 DEG C of temperature and rotating speed 120r/min in constant-temperature table, it is spare that seed liquor is made；According to
The inoculum concentration of fermentation tank culture medium weight meter 3%, diastatochromogenes is inoculated into fermentation tank culture medium, in 35-37 DEG C, tank pressure
0.04mpa, rotating speed 200rpm/min fermented and cultureds.Effective bacterial content in bacterium solution terminates fermentation when being 5,000,000,000/more than g and obtains penta
Sugar-tablet coccus zymotic fluid.
Seed culture medium is prepared according to following preparation methods：
By 2 parts by weight glucose, 0.5 parts by weight dusty yeast, 1 parts by weight beef extract, 1 parts by weight peptone, 0.05 weight
Part magnesium sulfate, 0.005 parts sulfuric acid manganese, are dissolved in 1000 parts by weight distilled water, are uniformly mixed, then at 121 DEG C of temperature
Sterilize 30min, obtains the seed culture medium.
Shake flask medium is prepared according to following preparation methods：
By 2 parts by weight glucose, 0.5 parts by weight dusty yeast, 1 parts by weight beef extract, 1 parts by weight peptone, 0.5 parts by weight
Sodium acetate, 0.1 parts by weight of phosphoric acid potassium dihydrogen, 0.2 parts by weight of lemon acid hydrogen diammonium, 0.052 parts sulfuric acid magnesium, 0.005 weight
Part manganese sulfate, Tween 80 1ml, 1 parts by weight precipitated calcium carbonate, are dissolved in 1000 parts by weight distilled water, are uniformly mixed, then in temperature
Sterilize 30min at 121 DEG C of degree, obtains the Shake flask medium.
Fermentation tank culture medium is prepared according to following preparation methods：
3.5 parts by weight glucose, 1.0 parts by weight dusty yeasts, 1.0 parts by weight beef extracts, 0.6 parts by weight sodium acetate, 0.2 weight
Measure part potassium dihydrogen phosphate, 0.2 parts by weight of lemon acid hydrogen diammonium, 0.05 parts sulfuric acid magnesium, 0.006 parts sulfuric acid manganese, 1 weight
Part precipitated calcium carbonate, Tween 80 1.5ml, are dissolved in 1000 parts by weight distilled water, are uniformly mixed, and then go out at 121 DEG C of temperature
Bacterium 30min obtains the fermentation tank culture medium.
Step 3：Complex microorganism is prepared containing amino acid solution
30 parts of amino acid powder A are dissolved in 60 parts of water, by 8 portions of silicate bacteria zymotic fluids and 8 parts of Pediococcus pentosaceus fermentations
Liquid adds in, and obtains complex microorganism containing amino acid solution.
Step 4：A great number of elements adds
Contain to complex microorganism in amino acid solution and sequentially add 10 parts of urea, 10 parts of potassium dihydrogen phosphate, 15 parts of potassium nitrate,
Stirring obtains the complex microorganism containing a great number of elements containing amino acid solution to being completely dissolved.
Step 5：Moderate-element addition chelating
Contain to the above-mentioned complex microorganism containing a great number of elements and 10 parts of calcium ammonium nitrates, 4 parts of instant silicon added in amino acid solution,
PH value is adjusted between 5~6, being heated to 40 DEG C or so, stirs to being completely dissolved, obtains the composite microbial containing a large amount of moderate-elements
Object contains amino acid solution.
Step 6：Trace element addition chelating
Contain to the above-mentioned complex microorganism containing a large amount of moderate-elements and 3 parts of boric acid, 1 part of zinc nitrate are added in amino acid solution, 1
Part ferrous sulfate between adjustment pH value to 5.5~6.5, is heated to 40 DEG C or so, stirs to being completely dissolved, it is micro- to obtain full nutrition
Bion amino acid water-soluble fertilizer I.
Embodiment 2：The preparation of full vegetative microorganism type amino acid water-soluble fertilizer II
According to the method for embodiment 1, amino acid powder A is replaced with amino acid powder B, remaining preparation condition is constant, is sought entirely
Support microbiocidal amino acid water-soluble fertilizer II.
Embodiment 3：The preparation of full vegetative microorganism type amino acid water-soluble fertilizer III
According to the method for embodiment 1, amino acid powder A is replaced with amino acid powder C, remaining preparation condition is constant, is sought entirely
Support microbiocidal amino acid water-soluble fertilizer III.
Embodiment 4：The preparation of full vegetative microorganism type amino acid water-soluble fertilizer IV
According to the method for embodiment 1, amino acid powder A is replaced with amino acid powder D, remaining preparation condition is constant, is sought entirely
Support microbiocidal amino acid water-soluble fertilizer IV.
Embodiment 5：The preparation of full vegetative microorganism type amino acid water-soluble fertilizer V
According to the method for embodiment 1, amino acid powder A is replaced with amino acid powder D, remaining preparation condition is constant, is sought entirely
Support microbiocidal amino acid water-soluble fertilizer V.
Embodiment 6：The preparation of full vegetative microorganism type amino acid water-soluble fertilizer VI
According to the method for embodiment 1, amino acid powder A is replaced with 30 parts of distilled water, remaining preparation condition is constant, is sought entirely
Support microbiocidal amino acid water-soluble fertilizer VI.
The full vegetative microorganism type amino acid water-soluble fertilizer I-VI being prepared, according to NY884-2012《Biological organic fertilizer》
Standard method of analysis has carried out analysis and has measured, the result shows that, its total living bacteria count >=5.4 hundred million/g；The content of organic matter is
By weight >=15.4%；According to NY1107-2010《A great number of elements water-soluble fertilizer》Standard method of analysis has carried out analysis and has measured,
N P and K >=20.8%；According to NY1429-2010《Water-soluble fertilizer containing amino acid》Standard method of analysis has carried out analysis and has measured, calcium
Silicone content >=3.5%；Boron zinc-iron >=0.5%.
Test example 1：The wheat application test of full vegetative microorganism type amino acid water-soluble fertilizer
The embodiment of the test example is as follows：
Test variety：Wheat, all wheats 22.
Test period：On June 30,2 days to 2016 October in 2015.
Test site：Henan Province Jiaozuo City Xiuwu County Zhouzhuang township Meng Cun.
Test method：Experiment sets 8 processing, and each processing is repeated 3 times, and sets 24 cells, plot area 30m altogether2.Examination
It tests top dressing and other management measures is identical.
Processing 1：400 times of liquid of the full vegetative microorganism type amino acid water-soluble fertilizer I of conventional fertilizer application+spray, jointing stage, booting
Phase and pustulation period respectively spray once；
Processing 2：400 times of liquid of the full vegetative microorganism type amino acid water-soluble fertilizer II of conventional fertilizer application+spray, it is the jointing stage, pregnant
Ear period and pustulation period respectively spray once；
Processing 3：400 times of liquid of the full vegetative microorganism type amino acid water-soluble fertilizer III of conventional fertilizer application+spray, it is the jointing stage, pregnant
Ear period and pustulation period respectively spray once；
Processing 4：400 times of liquid of the full vegetative microorganism type amino acid water-soluble fertilizer IV of conventional fertilizer application+spray, it is the jointing stage, pregnant
Ear period and pustulation period respectively spray once；
Processing 5：400 times of liquid of the full vegetative microorganism type amino acid water-soluble fertilizer V of conventional fertilizer application+spray, jointing stage, booting
Phase and pustulation period respectively spray once；
Processing 6：400 times of liquid of the full vegetative microorganism type amino acid water-soluble fertilizer VI of conventional fertilizer application+spray, it is the jointing stage, pregnant
Ear period and pustulation period respectively spray once；
Processing 7：Conventional fertilizer application+and 1 same period of processing spray commercially available Amino acid liquid fertilizer；
Processing 8：Conventional fertilizer application+and 1 same period of processing spray equivalent clear water；
The experiment carries out on the basis of conventional fertilizer application.Conventional fertilizer application is：Mu bottom application 25-12-8 composite fertilizer 50kg, jointing stage
Mu imposes urea 15kg.It is sprayed entirely respectively on March 30th, 2017, April 18, May 8 in strict accordance with the requirement of this testing program
Vegetative microorganism type amino acid water-soluble fertilizer, commercially available Amino acid liquid fertilizer and clear water.Each cell list receives single title meter production during harvest,
And it is carried out at the same time field investigation and species test.In addition to punching fertilising liquid or clear water is required according to this programme, the other management measures of the experiment
It is identical with general field production management.
1st, to the influence of wheat growth stage
Wheat growth stage investigation is shown in Table 1, and by following table, it can be seen that spraying the present invention, vegetative microorganism type amino acid is water-soluble entirely
Fertilizer has wheat growth stage certain facilitation, is mainly manifested in the jointing stage, heading stage, maturity period index have different journeys
Degree improves.
1 wheat growth stage application form of table
| Breeding time | Processing 1 | Processing 2 | Processing 3 | Processing 4 | Processing 5 | Processing 6 | Processing 7 | Processing 8 |
| Sowing time | 16/10 | 16/10 | 16/10 | 16/10 | 16/10 | 16/10 | 16/10 | 16/10 |
| Seeding stage | 24/10 | 24/10 | 24/10 | 24/10 | 24/10 | 24/10 | 24/10 | 24/10 |
| Tillering stage | 22/11 | 22/11 | 22/11 | 22/11 | 22/11 | 22/11 | 22/11 | 22/11 |
| Jointing stage | 28/3 | 28/3 | 28/3 | 28/3 | 28/3 | 29/3 | 29/3 | 30/3 |
| Heading stage | 19/4 | 19/4 | 19/4 | 19/4 | 20/4 | 21/4 | 23/4 | 25/4 |
| Maturity period | 4/6 | 5/6 | 6/6 | 6/6 | 7/6 | 8/6 | 12/6 | 16/6 |
From the point of view of the morning and evening in each growth period, the full vegetative microorganism type amino acid water-soluble fertilizer of Application Example 1 can carry
The time in preceding maturity period is most long, and the full vegetative microorganism type amino acid water-soluble fertilizer energy of application the embodiment of the present invention 1-5
At least 5 days enough will be shifted to an earlier date at heading stage.
2nd, to the influence of Important Diseases in Wheat
2 Important Diseases in Wheat of table is investigated
Wheat sharp eyespot and powdery mildew are the Major Diseases on China's wheat, become influence Wheat Production an important factor for.
Each processing is investigated after check plot is generally fallen ill, wheat sharp eyespot and powdery mildew investigation are shown in Table 2, can be seen by upper table
Go out to spray the full vegetative microorganism type amino acid water-soluble fertilizer of the embodiment of the present invention 1, to wheat sharp eyespot and powdery mildew preventive effect
Up to up to 42.69%, 57.47%, illustrating respectively for expansion of the complex microorganism bacterium solution contained in examination fertilizer to Important Diseases in Wheat
Exhibition sprawling plays good inhibiting effect.
3rd, to wheat into production factor influence
Spraying full vegetative microorganism type amino acid water-soluble fertilizer of the invention improves wheat into production factor.It can from table 3
Go out：Compared with handling 2-8, grain number per spike increases for processing 1, and mass of 1000 kernel increase causes per mu yield to increase.Illustrate the implementation for spraying the present invention
The full vegetative microorganism type amino acid water-soluble fertilizer of example 1 can increase grain number per spike and the mass of 1000 kernel of wheat, while increase per mu yield, real
Now estimate that per mu yield volume increase ratio has reached 19%, this is for the production of wheat almost in miracle.
3 field investigation of table and species test statistical form
4th, to the improvement of wheat soil
Pass through the measurement of the solvable inorganic matter of unit Soluble Organic Matter content and unit to soil, primary Calculation, using reality
Apply the processing 1 of the full vegetative microorganism type amino acid water-soluble fertilizer of example 1, it is higher than handling the content of 2-8, the maturity period into
The measurement of the gross dry weight of Soluble Organic Matter and solvable inorganic matter in row soil finds that processing 1 is solvable more organic than the soil of processing 2
The gross dry weight of matter and solvable inorganic matter improves 4.32%, than the soil Soluble Organic Matter of processing 8 and the gross dry weight of solvable inorganic matter
Improve 12.98%.It can be seen that the present invention by the glycine in amino acid powder and arginic content adjustment play it is unexpected
Soil improvement action.
Claims (10)
1. a kind of all nutrition type amino acid water-soluble fertilizer, the amino acid water-soluble fertilizer includes mixed fermentation liquid and amino acid powder,
Mixed fermentation liquid is by silicate bacteria zymotic fluid with Pediococcus pentosaceus zymotic fluid group into effective bacterium of silicate bacteria zymotic fluid contains
It measures as 10,000,000,000/more than g, effective bacterial content of Pediococcus pentosaceus zymotic fluid is 5,000,000,000/more than g, silicate bacteria zymotic fluid and penta
The weight ratio of sugar-tablet coccus zymotic fluid is (1-3):(1-3), amino acid powder weight are 1-5 times of mixed fermentation liquid weight, amino
In sour powder, glycine accounts for amino acid powder weight percent more than or equal to 23%, and arginine accounts for amino acid powder weight percent and is less than etc.
In 8%.
2. all nutrition type amino acid water-soluble fertilizer according to claim 1, it is characterised in that in the amino acid powder, sweet ammonia
Acid accounts for amino acid powder weight percent as 23-50%, and arginine accounts for amino acid powder weight percent 1-8%.
3. all nutrition type amino acid water-soluble fertilizer according to claim 1, it is characterised in that all nutrition type amino acid
Water-soluble fertilizer also comprising a great number of elements fertilizer, moderate-element fertilizer and trace element fertilizer, for mixing send out by the wherein weight of a great number of elements fertilizer
The weight of 1-6 times of zymotic fluid weight, wherein moderate-element fertilizer is 0.3-3 times of mixed fermentation liquid weight, the weight of trace element fertilizer
It measures as 0.1-1 times of mixed fermentation liquid weight.
4. all nutrition type amino acid water-soluble fertilizer according to claim 1, it is characterised in that a great number of elements fertilizer is by urinating
Element, potassium dihydrogen phosphate and potassium nitrate composition, the weight ratio of urea, potassium dihydrogen phosphate and potassium nitrate is (6-15):(6-15):(10-
20)。
5. all nutrition type amino acid water-soluble fertilizer according to claim 1, it is characterised in that the moderate-element fertilizer includes
Calcium ammonium nitrate and hydrated sodium silicate（Generally it is also referred to as instant silicon）One or both of.
6. all nutrition type amino acid water-soluble fertilizer according to claim 1, it is characterised in that the trace element fertilizer includes
One or more in boric acid, zinc nitrate and ferrous sulfate.
A kind of 7. all nutrition type amino acid water-soluble fertilizer, it is characterised in that the full vegetative microorganism type amino acid water-soluble fertilizer bag
4-12 parts of silicate ferment product, 4-12 parts of Pediococcus pentosaceus zymotic fluid, 24-35 parts of amino acid powder, 6-15 parts of urea, phosphorus
6-15 parts of acid dihydride potassium, 10-20 parts of potassium nitrate, 8-20 parts of moderate-element, micro- 2-8 parts and 50-70 parts of water, wherein silicic acid
Effective bacterial content of salt ferment product is more than 10,000,000,000/g；Effective bacterial content of Pediococcus pentosaceus zymotic fluid is more than 5,000,000,000/g, ammonia
In base acid powder, it is 25% that glycine, which accounts for amino acid powder weight percent, and arginine accounts for amino acid powder weight percent as 3%.
8. all nutrition type amino acid water-soluble fertilizer according to claim 7, it is characterised in that the moderate-element fertilizer includes
One or both of calcium ammonium nitrate and hydrated sodium silicate, the trace element fertilizer are included in boric acid, zinc nitrate and ferrous sulfate
One or more.
9. the preparation method of all nutrition type amino acid water-soluble fertilizer described in claim 7 or 8, the described method includes following steps
Suddenly：
Step 1：The preparation of silicate bacteria zymotic fluid；
Step 2：The preparation of Pediococcus pentosaceus zymotic fluid；
Step 3：Complex microorganism is prepared containing amino acid solution：
25-35 parts of amino acid powders are dissolved in 50-70 parts of water, by 4-12 portions of silicate bacteria zymotic fluids and 4-12 parts of pentose piece balls
Fermented liquid adds in, and obtains complex microorganism containing amino acid solution；
Step 4：A great number of elements adds：
Contain to complex microorganism in amino acid solution and sequentially add 6-15 parts of urea, 6-15 parts of potassium dihydrogen phosphate, potassium nitrate 10-20
Part, it stirs to being completely dissolved, obtains the complex microorganism containing a great number of elements containing amino acid solution；
Step 5：Moderate-element addition chelating：
Contain to the above-mentioned complex microorganism containing a great number of elements and 6-14 parts of calcium ammonium nitrates, 2-6 parts of instant silicon added in amino acid solution,
PH value is adjusted between 5~6, being heated to 40 DEG C or so, stirs to being completely dissolved, obtains the composite microbial containing a large amount of moderate-elements
Object contains amino acid solution；
Step 6：Trace element addition chelating：
To the above-mentioned complex microorganism containing a large amount of moderate-elements containing 1-4 parts of boric acid of addition, 0.5-2 parts of nitric acid in amino acid solution
Zinc, 0.5-2 parts of ferrous sulfate, adjustment pH value are stirred to being completely dissolved, obtained between 5.5~6.5, being heated to 40 DEG C or so
Full vegetative microorganism type amino acid water-soluble fertilizer.
10. the purposes of the full vegetative microorganism type amino acid water-soluble fertilizer described in claim 7 or 8, the purposes is made to improve
The purposes of solvable inorganic constituents and SOF Soluble Organic Fraction content in produce amount, crop resistance and raising soil.
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| CN111875432A (en) * | 2020-06-28 | 2020-11-03 | 张德宏 | Amino acid-containing water-soluble fertilizer |
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