CN107879866A - Improve microbial manure of protected crop quality and preparation method thereof - Google Patents
Improve microbial manure of protected crop quality and preparation method thereof Download PDFInfo
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- CN107879866A CN107879866A CN201711447012.9A CN201711447012A CN107879866A CN 107879866 A CN107879866 A CN 107879866A CN 201711447012 A CN201711447012 A CN 201711447012A CN 107879866 A CN107879866 A CN 107879866A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
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- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
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- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The invention discloses a kind of microbial manure and preparation method thereof for improving protected crop quality, belong to the preparation field of microbial manure.The present invention discloses a kind of microbial manure for improving protected crop quality first, including：Humic acid, crop material powder, bone meal, sheep manure, medical stone, composite bacteria agent, amino acid and clay.Wherein, the composite bacteria agent is made up of bacillus pumilus, arthrobacterium, Brevibacillus laterosporus, lactobacillus acidophilus and saccharomyces cerevisiae.The present invention further discloses a kind of method for preparing the microbial manure, including：Each component is well mixed, dry packing, produced.The invention also discloses application of the microbial manure in protected crop quality is improved.Microbial manure of the present invention contains several functions microorganism, rich in the amino acid needed for plant growth, mineral matter and various trace elements, plant growth can be promoted, improve the external condition of crop, increase active principle content, effectively improve protected crop quality.
Description
Technical field
The present invention relates to a kind of microbial manure, more particularly to a kind of microbial manure for improving protected crop quality, sheet
Invention further relates to the preparation method of the microbial manure, belongs to the preparation field of microbial manure.
Background technology
Since mid-twentieth century, requirement more and more higher of the people for agricultural output, therewith, the use of chemical fertilizer also by
Year increase, Chinese fertilizer consumption from 12,690,000 tons of 1980, to chemical fertilizer in 2017 demand up to 66,100,000 tons.And grow
The use of chemical fertilizer also results in a series of soil degradation problem since phase so that the growth of crop is severely impacted.Except this
Outside, in order to ensure crop yield, indiscriminate use of pesticide phenomenon is also more universal.In contrast, while yield is required, people
Requirement to crop quality also more and more higher, but due to agricultural chemicals, the irrational utilization of chemical fertilizer, cause crop agriculture it is residual it is exceeded,
A series of phenomenons such as the beneficiating ingredients such as nitrate content increase, vitamin reduction so that crop quality significantly reduces, and has a strong impact on
The health of the mankind is arrived.
Microbial manure has the characteristics that improved soil, improves utilization rate of fertilizer, green and healthy, in recent years increasingly by
The concern of people.But microbial manure is numerous in variety in the market, but quality varies, especially in protected crop field
Still lack a kind of microbial manure that can be effectively improved crop quality.Therefore, work can be effectively improved by needing exploitation one kind badly
The microbial manure of article matter, provided safeguard for the healthy living of the mankind.
The content of the invention
First technical problem to be solved by this invention is to provide a kind of microbial manure for improving protected crop quality,
The microbial manure can improve protected crop growing environment, effectively improve protected crop quality；
Second technical problem to be solved by this invention is to provide a kind of preparation method of the microbial manure.
In order to solve the above technical problems, the technical solution used in the present invention is：
The present invention discloses a kind of microbial manure for improving protected crop quality, including following each component first：Humic
Acid, crop material powder, bone meal, sheep manure, medical stone, composite bacteria agent, amino acid and clay.
The parts by weight of each component are：Humic acid 20-25 parts, crop material powder 40-50 parts, bone meal 3-8 parts, sheep manure 15-25
Part, medical stone 1-4 parts, composite bacteria agent 1-2 parts, amino acid 2-4 parts, clay 5-8 parts.Preferably, the parts by weight of each component are：It is rotten
Grow 20 parts of acid, 45 parts of crop material powder, 5 parts of bone meal, 20 parts of sheep manure, 2 parts of medical stone, 1 part of composite bacteria agent, 2 parts of amino acid, clay
5 parts.
The present invention uses the humic acid of high-purity.Wherein, the preparation of the crop material powder includes：By crop material powder
It is broken, cross more than 80 mesh and sieve.The present invention crop material is not particularly limited, maize straw, broomcorn straw, rice straw or
The crop materials such as wheat stalk are applied to the present invention.
The preparation of the sheep manure, medical stone, clay includes：Sheep manure, medical stone, clay are crushed respectively, crossed more than 80 mesh
Sieve；Wherein, the sheep manure is completely decomposed sheep manure.
Humic acid, crop material powder, bone meal, sheep manure, medical stone, amino acid, clay used in the present invention can pass through
Regular channel is commercially available.
Composite bacteria agent of the present invention is by bacillus pumilus (Bacillus pumilus), arthrobacterium
(Arthrobacter sp.), Brevibacillus laterosporus (Brevibacillus lacterospore), lactobacillus acidophilus
(Lactobacillus acidophilus) and saccharomyces cerevisiae (Saccharomyces cerevisiae) form.
By weight, the composite bacteria agent is by bacillus pumilus (Bacillus pumilus) 10-25 parts, arthrobacterium
(Arthrobacter sp.) 10-25 parts, Brevibacillus laterosporus (Brevibacillus lacterospore) 20-35 parts,
Lactobacillus acidophilus (Lactobacillus acidophilus) 5-15 parts, saccharomyces cerevisiae (Saccharomyces
Cerevisiae) 5-15 parts form.
As the preferred technical solution of the present invention, by weight, the composite bacteria agent is by bacillus pumilus
15 parts of (Bacillus pumilus), 25 parts of arthrobacterium (Arthrobacter sp.), Brevibacillus laterosporus
35 parts of (Brevibacillus lacterospore), 12 parts of lactobacillus acidophilus (Lactobacillus acidophilus), make
13 parts of compositions of brewer yeast (Saccharomyces cerevisiae).
More there is a choosing, the viable count in composite bacteria agent of the present invention is：Effective viable bacteria of five kinds of bacterium in composite bacteria agent
Number is all higher than or equal to 2.0 × 107cfu/g.The preparation process of the composite bacteria agent is mainly：Culture, fermentation expand it is numerous, mixing,
Concentration, dry.Conventional bacterial preparation process is well known to those skilled in the art.
Bacillus pumilus (Bacillus pumilus) in composite bacteria agent of the present invention, arthrobacterium
(Arthrobacter sp.), Brevibacillus laterosporus (Brevibacillus lacterospore), lactobacillus acidophilus
(Lactobacillus acidophilus), saccharomyces cerevisiae (Saccharomyces cerevisiae), there is Soluble phosphorus, Potassium release
With fixed nitrogen function, and a variety of enzymes can be secreted, enhancing crop is to the resistances of some diseases, while the microbial inoculum in the present invention is also
The pernicious gases such as ammonia in environment, hydrogen sulfide can be reduced, improve containing for benefit materials such as amino acid, soluble sugar in fruit
The content of the materials such as residues of pesticides and nitrate in amount, effectively reduction crop, and then significantly improve protected crop quality.
The present invention draws best of breed according to the different growth characteristics of each strain by a variety of proportioning repetition tests so that
There is effect complementation, the effect of Synergistic between each strain, increase active principle can be played in protected crop cultivation and is contained
Amount, improve the external condition of crop, improve crop quality the effect of.
The present invention is it is demonstrated experimentally that by bacillus pumilus (Bacillus pumilus), arthrobacterium (Arthrobacter
Sp.), Brevibacillus laterosporus (Brevibacillus lacterospore), lactobacillus acidophilus (Lactobacillus
Acidophilus) and five kinds of bacterium of saccharomyces cerevisiae (Saccharomyces cerevisiae) composition composite bacteria agent, can be obvious
Improvement cucumber quality, dry matter content, soluble sugar and the protein of cucumber show significant difference, and reach
The pole level of signifiance；It improves the significant effect of cucumber quality and is better than being answered by what lactobacillus acidophilus, saccharomyces cerevisiae and arthrobacterium formed
Microbial inoculum, or the composite bacteria agent that lactobacillus acidophilus, saccharomyces cerevisiae, bacillus pumilus and Brevibacillus laterosporus form are closed, or
The composite bacteria agent of person bacillus pumilus, Brevibacillus laterosporus and arthrobacterium composition.Illustrate above-mentioned five kinds of strain reasonable compatibilities
The purpose that effect is complementary, acts synergistically can be reached.
The present invention is to the bacillus pumilus (Bacillus pumilus) in the composite bacteria agent, arthrobacterium
(Arthrobacter sp.), Brevibacillus laterosporus (Brevibacillus lacterospore), lactobacillus acidophilus
(Lactobacillus acidophilus), the specific bacterial strain of saccharomyces cerevisiae (Saccharomyces cerevisiae) do not have
It is specifically limited.As the preferred technical solution of the present invention, bacillus pumilus (Bacillus pumilus) strain number
For ACCC 01897；Arthrobacterium (Arthrobacter sp.) strain number is ACCC 02724；Brevibacillus laterosporus
(Brevibacillus lacterospore) strain number is CICC 22362；Lactobacillus acidophilus (Lactobacillus
Acidophilus) strain number is ACCC 00157；Saccharomyces cerevisiae (Saccharomyces cerevisiae) strain number is
CICC 1895。
Strain used in the present invention is purchased from culture presevation administrative center of the Chinese Academy of Agricultural Sciences, China General Microbiological strain
Preservation administrative center etc..
The present invention further discloses a kind of method for preparing the microbial manure, comprise the following steps：By each component
It is well mixed, dry, packaging, produce.
The invention also discloses application of the described microbial manure in protected crop quality is improved.It is of the present invention micro-
In protected crop plantation, the top dressing as the base manure before plantation or after plantation uses bio-feritlizer.
Protected crop of the present invention includes but is not limited to：Any one or more in cucumber, muskmelon or tomato.
Microbial manure of the present invention has following functions：(1) improve that protected crop is disease-resistant, the characteristic such as degeneration-resistant, protect
Demonstrate,prove the healthy growth of protected crop so that the external condition of crop is obviously improved.(2) agricultural chemicals effectively reduced in protected crop is residual
Stay so that crop mouthfeel is more.(3) rehabilitating soil microbiota, improve soil physico-chemical property, promote point of nutrient in soil
Solution and utilization, increase the active constituent content in soil.(4) improving amino acid, protein, vitamin, carbohydrate etc. in crop has
Content of material is imitated, nitrate is reduced, degrading pesticide residues, improves crop interior quality.Crop full color, big can be made simultaneously
It is small, shape is more uniform.Because microbial manure of the present invention has above-mentioned function, therefore it ensure that the health of protected crop
Growth, improve the product quality of protected crop.
Application effect experiment on Muskmelon Planting shows that microbial manure of the present invention significantly improves the strain of muskmelon
High thick with stem, melon footpath shows increased trend with melon length；Improve the total sugar content of muskmelon, Vc and soluble solid content
Increased up to the level of signifiance.Application effect experiment on tomato planting shows that microbial manure of the present invention makes kind
The single fruit weight of eggplant substantially increases, and effectively increases Vc contents and solid content in tamato fruit, and effectively reduce it is unfavorable
In the nitrate and total acid content of health, the quality of greenhouse tomato is improved.
The application method of microbial manure of the present invention：Base manure can be made and top dressing is applied.Protected crop makees base manure, top dressing
Application in two dressings, spreads fertilizer over the fields or row replacement.Base manure accounts for full dose 60% or so, spreads fertilizer over the fields and even sowing is harrowed after ploughing, and top dressing is according to full fertilising
40% or so of amount spreads fertilizer over the fields, you can.Because the species of protected crop is different and applies the difference of chemical fertilizer amount, institute of the present invention simultaneously
State microbial manure and make every mu of dosage 10-40kg during base manure, make every mu of dosage 6-20kg during top dressing, while can be according to soil regime
And the difference of crop varieties makes appropriate adjustment.
Technical solution of the present invention compared with prior art, has the advantages that：
Microbial manure of the present invention contains several functions microorganism, has effect complementation, collaboration between each strain
The effect of synergy, increase active principle content can be played in protected crop cultivation, reducing nitrate and residues of pesticides etc. has
Evil content of material, improve the external condition of crop, improve crop quality the effect of.Meanwhile the composite bacteria agent in the fertilizer can divide
The organic matter in soil is solved, indispensable element is provided for plant growth.In addition, the beneficial bacterium in composite bacteria agent can effectively suppress to have
The growth of evil bacterium, reduces plant pest, the improvement to the external condition of crop such as size, color, shape etc. is played actively
Effect.Therefore, microbial manure of the present invention can improve protected crop growing environment, effectively improve protected crop quality.
Embodiment
The invention will now be further described with reference to specific embodiments, advantages of the present invention and feature will be with description and
It is apparent.It should be understood that the embodiment is only exemplary, any restrictions are not formed to the scope of the present invention.This area
Technical staff should be understood that without departing from the spirit and scope of the invention can to the details of technical solution of the present invention and
Form is modified or replaced, but these modifications or substitutions each fall within protection scope of the present invention.
1st, material
The present invention use high-purity humic acid, used humic acid, crop material powder, bone meal, sheep manure, medical stone,
Amino acid, clay can be purchased from respectively：Shanxi Huozhou Xingda humic acid plant, Hebei Lingshou County Peng Xian mineral products processings factory, the Inner Mongol
Su Nite Lv He agricultural trades Co., Ltd, the long Yongfa mineral products Co., Ltd in Shijiazhuang, the limited public affairs of Henan "Lotus" gourmet powder share
Department, Yangquan Shanxi Xin Tong humic acids Co., Ltd.In addition to above company, other regular producers can be also purchased from.
Used strain is respectively purchased from culture presevation administrative center of the Chinese Academy of Agricultural Sciences, China General Microbiological in the present invention
Culture presevation administrative center and China Agricultural University, wherein bacillus pumilus (Bacillus pumilus) strain number is
ACCC 01897；Arthrobacterium (Arthrobacter sp.) strain number is ACCC 02724；Brevibacillus laterosporus
(Brevibacillus lacterospore) strain number is CICC 22362；Lactobacillus acidophilus (Lactobacillus
Acidophilus) strain number is ACCC 00157；Saccharomyces cerevisiae (Saccharomyces cerevisiae) strain number is
CICC 1895。
The preparation of the microbial manure of embodiment 1
Each raw material is weighed by following portions by weight：25 parts of humic acid, 45 parts of crop material powder, 5 parts of bone meal, 15 parts of sheep manure,
2 parts of medical stone, 1 part of composite bacteria agent (15 parts of bacillus pumilus (Bacillus pumilus), arthrobacterium (Arthrobacter
Sp.) 25 parts, 30 parts of Brevibacillus laterosporus (Brevibacillus lacterospore), lactobacillus acidophilus
15 parts of (Lactobacillus acidophilus), 15 parts of saccharomyces cerevisiae (Saccharomyces cerevisiae)), amino
Sour 2 parts, 5 parts of clay.Each raw material can be obtained by buying above, and such as strain can be had by Beijing is wealthy up to the exploitation of industrial biotechnology
Limit company, culture presevation administrative center of the Chinese Academy of Agricultural Sciences, China General Microbiological culture presevation administrative center etc. obtain, and other are former
Material is bought according to regular channel.
Wherein, the preparation of the sheep manure, medical stone, clay includes：Sheep manure, medical stone, clay are crushed respectively, cross 80 mesh
Sieve above；Wherein, the sheep manure is completely decomposed sheep manure.
After each raw material weighed is fully mixed to uniformly, this microbial manure is produced.
The preparation of the microbial manure of embodiment 2
Each raw material is weighed by following portions by weight：25 parts of humic acid, 40 parts of crop material powder, 5 parts of bone meal, 20 parts of sheep manure,
1 part of medical stone, 2 parts of composite bacteria agent (25 parts of bacillus pumilus (Bacillus pumilus), arthrobacterium (Arthrobacter
Sp.) 25 parts, 30 parts of Brevibacillus laterosporus (Brevibacillus lacterospore), lactobacillus acidophilus
5 parts of (Lactobacillus acidophilus), 15 parts of saccharomyces cerevisiae (Saccharomyces cerevisiae)), amino acid
2 parts, 5 parts of clay.Preparation method is the same as embodiment 1.
Wherein, the preparation of the sheep manure, medical stone, clay includes：Sheep manure, medical stone, clay are crushed respectively, cross 80 mesh
Sieve above；Wherein, the sheep manure is completely decomposed sheep manure.
After each raw material weighed is fully mixed to uniformly, this microbial manure is produced.
The preparation of the microbial manure of embodiment 3
Each raw material is weighed by following portions by weight：20 parts of humic acid, 42 parts of crop material powder, 3 parts of bone meal, 20 parts of sheep manure,
4 parts of medical stone, 1 part of composite bacteria agent (20 parts of bacillus pumilus (Bacillus pumilus), arthrobacterium (Arthrobacter
Sp.) 18 parts, 34 parts of Brevibacillus laterosporus (Brevibacillus lacterospore), lactobacillus acidophilus
13 parts of (Lactobacillus acidophilus), 15 parts of saccharomyces cerevisiae (Saccharomyces cerevisiae)), amino
Sour 4 parts, 6 parts of clay.Preparation method is the same as embodiment 1.
Wherein, the preparation of the sheep manure, medical stone, clay includes：Sheep manure, medical stone, clay are crushed respectively, cross 80 mesh
Sieve above；Wherein, the sheep manure is completely decomposed sheep manure.
After each raw material weighed is fully mixed to uniformly, this microbial manure is produced.
The preparation of the microbial manure of embodiment 4
Each raw material (kg) is weighed in following ratios：Humic acid 20, crop material powder 40, bone meal 3, sheep manure 15, medical stone 1,
Composite bacteria agent 1, amino acid 2, clay 5；
Wherein, the preparation of the composite bacteria agent, each bacterium (kg) is weighed in following ratios：Bacillus pumilus (Bacillus
Pumilus) 10, arthrobacterium (Arthrobacter sp.) 10, Brevibacillus laterosporus (Brevibacillus
Lacterospore) 20, lactobacillus acidophilus (Lactobacillus acidophilus) 5, saccharomyces cerevisiae (Saccharomyces
Cerevisiae) 5, it is well mixed.
The preparation of the crop material powder includes：Crop material (maize straw) is crushed, crosses more than 80 mesh and sieves；
The preparation of the sheep manure, medical stone, clay includes：Sheep manure, medical stone, clay are crushed respectively, crossed more than 80 mesh
Sieve；Wherein, the sheep manure is completely decomposed sheep manure.
After each raw material is fully mixed to uniformly, this microbial manure is produced.
The preparation of the microbial manure of embodiment 5
Each raw material (kg) is weighed in following ratios：Humic acid 25, crop material powder 50, bone meal 8, sheep manure 25, medical stone 4,
Composite bacteria agent 2, amino acid 4, clay 8.
Wherein, the preparation of the composite bacteria agent, each bacterium (kg) is weighed in following ratios：Bacillus pumilus (Bacillus
Pumilus) 25, arthrobacterium (Arthrobacter sp.) 25, Brevibacillus laterosporus (Brevibacillus
Lacterospore) 35, lactobacillus acidophilus (Lactobacillus acidophilus) 15, saccharomyces cerevisiae
(Saccharomyces cerevisiae) 15, it is well mixed.
The preparation of the crop material powder includes：Crop material (wheat stalk) is crushed, crosses more than 80 mesh and sieves；
The preparation of the sheep manure, medical stone, clay includes：Sheep manure, medical stone, clay are crushed respectively, crossed more than 80 mesh
Sieve；Wherein, the sheep manure is completely decomposed sheep manure.
After each raw material is fully mixed to uniformly, this microbial manure is produced.
The preparation of the microbial manure of embodiment 6
Each raw material (kg) is weighed in following ratios：Humic acid 20, crop material powder 45, bone meal 5, sheep manure 20, medical stone 2,
Composite bacteria agent 1, amino acid 2, clay 5.
Wherein, the preparation of the composite bacteria agent, each bacterium (kg) is weighed in following ratios：Bacillus pumilus (Bacillus
Pumilus) 15, arthrobacterium (Arthrobacter sp.) 25, Brevibacillus laterosporus (Brevibacillus
Lacterospore) 35, lactobacillus acidophilus (Lactobacillus acidophilus) 12, saccharomyces cerevisiae
(Saccharomyces cerevisiae) 13, it is well mixed.
The preparation of the crop material powder includes：Crop material (rice straw) is crushed, crosses more than 80 mesh and sieves；
The preparation of the sheep manure, medical stone, clay includes：Sheep manure, medical stone, clay are crushed respectively, crossed more than 80 mesh
Sieve；Wherein, the sheep manure is completely decomposed sheep manure.
After each raw material is fully mixed to uniformly, this microbial manure is produced.
Application effect of 1 microbial manure of the present invention of test example on Turpan, Xinjiang Muskmelon Planting tests one, experiment material
Material
1st, for trying fertilizer：Microbial manure prepared by the embodiment of the present invention 1.
2nd, trial crops：Muskmelon.
2nd, test site and time
Test site：Turpan, Xinjiang city facility plastic greenhouse base
Test period：In August, 2010-November.
3rd, test method
Preferable, 20 sides of organic fertilizer/mu for examination ground soil fertility.August is colonized on the 15th.Experiment sets 3 processing.Handle A：This
Invention microbial manure 12kg/ mus；Handle B：Microbial manure 24kg/ mus of the present invention；Control：Do not apply microbial manure.It is small
0.3 mu of area's area, base are applied (ridging on fertilizer).Test period is in August, 2010-November.
4th, result and analysis
In terms of the quality of crop not only shows interior quality, its external condition is also a standard for evaluating its quality.
As can be seen from Table 1：Microbial manure of the present invention significantly improve muskmelon plant height and stem it is thick, melon footpath and melon, which grow performance, to be increased
Trend.
The plant growth condition of table 1
Quality determination result shows (table 2) that microbial manure of the present invention improves total sugar content to muskmelon, handles B Vc
Content adds 2.1mg/100g than CK, has reached the level of signifiance；Processing A, B significantly increase soluble solid.
The muskmelon attributional analysis of table 2
2 microbial manure of the present invention of test example is on the tomato planting of Daxing District institute of agricultural sciences transformation of scientific and technical result base
Application effect is tested
First, test material
1st, for trying fertilizer：Microbial manure prepared by the embodiment of the present invention 6.
2nd, trial crops：Tomato.
2nd, test site and time
Test site：Daxing District institute of agricultural sciences transformation of scientific and technical result base.
Test period：In July, 2009-November.
3rd, test method
Experiment sets two processing altogether, and the plot area each handled is 62.8m2：Processing 1 is microbial manure of the present invention
(12kg/ mus, as base manure)+conventional fertilizer application；Processing CK is conventional fertilizer application.As a result tomato single fruit weight, Vc contents, solid are determined afterwards
Thing, nitrate and total acid content.
4th, result and analysis
From table 3 it is observed that using compared with conventional fertilizer application, tomato single fruit weight, Vc contain after microbial manure of the present invention
Amount, solid content have increase, and be unfavorable for health nitrate and total acid content decrease.
The Tomato Quality index determining of table 3
It can be drawn from above index：Vitamin C in tamato fruit can be effectively improved using microbial manure of the present invention
And soluble solid content, nitrate content is effectively reduced, improves the quality of greenhouse tomato.
The strain Optimum Experiment of composite bacteria agent in 3 microbial manure of the present invention of test example
First, test material and place
Test and carried out in 2 months 2016 in Henan Province Fugou County, soil fertility is uniform, and trial crops are cucumber, cell face
Product is 3 × 10m2, each cell plantation cucumber 2 ridge, line-spacing 60cm, spacing in the rows 30cm, harvesting in June.
2nd, experimental design
Experiment is divided into control and processing, and processing is respectively 300 times of 5 groups of composite bacteria agent 10L of dilution, compare for equivalent it is clear
Water, method of application are to spray.
The constituent and proportioning of 5 groups of composite bacteria agents be respectively,
Microbial inoculum 1：15 parts of bacillus megaterium (Bacillus megaterium), arthrobacterium (Arthrobacter sp.)
25 parts, 35 parts of Brevibacillus laterosporus (Brevibacillus lacterospore), lactobacillus acidophilus (Lactobacillus
Acidophilus) 12 parts, 13 parts of saccharomyces cerevisiae (Saccharomyces cerevisiae)；
Microbial inoculum 2：15 parts of bacillus pumilus (Bacillus pumilus), arthrobacterium (Arthrobacter sp.) 25
Part, 35 parts of bacillus subtilis (Bacillus subtilis), lactobacillus acidophilus (Lactobacillus acidophilus)
12 parts, 13 parts of saccharomyces cerevisiae (Saccharomyces cerevisiae)；
Microbial inoculum 3：15 parts of bacillus pumilus (Bacillus pumilus), arthrobacterium (Arthrobacter sp.) 25
Part, 35 parts of Brevibacillus laterosporus (Brevibacillus lacterospore), Lactobacillus plantarum (Lactobacillus
Plantarum) 12 parts, 13 parts of saccharomyces cerevisiae (Saccharomyces cerevisiae)；
Microbial inoculum 4：15 parts of bacillus pumilus (Bacillus pumilus), 25 parts of brevibacterium (Brevibacterium),
35 parts of Brevibacillus laterosporus (Brevibacillus lacterospore), lactobacillus acidophilus (Lactobacillus
Acidophilus) 12 parts, 13 parts of saccharomyces cerevisiae (Saccharomyces cerevisiae)；
Microbial inoculum 5：15 parts of bacillus pumilus (Bacillus pumilus), arthrobacterium (Arthrobacter sp.) 25
Part, 35 parts of Brevibacillus laterosporus (Brevibacillus lacterospore), lactobacillus acidophilus (Lactobacillus
Acidophilus) 12 parts, 13 parts of saccharomyces cerevisiae (Saccharomyces cerevisiae)；
3rd, test index
After cucumber maturation, measure dry matter content, soluble sugar, protein content.
4th, result and analysis
By being detected to the cucumber quality for applying various combination microbial inoculum, as a result as shown in table 4：Compared with the control, bacterium
Dry matter content, soluble sugar and the protein of agent 5 show significant difference, and have reached the pole level of signifiance；Other
Although microbial inoculum processing also has the effect for being significantly higher than control, the best results of microbial inoculum 5.
It can be obtained by analysis：By 15 parts of bacillus pumilus (Bacillus pumilus), arthrobacterium (Arthrobacter
Sp.) 25 parts, 35 parts of Brevibacillus laterosporus (Brevibacillus lacterospore), lactobacillus acidophilus
13 parts of compositions of 12 parts of (Lactobacillus acidophilus), saccharomyces cerevisiae (Saccharomyces cerevisiae)
Composite bacteria agent, improvement cucumber quality that can be more obvious, illustrate that above-mentioned strain reasonable compatibility can reach that effect is complementary, collaboration
The purpose of effect.
The cucumber quality of table 4 compares
The present invention is further by testing bacillus pumilus (Bacillus pumilus) 10-25 parts, arthrobacterium
(Arthrobacter sp.) 10-25 parts, Brevibacillus laterosporus (Brevibacillus lacterospore) 20-35 parts,
Lactobacillus acidophilus (Lactobacillus acidophilus) 5-15 parts, saccharomyces cerevisiae (Saccharomyces
Cerevisiae) 5-15 parts (parts by weight), the effect for improving protected crop quality are more notable.
The various functions of the in summary bacterial strain of strain used in the present invention, and finally play the work(for improving protected crop quality
Energy.
Claims (10)
1. a kind of microbial manure for improving protected crop quality, it is characterised in that including following each component：Humic acid, crop
Powder of straw, bone meal, sheep manure, medical stone, composite bacteria agent, amino acid and clay.
2. according to the microbial manure described in claim 1, it is characterised in that the parts by weight of each component are：Humic acid 20-25
Part, crop material powder 40-50 parts, bone meal 3-8 parts, sheep manure 15-25 parts, medical stone 1-4 parts, composite bacteria agent 1-2 parts, amino acid 2-
4 parts, clay 5-8 parts.
3. according to the microbial manure described in claim 1, it is characterised in that the parts by weight of each component are：20 parts of humic acid, make
45 parts of thing powder of straw, 5 parts of bone meal, 20 parts of sheep manure, 2 parts of medical stone, 1 part of composite bacteria agent, 2 parts of amino acid, 5 parts of clay.
4. according to the microbial manure described in claims 1 to 3 any one, it is characterised in that：The system of the crop material powder
It is standby to include：Crop material is crushed, crosses more than 80 mesh and sieves；
The preparation of the sheep manure, medical stone, clay includes：Sheep manure, medical stone, clay are crushed respectively, crosses more than 80 mesh and sieves；Its
In, the sheep manure is completely decomposed sheep manure.
5. according to the microbial manure described in claims 1 to 3 any one, it is characterised in that the composite bacteria agent is by short and small
Bacillus (Bacillus pumilus), arthrobacterium (Arthrobacter sp.), Brevibacillus laterosporus
(Brevibacillus lacterospore), lactobacillus acidophilus (Lactobacillus acidophilus) and saccharomyces cerevisiae
(Saccharomyces cerevisiae) is formed.
6. according to the microbial manure described in claim 5, it is characterised in that：By weight, the composite bacteria agent is by short and small
Bacillus (Bacillus pumilus) 10-25 parts, arthrobacterium (Arthrobacter sp.) 10-25 parts, the short gemma of side spore
Bacillus (Brevibacillus lacterospore) 20-35 parts, lactobacillus acidophilus (Lactobacillus acidophilus)
5-15 parts, saccharomyces cerevisiae (Saccharomyces cerevisiae) 5-15 parts composition；
Preferably, by weight, the composite bacteria agent is by 15 parts of bacillus pumilus (Bacillus pumilus), arthrobacterium
25 parts of (Arthrobacter sp.), 35 parts of Brevibacillus laterosporus (Brevibacillus lacterospore), acidophilus breast
13 parts of 12 parts of bacillus (Lactobacillus acidophilus), saccharomyces cerevisiae (Saccharomyces cerevisiae) groups
Into.
A kind of 7. method for preparing microbial manure described in claim 1 to 6 any one, it is characterised in that including following step
Suddenly：Each component is well mixed, dries, packaging, produces.
8. application of the microbial manure in protected crop quality is improved described in claim 1 to 6 any one.
9. according to the application described in claim 8, it is characterised in that：The microbial manure is used as planting in protected crop plantation
Top dressing after base manure or plantation before plant uses.
10. according to the application described in claim 8, it is characterised in that the protected crop includes：In cucumber, muskmelon or tomato
Any one or more.
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| CN111072420A (en) * | 2019-12-28 | 2020-04-28 | 武汉美瑞农业科技有限公司 | Compound amino acid microbial agent and preparation method thereof |
| CN111826319A (en) * | 2020-07-31 | 2020-10-27 | 西南林业大学 | Microbial growth promoter and application thereof |
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| CN113387757A (en) * | 2021-07-06 | 2021-09-14 | 石河子大学 | Composite microbial fertilizer and preparation method and application thereof |
| CN117305188A (en) * | 2023-11-27 | 2023-12-29 | 山东中微众康生物科技有限公司 | Lactobacillus acidophilus RZKLa0701 capable of promoting bone growth of children and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110563517A (en) * | 2018-06-06 | 2019-12-13 | 王家芬 | organic bacterial fertilizer containing sheep manure, medical stone and animal bone meal for agricultural production and production method thereof |
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| CN113387757A (en) * | 2021-07-06 | 2021-09-14 | 石河子大学 | Composite microbial fertilizer and preparation method and application thereof |
| CN117305188A (en) * | 2023-11-27 | 2023-12-29 | 山东中微众康生物科技有限公司 | Lactobacillus acidophilus RZKLa0701 capable of promoting bone growth of children and application thereof |
| CN117305188B (en) * | 2023-11-27 | 2024-02-13 | 山东中微众康生物科技有限公司 | Lactobacillus acidophilus RZKLa0701 capable of promoting bone growth of children and application thereof |
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