CN103463654A - Method for producing deodorant microbial agent - Google Patents

Method for producing deodorant microbial agent Download PDF

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Publication number
CN103463654A
CN103463654A CN2013104425044A CN201310442504A CN103463654A CN 103463654 A CN103463654 A CN 103463654A CN 2013104425044 A CN2013104425044 A CN 2013104425044A CN 201310442504 A CN201310442504 A CN 201310442504A CN 103463654 A CN103463654 A CN 103463654A
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parts
microorganism
bacillus pumilus
rhodopseudomonas palustris
concentration
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CN2013104425044A
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CN103463654B (en
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李佩佩
王鹏
刘鲁民
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Qingdao Vland Biotech Group Co Ltd
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Qingdao Vland Biotech Group Co Ltd
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Abstract

The invention provides a method for producing a deodorant microbial agent. The method comprises the following steps: carrying out high-density fermentation on streptococcus thermophilus, pseudomonas putida, rhodopseudomonas palustris, bacillus pumilus and enterococcus faecium, and then freezing and drying; adding a sodium citrate solution of which the concentration is 8-10% according to 1-5% of mass-volume ratio after mixing according to the ratio, so as to prepare a liquid deodorant product. The concentration of bad smells such as an ammonia gas, hydrogen sulfide and the like in the air in the places such as a toilet, a farm and a wasteyard can be effectively reduced; an odor-generating element is also restrained, so that the air quality and surroundings of people are significantly improved. The production method disclosed by the invention is simple, and adapts to most of existing fermentation technologies and equipments; large-scale popularization and application are facilitated.

Description

A kind of method of producing the deodorizing microorganism preparation
Technical field
The present invention relates to technical field of microbe application, be specifically related to a kind of deodorizing microorganism preparation and preparation method thereof.
Background technology
Odor pollution is one of seven universally acknowledged overall situation public hazards.Common odorant pollutant has ammonia, trimethylamine, hydrogen sulfide, methanthiol, methyl disulfide, Carbon bisulfide and styrene etc., be mainly derived from industrial and agricultural production department and people's life, such as farming and animal husbandry production with in processing, the excrement of generation is smelly, fish is smelly, corruption is smelly, decayed fruit is smelly, wild herbs are smelly etc.The sulfide produced in petrochemical production process, hydro carbons, benzene class, phenols and Ammonia and tar, Colophonium steam, ammonia and various organic solvent, and the stench of urban public utilities.The odorant pollutant of high concentration has direct harm to health.
At present, can be divided into chemical deodorizing and biological deodorizing two broad aspect to the stench removal methods, the chemical deodorizing principle is, by outer source strength oxidation material, the stench composition is carried out to oxidative degradation, closely eliminate stench, though it is with strong points, instant effect, but its range of application is little, while running into the complex effluvium gas ingredients, deodorization usefulness will lose efficacy, and after using the chemical deodorizing agent, residual chemical substance tends to cause secondary pollution.The biological deodorizing principle is to remove stench by external source function strain or enzyme to the direct degraded of stench or to the inhibition of producing the stench microorganism.The characteristics such as biological deodorizing can be eliminated stench lastingly, and non-secondary pollution is easy to use, with low cost, become the study hotspot that stench is controlled, and finds effective, green biological deodorizing product and method, becomes the urgent needs of present society.
Summary of the invention
The present invention, for solving the prior art problem, provides a kind of odorant pollutant in air of can removing, and then the complex microorganism preparations purified air.Said preparation, by the collaborative facilitation of multiple-microorganism, can effectively suppress to produce smelly element, the degraded odorant pollutant, thus the concentration of minimizing harmful gas reduces odor concentration.
The applicant finds by long-term screening study, can produce synergism between streptococcus thermophilus, pseudomonas putida, Rhodopseudomonas palustris, Bacillus pumilus and enterococcus faecalis, effectively reduce the concentration of the foul smell such as Ammonia in Air gas and hydrogen sulfide, thereby facilitate the present invention.
One aspect of the present invention provides a kind of deodorizing microorganism preparation, by streptococcus thermophilus ( streptococcus thermophilus), pseudomonas putida ( pseudomonas putida), Rhodopseudomonas palustris ( rhodopseudomonas palustris), Bacillus pumilus ( bacillus pumilus) and enterococcus faecalis ( enterococcus faecium) form.
The preferred streptococcus thermophilus CGMCC1.3996 of each component, pseudomonas putida CGMCC1.2309, Rhodopseudomonas palustris CGMCC1.2349, Bacillus pumilus CGMCC1.8112 and enterococcus faecalis CGMCC1.2136 in above-mentioned deodorizing microorganism preparation.
In above-mentioned deodorizing microorganism preparation, each component and content thereof are respectively: streptococcus thermophilus 40-60 part, pseudomonas putida 35-40 part, Rhodopseudomonas palustris 18-30 part, Bacillus pumilus 25-50 part and enterococcus faecalis 45-80 part.
In above-mentioned deodorizing microorganism preparation, the content of each component is preferably respectively: 55 parts of 45 parts of streptococcus thermophiluss, 38 parts of pseudomonas putidas, 25 parts of Rhodopseudomonas palustris, 40 parts of Bacillus pumilus and enterococcus faecalis.
The present invention provides the preparation method of above-mentioned deodorizing microorganism preparation on the other hand, comprises the steps:
1) streptococcus thermophilus CGMCC1.3996, pseudomonas putida CGMCC1.2309, Rhodopseudomonas palustris CGMCC1.2349, Bacillus pumilus CGMCC1.8112 and enterococcus faecalis CGMCC1.2136 are adopted respectively to the high density liquid fermentation, by after the fermentation liquid lyophilizing, make spore content up to 10 10-10 11the ultra concentration mycopowder of CFU/g;
2) the ultra concentration mycopowder is mixed with to complex microorganism preparations according to streptococcus thermophilus 40-60 part, pseudomonas putida 35-40 part, Rhodopseudomonas palustris 18-30 part, Bacillus pumilus 25-50 part and enterococcus faecalis 45-80 part, is solid deodorizing microorganism preparation;
3) get step 2) microorganism formulation that makes, add by the 1-5% mass volume ratio sodium citrate solution that concentration is 8-10%, mix homogeneously, obtain liquid deodorant microorganism formulation of the present invention.
The present invention provides the application of above-mentioned deodorizing microorganism preparation in purifying air on the other hand.
Aforesaid liquid deodorizing microorganism preparation is sprayed on to the high places of malodorant concentration such as lavatory, plant, soot, sprays 2-3 time weekly, each consumption 100-200 mL/ m 3.
beneficial effect
Complex microorganism preparations provided by the invention is by the synergism between streptococcus thermophilus, pseudomonas putida, Rhodopseudomonas palustris, Bacillus pumilus and enterococcus faecalis, can effectively reduce the concentration of the foul smell such as the airborne ammonia in the places such as lavatory, plant, soot and hydrogen sulfide, suppress to produce smelly element, thereby significantly improve air quality and people's living environment.Production method provided by the invention is simple, is adapted to the existing fermentation technology of great majority and equipment, is conducive to large-scale promotion application.
The specific embodiment
Various reagent and the equipment that the present invention relates to, all can be selected from commercially available any one; The present invention relates in bacterial strain streptococcus thermophilus purchased from Chinese common micro-organisms preservation administrative center, deposit number CGMCC1.3996; Pseudomonas putida is purchased from Chinese common micro-organisms preservation administrative center, deposit number CGMCC1.2309; Rhodopseudomonas palustris is Qingdao Weilan Biology Group Co., Ltd.'s patented strain, deposit number CGMCC1.2349; Bacillus pumilus is purchased from Chinese common micro-organisms preservation administrative center, deposit number CGMCC1.8112; Enterococcus faecalis is purchased from Chinese common micro-organisms preservation administrative center, deposit number CGMCC1.2136.
Below in conjunction with specific embodiment, further set forth the present invention.
embodiment 1
A kind of deodorizing microorganism preparation, streptococcus thermophilus, pseudomonas putida, Rhodopseudomonas palustris, Bacillus pumilus and enterococcus faecalis, consist of, each component and content thereof are respectively: 45 parts of 40 parts of streptococcus thermophiluss, 40 parts of pseudomonas putidas, 30 parts of Rhodopseudomonas palustris, 25 parts of Bacillus pumilus and enterococcus faecalis.
The preparation method of above-mentioned deodorizing microorganism preparation, comprise the steps:
1) streptococcus thermophilus, pseudomonas putida, Rhodopseudomonas palustris, Bacillus pumilus and enterococcus faecalis are adopted respectively to the high density liquid fermentation, by after the fermentation liquid lyophilizing, make spore content up to 10 10-10 11the ultra concentration mycopowder of CFU/g;
2) the ultra concentration mycopowder is mixed with to complex microorganism preparations according to 45 parts of 40 parts of streptococcus thermophiluss, 40 parts of pseudomonas putidas, 30 parts of Rhodopseudomonas palustris, 25 parts of Bacillus pumilus and enterococcus faecalis, is solid deodorizing microorganism preparation;
3) get step 2) microorganism formulation that makes, add by 1% mass volume ratio the sodium citrate solution that concentration is 10%, mix homogeneously, obtain liquid deodorant microorganism formulation of the present invention.
embodiment 2
A kind of deodorizing microorganism preparation, streptococcus thermophilus, pseudomonas putida, Rhodopseudomonas palustris, Bacillus pumilus and enterococcus faecalis, consist of, each component and content thereof are respectively: 80 parts of 60 parts of streptococcus thermophiluss, 35 parts of pseudomonas putidas, 18 parts of Rhodopseudomonas palustris, 50 parts of Bacillus pumilus and enterococcus faecalis.
The preparation method of above-mentioned deodorizing microorganism preparation, comprise the steps:
1) streptococcus thermophilus, pseudomonas putida, Rhodopseudomonas palustris, Bacillus pumilus and enterococcus faecalis are adopted respectively to the high density liquid fermentation, by after the fermentation liquid lyophilizing, make spore content up to 10 10-10 11the ultra concentration mycopowder of CFU/g;
2) the ultra concentration mycopowder is mixed with to complex microorganism preparations according to 80 parts of 60 parts of streptococcus thermophiluss, 35 parts of pseudomonas putidas, 18 parts of Rhodopseudomonas palustris, 50 parts of Bacillus pumilus and enterococcus faecalis, is solid deodorizing microorganism preparation;
3) get step 2) microorganism formulation that makes, add by 5% mass volume ratio the sodium citrate solution that concentration is 8%, mix homogeneously, obtain liquid deodorant microorganism formulation of the present invention.
embodiment 3
A kind of deodorizing microorganism preparation, streptococcus thermophilus, pseudomonas putida, Rhodopseudomonas palustris, Bacillus pumilus and enterococcus faecalis, consist of, each component and content thereof are respectively: 55 parts of 45 parts of streptococcus thermophiluss, 38 parts of pseudomonas putidas, 25 parts of Rhodopseudomonas palustris, 40 parts of Bacillus pumilus and enterococcus faecalis.
The preparation method of above-mentioned deodorizing microorganism preparation, comprise the steps:
1) streptococcus thermophilus, pseudomonas putida, Rhodopseudomonas palustris, Bacillus pumilus and enterococcus faecalis are adopted respectively to the high density liquid fermentation, by after the fermentation liquid lyophilizing, make spore content up to 10 10-10 11the ultra concentration mycopowder of CFU/g;
2) the ultra concentration mycopowder is mixed with to complex microorganism preparations according to 55 parts of 45 parts of streptococcus thermophiluss, 38 parts of pseudomonas putidas, 25 parts of Rhodopseudomonas palustris, 40 parts of Bacillus pumilus and enterococcus faecalis, is solid deodorizing microorganism preparation;
3) get step 2) microorganism formulation that makes, add by 4% mass volume ratio the sodium citrate solution that concentration is 9%, mix homogeneously, obtain liquid deodorant microorganism formulation of the present invention.
the application of embodiment 4 deodorizing microorganism preparations
1, add 100g solid-state deodorizing microorganism preparation of the present invention in the beaker that the fresh pig manure of 1000g is housed (2L), and after feces mixes, the preservative film sealed beaker; Do the contrast that does not add microbial inoculum, every group of experiment arranges three repetitions, measures stench intensity, ammonia concentration and concentration of hydrogen sulfide after 7 days simultaneously, and measurement result is as shown in table 1 below:
Testing index Matched group Add the microorganism formulation group Reduction rate
Odor strength 297312 178387 40.0%
Ammonia concentration 42.3 21.4 49.4%
Concentration of hydrogen sulfide 156.1 77.2 50.5%
As can be seen from Table 1, after adding deodorizing microorganism preparation of the present invention, the ammonia that feces produces and the concentration of hydrogen sulfide have reduced respectively 49.4% and 50.5%, significantly reduce odor strength, illustrate that microorganism formulation of the present invention has good deodorizing effect, can effectively improve air quality.
2, choose in the other life refuse collecting of Li Cunhe and deposit a little as test site, detect respectively spray liquid deodorant microorganism formulation of the present invention before and sprinkling (sprinkling consumption 150g/ m 3) rear the 3rd day airborne stench intensity, ammonia concentration and concentration of hydrogen sulfide, measurement result is as shown in table 2 below:
Testing index Before sprinkling After sprinkling Reduction rate
Odor strength 312410 164371 47.4%
Ammonia concentration 56.1 27.8 50.4%
Concentration of hydrogen sulfide 152.8 79.1 48.2%
As can be seen from Table 2, after spraying deodorizing microorganism of the present invention, in the soot air, odor strength significantly reduces by 47.4%, wherein ammonia concentration has reduced by 50.4%, concentration of hydrogen sulfide has reduced by 48.2%, the deodorizing effect that microorganism formulation of the present invention is described is remarkable, can effectively reduce the concentration of odorant in air, is conducive to better people's living environment.

Claims (6)

1. the production method of a deodorizing microorganism preparation, comprise the steps:
1) streptococcus thermophilus, pseudomonas putida, Rhodopseudomonas palustris, Bacillus pumilus and enterococcus faecalis are adopted respectively to the high density liquid fermentation, by after the fermentation liquid lyophilizing, make spore content up to 10 10-10 11the ultra concentration mycopowder of CFU/g;
2) the ultra concentration mycopowder is respectively to streptococcus thermophilus 40-60 part, pseudomonas putida 35-40 part, Rhodopseudomonas palustris 18-30 part, Bacillus pumilus 25-50 part and enterococcus faecalis 45-80 part according to mass ratio and is mixed with complex microorganism preparations, be solid deodorizing microorganism preparation;
3) get step 2) microorganism formulation that makes, to press the 1-5% mass volume ratio and add sodium citrate solution, mix homogeneously, obtain the liquid deodorant microorganism formulation.
2. production method as claimed in claim 1, it is characterized in that, described streptococcus thermophilus, pseudomonas putida, Rhodopseudomonas palustris, Bacillus pumilus and enterococcus faecalis are respectively streptococcus thermophilus CGMCC1.3996, pseudomonas putida CGMCC1.2309, Rhodopseudomonas palustris CGMCC1.2349, Bacillus pumilus CGMCC1.8112 and enterococcus faecalis CGMCC1.2136.
3. production method as claimed in claim 1, it is characterized in that step 2) mass ratio of described streptococcus thermophilus, pseudomonas putida, Rhodopseudomonas palustris, Bacillus pumilus and enterococcus faecalis is 55 parts of 45 parts of streptococcus thermophiluss, 38 parts of pseudomonas putidas, 25 parts of Rhodopseudomonas palustris, 40 parts of Bacillus pumilus and enterococcus faecalis.
4. production method as claimed in claim 1, is characterized in that, the concentration of the described sodium citrate solution of step 3) is 8-10%.
5. the deodorizing microorganism preparation that the described production method of above-mentioned arbitrary claim prepares.
6. the application of the described deodorizing microorganism preparation of claim 5.
CN201310442504.4A 2013-09-26 2013-09-26 A method of producing deodorant microbial agent Active CN103463654B (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106914128A (en) * 2017-04-28 2017-07-04 中林山水(北京)生态科技股份有限公司 A kind of biological deodorant and preparation method thereof
CN108179113A (en) * 2018-01-23 2018-06-19 黄永根 A kind of production hydrogen composite biological agent
CN108179112A (en) * 2018-01-23 2018-06-19 黄永根 The method of chlorella pyrenoidosa joint mushroom production hydrogen
CN111548960A (en) * 2020-05-13 2020-08-18 深圳市东荣生物科技有限责任公司 Compound microbial preparation for deodorization

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US4871539A (en) * 1984-01-17 1989-10-03 Seikenkai Foundational Juridical Person Biodeodorizer and process for preparing same
CN1059331A (en) * 1990-08-28 1992-03-11 东邦科技株式会社 The deodorizing method of dung and urine of breeding animals
JPH08298982A (en) * 1995-05-02 1996-11-19 Aasu Giken:Kk Complex microbial pharmaceutical preparation
CN101538538A (en) * 2008-03-18 2009-09-23 上海环伟生物科技有限公司 Composite microorganism viable bacteria preparation and preparation method and application of same
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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106914128A (en) * 2017-04-28 2017-07-04 中林山水(北京)生态科技股份有限公司 A kind of biological deodorant and preparation method thereof
CN108179113A (en) * 2018-01-23 2018-06-19 黄永根 A kind of production hydrogen composite biological agent
CN108179112A (en) * 2018-01-23 2018-06-19 黄永根 The method of chlorella pyrenoidosa joint mushroom production hydrogen
CN108179112B (en) * 2018-01-23 2021-05-18 杭州富阳佳畅机械有限公司 Method for producing hydrogen by chlorella pyrenoidosa combined bacteria
CN108179113B (en) * 2018-01-23 2021-05-18 杭州富阳佳畅机械有限公司 Hydrogen-producing composite biological agent
CN111548960A (en) * 2020-05-13 2020-08-18 深圳市东荣生物科技有限责任公司 Compound microbial preparation for deodorization

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