CN104016733B - Yeast wastewater resource produces multifunctional biological fertilizer - Google Patents
Yeast wastewater resource produces multifunctional biological fertilizer Download PDFInfo
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- CN104016733B CN104016733B CN201310065793.0A CN201310065793A CN104016733B CN 104016733 B CN104016733 B CN 104016733B CN 201310065793 A CN201310065793 A CN 201310065793A CN 104016733 B CN104016733 B CN 104016733B
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Abstract
The present invention relates to a kind of method that yeast wastewater recycling produces multifunctional biological fertilizer, belong to high-concentration waste water recycling and technical field of biological organic fertilizer。The present invention adopt bacterial strain be from soil separation screening out one plant height effect plant growth-promoting bacteria Bacillus licheniformis (<i>bacillus licheniformis</i>) B4 bacterial strain, this bacterial strain is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and deposit number is: CGMCC No. 6677。The present invention utilizes yeast wastewater as fermentation medium, large-scale culture Bacillus licheniformis, and preparation has controlling plant diseases, promotes the biological organic fertilizer of plant growing, this explained hereafter is with low cost, simple to operate, it is also possible to make yeast wastewater get utilization, reduce disposal of pollutants。The biological organic fertilizer that the present invention produces, for the production of vegetable, is possible not only to improve yield, and can improve the quality of tea place and vegetable, it is shown that wide application prospect。
Description
Technical field
The present invention relates to a kind of method that yeast wastewater recycling produces multifunctional biological fertilizer, this biological organic fertilizer has antagonism plant disease, promotes plant growing and improve the function of crop quality, belongs to high-concentration waste water recycling and technical field of biological organic fertilizer。
Background technology
Yeast is a kind of single celled eukaryotic microorganism, and it is the microorganism applying the earliest on history of human civilization。At present, yeast is widely used in the industries such as food, wine brewing, medicine, cosmetics, feedstuff。In recent years, along with developing rapidly of China's pharmacy industry and grocery trade, yeast industry also develops rapidly therewith, and domestic active dry yeast yield alreadys more than 200,000 tons/year。Mainly there are Angel, Ma Li, Dan Baoli, power, Mingguang City etc. difficult to understand in existing fairly large yeast production company of China。These manufacturing enterprises change from small to big, and technology & equipment level steadily improves, and profit increases considerably, and enterprise develops towards specialized, maximization, grouping of the world economy direction。China is mainly by the blackstrap (beet molasses and cane molasses) the growth carbon source as yeast, and makes nutritive salt with ammonium sulfate, sodium chloride, magnesium sulfate, ammonium phosphate etc. and produce yeast。The organic substance in blackstrap can not be made full use of due to yeast, the new Organic substance that remaining organic substance and yeast produce in growth metabolism process has been enter in waste water, produce substantial amounts of high concentration and be difficult to the yeast wastewater degraded, often produce 1 ton of dry yeast and produce the waste water of 60 ~ 130m3。Along with the fast development of yeast industry, the pollution problem of environment is become increasingly conspicuous by yeast wastewater, it has also become restrict the bottleneck of domestic yeast enterprise development。
Yeast wastewater arises primarily at Yeast fermentation process centrifugation and the high-concentration waste water of defecator discharge, is generally 20t/t yeast。COD at 30000～80000mg/L, total nitrogen 500～1500mg/L, sulfate 2000mg/L, pH4.0～7.5。Main Ingredients and Appearance is yeast protein, cellulose, colloidal substance, and the nutritional labeling in blackstrap that is not fully utilized, and such as residual sugar etc., except high content of organics, the caramelization compound in yeast wastewater makes yeast wastewater color relatively deep, for dark brown；Simultaneously all higher possibly together with the microbial metabolic products in sweat, inorganic salts, sulfate concentration in yeast wastewater, result in wastewater treatment qualified discharge extremely difficult, processing cost is high；If dealt with improperly, it is easy to cause serious environmental pollution。The technique that current scientific research personnel have developed multiple process yeast wastewater, to improving treatment effeciency, reduce processing cost, such as: a kind of method of yeast wastewater advanced treating disclosed in Chinese invention patent (application number: 200710053598.0), first through biochemical treatment, sequentially add inorganic flocculating agent and organic flocculant, finally by activated carbon decolorizing。A kind of method for treating yeast wastewater disclosed in Chinese invention patent (application number: 200910114686.6), first carries out coagulating sedimentation pretreatment by waste water；Pretreated waste water is sequentially carried out anaerobic treatment and Aerobic Process for Treatment again, finally adopts Fenton advanced oxidation processes and air supporting to carry out deep oxidation process。A primary yeast High-concentration organic wastewater treatment method disclosed in Chinese invention patent (application number: 200910212384.2), mainly by high-concentration yeast wastewater evaporation and concentration, it is thus achieved that concentration is the concentrated solution of 40%-80%, and evaporating concentration process energy resource consumption is bigger。The process system and method for a kind of high-concentration yeast wastewater disclosed in Chinese invention patent (application number: 201110457764.X), mainly through anaerobic biological reactor fermentation methane, reclaim the energy, but the Pollutants in Wastewater concentration after anaerobic fermentation is still significantly high, in addition it is also necessary to subsequent treatment。Visible, develop simple and easy to do and good in economic efficiency yeast wastewater application technology as the second resource and remain a very urgent task。
Bacillus licheniformis (Bacilluslicheniformis) widely distributed in nature, it is a kind of important biocontrol microorganisms, it suppresses plant pathogenetic bacteria, fungus, virus, nematicide by producing antibiotic, antibacterial protein or polypeptide isoreactivity material, thus playing the effect of Biological control；It is also the plant rhizosphere growth-promoting antibacterial that a class is important simultaneously, shows the effect that various plants promotes growth。Such as, a bacillus licheniformis B-0A12 bacterial strain disclosed in Chinese invention patent (application number: 200310115535.5), deposit number is CGMCCNo.1035, for preventing and treating soybean cyst nematode and the microbial root rot of reaping hook, the culture medium used in this strain fermentation incubation is: soluble starch 1-2%, soybean cake powder 1-2%, Carnis Bovis seu Bubali cream 0.5-1.0%, glucose 0.1-0.5%, also has a small amount of inorganic salt, and during fermentation ends, bacterial content is 7 × 108Individual/milliliter。A plants endogenetic Bacillus licheniformis R21 bacterial strain disclosed in Chinese invention patent (application number: 200810026137.9), deposit number is CCTCCM207113, there is wide spectrum antagonistic plant pathogenic fungi ability, dissolving P capacity and promotion plant growing ability, its fermentation have employed LB culture medium, and disclose its preventing and treating Herba Apii graveolentis verticillium wilt, banana blight, and promote the application of Herba Apii graveolentis growth aspect。Bacillus licheniformis 201 bacterial strain disclosed in Chinese invention patent (application number: 200810183072.9), deposit number is CCTCCNo.M207092, the multiple pathogenic fungi such as banana blight, watermelon blight, tomato wilt, banana anthracnose, wheat scab are had antagonism by this bacterial strain, and the cultivation of this bacterial strain adopts beef-protein medium。Bacillus licheniformis 202 bacterial strain disclosed in Chinese invention patent (application number: 200810183075.2), deposit number is CCTCCNo.M207093, it also has the biological and ecological methods to prevent plant disease, pests, and erosion function similar with 201 bacterial strains。
The Bacillus licheniformis also ratio of application industrially is wide。Such as, a bacillus licheniformis U1-3 bacterial strain disclosed in Chinese invention patent (application number: 200810227858.6), deposit number is CGMCCNo.2437, this bacterial strain can be used to the displacement of reservoir oil in petroleum production engineering field, improving oil recovery, its culture medium have employed liquid paraffin, yeast powder and plurality of inorganic salt。A bacillus licheniformis WX-02 bacterial strain disclosed in Chinese invention patent (application number: 200810055068.4), deposit number is CCTCCNo.M208065, for producing Polyurethane-epoxy resin, its culture medium have employed sodium glutamate, carbon source (starch or the one in starch hydrolyzate, glucose, fructose, maltose, sucrose), inorganic nitrogen-sourced and other plurality of inorganic salt。A bacillus licheniformis HM-3 bacterial strain disclosed in Chinese invention patent (application number: 200810026812.8), deposit number is CGMCCNo.1970, is used for producing alkaline protease, and culture medium have employed yeast extract, peptone and municipal sludge。A bacillus licheniformis B-05571 bacterial strain disclosed in Chinese invention patent (application number: 200610053548.8), deposit number is CCTCCNo.M206082, for converting glycerol into dihydroxy acetone, culture medium, with glycerol for primary carbon source, also has yeast powder, peptone, Carnis Bovis seu Bubali cream, plurality of inorganic salt。
In sum, the bacterial strain of the Bacillus licheniformis with antagonism plant disease is more, and its cultural method is also different, but, have no and adopt yeast wastewater cultivate Bacillus licheniformis and use it for the report producing biological organic fertilizer。
Summary of the invention
It is an object of the invention to provide and a kind of can produce efficient antagonistic phytopathogen, promote plant growing and improve the method for Bacillus licheniformis biological organic fertilizer of crop quality by recycling yeast wastewater。
Bacterial strain used in the present invention be by inventor from soil separation screening out one plant height effect promote plant growing, antagonistic phytopathogen, raising crop quality Bacillus licheniformis (Bacilluslicheniformis) B4 bacterial strain, this bacterial strain is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on October 15th, 2012, and deposit number is: CGMCCNo.6677, depositary institution address: Datun Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode: 100101。
The production method of multifunctional biological fertilizer of the present invention is as follows:
1, yeast wastewater pretreatment: regulate the pH to 7.0～8.0 of yeast wastewater with the plant ash rich in potassium carbonate of biomass thermal power plant, in 110～121 DEG C of sterilizings 10～30 minutes。
2, prepared by liquid seeds: inoculate the Bacillus licheniformis of slant preservation in the triangular flask of the yeast wastewater good equipped with pretreatment, in 25～37 DEG C, and 120～200 revs/min of shaken cultivation 24～36 hours, obtain liquid seeds。
3, liquid fermentation: inoculating volume ratio in the yeast wastewater fermentation tank good equipped with pretreatment is the liquid seeds of 2%～15%, in 25～37 DEG C, logical filtrated air is cultivated 15～36 hours；Cultured fermentation liquor count plate, viable count reaches 9 × 108More than CFU/ milliliter, gained fermentation liquid can use after diluting as liquid bacterial agent。
4, the preparation of biological organic fertilizer: with the fertilizer that fully becomes thoroughly decomposed for carrier, according to fermentation liquid: fertilizer is the weight ratio of 1: 3～1: 10, fermentation liquid is added in fertilizer, it is sufficiently mixed uniformly, aeration-drying, to water content less than 30%, namely obtains Bacillus licheniformis biological organic fertilizer, packaging, leave shady and cool dry place in, standby。
The using method of Bacillus licheniformis multifunctional biological fertilizer of the present invention is as follows:
1, fermentation liquid irrigation water being diluted to viable count is 2 × 106CFU/ milliliter～5 × 107CFU/ milliliter, mix homogeneously, apply in the soil near crop root according to the consumption ditch of every mu of 1～5 cubic metre of diluent；
2, in Bacillus licheniformis biological organic fertilizer being manured into soil as base manure according to the consumption of 0.5～3 ton every mu, or in crop growth period, topdress and use。
Detailed description of the invention:
Can effectively be prevented and treated multiple soilborne fungal pathogens by Bacillus licheniformis biocontrol agent provided by the invention, and can promote that crop is to the absorption of nutrient and utilization, moreover it is possible to a certain amount of potash fertilizer is provided；What the agricultural microbial agent that technical scheme provided by the invention produces was applied to that agricultural production can reduce chemical fertilizer and pesticide makes consumption, thus improving farm environment quality；And production cost is very low, make high-concentration waste water get utilization, reduce pollutant emission。Detailed description of the invention is as follows:
Embodiment 1: yeast wastewater produces Bacillus licheniformis biological organic fertilizer
Yeast wastewater pretreatment: regulate the pH to about 7.5 of waste water with the plant ash rich in potassium carbonate of biomass thermal power plant, in 115 DEG C of sterilizings 15 minutes。
Prepared by liquid seeds: prepared by liquid seeds: inoculate the Bacillus licheniformis of slant preservation in the triangular flask of the yeast wastewater good equipped with pretreatment, in 35 DEG C, and 150 revs/min of shaken cultivation 24 hours, obtain liquid seeds。
Liquid fermentation: inoculating volume ratio in the yeast wastewater fermentation tank good equipped with pretreatment is the liquid seeds of 5%, in 35 DEG C, logical filtrated air is cultivated 20 hours；Cultured fermentation liquor count plate, viable count reaches 9 × 108More than CFU/ milliliter, gained fermentation liquid can use after diluting as liquid bacterial agent。
The preparation of biological organic fertilizer: with the fertilizer that fully becomes thoroughly decomposed for carrier, according to fermentation liquid: fertilizer is the weight ratio of 1: 5, fermentation liquid is added in fertilizer, it is sufficiently mixed uniformly, aeration-drying, to water content less than 30%, namely obtains Bacillus licheniformis biological organic fertilizer, packaging, leave shady and cool dry place in, standby。
Embodiment 2: Bacillus licheniformis biological organic fertilizer is used for preventing Fructus Cucumidis sativi damping off
Fructus Cucumidis sativi damping off is the disease caused by fungal infection。Gather the soil planted for many years in the warmhouse booth of Fructus Cucumidis sativi, inoculation scraping and printing unit (Pythiumaphanidermatum), mix homogeneously, load in flowerpot, 2 groups of flowerpots are set, often organize 10 basins, as, in the greenhouses of 15 DEG C～20 DEG C, keeping higher levels of humidity and relatively low intensity of illumination。The 1st group of direct sowing cucumber seed of flowerpot, 15, every basin, as comparison。2nd multicast kind cucumber seeds forward direction soil admixes the Bacillus licheniformis biocontrol agent that weight ratio is 0.3%, then every basin 15 seeds of sowing。Statistics sum after coming up, after seedling grows 1 true leaf, statistics lodging Seedling sum before 2 true leaves。It is shown that comparison seedling lodging rate is more than 65%, and adding the experimental group of microbial inoculum, seedling lodging rate is lower than 10%。
Embodiment 3: Bacillus licheniformis biological organic fertilizer is for improving the yield of vegetable and improving the quality of vegetable
Adopt the experiment of green house of vegetables community, 8 identical hillslope processes of condition, each community 60m are set2, 2 communities are 1 group of parallel test, are divided into into 4 groups, plant intensive shadow on lung, and at intensive shadow on lung Seedling Stage, do not apply fertilizer and compare in the 1st group of 2 communities；2nd group of 2 communities respectively use 1 × 107The Bacillus licheniformis biological organic fertilizer of CFU/ gram 100 kilograms, as base manure, respectively imposes one time 1 × 10 at Brassica campestris L complete germination after 3 weeks7The diluent of the lichen bacillus ferments liquid of CFU/ milliliter；Carbamide and is respectively used once after 3 weeks according to the dose of 15 kgs/acre after Brassica campestris L complete germination in 3rd group of 2 communities；And respectively use carbamide once after 3 weeks according to the dose of 15 kgs/acre after Brassica campestris L complete germination, and use Bacillus licheniformis biological organic fertilizer according to the 2nd prescription formula for 4th group 2, irrigation that each community is identical and way to manage。5 weeks " Invest, Then Investigate " yield and qualities of growth of rape。Individually use microbial manure and individually to use the yield of rape of carbamide basically identical, all than the check plot volume increase about 50% do not applied fertilizer；The ratio check plot volume increase about 80% that two kinds of fertilizer is all used；In Brassica campestris L, NO3-N and NO2-N content is not to apply fertilizer and individually to use the minimum of microbial bacterial agent, it is less than the 1/3 of national standard Limited Doses, use again limit the quantity also below national standard the 1/2 of microbial bacterial agent after using carbamide, and individually use NO3-N and NO2-N content in the Brassica campestris L of carbamide and all exceed standard seriously。Show the existing good effect of increasing production of this microbial bacterial agent, the quality of Brassica campestris L can be significantly improved again。
Claims (2)
1. the method utilizing yeast wastewater to produce Bacillus licheniformis biological organic fertilizer, comprises the steps:
(1) yeast wastewater pretreatment: regulate the pH to 7.0～8.0 of yeast wastewater with the plant ash rich in potassium carbonate of biomass thermal power plant, in 110～121 DEG C of sterilizings 10～30 minutes；
(2) prepared by liquid seeds: inoculate the Bacillus licheniformis of slant preservation in the triangular flask of the yeast wastewater good equipped with pretreatment, in 25～37 DEG C, and 120～200 revs/min of shaken cultivation 24～36 hours, obtain liquid seeds；
(3) liquid fermentation: inoculating volume ratio in the yeast wastewater fermentation tank good equipped with pretreatment is the liquid seeds of 2%～15%, in 25～37 DEG C, logical filtrated air is cultivated 15～36 hours；Cultured fermentation liquor count plate, viable count reaches 9 × 108More than CFU/ milliliter, gained fermentation liquid can use after diluting as liquid bacterial agent；
(4) preparation of biological organic fertilizer: with the fertilizer that fully becomes thoroughly decomposed for carrier, according to fermentation liquid: fertilizer is the weight ratio of 1: 3～1: 10, fermentation liquid is added in fertilizer, it is sufficiently mixed uniformly, aeration-drying, to water content less than 30%, namely obtains Bacillus licheniformis biological organic fertilizer, packaging, leave shady and cool dry place in, standby；
Described Bacillus licheniformis is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and deposit number is: CGMCCNo.6677。
2. described in claim 1, utilize yeast wastewater to produce the using method of Bacillus licheniformis biological organic fertilizer that the method for Bacillus licheniformis biological organic fertilizer prepares, including:
(1) fermentation liquid irrigation water being diluted to viable count is 2 × 106CFU/ milliliter～5 × 107CFU/ milliliter, mix homogeneously, apply in the soil near crop root according to the consumption ditch of every mu of 1～3 cubic metre of diluent；
(2) in Bacillus licheniformis biological organic fertilizer being manured into soil as base manure according to the consumption of 0.5～3 ton every mu, or in crop growth period, topdress and use。
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| UY36334A (en) * | 2014-12-29 | 2017-04-28 | Fmc Corp | COMPOSITIONS OF BACILLUS LICHENIFORMIS RT1184 AND USES METHODS TO BENEFIT THE GROWTH OF PLANTS |
| CN105132311B (en) * | 2015-08-10 | 2019-01-25 | 济南益邦生物科技有限公司 | Utilize the method for glutathione waste liquid production functional microorganism |
| CN105296561A (en) * | 2015-10-30 | 2016-02-03 | 梁山菱花生物科技有限公司 | Glutamic acid fermentation medium and preparation method thereof |
| CN108046873A (en) * | 2017-12-25 | 2018-05-18 | 中山市小榄企业服务有限公司 | A kind of yeast wastewater recycling produces multifunctional biological fertilizer |
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| JPH10114589A (en) * | 1996-10-09 | 1998-05-06 | Yoshimasa Hayashibara | Production of fertilizer from sea food waste |
| CN101734827B (en) * | 2009-12-29 | 2011-07-27 | 广西博世科环保科技股份有限公司 | Method for treating yeast wastewater |
| KR101212047B1 (en) * | 2009-12-31 | 2012-12-13 | 대한민국 | Bacterial strain Lactobacillus parakefiri NAAS-1 promoting plant growth |
| CN102249754A (en) * | 2010-05-17 | 2011-11-23 | 中国科学院生态环境研究中心 | Paenibacillus polymyxa microbial fertilizer produced through sweet potato starch waste water recovery |
| CN102093126A (en) * | 2010-12-03 | 2011-06-15 | 张新雄 | Method for producing bio-fertilizer from waste organic matters |
| CN102079675B (en) * | 2010-12-03 | 2013-07-17 | 董彬 | Magnetic biofertilizer and preparation method |
| CN102618527A (en) * | 2011-01-27 | 2012-08-01 | 中国科学院生态环境研究中心 | Method for recycling production of paenibacillus polymyxa microbial agent from citric acid wastewater |
| CN103820349B (en) * | 2012-11-16 | 2016-08-03 | 中国科学院生态环境研究中心 | One bacillus licheniformis and the production method of microbial inoculum thereof and purposes |
| CN102936166B (en) * | 2012-11-29 | 2015-01-07 | 南宁市英德肥业有限责任公司 | Water-soluble organic/inorganic compound fertilizer prepared by molasses fermentation liquor and preparation method of fertilizer |
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