CN106906168A - A kind of spirit stillage decomposing agent and its preparation method and application - Google Patents
A kind of spirit stillage decomposing agent and its preparation method and application Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/006—Waste from chemical processing of material, e.g. diestillation, roasting, cooking
- C05F5/008—Waste from biochemical processing of material, e.g. fermentation, breweries
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract
The invention discloses a kind of spirit stillage decomposing agent, compounding will be learned with high temperature resistant, ethanol-tolerant, the thermoactinomyces sacchari CP of acid and alkali-resistance characteristic and existing Cordycepps of becoming thoroughly decomposed, the synergy between bacterium of being become thoroughly decomposed using difference to compositions such as the cellulose in vinasse, lignin, starch, protein, fat while fully degraded.The drop alcoholic strength of vinasse need not be carried out, the fresh spirit stillage of direct compost fermentation by the treatment such as deacidification degree, reduce treatment process, manpower is saved, material resources, financial resources and time, into the megathermal period, short (1d reaches 55 DEG C, 3d reaches 85 DEG C), maintain the hot fermentation time (83 85 DEG C of maintenance 3d) long, shorten fermentation period (only 15 16d), shorten 26 27 days than existing spirit stillage decomposing agent, effectively increase the yield and quality (rotten degree) of organic fertilizer, prevent the secondary pollution of vinasse corrupt and alkalization of soils, protect environment and soil, for the direct During High-Temperature Composting of spirit stillage become thoroughly decomposed making high-quality organic fertilizer open a new shortcut.
Description
Technical field
The present invention relates to vinasse decomposing agent, more particularly to a kind of spirit stillage decomposing agent and its preparation method and application.
Background technology
Vinasse are the direct leftover bits and pieces during wine brewing, and current China needs vinasse to be processed up to more than 50,000,000 tons, because
This, it is concerned always for vinasse process problem after fermentation.Spirit stillage, easily ferments corrupt, distributes stench easy
Environment is polluted.Because it contains the compositions such as a large amount of crude fat, Crude starch, crude protein and abundant N P and K and polysaccharide,
It is fabulous organic fertilizer resource.Therefore organic fertilizer is made using vinasse, environmental issue can be solved, turned waste into wealth, can be again
Green agriculture production provides high-quality organic fertilizer, the usage amount of chemical fertilizer is reduced, with economic benefit higher, environmental benefit and society
Can benefit.
It is the topmost mode of current vinasse making organic fertilizer that vinasse During High-Temperature Composting is become thoroughly decomposed, and vinasse decomposing agent is vinasse
The critical materials of organic fertilizer manufacturing process, is the important leverage of final product quality and benefit, but, spirit stillage is different from other
The key character of organic fertilizer fermentation matrix is：Fresh vinasse water content big (60-65%), acidity (pH value 3.4-4.0) high, wine
High precision (6-10%), the growth and breeding of the bacterium that is unfavorable for typically becoming thoroughly decomposed, it is difficult to direct fermentation is become thoroughly decomposed.At present, related white wine
The patent document of vinasse decomposing agent is more：
The A of Chinese patent CN 106146105 disclose a kind of cassava grain stillage organic fertilizer containing amino acid, and the organic fertilizer includes
The raw material of following mass fraction：Cassava grain stillage 800-900 parts, plant ash 175-250 parts, vegetable protein 50-100 parts, compound bacteria
Plant 0.2-0.4 parts；By heat-resistant bacillus and plum, orchid streptomycete presses 1 to the composite bacteria long:The mass ratio composition of 1-1.2.
The B of Chinese patent CN 104151042 disclose a kind of vinasse decomposing agent preparation method, the master of described decomposing microbial inoculum
It is the not sea of CGMCCNo.8268 to be configured to the bacillus amyloliquefaciens and deposit number that deposit number is CGMCC No.8143
Prestige bacillus；Its compound proportion is 3:7.Effective bacterium work >=2 × 10 of decomposing agent microbial inoculum8cfu/g。
The A of Chinese patent CN 106278526 disclose a kind of preparation method of microorganism-decomposing agent, and described high temperature becomes thoroughly decomposed
Agent is liquid microbial inoculum, and the viable bacteria content of the liquid microbial inoculum is hundred million/ml of 1.8-2.5；The strain of the liquid microbial inoculum includes withered grass
Bacillus, actinomyces and saccharomycete, the bacillus subtilis, actinomyces, the strain quantity ratio of saccharomycete are 3:4:3, institute
The weight for stating the addition of high temperature decomposing agent adjusts the 0.4-0.6% of the gross weight after moisture and pH value for fertilizer matrix.
The A of Chinese patent CN 104909854 disclose a kind of sauce incense liquor grain decomposition agent strain and constitute, the decomposing microbial inoculum
It is composited by bacillus, saccharomycete, actinomyces and acetobacter, but not specific open compound proportion, its decomposing agent
Effective bacterium work >=0.5 × 10 of microbial inoculum8cfu/g。
Spirit stillage high temperature decomposing microbial inoculum disclosed above is although more, but is required for carrying out the drop alcoholic strength of vinasse, drop
Acidity, addition aid nutrition are pre-processed into grading mode, complex procedures, waste of manpower, material resources, financial resources and time, and also meeting
Causing the decomposition of vinasse does not cause thoroughly secondary pollution corrupt, while the addition that there is decomposing agent is higher, fermentation rate is low, high
The warm compost maturity cycle is long, and organic matter degradation rate is low, the defect such as not exclusively of becoming thoroughly decomposed, therefore it provides one kind need not to carry out vinasse pre-
Treatment and composition adjustment, it is art technology that can directly be inoculated with spirit stillage and carry out the quick composting microbial inoculum that During High-Temperature Composting becomes thoroughly decomposed
Personnel in the urgent need to.
The content of the invention
Technical problem solved by the invention is the shortcoming for overcoming existing spirit stillage decomposing agent, according to spirit stillage into
It is grouped into and nutritive peculiarity, by with high temperature resistant, ethanol-tolerant, the thermoactinomyces sacchari CP of acid and alkali-resistance characteristic and existing Cordycepps of becoming thoroughly decomposed
Learn compounding, there is provided one kind need not carry out vinasse deacidification, pre-process alcohol etc., energy is quick, directly become thoroughly decomposed fresh spirit stillage
Decomposing agent.
In order to achieve the above object, the present invention uses following technical scheme：
A kind of spirit stillage decomposing agent, is mainly prepared by the raw material of following parts by weight：
1-10 parts of thermoactinomyces sacchari microbial inoculum, bacillus licheniformis agent 1-5 parts, bacillus subtilis microbial agent 1-5 parts, solution
1-5 parts of bacillus amyloliquefacienses microbial inoculum；
Preferably, the spirit stillage decomposing agent, is mainly prepared by the raw material of following parts by weight：
3-7 parts of thermoactinomyces sacchari microbial inoculum, bacillus licheniformis agent 2-4 parts, bacillus subtilis microbial agent 2-4 parts, solution
2-4 parts of bacillus amyloliquefacienses microbial inoculum；
It is highly preferred that the spirit stillage decomposing agent, is mainly prepared by the raw material of following parts by weight：
5 parts of thermoactinomyces sacchari microbial inoculum, 3 parts of bacillus licheniformis agent, 3 parts of bacillus subtilis microbial agent solves starch bud
3 parts of spore bacillus microbial inoculum；
The thermoactinomyces sacchari is specially thermoactinomyces sacchari (Laceyella sacchari) CP, is from Tang of Hebei province
Mountain city cow dung high temperature become thoroughly decomposed separate in thing, purifying, screening are obtained.It is micro- that the bacterial strain is preserved in China on March 24th, 2017
(abbreviation CGMCC, address is biological inoculum preservation administration committee common micro-organisms center：BeiChen West Road, Chaoyang District, BeiJing City 1
No. 3 Institute of Microorganism, Academia Sinica of institute, postcode：100101), deposit number is CGMCC NO.13928, classification life
Entitled thermoactinomyces sacchari Laceyella sacchari；
Thermoactinomyces sacchari (Laceyella sacchari) CP can grow in the range of 30-75 DEG C, the most suitable growth
Temperature is 60 DEG C；Various digestive enzymes such as protease, lipase can be produced；
Thermoactinomyces sacchari (Laceyella sacchari) CP is the various concentrations of 1-13% in alcoholic strength (V/V)
NA fluid nutrient mediums in be coated with 50 DEG C of culture 3d in NA flat boards and NA fluid nutrient medium test tubes after 50 DEG C of culture 3d, can grow
Well, two experiments prove the tolerable alcoholic strength scope of the bacterial strain in 1-13%, with stronger alcoholic strength tolerance level；
Thermoactinomyces sacchari (Laceyella sacchari) CP is the NA liquid of the different pH values of 3-12 in pH value
50 DEG C of culture 3d, energy well-grown in NA flat boards and NA fluid nutrient medium test tubes are coated with body culture medium after 50 DEG C of culture 3d,
Two experiments prove that the scope of the tolerable pH value of the bacterial strain is 3-12, more wide in range, with stronger soda acid tolerance level.
Preferably, viable bacteria content >=5 × 10 of the thermoactinomyces sacchari microbial inoculum11cfu/g；
It is highly preferred that the preparation method of the thermoactinomyces sacchari microbial inoculum, comprises the following steps：By thermoactinomyces sacchari CP
Slant strains transfer needle is inoculated in NA seeds bottle solid medium, 58-62 DEG C of culture 22-26h, and by the aseptic washing of thalline
De- to scrape, adjustment cell concentration is 5 × 1011Cfu/mL, draws 100mL bacterium solutions and is inoculated into 0.8-1.2kg solid mediums A,
58-62 DEG C of culture 45-50h, dries 22-26h under the conditions of 55-65 DEG C, is crushed with pulverizer, crosses 40 mesh sieves and obtains final product sugarcane
The uncommon Salmonella CP microbial inoculums of orchid, wherein thermoactinomyces sacchari viable bacteria content are 5-7 × 1011cfu/g；
The preparation method of NA seeds bottle solid medium is：Take peptone 10g, powdered beef 3g, sodium chloride 10g, fine jade
Fat 20g, distilled water 1L, uniform mixing, fully dissolving, adjustment pH value is 7.0, is dispensed into after boiling in seed bottle, every bottle of 80-
100mL, 121 DEG C of sterilizing 30min, is put into inclined-plane and obtains final product；
The preparation method of the solid medium A is：Take rice husk 100g, wheat bran 300g, corn flour 200g, beancake powder
100g, starch 100g, laterite 100g, calcium sulfate 5g, calcium oxide 7.5g, dipotassium hydrogen phosphate 3g, magnesium sulfate 2g, water 400mL, uniformly
Mixing, 121 DEG C of sterilizing 45min are obtained final product.
Further, the bacillus licheniformis is bacillus licheniformis ATCC 27811, bacillus licheniformis CICC
10037th, in bacillus licheniformis CICC 10181, bacillus licheniformis CICC 10831, bacillus licheniformis CICC 10291
Any one or a few；
Preferably, the bacillus licheniformis is bacillus licheniformis ATCC 27811；
Preferably, viable bacteria content >=3 × 10 of the bacillus licheniformis agent11cfu/g；
It is highly preferred that the preparation method of the bacillus licheniformis agent, comprises the following steps：Bacillus licheniformis is oblique
Face strain transfer needle is inoculated into NA seeds bottle culture medium, and 1d is cultivated at 50 DEG C, and thalline is scraped with aseptic water elution, adjusts
Whole cell concentration is 5 × 1011Cfu/mL, draws 100mL bacterium solutions and is inoculated into 1kg solid mediums B, 50 DEG C of culture 2d, 50
Crushed with pulverizer after 2d is dried under the conditions of DEG C, cross 40 mesh sieves and obtain final product bacillus licheniformis agent, wherein lichens gemma bar
Bacterium viable bacteria content is 5 × 1011cfu/g；
The preparation method of the solid medium B is：Wheat bran 800g, powdered rice hulls 100g, beancake powder 5g, ammonium sulfate 5g are taken,
Magnesium sulfate 1g, manganese sulfate 0.5g, water 1200mL, uniform mixing, 121 DEG C of sterilizing 45min are obtained final product.
Further, the bacillus subtilis is bacillus subtilis ATCC 6051, bacillus subtilis CICC
10088th, in bacillus subtilis CICC 10090, bacillus subtilis CICC 10153, bacillus subtilis CICC 10157
Any one or a few；
Preferably, the bacillus subtilis is bacillus subtilis ATCC 6051；
Preferably, bacillus subtilis microbial agent viable bacteria content >=4 × 1010cfu/g；
It is highly preferred that the preparation method of the bacillus subtilis microbial agent, comprises the following steps：Bacillus subtilis is oblique
Face strain transfer needle is inoculated into NA seeds bottle culture medium, and 1d is cultivated at 37 DEG C, and thalline is scraped with aseptic water elution, adjusts
Whole cell concentration is 5 × 1011Cfu/mL, draws 100mL bacterium solutions and is inoculated into 1kg solid mediums C, 37 DEG C of culture 2d, 37
Crushed with pulverizer after 3d is dried under the conditions of DEG C, cross 40 mesh sieves and obtain final product bacillus subtilis microbial agent, wherein bacillus subtilis
Bacterium viable bacteria content is 6 × 1010cfu/g；
The preparation method of the solid medium C is：Take wheat bran 800g, rice husk 100g, corn flour 50g, dregs of beans 50g, sulphur
Sour magnesium 0.5g, ammonium sulfate 5g, water 1100mL, uniform mixing, 121 DEG C of sterilizing 45min are obtained final product.
Further, the bacillus amyloliquefaciens are bacillus amyloliquefaciens CICC 10035, bacillus amyloliquefaciens
CICC 10036, bacillus amyloliquefaciens CICC 10074, bacillus amyloliquefaciens CICC 10163, bacillus amyloliquefaciens
Any one or a few in CICC 20027；
Preferably, the bacillus amyloliquefaciens are bacillus amyloliquefaciens CICC 10035；
Preferably, viable bacteria content >=3 × 10 of the bacillus amyloliquefaciens microbial inoculum11cfu/g；
It is highly preferred that the preparation method of the bacillus amyloliquefaciens microbial inoculum, comprises the following steps：Starch gemma bar will be solved
Bacterium slant strains transfer needle is inoculated into NA seeds bottle culture medium, 1d is cultivated at 37 DEG C, and thalline is scraped with aseptic water elution
Under, adjustment cell concentration is 5 × 1011Cfu/mL, draws 100mL bacterium solutions and is inoculated into 1kg solid mediums D, 37 DEG C of culture 2d,
37 DEG C dry 3d after crushed with pulverizer, cross 40 mesh sieves obtain final product bacillus amyloliquefaciens microbial inoculum, wherein solution starch gemma bar
Bacterium viable bacteria content 5 × 1011cfu/g；
The preparation method of the solid medium D is：Take groundnut meal 500g, wheat bran 350g, cotton dregs 120g, peptone
3g, glucose 2g, magnesium sulfate 0.2g, water 450mL, uniform mixing, 121 DEG C of sterilizing 45min are obtained final product.
Another object of the present invention is to provide the preparation method of above-mentioned spirit stillage decomposing agent, comprises the following steps：According to matching somebody with somebody
Side, precise thermoactinomyces sacchari microbial inoculum, bacillus licheniformis agent, bacillus subtilis microbial agent, bacillus amyloliquefaciens
Microbial inoculum, spirit stillage decomposing agent is obtained final product according to equal increments method is well mixed.
Present invention also offers application of the above-mentioned spirit stillage decomposing agent in terms of quick composting spirit stillage, by the present invention
The spirit stillage decomposing agent of preparation is become thoroughly decomposed in accessing fresh spirit stillage heap body with 1~1.5 ‰ inoculum concentration in mass ratio
Complete compost maturity, reaches innoxious standard by treatment, 16d, obtains up-to-standard lees organic fertilizer.
Beneficial effect：
Spirit stillage decomposing agent prepared by the present invention, will be with resistance to height according to the constituent structure and nutritive peculiarity of spirit stillage
Temperature, ethanol-tolerant, the thermoactinomyces sacchari CP of acid and alkali-resistance characteristic learn the association between compounding, bacterium of being become thoroughly decomposed using difference with existing Cordycepps of becoming thoroughly decomposed
Same-action can fully be degraded to compositions such as the cellulose in vinasse, lignin, starch, protein, fat simultaneously, can be had
Effect improves degradation capability of the decomposing agent to vinasse.Particularly thermoactinomyces sacchari CP excellent ethanol-tolerant, acid and alkali-resistance characteristic, without
The fresh spirit stillage of direct compost fermentation by drop alcoholic strength, deacidification degree of vinasse etc. are processed is carried out, treatment process is reduced, saved
About human and material resources, financial resources and time, into the megathermal period short (1d reaches 55 DEG C, and 3d reaches 85 DEG C), maintains the hot fermentation time
(83-85 DEG C of maintenance 3d) long, shortens fermentation period (only 15-16d), and 26-27 days are shortened than existing spirit stillage decomposing agent,
Effectively increase the yield and quality (rotten degree) of organic fertilizer, it is therefore prevented that the secondary pollution corruption and alkalization of soils of vinasse, protect
Protected environment and soil, be the direct During High-Temperature Composting of spirit stillage become thoroughly decomposed making high-quality organic fertilizer open a new victory
Footpath.
Brief description of the drawings
Fig. 1 is the change of the different lower temperature for the treatment of of becoming thoroughly decomposed in spirit stillage composting process；
Fig. 2 is the change of the different lower pH value for the treatment of of becoming thoroughly decomposed in spirit stillage composting process；
Fig. 3 is the change of the different lower contents of organic matter for the treatment of of becoming thoroughly decomposed in spirit stillage composting process；
Fig. 4 is the change of the different lower total nitrogen contents for the treatment of of becoming thoroughly decomposed in spirit stillage composting process；
Fig. 5 is the change of the different lower moistures for the treatment of of becoming thoroughly decomposed in spirit stillage composting process.
Specific embodiment
The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention
It is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, it is not intended to limit the present invention
Scope, the spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, without departing substantially from this
On the premise of invention spirit and scope, the various changes that are carried out to the material component and consumption in these embodiments or change
Belong to protection scope of the present invention.
Embodiment 1
A kind of spirit stillage decomposing agent, is mainly prepared by the raw material of following parts by weight：
5 parts of thermoactinomyces sacchari microbial inoculum, 3 parts of bacillus licheniformis agent, 3 parts of bacillus subtilis microbial agent solves starch bud
3 parts of spore bacillus microbial inoculum；
The thermoactinomyces sacchari is specially thermoactinomyces sacchari (Laceyella sacchari) CP, and deposit number is
CGMCC NO.13928, Classification And Nomenclature is thermoactinomyces sacchari Laceyella sacchari；
The preparation method of the thermoactinomyces sacchari microbial inoculum, comprises the following steps：By thermoactinomyces sacchari CP slant strains
NA seeds bottle solid medium, 60 DEG C of culture 24h are inoculated in transfer needle, and thalline is scraped with aseptic water elution, adjust bacterium
Bulk concentration is 5 × 1011Cfu/mL, draws 100mL bacterium solutions and is inoculated into 1kg solid mediums A, 60 DEG C of culture 48h, in 60 DEG C of bars
24h is dried under part, is crushed with pulverizer, crossed 40 mesh sieves and obtain final product thermoactinomyces sacchari microbial inoculum, wherein thermoactinomyces sacchari is lived
Bacterial content is 7 × 1011cfu/g；
The preparation method of NA seeds bottle solid medium is：Take peptone 10g, powdered beef 3g, sodium chloride 10g, fine jade
Fat 20g, distilled water 1L, uniform mixing, fully dissolving, adjustment pH value is 7.0, is dispensed into after boiling in seed bottle, every bottle of 80-
100mL, 121 DEG C of sterilizing 30min, is put into inclined-plane and obtains final product；
The preparation method of the solid medium A is：Take rice husk 100g, wheat bran 300g, corn flour 200g, beancake powder
100g, starch 100g, laterite 100g, calcium sulfate 5g, calcium oxide 7.5g, dipotassium hydrogen phosphate 3g, magnesium sulfate 2g, water 400mL, uniformly
Mixing, 121 DEG C of sterilizing 45min are obtained final product.
The bacillus licheniformis is bacillus licheniformis ATCC 27811；
The preparation method of the bacillus licheniformis agent, comprises the following steps：Bacillus licheniformis slant strains are used
Transfer needle is inoculated into NA seeds bottle culture medium, and 1d is cultivated at 50 DEG C, and thalline is scraped with aseptic water elution, and adjustment thalline is dense
Spend is 5 × 1011Cfu/mL, draws 100mL bacterium solutions and is inoculated into 1kg solid mediums B, 50 DEG C of culture 2d, under the conditions of 50 DEG C
Crushed with pulverizer after drying 2d, cross 40 mesh sieves and obtain final product bacillus licheniformis agent, wherein bacillus licheniformis viable bacteria is contained
Measure is 5 × 1011cfu/g；
The preparation method of the solid medium B is：Wheat bran 800g, powdered rice hulls 100g, beancake powder 5g, ammonium sulfate 5g are taken,
Magnesium sulfate 1g, manganese sulfate 0.5g, water 1200mL, uniform mixing, 121 DEG C of sterilizing 45min are obtained final product.
The bacillus subtilis is bacillus subtilis ATCC 6051；
The preparation method of the bacillus subtilis microbial agent, comprises the following steps：Bacillus subtilis slant strains are used
Transfer needle is inoculated into NA seeds bottle culture medium, and 1d is cultivated at 37 DEG C, and thalline is scraped with aseptic water elution, and adjustment thalline is dense
Spend is 5 × 1011Cfu/mL, draws 100mL bacterium solutions and is inoculated into 1kg solid mediums C, 37 DEG C of culture 2d, under the conditions of 37 DEG C
Crushed with pulverizer after drying 3d, cross 40 mesh sieves and obtain final product bacillus subtilis microbial agent, wherein bacillus subtilis viable bacteria is contained
Measure is 6 × 1010cfu/g；
The preparation method of the solid medium C is：Take wheat bran 800g, rice husk 100g, corn flour 50g, dregs of beans 50g, sulphur
Sour magnesium 0.5g, ammonium sulfate 5g, water 1100mL, uniform mixing, 121 DEG C of sterilizing 45min are obtained final product.
The bacillus amyloliquefaciens are bacillus amyloliquefaciens CICC 10035；
The preparation method of the bacillus amyloliquefaciens microbial inoculum, comprises the following steps：By bacillus amyloliquefaciens inclined-plane bacterium
Kind transfer needle is inoculated into NA seeds bottle culture medium, and 1d is cultivated at 37 DEG C, and thalline is scraped with aseptic water elution, adjusts bacterium
Bulk concentration is 5 × 1011Cfu/mL, draws 100mL bacterium solutions and is inoculated into 1kg solid mediums D, 37 DEG C of culture 2d, 37 DEG C of dryings
Crushed with pulverizer after 3d, cross 40 mesh sieves and obtain final product bacillus amyloliquefaciens microbial inoculum, wherein bacillus amyloliquefaciens viable bacteria is contained
Amount 5 × 1011cfu/g；
The preparation method of the solid medium D is：Take groundnut meal 500g, wheat bran 350g, cotton dregs 120g, peptone
3g, glucose 2g, magnesium sulfate 0.2g, water 450mL, uniform mixing, 121 DEG C of sterilizing 45min are obtained final product.
Preparation method：According to formula, precise thermoactinomyces sacchari microbial inoculum, bacillus licheniformis agent, withered grass gemma
Bacillus microbial inoculum, bacillus amyloliquefaciens microbial inoculum obtains final product spirit stillage decomposing agent according to equal increments method is well mixed.
Embodiment 2
A kind of spirit stillage decomposing agent, is mainly prepared by the raw material of following parts by weight：
3 parts of thermoactinomyces sacchari microbial inoculum, 2 parts of bacillus licheniformis agent, 2 parts of bacillus subtilis microbial agent solves starch bud
2 parts of spore bacillus microbial inoculum；
The thermoactinomyces sacchari is specially thermoactinomyces sacchari (Laceyella sacchari) CP, and deposit number is
CGMCC NO.13928, Classification And Nomenclature is thermoactinomyces sacchari Laceyella sacchari；
The preparation method of the thermoactinomyces sacchari microbial inoculum, comprises the following steps：By thermoactinomyces sacchari CP slant strains
NA seeds bottle solid medium, 58 DEG C of culture 22h are inoculated in transfer needle, and thalline is scraped with aseptic water elution, adjust bacterium
Bulk concentration is 5 × 1011Cfu/mL, draws 100mL bacterium solutions and is inoculated into 0.8kg solid mediums A, 58 DEG C of culture 45h, at 55 DEG C
Under the conditions of dry 22h, crushed with pulverizer, cross 40 mesh sieves obtain final product thermoactinomyces sacchari microbial inoculum, wherein thermoactinomyces sacchari
Viable bacteria content is 6 × 1011cfu/g；
The bacillus licheniformis is bacillus licheniformis CICC 10037；
The bacillus subtilis is bacillus subtilis CICC 10088；
The bacillus amyloliquefaciens are bacillus amyloliquefaciens CICC 10036；
The bacillus licheniformis agent, bacillus subtilis microbial agent, the preparation method of bacillus amyloliquefaciens microbial inoculum and
Viable bacteria content is with embodiment 1；
It is NA seeds bottle solid medium, solid medium A, solid medium B, solid medium C, solid in preparation process
The preparation method of body culture medium D is with embodiment 1；
The spirit stillage decomposing agent preparation method is with embodiment 1.
Embodiment 3
A kind of spirit stillage decomposing agent, is mainly prepared by the raw material of following parts by weight：
7 parts of thermoactinomyces sacchari microbial inoculum, 4 parts of bacillus licheniformis agent, 4 parts of bacillus subtilis microbial agent solves starch bud
4 parts of spore bacillus microbial inoculum；
The thermoactinomyces sacchari is specially thermoactinomyces sacchari (Laceyella sacchari) CP, and deposit number is
CGMCC NO.13928, Classification And Nomenclature is thermoactinomyces sacchari Laceyella sacchari；
The preparation method of the thermoactinomyces sacchari microbial inoculum, comprises the following steps：By thermoactinomyces sacchari CP slant strains
NA seeds bottle solid medium, 62 DEG C of culture 26h are inoculated in transfer needle, and thalline is scraped with aseptic water elution, adjust bacterium
Bulk concentration is 5 × 1011Cfu/mL, draws 100mL bacterium solutions and is inoculated into 1.2kg solid mediums A, 62 DEG C of culture 50h, at 65 DEG C
Under the conditions of dry 26h, crushed with pulverizer, cross 40 mesh sieves obtain final product thermoactinomyces sacchari microbial inoculum, wherein thermoactinomyces sacchari
Viable bacteria content is 5 × 1011cfu/g；
The bacillus licheniformis is bacillus licheniformis CICC 10181；
The bacillus subtilis is bacillus subtilis CICC 10090；
The bacillus amyloliquefaciens are bacillus amyloliquefaciens CICC 10074；
The bacillus licheniformis agent, bacillus subtilis microbial agent, the preparation method of bacillus amyloliquefaciens microbial inoculum and
Viable bacteria content is with embodiment 1；
It is NA seeds bottle solid medium, solid medium A, solid medium B, solid medium C, solid in preparation process
The preparation method of body culture medium D is with embodiment 1；
The spirit stillage decomposing agent preparation method is with embodiment 1.
Embodiment 4
A kind of spirit stillage decomposing agent, is mainly prepared by the raw material of following parts by weight：
1 part of thermoactinomyces sacchari microbial inoculum, 1 part of bacillus licheniformis agent, 1 part of bacillus subtilis microbial agent solves starch bud
1 part of spore bacillus microbial inoculum；
The thermoactinomyces sacchari is specially thermoactinomyces sacchari (Laceyella sacchari) CP, and deposit number is
CGMCC NO.13928, Classification And Nomenclature is thermoactinomyces sacchari Laceyella sacchari；
The preparation method of the thermoactinomyces sacchari microbial inoculum, comprises the following steps：By thermoactinomyces sacchari CP slant strains
NA seeds bottle solid medium, 58 DEG C of culture 26h are inoculated in transfer needle, and thalline is scraped with aseptic water elution, adjust bacterium
Bulk concentration is 5 × 1011Cfu/mL, draws 100mL bacterium solutions and is inoculated into 0.8kg solid mediums A, 62 DEG C of culture 45h, at 65 DEG C
Under the conditions of dry 22h, crushed with pulverizer, cross 40 mesh sieves obtain final product thermoactinomyces sacchari microbial inoculum, wherein thermoactinomyces sacchari
Viable bacteria content is 6.2 × 1011cfu/g；
The bacillus licheniformis is bacillus licheniformis CICC 10831；
The bacillus subtilis is bacillus subtilis CICC 10153；
The bacillus amyloliquefaciens are bacillus amyloliquefaciens CICC 10163；
The bacillus licheniformis agent, bacillus subtilis microbial agent, the preparation method of bacillus amyloliquefaciens microbial inoculum and
Viable bacteria content is with embodiment 1；
It is NA seeds bottle solid medium, solid medium A, solid medium B, solid medium C, solid in preparation process
The preparation method of body culture medium D is with embodiment 1；
The spirit stillage decomposing agent preparation method is with embodiment 1.
Embodiment 5
A kind of spirit stillage decomposing agent, is mainly prepared by the raw material of following parts by weight：
10 parts of thermoactinomyces sacchari microbial inoculum, 5 parts of bacillus licheniformis agent, 5 parts of bacillus subtilis microbial agent solves starch bud
5 parts of spore bacillus microbial inoculum；
The thermoactinomyces sacchari is specially thermoactinomyces sacchari (Laceyella sacchari) CP, and deposit number is
CGMCC NO.13928, Classification And Nomenclature is thermoactinomyces sacchari Laceyella sacchari；
The preparation method of the thermoactinomyces sacchari microbial inoculum, comprises the following steps：By thermoactinomyces sacchari CP slant strains
NA seeds bottle solid medium, 62 DEG C of culture 22h are inoculated in transfer needle, and thalline is scraped with aseptic water elution, adjust bacterium
Bulk concentration is 5 × 1011Cfu/mL, draws 100mL bacterium solutions and is inoculated into 1.2kg solid mediums A, 58 DEG C of culture 50h, at 55 DEG C
Under the conditions of dry 26h, crushed with pulverizer, cross 40 mesh sieves obtain final product thermoactinomyces sacchari microbial inoculum, wherein thermoactinomyces sacchari
Viable bacteria content is 5.6 × 1011cfu/g；
The bacillus licheniformis is bacillus licheniformis ATCC 27811 and bacillus licheniformis CICC 10291；
The bacillus subtilis is bacillus subtilis ATCC 6051, bacillus subtilis CICC 10153 and withered grass
Bacillus CICC 10157；
The bacillus amyloliquefaciens are bacillus amyloliquefaciens CICC 10036, bacillus amyloliquefaciens CICC
10074th, bacillus amyloliquefaciens CICC 10163 and bacillus amyloliquefaciens CICC 20027；
The bacillus licheniformis agent, bacillus subtilis microbial agent, the preparation method of bacillus amyloliquefaciens microbial inoculum and
Viable bacteria content is with embodiment 1；
It is NA seeds bottle solid medium, solid medium A, solid medium B, solid medium C, solid in preparation process
The preparation method of body culture medium D is with embodiment 1；
The bacillus licheniformis agent is by the microbial inoculums of bacillus licheniformis ATCC 27811 and bacillus licheniformis CICC
10291 microbial inoculums in mass ratio 2:1 uniform mixing；
The bacillus subtilis microbial agent is by the microbial inoculums of bacillus subtilis ATCC 6051, bacillus subtilis CICC
10153 microbial inoculums and the microbial inoculums of bacillus subtilis CICC 10157 in mass ratio 1:1:1 uniform mixing；
The bacillus amyloliquefaciens microbial inoculum is by the microbial inoculums of bacillus amyloliquefaciens CICC 10036, bacillus amyloliquefaciens
The microbial inoculums of CICC 10074, the microbial inoculums of bacillus amyloliquefaciens CICC 10163 and the microbial inoculums of bacillus amyloliquefaciens CICC 20027 press matter
Amount compares 4:3:2:1 uniform mixing；
The spirit stillage decomposing agent preparation method is with embodiment 1.
Embodiment 6
A kind of spirit stillage decomposing agent, is mainly prepared by the raw material of following parts by weight：
5 parts of thermoactinomyces sacchari microbial inoculum, 3 parts of bacillus licheniformis agent, 3 parts of bacillus subtilis microbial agent solves starch bud
3 parts of spore bacillus microbial inoculum；
The thermoactinomyces sacchari is specially thermoactinomyces sacchari (Laceyella sacchari) CP, and deposit number is
CGMCC NO.13928, Classification And Nomenclature is thermoactinomyces sacchari Laceyella sacchari；
The preparation method and viable bacteria content of the thermoactinomyces sacchari microbial inoculum are with embodiment 1；
It is NA seeds bottle solid medium, solid medium A, solid medium B, solid medium C, solid in preparation process
The preparation method of body culture medium D is with embodiment 1；
The bacillus licheniformis viable bacteria content is 3 × 1011cfu/g；
The bacillus subtilis viable bacteria content is 4 × 1010cfu/g；
The bacillus amyloliquefaciens viable bacteria content 3 × 1011cfu/g；
The preparation method of the spirit stillage decomposing agent is with embodiment 1.
The thermoactinomyces sacchari of embodiment 7 (Laceyella sacchari) CP pH value tolerance tests
The appropriate thalline of thermoactinomyces sacchari CP slant strains pickings is accessed into 100mL NA fluid nutrient mediums, 50 DEG C, 200r/
Min shaking table cultures 24h obtains seed liquor, is accessed with 1 ‰ inoculum concentrations be tuned into pH value as 1.5 respectively first, and 2,2.5,3,3.5,4,
In 4.5,5,5.5,6,6.5,7,7.5,8,8.5,9,9.5,10,10.5,11,11.5,12 NA fluid nutrient mediums, 50 DEG C,
NA flat boards are coated with after 200r/min shaking table culture cultures 3d, growing state is observed；Then seed liquor is accessed with 1 ‰ inoculum concentrations again
The NA fluid nutrient medium test tubes of same pH gradient, 50 DEG C, 200r/min shaking table culture 3d observe turbidity.
Result of the test：Thermoactinomyces sacchari CP 50 DEG C of culture 3d in the NA fluid nutrient mediums of pH value 3-12, coating NA is flat
Plate can well-grown；The well-grown after 50 DEG C of NA fluid nutrient mediums test tube, 200r/min shaking table cultures 3d, turbidity is bright
Aobvious, two experiments prove the tolerable pH scopes of thermoactinomyces sacchari CP in 3-12, with acid-base value tolerance higher.
The thermoactinomyces sacchari of embodiment 8 (Laceyella sacchari) CP ethanol tolerances are tested：
The appropriate bacterium of thermoactinomyces sacchari CP slant strains pickings is accessed into 100mL NA fluid nutrient mediums, 50 DEG C, 200r/
Min shaking table cultures 24h obtains seed liquor, accessed with 1 ‰ inoculum concentrations first be tuned into respectively alcoholic strength as 1%, 3%, 5%, 7%,
9%th, in 11%, 13%, 15%, 20% NA fluid nutrient mediums, 50 DEG C, 200r/min culture 3d after be coated with NA flat boards, observation
Growing state；Then seed liquor is accessed the NA fluid nutrient medium test tubes of identical alcohol concentration gradient, 50 with 1 ‰ inoculum concentrations again
DEG C, 200r/min shaking table culture 3d, observe turbidity.
Result of the test：Thermoactinomyces sacchari CP is 50 DEG C of culture 3d in the NA fluid nutrient mediums of 1-13% in alcoholic strength, is applied
Cloth NA flat boards can well-grown；The well-grown after 50 DEG C of NA fluid nutrient mediums test tube, 200r/min shaking table cultures 3d is muddy
Degree is obvious, and two experiments prove that the tolerable alcoholic strength scopes of thermoactinomyces sacchari CP are 1-13%, with alcoholic strength higher
Tolerance.
Embodiment 9 is tested using the spirit stillage compost maturity that spirit stillage decomposing agent prepared by the present invention is carried out
1. vinasse compost maturity process
Spirit stillage decomposing agent prepared by the embodiment of the present invention 1 accesses alcoholic strength with the inoculum concentration of mass ratio 1.2 ‰
During 10%, pH are 3.58 fresh spirit stillage heap body, mix thoroughly, heap body length, width and height are respectively：200m × 2m × 1.5m, initial heap
Rich water is divided into 65%, when pile temperature is more than or equal to 55 DEG C, starts to carry out first time overturning with dumper, and one is hereafter overturned per 1d
It is secondary, (treatment of becoming thoroughly decomposed above is defined as processing L)；
Two other same materials is set simultaneously, the vinasse heap body of scale becomes thoroughly decomposed treatment as control, is defined as processing CK1
And CK2, wherein treatment CK1 is not to be inoculated with decomposing agent, to access commercially available spirit stillage decomposing agent, test method exists together treatment CK2
Reason L.
2. the result of the test that treatment L and treatment CK1, treatment CK2 are contrasted：
2.1 temperature
By accompanying drawing 1 as can be seen that the compost 1d for the treatment of L can reach 55 DEG C, 3d reaches 85 DEG C of maximum temperature and in 83-
85 DEG C continue 3d, can reach compost hazard-free standard；Treatment CK2 4d reach 55 DEG C, and 8d reaches 74 DEG C of maximum temperature only to be tieed up
1d is held, the maximum temperature that treatment L reaches is higher 11 DEG C than treatment CK2；Treatment L can terminate to become thoroughly decomposed on the 16th day in fermentation, process CK2
Terminate to become thoroughly decomposed after 42d, treatment L times phase of becoming thoroughly decomposed relatively process CK2 26d reaches and becomes thoroughly decomposed in advance, treatment CK1 becomes thoroughly decomposed ability in 57d
Terminate；The above results show that inoculation decomposing agent can accelerate the degraded of organic matter in spirit stillage heap body, relative to commercially available white wine wine
Poor decomposing agent, fermentation rate is high, programming rate fast during spirit stillage quick composting agent compost maturity prepared by the addition present invention, enter
Time for entering the megathermal period is short, maintain that the time of megathermal period is long, compost maturity fermentation period is short, with significant quick, directly rotten
The characteristics of ripe fresh spirit stillage, without dropping the pretreatment such as alcoholic strength, deacidification.
2.2pH values
By accompanying drawing 2 as can be seen that in whole composting process, treatment CK1, treatment CK2 and treatment L compost later stages pH are distinguished
It is 7.74,7.62,7.42, on the whole, inoculation decomposing agent influences not substantially on heap body pH, but treatment L relatively low pH value energy
The volatilization of ammonia in composting process is reduced, so as to reduce the loss of nitrogen.
2.3 contents of organic matter
By accompanying drawing 3 as can be seen that the content of organic matter that CK1, treatment CK2 and treatment L are processed at the end of compost is respectively
61.28%th, 51.75% and 45.85%, reduce 27.15% respectively than the initial content of organic matter (88.43%), 36.68%,
42.58%；Changed from the compost content of organic matter, inoculation decomposing agent is larger to the degradation of compost organic matter, Er Qiejie
The degradation efficiency for planting spirit stillage quick composting agent prepared by the present invention is significantly higher than commercially available spirit stillage decomposing agent.
2.4 total nitrogen contents
By accompanying drawing 4 as can be seen that three treatment are compared, the heap body total nitrogen content of inoculation decomposing agent is apparently higher than control group
CK1, and spirit stillage quick composting agent prepared by the inoculation present invention is more slightly higher than the total nitrogen content of commercially available spirit stillage decomposing agent.This
When treatment L total nitrogen content be 2.23%, process CK2 total nitrogen content be 2.12%, process CK1 total nitrogen content be
2.06%.
2.5 moistures
By accompanying drawing 5 as can be seen that at the end of compost maturity, the moisture for processing L, CK2, CK1 is respectively 21.16%,
23.62%th, 25.32%, the content for processing L moisture is substantially less than treatment CK1 and treatment CK2, white wine prepared by the addition present invention
Fermentation rate is high during vinasse quick composting agent compost maturity, programming rate is fast, into the megathermal period time it is short, maintain the megathermal period
Time is long, effectively accelerate the volatilization of heap body moisture, accelerates degree of becoming thoroughly decomposed, and can effectively shorten moisture compared with commercially available decomposing agent waves
The hair time, while obtaining quality lees organic fertilizer higher.
It should be noted that spirit stillage decomposing agent prepared by embodiment of the present invention 2-6 equally has above-mentioned test effect,
Otherness is not notable between each embodiment.
Claims (10)
1. a kind of spirit stillage decomposing agent, is mainly prepared by the raw material of following parts by weight：1-10 parts of thermoactinomyces sacchari microbial inoculum,
Bacillus licheniformis agent 1-5 parts, bacillus subtilis microbial agent 1-5 parts, 1-5 parts of bacillus amyloliquefaciens microbial inoculum；
The thermoactinomyces sacchari is specially thermoactinomyces sacchari (Laceyella sacchari) CP, and deposit number is CGMCC
NO.13928。
2. spirit stillage decomposing agent as claimed in claim 1, it is characterised in that the spirit stillage decomposing agent, mainly by with
It is prepared by the raw material of lower parts by weight：3-7 parts of thermoactinomyces sacchari microbial inoculum, bacillus licheniformis agent 2-4 parts, bacillus subtilis
Microbial inoculum 2-4 parts, 2-4 parts of bacillus amyloliquefaciens microbial inoculum.
3. spirit stillage decomposing agent as claimed in claim 1, it is characterised in that the spirit stillage decomposing agent, mainly by with
It is prepared by the raw material of lower parts by weight：5 parts of thermoactinomyces sacchari microbial inoculum, 3 parts of bacillus licheniformis agent, bacillus subtilis microbial agent
3 parts, 3 parts of bacillus amyloliquefaciens microbial inoculum.
4. the spirit stillage decomposing agent as described in claim 1-3 is any, it is characterised in that the thermoactinomyces sacchari microbial inoculum
Preparation method, comprises the following steps：Thermoactinomyces sacchari CP slant strains transfer needles are inoculated in NA seeds bottle solid culture
Base, 60 DEG C of culture 24h, and thalline is scraped with aseptic water elution, adjustment cell concentration is 5 × 1011Cfu/mL, draws 100mL
Bacterium solution is inoculated into 1kg solid mediums A, 60 DEG C of culture 48h, and 24h is dried under the conditions of 60 DEG C, is crushed with pulverizer,
Cross 40 mesh sieves and obtain final product thermoactinomyces sacchari microbial inoculum；
The preparation method of NA seeds bottle solid medium is：Take peptone 10g, powdered beef 3g, sodium chloride 10g, agar
20g, distilled water 1L, uniform mixing, fully dissolving, adjustment pH value is 7.0, is dispensed into after boiling in seed bottle, every bottle of 80-
100mL, 121 DEG C of sterilizing 30min, is put into inclined-plane and obtains final product；
The preparation method of the solid medium A is：Rice husk 100g, wheat bran 300g, corn flour 200g, beancake powder 100g are taken, is formed sediment
Powder 100g, laterite 100g, calcium sulfate 5g, calcium oxide 7.5g, dipotassium hydrogen phosphate 3g, magnesium sulfate 2g, water 400mL, uniform mixing,
121 DEG C of sterilizing 45min are obtained final product.
5. the spirit stillage decomposing agent as described in claim 1-3 is any, it is characterised in that the bacillus licheniformis is lichens
Bacillus ATCC 27811, bacillus licheniformis CICC 10037, bacillus licheniformis CICC 10181, bacillus licheniformis
Any one or a few in CICC 10831, bacillus licheniformis CICC 10291.
6. the spirit stillage decomposing agent as described in claim 1-3 is any, it is characterised in that the bacillus subtilis is withered grass
Bacillus ATCC 6051, bacillus subtilis CICC 10088, bacillus subtilis CICC 10090, bacillus subtilis
Any one or a few in CICC 10153, bacillus subtilis CICC 10157.
7. the spirit stillage decomposing agent as described in claim 1-3 is any, it is characterised in that the bacillus amyloliquefaciens are solution
Bacillus amyloliquefacienses CICC 10035, bacillus amyloliquefaciens CICC 10036, bacillus amyloliquefaciens CICC 10074, Xie Dian
Any one or a few in afnyloliquefaciens CICC 10163, bacillus amyloliquefaciens CICC 20027.
8. the spirit stillage decomposing agent as described in claim 1-3 is any, it is characterised in that the thermoactinomyces sacchari microbial inoculum
Viable bacteria content >=5 × 1011cfu/g；Viable bacteria content >=3 × 10 of the bacillus licheniformis agent11cfu/g；The withered grass bud
Spore bacillus microbial inoculum viable bacteria content >=4 × 1010cfu/g；Viable bacteria content >=3 × 10 of the bacillus amyloliquefaciens microbial inoculum11cfu/
g。
9. the preparation method of the spirit stillage decomposing agent as described in claim 1-8 is any, it is characterised in that including following step
Suddenly：According to formula, precise thermoactinomyces sacchari microbial inoculum, bacillus licheniformis agent, bacillus subtilis microbial agent solves starch
Gemma bacillus agent, spirit stillage decomposing agent is obtained final product according to equal increments method is well mixed.
10. application of the spirit stillage decomposing agent as described in claim 1-8 is any in terms of quick composting spirit stillage.
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| CN110903112A (en) * | 2019-12-03 | 2020-03-24 | 威海金颐阳药业有限公司 | Organic fertilizer for American ginseng |
| CN117402020A (en) * | 2023-12-13 | 2024-01-16 | 中农金瑞肥业有限公司 | Yield-increasing biological organic fertilizer and preparation method thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108251317A (en) * | 2018-03-30 | 2018-07-06 | 陕西枫丹百丽生物科技有限公司 | One plant of saccharomycopsis fibuligera and its application |
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| CN108624539B (en) * | 2018-06-08 | 2021-10-08 | 河北木美土里科技有限公司 | Straw and cow dung ultrahigh-temperature decomposition agent and preparation and application thereof |
| CN110903112A (en) * | 2019-12-03 | 2020-03-24 | 威海金颐阳药业有限公司 | Organic fertilizer for American ginseng |
| CN117402020A (en) * | 2023-12-13 | 2024-01-16 | 中农金瑞肥业有限公司 | Yield-increasing biological organic fertilizer and preparation method thereof |
| CN117402020B (en) * | 2023-12-13 | 2024-03-15 | 中农金瑞肥业有限公司 | Yield-increasing biological organic fertilizer and preparation method thereof |
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