CN105439736A - Preparation method for solanaceous vegetable seedling medium - Google Patents

Preparation method for solanaceous vegetable seedling medium Download PDF

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Publication number
CN105439736A
CN105439736A CN201510984542.1A CN201510984542A CN105439736A CN 105439736 A CN105439736 A CN 105439736A CN 201510984542 A CN201510984542 A CN 201510984542A CN 105439736 A CN105439736 A CN 105439736A
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weight
nutrient solution
medium
preparation
bacterium chaff
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CN105439736B (en
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曾先富
潘绍坤
邓小晨
熊维全
李昕竺
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Chengdu Academy of Agriculture and Forestry Sciences
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Chengdu Academy of Agriculture and Forestry Sciences
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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
    • C05B1/02—Superphosphates
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F3/00—Fertilisers from human or animal excrements, e.g. manure
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Abstract

The invention provides a preparation method for a solanaceous vegetable seedling medium. The preparation method comprises the following steps: 1), preparing a zymogen; 2) preparing expanding propagation bacteria; 3) preparing a pig manure or chicken manure mushroom bran fermentation matrix; 4) preparing raw materials for the seedling medium; and 5) preparing a special seedling medium for solanaceous vegetables. According to the preparation method provided by the invention, two types of waste pollutants (livestock manure and mushroom bran) are simultaneously treated by applying an agricultural waste recycling technology and adopting a microbial conversion method; a preparation is performed according to a nutrient balance method, so that not only can cultivation wastes and edible mushroom wastes be handled, but also a high-quality efficient seedling medium can be developed, and the preparation method is conductive to promoting establishment of an ecological cycle chain and biological agriculture.

Description

For the preparation method of medium for culturing solanaceous fruit vegetable seedlings
technical field:
The invention belongs to agricultural wastes application technology as the second resource field, particularly be that one utilizes pig manure (chicken manure) and bacterium chaff mixed biologic to ferment the preparation method for medium for culturing solanaceous fruit vegetable seedlings developed.
background technology:
In recent years, along with the continuous expansion of modern intensive livestock and poultry cultivation and planting edible mushroom scale, the generation of feces of livestock and poultry and bacterium chaff is increasing, and according to measuring and calculating, China's feces of livestock and poultry generation in 2010 reaches 22.35 hundred million tons, year bacterium chaff generation reach 4,000 ten thousand tons.But, due to the restriction of recycling mode and transforming degree, cause unemployed feces of livestock and poultry and bacterium chaff to become the main source of rural environment pollution of area source, the benign development of serious harm related industries and new countryside construction.
Containing in feces of livestock and poultry and bacterium chaff and huge utilize potential benefit, is valuable Biological resources.Carrying out nutrient according to China's year livestock and poultry excretion fecaluria generation and amount to calculating, containing N about 1,957 ten thousand tons, is 78.9% of existing amount of application of nitrogen fertilizer; Containing P 2o 3about 3,630,000 tons is 57.4% of existing Phosphorus fertilizer usage; Containing K 2o about 1,341 ten thousand tons is 298% of existing Potassium Fertilizer.After testing, the bacterium chaff rich in nutrition content of the different planting materials such as cotton seed hulls, straw, corn cob, wood chip, organic content reaches more than 60%, is rich in the nutritive elements such as nitrogen, phosphorus, potassium, organic carbon, crude protein simultaneously.
China's grow seedlings of vegetable forward is changed and intensive future development on a fairly large scale.Meanwhile, more emphasizing today of food safety, realizing reducing using of agriculture chemical by nurturing staff, is the key of producing green ecological vegetables.Therefore, vegetable seedling substrate nutritive ingredient proportioning and nursery effect are the key links of vegetable seedling substrate research and development.At present, the raw material mainly peat composed of rotten mosses of seedling medium, but due to the excessive exploitation of the peat composed of rotten mosses, the environmental problem caused highlights, and the research of Peat substitution product becomes the focus of vegetable seedling substrate research.
Pass through literature search, in feces of livestock and poultry and bacterium chaff recycling, with bacterium chaff for raw material, simply ferment with perlite, vermiculite, slag, river sand, feces of livestock and poultry etc. and direct mixture, the report carrying out the vegetable seedling substrate researchs such as capsicum, tomato, cucumber is more.But bacterium Kang and pig manure (chicken manure) carry out biological fermentation and harmless treatment by special bacteria agent at high proportion, then for solanaceous vegetables nutritional characteristic in seedling stage, carry out nutritive equilibrium allotment, and the research preparing special medium for culturing solanaceous fruit vegetable seedlings has no report.
summary of the invention:
The object of the invention is to provide a kind of by using agricultural wastes application technology as the second resource, adopt microbial transformation mode, process two kinds of waste pollutants (feces of livestock and poultry and bacterium chaff) simultaneously, allocate according to nutritive equilibrium mode, both cultivation and waste edible fungus gurry had been solved, develop again high-quality and efficient seedling medium, be conducive to the preparation method for medium for culturing solanaceous fruit vegetable seedlings promoting that ecological circulation chain and biological husbantry are set up.
The object of the present invention is achieved like this:
The present invention is used for the preparation method of medium for culturing solanaceous fruit vegetable seedlings, comprises the following steps:
1) fermenting agent is prepared:
A: prepare bacterial classification: subtilis ( bacillussubtilisbacterium numbering CGMCC1.4600), Bacillus licheniformis ( bacilluslicheniformisbacterium numbering CICC10096), bacillus megaterium ( bacillusmegateriumbacterium numbering CGMCC1.217), Streptomyces jingyangensis ( streptomycesjingyangensisbacterium numbering ACCC40021), Fu Shi streptomyces verticillus ( streptomycesfradioverticillayusbacterium numbering CGMCC4.258), candida tropicalis ( candidatropicalisbacterium numbering ACCC20004), blown-ball Azotobacter ( azotobacterchroococcumbacterium numbering CGMCC1.2391), kluyveromyces marxianus bacterium [( kluyveromycesmarxianus), this bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC) on July 7th, 2014, deposit number is CGMCCNo.9427, and preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Institute of Microorganism, Academia Sinica];
B: prepare fermenting agent:
A) mixt bacteria nutrient solution is prepared:
The beef-protein medium production of hybrid seeds of subtilis, bacillus megaterium and vinelandii, producing method for seed is inoculated into respectively in the beef-protein medium of sterilizing by subtilis, bacillus megaterium and vinelandii, cultivate after 2 ~ 3 days for 30 ~ 35 DEG C and obtain bacillus subtilis bacteria culture fluid, bacillus megaterium nutrient solution, vinelandii nutrient solution, by bacillus subtilis bacteria culture fluid, bacillus megaterium nutrient solution, vinelandii nutrient solution by weight 1:1:1 mixing, become mixt bacteria nutrient solution;
In above-mentioned method, beef-protein medium compound method is as follows:
In peptone 10g, extractum carnis 6g, NaCl3g, water 1000g ratio batching, pH7.2 ~ 7.4 are regulated with 2NNaOH by after batching all the components heating for dissolving, then load in triangular flask, sterilizing 121 DEG C maintains 30 minutes and namely obtains beef-protein medium, for subsequent use after cooling;
B) preparation mixing actinomycetes nutrient solution:
Streptomyces jingyangensis and the Gao Shi culture medium culturing production of hybrid seeds of Fu Shi streptomyces verticillus, producing method for seed be Streptomyces jingyangensis and Fu Shi streptomyces verticillus to be inoculated into respectively in the Gao Shi substratum of sterilizing, 28 ~ 32 DEG C of shaking flasks concussion cultivations obtained Streptomyces jingyangensis nutrient solution, Fu Shi streptomyces verticillus nutrient solution respectively after 4 ~ 5 days, by Streptomyces jingyangensis nutrient solution, Fu Shi streptomyces verticillus nutrient solution by weight 1:1 mixing, become mixing actinomycetes nutrient solution;
In above-mentioned method, Gao Shi substratum compound method is as follows:
By Zulkovsky starch 10g, KNO 31g, K 2hPO 40.5g, MgSO 4﹒ 7H 2o0.5g, NaCl0.5g, FeSO 4﹒ 7H 2o0.01g, water 1000g ratio are prepared burden, and regulate pH7.2 ~ 7.4 after being dissolved by batching all the components with 2NNaOH, load in triangular flask, and sterilizing 121 DEG C maintains 30 minutes and namely obtains Gao Shi substratum, for subsequent use after cooling;
C) mixed yeast bacteria culture fluid is prepared:
Candida tropicalis and kluyveromyces marxianus bacterium potato sucrose medium cultivate the production of hybrid seeds, producing method for seed is inoculated into respectively in the potato sucrose medium of sterilizing by candida tropicalis and kluyveromyces marxianus bacterium, cultivate after 2 ~ 3 days for 30 ~ 33 DEG C and obtain candida tropicalis nutrient solution, kluyveromyces marxianus bacteria culture fluid respectively, by candida tropicalis nutrient solution, kluyveromyces marxianus nutrient solution by weight 1:1 mixing, become mixed yeast bacteria culture fluid;
In above-mentioned method, potato sucrose medium compound method is as follows:
Fresh potato is peeled, is cut into finger size fritter, takes potato block 200g, add water 1000g, low baking temperature boils 20 minutes, with gauze elimination solid residue, get supernatant liquor, the constant volume that adds water is to 1000g, add sucrose 20g again, after dissolving, load in triangular flask, sterilizing 121 DEG C maintains 30 minutes, namely obtained potato sucrose medium, for subsequent use after cooling;
D) fermenting agent is prepared:
Mixt bacteria nutrient solution, mixing actinomycetes nutrient solution and mixed yeast bacteria culture fluid are mixed by weight 1 ~ 1.5:0.5 ~ 1:0.5 ~ 1, then mixed bacteria liquid total amount mixed by weight 1:1 with wheat bran, 40 ~ 50 DEG C of dryings, obtain fermenting agent;
2) numerous microbial inoculum is expanded in preparation: by weight, fermenting agent: Semen Maydis powder: the ratio batching of water=0.05 ~ 0.2:15 ~ 25:80 ~ 100, load in plastic tank or stainless steel cask, tighten bung with plastic cloth, namely 25 ~ 30 DEG C of fermentations obtained the numerous microbial inoculum of expansion for the treatment of feces of livestock and poultry and bacterium chaff mixture after 1 week;
3) pig manure or chicken manure bacterium chaff fermentation base-material is prepared;
A: wood-decay fungi bacterium chaff bag is carried out broken bag, carry out grinding with organic resource millerator to be again separated, obtain wood-decay fungi bacterium chaff slag, water ratio 55 ~ 60% wood-decay fungi bacterium chaff is prepared burden with the numerous microbial inoculum of expansion mix by weight 1000g:0.1 ~ 0.3g ratio, 25 ~ 30 DEG C of heap fermentations 5 days, obtain zymophyte chaff;
B: mixed with the ratio of fresh pig dung (or freshly-slaughtered poultry excrement) by weight 0.4 ~ 0.6:0.6 ~ 0.4 by zymophyte chaff, mixture moisture control, 50 ~ 60%, carries out heap fermentation, coating film heat and moisture preserving;
C: after banking up 5-7 days at envrionment temperature 25 ~ 30 DEG C, turning 1 time, continues fermentation 10-14 days of banking up, and obtains pig manure (or chicken manure) bacterium chaff fermentation base-material;
4) preparation of seedling medium formulation:
A: pig manure or chicken manure bacterium chaff fermentation base-material pulverizer are pulverized, the sieve crossing diameter 5mm is for subsequent use;
B: add auxiliary material, by weight farmland soil with the sieve screening farmland soil conduct of diameter 5mm: the batching of pig manure or chicken manure bacterium chaff fermentation base-material 1:0.8 ~ 1.2, mixes, namely become seedling medium formulation;
5) the special seedling medium of solanaceous vegetables is prepared:
A: by weight, by seedling medium formulation: urea: calcium superphosphate=1000:3 ~ 6:2 ~ 4 batching is carried out Homogeneous phase mixing and obtained compound;
B: by weight, compound: wood-decay fungi bacterium chaff: farmland soil: the proportions of wood chip=2:4 ~ 6:3 ~ 1:3 ~ 1 becomes medium for culturing solanaceous fruit vegetable seedlings product.
When preparing fermenting agent in above-mentioned method, by mixt bacteria nutrient solution, mixing actinomycetes nutrient solution and mixed yeast bacteria culture fluid by weight 1:1:1 mixing, mixed bacteria liquid total amount mixed by weight 1:1 with wheat bran, 40 ~ 50 DEG C of dryings, obtain fermenting agent again.
When in above-mentioned method, numerous microbial inoculum is expanded in preparation, by weight, fermenting agent: Semen Maydis powder: the ratio batching of water=0.1:20:100, load in plastic tank or stainless steel cask, tighten bung with plastic cloth, namely 25 ~ 30 DEG C of fermentations obtain the numerous microbial inoculum of expansion for the treatment of feces of livestock and poultry and bacterium chaff mixture after 1 week.
Above-mentioned when preparing pig manure or chicken manure bacterium chaff fermentation base-material, by water ratio 55 ~ 60% wood-decay fungi bacterium chaff with expand numerous microbial inoculum by weight 1000:0.1 ratio prepare burden and mix, 25 ~ 30 DEG C of heap fermentations 3 ~ 5 days, obtain zymophyte chaff; Zymophyte chaff and fresh pig dung or the freshly-slaughtered poultry excrement ratio by weight 1:1 mixed, mixture moisture control, 50 ~ 60%, carries out heap fermentation, coating film heat and moisture preserving;
Above-mentioned prepares in seedling medium formulation, by weight, farmland soil: pig manure or chicken manure bacterium chaff fermentation base-material=1:1. batching.
Above-mentioned prepares in the special seedling medium of solanaceous vegetables, by weight, by seedling medium formulation: urea: calcium superphosphate=1000:4:3 batching is carried out Homogeneous phase mixing and obtained compound; By weight, compound: wood-decay fungi bacterium chaff: farmland soil: the proportions of wood chip=2:5:1:2 becomes medium for culturing solanaceous fruit vegetable seedlings product.
The solanaceous vegetables seedling medium adopting invention preparation method to produce can directly use, and nursery suggestion adopts hole plate seedling growth.
The present invention for raw material, adopts microbial fermentation technology with pig manure (chicken manure) and bacterium chaff, for solanaceous vegetables nutritional characteristic in seedling stage, according to nutritive equilibrium allotment, prepares the special seedling medium of solanaceous vegetables.The present invention, by researchs such as biotechnology, nutritive equilibrium technology, grow seedlings of vegetable technology, has opened up the new way of agricultural wastes resource utilization efficiency utilization, has had high economy, society, ecology synthesis benefit.The inventive method can promote the use of other association areas such as the cultivation of biological equalizing bed, biological organic fertilizer development, promotes the utilization of agricultural wastes resource, promotes that other new industry develops.
embodiment:
Embodiment 1:
The present embodiment 1, for the preparation method of medium for culturing solanaceous fruit vegetable seedlings, comprises the following steps:
1) fermenting agent is prepared:
A: prepare bacterial classification: subtilis, Bacillus licheniformis, bacillus megaterium, Streptomyces jingyangensis, Fu Shi streptomyces verticillus, candida tropicalis, blown-ball Azotobacter, kluyveromyces marxianus bacterium;
B: prepare fermenting agent:
A) mixt bacteria nutrient solution is prepared:
The beef-protein medium production of hybrid seeds of subtilis, bacillus megaterium and vinelandii, producing method for seed is inoculated into respectively in the beef-protein medium of sterilizing by subtilis, bacillus megaterium and vinelandii, cultivate after 3 days for 35 DEG C and obtain bacillus subtilis bacteria culture fluid, bacillus megaterium nutrient solution, vinelandii nutrient solution, by bacillus subtilis bacteria culture fluid, bacillus megaterium nutrient solution, vinelandii nutrient solution by weight 1:1:1 mixing, become mixt bacteria nutrient solution;
Above-mentioned beef-protein medium compound method is as follows:
In peptone 10g, extractum carnis 6g, NaCl3g, water 1000g ratio batching, pH7.3 is regulated with 2NNaOH by after batching all the components heating for dissolving, then load in triangular flask, sterilizing 121 DEG C maintains 30 minutes and namely obtains beef-protein medium, for subsequent use after cooling;
B) preparation mixing actinomycetes nutrient solution:
Streptomyces jingyangensis and the Gao Shi culture medium culturing production of hybrid seeds of Fu Shi streptomyces verticillus, producing method for seed is inoculated into respectively in the Gao Shi substratum of sterilizing by Streptomyces jingyangensis and Fu Shi streptomyces verticillus, 32 DEG C of shaking flask concussion cultivations obtain Streptomyces jingyangensis nutrient solution, Fu Shi streptomyces verticillus nutrient solution after 3 days respectively, by Streptomyces jingyangensis nutrient solution, Fu Shi streptomyces verticillus nutrient solution by weight 1:1 mixing, become mixing actinomycetes nutrient solution;
Above-mentioned Gao Shi substratum compound method is as follows:
By Zulkovsky starch 10g, KNO 31g, K 2hPO 40.5g, MgSO 4﹒ 7H 2o0.5g, NaCl0.5g, FeSO 4﹒ 7H 2o0.01g, water 1000g ratio are prepared burden, and regulate pH7.3 after being dissolved by batching all the components with 2NNaOH, load in triangular flask, and sterilizing 121 DEG C maintains 30 minutes and namely obtains Gao Shi substratum, for subsequent use after cooling;
C) mixed yeast bacteria culture fluid is prepared:
Candida tropicalis and kluyveromyces marxianus bacterium potato sucrose medium cultivate the production of hybrid seeds, producing method for seed is inoculated into respectively in the potato sucrose medium of sterilizing by candida tropicalis and kluyveromyces marxianus bacterium, cultivate after 3 days for 33 DEG C and obtain candida tropicalis nutrient solution, kluyveromyces marxianus bacteria culture fluid respectively, by candida tropicalis nutrient solution, kluyveromyces marxianus nutrient solution by weight 1:1 mixing, become mixed yeast bacteria culture fluid;
Above-mentioned potato sucrose medium compound method is as follows:
Fresh potato is peeled, is cut into finger size fritter, takes potato block 200g, add water 1000g, low baking temperature boils 20 minutes, with gauze elimination solid residue, get supernatant liquor, the constant volume that adds water is to 1000g, add sucrose 20g again, after dissolving, load in triangular flask, sterilizing 121 DEG C maintains 30 minutes, namely obtained potato sucrose medium, for subsequent use after cooling;
D) fermenting agent is prepared:
By mixt bacteria nutrient solution, mixing actinomycetes nutrient solution and mixed yeast bacteria culture fluid by weight 1:1:1 mixing, then mixed bacteria liquid total amount mixed by weight 1:1 with wheat bran, 45 DEG C of dryings, obtain fermenting agent;
2) numerous microbial inoculum is expanded in preparation: by weight fermenting agent: Semen Maydis powder: the ratio batching of water=0.1:20:100, load in plastic tank or stainless steel cask, tighten bung with plastic cloth, namely 27 DEG C of fermentations obtained the numerous microbial inoculum of expansion for the treatment of feces of livestock and poultry and bacterium chaff mixture after 1 week;
3) pig manure (or chicken manure) bacterium chaff fermentation base-material is prepared;
A: wood-decay fungi bacterium chaff bag is carried out broken bag, carry out grinding with organic resource millerator to be again separated, obtain wood-decay fungi bacterium chaff slag, by water ratio 60% wood-decay fungi bacterium chaff with expand numerous microbial inoculum by weight 1000:0.1 ratio prepare burden and mix, 28 DEG C of heap fermentations 4 days, obtain zymophyte chaff;
B: mixed with the ratio of fresh pig dung (or freshly-slaughtered poultry excrement) by weight 1:1 by zymophyte chaff, mixture moisture control, 60%, carries out heap fermentation, coating film heat and moisture preserving;
C: after banking up 6 days at envrionment temperature 28 DEG C, turning 1 time, continues fermentation 12 days of banking up, and obtains pig manure (or chicken manure) bacterium chaff fermentation base-material;
4) preparation of seedling medium formulation:
A: pulverized by pig manure (or chicken manure) bacterium chaff fermentation base-material pulverizer, the sieve crossing diameter 5mm is for subsequent use;
B: add auxiliary material, by weight farmland soil with the sieve screening farmland soil conduct of diameter 5mm: the batching of pig manure or chicken manure bacterium chaff fermentation base-material 1:1, mixes, namely become seedling medium formulation;
5) the special seedling medium of solanaceous vegetables is prepared:
A: by weight, by seedling medium formulation: urea: calcium superphosphate=1000:4:3 batching is carried out Homogeneous phase mixing and obtained compound;
B: by weight, compound: wood-decay fungi bacterium chaff: farmland soil: the proportions of wood chip=2:5:1:2 becomes medium for culturing solanaceous fruit vegetable seedlings product, carries out pepper seedling raising test, emerge neat, bud ratio reaches 95%.
embodiment 2:
The present embodiment 2, for the method for medium for culturing solanaceous fruit vegetable seedlings, comprises the following steps:
1) fermenting agent is prepared:
A: prepare bacterial classification: prepare bacterial classification by the method for embodiment 1;
B: prepare fermenting agent: prepare mixt bacteria nutrient solution, mixing actinomycetes nutrient solution, mixed yeast bacteria culture fluid by the method for embodiment 1, by mixt bacteria nutrient solution, mixing actinomycetes nutrient solution and mixed yeast bacteria culture fluid by weight 1:0.5:0.8 mixing, again mixed bacteria liquid total amount is mixed by weight 1:1 with wheat bran, 45 DEG C of dryings, obtain fermenting agent;
2) numerous microbial inoculum is expanded in preparation: by weight, fermenting agent: Semen Maydis powder: the ratio batching of water=0.05:15:80, load in plastic tank (or stainless steel cask), tighten bung with plastic cloth, namely 25 DEG C of fermentations obtained the numerous microbial inoculum of expansion for the treatment of feces of livestock and poultry and bacterium chaff mixture after 1 week;
3) pig manure (or chicken manure) bacterium chaff fermentation base-material is prepared;
A: wood-decay fungi bacterium chaff bag is carried out broken bag, carry out grinding with organic resource millerator to be again separated, obtain wood-decay fungi bacterium chaff slag, water ratio 55% wood-decay fungi bacterium chaff is mixed by weight 1000:0.1 with the numerous microbial inoculum of expansion, 25 DEG C of heap fermentations 4 days, obtain zymophyte chaff;
B: mixed with the ratio of fresh pig dung (or freshly-slaughtered poultry excrement) by weight 0.4:0.5 by zymophyte chaff, mixture moisture control, 50%, carries out heap fermentation, coating film heat and moisture preserving;
C: after banking up 5 days at envrionment temperature 25 DEG C, turning 1 time, continues fermentation 10 days of banking up, and obtains pig manure (or chicken manure) bacterium chaff fermentation base-material;
4) preparation of seedling medium formulation:
A: pulverized by pig manure (or chicken manure) bacterium chaff fermentation base-material pulverizer, the sieve crossing diameter 5mm is for subsequent use;
B: with the sieve screening farmland soil of diameter 5mm as adding auxiliary material, by weight, farmland soil: the ratio batching of pig manure (or chicken manure) bacterium chaff fermentation base-material 1:0.8, mixes, namely become seedling medium formulation;
5) the special seedling medium of solanaceous vegetables is prepared:
A: by weight, by seedling medium formulation: urea: calcium superphosphate=1000:4:2 carries out Homogeneous phase mixing.
B: by weight, compound: wood-decay fungi bacterium chaff: farmland soil: the proportions of wood chip=2:5:2:1 becomes medium for culturing solanaceous fruit vegetable seedlings product, carries out eggplant seedling raising test, emerge neat, bud ratio reaches 96%.
embodiment 3:
The present embodiment 3, for the preparation method of medium for culturing solanaceous fruit vegetable seedlings, comprises above step:
1) fermenting agent is prepared:
A: prepare bacterial classification: prepare bacterial classification by embodiment 1 method;
B: prepare fermenting agent:
Mixt bacteria nutrient solution, mixing actinomycetes nutrient solution, mixed yeast bacteria culture fluid is prepared by embodiment 1 method, by mixt bacteria nutrient solution, mixing actinomycetes nutrient solution and mixed yeast bacteria culture fluid by weight 1.2:0.8:0.8 mixing, again mixed bacteria liquid total amount is mixed by weight 1:1 with wheat bran, 45 DEG C of dryings, obtain fermenting agent;
2) numerous microbial inoculum is expanded in preparation: by weight, fermenting agent: Semen Maydis powder: the ratio batching of water=0.1:20:100, load in plastic tank or stainless steel cask, tighten bung with plastic cloth, namely 28 DEG C of fermentations obtained the numerous microbial inoculum of expansion for the treatment of feces of livestock and poultry and bacterium chaff mixture after 1 week;
3) pig manure (or chicken manure) bacterium chaff fermentation base-material is prepared;
A: wood-decay fungi bacterium chaff bag is carried out broken bag, carry out grinding with organic resource millerator to be again separated, obtain wood-decay fungi bacterium chaff slag, by water ratio 60% wood-decay fungi bacterium chaff with expand numerous microbial inoculum by weight 1000:0.2 ratio prepare burden and mix, 28 DEG C of heap fermentations 3 days, obtain zymophyte chaff;
B: mixed with the ratio of fresh pig dung (or freshly-slaughtered poultry excrement) by weight 0.5:0.5 by zymophyte chaff, mixture moisture control, 60%, carries out heap fermentation, coating film heat and moisture preserving;
C: after banking up 7 days at envrionment temperature 28 DEG C, turning 1 time, continues fermentation 12 days of banking up, and obtains pig manure (or chicken manure) bacterium chaff fermentation base-material;
4) preparation of seedling medium formulation:
A: pulverized by pig manure (or chicken manure) bacterium chaff fermentation base-material pulverizer, the sieve crossing diameter 5mm is for subsequent use;
B: with the sieve screening farmland soil of diameter 5mm as adding auxiliary material, by weight, farmland soil: the ratio batching of pig manure (or chicken manure) bacterium chaff fermentation base-material 1:1, mixes, namely become seedling medium formulation;
5) the special seedling medium of solanaceous vegetables is prepared:
A: by weight, by seedling medium formulation: urea: calcium superphosphate=1000:5:2 carries out Homogeneous phase mixing.
B: by weight, compound: wood-decay fungi bacterium chaff: farmland soil: the proportions of wood chip=2:5:1:2 becomes medium for culturing solanaceous fruit vegetable seedlings product, carries out pepper seedling raising test, emerge neat, bud ratio reaches 100%.
embodiment 4:
The present embodiment 4, for the preparation method of medium for culturing solanaceous fruit vegetable seedlings, comprises the following steps:
1) fermenting agent is prepared:
A: prepare bacterial classification: prepare bacterial classification by embodiment 1 method;
B: prepare fermenting agent:
Mixt bacteria nutrient solution, mixing actinomycetes nutrient solution, mixed yeast bacteria culture fluid is prepared by embodiment 1 method, by mixt bacteria nutrient solution, mixing actinomycetes nutrient solution and mixed yeast bacteria culture fluid by weight 1.5:1:0.5 mixing, again mixed bacteria liquid total amount is mixed by weight 1:1 with wheat bran, 40 DEG C of dryings, obtain fermenting agent;
2) numerous microbial inoculum is expanded in preparation: by weight, fermenting agent: Semen Maydis powder: the ratio batching of water=0.2:25:90, load in plastic tank or stainless steel cask, tighten bung with plastic cloth, namely 26 DEG C of fermentations obtained the numerous microbial inoculum of expansion for the treatment of feces of livestock and poultry and bacterium chaff mixture after 1 week;
3) pig manure (or chicken manure) bacterium chaff fermentation base-material is prepared;
A: wood-decay fungi bacterium chaff bag is carried out broken bag, carry out grinding with organic resource millerator to be again separated, obtain wood-decay fungi bacterium chaff slag, water ratio 55% wood-decay fungi bacterium chaff is mixed by weight 1000g:0.3g with the numerous microbial inoculum of expansion, 30 DEG C of heap fermentations 5 days, obtain zymophyte chaff;
B: mixed with the ratio of fresh pig dung (or freshly-slaughtered poultry excrement) by weight 0.6:0.5 by zymophyte chaff, mixture moisture control, 55%, carries out heap fermentation, coating film heat and moisture preserving;
C: after banking up 7 days at envrionment temperature 30 DEG C, turning 1 time, continues fermentation 14 days of banking up, and obtains pig manure (or chicken manure) bacterium chaff fermentation base-material;
4) preparation of seedling medium formulation:
A: pulverized by pig manure (or chicken manure) bacterium chaff fermentation base-material pulverizer, the sieve crossing diameter 5mm is for subsequent use;
B: with the sieve screening farmland soil of diameter 5mm as adding auxiliary material, by weight, farmland soil: the ratio batching of pig manure (or chicken manure) bacterium chaff fermentation base-material 1:1.2, mixes, namely become seedling medium formulation;
5) the special seedling medium of solanaceous vegetables is prepared:
A: by weight, by seedling medium formulation: urea: calcium superphosphate=1000:6:2 carries out Homogeneous phase mixing.
B: by weight, compound: wood-decay fungi bacterium chaff: farmland soil: the proportions of wood chip=2:4:2:2 becomes medium for culturing solanaceous fruit vegetable seedlings product, carries out tomato seedling test, emerge neat, bud ratio reaches 95%.
embodiment 5:
The present embodiment 5, for the preparation method of medium for culturing solanaceous fruit vegetable seedlings, comprises the following steps:
1) fermenting agent is prepared:
A: prepare bacterial classification: prepare bacterial classification by embodiment 1 method;
B: prepare fermenting agent:
Mixt bacteria nutrient solution, mixing actinomycetes nutrient solution, mixed yeast bacteria culture fluid is prepared by embodiment 1 method, by mixt bacteria nutrient solution, mixing actinomycetes nutrient solution and mixed yeast bacteria culture fluid by weight 1:1:1 mixing, again mixed bacteria liquid total amount is mixed by weight 1:1 with wheat bran, 50 DEG C of dryings, obtain fermenting agent;
2) numerous microbial inoculum is expanded in preparation: by weight, fermenting agent: Semen Maydis powder: the ratio batching of water=0.05:20:90, load in plastic tank or stainless steel cask, tighten bung with plastic cloth, namely 27 DEG C of fermentations obtained the numerous microbial inoculum of expansion for the treatment of feces of livestock and poultry and bacterium chaff mixture after 1 week;
3) pig manure (or chicken manure) bacterium chaff fermentation base-material is prepared;
A: wood-decay fungi bacterium chaff bag is carried out broken bag, carry out grinding with organic resource millerator to be again separated, obtain wood-decay fungi bacterium chaff slag, water ratio 55% wood-decay fungi bacterium chaff is mixed by weight 1000:0.1 with the numerous microbial inoculum of expansion, 26 DEG C of heap fermentations 4 days, obtain zymophyte chaff;
B: mixed with the ratio of fresh pig dung (or freshly-slaughtered poultry excrement) by weight 0.4:0.6 by zymophyte chaff, mixture moisture control, 50%, carries out heap fermentation, coating film heat and moisture preserving;
C: after banking up 7 days at envrionment temperature 26 DEG C, turning 1 time, continues fermentation 10 days of banking up, and obtains pig manure (or chicken manure) bacterium chaff fermentation base-material;
4) preparation of seedling medium formulation:
A: pulverized by pig manure (or chicken manure) bacterium chaff fermentation base-material pulverizer, the sieve crossing diameter 5mm is for subsequent use;
B: add auxiliary material, by weight farmland soil with the sieve screening farmland soil conduct of diameter 5mm: the ratio of pig manure (or chicken manure) bacterium chaff fermentation base-material 1:0.8 is prepared burden, and mixes, namely becomes seedling medium formulation;
5) the special seedling medium of solanaceous vegetables is prepared:
A: by weight, by seedling medium formulation: urea: calcium superphosphate=1000:4:2 carries out Homogeneous phase mixing.
B: by weight, compound: wood-decay fungi bacterium chaff: farmland soil: the proportions of wood chip=2:6:1:1 becomes medium for culturing solanaceous fruit vegetable seedlings product, carries out eggplant seedling raising test, emerge neat, bud ratio reaches 98%.
embodiment 6:
The present embodiment 6, for the preparation method of medium for culturing solanaceous fruit vegetable seedlings, comprises the following steps:
1) fermenting agent is prepared:
A: prepare bacterial classification: prepare bacterial classification by embodiment 1 method;
B: prepare fermenting agent:
Mixt bacteria nutrient solution, mixing actinomycetes nutrient solution, mixed yeast bacteria culture fluid is prepared by embodiment 1 method, by mixt bacteria nutrient solution, mixing actinomycetes nutrient solution and mixed yeast bacteria culture fluid by weight 1.2:0.5:1 mixing, again mixed bacteria liquid total amount is mixed by weight 1:1 with wheat bran, 50 DEG C of dryings, obtain fermenting agent;
2) numerous microbial inoculum is expanded in preparation: by weight, fermenting agent: Semen Maydis powder: the ratio batching of water=0.05:25:100, load in plastic tank (or stainless steel cask), tighten bung with plastic cloth, namely 30 DEG C of fermentations obtained the numerous microbial inoculum of expansion for the treatment of feces of livestock and poultry and bacterium chaff mixture after 1 week;
3) pig manure (or chicken manure) bacterium chaff fermentation base-material is prepared;
A: wood-decay fungi bacterium chaff bag is carried out broken bag, carry out grinding with organic resource millerator to be again separated, obtain wood-decay fungi bacterium chaff slag, water ratio 60% wood-decay fungi bacterium chaff is mixed by weight 1000:0.2 with the numerous microbial inoculum of expansion, 27 DEG C of heap fermentations 5 days, obtain zymophyte chaff;
B: mixed with the ratio of fresh pig dung (or freshly-slaughtered poultry excrement) by weight 0.5:0.6 by zymophyte chaff, mixture moisture control, 55%, carries out heap fermentation, coating film heat and moisture preserving;
C: after banking up 8 days at envrionment temperature 27 DEG C, turning 1 time, continues fermentation 12 days of banking up, and obtains pig manure (or chicken manure) bacterium chaff fermentation base-material;
4) preparation of seedling medium formulation:
A: pulverized by pig manure (or chicken manure) bacterium chaff fermentation base-material pulverizer, the sieve crossing diameter 5mm is for subsequent use;
B: with the sieve screening farmland soil of diameter 5mm as adding auxiliary material, by weight, farmland soil: the ratio batching of pig manure or chicken manure bacterium chaff fermentation base-material 1:1, mixes, namely become seedling medium formulation;
5) the special seedling medium of solanaceous vegetables is prepared:
A: by weight, by seedling medium formulation: urea: calcium superphosphate=1000:5:3 carries out Homogeneous phase mixing.
B: by weight, compound: wood-decay fungi bacterium chaff: farmland soil: the proportions of wood chip=2:4:3:1 becomes medium for culturing solanaceous fruit vegetable seedlings product, carries out pepper seedling raising test, emerge neat, bud ratio reaches 98%.
embodiment 7:
The present embodiment 7, for the preparation method of medium for culturing solanaceous fruit vegetable seedlings, comprises the following steps:
1) fermenting agent is prepared:
A: prepare bacterial classification: prepare bacterial classification by embodiment 1 method;
B: prepare fermenting agent:
Mixt bacteria nutrient solution, mixing actinomycetes nutrient solution, mixed yeast bacteria culture fluid is prepared by embodiment 1 method, by mixt bacteria nutrient solution, mixing actinomycetes nutrient solution and mixed yeast bacteria culture fluid by weight 1.5:0.5:1 mixing, again mixed bacteria liquid total amount is mixed by weight 1:1 with wheat bran, 40 DEG C of dryings, obtain fermenting agent;
2) numerous microbial inoculum is expanded in preparation: by weight fermenting agent: Semen Maydis powder: the ratio batching of water=0.1:15:80, load in plastic tank or stainless steel cask, tighten bung with plastic cloth, namely 28 DEG C of fermentations obtained the numerous microbial inoculum of expansion for the treatment of feces of livestock and poultry and bacterium chaff mixture after 1 week;
3) pig manure (or chicken manure) bacterium chaff fermentation base-material is prepared;
A: wood-decay fungi bacterium chaff bag is carried out broken bag, carry out grinding with organic resource millerator to be again separated, obtain wood-decay fungi bacterium chaff slag, water ratio 55% wood-decay fungi bacterium chaff is mixed by weight 1000:0.3 with the numerous microbial inoculum of expansion, 25 DEG C of heap fermentations 4 days, obtain zymophyte chaff;
B: mixed with the ratio of fresh pig dung (or freshly-slaughtered poultry excrement) by weight 0.6:0.4 by zymophyte chaff, mixture moisture control, 60%, carries out heap fermentation, coating film heat and moisture preserving;
C: after banking up 7 days at envrionment temperature 29 DEG C, turning 1 time, continues fermentation 10 days of banking up, and obtains pig manure (or chicken manure) bacterium chaff fermentation base-material;
4) preparation of seedling medium formulation:
A: pulverized by pig manure (or chicken manure) bacterium chaff fermentation base-material pulverizer, the sieve crossing diameter 5mm is for subsequent use;
B: add auxiliary material, by weight farmland soil with the sieve screening farmland soil conduct of diameter 5mm: the ratio of pig manure (or chicken manure) bacterium chaff fermentation base-material 1:1.2 is prepared burden, and mixes, namely becomes seedling medium formulation;
5) the special seedling medium of solanaceous vegetables is prepared:
A: by weight, by seedling medium formulation: urea: calcium superphosphate=1000:6:4 carries out Homogeneous phase mixing.
B: by weight, compound: wood-decay fungi bacterium chaff: farmland soil: the proportions of wood chip=2:4:1 becomes medium for culturing solanaceous fruit vegetable seedlings product, carries out tomato seedling test, emerge neat, bud ratio reaches 96%.
embodiment 8:
The present embodiment 8, for the preparation method of medium for culturing solanaceous fruit vegetable seedlings, comprises the following steps:
1) fermenting agent is prepared:
A: prepare bacterial classification: prepare bacterial classification by embodiment 1 method;
B: prepare fermenting agent:
Mixt bacteria nutrient solution, mixing actinomycetes nutrient solution, mixed yeast bacteria culture fluid is prepared by embodiment 1 method, by mixt bacteria nutrient solution, mixing actinomycetes nutrient solution and mixed yeast bacteria culture fluid by weight 1.2:1:0.5 mixing, again mixed bacteria liquid total amount is mixed by weight 1:1 with wheat bran, 45 DEG C of dryings, obtain fermenting agent;
2) numerous microbial inoculum is expanded in preparation: by weight, fermenting agent: Semen Maydis powder: the ratio batching of water=0.1:25:90, load in plastic tank (or stainless steel cask), tighten bung with plastic cloth, namely 26 DEG C of fermentations obtained the numerous microbial inoculum of expansion for the treatment of feces of livestock and poultry and bacterium chaff mixture after 1 week;
3) pig manure (or chicken manure) bacterium chaff fermentation base-material is prepared;
A: wood-decay fungi bacterium chaff bag is carried out broken bag, carry out grinding with organic resource millerator to be again separated, obtain wood-decay fungi bacterium chaff slag, water ratio 60% wood-decay fungi bacterium chaff is mixed by weight 1000:0.1 with the numerous microbial inoculum of expansion, 26 DEG C of heap fermentations 4 days, obtain zymophyte chaff;
B: mixed with the ratio of fresh pig dung (or freshly-slaughtered poultry excrement) by weight 0.5:0.5 by zymophyte chaff, mixture moisture control, 50%, carries out heap fermentation, coating film heat and moisture preserving;
C: after banking up 5 days at envrionment temperature 30 DEG C, turning 1 time, continues fermentation 14 days of banking up, and ferment base-material to obtain pig manure (or chicken manure bacterium chaff);
4) preparation of seedling medium formulation:
A: pulverized by pig manure (or chicken manure) bacterium chaff fermentation base-material pulverizer, the sieve crossing diameter 5mm is for subsequent use;
B: with the sieve screening farmland soil of diameter 5mm as adding auxiliary material, by weight, farmland soil: the ratio batching of pig manure (or chicken manure) bacterium chaff fermentation base-material 1:0.8, mixes, namely become seedling medium formulation;
5) the special seedling medium of solanaceous vegetables is prepared:
A: by weight, by seedling medium formulation: urea: calcium superphosphate=1000:5:2 carries out Homogeneous phase mixing.
B: by weight, compound: wood-decay fungi bacterium chaff: farmland soil: the proportions of wood chip=2:5:1:2 becomes medium for culturing solanaceous fruit vegetable seedlings product, carries out eggplant seedling raising test, emerge neat, bud ratio reaches 97%.
embodiment 9:
The present embodiment 9, for the preparation method of medium for culturing solanaceous fruit vegetable seedlings, comprises the following steps:
1) fermenting agent is prepared:
A: prepare bacterial classification: prepare bacterial classification by embodiment 1 method;
B: prepare fermenting agent:
Mixt bacteria nutrient solution, mixing actinomycetes nutrient solution, mixed yeast bacteria culture fluid is prepared by embodiment 1 method, by mixt bacteria nutrient solution, mixing actinomycetes nutrient solution and mixed yeast bacteria culture fluid by weight 1.2:0.8:1 mixing, again mixed bacteria liquid total amount is mixed by weight 1:1 with wheat bran, 45 DEG C of dryings, obtain fermenting agent;
2) numerous microbial inoculum is expanded in preparation: by weight fermenting agent: Semen Maydis powder: the ratio batching of water=0.2:15:80, load in plastic tank (or stainless steel cask), tighten bung with plastic cloth, namely 30 DEG C of fermentations obtained the numerous microbial inoculum of expansion for the treatment of feces of livestock and poultry and bacterium chaff mixture after 1 week;
3) pig manure (or chicken manure) bacterium chaff fermentation base-material is prepared;
A: wood-decay fungi bacterium chaff bag is carried out broken bag, carry out grinding with organic resource millerator to be again separated, obtain wood-decay fungi bacterium chaff slag, water ratio 60% wood-decay fungi bacterium chaff is mixed by weight 1000:0.2 with the numerous microbial inoculum of expansion, 27 DEG C of heap fermentations 4 days, obtain zymophyte chaff;
B: mixed with the ratio of fresh pig dung (or freshly-slaughtered poultry excrement) by weight 0.4:0.5 by zymophyte chaff, mixture moisture control, 55%, carries out heap fermentation, coating film heat and moisture preserving;
C: after banking up 8 days at envrionment temperature 29 DEG C, turning 1 time, continues fermentation 13 days of banking up, and obtains pig manure (or chicken manure) bacterium chaff fermentation base-material;
4) preparation of seedling medium formulation:
A: pulverized by pig manure (or chicken manure) bacterium chaff fermentation base-material pulverizer, the sieve crossing diameter 5mm is for subsequent use;
B: with the sieve screening farmland soil of diameter 5mm as adding auxiliary material, by weight, farmland soil: the ratio batching of pig manure (or chicken manure) bacterium chaff fermentation base-material 1:1, mixes, namely become seedling medium formulation;
5) the special seedling medium of solanaceous vegetables is prepared:
A: by weight, by seedling medium formulation: urea: calcium superphosphate=1000:4:3 carries out Homogeneous phase mixing.
B: by weight, compound: wood-decay fungi bacterium chaff: farmland soil: the proportions of wood chip=2:5:2:1 becomes medium for culturing solanaceous fruit vegetable seedlings product, carries out pepper seedling raising test, emerge neat, bud ratio reaches 97%.
embodiment 10:
The present embodiment 10, for the preparation method of medium for culturing solanaceous fruit vegetable seedlings, comprises the following steps:
1) fermenting agent is prepared:
A: prepare bacterial classification: prepare bacterial classification by embodiment 1 method;
B: prepare fermenting agent:
Mixt bacteria nutrient solution, mixing actinomycetes nutrient solution, mixed yeast bacteria culture fluid is prepared by embodiment 1 method, by mixt bacteria nutrient solution, mixing actinomycetes nutrient solution and mixed yeast bacteria culture fluid by weight 1:0.8:0.8 mixing, again mixed bacteria liquid total amount is mixed by weight 1:1 with wheat bran, 40 DEG C of dryings, obtain fermenting agent;
2) numerous microbial inoculum is expanded in preparation: by weight, fermenting agent: Semen Maydis powder: the ratio batching of water=0.2:20:90, load in plastic tank (or stainless steel cask), tighten bung with plastic cloth, namely 25 DEG C of fermentations obtained the numerous microbial inoculum of expansion for the treatment of feces of livestock and poultry and bacterium chaff mixture after 1 week;
3) pig manure (or chicken manure) bacterium chaff fermentation base-material is prepared;
A: wood-decay fungi bacterium chaff bag is carried out broken bag, carry out grinding with organic resource millerator to be again separated, obtain wood-decay fungi bacterium chaff slag, water ratio 55% wood-decay fungi bacterium chaff is mixed by weight 1000:0.3 with the numerous microbial inoculum of expansion, 28 DEG C of heap fermentations 3 days, obtain zymophyte chaff;
B: mixed with the ratio of fresh pig dung (or freshly-slaughtered poultry excrement) by weight 0.6:0.5 by zymophyte chaff, mixture moisture control, 60%, carries out heap fermentation, coating film heat and moisture preserving;
C: after banking up 6 days at envrionment temperature 28 DEG C, turning 1 time, continues fermentation 13 days of banking up, and obtains pig manure (or chicken manure) bacterium chaff fermentation base-material;
4) preparation of seedling medium formulation:
A: pulverized by pig manure (or chicken manure) bacterium chaff fermentation base-material pulverizer, the sieve crossing diameter 5mm is for subsequent use;
B: with the sieve screening farmland soil of diameter 5mm as adding auxiliary material, by weight, farmland soil: the ratio batching of pig manure (or chicken manure) bacterium chaff fermentation base-material 1:1.2, mixes, namely become seedling medium formulation;
5) the special seedling medium of solanaceous vegetables is prepared:
A: by weight, by seedling medium formulation: urea: calcium superphosphate=1000:3:2 carries out Homogeneous phase mixing.
B: by weight, compound: wood-decay fungi bacterium chaff: farmland soil: the proportions of wood chip=2:6:1:1 becomes medium for culturing solanaceous fruit vegetable seedlings product, carries out tomato seedling test, emerge neat, bud ratio reaches 96%.
embodiment 11:
The present embodiment 11, for the preparation method of medium for culturing solanaceous fruit vegetable seedlings, comprises the following steps:
1) fermenting agent is prepared:
A: prepare bacterial classification: prepare bacterial classification by embodiment 1 method;
B: prepare fermenting agent:
Mixt bacteria nutrient solution, mixing actinomycetes nutrient solution, mixed yeast bacteria culture fluid is prepared by embodiment 1 method, by mixt bacteria nutrient solution, mixing actinomycetes nutrient solution and mixed yeast bacteria culture fluid by weight 1.2:0.5:0.5 mixing, again mixed bacteria liquid total amount is mixed by weight 1:1 with wheat bran, 50 DEG C of dryings, obtain fermenting agent;
2) numerous microbial inoculum is expanded in preparation: by weight, fermenting agent: Semen Maydis powder: the ratio batching of water=0.1:15:100, load in plastic tank or stainless steel cask, tighten bung with plastic cloth, namely 28 DEG C of fermentations obtained the numerous microbial inoculum of expansion for the treatment of feces of livestock and poultry and bacterium chaff mixture after 1 week;
3) pig manure or chicken manure bacterium chaff fermentation base-material is prepared;
A: wood-decay fungi bacterium chaff bag is carried out broken bag, carry out grinding with organic resource millerator to be again separated, obtain wood-decay fungi bacterium chaff slag, water ratio 60% wood-decay fungi bacterium chaff is mixed by weight 1000:0.2 with the numerous microbial inoculum of expansion, 29 DEG C of heap fermentations 5 days, obtain zymophyte chaff;
B: mixed with the ratio of fresh pig dung (or freshly-slaughtered poultry excrement) by weight 0.4:0.6 by zymophyte chaff, mixture moisture control, 60%, carries out heap fermentation, coating film heat and moisture preserving;
C: after banking up 7 days at envrionment temperature 27 DEG C, turning 1 time, continues fermentation 11 days of banking up, and obtains pig manure (or chicken manure) bacterium chaff fermentation base-material;
4) preparation of seedling medium formulation:
A: pulverized by pig manure (or chicken manure) bacterium chaff fermentation base-material pulverizer, the sieve crossing diameter 5mm is for subsequent use;
B: add auxiliary material, by weight farmland soil with the sieve screening farmland soil conduct of diameter 5mm: the ratio of pig manure (or chicken manure) bacterium chaff fermentation base-material 1:1 is prepared burden, and mixes, namely becomes seedling medium formulation;
5) the special seedling medium of solanaceous vegetables is prepared:
A: by weight, by seedling medium formulation: urea: calcium superphosphate=1000:3:3 carries out Homogeneous phase mixing.
B: by weight, compound: wood-decay fungi bacterium chaff: farmland soil: the proportions of wood chip=2:5:3:1 becomes medium for culturing solanaceous fruit vegetable seedlings product, carries out eggplant seedling raising test, emerge neat, bud ratio reaches 96%.
embodiment 12:
The present embodiment 12, for the preparation method of medium for culturing solanaceous fruit vegetable seedlings, comprises the following steps:
1) fermenting agent is prepared:
A: prepare bacterial classification: prepare bacterial classification by embodiment 1 method.
B: prepare fermenting agent:
Mixt bacteria nutrient solution, mixing actinomycetes nutrient solution, mixed yeast bacteria culture fluid is prepared by embodiment 1 method, by mixt bacteria nutrient solution, mixing actinomycetes nutrient solution and mixed yeast bacteria culture fluid by weight 1.2:0.8:0.8 mixing, again mixed bacteria liquid total amount is mixed by weight 1:1 with wheat bran, 50 DEG C of dryings, obtain fermenting agent;
2) numerous microbial inoculum is expanded in preparation: by weight, fermenting agent: Semen Maydis powder: the ratio batching of water=0.1:20:100, load in plastic tank or stainless steel cask, tighten bung with plastic cloth, namely 28 DEG C of fermentations obtained the numerous microbial inoculum of expansion for the treatment of feces of livestock and poultry and bacterium chaff mixture after 1 week;
3) pig manure (or chicken manure) bacterium chaff fermentation base-material is prepared;
A: wood-decay fungi bacterium chaff bag is carried out broken bag, carry out grinding with organic resource millerator to be again separated, obtain wood-decay fungi bacterium chaff slag, water ratio 60% wood-decay fungi bacterium chaff is mixed by weight 1000:0.3 with the numerous microbial inoculum of expansion, 30 DEG C of heap fermentations 4 days, obtain zymophyte chaff;
B: mixed with the ratio of fresh pig dung (or freshly-slaughtered poultry excrement) by weight 0.5:0.5 by zymophyte chaff, mixture moisture control, 60%, carries out heap fermentation, coating film heat and moisture preserving;
C: after banking up 5 days at envrionment temperature 26 DEG C, turning 1 time, continues fermentation 14 days of banking up, and obtains pig manure (or chicken manure) bacterium chaff fermentation base-material;
4) preparation of seedling medium formulation:
A: pulverized by pig manure (or chicken manure) bacterium chaff fermentation base-material pulverizer, the sieve crossing diameter 5mm is for subsequent use;
B: with the sieve screening farmland soil of diameter 5mm as adding auxiliary material, by weight, farmland soil: the ratio batching of pig manure or chicken manure bacterium chaff fermentation base-material 1:1, mixes, namely become seedling medium formulation;
5) the special seedling medium of solanaceous vegetables is prepared:
A: by weight, by seedling medium formulation: urea: calcium superphosphate=1000:5:2 carries out Homogeneous phase mixing.
B: by weight, compound: wood-decay fungi bacterium chaff: farmland soil: the proportions of wood chip=2:5:1:2 becomes medium for culturing solanaceous fruit vegetable seedlings product.
6) nursery simultaneous test:
A: substrate material:
The special seedling medium product of solanaceous vegetables prepared by aforesaid method is test materials, and the import adopted with Jin Tian company of maximum nursery enterprise of Sichuan Province and domestic seedling medium are contrast; Vegetable variety select Rong mix eggplant No. 8 eggplants, fragrant peppery overlord capsicum and century powder tomato.
B: test site:
Yang Ma test base, Chongzhou City of Chengdu Academy of Agriculture and Forestry Science.
C: test method:
This experiment was carried out March in spring ~ April in 2015, first hoted water treatment of seeds before sowing, and recycling facility plastic greenhouse, adopts isolated ecological hole plate seedling growth mode to carry out nursery.Concrete operations are: first flattened by seedling bed, spread mulch film and garden mould is isolated, then spread the bacterium slag blanket that becomes thoroughly decomposed on mulch film, then put cave dish becoming thoroughly decomposed on bacterium slag blanket, dish selection 50 cave, cave, sow after finally accomplishing fluently hole with cave dish point digger, every bunch planting 1 seed, each kind 20 is coiled, repeat for three times, sow the special seedling medium blinding of rear solanaceous vegetables, spread mulch film after watering and build Small plastic shed heat and moisture preserving, after band seed shows money or valuables one carries unintentionally, open mulch film hardening.After planting 40 days statistics bud ratios and growing state.
D: test-results:
Test-results can be found out: the nursery effect of substrate products on eggplant, capsicum, 3 Kinds of Tomato solanaceous vegetables of this research preparation is all better than contrast, average bud ratio reaches 96.2%, 99.5%, 96.6% respectively, sprout effective, neatly, nutrition supply balance, leaf look normal, seedling growth is good, healthy and strong.
Table 1: seedling growth test result cartogram
The inventive method is by using agricultural wastes application technology as the second resource, adopt microbial transformation mode, process two kinds of waste pollutants (feces of livestock and poultry and bacterium chaff) simultaneously, allocate according to nutritive equilibrium mode, both cultivation and waste edible fungus gurry had been solved, develop again high-quality and efficient seedling medium, be conducive to promoting that ecological circulation chain and biological husbantry are set up.
Above-described embodiment is further described foregoing of the present invention, but this should be interpreted as that the scope of the above-mentioned theme of the present invention is only limitted to above-described embodiment.All technology realized based on foregoing all belong to scope of the present invention.

Claims (6)

1., for the preparation method of medium for culturing solanaceous fruit vegetable seedlings, comprise the following steps:
1) fermenting agent is prepared:
A: prepare bacterial classification: subtilis, Bacillus licheniformis, bacillus megaterium, Streptomyces jingyangensis, Fu Shi streptomyces verticillus, candida tropicalis, blown-ball Azotobacter, kluyveromyces marxianus bacterium;
B: prepare fermenting agent:
A) mixt bacteria nutrient solution is prepared:
The beef-protein medium production of hybrid seeds of subtilis, bacillus megaterium and vinelandii, producing method for seed is inoculated into respectively in the beef-protein medium of sterilizing by subtilis, bacillus megaterium and vinelandii, cultivate after 2-3 days for 30 ~ 35 DEG C and obtain bacillus subtilis bacteria culture fluid, bacillus megaterium nutrient solution, vinelandii nutrient solution, by bacillus subtilis bacteria culture fluid, bacillus megaterium nutrient solution, vinelandii nutrient solution by weight 1:1:1 mixing, become mixt bacteria nutrient solution;
Above-mentioned beef-protein medium compound method is as follows:
In peptone 10g, extractum carnis 6g, NaCl3g, water 1000g ratio batching, pH7.2 ~ 7.4 are regulated with 2NNaOH by after batching all the components heating for dissolving, then load in triangular flask, sterilizing 121 DEG C maintains 30 minutes and namely obtains beef-protein medium, for subsequent use after cooling;
B) preparation mixing actinomycetes nutrient solution:
Streptomyces jingyangensis and the Gao Shi culture medium culturing production of hybrid seeds of Fu Shi streptomyces verticillus, producing method for seed Streptomyces jingyangensis and Fu Shi streptomyces verticillus is inoculated into respectively in Gao Shi No. 1 substratum of sterilizing, 28 ~ 32 DEG C of shaking flask concussions are cultivated after 4-5 days and are obtained Streptomyces jingyangensis nutrient solution, Fu Shi streptomyces verticillus nutrient solution respectively, by Streptomyces jingyangensis nutrient solution, Fu Shi streptomyces verticillus nutrient solution by weight 1:1 mixing, become mixing actinomycetes nutrient solution;
Above-mentioned Gao Shi substratum compound method is as follows:
By Zulkovsky starch 10g, KNO 31g, K 2hPO 40.5g, MgSO 4﹒ 7H 2o0.5g, NaCl0.5g, FeSO 4﹒ 7H 2o0.01g, water 1000g ratio are prepared burden, and regulate pH7.2 ~ 7.4 after being dissolved by batching all the components with 2NNaOH, load in triangular flask, and sterilizing 121 DEG C maintains 30 minutes and namely obtains Gao Shi substratum, for subsequent use after cooling;
C) mixed yeast bacteria culture fluid is prepared:
Candida tropicalis and kluyveromyces marxianus bacterium potato sucrose medium cultivate the production of hybrid seeds, producing method for seed is inoculated into respectively in the potato sucrose medium of sterilizing by candida tropicalis and kluyveromyces marxianus bacterium, cultivate after 2-3 days for 30 ~ 33 DEG C and obtain candida tropicalis nutrient solution, kluyveromyces marxianus bacteria culture fluid respectively, by candida tropicalis nutrient solution, kluyveromyces marxianus nutrient solution by weight 1:1 mixing, become mixed yeast bacteria culture fluid;
Above-mentioned potato sucrose medium compound method is as follows:
Fresh potato is peeled, is cut into finger size fritter, takes potato block 200g, add water 1000g, low baking temperature boils 20 minutes, with gauze elimination solid residue, get supernatant liquor, the constant volume that adds water is to 1000g, add sucrose 20g again, after dissolving, load in triangular flask, sterilizing 121 DEG C maintains 30 minutes, namely obtained potato sucrose medium, for subsequent use after cooling;
D) fermenting agent is prepared:
Mixt bacteria nutrient solution, mixing actinomycetes nutrient solution and mixed yeast bacteria culture fluid are mixed by weight 1 ~ 1.5:0.5 ~ 1:0.5 ~ 1, then mixed bacteria liquid total amount mixed by weight 1:1 with wheat bran, 40 ~ 50 DEG C of dryings, obtain fermenting agent;
2) numerous microbial inoculum is expanded in preparation: by weight, fermenting agent: Semen Maydis powder: the ratio batching of water=0.05 ~ 0.2:15 ~ 25:80 ~ 100, load in plastic tank or stainless steel cask, tighten bung with plastic cloth, namely 25 ~ 30 DEG C of fermentations obtained the numerous microbial inoculum of expansion for the treatment of feces of livestock and poultry and bacterium chaff mixture after 1 week;
3) pig manure or chicken manure bacterium chaff fermentation base-material is prepared;
A: wood-decay fungi bacterium chaff bag is carried out broken bag, carry out grinding with organic resource millerator to be again separated, obtain wood-decay fungi bacterium chaff slag, water ratio 55 ~ 60% wood-decay fungi bacterium chaff is prepared burden with the numerous microbial inoculum of expansion mix by weight 1000:0.1 ~ 0.3 ratio, 25 ~ 30 DEG C of heap fermentations 3 ~ 5 days, obtain zymophyte chaff;
B: zymophyte chaff and fresh pig dung or the freshly-slaughtered poultry excrement ratio by weight 0.4 ~ 0.6:0.6 ~ 0.4 mixed, mixture moisture control, 50 ~ 60%, carries out heap fermentation, coating film heat and moisture preserving;
C: after banking up 5-7 days at envrionment temperature 25 ~ 30 DEG C, turning 1 time, continues fermentation 10-14 days of banking up, and obtains pig manure or chicken manure bacterium chaff fermentation base-material;
4) preparation of seedling medium formulation:
A: pig manure or chicken manure bacterium chaff fermentation base-material pulverizer are pulverized, the sieve crossing diameter 5mm is for subsequent use;
B: with the sieve screening farmland soil of diameter 5mm as adding auxiliary material, by weight, farmland soil: the batching of pig manure or chicken manure bacterium chaff fermentation base-material 1:0.8 ~ 1.2, mixes, namely become seedling medium formulation;
5) the special seedling medium of solanaceous vegetables is prepared:
A: by weight, by seedling medium formulation: urea: Homogeneous phase mixing is carried out in calcium superphosphate=1000:3 ~ 6:2 ~ 4;
B: by weight, compound: wood-decay fungi bacterium chaff: farmland soil: the proportions of wood chip=2:4 ~ 6:3 ~ 1:3 ~ 1 becomes medium for culturing solanaceous fruit vegetable seedlings product.
2. the preparation method for medium for culturing solanaceous fruit vegetable seedlings according to claim 1, when it is characterized in that preparing fermenting agent, by mixt bacteria nutrient solution, mixing actinomycetes nutrient solution and mixed yeast bacteria culture fluid by weight 1:1:1 mixing, again mixed bacteria liquid total amount is mixed by weight 1:1 with wheat bran, 40 ~ 50 DEG C of dryings, obtain fermenting agent.
3. the preparation method for medium for culturing solanaceous fruit vegetable seedlings according to claim 1 and 2, when it is characterized in that numerous microbial inoculum is expanded in preparation, by weight, fermenting agent: Semen Maydis powder: the ratio batching of water=0.1:20:100, load in plastic tank or stainless steel cask, tighten bung with plastic cloth, namely 25 ~ 30 DEG C of fermentations obtained the numerous microbial inoculum of expansion for the treatment of feces of livestock and poultry and bacterium chaff mixture after 1 week.
4. the preparation method for medium for culturing solanaceous fruit vegetable seedlings according to claim 1 and 2, when it is characterized in that preparing pig manure or chicken manure bacterium chaff fermentation base-material, water ratio 55 ~ 60% wood-decay fungi bacterium chaff is prepared burden with the numerous microbial inoculum of expansion mix by weight 1000:0.1 ratio, 25 ~ 30 DEG C of heap fermentations 3 ~ 5 days, obtain zymophyte chaff; Zymophyte chaff and fresh pig dung or the freshly-slaughtered poultry excrement ratio by weight 1:1 mixed, mixture moisture control, 50 ~ 60%, carries out heap fermentation, coating film heat and moisture preserving.
5. the preparation method for medium for culturing solanaceous fruit vegetable seedlings according to claim 1 and 2, is characterized in that preparing in seedling medium formulation, by weight, farmland soil: pig manure or chicken manure bacterium chaff fermentation base-material=1:1 batching.
6. the preparation method for medium for culturing solanaceous fruit vegetable seedlings according to claim 1 and 2, it is characterized in that preparing in the special seedling medium of solanaceous vegetables, by weight, by seedling medium formulation: urea: calcium superphosphate=1000:4:3 batching is carried out Homogeneous phase mixing and obtained compound; By weight, compound: wood-decay fungi bacterium chaff: farmland soil: the proportions of wood chip=2:5:1:2 becomes medium for culturing solanaceous fruit vegetable seedlings product.
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