CN102763683A - Biological bactericidal disinfectant and preparation method thereof - Google Patents
Biological bactericidal disinfectant and preparation method thereof Download PDFInfo
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- CN102763683A CN102763683A CN2012102314470A CN201210231447A CN102763683A CN 102763683 A CN102763683 A CN 102763683A CN 2012102314470 A CN2012102314470 A CN 2012102314470A CN 201210231447 A CN201210231447 A CN 201210231447A CN 102763683 A CN102763683 A CN 102763683A
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- lactic acid
- brown sugar
- lactobacillus acidophilus
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Abstract
The invention provides a biological bactericidal disinfectant and a preparation method thereof. The biological bactericidal disinfectant consists of the following components in part by mass: 1 to 2 parts of chitosan, 1.5 to 2.5 parts of acetic acid, 2 to 3 parts of lactic acid, 1 to 2 parts of lactobacillus acidophilus, 5 to 10 parts of brown sugar and 100 to 200 parts of water. The method comprises the following steps of: mixing the chitosan, the water and the acetic acid, heating, dissolving and cooling to obtain a chitosan solution; mixing the brown sugar, the lactic acid and the water, heating, dissolving, keeping temperature, cooling, adding lactobacillus acidophilus, regulating the pH value to be 5.5 to 6.5 by using the lactic acid, and fermenting for 10 to 12 hours to obtain bacterium liquor in which the bacterial counts are 1*10<8> to 1*10<9> colony forming units (cfu)/gram; and mixing the bacterium liquor and the chitosan solution uniformly, and regulating the pH value to be 3 to 3.5 by using the lactic acid to obtain the biological bactericidal disinfectant. When sewage is treated by using the biological bactericidal disinfectant, the propagation of beneficial bacterial colonies can be promoted, endotoxin can be eliminated, and harmful bacteria can be killed or controlled, so that the ecological balance of a water body is facilitated.
Description
Technical field
The present invention relates to a kind of biological antibiotic disinfectant, especially a kind of disinfection sanitizer that is specially adapted to dispose of sewage belongs to biological antibiotic disinfectant technical field.
Background technology
Existing chemical sterilization disinfectant; Though have very strong sterilizing effect; But in sewage purifications such as some ecotope such as water circulation use system, river basin, lake, pond are handled, but exist more negative effect, promptly in killing water body in the noxious bacteria; Also can damage aquatic animal, plant and microorganism in the water body; Especially numerous profitable strains also can be injured by the chemical sterilization disinfectant, and this is totally unfavorable to the water ecology balance, very easily cause the water ecology imbalance.Along with people's improves constantly environmental quality requirement; People hope to obtain a kind ofly can not destroy water ecological setting; And can give full play to the advantage effect of profitable strain in the water body, kill harmful flora simultaneously, safety in utilization, the biological antibiotic disinfectant that reliability is high; Remedying the defective of existing chemical sterilization disinfectant, and then administer and the water of life of protection human survival.
Summary of the invention
It is a kind of for solving negative effect and the harm that existing chemical sterilization disinfectant causes water ecological setting that the present invention provides, and the profitable strain in the protection water body is kept the water ecology balance, biological antibiotic disinfectant and preparation thereof.
The present invention realizes through following technical proposal: a kind of biological antibiotic disinfectant is characterized in that being made up of the component of following mass parts:
1～2 part of shitosan, 1.5～2.5 parts of acetic acid,
2～3 parts of lactic acid, 1～2 part of lactobacillus acidophilus,
5～10 parts in brown sugar, 100～200 parts in water.
Biological antibiotic disinfectant provided by the invention prepares through following method:
(1) get the raw materials ready by the component of following mass parts:
1～2 part of shitosan, 1.5～2.5 parts of acetic acid,
2～3 parts of lactic acid, 1～2 part of lactobacillus acidophilus,
5～10 parts in brown sugar, 100～200 parts in water;
(2) step (1) is equipped with shitosan and adds in the suitable quantity of water, dispersed with stirring evenly back adds acetic acid, is heated to 50～80 ℃, and under agitation is dissolved to transparent no particle, is cooled to 25～30 ℃, gets chitosan solution;
(3) step (1) is equipped with brown sugar and joins in the remaining water, be heated to 80 ~ 85 ℃, make the brown sugar dissolving; Be incubated after 20 ~ 30 minutes, be cooled to 30～40 ℃ again, add lactobacillus acidophilus; And get an amount of lactic acid the pH value is transferred to 5.5～6.5, fermented 10～12 hours, obtaining viable count is 1 * 10
8～1 * 10
9The bacterium liquid of cfu/g;
(4) the bacterium liquid of step (3) and the chitosan solution of step (2) are mixed, adding remaining lactic acid mixing adjusting pH value is 3～3.5, promptly obtains the biological antibiotic disinfectant.
Said lactobacillus acidophilus (Lacidophilus) is commercial bacterium.
Said shitosan, acetic acid, lactic acid are commercial product.
The present invention has advantage and effect: when the biological antibiotic disinfectant that adopts such scheme to obtain is disposed of sewage; Can eliminate the harmful endotoxin in the water body, and can suppress the noxious bacteria in the water body in killing water body in the noxious bacteria; Let the profitable strain in the water body expanded numerous in a large number; Beneficial bacterium is occupied surgingly draw, make aquatic animal, plant and the microorganism etc. in the water body can healthy growth, to keep and to improve the water ecology balance; After improvement sewage is obtained effect, effectively protect the ecotope of water resource.
Embodiment
Below in conjunction with embodiment the present invention is further specified.
Embodiment 1
(1) get the raw materials ready by the component of following mass parts:
2 parts of shitosans, 1.5 parts of acetic acid,
2.5 parts of lactic acid, 2 parts of lactobacillus acidophiluss,
8 parts in brown sugar, 200 parts in water;
(2) step (1) is equipped with shitosan and adds in the suitable quantity of water, dispersed with stirring evenly back adds acetic acid, is heated to 50 ℃, and under agitation is dissolved to transparent no particle, is cooled to 25 ℃, gets chitosan solution;
(3) step (1) is equipped with brown sugar and joins in the remaining water, be heated to 80 ℃, make the brown sugar dissolving, be incubated after 20 minutes, be cooled to 30 ℃ again, the adding lactobacillus acidophilus, and get an amount of lactic acid the pH value is transferred to 5.5, fermented 10 hours, obtaining viable count is 1 * 10
8The bacterium liquid of cfu/g;
(4) the bacterium liquid of step (3) and the chitosan solution of step (2) are mixed, adding remaining lactic acid mixing adjusting pH value is 3, promptly obtains the biological antibiotic disinfectant.
Embodiment 2
(1) get the raw materials ready by the component of following mass parts:
1 part of shitosan, 2.0 parts of acetic acid,
2 parts of lactic acid, 1.5 parts of lactobacillus acidophiluss,
10 parts in brown sugar, 150 parts in water;
(2) step (1) is equipped with shitosan and adds in the suitable quantity of water, dispersed with stirring evenly back adds acetic acid, is heated to 80 ℃, and under agitation is dissolved to transparent no particle, is cooled to 30 ℃, gets chitosan solution;
(3) step (1) is equipped with brown sugar and joins in the remaining water, be heated to 85 ℃, make the brown sugar dissolving, be incubated after 30 minutes, be cooled to 40 ℃ again, the adding lactobacillus acidophilus, and get an amount of lactic acid the pH value is transferred to 6.5, fermented 12 hours, obtaining viable count is 1 * 10
9The bacterium liquid of cfu/g;
(4) the bacterium liquid of step (3) and the chitosan solution of step (2) are mixed, adding remaining lactic acid mixing adjusting pH value is 3.5, promptly obtains the biological antibiotic disinfectant.
Embodiment 3
(1) get the raw materials ready by the component of following mass parts:
1.5 parts of shitosans, 2.5 parts of acetic acid,
3 parts of lactic acid, 1 part of lactobacillus acidophilus,
5 parts in brown sugar, 100 parts in water;
(2) step (1) is equipped with shitosan and adds in the suitable quantity of water, dispersed with stirring evenly back adds acetic acid, is heated to 65 ℃, and under agitation is dissolved to transparent no particle, is cooled to 28 ℃, gets chitosan solution;
(3) step (1) is equipped with brown sugar and joins in the remaining water, be heated to 82 ℃, make the brown sugar dissolving, be incubated after 25 minutes, be cooled to 35 ℃ again, the adding lactobacillus acidophilus, and get an amount of lactic acid the pH value is transferred to 6, fermented 11 hours, obtaining viable count is 1 * 10
9The bacterium liquid of cfu/g;
(4) the bacterium liquid of step (3) and the chitosan solution of step (2) are mixed, adding remaining lactic acid mixing adjusting pH value is 3.2, promptly obtains the biological antibiotic disinfectant.
Prove effect of the present invention through comparative trial below:
1) gets two parts of 100 liters of sewage, be respectively charged in A, two buckets of B;
2) in the sewage of A bucket, press 5/10000ths of sewage weight, add the biological antibiotic disinfectant that the embodiment of the invention 2 provides, mixing;
3) adding conventional chemical disinfectant is an amount of in the sewage of B bucket, mixing;
4) after two hours, the stink that A bucket water body gives out obviously reduces, and simultaneously impurity is flocculated agglomeratingly in the water, and it is limpid that water quality becomes, water intaking test after 8 hours, and the result shows: the quantity of noxious bacteria has reduced 70% in the A bucket water, and beneficial bacterium increases to some extent;
5) the various bacterium of water are killed in the B bucket, and stink obviously reduces, but do not see the impurity flocculation, and water quality is still muddy.
Result of the test proves: biological antibiotic disinfectant provided by the invention can make in the water harmful bacterium, putrefying bacteria, impurity significantly reduce, and beneficial bacterium then increases to some extent.
Claims (3)
1. biological antibiotic disinfectant, it is characterized in that by 1.5～2.5 parts of 1～2 part of component shitosan, the acetic acid of following mass parts,
2～3 parts of lactic acid, 1～2 part of lactobacillus acidophilus,
5～10 parts in brown sugar, 100～200 parts in water.
2. the preparation method of a biological antibiotic disinfectant is characterized in that through following each step:
1) get the raw materials ready by the component of following mass parts:
1～2 part of shitosan, 1.5～2.5 parts of acetic acid,
2～3 parts of lactic acid, 1～2 part of lactobacillus acidophilus,
5～10 parts in brown sugar, 100～200 parts in water;
2) shitosan that step 1) is equipped with is added in the suitable quantity of water, dispersed with stirring evenly back adds acetic acid, is heated to 50～80 ℃, and under agitation is dissolved to transparent no particle, is cooled to 25～30 ℃, gets chitosan solution;
3) brown sugar that step 1) is equipped with is joined in the remaining water, be heated to 80 ~ 85 ℃, make the brown sugar dissolving; Be incubated after 20 ~ 30 minutes, be cooled to 30～40 ℃ again, add lactobacillus acidophilus; With an amount of lactic acid the pH value is transferred to 5.5～6.5, fermented 10～12 hours, obtaining viable count is 1 * 10
8～1 * 10
9The bacterium liquid of cfu/g;
4) with the bacterium liquid and the step 2 of step 3)) chitosan solution mix, adding remaining newborn acid for adjusting pH value is 3～3.5, promptly obtains the biological antibiotic disinfectant.
3. according to the preparation method of the said biological antibiotic disinfectant of claim 2, it is characterized in that: said lactobacillus acidophilus is commercial product.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012102314470A CN102763683A (en) | 2012-07-05 | 2012-07-05 | Biological bactericidal disinfectant and preparation method thereof |
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| CN2012102314470A CN102763683A (en) | 2012-07-05 | 2012-07-05 | Biological bactericidal disinfectant and preparation method thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105883988A (en) * | 2016-05-20 | 2016-08-24 | 张能力 | Sewage purification disinfectant and preparation method thereof |
| CN106818744A (en) * | 2017-02-23 | 2017-06-13 | 江苏省农业科学院 | Disinfectant for abolishing bacterial biofilm |
| CN106915805A (en) * | 2017-03-28 | 2017-07-04 | 安徽中煦环保新材料科技有限公司 | A kind of sanitary sewage disinfectant and preparation method thereof |
| CN113331212A (en) * | 2021-07-10 | 2021-09-03 | 江苏姚氏环保技术有限公司 | Sterilization algicide for coking sewage treatment and preparation method thereof |
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| CN101050423A (en) * | 2007-03-19 | 2007-10-10 | 上海真美科技发展有限公司 | Composite preparation microbiological, and preparation method |
| CN101269227A (en) * | 2007-03-21 | 2008-09-24 | 北京世纪星伊埃姆生物技术研究所 | Biological agent for purifying air and preparation method thereof |
| CN101468839A (en) * | 2007-12-28 | 2009-07-01 | 黄庆禄 | Preparation of sewerage ecological purificant |
| CN102107951A (en) * | 2009-12-24 | 2011-06-29 | 北京金源化学集团有限公司 | Microbialactivity enhancing liquid agent for sewage treatment |
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2012
- 2012-07-05 CN CN2012102314470A patent/CN102763683A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101050423A (en) * | 2007-03-19 | 2007-10-10 | 上海真美科技发展有限公司 | Composite preparation microbiological, and preparation method |
| CN101269227A (en) * | 2007-03-21 | 2008-09-24 | 北京世纪星伊埃姆生物技术研究所 | Biological agent for purifying air and preparation method thereof |
| CN101468839A (en) * | 2007-12-28 | 2009-07-01 | 黄庆禄 | Preparation of sewerage ecological purificant |
| CN102107951A (en) * | 2009-12-24 | 2011-06-29 | 北京金源化学集团有限公司 | Microbialactivity enhancing liquid agent for sewage treatment |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105883988A (en) * | 2016-05-20 | 2016-08-24 | 张能力 | Sewage purification disinfectant and preparation method thereof |
| CN106818744A (en) * | 2017-02-23 | 2017-06-13 | 江苏省农业科学院 | Disinfectant for abolishing bacterial biofilm |
| CN106818744B (en) * | 2017-02-23 | 2019-05-24 | 江苏省农业科学院 | For abolishing the disinfectant of bacterial biofilm |
| CN106915805A (en) * | 2017-03-28 | 2017-07-04 | 安徽中煦环保新材料科技有限公司 | A kind of sanitary sewage disinfectant and preparation method thereof |
| CN113331212A (en) * | 2021-07-10 | 2021-09-03 | 江苏姚氏环保技术有限公司 | Sterilization algicide for coking sewage treatment and preparation method thereof |
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Effective date of abandoning: 20121107 |
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