CN110156242A - The method of bacteria-algae synergism efficient process aquaculture wastewater - Google Patents
The method of bacteria-algae synergism efficient process aquaculture wastewater Download PDFInfo
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- CN110156242A CN110156242A CN201810048400.8A CN201810048400A CN110156242A CN 110156242 A CN110156242 A CN 110156242A CN 201810048400 A CN201810048400 A CN 201810048400A CN 110156242 A CN110156242 A CN 110156242A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/72—Treatment of water, waste water, or sewage by oxidation
- C02F1/78—Treatment of water, waste water, or sewage by oxidation with ozone
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F2001/007—Processes including a sedimentation step
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F2303/00—Specific treatment goals
- C02F2303/04—Disinfection
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F7/00—Aeration of stretches of water
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Abstract
The invention discloses a kind of methods of bacteria-algae synergism efficient process aquaculture wastewater, bacterium algae compounding combination processing livestock and poultry sewage, the ammonia nitrogen in livestock and poultry sewage is first specifically converted by nitrate nitrogen using autotrophic ammonia oxidizing bacterium, Autotrophic nitrification bacterium and allotrophic nitrobacteria synergistic effect, then will treated that sewage is passed through gas-lifting type column bioreactor, using the microalgae denitrification dephosphorization that deposit number is CCTCC NO:M 2017461 and carry out the accumulation of biomass.It has the beneficial effect that through microbial function bacterium and microalgae synergistic effect, quickly reduces the pollutants such as nitrogen, the phosphorus in livestock and poultry sewage, shorten hydraulic detention time, improve micro algae biomass accumulating rate, obtain a large amount of renewable energy while handling livestock and poultry sewage.
Description
Technical field
The present invention relates to sewage treatment field, the method for specifically a kind of bacteria-algae synergism efficient process aquaculture wastewater.
Background technique
It is traced for a long time using the history that microalgae carries out sewage treatment, early in the 1950s, Oswald etc. just proposes benefit
With the imagination of microalgae processing sewage, micro algae growth process needs the nutrients such as a large amount of absorbed nitrogens, phosphorus, can directly reduce by two/tri-
The content of the pollutants such as N, P in grade water outlet, can also by indirectly-actings, microalgaes such as fixed carbon dioxide, generation oxygen, raising pH
Create the environmental condition for effectively removing residual organic matter and pathogenic microbes in water.In addition, microalgae also has an absorption huge sum of money
The ability of the harmful substances such as category, therefore, microalgae have the good potentiality as deep purification of waste water technology.
In recent years, domestic and foreign scholars carry out in terms of exploitation microalgae deep purification of waste water and production of renewable energy resources potentiality
Numerous studies；In sewage purification mechanism, Strain selection, reactor design, process regulation and alga cells processing and utilization
Etc. all achieve positive progress.However microalgae cell is generally less than 30 microns, it is negatively charged, density close to water, these
Characteristic makes frustule in water often in stable suspended state, is difficult to precipitate by gravity like that activated sludge real
Existing natural separation.As a result, frustule can be largely lost with processing water, not only secondary pollution handles water, but also causes in reactor
Biomass is difficult to largely maintain (being typically only 0.2 ~ 0.6g/L).Low culture density causes removal efficiency low, so that processing
Effect stability is poor.Furthermore microalgae handles livestock and poultry sewage, not high to ammonia nitrogen tolerance level, influences to grow, leads to total nitrogen and total phosphorus
Removal efficiency is slack-off, and undesirable to COD removal effect.
Summary of the invention
One of the objects of the present invention is to provide a kind of advantages that can make full use of bacteria-algae synergism, and sewage treating efficiency is high,
Aeration uniformly, can accelerate microalgae photosynthesis, the dress of the bacteria-algae synergism efficient process aquaculture wastewater of mass propgation microalgae biomembrane
It sets.
The second object of the present invention is to act synergistically by microbial function bacterium and microalgae, quickly reduces in livestock and poultry sewage
The pollutants such as nitrogen, phosphorus, shorten hydraulic detention time, micro algae biomass accumulating rate improved, while handling livestock and poultry sewage
The method for obtaining the bacteria-algae synergism efficient process aquaculture wastewater of a large amount of renewable energy.
The present invention to achieve the above object, the technical solution taken are as follows:
The device of bacteria-algae synergism efficient process aquaculture wastewater, including compound bacteria aeration tank and gas-lifting type column bioreactor.
Compound bacteria aeration tank water inlet connects the water outlet of grid pond by water pipe, and compound bacteria aeration tank water outlet connects gas by water pipe
The water inlet of lift-type column bioreactor；Gas-lifting type column bioreactor has 1 ~ 10, connects in a series arrangement；Most
The top delivery port of the gas-lifting type column bioreactor of end is connected by water pipe with the water inlet of sedimentation basin；Sedimentation basin
Water outlet is connected by water pipe with the water inlet in ozone decolorization pond；The water inlet phase of the water outlet in ozone decolorization pond and discharge bay
Even；The water inlet of grid pond is connected with the water outlet of former pond.Autotrophic ammonia oxidizing bacterium, Autotrophic nitrification bacterium and allotrophic nitrobacteria association
Ammonia nitrogen in livestock and poultry sewage is first converted into nitrate nitrogen by same-action, is relieved the inhibition of microalgae, is provided needed for micro algae growth
Nitrogen source.Then the microalgae that deposit number is CCTCC NO:M 2017461 is closed the contaminant degradation in sewage by assimilation
At Self substances, can not only ammonia nitrogen, total nitrogen, nitrate nitrogen and total phosphorus in efficient degradation sewage, COD reduces obvious, also has life
Object flocculating function has suction-operated to suspended matter in water and total phosphorus, reduces water quality coloration.It is assisted by microbial function bacterium and microalgae
Same-action quickly reduces the pollutants such as nitrogen, the phosphorus in livestock and poultry sewage, shortens hydraulic detention time, improves micro algae biomass accumulation
Speed obtains a large amount of renewable energy while handling livestock and poultry sewage.
Membrane module is equipped in gas-lifting type column bioreactor；Gas-lifting type column bioreactor bottom is connected with exposure
Tracheae and bottom water outlet, aeration tube are connect with air blower, and pressure gauge is equipped between air blower and aeration tube；Bottom water outlet is set
There is valve and connect by water pipe with top water inlet, the water pipe of connection is equipped with circulating pump, and circulating pump water outlet is connected with pH
Meter；It is additionally provided with top delivery port at the top of gas-lifting type column bioreactor, water outlet is also connected with flowmeter.
Membrane module is polyvinylidene fluoride hollow fiber, and membrane module surface is in fish scale-shaped structure, and the membrane aperture of membrane module is
0.08 ~ 0.15 μm, film surface area is 0.08 ~ 0.15 ㎡.The fish scale-shaped structure energy effective protection fenestra on membrane module surface not by
Microalgae is completely plugged, guarantees preferable translucency, and the bubble that can also increase between biomembrane fenestra when aeration generates, increases
Absorption of the microalgae to the utilization rate of carbon dioxide, conducive to microalgae to carbon source.
Current divider is equipped with above aeration tube, current divider is made of gas collecting tube and shunting body, and gas collecting tube is connected with aeration tube,
Shunting body is set to above gas collecting tube, and shunting body is centrum, and inside is all provided with tap hole, and external helicoid is surrounded with helicla flute.It shunts
Gas is refined the bubble volume to form the gas produced than aeration tube through tap hole by the gas that device collects right above aeration tube
Smaller bubble reaches and cuts bubble effect, and bubbles volume is big and the aeration wider impact force of range is small, and the helicla flute outside current divider can make
Spirally mode rises the bubble that aeration tube generates, and reduces bubble to the impact force of microalgae, is conducive to microalgae in photoproduction
Microalgae position in object reactor is stablized, and effectively avoids microalgae from separating in aeration process with the membrane module of absorption, by exposing
Setting current divider solves that aeration intensity in the prior art is excessive to cause microalgae to be unevenly distributed and be aerated range mistake above tracheae
In the problems in collection.
Tap hole outlet end is connected with movable screen, and web plate surface is reflective surface, and gas passes through web plate in aeration process
When can produce the bubble of micro volume and slow down the speed that bubble rises in water, avoid the generation of air pocket, extend microalgae with
The time of contact of carbon dioxide in bubble further increases the generation of bubble in conjunction with membrane module, greatly improves carbon dioxide
Utilization rate promotes the micro algae growth on membrane module surface, and when a large amount of bubbles pass through web plate, web plate can generate bending, bending
The surface of web plate afterwards can expand the range of refractive for being irradiated to the light on current divider surface, make some light in bioreactor
Variation occurs for the moment, is conducive to improve microalgae photosynthesis, promotes the production of microalgae biomembrane.
It is arranged in a crossed manner in gas collecting tube to have turbulator rod, the turquoise piece of annular is evenly equipped on turbulator rod, flag and turbulator rod
Diameter ratio is 2 ~ 3:1, and turbulator rod arranged in a crossed manner can be changed the motion state of air-flow, hand over the gas of script vertical ascent
Fork rises, and annular turquoise piece is arranged and further increases flow-disturbing effect, so that gas streamline is changed and rapid dispersion and arrives
Up to tap hole bottom, divide tolerance uniform, realize aeration uniformly, improves the speed of production and production thickness of biomembrane, while green
Air pressure when turquoise piece and turbulator rod flow-disturbing in gas collecting tube generates nitrogen, oxygen, carbon dioxide and rare gas in variation air
Composition ratio, increase the proportion of Carbon Dioxide in Air, be beneficial to improve the photosynthesis of microalgae and biomembrane is raw
It produces, passes through formula, Q was the total flow of gas collecting tube,For discharge coefficient, AeFor gas collecting tube hole face
Product,For gas flow rate, g is chaff interferent diameter, Pa is atmospheric pressure, and P1 is gas collection intraductal pressure, preferably flag and turbulator rod
Diameter ratio be 2-3:1, rapid dispersion and reach tap hole bottom to guarantee that gas streamline caused by flow-disturbing changes, point
Tolerance is uniform, realizes aeration uniformly, improves the speed of production of biomembrane and produces the effect of thickness.
Grid is equipped in grid pond；It is taper below sedimentation basin, and is equipped with sewage draining exit, adds flocculation in such a way that stream adds
Agent；Ozone decolorization bottom of pond portion is equipped with ozone reaction device；The water pipe that grid pond is connect with compound bacteria aeration tank is equipped with check valve.
Before livestock and poultry sewage enters compound bacteria aeration tank, grid pond carries out it to make preliminary purification, removes bulky grain suspended matter；Through microalgae
Contain a certain amount of microalgae suspended matter in the water of biological treatment, these microalgae suspended matter flocculation sedimentations are improved water quality by sedimentation basin；
The synergistic effect of microbial function bacterium and microalgae is fabulous but unsatisfactory to the removal of pigment to the nitrogen phosphorus ligands effect in water,
So the final step setting ozone decolorization pond of the water purification needed is decolourized and is sterilized to processing water.
The method of bacteria-algae synergism efficient process aquaculture wastewater, bacterium algae compounding combination processing livestock and poultry sewage, specifically first uses
Ammonia nitrogen in livestock and poultry sewage is converted into nitrate nitrogen by autotrophic ammonia oxidizing bacterium, Autotrophic nitrification bacterium and allotrophic nitrobacteria synergistic effect,
Then by treated, sewage is passed through gas-lifting type column bioreactor, is CCTCC NO:M's 2017461 using deposit number
The dephosphorization of microalgae denitrification and the accumulation for carrying out biomass.The microalgae classification naming that deposit number is CCTCC NO:M 2017461 is bead
Algae CS-3(Chlorella sorokiniana strain CS-3) on August 31st, 2017 it was preserved in Chinese Typical Representative culture
Collection (CCTCC), the address of above-mentioned collection are Wuhan, China university.The above method goes to denitrogenate the high-efficient of phosphorus, stops
It stays the time ten days or so, ammonia nitrogen removal frank is 96% or more, nitrite nitrogen does not accumulate, nitrogen removal rate is 85% or more, total phosphorus
95% or more removal rate and COD removal rate are 90% or more.
Microalgae 16srDNA sequence are as follows:
AGGTCTGTTAGATTAGCCATGCATGTCTAAGTATAAACTGCTTTATACTGTGAAACTGCGAATGGCTCATTAA
ATCAGTTATAGTTTATTTGATGGTACCTACTACTCGGATACCCGTAGTAAATCTAGAGCTAATACGTGCGTAAATCC
CGACTTCTGGAAGGGACGTATTTATTAGATAAAAGACCGACCGGGCTTGCCCGACTCGCGGTGAATCATGATAACTT
CACGAATCGCATGGCCTCGTGCCGGCGATGTTTCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGAGG
CCTACCATGGTGGTAACGGGTGACGGAGGATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATC
CAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACAGGGAGGTAGTGACAATAAATAACAATACTGGGCCT
TTTCAGGTCTGGTAATTGGAATGAGTACAATCTAAACCCCTTAACGAGGATCAATTGGAGGGCAAGTCTGGTGCCAG
CAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTTAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATTTCGGGTG
GGGCCTGCCGGTCCGCCGTTTCGGTGTGCACTGGCAGGGCCCACCTTGTTGCCGGGGACGGGCTCCTGGGCTTCACT
GTCCGGGACTCGGAGTCGGCGCTGTTACTTTGAGTAAATTAGAGTGTTCAAAGCAGGCCTACGCTCTGAATACATTA
GCATGGAATAACACGATAGGACTCTGGCCTATCCTGTTGGTCTGTAGGACCGGAGTAATGATTAAGAGGGACAGTCG
GGGGCATTCGTATTTCATTGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGAACTACTGCGAAAGCATTTGCCAA
GGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATTAGATACCGTCCTAGTCTCAACCATAAACG
ATGCCGACTAGGGATCGGCGGATGTTTCTTCGATGACTCCGCCGGCACCTTATGAGAAATCAAAGTTTTTGGGTTCC
GGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGCGTGGAGCCTGCGGCTTA
ATTTGACTCAACACGGGAAAACTTACCAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCT
ATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGGTTGCCTTGTCAGGTTGATTCCGGTAACGAACGAGACCTCAG
CCTGCTAAATAGTCACGGTTGGCTCGCCAGCCGGCGGACTTCTTAGAGGGACTATTGGCGACTAGCCAATGGAAGCA
TGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGCATTCAACGAGCCT
AGCCTTGGCCGAGAGGCCCGGGTAATCTTTGAAACTGCATCGTGATGGGGATAGATTATTGCAATTATTAATCTTCA
ACGAGGAATGCCTAGTAAGCGCAAGTCATCAGCTTGCGTTGATTACGTCCCTGCCCTTTGTACACACCGCCCGTCGC
TCCTACCGATTGGGTGTGCTGGTGAAGTGTTCGGATTGGCGACCGGGGGCGGTCTCCGCTCTCGGCCGCCGAGAAGT
TCATTAAACCCTCCCACCTAGAGAAGAGAAGTCGAGATTCCCAC。
Compared with the prior art, the advantages of the present invention are as follows: it is acted synergistically by microbial function bacterium and microalgae, fast prompt drop
The pollutants such as nitrogen, phosphorus in low livestock and poultry sewage shorten hydraulic detention time, improve micro algae biomass accumulating rate, raise in processing
A large amount of renewable energy are obtained while fowl sewage.New gas-lifting type column bioreactor structure is devised, film group is passed through
The fish scale-shaped structure energy effective protection fenestra on part surface is not completely plugged by microalgae, guarantees preferable translucency, can also be aerated
When the bubble that increases between biomembrane fenestra generate, increase microalgae to the utilization rate of carbon dioxide, conducive to microalgae to carbon source
It absorbs；Current divider is set above aeration tube and solves that aeration intensity in the prior art is excessive to cause microalgae to be unevenly distributed and expose
Gas range the problems in excessively collects；Mass propgation microalgae biomembrane to the nitrogen phosphorus ligands in water body, realizes microalgae immobilization simultaneously
Culture is conducive to the operation such as subsequent algae solution dehydration, desiccation.
Detailed description of the invention
Fig. 1 is O-GTCP of the present invention (aerobic tank-gas-lifting type column Photoreactor) process flow chart；
Fig. 2 is the schematic diagram of gas-lifting type column Photoreactor of the present invention；
Fig. 3 is current divider of the present invention and aeration tube mated condition schematic diagram；
Fig. 4 is the cross-sectional view under current divider of the present invention and aeration tube mated condition；
Fig. 5 is web plate schematic diagram of the invention.
Description of symbols: 1 former pond；2 grid ponds；3 compound bacteria aeration tanks；4 gas-lifting type column bioreactors；5
Sedimentation basin；6 ozone decolorization ponds；7 discharge bays；8 grids；9 check valves；10 ozone reaction devices；11 sewage draining exits；12 current dividers；12a
Gas collecting tube；12b shunting body；12c tap hole；12d web plate；12e helicla flute；12f turbulator rod；13 aeration tubes；14 air blowers；15 pressures
Power table；17 membrane modules；18 flowmeters；19 top delivery ports；20pH meter；21 circulating pumps；22 water inlets.
Specific embodiment
The present invention program is described further below by drawings and examples:
Embodiment 1:
As shown in Figure 1, the device of bacteria-algae synergism efficient process aquaculture wastewater, including compound bacteria aeration tank 3 and gas-lifting type column light
Bioreactor 4.3 water inlet of compound bacteria aeration tank connects the water outlet of grid pond 2 by water pipe, and compound bacteria aeration tank 3 is discharged
Mouth connects the water inlet of gas-lifting type column bioreactor 4 by water pipe；Gas-lifting type column bioreactor 4 has 3, with
Series system connection；The water outlet of the gas-lifting type column bioreactor 4 of least significant end passes through the water inlet of water pipe and sedimentation basin 5
It is connected；The water outlet of sedimentation basin 5 is connected by water pipe with the water inlet in ozone decolorization pond 6；The water outlet in ozone decolorization pond 6 with go out
The water inlet in pond 7 is connected；The water inlet of grid pond 2 is connected with the water outlet of former pond 1.Grid 8 is equipped in grid pond 2；It is heavy
It is taper below shallow lake pond 5, and is equipped with sewage draining exit 11, adds flocculant in such a way that stream adds；6 bottom of ozone decolorization pond is equipped with smelly
Oxygen reaction unit 10；The water pipe that grid pond 2 is connect with compound bacteria aeration tank 3 is equipped with check valve 9.
As shown in Fig. 2, being equipped with membrane module 17 in gas-lifting type column bioreactor 4；Gas-lifting type column optical-biological reaction
4 bottom of device is connected with aeration tube 13 and bottom water outlet, and aeration tube 13 is connect with air blower 14, air blower 14 and aeration tube 13 it
Between be equipped with pressure gauge 15；Bottom water outlet is equipped with valve and is connect by water pipe with top water inlet, and the water pipe of connection is equipped with
Circulating pump 21,21 water outlet of circulating pump are connected with pH meter 20；Top water outlet is additionally provided at the top of gas-lifting type column bioreactor 4
Mouth 19, top delivery port 19 is also connected with flowmeter 18.
Membrane module 17 is polyvinylidene fluoride hollow fiber, and 17 surface of membrane module is in fish scale-shaped structure, the membrane aperture of membrane module
It is 0.1 μm, film surface area is 0.1 ㎡.
As shown in Fig. 3 ~ 5, current divider 12 is equipped with above aeration tube 13, current divider 12 is by gas collecting tube 12a and shunting body 12b group
At gas collecting tube 12a is connected with aeration tube 13, and shunting body 12b is set to above gas collecting tube 12b, and shunting body 12b is centrum, interior
Portion is all provided with tap hole 12c, and external helicoid is surrounded with helicla flute 12e.The outlet end tap hole 12c is connected with movable screen 12d, web plate
The surface 12d is reflective surface.It is arranged in a crossed manner in gas collecting tube 12a to have turbulator rod 12f, the turquoise of annular is evenly equipped on turbulator rod 12f
The diameter ratio of piece, flag and turbulator rod 12f are 2.6:1.
Embodiment 2:
As shown in Fig. 1 ~ 5, the method for bacteria-algae synergism efficient process aquaculture wastewater: livestock and poultry sewage flows into grid pond 2 from former pond 1,
Grid pond 2 carries out it to make preliminary purification, removes bulky grain suspended matter.Then compound bacteria aeration tank 3 is flowed into, it is autotrophic ammonia oxidizing
Ammonia nitrogen in livestock and poultry sewage is converted into nitrate nitrogen by bacterium, Autotrophic nitrification bacterium and allotrophic nitrobacteria synergistic effect.By treated
Sewage is passed through gas-lifting type column bioreactor 4 from water inlet 22, is the micro- of CCTCC NO:M 2017461 using deposit number
The dephosphorization of algae denitrification and the accumulation for carrying out biomass.Membrane module 17 built in gas-lifting type column bioreactor 4 adheres to as microalgae
Material, 4 bottom of gas-lifting type column bioreactor aeration, aeration intensity 1.5L/min utilize lamp or artificial light
Source guarantees microalgae photosynthesis in gas-lifting type column bioreactor 4, and microdisk electrode is in the process by livestock and poultry sewage by gas-lifting type
Column bioreactor 4 is sent into from water inlet 22, and the sewage of feeding is discharged from bottom water outlet, using circulating pump 21 by livestock and poultry
Sewage is re-fed into gas-lifting type column bioreactor 4 from top water inlet, forms circulation material conveying.Made using membrane module 17 micro-
Frustule is adsorbed on 17 surface of membrane module and forms microalgae biomembrane, it can be achieved that microalgae Immobilized culture, it is de- to be conducive to subsequent algae solution
The operation such as water and desiccation.Pressure gauge 15 monitors aeration intensity, prevents excess aeration dissolved oxygen amount excessively high, avoids the excessive influence of aeration quantity
The waste of microdisk electrode and aeration energy consumption.The water handled through several gas-lifting type column bioreactors 4 flows into sedimentation basin 5.
Discharge bay 7 is flowed into after carrying out decoloration disinfection to water by sedimentation basin 5 and ozone decolorization pond 6 again.The above method goes to denitrogenate the effect of phosphorus
Rate is high, the residence time ten days or so, ammonia nitrogen removal frank is 96% or more, nitrite nitrogen does not accumulate, nitrogen removal rate be 85% with
Upper, total 95% or more tp removal rate and COD removal rate are 90% or more.
Embodiment 3:
Biomass estimation: surveying the OD value of microalgae liquid in embodiment 1, while microscopic count at the 680nm, the microalgae less than 10 μm,
It is counted with blood counting chamber, the 0.1mL phytoplankton counting frame of the algae greater than 10 μm counts cell density (at least counting 3 times).
It is mapped with OD value to cell density, seeks its absorptivity.Then formula (lg(m)=- 1.0465ln(x is utilized)+4.255, wherein
M is biomass, and x is absorptivity) biomass is converted by absorptivity.Embodiment 1 after measurement processing 15d according to the method described above
The accumulation of biomass in microalgae.
Crude protein content measurement: contained using crude protein in microalgae in embodiment 1 after micro triumphant formula nitriding measurement processing 15d
Amount.
Fat content measurement: using fat content in microalgae in embodiment 1 after Sudan black B Determination Staining processing 15d.
Measurement result is as shown in table 1.
Ammonia nitrogen, total nitrogen, nitrate nitrogen, total phosphorus, COD assay: referring to national standard, " note is flowed in the measurement of Water quality ammonia nitrogen
Penetrate-salicylic acid spectrophotometry " (HJ 666-2013), the front and back livestock and poultry sewage treatment 15d ammonia-nitrogen content in embodiment 1 is measured,
Calculate removal rate；Referring to national standard " measurement flow injection-hydrochloride naphthodiamide spectrophotometry of water quality total nitrogen " (HJ
668-2013), the front and back livestock and poultry sewage treatment 15d total nitrogen content in embodiment 1 is measured, removal rate is calculated；Referring to national standard " water
The measurement spectroscopic assay of matter nitrite nitrogen and nitrate nitrogen and passing flux analysis (CFA and FIA) " (NF T90-012-1996
(R2002)) front and back livestock and poultry sewage treatment 15d nitrate nitrogen content in embodiment 1, is measured, removal rate is calculated；Referring to national standard
" the measurement ammonium molybdate spectrophotometric method of water quality total phosphorus " (GB/T 11893-1989) measures livestock and poultry sewage treatment in embodiment 1
Total phosphorus content before and after 15d calculates removal rate；Referring to national standard " COD (COD) analyzer " (GB/T 32208-
2015), the front and back livestock and poultry sewage treatment 15d COD value in measurement embodiment 1, calculates removal rate.Measurement result is as shown in table 2.
Colority of Sewage Water measurement: referring to national standard " measurement of water quality coloration " (GB/T 11903-1989), measurement is implemented
Light absorption value at the 450nm of the front and back livestock and poultry sewage treatment 15d in example 1.Measurement result is as shown in table 3.
1 micro algae biomass of table, crude protein and fat content
| Project | Biomass | Biomass crude protein | Unit grease |
| Content | 1.8g/L | 35% | 37% |
2 ammonia nitrogen of table, total nitrogen, nitrate nitrogen, total phosphorus, COD removal rate
| Project | Ammonia nitrogen | Total nitrogen | Nitrate nitrogen | Total phosphorus | COD |
| Removal rate | 97.42% | 93.28% | 99.32% | 95.78% | 93.59% |
The variation of 3 450nm light absorption value of table
| Before processing | After processing | |
| Light absorption value | 0.352 | 0.068 |
As shown in Table 1, the method for the present invention handles livestock and poultry sewage, and microalgae photosynthetic efficiency is high, will be in sewage by assimilation
Contaminant degradation synthesize Self substances, fast culture obtains microalgae biomembrane, is used to prepare biological bavin as renewable energy
Oil prepares the fields such as albumen.As shown in Table 2, the method for the present invention goes to denitrogenate the high-efficient of phosphorus, residence time 15d, ammonia nitrogen removal
Rate is 96% or more, nitrite nitrogen does not accumulate, nitrogen removal rate is 85% or more, 95% or more total tp removal rate and COD removal rate
It is 90% or more.The method of the present invention is good to the decolorization flocculation effect of livestock and poultry sewage as shown in Table 3.In conclusion the present invention handles poultry
Fowl sewage obtains a large amount of renewable energy while handling livestock and poultry sewage, and no matter the accumulating rate or nitrogen of micro algae biomass
Tp removal rate is all better than the prior art, has significant progress.
Routine operation in operating procedure of the invention is well known to those skilled in the art, herein without repeating.
Technical solution of the present invention is described in detail in embodiment described above, it should be understood that the above is only
For specific embodiments of the present invention, it is not intended to restrict the invention, all any modifications made in spirit of the invention,
Supplement or similar fashion substitution etc., should all be included in the protection scope of the present invention.
Sequence table
<110>Zhejiang Academy of Agricultural Science
<120>method of bacteria-algae synergism efficient process aquaculture wastewater
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1734
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 1
aggtctgtta gattagccat gcatgtctaa gtataaactg ctttatactg tgaaactgcg 60
aatggctcat taaatcagtt atagtttatt tgatggtacc tactactcgg atacccgtag 120
taaatctaga gctaatacgt gcgtaaatcc cgacttctgg aagggacgta tttattagat 180
aaaagaccga ccgggcttgc ccgactcgcg gtgaatcatg ataacttcac gaatcgcatg 240
gcctcgtgcc ggcgatgttt cattcaaatt tctgccctat caactttcga tggtaggata 300
gaggcctacc atggtggtaa cgggtgacgg aggattaggg ttcgattccg gagagggagc 360
ctgagaaacg gctaccacat ccaaggaagg cagcaggcgc gcaaattacc caatcctgac 420
acagggaggt agtgacaata aataacaata ctgggccttt tcaggtctgg taattggaat 480
gagtacaatc taaacccctt aacgaggatc aattggaggg caagtctggt gccagcagcc 540
gcggtaattc cagctccaat agcgtatatt taagttgctg cagttaaaaa gctcgtagtt 600
ggatttcggg tggggcctgc cggtccgccg tttcggtgtg cactggcagg gcccaccttg 660
ttgccgggga cgggctcctg ggcttcactg tccgggactc ggagtcggcg ctgttacttt 720
gagtaaatta gagtgttcaa agcaggccta cgctctgaat acattagcat ggaataacac 780
gataggactc tggcctatcc tgttggtctg taggaccgga gtaatgatta agagggacag 840
tcgggggcat tcgtatttca ttgtcagagg tgaaattctt ggatttatga aagacgaact 900
actgcgaaag catttgccaa ggatgttttc attaatcaag aacgaaagtt gggggctcga 960
agacgattag ataccgtcct agtctcaacc ataaacgatg ccgactaggg atcggcggat 1020
gtttcttcga tgactccgcc ggcaccttat gagaaatcaa agtttttggg ttccgggggg 1080
agtatggtcg caaggctgaa acttaaagga attgacggaa gggcaccacc aggcgtggag 1140
cctgcggctt aatttgactc aacacgggaa aacttaccag gtccagacat agtgaggatt 1200
gacagattga gagctctttc ttgattctat gggtggtggt gcatggccgt tcttagttgg 1260
tgggttgcct tgtcaggttg attccggtaa cgaacgagac ctcagcctgc taaatagtca 1320
cggttggctc gccagccggc ggacttctta gagggactat tggcgactag ccaatggaag 1380
catgaggcaa taacaggtct gtgatgccct tagatgttct gggccgcacg cgcgctacac 1440
tgatgcattc aacgagccta gccttggccg agaggcccgg gtaatctttg aaactgcatc 1500
gtgatgggga tagattattg caattattaa tcttcaacga ggaatgccta gtaagcgcaa 1560
gtcatcagct tgcgttgatt acgtccctgc cctttgtaca caccgcccgt cgctcctacc 1620
gattgggtgt gctggtgaag tgttcggatt ggcgaccggg ggcggtctcc gctctcggcc 1680
gccgagaagt tcattaaacc ctcccaccta gagaagagaa gtcgagattc ccac 1734
Claims (8)
1. the device of bacteria-algae synergism efficient process aquaculture wastewater, including compound bacteria aeration tank (3) and gas-lifting type column photo-biological it is anti-
Answer device (4), it is characterised in that: water outlet of compound bacteria aeration tank (3) water inlet by water pipe connection grid pond (2), institute
It states compound bacteria aeration tank (3) water outlet and passes through the water inlet (22) of water pipe connection gas-lifting type column bioreactor (4)；It is described
Gas-lifting type column bioreactor (4) has 1 ~ 10, connects in a series arrangement；The gas-lifting type column photo-biological of the least significant end
The top delivery port (19) of reactor (4) is connected by water pipe with the water inlet of sedimentation basin (5)；The water outlet of the sedimentation basin (5)
Mouth is connected by water pipe with the water inlet of ozone decolorization pond (6)；The water outlet of the ozone decolorization pond (6) and discharge bay (7)
Water inlet is connected；The water inlet of the grid pond (2) is connected with the water outlet of former pond (1).
2. the device of bacteria-algae synergism efficient process aquaculture wastewater according to claim 1, it is characterised in that: the gas-lifting type
Membrane module (17) are equipped in column bioreactor (4)；Gas-lifting type column bioreactor (4) bottom is connected with exposure
Tracheae (13) and bottom water outlet, aeration tube (13) are connect with air blower (14), the air blower (14) and aeration tube (13) it
Between be equipped with pressure gauge (15)；The bottom water outlet is equipped with valve and is connect by water pipe with top water inlet, the water pipe of connection
It is equipped with circulating pump (21), circulating pump (21) water outlet is connected with pH meter (20)；The gas-lifting type column bioreactor (4)
Bottom is equipped with water inlet (22), and top is additionally provided with top delivery port (19), and the top delivery port (19) is also connected with flowmeter
(18).
3. the device of bacteria-algae synergism efficient process aquaculture wastewater according to claim 2, it is characterised in that: the membrane module
It (17) is polyvinylidene fluoride hollow fiber, membrane module (17) surface is in fish scale-shaped structure, and the membrane aperture of membrane module is 0.08 ~
0.15 μm, film surface area is 0.08 ~ 0.15 ㎡.
4. the device of bacteria-algae synergism efficient process aquaculture wastewater according to claim 2, it is characterised in that: the aeration tube
(13) top is equipped with current divider (12), and the current divider (12) is made of gas collecting tube (12a) and shunting body (12b), gas collecting tube
(12a) is connected with aeration tube (13), and shunting body (12b) is set to above gas collecting tube (12a), and shunting body (12b) is centrum, interior
Portion is all provided with tap hole (12c), and external helicoid is surrounded with helicla flute (12e).
5. the device of bacteria-algae synergism efficient process aquaculture wastewater according to claim 4, it is characterised in that: the tap hole
The outlet end (12c) is connected with movable screen (12d), and the web plate surface (12d) is reflective surface.
6. the device of bacteria-algae synergism efficient process aquaculture wastewater according to claim 4, it is characterised in that: the gas collecting tube
It is arranged in a crossed manner in (12a) to have turbulator rod (12f), the turquoise piece of annular, flag and flow-disturbing are evenly equipped on the turbulator rod (12f)
The diameter ratio of bar (12f) is 2 ~ 3:1.
7. the device of bacteria-algae synergism efficient process aquaculture wastewater according to claim 1, it is characterised in that: the grid pond
(2) grid (8) are equipped in；It is taper below the sedimentation basin (5), and is equipped with sewage draining exit (11), is added in such a way that stream adds
Flocculant；Ozone decolorization pond (6) bottom is equipped with ozone reaction device (10)；The grid pond (2) and compound bacteria aeration tank
(3) water pipe connected is equipped with check valve (9).
8. the method for bacteria-algae synergism efficient process aquaculture wastewater, it is characterised in that bacterium algae compounding combination processing livestock and poultry sewage, specifically
It is first to be converted into the ammonia nitrogen in livestock and poultry sewage using autotrophic ammonia oxidizing bacterium, Autotrophic nitrification bacterium and allotrophic nitrobacteria synergistic effect
Nitrate nitrogen, then by treated, sewage is passed through gas-lifting type column bioreactor (4), is CCTCC NO:M using deposit number
2017461 microalgae denitrification dephosphorization and the accumulation for carrying out biomass.
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| CN201810048400.8A CN110156242B (en) | 2018-01-18 | 2018-01-18 | Method for efficiently treating aquaculture sewage through cooperation of bacteria and algae |
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| CN201810048400.8A CN110156242B (en) | 2018-01-18 | 2018-01-18 | Method for efficiently treating aquaculture sewage through cooperation of bacteria and algae |
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| CN115784431A (en) * | 2022-12-07 | 2023-03-14 | 哈尔滨工业大学 | Membrane for supplying CO for maintaining carbon-oxygen balance of optical biological membrane system 2 Sewage treatment method and device |
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