CN114835269B - Application of heterotrophic nitrification-aerobic denitrification composite microbial inoculant as bacterial and algal symbiotic aerobic granular sludge enhancer and method - Google Patents

Application of heterotrophic nitrification-aerobic denitrification composite microbial inoculant as bacterial and algal symbiotic aerobic granular sludge enhancer and method Download PDF

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CN114835269B
CN114835269B CN202210646042.7A CN202210646042A CN114835269B CN 114835269 B CN114835269 B CN 114835269B CN 202210646042 A CN202210646042 A CN 202210646042A CN 114835269 B CN114835269 B CN 114835269B
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aerobic
granular sludge
microbial inoculum
composite microbial
algae
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CN114835269A (en
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张千
秦树敏
龙昆
杨晨曦
赵婷婷
张楚
赵天涛
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Chongqing University of Technology
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    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/02Aerobic processes
    • C02F3/12Activated sludge processes
    • C02F3/1236Particular type of activated sludge installations
    • C02F3/1263Sequencing batch reactors [SBR]
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/32Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
    • C02F3/322Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
    • C02F3/325Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae as symbiotic combination of algae and bacteria
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00Controlling or monitoring parameters in water treatment
    • C02F2209/08Chemical Oxygen Demand [COD]; Biological Oxygen Demand [BOD]
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00Controlling or monitoring parameters in water treatment
    • C02F2209/14NH3-N
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00Controlling or monitoring parameters in water treatment
    • C02F2209/16Total nitrogen (tkN-N)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00Technologies for wastewater treatment
    • Y02W10/10Biological treatment of water, waste water, or sewage

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Water Supply & Treatment (AREA)
  • Hydrology & Water Resources (AREA)
  • Environmental & Geological Engineering (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Chemical & Material Sciences (AREA)
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  • Biotechnology (AREA)
  • Ecology (AREA)
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  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)

Abstract

The invention discloses application of a heterotrophic nitrification-aerobic denitrification composite microbial inoculum as a bacterial symbiotic aerobic granular sludge enhancer and a method thereof.

Description

Application of heterotrophic nitrification-aerobic denitrification composite microbial inoculant as bacterial and algal symbiotic aerobic granular sludge enhancer and method
Technical Field
The invention relates to the field of sewage treatment, in particular to application and a method of a heterotrophic nitrification-aerobic denitrification composite microbial inoculant serving as a bacterial and algal symbiotic aerobic granular sludge enhancer.
Background
The algae symbiotic aerobic granular sludge technology is a novel sewage treatment technology which appears in recent years, and has the advantages of small occupied area, high automation degree, low energy and material consumption in operation, capability of realizing carbon emission reduction and the like, so that the algae symbiotic aerobic granular sludge technology is widely paid attention to. However, this technique has the following problems in practical applications: (1) Sequencing Batch Reactor (SBR) based on the algae symbiotic aerobic granular sludge technology has serious algae loss in the running process, and needs to throw algae regularly to influence the stability of the system; (2) In the running process of a Sequencing Batch Reactor (SBR) based on the algae symbiotic aerobic granular sludge technology, the particle size of the aerobic granular sludge is continuously reduced along with the running time, even the particle size is broken, and the stability and the treatment effect of a system are influenced, which is one of key factors influencing the application of the aerobic granular sludge technology; (3) Sequencing Batch Reactor (SBR) based on the algae symbiotic aerobic granular sludge technology has poor impact load resistance, and particularly has poor high ammonia nitrogen resistance under the condition that the concentration of ammonia nitrogen in the inlet water suddenly increases, and the stability and the treatment effect of the system are affected; (4) When a Sequencing Batch Reactor (SBR) based on the algae symbiotic aerobic granular sludge technology is used for treating low-carbon source wastewater, the total nitrogen removal rate of the system is lower due to the invalid oxidation of part of carbon sources under the aerobic condition, and the system needs to be further improved.
Disclosure of Invention
In view of the above, one of the purposes of the present invention is to provide an application of a heterotrophic nitrification-aerobic denitrification composite microbial inoculum as a mycotic symbiotic aerobic granular sludge enhancer, which solves the problem of algae loss, the problem of aerobic granular sludge crushing, the problem of poor high ammonia nitrogen resistance of the system and the problem of low total nitrogen removal rate of the system under the low carbon condition by adding the heterotrophic nitrification-aerobic denitrification composite microbial inoculum into the mycotic symbiotic aerobic granular sludge system.
In order to achieve the above purpose, the present invention provides the following technical solutions:
the heterotrophic nitrification-aerobic denitrification composite microbial inoculum is formed by compounding copper bacteria (cupravidus sp) SWA1, alcaligenes faecalis (Alcaligenes faecalis), acinetobacter and pallidum (Ochroobacterium sp) TAC-2.
Preferably, the enhancer is used for reducing algae loss, reducing aerobic granular sludge fragmentation or/and improving the total nitrogen removal rate.
2. A method for treating sewage by using heterotrophic nitrification-aerobic denitrification composite microbial inoculum as a bacterial symbiotic aerobic granular sludge reinforcing agent comprises the steps of adding the heterotrophic nitrification-aerobic denitrification composite microbial inoculum into a bacterial symbiotic aerobic granular sludge system sequencing batch reactor for treating the sewage in a stirring aeration stage, wherein the heterotrophic nitrification-aerobic denitrification composite microbial inoculum is formed by compounding copper bacteria (cupriadus sp) SWA1, alcaligenes faecalis (Alcaligenes faecalis), acinetobacter and pallidum (Ochrobacter sp) TAC-2.
Preferably, the heterotrophic nitrification-aerobic denitrification composite microbial inoculum comprises copper bacteria (cupravudus sp) SWA1, alcaligenes faecalis (Alcaligenes faecalis), acinetobacter (Acinetobacter) and pallidum TAC-2 with a composite ratio of 10-20 percent: 5-20%: 10-30%: 20-50%.
Preferably, the operation condition of the sequencing batch reactor is that the operation period is 6 hours, the inflow is 4-8 minutes, the stirring aeration is 5.3-5.5 hours, the precipitation is 5-15 minutes, the discharge is 3-5 minutes, and the standby is 6-16 minutes.
Preferably, the adding volume of the composite microbial inoculum accounts for 20-40% of the volume of the treated water, and the density OD of the bacteria is the same as that of the treated water 600 1 to 1.2, and the adding times are 3 to 5 times.
Preferably, the wastewater is rural domestic wastewater, the C/N is less than 3, the ammonia nitrogen is 20-120mg/L, the COD is 60-350mg/L, and the total nitrogen is 30-150 mg/L.
Preferably, the bacterial and algal symbiotic aerobic granular sludge system contains aerobic granular sludge and algae, the concentration of the aerobic granular sludge is 2000-10000mg/L, the particle size range is 450-550 mu m, the algae is FACHB-9 protein chlorella pyrenoidosa, and the density of the chlorella is OD 680 And represents a range of 1.5 to 1.7.
Preferably, the mass ratio of the algae to the aerobic granular sludge is 1:6-1:4.
The invention has the beneficial effects that: the invention discloses application of heterotrophic nitrification-aerobic denitrification composite microbial inoculum as a bacteria-algae symbiotic aerobic granular sludge reinforcing agent, wherein the average removal rate of total nitrogen is improved from 55.6% to 88.7% by adding the composite microbial inoculum into sewage with the C/N ratio less than 3, the average removal rate is improved by 33.1%, and the effect is improved remarkably; the problem that the granular sludge is easy to break is solved, the average grain size of the granular sludge of a control group is reduced by 14.9% along with the running time, and the average grain size of the granular sludge of an experimental group is increased by 16.6% along with the running time; the algae loss problem can be remarkably improved, the control algae loss rate reaches 59.7% along with the running time, the experimental group algae loss rate is 24.3% along with the running time, and the loss rate is reduced by 35.4%; the tolerance of the control group to high ammonia nitrogen and the total nitrogen removal capacity can be remarkably improved, the total nitrogen removal of the control group is sharply reduced when the initial ammonia nitrogen concentration exceeds 200mg/L, the experimental group still keeps higher total nitrogen removal under the condition of high ammonia nitrogen, the average total nitrogen removal rate of the control group is 35.2% under the condition of 300mg/L of initial ammonia nitrogen, the average total nitrogen removal rate of the experimental group is 78.6%, and 43.4% is improved compared with the control group, so that the sewage treatment capacity can be improved by adding the microbial inoculum.
Drawings
In order to make the objects, technical solutions and advantageous effects of the present invention more clear, the present invention provides the following drawings for description:
FIG. 1 is a process flow diagram of a control group (the mycotic symbiotic aerobic granular sludge SBR) and an experimental group (the addition of the composite microbial inoculum).
FIG. 2 is a comparison of the removal effect of SBR reactors on contaminants before and after bio-enhancement of the composite microbial agents.
FIG. 3 is a comparison of the particle size of the granular sludge in the SBR reactor before and after bio-enhancement of the composite microbial agent with the change of the running time.
FIG. 4 is a comparison of algae concentration in SBR reactors before and after bio-enhancement of the composite microbial agent with time of operation.
FIG. 5 is a comparison of the removal effect of SBR reactors on contaminants at different initial ammonia nitrogen concentrations before and after bio-enhancement of the composite microbial agent.
Detailed Description
The present invention will be further described with reference to the accompanying drawings and specific examples, which are not intended to limit the invention, so that those skilled in the art may better understand the invention and practice it.
The composite bacterial agent TA-1 is formed by compounding copper bacteria (cupravudus sp) SWA1, alcaligenes faecalis (Alcaligenes faecalis), acinetobacter and pallidum (Ochroobacterium sp) TAC-2, wherein the compounding ratio is 10-20 percent: 5-20%: 10-30%: 20 to 50 percent, and the specific Chinese patent with the publication number of CN 109082387A.
Example 1
The treated wastewater is rural domestic sewage with C/N less than 3, ammonia nitrogen of 30-50mg/L and COD 100-200mg/L, total nitrogen 50-80mg/L; adding the algae symbiotic aerobic granular sludge system for treatment, wherein the operation period of the reactor is 6h (4 periods per day), and the method comprises the following steps: flowing into the reactor for 4min, reacting (stirring aeration) for 5.5h, precipitating for 5-15 min, discharging for 5min, and standing by; the concentration of aerobic granular sludge in the system is 2500mg/L, the particle size range is 470-480 mu m, the algae is FACHB-9 protein nuclear chlorella, and the density of the chlorella is OD 680 The range is 1.5, the composite bacterial agent TA-1 is added in the stirring aeration stage of each operation period in the operation process, the adding volume of the composite bacterial agent TA-1 is 25% of the volume of the treated water, and the density OD of bacteria is the same as that of the treated water 600 1, the number of times of bacterial feeding is 3, and the process flow is shown in figure 1.
In the embodiment, the algae is FACHB-9 protein chlorella pyrenoidosa, the initial inoculation algae and the aerobic granular sludge are inoculated according to the mass ratio of 1:5, and the density of the inoculated chlorella is OD 680 1.5, and after the reactor was stably operated, the treatment effect was detected.
The control group treatment experiment group is the same, except that the composite bacterial agent TA-1 is not added, and the treatment effect is shown in figures 2-5.
FIG. 2 is a comparison of the removal effect of SBR reactors on contaminants before and after bio-enhancement of the composite microbial agents. As shown in FIG. 2, the average removal rates of NH4+ -N, TN and TP of the control group are 96.3%, 55.6% and 72.03%, respectively, and the removal rates of NH4+ -N, TN and TP of the control group added with TA-1 bacteria can reach 98.8%, 88.7% and 80.4%, wherein the average removal rate of TN is improved by 33.1%, and the effect is improved remarkably.
FIG. 3 is a comparison of the particle size of the granular sludge in the SBR reactor before and after bio-enhancement of the composite microbial agent with the change of the running time. As can be seen from FIG. 3, the average particle size of the granular sludge in the AGS+algae system increases from 488 μm to 539 μm, the granular sludge starts to be broken up at 60h, the average particle size becomes 415 μm, the particle size of the granular sludge increases from 488 μm to 569 μm after the TA-1 bacteria is added into the system, and the granular sludge is stable. The average particle size of the granular sludge of the control group is reduced by 14.9% along with the running time, and the average particle size of the granular sludge of the experimental group is increased by 16.6% along with the running time.
FIG. 4 is a comparison of algae concentration in SBR reactors before and after bio-enhancement of the composite microbial agent with time of operation. As shown in FIG. 4, with the increase of the period, since the Chlorella pyrenoidosa had a small density and was difficult to settle, the concentration of algae began to be lost from the initial 0.72g/L to 0.29g/L after the addition of Chlorella pyrenoidosa to AGS. The loss rate of algae in the system after TA-1 bacteria is added is slowed down from the initial concentration of 0.75g/L to 0.56g/L, the loss rate of control algae in the system along with the running time reaches 59.7%, and the loss rate of experimental algae in the experimental group along with the running time is 24.3%, and the loss rate is reduced by 35.4%.
FIG. 5 is a comparison of the removal effect of SBR reactors on contaminants at different initial ammonia nitrogen concentrations before and after bio-enhancement of the composite microbial agent. As shown in FIG. 5, the total nitrogen removal rate of the control group is 35.2% under the initial ammonia nitrogen condition of 300mg/L, and the average total nitrogen removal rate of the control group is 78.6%, which is improved by 43.4% compared with the control group, so that the tolerance of the control group to high ammonia nitrogen and the total nitrogen removal capacity can be remarkably improved by adding the microbial inoculum.
OD for density of composite microbial inoculum TA-1 added in the invention 600 The volume ratio of the composite microbial inoculum to the treated water is 20-40%, and the addition frequency of the composite microbial inoculum is 3-5 times; aerobic granular sludge, the sludge concentration range of which in the SBR reactor is 2000-10000mg/L, and the particle size range of the granular sludge is 450-550 mu m; inoculating algae mud according to the mass ratio of 1: inoculating in the range of 6-1:4, and using OD for density of inoculated chlorella 680 It is indicated that the range is 1.5 to 1.7 (unit is 1). The invention can treat sewage with the concentration of C/N less than 3, the concentration of ammonia nitrogen ranging from 20mg/L to 120mg/L, the total nitrogen ranging from 30 mg/L to 150mg/L and the COD ranging from 60 mg/L to 350mg/L by the composite microbial inoculum TA-1.
The operation condition of the sequencing batch reactor is that the operation period is 6 hours, the flow is 4 to 8 minutes, the stirring aeration is 5.3 to 5.5 hours, the precipitation is 5 to 15 minutes, the discharge is 3 to 5 minutes, and the standby is 6 to 16 minutes; the adding volume of the composite microbial inoculum accounts for 20-40% of the volume of the treated water, and the density OD of the bacteria is equal to that of the treated water 600 1 to 1.2, and the adding times are 3 to 5 times; the wastewater is rural domestic wastewater, the C/N is less than 3, and the ammonia nitrogen is 20-120mg/LCOD is 60-350mg/L, total nitrogen is 30-150 mg/L; the algae symbiotic aerobic granular sludge system contains aerobic granular sludge and algae, the concentration of the aerobic granular sludge is 2000-10000mg/L, the particle size range is 450-550 mu m, the algae is FACHB-9 protein nucleus chlorella, and the density of the chlorella is OD 680 The range of 1.5 to 1.7 can achieve the object.
The above-described embodiments are merely preferred embodiments for fully explaining the present invention, and the scope of the present invention is not limited thereto. Equivalent substitutions and modifications will occur to those skilled in the art based on the present invention, and are intended to be within the scope of the present invention. The protection scope of the invention is subject to the claims.

Claims (5)

1. The application of the heterotrophic nitrification-aerobic denitrification composite microbial inoculant as a bacterial and algal symbiotic aerobic granular sludge enhancer is characterized in that: the heterotrophic nitrification-aerobic denitrification composite microbial inoculum is formed by compounding copper bacteria (cupriadus sp) SWA1, alcaligenes faecalis (Alcaligenes faecalis), acinetobacter (Acinetobacter) and pallidum (Ochrobacter sp) TAC-2; the composite microbial inoculum reduces algae loss, reduces aerobic granular sludge crushing and improves total nitrogen removal rate; the heterotrophic nitrification-aerobic denitrification composite microbial inoculum comprises copper bacteria (cupriadus sp) SWA1, alcaligenes faecalis (Alcaligenes faecalis), acinetobacter (Acinetobacter) and pallidum (Ochroobacterium sp) TAC-2 with a composite ratio of 10-20 percent: 5-20%: 10-30%: 20-50%; the algae symbiotic aerobic granular sludge system comprises aerobic granular sludge and algae, wherein the concentration of the aerobic granular sludge is 2000-10000mg/L, the particle size range is 450-550 mu m, the algae is FACHB-9 protein nucleus chlorella, and the density of the chlorella is OD 680 And represents a range of 1.5 to 1.7.
2. The method for treating sewage by using heterotrophic nitrification-aerobic denitrification composite microbial inoculum as a bacteria-algae symbiotic aerobic granular sludge enhancer is characterized by comprising the following steps of: in the stirring aeration stage, adding a heterotrophic nitrification-aerobic denitrification composite microbial inoculum into a bacterial and algal symbiotic aerobic granular sludge system sequencing batch reactor which is introduced into the wastewater treatment, wherein the heterotrophic nitrification-aerobic denitrification composite microbial inoculum is formed by compounding copper bacteria (cupriadus sp) SWA1, alcaligenes faecalis (Alcaligenes faecalis), acinetobacter and pallidum (Ochroobacterium sp) TAC-2; the heterotrophic nitrification-aerobic denitrification composite microbial inoculum comprises copper bacteria (cupriadus sp) SWA1, alcaligenes faecalis (Alcaligenes faecalis), acinetobacter (Acinetobacter) and pallidum (Ochroobacterium sp) TAC-2 with a composite ratio of 10-20 percent: 5-20%: 10-30%: 20-50%; the composite microbial inoculum reduces algae loss, reduces aerobic granular sludge crushing and improves total nitrogen removal rate; the mass ratio of the algae to the aerobic granular sludge is 1:6-1:4.
3. The method for treating sewage by using heterotrophic nitrification-aerobic denitrification complex microbial inoculum as a mycotic symbiotic aerobic granular sludge enhancer, which is characterized in that: the operation condition of the sequencing batch reactor is that the operation period is 6h, the flow is 4-8 min, the stirring aeration is 5.3-5.5h, the precipitation is 5-15 min, the discharge is 3-5min, and the standby is 6-16 min.
4. The method for treating sewage by using heterotrophic nitrification-aerobic denitrification complex microbial inoculum as a mycotic symbiotic aerobic granular sludge enhancer, which is characterized in that: the adding volume of the composite microbial inoculum accounts for 20-40% of the volume of the treated water, and the density OD of the bacteria is equal to that of the treated water 600 1 to 1.2, and the adding time is 3 to 5 times.
5. The method for treating sewage by using heterotrophic nitrification-aerobic denitrification complex microbial inoculum as a mycotic symbiotic aerobic granular sludge enhancer, which is characterized in that: the wastewater is rural domestic wastewater, the C/N is less than 3, the ammonia nitrogen is 20-120mg/L, the COD is 60-350mg/L, and the total nitrogen is 30-150 mg/L.
CN202210646042.7A 2022-06-09 2022-06-09 Application of heterotrophic nitrification-aerobic denitrification composite microbial inoculant as bacterial and algal symbiotic aerobic granular sludge enhancer and method Active CN114835269B (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104129845A (en) * 2013-07-15 2014-11-05 成都信息工程学院 Novel method for culturing aerobic denitrification granule sludge in continuous flow system
CN106045041A (en) * 2016-07-16 2016-10-26 北京工业大学 A<2>/O denitrifying phosphorus removal device and method for bi-granule sludge improvement
CN110697884A (en) * 2019-09-18 2020-01-17 山东大学 Method for culturing bacteria-algae symbiotic granular sludge at low temperature
CN111233166A (en) * 2020-01-19 2020-06-05 重庆理工大学 Method for biofilm formation of biofilm reactor by using microbial inoculum

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104129845A (en) * 2013-07-15 2014-11-05 成都信息工程学院 Novel method for culturing aerobic denitrification granule sludge in continuous flow system
CN106045041A (en) * 2016-07-16 2016-10-26 北京工业大学 A<2>/O denitrifying phosphorus removal device and method for bi-granule sludge improvement
CN110697884A (en) * 2019-09-18 2020-01-17 山东大学 Method for culturing bacteria-algae symbiotic granular sludge at low temperature
CN111233166A (en) * 2020-01-19 2020-06-05 重庆理工大学 Method for biofilm formation of biofilm reactor by using microbial inoculum

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