CN103121773A - Biological denitrification method of low-COD (chemical oxygen demand) high-ammonia-nitrogen municipal sewage with microbes - Google Patents
Biological denitrification method of low-COD (chemical oxygen demand) high-ammonia-nitrogen municipal sewage with microbes Download PDFInfo
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Abstract
The invention relates to a biological denitrification method of low-COD (chemical oxygen demand) high-ammonia-nitrogen municipal sewage with microbes, which is characterized by comprising steps as follows: (1) a high-efficiency nitrifying bacterium inoculum is prepared; (2) a high-efficiency denitrifying bacterium inoculum is prepared; (3) low-COD high-ammonia-nitrogen sewage and domestic wastewater is delivered into an anoxic tank filled with the high-efficiency denitrifying bacterium inoculum, and stays for 6-24 hours; (4) the sewage subjected to anoxic reaction is delivered into an aerobic tank filled with the high-efficiency nitrifying bacterium inoculum, and stays for 2-24 hours, wherein the volume ratio of the aeration amount to the water inlet amount of the aerobic tank is (15-25):1; (6) the sewage subjected to aerobic reaction flows back to the anoxic tank to react with the denitrifying bacterium inoculum; (7) the sewage enters the aerobic tank again to be subjected to biological denitrification treatment; and (8) the sewage subjected to secondary aerobic treatment passes through a hollow fiber microfiltration membrane and is continuously discharged. The invention has the advantages of low cost, no toxicity, no pollution, high efficiency and the like, and is easy to implement.
Description
Technical field
The invention belongs to the improvement technical field of municipal effluent, especially a kind ofly utilize microorganism to carry out biological denitrification method to the low high ammonia nitrogen municipal effluent of COD.
Background technology
Along with the fast development of China's economy and the raising day by day of living standards of the people, urban wastewater discharge significantly increases.The expansion of the discharging of a large amount of percolates, movement, bath water and suitability for industrialized production scale, directly cause the significantly raising of the pollutant load such as nitrogen phosphate and sulfur in municipal effluent, because most of organism easily is biodegradable, and organic nitrogen compound is converted into ammonia nitrogen through ammonification, make ammonia nitrogen in sewage concentration raise, directly cause the C/N of sewage on the low side.Adopt conventional biological denitrification method and technique, in sewage, the clearance of total nitrogen is lower.
in recent years, country is to environment protection, the dynamics of environmental improvement is progressively strengthened, 2000 No. 36 literary compositions of State Council just once proposed, whole treatment rates of domestic sewage will reach 60% by 2010, the concept of " reusing sewage " after also having proposed simultaneously to process, this just has higher requirement to the emission standard of municipal effluent, 2002 on the basis that relevant department investigates further, country has promulgated " urban wastewater treatment firm pollutant emission standard " again, and some pollutant emission of the sewage work in a line city must reach the one-level A standard in this standard, must reach below 5mg/L when wherein the ammonia nitrogen index request in sewage discharges, this just has higher requirement to the denitride technology of urban wastewater treatment firm.
At present, China generally is divided into A/O method, A2/O method, oxidation ditch process and the large class of SBR method four for the municipal effluent biological denitrificaion treatment process.
A/O denitrifying activated sludge method: A/O technique is the most basic nitrated, denitrification denitrogenation technique.This technique does not need the additional carbon denitrogenation, is easy to control sludge bulking, and investment and working cost are lower, widespread use in wastewater treatment in China factory.
A2/O denitrogenation dephosphorizing activated sludge process: A2/O(A/A/O) method is a kind of of anaerobism-anoxic-aerobic biological denitrification dephosphorization process, but this technique is only in the situation that water inlet C/N and C/P is higher could realize synchronous denitrification dephosphorizing, therefore, be subject to certain condition restriction on using.
Oxidation ditch process: oxidation ditch has another name called continuous circulation aeration tank (Continuous Loop Reactor), is a kind of distortion of activated sludge process.But oxidation ditch does not belong to special biological dephosphorize denitrification technique, need to combine with other technique just to have good Nitrogen removal effect.
SBR technique: SBR is sequencing batch reactor, is a kind of active sludge sewage disposal technology that moves by the intermittent aeration mode, claims again sequencing batch active sludge.But this technique effect aspect denitrogenation dephosphorizing is not very stable.
Increasingly strict along with China's water quality environment standard, the especially raising of ammonia nitrogen and total nitrogen emission standard, after the traditional biological denitrogenation method can't make and process, the ammonia nitrogen index of municipal effluent reaches country-level A emission standard.Newly-built municipal sewage plant must consider the removal of nitrogen pollutant, also will carry out the transformation of denitrogenation dephosphorizing to completed sewage work by stages.
Efficient dominant bacteria technology adopts the modern biotechnology means to screen, tame for difficult degradation in low c/N sewage, virulent pollution substance (comprising total nitrogen, ammonia nitrogen) exactly, turns out strong adaptability, special bacterial classification that degradation rate is high.Form the symbiosis flora by compounded technology, optimize and the reinforced bioactivity sludge system.The method has the characteristics such as system stability is good, capacity of resisting impact load is strong.High efficiency composition microbial inoculum intensive treatment technology is at the early-stage in China, and the foreign technology use cost is high, and domestic enterprise can't accept substantially.Therefore the high efficiency composition microbial inoculum that research and development, production and use have independent intellectual property right becomes the task of top priority.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, provide that a kind of cost is low, nontoxicity, pollution-free, efficient is high, the microorganism that utilizes of easy realization carries out biological denitrification method to the low high ammonia nitrogen municipal effluent of COD.
The present invention solves its technical problem and takes following technical scheme to realize:
A kind ofly utilize microorganism to carry out biological denitrification method to the low high ammonia nitrogen municipal effluent of COD, it is characterized in that: steps of the method are:
⑴, preparation high efficiency nitrification microbial inoculum: the component of high efficiency nitrification microbial inoculum and compound proportion be:
With micrococcus sp A3 1-6
Corynebacterium A5 1-4
Corynebacterium A11 2-5,
High efficiency nitrification microbial inoculum pH scope is at 4.0-8.0, and bacterial concentration is 10
6-10
9Individual/ml;
⑵, the efficient denitrification microbial inoculum of preparation: efficiently the component of denitrification microbial inoculum and compound proportion is:
Rhodopseudomonas A6 1-6
Brevibacterium sp N7 1-8
Brevibacterium sp N3 1-5,
Efficient denitrification microbial inoculum pH scope is at 4.0-8.0, and bacterial concentration is 10
6-10
9Individual/ml;
⑶, that efficient denitrification microbial inoculum is fed into anoxic pond is standby, and the dosage first of efficient denitrification microbial inoculum is the 0.5-20% of anoxic pond useful volume;
⑷, will hang down COD high-ammonia-nitrogen sewage sanitary wastewater and be delivered to anoxic pond, stop 6-24 hour, control pH5.0-9.0, control temperature: 20-40 ℃, carry out the acidication processing of sewage;
⑸, the sewage transport after hypoxia response stopped 2-24 hour to the Aerobic Pond that has added described high efficiency nitrification microbial inoculum,
The dosage first of high efficiency nitrification microbial inoculum is the 0.5-20% of Aerobic Pond useful volume, controls pH5.0-9.0, controls temperature: 20-40 ℃, and Aerobic Pond aeration rate and flooding quantity volume ratio are 15-25: 1, carry out nitrification;
⑹ the sewage backflow after, aerobic reaction, carries out denitrification and processes with the reaction of denitrification microbial inoculum to anoxic pond;
⑺, sewage enter Aerobic Pond again, carry out the nitrogen stripping, realize the biological denitrificaion processing;
⑻ the sewage continuous blow-down after the hollow fiber microfiltration membrane assembly after, secondary aerobic treatment.
And described hollow fiber microfiltration membrane assembly adopts curtain formula hollow fiber microfiltration membrane, and material is anti-pollution polyvinylidene fluoride, and membrane pore size is 0.1-0.2 μ m.
And described low COD high-ammonia-nitrogen sewage sanitary wastewater is COD:1-100mg/l, NH
3The sanitary wastewater of-N water: 1-200mg/l.
Advantage of the present invention and positively effect are:
1, efficient advantage composite fungus agent used in the present invention can carry out denitrogenation processing in low C/N municipal effluent, and denitrification percent reaches 70%, solves that the denitrification effect that at present low C/N municipal sewage treatment faces is poor, the difficult problems such as processing and operation cost height.Application by this technology makes municipal effluent ammonia nitrogen and total nitrogen index reach country-level A effluent standard.
2. the present invention is on the basis of efficient dominant bacteria, set up a cover utilize MBR technique over the ground the C/N municipal effluent carry out the complete process route of biological denitrificaion, hollow fiber film assembly is adopted in design, solved the low problem of degradation efficiency that runs off and cause because of thalline in Black Liquor with Efficient Bacteria biochemical treatment process, make the cell concentration of advantage microbial inoculum remain optimum concn, thereby make the degradation effect to low C/N reach best.
3. be than the obvious mechanism of traditional biological method denitrification effect in the present invention: efficient advantage microbial inoculum mentioned in the present invention can carry out biological denitrificaion under the condition of water quality of low COD, and in the high efficiency nitrification microbial inoculum, the nitrite bacteria ratio is higher, and utilized MBR technique, make that in Aerobic Pond, bacteria concentration remains at high-level state, this just make high ammonia-nitrogen wastewater in water quality after the nitrification stage processing nitrogen mainly with NO2
-Exist, thereby saved time and the energy consumption of denitrification.
4. the present invention a kind ofly utilizes microorganism to carry out biological denitrification method to the low high ammonia nitrogen municipal effluent of COD, has the advantages such as cost is low, nontoxicity, pollution-free, efficient is high, easy realization.
Description of drawings
Fig. 1 is schematic flow sheet of the present invention.
Embodiment
Be further described below in conjunction with embodiment, following examples are descriptive, are not determinate, can not limit protection scope of the present invention with this.
Embodiment 1:
A kind ofly utilize microorganism to carry out biological denitrification method to the low high ammonia nitrogen municipal effluent of COD, steps of the method are:
⑴, preparation high efficiency nitrification microbial inoculum:
The component of high efficiency nitrification microbial inoculum is:
Be 500ml with micrococcus sp A3, Corynebacterium A5 is 300ml, and Corynebacterium A11 is that 200ml is mixed and made into the high efficiency nitrification microbial inoculum, and high efficiency nitrification microbial inoculum pH is 6.5, and bacterial concentration is 10
8Individual/ml;
⑵, the efficient denitrification microbial inoculum of preparation: efficiently the component of denitrification microbial inoculum and compound proportion is:
Rhodopseudomonas A6 is 200ml; Brevibacterium sp N7 is 500ml,, brevibacterium sp N3 is that 300ml is mixed and made into efficient denitrification microbial inoculum, and efficient denitrification microbial inoculum pH is 7.2, and bacterial concentration is 10
9Individual/ml;
⑶, to select useful volume be that anoxic pond and the useful volume of 50L is the Aerobic Pond of 50L.
Select and be COD:54mg/l, NH
3The sanitary wastewater 200Kg of-N water: 124mg/l.
With above-mentioned low COD high-ammonia-nitrogen sewage sanitary wastewater, be delivered to the anoxic pond that has added described efficient denitrification microbial inoculum, stopped 6 hours, the dosage first of efficient denitrification microbial inoculum is 2% of anoxic pond useful volume, be 1000ml, control pH6.0, control temperature: 30 ℃;
⑷, the sewage transport after hypoxia response are to the Aerobic Pond that has added described high efficiency nitrification microbial inoculum, stopped 4 hours, the dosage first of high efficiency nitrification microbial inoculum is that 2% of Aerobic Pond useful volume is 1000ml, control pH7.5, control temperature: 30 ℃, Aerobic Pond aeration rate and flooding quantity volume ratio are 20: 1;
⑸ the sewage backflow after, aerobic reaction, carries out denitrification and processes with the reaction of denitrification microbial inoculum to anoxic pond.
⑹, sewage enter Aerobic Pond again, carry out the nitrogen stripping, thereby realize the biological denitrificaion processing.
⑺ the continuous blow-down after the hollow fiber microfiltration membrane assembly of the sewage after, aerobic treatment.The hollow fiber microfiltration membrane assembly adopts curtain formula hollow fiber microfiltration membrane, and material is anti-pollution polyvinylidene fluoride, and membrane pore size is 0.1 μ m.
Aerobic Pond water outlet ammonia nitrogen concentration is monitored, do blank with the experimental group with mixed solution, data results such as following table.
Table 1:
Embodiment 2,
⑴, preparation high efficiency nitrification microbial inoculum:
The component of high efficiency nitrification microbial inoculum is:
Be 900ml with micrococcus sp A3, Corynebacterium A5 is 300ml, and Corynebacterium A11 is that 300ml is mixed and made into the high efficiency nitrification microbial inoculum, and high efficiency nitrification microbial inoculum pH is 6.7, and bacterial concentration is 10
8Individual/ml;
⑵, the efficient denitrification microbial inoculum of preparation: efficiently the component of denitrification microbial inoculum and compound proportion is:
Rhodopseudomonas A6 is 400ml; Brevibacterium sp N7 is 1400ml,, brevibacterium sp N3 is that 200ml is mixed and made into efficient denitrification microbial inoculum, and efficient denitrification microbial inoculum pH is 7.2, and bacterial concentration is 10
9Individual/ml;
⑶, to select useful volume be that anoxic pond and the useful volume of 50L is the Aerobic Pond of 50L.
Select and be COD:39mg/l, NH
3The sanitary wastewater 200Kg of-N water: 73mg/l.
With above-mentioned low COD high-ammonia-nitrogen sewage sanitary wastewater, be delivered to the anoxic pond that has added described efficient denitrification microbial inoculum, stopped 6 hours, the dosage first of efficient denitrification microbial inoculum is 4% of anoxic pond useful volume, be 2000ml, control pH6.0, control temperature: 30 ℃;
⑷, the sewage transport after hypoxia response are to the Aerobic Pond that has added described high efficiency nitrification microbial inoculum, stopped 2 hours, the dosage first of high efficiency nitrification microbial inoculum is 3% of Aerobic Pond useful volume, be 1500ml, control pH7.5, control temperature: 30 ℃, Aerobic Pond aeration rate and flooding quantity volume ratio are 22: 1;
⑸ the sewage backflow after, aerobic reaction, carries out denitrification and processes with the reaction of denitrification microbial inoculum to anoxic pond.
⑹, sewage enter Aerobic Pond again, carry out the nitrogen stripping, thereby realize the biological denitrificaion processing.
⑺ the continuous blow-down after the hollow fiber microfiltration membrane assembly of the sewage after, aerobic treatment.The hollow fiber microfiltration membrane assembly adopts curtain formula hollow fiber microfiltration membrane, and material is anti-pollution polyvinylidene fluoride, and membrane pore size is 0.1 μ m.
Aerobic Pond water outlet ammonia nitrogen concentration is monitored, do blank with the experimental group with mixed solution, data results such as following table.
Table 2:
Embodiment 3,
⑴, preparation high efficiency nitrification microbial inoculum:
The component of high efficiency nitrification microbial inoculum is:
Be 1000ml with micrococcus sp A3, Corynebacterium A5 is 750ml, and Corynebacterium A11 is that 750ml is mixed and made into the high efficiency nitrification microbial inoculum, and high efficiency nitrification microbial inoculum pH is 6.3, and bacterial concentration is 10
8Individual/ml;
⑵, the efficient denitrification microbial inoculum of preparation: efficiently the component of denitrification microbial inoculum and compound proportion is:
Rhodopseudomonas A6 is 1000ml; Brevibacterium sp N7 is 600ml,, brevibacterium sp N3 is that 400ml is mixed and made into efficient denitrification microbial inoculum, and efficient denitrification microbial inoculum pH scope is 7.0, and bacterial concentration is 10
9Individual/ml;
⑶, to select useful volume be that anoxic pond and the useful volume of 50L is the Aerobic Pond of 50L.
Select and be COD:87mg/l, NH
3The sanitary wastewater 200Kg of-N water: 168mg/l.
With above-mentioned low COD high-ammonia-nitrogen sewage sanitary wastewater, be delivered to the anoxic pond that has added described efficient denitrification microbial inoculum, stopped 8 hours, the dosage first of efficient denitrification microbial inoculum is 4% of anoxic pond useful volume, be 2000ml, control pH6.0, control temperature: 30 ℃;
⑷, the sewage transport after hypoxia response are to the Aerobic Pond that has added described high efficiency nitrification microbial inoculum, stopped 5 hours, the dosage first of high efficiency nitrification microbial inoculum is 5% of Aerobic Pond useful volume, be 2500ml, control pH7.5, control temperature: 30 ℃, Aerobic Pond aeration rate and flooding quantity volume ratio are 25: 1;
⑸ the sewage backflow after, aerobic reaction, carries out denitrification and processes with the reaction of denitrification microbial inoculum to anoxic pond.
⑹, sewage enter Aerobic Pond again, carry out the nitrogen stripping, thereby realize the biological denitrificaion processing.
⑺ the continuous blow-down after the hollow fiber microfiltration membrane assembly of the sewage after, aerobic treatment.The hollow fiber microfiltration membrane assembly adopts curtain formula hollow fiber microfiltration membrane, and material is anti-pollution polyvinylidene fluoride, and membrane pore size is 0.1 μ m.
Aerobic Pond water outlet ammonia nitrogen concentration is monitored, do blank with the experimental group with mixed solution, data results such as following table.
Table 3:
Claims (3)
1. one kind is utilized microorganism to carry out biological denitrification method to the low high ammonia nitrogen municipal effluent of COD, it is characterized in that: steps of the method are:
⑴, preparation high efficiency nitrification microbial inoculum: the component of high efficiency nitrification microbial inoculum and compound proportion be:
With micrococcus sp A3 1-6
Corynebacterium A5 1-4
Corynebacterium A11 2-5,
High efficiency nitrification microbial inoculum pH scope is at 4.0-8.0, and bacterial concentration is 10
6-10
9Individual/ml;
⑵, the efficient denitrification microbial inoculum of preparation: efficiently the component of denitrification microbial inoculum and compound proportion is:
Rhodopseudomonas A6 1-6
Brevibacterium sp N7 1-8
Brevibacterium sp N3 1-5,
Efficient denitrification microbial inoculum pH scope is at 4.0-8.0, and bacterial concentration is 10
6-10
9Individual/ml;
⑶, that efficient denitrification microbial inoculum is fed into anoxic pond is standby, and the dosage first of efficient denitrification microbial inoculum is the 0.5-20% of anoxic pond useful volume;
⑷, will hang down COD high-ammonia-nitrogen sewage sanitary wastewater and be delivered to anoxic pond, stop 6-24 hour, control pH5.0-9.0, control temperature: 20-40 ℃, carry out the acidication processing of sewage;
⑸, the sewage transport after hypoxia response stopped 2-24 hour to the Aerobic Pond that has added described high efficiency nitrification microbial inoculum,
The dosage first of high efficiency nitrification microbial inoculum is the 0.5-20% of Aerobic Pond useful volume, controls pH5.0-9.0, controls temperature: 20-40 ℃, and Aerobic Pond aeration rate and flooding quantity volume ratio are 15-25: 1, carry out nitrification;
⑹ the sewage backflow after, aerobic reaction, carries out denitrification and processes with the reaction of denitrification microbial inoculum to anoxic pond;
⑺, sewage enter Aerobic Pond again, carry out the nitrogen stripping, realize the biological denitrificaion processing;
⑻ the sewage continuous blow-down after the hollow fiber microfiltration membrane assembly after, secondary aerobic treatment.
2. profit according to claim 1 utilizes microorganism to carry out biological denitrification method to the low high ammonia nitrogen municipal effluent of COD, it is characterized in that: described hollow fiber microfiltration membrane assembly adopts curtain formula hollow fiber microfiltration membrane, material is anti-pollution polyvinylidene fluoride, and membrane pore size is 0.1-0.2 μ m.
3. according to claim 1ly utilize microorganism to carry out biological denitrification method to the low high ammonia nitrogen municipal effluent of COD, it is characterized in that: described low COD high-ammonia-nitrogen sewage sanitary wastewater is COD:1-100mg/l, NH
3The sanitary wastewater of-N water: 1-200mg/l.
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Cited By (5)
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| CN106115939A (en) * | 2016-07-28 | 2016-11-16 | 北京赛富威环境工程技术有限公司 | A kind of online bio-synergistic ammonia nitrogen removal method of sewage |
| CN108163997A (en) * | 2017-12-26 | 2018-06-15 | 纤化（上海）生物化工股份有限公司 | A kind of microorganism of dyeing and printing sewage denitrogenates method |
| CN108911132A (en) * | 2018-07-02 | 2018-11-30 | 南京万德斯环保科技股份有限公司 | Strengthen the method for biochemical reaction processing landfill leachate using microbial bacterial agent |
| CN109354189A (en) * | 2018-11-28 | 2019-02-19 | 广东莞绿环保工程有限公司 | A kind of microbial acclimation speed improving bio-refractory sewage and the biochemical processing method for reinforcing biochemical stablizing effect |
| CN112028243A (en) * | 2020-08-12 | 2020-12-04 | 深圳市广汇源环境水务有限公司 | Quick starting method of biological membrane system |
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| CN102443558A (en) * | 2011-12-21 | 2012-05-09 | 江苏江达生态科技有限公司 | Composite heterotrophic nitrifying bacterial agent and application of same in nitrogen removal treatment of waste water containing ammonia and nitrogen |
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| CN101962247A (en) * | 2010-08-18 | 2011-02-02 | 苏州英特工业水处理工程有限公司 | Composite biological denitrification treatment method for ammonia-nitrogen wastewater |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106115939A (en) * | 2016-07-28 | 2016-11-16 | 北京赛富威环境工程技术有限公司 | A kind of online bio-synergistic ammonia nitrogen removal method of sewage |
| CN108163997A (en) * | 2017-12-26 | 2018-06-15 | 纤化（上海）生物化工股份有限公司 | A kind of microorganism of dyeing and printing sewage denitrogenates method |
| CN108911132A (en) * | 2018-07-02 | 2018-11-30 | 南京万德斯环保科技股份有限公司 | Strengthen the method for biochemical reaction processing landfill leachate using microbial bacterial agent |
| CN109354189A (en) * | 2018-11-28 | 2019-02-19 | 广东莞绿环保工程有限公司 | A kind of microbial acclimation speed improving bio-refractory sewage and the biochemical processing method for reinforcing biochemical stablizing effect |
| CN112028243A (en) * | 2020-08-12 | 2020-12-04 | 深圳市广汇源环境水务有限公司 | Quick starting method of biological membrane system |
| CN112028243B (en) * | 2020-08-12 | 2023-02-03 | 深圳市广汇源环境水务有限公司 | Quick starting method of biological membrane system |
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Application publication date: 20130529 |