CN103435383A - Special microbial fertilizer for seaside solonchak, and preparation method and application of microbial fertilizer - Google Patents
Special microbial fertilizer for seaside solonchak, and preparation method and application of microbial fertilizer Download PDFInfo
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- CN103435383A CN103435383A CN201310365414XA CN201310365414A CN103435383A CN 103435383 A CN103435383 A CN 103435383A CN 201310365414X A CN201310365414X A CN 201310365414XA CN 201310365414 A CN201310365414 A CN 201310365414A CN 103435383 A CN103435383 A CN 103435383A
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- saline
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention relates to the technical field of soil fertilizers, in particular to special microbial fertilizer for seaside solonchak, and a preparation method and an application of the microbial fertilizer. The microbial fertilizer comprises the following components in percentage by weight: 35-45% of sludge, 20-25% of cow dung, 10-15% of fowl manure, 15-25% of edible fungi cultivation wastes, 8-12% of arbuscular mycorrhiza funguses, 10-15% of salt marsh apophysis mold and 0.05-0.15% of pyroligneous liquor. The microbial fertilizer has the effects of prompting formation of seaside heavy solonchak soil aggregation, reducing the pH value and the salt content of the soil, improving the soil fertility, and prompting crops to absorb a great amount of essential elements and microelements, such as nitrogen, phosphorus and potassium, and the purposes of prompting the crops to grow and increasing the yield are achieved.
Description
Technical field
The invention belongs to the soil and fertilizer technical field, be specifically related to a kind of seaside saline-soil microbial fertilizer special and its preparation method and application, be a kind ofly can promote the seaside saline-soil soil granular to form, reduce soil pH value and saltiness, increase soil fertility, promote microbial fertilizer of the macroelement such as Crop nitrogen, phosphorus, potassium and trace element and its preparation method and application.
Background technology
Nearly 0.2 hundred million hectares of China's seaside saline-soil, 3,000,000 hectares of left and right of coastal saline soil area, be the important standby land resources of China.Growing and can utilize under the background that arable land sharply reduces at current China's population, the soil of beach beach is utilized as to be alleviated cultivated land demand pressure an important directions is provided.Because the supersalinity of seaside saline-soil causes available N and P, potassium, organic equal size low, the pH value is higher, and the characteristic severe inhibition crop seed germination rate that water-permeable and air permeable is poor, easily harden, make that the crop seedling surviving rate is low, growing way is poor, finally causes output to decline to a great extent.The utilization ratio that how to improve seaside saline-soil is to be badly in need of the major issue solved.
Summary of the invention
The object of the present invention is to provide a kind of seaside saline-soil microbial fertilizer special and its preparation method and application, with solve the seaside saline-soil Soil structure poor, harden serious, and the problem such as high, the organic famine of inorganic salt content, available state N-P-K content be low.
The cardinal principle of seaside saline-soil microbial fertilizer special function of the present invention is as follows:
Contain the nutritive ingredients such as mass efficient state nitrogen, phosphorus, potassium, organic matter in the contained mud of seaside saline-soil microbial fertilizer special of the present invention, cow dung and chicken manure, can effectively improve the solonchak fertility; Above-mentioned material and edible fungus culturing waste material can improve the salt soil structure on the one hand after fermentation, and good absorption and breeding environment can be provided for the microorganism that the later stage adds on the other hand; Contained sabkha apophysis mould (Apophysomyces spartina) separation, from the beach strongly salined soil, can also be secreted in a large number organic acids by Fast-propagation in hypersaline environment, and reduction solonchak pH value, dissolving insoluble phosphate supply plant to absorb; Contained bush mycorrhizal fungi (Arbuscular mycorrhizal fungus) can promote plant to absorb mineral nutrition (particularly phosphorus element nutrition), improving the salt stress-resistant ability of plant, and can secrete a kind of " super glue "---glomalin forms to accelerate soil aggregate; Contained wood vinegar can reduce soil pH value, Promoting plant growth, can also promote the increment of soil beneficial microorganism.
Seaside saline-soil microbial fertilizer special of the present invention comprises following component by weight percentage:
Mud 35～45%, cow dung 15～20%, chicken manure 10～15%, edible fungus culturing waste material 15～25%, bush mycorrhizal fungi 8～12%, sabkha apophysis mould 10～15%, wood vinegar 0.05～0.15%
The preparation method of seaside saline-soil microbial fertilizer special of the present invention comprises the following steps:
Step 1: 35～45% mud, 15～20% cow dungs, 10～15% chicken manures, 15～25% edible fungus culturing waste materials and 0.05～0.15% wood vinegar are mixed.
Step 2: the material mixed with the plastics film sealing, ferment 10～12 days.3rd, the turning of 6,9 days difference once.
Step 3: the material fermented is dried and pulverized.
Step 4: prepare sabkha apophysis mould.
Step 5: prepare bush mycorrhizal fungi.
Step 6: 8～12% bush mycorrhizal fungis and 10～15% sabkha apophysis moulds are fully sneaked into to the material crushed, and then 50～60 ℃ of oven dry get final product.
Described mud is discarded mud after brewage sewage is processed;
Described wood vinegar is resulting by plant materials (comprising root, stem, leaf, shell etc.) heating pyrolyze;
Described sabkha apophysis mould (Apophysomyces spartina) is from Yancheng wilderness area spartina alterniflora loisel's beach soil; be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on June 19th, 2013; preserving number is CGMCCNo.7717; No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, preservation address, Beijing institute of microbiology of the Chinese Academy of Sciences.
Described bush mycorrhizal fungi is Glomus mosseae (Glomus mosseae), from Inst. of Plant Nutrition &. Resource, Beijing City Academy of Agricultural &. Fo " Arbuscular Mycorrhizal Fungi In China germplasm resource bank (BGC) ", buys.
Described while preparing sabkha apophysis mould Martin's substratum used need to add NaCl2g
The present invention compared with the prior art, its significance progress is: under the prerequisite without inorganic nutriment such as extra interpolation nitrogen, phosphorus, potassium, this microbial fertilizer can significantly improve available state nutrient composition content in solonchak, reduces pH value and saltiness, significantly improves Soil structure; Improve the assimilated efficiency of crop to mineral nutrition, strengthen crop salt stress-resistant ability, reach the purpose of growth-promoting volume increase; And can make probiotics viable count in soil maintain for a long time higher level; Brought into play vital role in reclamation of saline soil by the sea, simultaneously the present invention also provides Processes For Effective Conversion for brew-house and livestock and poultry breeding industry waste.
Embodiment
Embodiment mono-: the preparation of seaside saline-soil microbial fertilizer special (A)
1. basic material preparation: at first 35% mud, 20% cow dung, 10% chicken manure, 15% edible fungus culturing waste material and 0.05% wood vinegar are mixed in whipping device.Then the material mixed with the plastics film sealing, ferment 10～12 days.3rd, the turning of 6,9 days difference once.Finally the material fermented is dried and pulverized.
2. the preparation of sabkha apophysis mould:
(1) slant strains activation: will be kept at-20 ℃ of bacterial classifications in refrigerator and be inoculated into improvement Martin substratum (K
2hPO
41g, MgSO
47H
2o0.5g, NaCl2g, peptone 5g, glucose 10g, agar powder 10g, 1/30000 rose-bengal aqueous solution 100mL, water 900mL, natural pH, 121 ℃ of moist heat sterilization 30min) on test tube slant, cultivate 4 days for 28 ℃.
(2) primary seed solution preparation: every test tube slant adds the sterilized water of 3mL, is deployed into bacterium liquid, pours (K in the liquid Martin substratum of 50mL into
2hPO
41g, MgSO
47H
2o0.5g, NaCl2g, peptone 5g, glucose 10g, 1/30000 rose-bengal aqueous solution 100mL, water 900mL, natural pH, 121 ℃ of moist heat sterilization 30min), shaking table 180rpm, cultivate 48h for 28 ℃.
(3) second order fermentation liquid preparation: by 5% inoculum size, primary seed solution is added to (formula is with 2) in liquid Martin substratum, liquid amount 150mL/250mL, shaking table 180rpm, encapsulate after 28 ℃ of cultivation 96h, obtains the mould microbial inoculum of liquid sabkha apophysis.
3. the preparation of bush mycorrhizal fungi:
(1) bush mycorrhizal fungi expands numerous: take yellow sand and vermiculite (volume ratio 1: 1) is culture medium, matrix is crossed to 20 mesh sieves and is carried out damp and hot tyndallization twice (121 ℃ of 1h, carry out sterilizing for the second time after taking out naturally cooling after sterilizing for the first time), the ratio that is 1: 100 according to weight ratio by the bush mycorrhizal fungi buied (contain bush mycorrhizal fungi spore, mycelia, infect the sandy soil of root segment) and culture medium mixes, and puts into cultivation tray and waters permeable standby; Take rye grass as host plant, by commercial ryegrass seed with 75% after alcohol-pickled 5 minutes with aseptic water washing for several times, then be sprinkled in cultivation tray and cover again the thick matrix of 1cm, then be placed on and cultivate indoor cultivation and get final product in 60 days.
(2) microbial inoculum is collected: after 60 days, the over-ground part of rye grass is all cut, root and all matrix suitably dry rear input pulverizer and pulverize and can obtain bush mycorrhizal fungi preparation.
4. microbial fertilizer preparation: 8% bush mycorrhizal fungi and 11.95% sabkha apophysis mould are fully sneaked into to the material crushed, and then 50～60 ℃ of oven dry get final product.
Embodiment bis-: the preparation of seaside saline-soil microbial fertilizer special (B)
1. basic material preparation: at first 40% mud, 15% cow dung, 10% chicken manure, 15% edible fungus culturing waste material and 0.1% wood vinegar are mixed in whipping device.Then the material mixed with the plastics film sealing, ferment 10～12 days.3rd, the turning of 6,9 days difference once.Finally the material fermented is dried and pulverized.
2. the preparation of sabkha apophysis mould:
(1) slant strains activation: will be kept at-20 ℃ of bacterial classifications in refrigerator and be inoculated into improvement Martin substratum (K
2hPO
41g, MgSO
47H
2o0.5g, NaCl2g, peptone 5g, glucose 10g, agar powder 10g, 1/30000 rose-bengal aqueous solution 100mL, water 900mL, natural pH, 121 ℃ of moist heat sterilization 30min) on test tube slant, cultivate 4 days for 28 ℃.
(2) primary seed solution preparation: every test tube slant adds the sterilized water of 3mL, is deployed into bacterium liquid, pours (K in the liquid Martin substratum of 50mL into
2hPO
41g, MgSO
47H
2o0.5g, NaCl2g, peptone 5g, glucose 10g, 1/30000 rose-bengal aqueous solution 100mL, water 900mL, natural pH, 121 ℃ of moist heat sterilization 30min), shaking table 180rpm, cultivate 48h for 28 ℃.
(3) second order fermentation liquid preparation: by 5% inoculum size, primary seed solution is added to (formula is with 2) in liquid Martin substratum, liquid amount 150mL/250mL, shaking table 180rpm, encapsulate after 28 ℃ of cultivation 96h, obtains the mould microbial inoculum of liquid sabkha apophysis.
3. the preparation of bush mycorrhizal fungi:
(1) bush mycorrhizal fungi expands numerous: take yellow sand and vermiculite (volume ratio 1: 1) is culture medium, matrix is crossed to 20 mesh sieves and is carried out damp and hot tyndallization twice (121 ℃ of 1h, carry out sterilizing for the second time after taking out naturally cooling after sterilizing for the first time), the ratio that is 1: 100 according to weight ratio by the bush mycorrhizal fungi buied (contain bush mycorrhizal fungi spore, mycelia, infect the sandy soil of root segment) and culture medium mixes, and puts into cultivation tray and waters permeable standby; Take rye grass as host plant, by commercial ryegrass seed with 75% after alcohol-pickled 5 minutes with aseptic water washing for several times, then be sprinkled in cultivation tray and cover again the thick matrix of 1cm, then be placed on and cultivate indoor cultivation and get final product in 60 days.
(2) microbial inoculum is collected: after 60 days, the over-ground part of rye grass is all cut, root and all matrix suitably dry rear input pulverizer and pulverize and can obtain bush mycorrhizal fungi preparation.
4. microbial fertilizer preparation: 9% bush mycorrhizal fungi and 10.9% sabkha apophysis mould are fully sneaked into to the material crushed, and then 50～60 ℃ of oven dry get final product.
Embodiment tri-: the application of seaside saline-soil microbial fertilizer special
Microbial fertilizer of the present invention can significantly improve the solonchak situation, promotes plant growth and improves output.Carried out controlled trial in May, 2012 to October in Qing Kou saltern, Lianyungang.This ground soil is clay loam, saltiness 13.1g/kg, organic 4.4g/Kg, pH9.1, rapid available phosphorus 7.3mg/Kg, available potassium 547.3mg/Kg, available nitrogen 48.6mg/Kg.Trial crops is kostelezkya virginica and castor-oil plant, and every kind of crop is established three kinds of processing: use the microbial fertilizer (A) of embodiment mono-preparation, the microbial fertilizer (B) of using embodiment bis-preparations, contrast (CK).Before sowing, fertilizer treatment is that 20g corresponding microorganism fertilizer is put in every cave, and any fertilizer is not executed in contrast.Routine Management in process of the test.The test of bear building-up bundle, gathered crop plant, seed, rhizosphere soil and measured each index October, and test-results is as follows:
Table 1: use the impact of microbial fertilizer on plant growth and output
Table 2: use the impact of microbial fertilizer on soil property
Result shows, uses that microbial fertilizer of the present invention can improve respectively kostelezkya virginica and more than castor-oil plant output 57.8% and 35.1%; Can effectively reduce soil pH and saltiness, the highlyest can improve 3 times of organic content, promote the formation of soil aggregate, can make microbes biomass carbon improve more than 4 times.
Claims (4)
1. a seaside saline-soil microbial fertilizer special consists of following component by weight percentage:
Mud 35～45%, cow dung 15～20%, chicken manure 10～15%, edible fungus culturing waste material 15～25%, bush mycorrhizal fungi 8～12%, sabkha apophysis mould 10～15%, wood vinegar 0.05～0.15%.
2. a kind of preparation method of seaside saline-soil microbial fertilizer special according to claim 1 is characterized in that consisting of following steps:
Step 1: 35～45% mud, 15～20% cow dungs, 10～15% chicken manures, 15～25% edible fungus culturing waste materials and 0.05～0.15% wood vinegar are mixed;
Step 2: the material mixed with the plastics film sealing, ferment 10～12 days.3rd, the turning of 6,9 days difference once;
Step 3: the material fermented is dried and pulverized;
Step 4: prepare sabkha apophysis mould;
Step 5: prepare bush mycorrhizal fungi;
Step 6: 8～12% bush mycorrhizal fungis and 10～15% sabkha apophysis moulds are fully sneaked into and crushed
Material, then 50～60 ℃ of oven dry are the seaside saline-soil microbial fertilizer special.
3. a kind of preparation method of seaside saline-soil microbial fertilizer special according to claim 2, is characterized in that described mud is discarded mud after brewage sewage is processed; Described wood vinegar is resulting by plant materials root, stem, leaf, shell heating pyrolyze.
4. the described seaside saline-soil microbial fertilizer special of claim 1 application in reclamation of saline soil by the sea.
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Cited By (6)
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| CN103771921A (en) * | 2014-02-10 | 2014-05-07 | 金陵科技学院 | Artificial grass carbon prepared from kosteletzkya virginica straws as well as preparation method and application thereof |
| CN104291974A (en) * | 2014-09-28 | 2015-01-21 | 湖北中化东方肥料有限公司 | Microbial fertilizer capable of enhancing salt tolerance of plants and preparation method of microbial fertilizer |
| CN105237254A (en) * | 2015-09-11 | 2016-01-13 | 安徽丰原马鞍山生物化学有限公司 | Biological fertilizer |
| CN111286518A (en) * | 2020-03-24 | 2020-06-16 | 中华全国供销合作总社南京野生植物综合利用研究所 | Preparation method of biostimulant capable of degrading pesticide residue and reducing heavy metal content |
| CN111349572A (en) * | 2020-03-24 | 2020-06-30 | 中华全国供销合作总社南京野生植物综合利用研究所 | Preparation method of fertilizer additive capable of reducing pesticide residues and heavy metal content |
| CN112876320A (en) * | 2021-02-03 | 2021-06-01 | 山西大学 | Active pyrosalt alkaline earth modifier and preparation method thereof |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103771921A (en) * | 2014-02-10 | 2014-05-07 | 金陵科技学院 | Artificial grass carbon prepared from kosteletzkya virginica straws as well as preparation method and application thereof |
| CN104291974A (en) * | 2014-09-28 | 2015-01-21 | 湖北中化东方肥料有限公司 | Microbial fertilizer capable of enhancing salt tolerance of plants and preparation method of microbial fertilizer |
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| CN111286518A (en) * | 2020-03-24 | 2020-06-16 | 中华全国供销合作总社南京野生植物综合利用研究所 | Preparation method of biostimulant capable of degrading pesticide residue and reducing heavy metal content |
| CN111349572A (en) * | 2020-03-24 | 2020-06-30 | 中华全国供销合作总社南京野生植物综合利用研究所 | Preparation method of fertilizer additive capable of reducing pesticide residues and heavy metal content |
| CN111349572B (en) * | 2020-03-24 | 2022-08-19 | 中华全国供销合作总社南京野生植物综合利用研究所 | Preparation method of fertilizer additive capable of reducing pesticide residues and heavy metal content |
| CN111286518B (en) * | 2020-03-24 | 2022-11-22 | 中华全国供销合作总社南京野生植物综合利用研究所 | Preparation method of biostimulant capable of degrading pesticide residue and reducing heavy metal content |
| CN112876320A (en) * | 2021-02-03 | 2021-06-01 | 山西大学 | Active pyrosalt alkaline earth modifier and preparation method thereof |
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Application publication date: 20131211 |