CN104591929A - Preparation process or bio-organic fertilizer - Google Patents
Preparation process or bio-organic fertilizer Download PDFInfo
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- CN104591929A CN104591929A CN201510057765.3A CN201510057765A CN104591929A CN 104591929 A CN104591929 A CN 104591929A CN 201510057765 A CN201510057765 A CN 201510057765A CN 104591929 A CN104591929 A CN 104591929A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D1/00—Fertilisers containing potassium
- C05D1/02—Manufacture from potassium chloride or sulfate or double or mixed salts thereof
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Soil Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Fertilizers (AREA)
Abstract
The invention belongs to the technical field of fertilizer, and discloses a preparation process of a bio-organic fertilizer. The preparation process comprises the following steps: uniformly mixing and stirring a microbial preparation and an ingredient A to obtain a mixture I, culturing the mixture I at the temperature of 30 DEG C for 12 hours, then charging an ingredient B and an ingredient C, stirring for 3 minutes at a speed of 100 turns per minute, and finally carrying out the low-temperature drying and granulation to obtain the bio-organic fertilizer. The preparation process is simple and easy, short in process flow and low in cost.
Description
Technical field
The invention belongs to technical field of fertilizers, be specifically related to a kind of preparation technology of biological organic fertilizer.
Background technology
Biological organic fertilizer refers to microorganisms with specific functions and is mainly originate and have the fertilizer of microbial fertilizer and fertilizer effect through the class that harmless treatment, the organic materials that becomes thoroughly decomposed are composited concurrently with plant and animal residues (as feces of livestock and poultry, agricultural crop straw etc.).Biological organic fertilizer is compared with chemical fertilizer: 1) biological organic fertilizer nutritive element is complete; Chemical fertilizer nutritive element only has one or more.2) biological organic fertilizer can improve soil; Chemical fertilizer often use can cause soil compaction.3) biological organic fertilizer can improve product quality; Chemical fertilizer application too much causes product quality inferior.4) biological organic fertilizer can improve rhizosphere microorganisms of crops group, improves the resistant to diseases and insects of plant; Chemical fertilizer is then that crop microbial population is single, easily disease and pest occurs.
At present, in order to improve grain yield, come into operation in a large number and but to have ignored the disadvantageous effect of chemical fertilizer to the quality of agricultural-food, soil compaction, underground water and ecotope while inorganic fertilizer carries out carrying product in a lot of farmland, organic matter in soil is sharply declined, destroy original soil ecology balance, soil compaction, water and soil pollution, environmental degradation, disruption of ecological balance, agriculture production cost increases and agricultural product quality decline contradiction, thus has occurred that Higher output is not accompanied by a higher income and the vicious cycle of environmental pollution madness such as (grow) baths of rivers and lakes water.
Agricultural chemicals is the effective means of control diseases and pests of agronomic crop and aquaculture disease, the use of a large amount of agricultural chemicals, becomes main " killer " of food safety and harm health of people.Chemical pesticide is different by its function, is mainly divided into Insecticides (tech) & Herbicides (tech) and sterilant three major types.Different according to chemical structure, the broad varietys such as organochlorine class, organic phosphates, pyrethroid lipid, carboxylamine lipid and inorganic pesticide can be divided into.Organophosphorus pesticide has become one of maximum agricultural chemicals of usage quantity, and the kind of the organophosphorus pesticide that China produces just has kind more than 20, and annual production, more than 100kt, accounts for more than 80% of China's agricultural chemicals ultimate production.According to statistics, China's applying pesticides area is at 20,000,000,000 hm
2above, every annual consumption more than 500,000 tons.These agricultural chemicals used, have and over halfly residue in soil, bring very large potential safety hazard to grain security.
There are the production and sales of some biological organic fertilizers at present, but it is lasting not mostly to there is fertilizer efficiency, and cost is higher, and does not possess the function of rehabilitating soil remains of pesticide, therefore, the preparation technology researching and developing a kind of novel biological fertilizer is the technical barrier that we need to solve.
Summary of the invention
The present invention be directed to the defect of prior art biological organic fertilizer production technology, the preparation technology of a kind of biological organic fertilizer proposed, this technique simple possible, technical process is short, with low cost, biological organic fertilizer prepared by this technique not only possesses good fertilizer efficiency, but also can remove the pesticide residue in soil, has repaired contaminated soil.
For achieving the above object, the present invention is by the following technical solutions:
A preparation technology for biological organic fertilizer, it comprises the steps: that, by microbial preparation and batching A mixing and stirring, cultivate 12 hours for 30 DEG C, then interpolation batching B and batching C, 100 turns/min stir 3min, finally carry out cryodrying, granulation and get final product; Described prilling temperature is lower than 40 DEG C, and the water content of described biological organic fertilizer is 6-8%(w/w).
Described biological organic fertilizer is prepared from by the raw material of following weight part: batching A 10-12 part, batching B 4-6 part, batching C 3-5 part, and microbial preparation 1-2 part;
Described batching A is prepared as follows and forms: by wheat bran and dregs of beans by the weight ratio mixing and stirring of 1:1, add the hydrochloric acid soln of 0.1mol/L subsequently, be not as the criterion, soak 12 hours, then 80 DEG C of oven dry, final powder is broken into powder; Pericarppium arachidis hypogaeae is dried, is ground into peanut hull meal with pulverizer; Above-mentioned powder, peanut hull meal and water are added in stirred pot according to the mass ratio of 1:1:1, after the speed stirring 15min of 500 revs/min, material in stirred pot is sent in reactor, water vapor is passed in reactor, maintain the temperature of reactor more than 95 DEG C, insulation reaction 30min, last naturally cooling and get final product; The particle diameter of above-mentioned powder and peanut hull meal all controls between 50-100 order;
Described batching B is prepared as follows and forms:
First calcite is carried out pulverization process and obtain calcite in powder, then mix with Repone K, humic acids and the peat composed of rotten mosses weight ratio according to 1:1:1:1, to obtain final product; The particle diameter of described calcite in powder, Repone K, humic acids and the peat composed of rotten mosses all controls between 50-100 order;
Described batching C is prepared as follows and forms:
First the particle diameter controlling sawdust and chitosan is 50-100 order, is then mixed by sawdust, chitosan and the glycerine mass ratio according to 1:1:1, to obtain final product.
Described microbial preparation is prepared as follows and forms:
Acinetobacter bauamnnii, Pseudomonas fluorescens, enterococcus faecalis, micrococcus luteus and azotobacter chroococcum being trained respectively concentration is 1 × 10
8the bacterium liquid of individual/ml, according to 3:3:2:1:1 volume ratio mixing and stirring and get final product.
Described Acinetobacter bauamnnii is Acinetobacter bauamnnii (Acinetobacter baumanii) ATCC 19606;
Described Pseudomonas fluorescens is Pseudomonas fluorescens (P.Fluorescens) ATCC 49642;
Described enterococcus faecalis is enterococcus faecalis (Enterococcus faecalis) ATCC 29212;
Described micrococcus luteus is micrococcus luteus (Micrococcus luteus) ATCC 49442;
Described azotobacter chroococcum is azotobacter chroococcum (Azotobacter chroococcum) ATCC 4412.
The using method of above-mentioned biological organic fertilizer can be: sow during cultivated soil, and the applied amount on every mu of ground is 15-20kg; Also alternate manner can be adopted to sow, and amount of application regulates and controls voluntarily, generally must not more than 50kg/ mu.
Bacterial classification of the present invention all can be bought from commercial channel such as American Type Culture collection warehousings (ATCC) and obtain.The enlarged culturing method of bacterial strain of the present invention can record according to document or the record of prior art is carried out, and is not innovative point of the present invention herein, as space is limited, does not repeat one by one.
The beneficial effect that the present invention obtains is main but be not limited to the following aspects:
Biological organic fertilizer technique simple possible of the present invention, flow process is short, with low cost; Biological organic fertilizer material combination prepared by present invention process is reasonable, by effectively utilizing agricultural waste material and natural mineral matter, in conjunction with microbial technique, makes fertilizer more lasting; Add in fertilizer of the present invention and supplement multiple nutriment, fertilizer efficiency is comprehensive, strengthens crop disease-resistant anti-adversity ability; Biological organic fertilizer of the present invention can repair pesticide contaminated soil effectively, and removal effect is good, ensure that grain security; Reasonable compatibility between each bacterial classification in microbial preparation of the present invention, synergy is strong, and active high breeding is fast.
Embodiment
Technical scheme in the application is understood better in order to make those skilled in the art person, below in conjunction with the application's specific embodiment, the technical scheme of the application is clearly and completely described, obviously, described embodiment is only some embodiments of the present application, instead of whole embodiments.Based on the embodiment in the application, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, should belong to the scope of protection of the invention.
Embodiment 1
A preparation technology for biological organic fertilizer, it comprises the steps: that, by microbial preparation and batching A mixing and stirring, cultivate 12 hours for 30 DEG C, then interpolation batching B and batching C, 100 turns/min stir 3min, finally carry out cryodrying, granulation and get final product; Described prilling temperature 30 DEG C, the water content of described biological organic fertilizer is 6%.
Described biological organic fertilizer is prepared from by the raw material of following weight part: batching A 10 parts, batching B 4 parts, batching C 3 parts, and microbial preparation 1 part;
Described batching A is prepared as follows and forms: by wheat bran and dregs of beans by the weight ratio mixing and stirring of 1:1, add the hydrochloric acid soln of 0.1mol/L subsequently, be not as the criterion, soak 12 hours, then 80 DEG C of oven dry, final powder is broken into powder; Pericarppium arachidis hypogaeae is dried, is ground into peanut hull meal with pulverizer; Above-mentioned powder, peanut hull meal and water are added in stirred pot according to the mass ratio of 1:1:1, after the speed stirring 15min of 500 revs/min, material in stirred pot is sent in reactor, water vapor is passed in reactor, maintain the temperature of reactor at 95 DEG C, insulation reaction 30min, last naturally cooling and get final product; The particle diameter of above-mentioned powder and peanut hull meal all controls at 50 orders;
Described batching B is prepared as follows and forms:
First calcite is carried out pulverization process and obtain calcite in powder, then mix with Repone K, humic acids and the peat composed of rotten mosses weight ratio according to 1:1:1:1, to obtain final product; The particle diameter of described calcite in powder, Repone K, humic acids and the peat composed of rotten mosses all controls at 50 orders;
Described batching C is prepared as follows and forms:
First the particle diameter controlling sawdust and chitosan is 50 orders, is then mixed by sawdust, chitosan and the glycerine mass ratio according to 1:1:1, to obtain final product.
Described microbial preparation is prepared as follows and forms:
Acinetobacter bauamnnii, Pseudomonas fluorescens, enterococcus faecalis, micrococcus luteus and azotobacter chroococcum being trained respectively concentration is 1 × 10
8the bacterium liquid of individual/ml, according to 3:3:2:1:1 volume ratio mixing and stirring and get final product.
The using method of above-mentioned biological organic fertilizer is: sow during cultivated soil, and the applied amount on every mu of ground is 20kg.
Embodiment 2
A preparation technology for biological organic fertilizer, it comprises the steps: that, by microbial preparation and batching A mixing and stirring, cultivate 12 hours for 30 DEG C, then interpolation batching B and batching C, 100 turns/min stir 3min, finally carry out cryodrying, granulation and get final product; Described prilling temperature is 31 DEG C, and the water content of described biological organic fertilizer is 8%.
Described biological organic fertilizer is prepared from by the raw material of following weight part: batching A 12 parts, batching B 6 parts, batching C 5 parts, and microbial preparation 2 parts;
Described batching A is prepared as follows and forms: by wheat bran and dregs of beans by the weight ratio mixing and stirring of 1:1, add the hydrochloric acid soln of 0.1mol/L subsequently, be not as the criterion, soak 12 hours, then 80 DEG C of oven dry, final powder is broken into powder; Pericarppium arachidis hypogaeae is dried, is ground into peanut hull meal with pulverizer; Above-mentioned powder, peanut hull meal and water are added in stirred pot according to the mass ratio of 1:1:1, after the speed stirring 15min of 500 revs/min, material in stirred pot is sent in reactor, water vapor is passed in reactor, maintain the temperature of reactor at 99 DEG C, insulation reaction 30min, last naturally cooling and get final product; The particle diameter of above-mentioned powder and peanut hull meal all controls at 100 orders;
Described batching B is prepared as follows and forms:
First calcite is carried out pulverization process and obtain calcite in powder, then mix with Repone K, humic acids and the peat composed of rotten mosses weight ratio according to 1:1:1:1, to obtain final product; The particle diameter of described calcite in powder, Repone K, humic acids and the peat composed of rotten mosses all controls at 100 orders;
Described batching C is prepared as follows and forms:
First the particle diameter controlling sawdust and chitosan is 100 orders, is then mixed by sawdust, chitosan and the glycerine mass ratio according to 1:1:1, to obtain final product.
Described microbial preparation is prepared as follows and forms:
Acinetobacter bauamnnii, Pseudomonas fluorescens, enterococcus faecalis, micrococcus luteus and azotobacter chroococcum being trained respectively concentration is 1 × 10
8the bacterium liquid of individual/ml, according to 3:3:2:1:1 volume ratio mixing and stirring and get final product.
Described Acinetobacter bauamnnii is Acinetobacter bauamnnii (Acinetobacter baumanii) ATCC 19606(Infect Immun. 2012 Mar; 80 (3): 1015-24);
Described Pseudomonas fluorescens is Pseudomonas fluorescens (P.Fluorescens) ATCC49642(WO2003066873A);
Described enterococcus faecalis is enterococcus faecalis (Enterococcus faecalis) ATCC 29212(In Vivo. 2009 Jan-Feb; 23 (1): 81-7);
Described micrococcus luteus is micrococcus luteus (Micrococcus luteus) ATCC 49442(APPLIED AND ENVIRONMENTAL MICROBIOLOGY1992, p.3423-3425);
Described azotobacter chroococcum is azotobacter chroococcum (Azotobacter chroococcum) ATCC4412(Production of exocellular polysaccharide by Azotobacter chroococcum. Appl Biochem Biotechnol. 1991 Sep; 30 (3): 273-84.).
The using method of above-mentioned biological organic fertilizer can be: sow during cultivated soil, and the applied amount on every mu of ground is 15kg.
Embodiment 3
The manure trial of biological organic fertilizer prepared by present invention process
One, the effect of plantation Chinese sorghum is fat tests:
Control group and experimental group all adopt composite fertilizer (nitrogen-phosphorus-potassium=23-7-6), and the soil property in experimental plot is identical with planting conditions, and area is 10 mu; Rate of fertilizer application is 50kg/ mu; Additionally sow biological organic fertilizer prepared by the embodiment of the present invention 2 technique when experimental group employing is ploughed, amount of application is 15kg/ mu; Concrete test-results is in table 1:
Table 1
Note: composite fertilizer calculates according to 2.7 yuan/kg, fertilizer prepared by the present invention calculates according to 2.1 yuan/kg, and Chinese sorghum is according to 2.8 yuan/kg.
Two, the experiment of Ipomoea batatas fertilizer efficiency is planted:
Arrange two process experimental plots, area is 10 mu, is control group and experimental group.Control group and experimental group all adopt composite fertilizer (N16-P22-K7), and the soil property in experimental plot is identical with planting conditions, and rate of fertilizer application is 60kg/ mu; Additionally sow biological organic fertilizer prepared by the embodiment of the present invention 2 technique when experimental group employing is ploughed, amount of application is 15kg/ mu; Experimental group per mu yield is 2768Kg, and control group per mu yield is 2432Kg, per muly increases extra earning more than hundred yuan.
Embodiment 4
The repairing test of pesticide contaminated soil
Fetch earth from Crop experiment field, Hedong District as examination soil sample, be divided into control group and experimental group, controlling at the content of 2,4-d butyl esters of control group and experimental group is 10mg/ per kilogram soil, controls the water content more than 30% of soil; Experimental group sows biological organic fertilizer prepared by the embodiment of the present invention 2 technique, and control group does not adopt any process.Within 5 days and 10 days, sample respectively, according to the sample preparation of Accelerate solvent extraction (ASE) method, in hexanaphthene extraction soil sample 2,4-d butyl ester, the high performance liquid phase detection method of 2,4-d butyl esters residual in soil measures 2,4-d butyl ester chromatographic condition: chromatographic column Hypersil C18 post; Moving phase: V (methyl alcohol): V (acetonitrile): V (citric acid solution)=29:21:50; Flow velocity: 1.5mL/min; Determined wavelength 230nm, column temperature: room temperature; Sample size: 15 μ 1.Degradation rate (%)=(1-experimental group residual quantity/control group residual quantity) × 100%.After 5 days, the degradation rate of 2,4-d butyl ester is 93.83%, 10 days degradation rates is 98.96%.
Although above done detailed explanation with general explanation and embodiment to this case, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, amendment done without departing from theon the basis of the spirit of the present invention or improvement, all belong to the scope of protection of present invention.
Claims (4)
1. a preparation technology for biological organic fertilizer, is characterized in that, described technique comprises the steps: microbial preparation and batching A mixing and stirring, cultivate 12 hours for 30 DEG C, then add batching B and stir 3min with batching C, 100 turns/min, finally carry out cryodrying, granulation and get final product; The weight ratio of described batching A, batching B, prepare burden C and microbial preparation is 10-12:4-6:3-5:1-2.
2. technique according to claim 1, it is characterized in that, described batching A is prepared as follows and forms: by wheat bran and the dregs of beans weight ratio mixing and stirring by 1:1, add the hydrochloric acid soln of 0.1mol/L subsequently, be not as the criterion, soak 12 hours, then 80 DEG C of oven dry, final powder is broken into powder; Pericarppium arachidis hypogaeae is dried, is ground into peanut hull meal with pulverizer; Above-mentioned powder, peanut hull meal and water are added in stirred pot according to the mass ratio of 1:1:1, after the speed stirring 15min of 500 revs/min, material in stirred pot is sent in reactor, water vapor is passed in reactor, maintain the temperature of reactor more than 95 DEG C, insulation reaction 30min, last naturally cooling and get final product; The particle diameter of above-mentioned powder and peanut hull meal all controls between 50-100 order;
Described batching B is prepared as follows and forms:
First calcite is carried out pulverization process and obtain calcite in powder, then mix with Repone K, humic acids and the peat composed of rotten mosses weight ratio according to 1:1:1:1, to obtain final product; The particle diameter of described calcite in powder, Repone K, humic acids and the peat composed of rotten mosses all controls between 50-100 order;
Described batching C is prepared as follows and forms:
First the particle diameter controlling sawdust and chitosan is 50-100 order, is then mixed by sawdust, chitosan and the glycerine mass ratio according to 1:1:1, to obtain final product;
Described microbial preparation is prepared as follows and forms:
Acinetobacter bauamnnii, Pseudomonas fluorescens, enterococcus faecalis, micrococcus luteus and azotobacter chroococcum being trained respectively concentration is 1 × 10
8the bacterium liquid of individual/ml, according to 3:3:2:1:1 volume ratio mixing and stirring and get final product.
3. technique according to claim 2, is characterized in that,
Described Acinetobacter bauamnnii is Acinetobacter bauamnnii (Acinetobacter baumanii) ATCC 19606;
Described Pseudomonas fluorescens is Pseudomonas fluorescens (P.Fluorescens) ATCC 49642;
Described enterococcus faecalis is enterococcus faecalis (Enterococcus faecalis) ATCC 29212;
Described micrococcus luteus is micrococcus luteus (Micrococcus luteus) ATCC 49442;
Described azotobacter chroococcum is azotobacter chroococcum (Azotobacter chroococcum) ATCC 4412.
4. let alone the technique described in one according to claim 1-3, it is characterized in that, described prilling temperature is lower than 40 DEG C, and the water content of described biological organic fertilizer is 6-8%.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106045701A (en) * | 2016-06-07 | 2016-10-26 | 内蒙古阜丰生物科技有限公司 | Compound fertilizer prepared from waste through fermentation with threonine |
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| CN102173925A (en) * | 2011-01-10 | 2011-09-07 | 艾应伟 | Multi-element composite microbial fertilizer |
| KR20130092275A (en) * | 2012-02-10 | 2013-08-20 | 주식회사 한우리비료 | Method for manufacturing sulfer fertilizer and sulfer fertilizer thereof |
| CN104003817A (en) * | 2014-06-24 | 2014-08-27 | 齐鲁工业大学 | Disease-resistant compound biofertilizer special for corn and preparation method thereof |
| CN104003804A (en) * | 2014-06-09 | 2014-08-27 | 山东红日阿康化工股份有限公司 | Moisture-retaining and nitrogen-fixing compound microbial fertilizer and preparation method thereof |
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- 2015-02-04 CN CN201510057765.3A patent/CN104591929A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03228888A (en) * | 1990-02-05 | 1991-10-09 | Isao Osuga | Production of organic fermented fertilizer |
| CN1546438A (en) * | 2003-12-12 | 2004-11-17 | 华东理工大学 | Multifunctional chitin release-controlled fertilizer |
| CN102001893A (en) * | 2011-01-07 | 2011-04-06 | 四川大学 | Preparation method of polyelement composite microbial fertilizer containing organic iron element |
| CN102173925A (en) * | 2011-01-10 | 2011-09-07 | 艾应伟 | Multi-element composite microbial fertilizer |
| KR20130092275A (en) * | 2012-02-10 | 2013-08-20 | 주식회사 한우리비료 | Method for manufacturing sulfer fertilizer and sulfer fertilizer thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106045701A (en) * | 2016-06-07 | 2016-10-26 | 内蒙古阜丰生物科技有限公司 | Compound fertilizer prepared from waste through fermentation with threonine |
| CN106045701B (en) * | 2016-06-07 | 2019-07-23 | 内蒙古阜丰生物科技有限公司 | The compound fertilizer prepared using Threonine Fermentation waste |
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