CN101204159A - Biological control agents for plant diseases extracting from high concentration organic wastewater - Google Patents
Biological control agents for plant diseases extracting from high concentration organic wastewater Download PDFInfo
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- CN101204159A CN101204159A CNA2006101655951A CN200610165595A CN101204159A CN 101204159 A CN101204159 A CN 101204159A CN A2006101655951 A CNA2006101655951 A CN A2006101655951A CN 200610165595 A CN200610165595 A CN 200610165595A CN 101204159 A CN101204159 A CN 101204159A
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Abstract
The invention relates to a method for producing an anti-trichoderma agent by using organic wastewater with high concentration, belonging to the technical fields of biological pesticide and environmental pollution treatment. In the invention, the organic wastewater with the high concentration produced by fermentation industry and food-processing industry is taken as the main production material and a combined fermentation method in liquid state and solid sate is adopted to produce the anti-trichoderma agent. The production process includes: (1) the wastewater with balanced nutritional components is taken as a medium to carry out seed culture; (2) the organic wastewater with a high concentration is taken as a main medium to carry out liquid culture; (3) the wastewater through liquid culture and waste biomass are mixed to carry out solid-state fermentation;(4) after a drying treatment, fermentation products are added with a stabilizer and a promoter, and then crushed and packaged. The invention can also be directly to farmland soil, prevent and treat soil borne diseases, improve the soil and increase soil fertility. The anti-trichoderma agent produced by the process can effectively prevent and treat mycoses of a plurality of plants and has a low production cost; the agent can also be used for reducing wastewater discharge and recycling resources.
Description
Technical field
The invention belongs to biopesticide and environmental pollution treatment technology field.
Technical background
Fermentation industry, food-processing industry can produce a large amount of high concentrated organic wastewaters in process of production, as: the high concentrated organic wastewater amount that the glutamate production process is produced is big and difficult, and the potato process can produce the waste water of a large amount of high-content of starch.If make these high concentrated organic wastewater qualified discharges, disposal cost is high.And the large amount of organic in these waste water can be used as the nutriment of growth of microorganism, and discharging also is a kind of waste.
(Trichoderma sp.) is distributed more widely at occurring in nature for Trichoderma, is a kind of important biocontrol microorganisms, and the various plants fungal disease is all had the better prevention effect.The biochemical mechanism of Trichoderma biological and ecological methods to prevent plant disease, pests, and erosion effect mainly comprises: multiple extracellular enzymes such as metabolism chitinase, beta-1,3-glucanase, protease, and the cell wall of intense hydrolysis plant pathogenic fungi, thus kill pathogen; The degradative fungi cell wall discharges derivant, excites the defense reaction of plant; Metabolism produces antibiotic class materials such as pentanone, octanone, terpene, amino acid derivativges, trichodermin, gliotoxin, viridin, antibacterial peptide, suppresses growth of pathogenic bacteria; Produce the exciton of plant defense reaction, the inducing plant disease resistance; Strengthen plant tissue peroxidase, superoxide dismutase and catalase, polyphenol oxidase activity, diaphragm fat is not oxidized; Toxigenicity albumen, or, suppress pathogen growth and breeding with the joint action of antagonism of number of mechanisms.
The medium that the Trichoderma production method as the biological and ecological methods to prevent plant disease, pests, and erosion agent of bibliographical information is adopted is based on grain.As: (publication number: disclose the production method of a kind of Trichoderma CN 1554242A), its medium mainly is millet, rice, corn, barley, wheat or the jowar of particle diameter between the 1-5 millimeter to Chinese patent.(publication number: disclose the production method of a kind of Trichoderma CN 1422946A), its raw materials for production mainly are barley, wheat bran, naked oats etc. to Chinese patent.And the method for producing Trichoderma biological and ecological methods to prevent plant disease, pests, and erosion agent as medium with high concentrated organic wastewater yet there are no report.High concentrated organic wastewater from fermentation industry or food service industry can provide multiple nutrients for the Trichoderma growth.Therefore, produce Trichoderma biological and ecological methods to prevent plant disease, pests, and erosion agent with high concentrated organic wastewater and not only can reduce production costs, and can reduce sewage discharge.
Summary of the invention
The purpose of this invention is to provide a kind of method of utilizing high concentrated organic wastewater (as: waste water of fermentation industry, food-processing industry) to produce Trichoderma biological and ecological methods to prevent plant disease, pests, and erosion agent.
The production method of biocontrol trichoderma agent of the present invention is as follows:
1, seed culture: bacterial classification adopts conventional biological and ecological methods to prevent plant disease, pests, and erosion bacterial classification (as: Trichoderma viride (Trichoderma viride), Trichoderma harzianum (Trichoderma harziamum), yellow green trichoderma (Trichoderma auxeoviride), koning trichoderma (Trichodermakoningii) etc.), be inoculated in potato-dextrose culture-medium, or be inoculated in the sterilized wastewater of nutrition comparison balance, 20-30 ℃, 150-200rpm shake-flask culture 1-2 days.
2, liquid state fermentation: organic waste water is regulated pH about 5.0, add in the fermentation tank, necessity makes replenishes the small amounts of inorganic nutritive element, sterilization.With the inoculum concentration that cultured liquid seeds is pressed 5%-10%, be inoculated in the sterilized wastewater, logical filtrated air aerobic fermentation 1-3 days has a large amount of mycelia to produce in the waste water.
3, solid state fermentation: with the waste water of liquid state fermentation, add according to 1-4 times of weight in the useless biomass materials such as the stalk that crushes, bagasse, sugar beet pulp, stir, in 20-30 ℃ of static culture 2-5 days.These loose biomass materials both can play the holder effect, can provide part carbon source and a small amount of other nutrition for Trichoderma produces again, thereby produce a large amount of dense spores.The mould spore of wood is than the biological and ecological methods to prevent plant disease, pests, and erosion agent that is easier to store.
4, post processing: after cultured material drying is handled, add stabilizing agent and auxiliary agent, pulverize, packing.Also can directly be used in agricultural land soil according to the region situation, can effectively prevent and treat silborne fungal diseases, can improve the soil again, increase soil fertility.
The biocontrol trichoderma agent that technical solution of the present invention is produced can the multiple fungi plant disease of high-efficiency prevention and control, promotes plant growing, and nuisanceless, and production cost is low.Simultaneously for handling and utilizing high concentrated organic wastewater to find a kind of cost-effective new way.Below describe enforcement of the present invention in detail by specific embodiment, purpose is to help the reader to understand spiritual essence of the present invention better, but not as to the qualification of the scope of the present invention.
Embodiment 1
Gourmet powder waste water and maize straw fermentation Trichoderma viride:
With the potato waste water of starch-containing amount about 2% in 115 ℃ of sterilizations 15 minutes, inoculation Trichoderma viride bacterial classification, in 26 ℃, 180rpm shake-flask culture 2 days obtains liquid seeds.Liquid seeds is inoculated in the gourmet powder waste water of pH 5.0 according to 10% inoculum concentration, and logical filtrated air aerobic fermentation 2 days has a large amount of mycelia to produce in the waste water.Cultured waste water is added in the maize straw through pulverizing according to 3 times of weight, stir,, stir material in case of necessity one time in 26 ℃ of static culture 5 days.Cultured material spore content can reach 5.0 * 10
9Individual spore/gram substrate after drying is handled, adds stable and auxiliary agent, pulverizes packing.
Embodiment 2
Potato waste water and bagasse fermentation Trichoderma harzianum:
With the potato waste water of starch-containing amount about 2% in 115 ℃ of sterilizations 15 minutes, inoculation Trichoderma harzianum bacterial classification, in 26 ℃, 180rpm shake-flask culture 2 days obtains liquid seeds.Liquid seeds is inoculated in the potato waste water of pH 5.0 according to 6% inoculum concentration, and logical filtrated air aerobic fermentation 2 days has a large amount of mycelia to produce in the waste water.Cultured waste water is added in the bagasses according to 3 times of weight, stir,, stir material in case of necessity one time in 26 ℃ of static culture 5 days.Cultured material spore content can reach 6.5 * 10
9Individual spore/gram substrate after drying is handled, adds stable and auxiliary agent, pulverizes packing.
Embodiment 3
Potato waste water and sugar beet pulp close fermentation Trichoderma harzianum and koning trichoderma:
The potato waste water of starch-containing amount about 2% in 115 ℃ of sterilizations 15 minutes, is inoculated Trichoderma harzianum and koning trichoderma bacterial classification respectively, and in 26 ℃, 180rpm shake-flask culture 2 days obtains liquid seeds.Liquid seeds is inoculated in the potato waste water of pH 5.0 according to 6% inoculum concentration, and logical filtrated air aerobic fermentation 2 days has a large amount of mycelia to produce in the waste water.Cultured waste water is added in the sugar beet pulps according to 2.5 times of weight, stir,, stir material in case of necessity one time in 26 ℃ of static culture 6 days.Cultured material spore content can reach 9.1 * 10
9Individual spore/gram substrate after drying is handled, adds stable and auxiliary agent, pulverizes packing.
Claims (3)
1. method of utilizing high concentrated organic wastewater to produce biocontrol trichoderma agent, it is characterized in that, this method is that the high concentrated organic wastewater that produces with fermentation industry and food-processing industry is a main production raw material, adopts the method for liquid and solid-state combined ferment to produce biocontrol trichoderma agent.
2. the production method of the described biocontrol trichoderma agent of claim 1, it is characterized in that, the Trichoderma kind is the conventional Trichoderma that is used for the biological and ecological methods to prevent plant disease, pests, and erosion agent, as: Trichoderma viride (Trichoderma viride), Trichoderma harzianum (Trichoderma harziamum), yellow green trichoderma (Trichoderma auxeoviride), koning trichoderma (Trichoderma koningii).
3. the production method of the described biocontrol trichoderma agent of claim 1 is characterized in that, carries out seed culture with the medium of nutrient component equilibrium; Being inoculated in high concentrated organic wastewater is main medium, carries out liquid state and cultivates; The waste water of liquid culture mixes with abandoned biomass and carries out solid state fermentation; The tunning drying is handled the back and is added stabilizing agent and auxiliary agent, pulverizes, packs, stores, or directly be used in the farmland controlling plant diseases.
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2006101655951A CN101204159A (en) | 2006-12-22 | 2006-12-22 | Biological control agents for plant diseases extracting from high concentration organic wastewater |
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| CNA2006101655951A CN101204159A (en) | 2006-12-22 | 2006-12-22 | Biological control agents for plant diseases extracting from high concentration organic wastewater |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102040403A (en) * | 2009-10-10 | 2011-05-04 | 中国科学院生态环境研究中心 | Production of trichoderma spp. microbial fertilizer by waste water of kitchen garbage |
| CN102050645A (en) * | 2009-10-27 | 2011-05-11 | 中国科学院生态环境研究中心 | Method for producing trichoderma microbial fertilizer by utilizing sweet potato starch wastewater |
| CN102199051A (en) * | 2010-03-23 | 2011-09-28 | 中国科学院生态环境研究中心 | Trichoderma microbial fertilizer production by reutilizing livestock and poultry manure |
| CN103931660A (en) * | 2013-01-17 | 2014-07-23 | 无锡中科活力生物技术有限公司 | Method utilizing critic acid wastewater to produce bacillus amyloliquefaciens bio-control inoculant |
| CN109868243A (en) * | 2019-01-30 | 2019-06-11 | 中国环境科学研究院 | Accelerate the microbial bacterial agent of organic pollution of soil reparation, preparation method and application |
-
2006
- 2006-12-22 CN CNA2006101655951A patent/CN101204159A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102040403A (en) * | 2009-10-10 | 2011-05-04 | 中国科学院生态环境研究中心 | Production of trichoderma spp. microbial fertilizer by waste water of kitchen garbage |
| CN102050645A (en) * | 2009-10-27 | 2011-05-11 | 中国科学院生态环境研究中心 | Method for producing trichoderma microbial fertilizer by utilizing sweet potato starch wastewater |
| CN102199051A (en) * | 2010-03-23 | 2011-09-28 | 中国科学院生态环境研究中心 | Trichoderma microbial fertilizer production by reutilizing livestock and poultry manure |
| CN103931660A (en) * | 2013-01-17 | 2014-07-23 | 无锡中科活力生物技术有限公司 | Method utilizing critic acid wastewater to produce bacillus amyloliquefaciens bio-control inoculant |
| CN103931660B (en) * | 2013-01-17 | 2018-08-24 | 无锡中科活力生物技术有限公司 | Bacillus amyloliquefaciens biocontrol agent is produced using citric acid wastewater |
| CN109868243A (en) * | 2019-01-30 | 2019-06-11 | 中国环境科学研究院 | Accelerate the microbial bacterial agent of organic pollution of soil reparation, preparation method and application |
| CN109868243B (en) * | 2019-01-30 | 2022-02-01 | 中国环境科学研究院 | Microbial agent for accelerating soil organic matter pollution remediation, preparation method and application thereof |
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Open date: 20080625 |