CN102249754A - Paenibacillus polymyxa microbial fertilizer produced through sweet potato starch waste water recovery - Google Patents
Paenibacillus polymyxa microbial fertilizer produced through sweet potato starch waste water recovery Download PDFInfo
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- CN102249754A CN102249754A CN2010101733139A CN201010173313A CN102249754A CN 102249754 A CN102249754 A CN 102249754A CN 2010101733139 A CN2010101733139 A CN 2010101733139A CN 201010173313 A CN201010173313 A CN 201010173313A CN 102249754 A CN102249754 A CN 102249754A
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- waste water
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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Abstract
The invention which relates to a method for producing a Paenibacillus polymyxa microbial fertilizer through sweet potato starch waste water recovery belongs to the technical field of microbial fertilizers and waste water recovery. The method which treats waste water in a sweet potato starch production process as a main nutrient raw material adopts a liquid state fermentation way to produce the Paenibacillus polymyxa microbial fertilizer. A production technology of the fertilizer comprises the following steps: 1, pretreating sweet potato starch waste water through adding cellulase and pectase; 2, obtaining liquid seeds through inoculating slant-preserved Paenibacillus polymyxa to a triangular flask which is provided with pretreated sweet potato starch waste water and carrying out oscillation culture; 3, obtaining liquid state fermentation products through inoculating the liquid seeds at a volume ratio of 5% to a fermentor which is provided with the pretreated sweet potato starch waste water and culturing through accessing aseptic air; and 4, carrying out bacterial counting on a cultured broth, adding a stabilizer and an assistant, and packaging. The Paenibacillus polymyxa microbial fertilizer produced through the technology has the characteristics of low production cost and environmental protection, and allows the soil environment quality to be substantially improved, the output and the quality of crops to be improved, and multiple plant diseases to be controlled. The method of the invention allows an effective recovery treatment process for effectively treating a large amount of high concentration waste water generated in the sweet potato starch production process to be found, and pollutant discharge to be reduced.
Description
Technical field:
The invention belongs to microbial fertilizer and waste water reclaiming technical field.
Background technology:
Sweet potato is the good raw material sources of Starch Production, and its deep processing can solve because of storing the improper phenomenon of mashed potato in a large number that causes of bright potato, and sweet potato starch can be produced hundreds of value products through the processing of different depths, about 10-30 times of increment.But, present complete processing, 1 ton of about output 6-15 ton of sweet potato starch of every production high concentrated organic wastewater, the COD value of these waste water is up to more than the 10000mg/L, mainly contain solvability starch, protein, pectin, organic acid and a spot of grease, easily corrupt fermentation makes the water quality blackout smelly, enters the dissolved oxygen in the river meeting consume water, measure river severe depletion of oxygen when big, therefore generation anaerobism corruption is distributed stench, and hydrocoles such as fish, shrimp, shellfish may be and death by suffocation.And adopt general sewage treatment process that the wastewater treatment of high density like this is up to standard, consume surprising.Consider that from another angle the organic matter in the sweet potato starch waste water (comprising: protein, pectin, carbohydrate etc.) is the good nutrition substrate of microorganism growth, can make microbiological culture media, the fermentative production microniological proudcts.
(Paenibacillus polymyxa) is widely distributed at occurring in nature for Paenibacillus polymyxa, is a kind of important biocontrol microorganisms, and various plants fungi, Micobial Disease are all had the better prevention effect.Wang Zhiwen etc. have reported that a strain Paenibacillus polymyxa Cp-S316 bacterial strain is to the animals and plants fungi, the bacteriosis original has resistance of wide spectrum, utilize the absorption of macroporous adsorbent resin to the antifungus active substance of many sticking class spore bacillus Cp-S316 generations, desorption performance, filter out its optimum solution vapor, this active substance is to warm, UV stable, to trypsinase, Proteinase K has certain tolerance, responsive to the chloroform part, stable to acid, to alkali sensitivity (Wang Zhiwen, Yuan Shitao, He Liang etc., the extraction of Paenibacillus polymyxa Cp-S316 antifungus active substance and part property research thereof, agricultural environment science journal, 2007,26 (4): 1464-1468).(Granted publication number: report is cultivated CN 1169942C) Paenibacillus polymyxa HY96-2 and fermented supernatant fluid thereof can be used for preventing the vegetative bacteria bacterial wilt to Chinese patent, but also can be used to prevent and treat the plant seedling blight, samping off, and cucumber fusarium axysporum, tomato wilt, the eggplant blight, root rot, and plant had fairly obvious promotion growth effect.Chinese patent (Granted publication number: disclose Paenibacillus polymyxa CN1186441C) and can be used as a kind of composition decomposing straw in the microbe fertilizer microbial inoculum, effectively agricultural waste is too much on the one hand in the solution agriculture production, polluted-water and environment, difficult treatment, chemical fertilizer on the other hand, agricultural chemicals uses too much, the contradiction of soil deterioration.(Granted publication number: disclosing Paenibacilluspolymyxa Jaas cd CN 1325635C) can enter in the plant materials under lower concentration Chinese patent, and can field planting in plant materials, breeding and conduction; Plant is had the growth of promotion simultaneously, improve output, the effect of disease resistance particularly has good prevention effect to soil-borne vascular bundle disease.
The production method of Paenibacillus polymyxa has multiple, as: (publication number: disclose a kind of production method of Paenibacillus polymyxa CN 1844360A), its substratum is bean dregs to Chinese patent.Chinese patent (Granted publication number: use cooked meat medium CN 1186441C) as the Paenibacillus polymyxa fermention medium.With sweet potato starch waste water is that the method that substratum is produced the Paenibacillus polymyxa microbial fertilizer is not seen bibliographical information.
Summary of the invention:
The purpose of this invention is to provide a kind of method of can recycling sweet potato processing waste water producing the Paenibacillus polymyxa microbial fertilizer.
Bacterial strain used in the present invention is to imitate Paenibacillus polymyxa (Paenibacillus polymyxa) the EBL-06 bacterial strain that promotes plant-growth, antagonism phytopathogen by the plant height that contriver's separation screening comes out, this bacterial strain is deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center on February 26th, 2008, deposit number is: CGMCC No.2377, depositary institution address: Datun Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode: 100101.
The production method of Paenibacillus polymyxa microbial fertilizer of the present invention is as follows:
1, sweet potato starch Wastewater Pretreatment: earlier waste water is heated to 100 ℃, boiled 3-10 minute, be cooled to 40-55 ℃, add cellulase 0-0.5IU/mL and polygalacturonase 0-15IU/mL, continuously stirring is incubated 10-60 minute, again in 115-126 ℃ of sterilization 10-35 minute.
2, liquid seeds preparation: the Paenibacillus polymyxa of inoculation slant preservation in the triangular flask that the good sweet potato starch waste water of pre-treatment is housed, in 30-37 ℃, 120-200 rev/min shaking culture 24-48 hour, obtain liquid seeds.
3, liquid state fermentation: the inoculation volume ratio is 5% liquid seeds in the good sweet potato starch wastewater fermentation jar of pre-treatment is housed, and in 25-40 ℃, logical sterile air was cultivated 12-48 hour.
4, aftertreatment: cultured fermented liquid is adjusted to 10 after bacterial count
9-10
10CFU/mL adds microbial-bacterial fertilizer stablizer commonly used and auxiliary agent, packing, and the place preserves in the cool place.
Embodiment:
The Paenibacillus polymyxa microbial fertilizer of being produced by technical solution of the present invention can promote crop to nutrient absorbing and utilization, reduce the usage quantity of chemical fertilizer, can effectively prevent and treat multiple fungi plant disease, reduce the usage quantity of agricultural chemicals, thereby improve the farm environment quality; And production cost is low, has effectively utilized waste water, reduces pollutant emission.Embodiment is as follows:
Embodiment 1: sweet potato starch waste water is produced the Paenibacillus polymyxa microbial fertilizer
The sweet potato starch Wastewater Pretreatment: earlier waste water is heated to 100 ℃, boiled 5 minutes, be cooled to 50 ℃, add cellulase 0.1IU/mL and polygalacturonase 7IU/mL, continuously stirring is incubated 30 minutes, again in 121 ℃ of sterilizations 15 minutes.
Liquid seeds preparation: the Paenibacillus polymyxa of inoculation slant preservation in the triangular flask that the good sweet potato starch waste water of pre-treatment is housed, in 32 ℃, 150 rev/mins of shaking culture 30 hours obtain liquid seeds.
Liquid state fermentation: inoculate the liquid seeds of 5% (volume ratio) in the good sweet potato starch wastewater fermentation jar of pre-treatment is housed, in 32 ℃, logical sterile air was cultivated 16 hours.
Aftertreatment: cultured fermented liquid is adjusted to 6 * 10 after bacterial count
9CFU/mL adds microbial-bacterial fertilizer stablizer commonly used and auxiliary agent, packing, and the place preserves in the cool place.
Embodiment 2: the Paenibacillus polymyxa microbial fertilizer is applied to vegetables and produces
Adopt the experiment of green house of vegetables sub-district, 12 experiment sub-districts that conditionally complete is identical are set, each sub-district 60m
2, the plantation rape in the rape seedling phase, keeps 3 sub-districts of not applying fertilizer; The Paenibacillus polymyxa microbial fertilizer is respectively being used once according to the rate of fertilizer application of 50L/ mu behind the rape complete germination He after 3 weeks in 3 sub-districts; Urea is respectively being used once according to the rate of fertilizer application of 15kg/ mu behind the rape complete germination He after 3 weeks in 3 sub-districts; 3 rates of fertilizer application according to 15kg/ mu are respectively being used urea once behind the rape complete germination He after 3 weeks, and use microbial fertilizer once, irrigation that each sub-district is identical and way to manage according to the rate of fertilizer application of 50L/ mu in 1 day after using urea at every turn.Growth of rape 5 all " Invest, Then Investigate " yield and qualities.Use microbial fertilizer separately and use the yield of rape basically identical of urea separately, all than the sub-district volume increase about 70% of not applying fertilizer, the ratio that two kinds of fertilizer is all used is only used a kind volume increase about 50%, use in the rape of urea nitrate and nitrite content separately and exceed 50% and more than 20 times than the rape that do not apply fertilizer respectively, and use separately in the rape that microbial fertilizer and two kinds of fertilizer all uses in nitrate and the nitrite content and the rape that do not apply fertilizer quite.Show the existing good effect of increasing production of this microbial fertilizer, can obviously improve the quality of rape again.
Claims (1)
1. method of utilizing sweet potato starch waste water to produce Paenibacillus polymyxa (Paenibacillus polymyxa) microbial fertilizer, it is characterized in that, this method is to be that the main medium raw material carries out liquid state fermentation and produces the Paenibacillus polymyxa microbial fertilizer with the waste water that produces in the sweet potato starch production process, comprises the steps:
(1) sweet potato starch Wastewater Pretreatment: earlier waste water is heated to 100 ℃, boiled 3-10 minute, be cooled to 40-55 ℃, add cellulase 0-0.5IU/mL and polygalacturonase 0-15IU/mL, continuously stirring is incubated 10-60 minute, again in 115-126 ℃ of sterilization 10-35 minute;
(2) liquid seeds preparation: the Paenibacillus polymyxa of inoculation slant preservation in the triangular flask that the good sweet potato starch waste water of pre-treatment is housed, in 30-37 ℃, 120-200 rev/min shaking culture 24-48 hour, obtain liquid seeds;
(3) liquid state fermentation: the inoculation volume ratio is 5% liquid seeds in the good sweet potato starch wastewater fermentation jar of pre-treatment is housed, and in 25-40 ℃, logical sterile air was cultivated 12-48 hour;
(4) aftertreatment: cultured fermented liquid is adjusted to 10 after bacterial count
9-10
10CFU/mL adds microbial-bacterial fertilizer stablizer commonly used and auxiliary agent, packing, and the place preserves in the cool place.
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| CN2010101733139A CN102249754A (en) | 2010-05-17 | 2010-05-17 | Paenibacillus polymyxa microbial fertilizer produced through sweet potato starch waste water recovery |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102703363A (en) * | 2012-05-14 | 2012-10-03 | 大地绿源环保科技(北京)有限公司 | Bacillus methylotrophicus UTM401 and applications thereof |
| CN103172415A (en) * | 2011-12-26 | 2013-06-26 | 天津鹤岛农业科技有限公司 | Paenibacillus polymyxa microbial fertilizer produced through resource production of surplus sludge |
| CN103553739A (en) * | 2013-09-30 | 2014-02-05 | 眉县果业技术推广服务中心 | Production method for liquid organic fertilizer by using starch wastewater |
| CN104016733A (en) * | 2013-03-03 | 2014-09-03 | 湖北宜都科泰生物工程有限公司 | Multifunctional bio-organic fertilizer produced by utilizing resource recovery of yeast wastewater |
| CN105779323A (en) * | 2014-12-23 | 2016-07-20 | 中国科学院生态环境研究中心 | Bacillus subtilis capable of efficiently degrading quorum sensing signal molecules and production method of inoculant thereof |
| CN107034152A (en) * | 2016-10-11 | 2017-08-11 | 天津科技大学 | A kind of method that use sweet potato starch waste liquid prepares azotate pollution soil remediation microbial inoculum N4 |
| CN110643654A (en) * | 2019-10-17 | 2020-01-03 | 浙江大学 | Method for producing polysaccharide by fermenting starch wastewater |
| WO2023029213A1 (en) * | 2021-09-03 | 2023-03-09 | 河南科谱特医药科技研究院有限公司 | Fermentation process for preparing postbiotics by using wheat processing byproducts |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103172415A (en) * | 2011-12-26 | 2013-06-26 | 天津鹤岛农业科技有限公司 | Paenibacillus polymyxa microbial fertilizer produced through resource production of surplus sludge |
| CN103172415B (en) * | 2011-12-26 | 2015-05-13 | 天津鹤岛农业科技有限公司 | Paenibacillus polymyxa microbial fertilizer produced through resource production of surplus sludge |
| CN102703363A (en) * | 2012-05-14 | 2012-10-03 | 大地绿源环保科技(北京)有限公司 | Bacillus methylotrophicus UTM401 and applications thereof |
| CN104016733A (en) * | 2013-03-03 | 2014-09-03 | 湖北宜都科泰生物工程有限公司 | Multifunctional bio-organic fertilizer produced by utilizing resource recovery of yeast wastewater |
| CN103553739A (en) * | 2013-09-30 | 2014-02-05 | 眉县果业技术推广服务中心 | Production method for liquid organic fertilizer by using starch wastewater |
| CN103553739B (en) * | 2013-09-30 | 2015-06-10 | 眉县果业技术推广服务中心 | Production method for liquid organic fertilizer by using starch wastewater |
| CN105779323A (en) * | 2014-12-23 | 2016-07-20 | 中国科学院生态环境研究中心 | Bacillus subtilis capable of efficiently degrading quorum sensing signal molecules and production method of inoculant thereof |
| CN107034152A (en) * | 2016-10-11 | 2017-08-11 | 天津科技大学 | A kind of method that use sweet potato starch waste liquid prepares azotate pollution soil remediation microbial inoculum N4 |
| CN110643654A (en) * | 2019-10-17 | 2020-01-03 | 浙江大学 | Method for producing polysaccharide by fermenting starch wastewater |
| WO2023029213A1 (en) * | 2021-09-03 | 2023-03-09 | 河南科谱特医药科技研究院有限公司 | Fermentation process for preparing postbiotics by using wheat processing byproducts |
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Application publication date: 20111123 |