CN101830558B - Method for cultivating anaerobic ammonium oxidation granular sludge - Google Patents
Method for cultivating anaerobic ammonium oxidation granular sludge Download PDFInfo
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- CN101830558B CN101830558B CN2010101645212A CN201010164521A CN101830558B CN 101830558 B CN101830558 B CN 101830558B CN 2010101645212 A CN2010101645212 A CN 2010101645212A CN 201010164521 A CN201010164521 A CN 201010164521A CN 101830558 B CN101830558 B CN 101830558B
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- 239000010802 sludge Substances 0.000 title claims abstract description 56
- 238000000034 method Methods 0.000 title claims abstract description 22
- 230000003647 oxidation Effects 0.000 title claims abstract description 21
- 238000007254 oxidation reaction Methods 0.000 title claims abstract description 21
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 title claims abstract description 17
- 239000002351 wastewater Substances 0.000 claims abstract description 31
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims abstract description 15
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 claims abstract description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 4
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 4
- 239000001301 oxygen Substances 0.000 claims abstract description 4
- 239000008187 granular material Substances 0.000 claims description 20
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 claims description 16
- 239000002131 composite material Substances 0.000 claims description 9
- 230000001546 nitrifying effect Effects 0.000 claims description 7
- 229910052751 metal Inorganic materials 0.000 claims description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 4
- 229910052799 carbon Inorganic materials 0.000 claims description 4
- 239000002184 metal Substances 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 150000003016 phosphoric acids Chemical class 0.000 claims description 4
- 235000013619 trace mineral Nutrition 0.000 claims description 4
- 239000011573 trace mineral Substances 0.000 claims description 4
- 244000005700 microbiome Species 0.000 claims description 3
- 229910052796 boron Inorganic materials 0.000 claims description 2
- 229910052802 copper Inorganic materials 0.000 claims description 2
- 229910052742 iron Inorganic materials 0.000 claims description 2
- 229910052748 manganese Inorganic materials 0.000 claims description 2
- 229910052750 molybdenum Inorganic materials 0.000 claims description 2
- 229910052759 nickel Inorganic materials 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- 229910052725 zinc Inorganic materials 0.000 claims description 2
- 206010021143 Hypoxia Diseases 0.000 abstract description 2
- 230000000813 microbial effect Effects 0.000 abstract description 2
- 230000007954 hypoxia Effects 0.000 abstract 1
- 230000000696 methanogenic effect Effects 0.000 abstract 1
- 238000005086 pumping Methods 0.000 abstract 1
- 230000000087 stabilizing effect Effects 0.000 abstract 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 10
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- 229910021529 ammonia Inorganic materials 0.000 description 5
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 238000004065 wastewater treatment Methods 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 229910002651 NO3 Inorganic materials 0.000 description 3
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 3
- 229920002472 Starch Polymers 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000008107 starch Substances 0.000 description 3
- 235000019698 starch Nutrition 0.000 description 3
- 238000009280 upflow anaerobic sludge blanket technology Methods 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- IPQVRLSXWJPESU-UHFFFAOYSA-N [N].ON=O Chemical compound [N].ON=O IPQVRLSXWJPESU-UHFFFAOYSA-N 0.000 description 2
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 206010002660 Anoxia Diseases 0.000 description 1
- 241000976983 Anoxia Species 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 244000208060 Lawsonia inermis Species 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- 241001453382 Nitrosomonadales Species 0.000 description 1
- 230000007953 anoxia Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000002352 surface water Substances 0.000 description 1
Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
Landscapes
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention provides a method for cultivating anaerobic ammonium oxidation granular sludge. The method comprises the steps of: mixing and putting two or three kinds of anaerobic methanogenic granular sludge, nitrification aerobic granular sludge and hypoxia denitrification granular sludge into an upflow anaerobic reactor; pumping simulate waste water containing ammonia nitrogen and nitrite nitrogen to start; keeping the temperature of 25 to 35 DEG C in the reactor and the pH value of 7.5 to 8.0 without dissolved oxygen, and keeping hydraulic detention time of 8 to 24 hours; and stabilizing for a period of time to obtain the anaerobic ammonium oxidation granular sludge from the reactor. The invention has the advantages of having good hydraulic performance and microbial activity of the anaerobic ammonium oxidation granular sludge cultivated by the method, and greatly improving the operation effectiveness and the operation stability of the reactor.
Description
(1) technical field
The present invention relates to a kind of cultural method of anaerobic ammonium oxidation granular sludge, belong to the biological wastewater treatment technology field.
(2) background technology
According to China Environmental State Bulletin, China's ammonia nitrogen quantity discharged reached 149.8 ten thousand tons in 2005, increased by 12.6% than the last year.The whole nation surface water body ammonia nitrogen phenomenon ubiquity that exceeds standard, eutrophication is very serious, causes lake wawter bloom and bay red tide to take place frequently, and polluted by nitrogen control has become the current great environmental protection problem that needs to be resolved hurrily.
Under anoxia condition, utilize anaerobic ammonia oxidizing bacteria that nitrite and ammonia are changed into the process of nitrogen, be called Anammox.Anaerobic ammonia oxidation process is the new bio denitrification process of extremely people favor.This technology has been successfully applied to the processing of sewage work's digested sludge pressing filtering liquid, and its processing costs only is half of traditional biological denitrification process.
But because the Anammox bacteria growing is very slow, it it be unclear that in natural characteristic distributions, thereby ubiquity problems such as load is lower, clearance is on the low side, operational conditions requirement harshness in the anaerobic ammonia oxidation reactor of successful operation at present.Granule sludge is the particulate state microbial aggregate with good settling property that the bacterium self coagulation forms, and granule sludge is key and the prerequisite that realizes efficient operation in high efficiency anaerobic reactor such as UASB (upflow type anaerobic reactor), EGSB (expanded granular bed bioreactor) and IC (anaerobism inner circulation reactor) etc.
(3) summary of the invention
The objective of the invention is in order to solve the reactor volume load that causes owing to sludge concentration is low in the existing anaerobic ammonia oxidation reactor low low with clearance, and the single problems such as fluctuation of service that cause of flora structure, a kind of cultural method of anaerobic ammonium oxidation granular sludge is provided.
The technical solution used in the present invention is:
A kind of cultural method of anaerobic ammonium oxidation granular sludge, described method comprises:
(1) gets composite grain mud, pack into the be furnished with triphase separator upflow type anaerobic reactor of (effect of triphase separator is to prevent and treat a large amount of losses of kind of mud), described composite grain mud is the mixture that anaerobism is produced two or three in methane granule sludge, aerobic nitrifying granular activated sludge, the anoxic denitrification granule sludge; Described anaerobism is produced the active sludge of methane granule sludge from waste disposal plants such as Processing Paper Wastewater, starch wastewaters, described aerobic nitrifying granular activated sludge is from the active sludge of waste disposal plants such as Ammonia-Containing Wastewater Treatment, and described anoxic denitrification granule sludge contains the active sludge of waste disposal plants such as nitrate wastewater from processing;
(2) preparation contains ammonia nitrogen (promptly with NH
4 +The N that form exists) and nitrite nitrogen (promptly with NO
2 -The N that form exists) simulated wastewater starts reactor, and an amount of routine of interpolation is used to keep microorganism normal growth and metabolic inorganic carbon source, phosphoric acid salt, light metal element and trace element in simulated wastewater; Ammonia nitrogen concentration is 70～1000mg/L in the described simulated wastewater, and the ratio of ammonia nitrogen and nitrite nitrogen is 0.8～1.3: 1;
(3) described simulated wastewater is pumped into described upflow type anaerobic reactor, the simulated wastewater described composite grain mud of flowing through is flowed out from reactor top, the part effluent recycling, reactor waterpower upflow velocity is controlled to be 1～3m/h, has certain rate of expansion to guarantee the granule sludge bed;
(4) keeping reactor temperature is 25～35 ℃, pH7.5～8.0, and no dissolved oxygen, hydraulic detention time are 8～24 hours, and steady running obtained described anaerobic ammonium oxidation granular sludge after 140～180 days in reactor.
In anaerobic ammonia oxidation reactor, turn out anaerobic ammonium oxidation granular sludge, can improve the biomass in the reactor, improve treatment efficiency with greater activity.The present invention adopts multiple granule sludge as kind of a mud, has enriched the flora structure of the granule sludge of cultivating, and has guaranteed the stability of reactor operation.
Preferably, composite grain mud is the mixture that anaerobism is produced methane granule sludge, aerobic nitrifying granular activated sludge, anoxic denitrification granule sludge mass ratio 1: 0.1～2: 1～5 in the described step (1).
Preferably, composite grain mud is the mixture that anaerobism is produced methane granule sludge and aerobic nitrifying granular activated sludge or anoxic denitrification granule sludge mass ratio 1: 1～10 in the described step (1).
Inorganic carbon source is NaHCO in the described step (2)
3, KHCO
3, Na
2CO
3, K
2CO
3In one or more, addition is 0.05～0.15g/L (in C) simulated wastewater.
Phosphoric acid salt is K in the described step (2)
2HPO
4, KH
2PO
4, Na
2HPO
4, NaH
2PO
4In one or more, addition is 0.005～0.01g/L (in P) simulated wastewater.
The light metal element is Ca, K, Na and Mg in the described step (2), every kind of element addition 0.001～0.03mg/L (in element) simulated wastewater of doing for oneself.
Trace element is one or more among Fe, B, Mn, Cu, Co, Zn, Ni, Mo, the Se in the described step (2), every kind of element addition 0.002～1.8mg/L (in element) simulated wastewater of doing for oneself.
Anaerobic ammonium oxidation granular sludge of the present invention mainly can be applicable to contain the processing of ammonia nitrogen or nitrite nitrogen waste water.
Beneficial effect of the present invention is mainly reflected in: the anaerobic ammonium oxidation granular sludge of turning out according to the method for the invention has good hydraulic performance and microorganism active, and the operation usefulness and the operation stability of reactor all are greatly improved.
(4) embodiment
The present invention is described further below in conjunction with specific embodiment, but protection scope of the present invention is not limited in this:
Embodiment 1:
The mass ratio of aerobic nitrifying granular activated sludge in certain air lift type nitrator of anaerobism in certain IC reactor of Processing Paper Wastewater product methane granule sludge and Ammonia-Containing Wastewater Treatment (ammonia nitrogen concentration 700mg/L) by 1: 1 mixed.Then the composite grain sludge seeding in upflow type anaerobic reactor, the cumulative volume of this reactor is 6.0L, wherein the reaction zone volume is 3.6L, is furnished with triphase separator in the settling region, the sludge concentration of inoculation in the post-reactor is 23.2g SS/L.Ammonia nitrogen concentration is 200mg/L in the simulated wastewater, and ammonia nitrogen is 1 with the ratio of nitrous acid nitrogen, has also added KHCO in the above-mentioned in addition waste water
3(0.05g/L is in C), KH
2PO
4(0.006g/L, in P), micro-Fe 1.8mg/L, B 0.002mg/L, Mn 0.28mg/L, Cu 0.06mg/L, Co 0.10mg/L, Zn 0.10mg/L, Ni 0.05mg/L, Mo 0.10mg/L, Se 0.05mg/L, and light metal Elements C a, K, Na, each 0.01mg/L of Mg, do not contain organism in the simulated wastewater.The operational condition of reactor is: 30～35 ℃ of temperature, and pH 7.6～8.0, no dissolved oxygen, hydraulic detention time 8～24 hours, the waterpower upflow velocity is controlled at 1～3m/h.Steady running was observed henna anaerobic ammonium oxidation granular sludge in the post-reactor of the left and right sides in 180 days.The main particle size range of granule sludge is 0.3～1.4mm, and the average settlement speed of granule sludge is 69.6m/h; Anaerobic ammoxidation activity is about 0.5kgN/ (kg VSS.d).Continue operation 80 days, at volume total nitrogen load 10.5kg/ (m
3.d) under the condition, the clearance of ammonia nitrogen and nitrous acid nitrogen all can reach more than 95%, and this reactor serviceability is good, and can tolerate 2 times of substrate concns impacts and 3 times of flow attacks, has higher operation stability.
Embodiment 2:
Even the denitrification granular sludge that the anaerobism in certain UASB reactor of handling starch wastewater is produced in the up-flow reactor that methane granule sludge and processing contain nitrate wastewater (the concentration of nitrate nitrogen 280mg/L) by 1: 2 mixed.All the other operating process are identical with embodiment 1, and cultured continuously anaerobic ammonium oxidation granular sludge occurred in the post-reactor of the left and right sides in 150 days.
Embodiment 3:
Get aerobic nitrifying granular activated sludge in certain air lift type nitrator that anaerobism in certain UASB reactor of handling starch wastewater produces methane granule sludge, Ammonia-Containing Wastewater Treatment (ammonia nitrogen concentration 700mg/L) respectively, handle the denitrification granular sludge in the up-flow reactor that contains nitrate (the concentration of nitrate nitrogen 280mg/L) waste water, even by 1: 0.5: 2 mixed.All the other operating process are identical with embodiment 1, and cultured continuously anaerobic ammonium oxidation granular sludge occurred in the post-reactor of the left and right sides in 140 days.Continue operation 75 days, the reactor volume total nitrogen is removed speed can be up to 12.3kg/ (m
3.d).
Claims (5)
1. the cultural method of an anaerobic ammonium oxidation granular sludge, described method comprises:
(1) gets composite grain mud, pack into and be furnished with the upflow type anaerobic reactor of triphase separator, described composite grain mud is the mixture that anaerobism is produced methane granule sludge, aerobic nitrifying granular activated sludge, anoxic denitrification granule sludge mass ratio 1: 0.1～2: 1～5;
(2) preparation contains the simulated wastewater of ammonia nitrogen and nitrite nitrogen, and adds inorganic carbon source, phosphoric acid salt, light metal element and the trace element of keeping microorganism growth in right amount in simulated wastewater; Ammonia nitrogen concentration is 70～1000mg/L in the described simulated wastewater, and the ratio of ammonia nitrogen and nitrite nitrogen is 0.8～1.3: 1;
(3) described simulated wastewater is pumped into described upflow type anaerobic reactor, make simulated wastewater flow through described composite grain mud from the outflow of reactor top, reactor waterpower upflow velocity is controlled to be 1～3m/h;
(4) keeping reactor temperature is 25～35 ℃, pH7.5～8.0, and no dissolved oxygen, hydraulic detention time are 8～24 hours, and steady running obtained described anaerobic ammonium oxidation granular sludge after 140～180 days in reactor.
2. the method for claim 1 is characterized in that inorganic carbon source is NaHCO in the described step (2)
3, KHCO
3, Na
2CO
3, K
2CO
3In one or more, addition is counted 0.05～0.15g/L simulated wastewater with C.
3. the method for claim 1 is characterized in that phosphoric acid salt is K in the described step (2)
2HPO
4, KH
2PO
4, Na
2HPO
4, NaH
2PO
4In one or more, addition is counted 0.005～0.01g/L simulated wastewater with P.
4. the method for claim 1 is characterized in that the light metal element is Ca, K, Na and Mg in the described step (2), and every kind of element addition is in element quality 0.001～0.03mg/L simulated wastewater of doing for oneself.
5. the method for claim 1, it is characterized in that trace element in the described step (2) is among Fe, B, Mn, Cu, Co, Zn, Ni, Mo, the Se one or more, every kind of element addition is in element quality 0.002～1.8mg/L simulated wastewater of doing for oneself.
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|---|---|---|---|
| CN2010101645212A CN101830558B (en) | 2010-05-06 | 2010-05-06 | Method for cultivating anaerobic ammonium oxidation granular sludge |
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| CN101830558A CN101830558A (en) | 2010-09-15 |
| CN101830558B true CN101830558B (en) | 2011-12-21 |
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ID=42714801
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Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5791359B2 (en) * | 2011-05-02 | 2015-10-07 | 株式会社日立製作所 | Wastewater treatment method |
| CN102515348B (en) * | 2011-12-14 | 2013-08-07 | 杭州师范大学 | Operating method for high-efficient anaerobic ammonium oxidation reactor |
| CN103588293B (en) * | 2013-10-27 | 2015-05-27 | 沈阳建筑大学 | Control method of anaerobic ammoxidation reaction |
| CN104386813B (en) * | 2014-11-20 | 2016-01-13 | 沈阳建筑大学 | A kind of method of quick formation anaerobic ammonium oxidation granular sludge |
| CN104496017B (en) * | 2014-11-25 | 2016-04-06 | 山东省农业科学院农业资源与环境研究所 | A kind of method utilizing anaerobic filter to start Anammox fast |
| CN104843864A (en) * | 2015-06-02 | 2015-08-19 | 广西大学 | Method for improving stability of landfill leachate anaerobic granular sludge |
| CN105036321B (en) * | 2015-07-09 | 2017-05-03 | 杭州师范大学 | Method for culturing anammox sludge with high temperature resistance |
| CN105152327B (en) * | 2015-09-30 | 2017-11-24 | 杭州师范大学 | The cultural method of anaerobic ammonium oxidation granular sludge with hydroxyapatite kernel |
| CN105174448B (en) * | 2015-10-13 | 2017-05-31 | 沈阳建筑大学 | A kind of Anammox and denitrification coupling device and its control operation method |
| CN105923764A (en) * | 2016-06-27 | 2016-09-07 | 南京大学 | Method for quickly starting anaerobic ammonia oxidation technology by manganese dioxide |
| NL2021547B1 (en) * | 2018-09-03 | 2020-04-30 | Haskoningdhv Nederland Bv | Method of starting-up an anaerobic process |
| CN109748388A (en) * | 2019-03-11 | 2019-05-14 | 福建省环境科学研究院（福建省排污权储备和管理技术中心） | A method of attachment proliferous type inertia bio-carrier culture anaerobic ammonium oxidation granular sludge |
| CN110104768B (en) * | 2019-04-18 | 2021-12-21 | 杭州师范大学 | Culture method of nano-material-tolerant denitrifying sludge |
| CN111675438A (en) * | 2020-06-18 | 2020-09-18 | 黄河三角洲京博化工研究院有限公司 | Method for enriching anaerobic ammonium oxidation bacteria by utilizing tannery wastewater and activated sludge of biochemical system thereof |
| CN112047464B (en) * | 2020-08-04 | 2022-03-22 | 北京工业大学 | Culture method of tolerant low-dose fulvic acid anaerobic ammonium oxidation granular sludge |
| CN112093917B (en) * | 2020-09-29 | 2022-06-21 | 神美科技有限公司 | Rapid proliferation method of anaerobic ammonium oxidation bacteria |
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2010
- 2010-05-06 CN CN2010101645212A patent/CN101830558B/en not_active Expired - Fee Related
Non-Patent Citations (4)
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Effective date of registration: 20201124 Address after: No. 288, Changjiang West Road, hi tech Zone (former Hai'an town), Hai'an City, Nantong City, Jiangsu Province, 226600 Patentee after: Jiangsu Qingcai Intelligent Manufacturing Co.,Ltd. Address before: 226300 No. 266 Century Avenue, hi tech Zone, Jiangsu, Nantong Patentee before: NANTONG WOTE OPTOELECTRONICS TECHNOLOGY Co.,Ltd. Effective date of registration: 20201124 Address after: 226300 No. 266 Century Avenue, hi tech Zone, Jiangsu, Nantong Patentee after: NANTONG WOTE OPTOELECTRONICS TECHNOLOGY Co.,Ltd. Address before: Hangzhou City, Zhejiang province 310036 Xiasha Higher Education Park forest Street No. 16 Patentee before: Hangzhou Normal University |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20111221 |