CN112552103A - Microbial agent containing biochemical fulvic acid potassium and preparation method thereof - Google Patents
Microbial agent containing biochemical fulvic acid potassium and preparation method thereof Download PDFInfo
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- CN112552103A CN112552103A CN202011380844.5A CN202011380844A CN112552103A CN 112552103 A CN112552103 A CN 112552103A CN 202011380844 A CN202011380844 A CN 202011380844A CN 112552103 A CN112552103 A CN 112552103A
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- microbial agent
- spore liquid
- potassium fulvate
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
Abstract
The invention discloses a microbial agent containing biochemical potassium fulvate and a preparation method thereof, and the microbial agent containing biochemical potassium fulvate comprises the following raw materials in parts by weight: 650-950 parts of fulvic acid potassium, 50 parts of microbial agent spore liquid, 10-200 parts of water, 10-20 parts of industrial monoammonium phosphate, 10-70 parts of diatomite and 10-40 parts of coconut oil acid polyglycerol ester; the strain in the microbial agent spore liquid is bacillus amyloliquefaciens, bacillus subtilis, bacillus licheniformis and bacillus mucilaginosus spore liquid which are compounded according to the proportion of 1:1:1:1, and after the microbial agent is applied, the nutrient absorption capacity of crops can be improved, the stress resistance of plants can be enhanced, and the microbial agent has the effects of promoting growth and improving yield and quality.
Description
Technical Field
The invention relates to the field of microorganisms, in particular to a microbial agent containing biochemical potassium fulvate and a preparation method thereof.
Background
Biochemical potassium fulvate (concentrated molasses liquid) is a viscous liquid obtained by biological fermentation of plant residues as raw materials by applying modern biotechnology.
The potassium fulvate is a pure natural mineral active potassium element fertilizer, contains multiple nutrient components such as trace elements, rare earth elements, plant growth regulators, virus inhibitors and the like, so that the nutrients are more sufficient and more reasonable to supply, various physiological diseases of crops caused by the lack of elements are avoided, the plant types of the crops are more vigorous, the leaf color is darker and greener, and the lodging resistance is stronger. The potassium fulvate can supplement nutrients lost in soil in time, so that the soil is activated, the vitality is realized, the continuous cropping disease caused by excessive absorption of nutrients in the soil is reduced, the product can completely replace potassium sulfate or potassium chloride and potassium magnesium sulfate with the same content, and the product is natural and environment-friendly.
The existing potassium fulvate fertilizer is mostly prepared by mixing with organic matters, and although the existing potassium fulvate fertilizer is organic and environment-friendly, the fertilizer efficiency is poor.
Disclosure of Invention
The invention aims to provide a microbial agent containing biochemical fulvic acid potassium and a preparation method thereof aiming at the defects of the prior art.
In order to achieve the above object, the present invention is achieved by the following technical solutions.
A microbial agent containing biochemical fulvic acid potassium comprises the following raw materials in parts by weight: 650-950 parts of fulvic acid potassium, 50 parts of microbial agent spore liquid, 10-200 parts of water, 10-20 parts of industrial monoammonium phosphate, 10-70 parts of diatomite and 10-40 parts of coconut oil acid polyglycerol ester;
the bacterial strains in the microbial agent spore liquid are bacillus amyloliquefaciens, bacillus subtilis, bacillus licheniformis and bacillus mucilaginosus spore liquid which are compounded according to the mass ratio of 1:1:1:1,
the preservation number of the bacillus amyloliquefaciens is CGMCC No. 20147;
the preservation number of the bacillus subtilis is CGMCC No. 20145;
the preservation number of the bacillus licheniformis is CGMCC No. 20150;
the preservation number of the bacillus mucilaginosus is CGMCC No. 20152.
Further, the feed comprises the following raw materials in parts by weight: 650 parts of potassium fulvate, 50 parts of microbial agent spore liquid, 10 parts of water, 10 parts of industrial monoammonium phosphate, 10 parts of diatomite and 10 parts of coconut oil acid polyglycerol ester.
Further, the feed comprises the following raw materials in parts by weight: 950 parts of potassium fulvate, 50 parts of microbial agent spore liquid, 200 parts of water, 20 parts of industrial monoammonium phosphate, 70 parts of diatomite and 40 parts of coconut oil acid polyglycerol ester.
Further, the feed comprises the following raw materials in parts by weight: 800 parts of potassium fulvate, 50 parts of microbial agent spore liquid, 100 parts of water, 15 parts of industrial monoammonium phosphate, 45 parts of diatomite and 25 parts of coconut oil acid polyglycerol ester.
Further, the feed comprises the following raw materials in parts by weight: 750 parts of potassium fulvate, 50 parts of microbial agent spore liquid, 80 parts of water, 12 parts of industrial monoammonium phosphate, 13 parts of diatomite and 18 parts of coconut oil acid polyglycerol ester.
Further, the feed comprises the following raw materials in parts by weight: 850 parts of potassium fulvate, 50 parts of microbial agent spore liquid, 12 parts of water, 18 parts of industrial monoammonium phosphate, 56 parts of diatomite and 31 parts of coconut oil acid polyglycerol ester.
Furthermore, the number of effective viable bacteria in the spore liquid of the microbial inoculum is more than or equal to 100 hundred million/g.
The preparation method comprises the following steps:
firstly, preparing raw materials: weighing potassium fulvate, microbial agent spore liquid, water, industrial monoammonium phosphate, diatomite and coconut oil acid polyglycerol ester for later use;
secondly, mixing materials: according to the weight parts, 650-950 parts of liquid fulvic acid potassium, 10-200 parts of water, 10-20 parts of industrial monoammonium phosphate, 10-70 parts of diatomite and 10-40 parts of coconut oil acid polyglycerol ester are stirred and mixed uniformly, the industrial monoammonium phosphate is heated, dissolved and mixed in the material, 50 parts of microbial agent spore liquid is added into the material, the mixture is stirred uniformly again, and the mixture is sieved by a 80-mesh sieve for later use after being mixed uniformly;
and step three, putting the material mixed liquor obtained by sieving in the step two into a centrifugal spray dryer, carrying out spray drying, and collecting powder to obtain the microbial agent containing the biochemical fulvic acid potassium.
The invention has the advantages that:
1. compared with the prior art, the invention adopts a compound mixing mode, and has simple working procedures;
2. the raw materials of the invention are all powder, and are mixed to be the full water-soluble microbial inoculum;
3. after the microbial agent added in the invention is applied, the nutrient absorption capacity of crops can be improved, the stress resistance of plants is enhanced, and the microbial agent has the effects of promoting growth and improving yield and quality.
4. The product of the invention is full water-soluble powder, is convenient for transportation and application, can be applied by means of watering, sprinkling irrigation, drip irrigation, foliage spraying and the like, is convenient for use, and saves labor cost; the biochemical potassium fulvate is easy to be absorbed by crops, has the effects of promoting growth, enhancing stress resistance and the like, and can improve the yield and quality of the crops.
Detailed Description
The present invention is further illustrated by the following examples, but is not limited to the details of the description.
A microbial agent containing biochemical fulvic acid potassium comprises the following raw materials in parts by weight: 650-950 parts of fulvic acid potassium (concentrated molasses liquid), 50 parts of microbial agent spore liquid (the effective viable count is more than or equal to 100 hundred million/g), 10-200 parts of water, 10-20 parts of industrial monoammonium phosphate, 10-70 parts of diatomite and 10-40 parts of coconut oil acid polyglycerol ester.
The microbial agent spore liquid strain is prepared by compounding bacillus amyloliquefaciens with the preservation number of CGMCC No.20147, bacillus subtilis with the preservation number of CGMCC No.20145, bacillus licheniformis with the preservation number of CGMCC No.20150 and bacillus mucilaginosus with the preservation number of CGMCC No.20152 according to the proportion of 1:1:1, and the effective viable count is more than or equal to 100 hundred million/g.
Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) with the preservation number of CGMCC No.20147 and the preservation date: 28/6/2020, depository: china general microbiological culture Collection center; and (4) storage address: xilu No. 1 Hospital No. 3, Beijing, Chaoyang, North.
Bacillus subtilis with the preservation number of CGMCC No.20145 and the preservation date: 28/6/2020, depository: china general microbiological culture Collection center; and (4) storage address: xilu No. 1 Hospital No. 3, Beijing, Chaoyang, North.
The preservation number of the Bacillus licheniformis (Bacillus licheniformis) is CGMCC No. 20150; the preservation date is as follows: 28/6/2020, depository: china general microbiological culture Collection center; and (4) storage address: xilu No. 1 Hospital No. 3, Beijing, Chaoyang, North.
The preservation number of the Bacillus mucilaginosus (Bacillus mucoarginosus) is CGMCC No.20152, and the preservation date is as follows: 28/6/2020, depository: china general microbiological culture Collection center; and (4) storage address: xilu No. 1 Hospital No. 3, Beijing, Chaoyang, North.
The preparation method comprises the following steps:
(1) preparing raw materials: liquid biochemical potassium fulvate (concentrated molasses liquid), microbial agent spore liquid (the number of effective viable bacteria is more than or equal to 100 hundred million/g), water, industrial monoammonium phosphate, diatomite as an adsorbent, and coconut oil acid polyglycerol ester to reduce the viscosity of raw materials.
(2) Material mixing: according to the weight portion, 650 weight portions of liquid biochemical fulvic acid potassium (concentrated molasses liquid), 50 weight portions of microbial agent spore liquid (the number of effective viable bacteria is more than or equal to 100 hundred million/g), 10 to 200 weight portions of water, 10 to 20 weight portions of industrial monoammonium phosphate, 10 to 70 weight portions of diatomite and 10 to 40 weight portions of coconut oil acid polyglycerol ester are taken, wherein the industrial monoammonium phosphate needs to be heated, dissolved and then mixed in the materials, 50 weight portions of microbial agent spore liquid (the number of effective viable bacteria is more than or equal to 100 hundred million/g) are added into the materials and stirred uniformly again, and all the materials are sieved by a sieve of 80 meshes for standby after being uniformly mixed.
(3) And (3) putting the material mixed liquor obtained in the step (2) into a high-speed centrifugal spray dryer, carrying out spray drying, and collecting powder to obtain the microbial agent containing the biochemical fulvic acid potassium. The high speed centrifugal spray dryer was purchased from santai drying equipment ltd, changzhou.
Detailed description of the preferred embodiment 1
A microbial agent containing biochemical fulvic acid potassium comprises the following raw materials in parts by weight: 650 parts of fulvic acid potassium (concentrated molasses liquid), 50 parts of microbial agent spore liquid (the effective viable count is more than or equal to 100 hundred million/g), 10 parts of water, 10 parts of industrial monoammonium phosphate, 10 parts of diatomite and 10 parts of coconut oil acid polyglycerol ester.
Detailed description of the preferred embodiment 2
A microbial agent containing biochemical fulvic acid potassium comprises the following raw materials in parts by weight: 950 parts of potassium fulvate (concentrated molasses liquid), 50 parts of microbial agent spore liquid (the effective viable count is more than or equal to 100 hundred million/g), 200 parts of water, 20 parts of industrial monoammonium phosphate, 70 parts of diatomite and 40 parts of coconut oil acid polyglycerol ester.
Detailed description of preferred embodiments 3
A microbial agent containing biochemical fulvic acid potassium comprises the following raw materials in parts by weight: 800 parts of fulvic acid potassium (concentrated molasses liquid), 50 parts of microbial agent spore liquid (the effective viable count is more than or equal to 100 hundred million/g), 100 parts of water, 15 parts of industrial monoammonium phosphate, 45 parts of diatomite and 25 parts of coconut oil acid polyglycerol ester.
Detailed description of preferred embodiments 4
A microbial agent containing biochemical fulvic acid potassium comprises the following raw materials in parts by weight: 750 parts of fulvic acid potassium (concentrated molasses liquid), 50 parts of microbial agent spore liquid (the effective viable count is more than or equal to 100 hundred million/g), 80 parts of water, 12 parts of industrial monoammonium phosphate, 13 parts of diatomite and 18 parts of coconut oil acid polyglycerol ester.
Best mode for carrying out the invention
A microbial agent containing biochemical fulvic acid potassium comprises the following raw materials in parts by weight: 850 parts of fulvic acid potassium (concentrated molasses liquid), 50 parts of microbial agent spore liquid (the effective viable count is more than or equal to 100 hundred million/g), 12 parts of water, 18 parts of industrial monoammonium phosphate, 56 parts of diatomite and 31 parts of coconut oil acid polyglycerol ester.
Comparative experiment 1
The influence of the application of the microbial agent containing the biochemical fulvic acid potassium on the production of the ladybug in the embodiments 1 to 5 of the invention
1. Purpose of the experiment
The influence of the microbial agent containing the biochemical fulvic acid potassium of the spraying examples 1-5 as top dressing on the growth condition and yield of greenhouse vegetable ladybirds is investigated through field experiments in Jinningcounty of Kunming city, and the application effect is researched.
2. Test materials and methods
2.1 test materials
The selected crop in the experiment is the greenhouse vegetable gourd ladle, and the land is required to be flat, convenient to drain and irrigate and convenient to transport.
2.2 design of the experiment
The experiment designs 6 treatments, each treated base fertilizer is normally applied to a local conventional base fertilizer, the top dressing is the microbial agent containing the biochemical potassium fulvic acid in the examples 1-5 and the local conventional top dressing respectively, and the area of each cell is 30m2The cells are arranged randomly, protection rows are arranged, the planting density is calculated according to 3 thousands of plants per mu, and the arrangement is repeated for 3 times of treatment.
Treatment 1-5: and (3) local conventional fertilization is carried out, 100 g/mu of microbial inoculum containing the biochemical fulvic acid potassium in the examples 1-5 is diluted by 400 times with water for watering after 15d after sowing, and 200 g/mu of microbial inoculum containing the biochemical fulvic acid potassium in the examples 1-5 is diluted by 500 times with water for foliar spraying after 24 d.
Treatment 6 (control): and (3) applying fertilizer (a cloud leaf biological foliar fertilizer) conventionally, applying the cloud leaf biological foliar fertilizer 15 days after sowing, diluting with water 100 g/mu for 400 times of watering application, applying the cloud leaf biological foliar fertilizer 24 days later, diluting with water 200 g/mu for 500 times of foliar spraying.
3 results and analysis of the experiments
3.1 Effect of different treatments on agronomic traits of crops
According to the requirements of fertilizer test regulations, the whole test is subjected to normal field management in the aspects of cultivation, irrigation and drainage, management, fertilization, variety, density, pest control and the like.
As can be seen from Table 1, the application of the microbial agent containing biochemical fulvic acid has the effect of promoting the growth of the ladybug.
After 1-5 treatments, the plants are transplanted for 20 days and 30 days, and the height and the crown width of the plants are high. Wherein, the application of the microbial agent containing the biochemical potassium fulvate in the embodiment 3 after the treatment of the treatment 3 has the best agronomic characters, the height and the crown width of 20 plants after the transplantation are respectively 14.31cm and 48.67cm, and the height and the crown width of 35d plants after the transplantation are respectively 24.16cm and 54.28cm, which are obviously higher than those of a control group.
TABLE 1 influence of different treatments on the agronomic traits of ladybirds
3.2 Effect of different treatments on crop yield
As can be seen from Table 2, the treatment yields of treatments 1-3, i.e., the treatment with the microbial agent containing potassium fulvic acid were all higher than those of the control group, and the treatment yield of treatment 1, i.e., the treatment with the microbial agent containing potassium fulvic acid in example 3 was the highest, i.e., 203.58kg/30m2。
TABLE 2 Effect of different treatments on crop yield kg/30m2
4 conclusion of the test
From the above results, it can be seen that the microbial agent containing biochemical fulvic acid potassium in embodiments 1-5, especially the microbial agent in embodiment 3, of the present invention has the effects of promoting growth, increasing yield and improving economic benefits for ladybug, is convenient for transportation, is simple and convenient to apply, and is suitable for large-area popularization and use.
It should be understood that the above-described embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. Not all embodiments are exhaustive. All obvious changes and modifications which are obvious to the technical scheme of the invention are covered by the protection scope of the invention.
Claims (8)
1. A microbial agent containing biochemical fulvic acid potassium is characterized in that: the composite material comprises the following raw materials in parts by weight: 650-950 parts of fulvic acid potassium, 50 parts of microbial agent spore liquid, 10-200 parts of water, 10-20 parts of industrial monoammonium phosphate, 10-70 parts of diatomite and 10-40 parts of coconut oil acid polyglycerol ester;
the bacterial strains in the microbial agent spore liquid are bacillus amyloliquefaciens, bacillus subtilis, bacillus licheniformis and bacillus mucilaginosus spore liquid which are compounded according to the mass ratio of 1:1:1:1,
the preservation number of the bacillus amyloliquefaciens is CGMCC No. 20147;
the preservation number of the bacillus subtilis is CGMCC No. 20145;
the preservation number of the bacillus licheniformis is CGMCC No. 20150;
the preservation number of the bacillus mucilaginosus is CGMCC No. 20152.
2. The microbial agent containing biochemical potassium fulvate according to claim 1, wherein: the composite material comprises the following raw materials in parts by weight: 650 parts of potassium fulvate, 50 parts of microbial agent spore liquid, 10 parts of water, 10 parts of industrial monoammonium phosphate, 10 parts of diatomite and 10 parts of coconut oil acid polyglycerol ester.
3. The microbial agent containing biochemical potassium fulvate according to claim 1, wherein: the composite material comprises the following raw materials in parts by weight: 950 parts of potassium fulvate, 50 parts of microbial agent spore liquid, 200 parts of water, 20 parts of industrial monoammonium phosphate, 70 parts of diatomite and 40 parts of coconut oil acid polyglycerol ester.
4. The microbial agent containing biochemical potassium fulvate according to claim 1, wherein: the composite material comprises the following raw materials in parts by weight: 800 parts of potassium fulvate, 50 parts of microbial agent spore liquid, 100 parts of water, 15 parts of industrial monoammonium phosphate, 45 parts of diatomite and 25 parts of coconut oil acid polyglycerol ester.
5. The microbial agent containing biochemical potassium fulvate according to claim 1, wherein: the composite material comprises the following raw materials in parts by weight: 750 parts of potassium fulvate, 50 parts of microbial agent spore liquid, 80 parts of water, 12 parts of industrial monoammonium phosphate, 13 parts of diatomite and 18 parts of coconut oil acid polyglycerol ester.
6. The microbial agent containing biochemical potassium fulvate according to claim 1, wherein: the composite material comprises the following raw materials in parts by weight: 850 parts of potassium fulvate, 50 parts of microbial agent spore liquid, 12 parts of water, 18 parts of industrial monoammonium phosphate, 56 parts of diatomite and 31 parts of coconut oil acid polyglycerol ester.
7. The microbial agent containing biochemical potassium fulvate according to claim 1, wherein: the number of effective viable bacteria in the spore liquid of the microbial inoculum is more than or equal to 100 hundred million/g.
8. The microbial agent containing biochemical potassium fulvate according to claim 1, wherein: the preparation method comprises the following steps:
firstly, preparing raw materials: weighing potassium fulvate, microbial agent spore liquid, water, industrial monoammonium phosphate, diatomite and coconut oil acid polyglycerol ester for later use;
secondly, mixing materials: according to the weight parts, 650-950 parts of liquid fulvic acid potassium, 10-200 parts of water, 10-20 parts of industrial monoammonium phosphate, 10-70 parts of diatomite and 10-40 parts of coconut oil acid polyglycerol ester are stirred and mixed uniformly, the industrial monoammonium phosphate is heated, dissolved and mixed in the material, 50 parts of microbial agent spore liquid is added into the material, the mixture is stirred uniformly again, and the mixture is sieved by a 80-mesh sieve for later use after being mixed uniformly;
and step three, putting the material mixed liquor obtained by sieving in the step two into a centrifugal spray dryer, carrying out spray drying, and collecting powder to obtain the microbial agent containing the biochemical fulvic acid potassium.
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Cited By (2)
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CN113207530A (en) * | 2021-05-06 | 2021-08-06 | 张坤 | Green planting method for hazelnuts |
CN113277909A (en) * | 2021-06-08 | 2021-08-20 | 深圳市绿色农业生物科技有限公司 | Compound microbial agent of potassium fulvate |
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