CN109133366B - Culture method of anaerobic ammonium oxidation bacteria capable of tolerating nickel-containing high-ammonia-nitrogen salt-containing wastewater - Google Patents
Culture method of anaerobic ammonium oxidation bacteria capable of tolerating nickel-containing high-ammonia-nitrogen salt-containing wastewater Download PDFInfo
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- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 title claims abstract description 76
- 230000003647 oxidation Effects 0.000 title claims abstract description 37
- 238000007254 oxidation reaction Methods 0.000 title claims abstract description 37
- 239000002351 wastewater Substances 0.000 title claims abstract description 33
- 241000894006 Bacteria Species 0.000 title claims abstract description 31
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 title claims abstract description 30
- 229910052759 nickel Inorganic materials 0.000 title claims abstract description 21
- 238000012136 culture method Methods 0.000 title description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 59
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 34
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 29
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims abstract description 27
- 239000010802 sludge Substances 0.000 claims abstract description 26
- 229910017053 inorganic salt Inorganic materials 0.000 claims abstract description 25
- 238000000034 method Methods 0.000 claims abstract description 24
- 230000014759 maintenance of location Effects 0.000 claims abstract description 17
- 238000012258 culturing Methods 0.000 claims abstract description 11
- 239000011159 matrix material Substances 0.000 claims abstract description 9
- 150000003839 salts Chemical class 0.000 claims abstract description 8
- 238000011081 inoculation Methods 0.000 claims abstract description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical group [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 22
- 239000011573 trace mineral Substances 0.000 claims description 14
- 235000013619 trace mineral Nutrition 0.000 claims description 13
- 239000011780 sodium chloride Substances 0.000 claims description 12
- 239000000243 solution Substances 0.000 claims description 12
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 claims description 9
- 238000002360 preparation method Methods 0.000 claims description 9
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 8
- 229910021529 ammonia Inorganic materials 0.000 claims description 8
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 6
- 229910021586 Nickel(II) chloride Inorganic materials 0.000 claims description 6
- 239000007853 buffer solution Substances 0.000 claims description 6
- QMMRZOWCJAIUJA-UHFFFAOYSA-L nickel dichloride Chemical compound Cl[Ni]Cl QMMRZOWCJAIUJA-UHFFFAOYSA-L 0.000 claims description 6
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 4
- 239000001110 calcium chloride Substances 0.000 claims description 4
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 4
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 4
- 238000011084 recovery Methods 0.000 claims description 4
- 239000000758 substrate Substances 0.000 claims description 4
- 229910021580 Cobalt(II) chloride Inorganic materials 0.000 claims description 3
- 229910021380 Manganese Chloride Inorganic materials 0.000 claims description 3
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 claims description 3
- 229910018890 NaMoO4 Inorganic materials 0.000 claims description 3
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 3
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 3
- 239000011565 manganese chloride Substances 0.000 claims description 3
- 239000011736 potassium bicarbonate Substances 0.000 claims description 3
- 229910000028 potassium bicarbonate Inorganic materials 0.000 claims description 3
- TYJJADVDDVDEDZ-UHFFFAOYSA-M potassium hydrogencarbonate Chemical compound [K+].OC([O-])=O TYJJADVDDVDEDZ-UHFFFAOYSA-M 0.000 claims description 3
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 3
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 3
- 239000011686 zinc sulphate Substances 0.000 claims description 3
- 229910052927 chalcanthite Inorganic materials 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 239000002904 solvent Substances 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 12
- CVTZKFWZDBJAHE-UHFFFAOYSA-N [N].N Chemical compound [N].N CVTZKFWZDBJAHE-UHFFFAOYSA-N 0.000 abstract description 6
- 241001453382 Nitrosomonadales Species 0.000 abstract description 3
- 230000002411 adverse Effects 0.000 abstract description 2
- 244000005700 microbiome Species 0.000 description 6
- 229910001385 heavy metal Inorganic materials 0.000 description 4
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 3
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 description 3
- 229910052804 chromium Inorganic materials 0.000 description 3
- 239000011651 chromium Substances 0.000 description 3
- 230000002829 reductive effect Effects 0.000 description 3
- LPXPTNMVRIOKMN-UHFFFAOYSA-M sodium nitrite Chemical compound [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 238000011017 operating method Methods 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 231100000026 common toxicity Toxicity 0.000 description 1
- 229910000366 copper(II) sulfate Inorganic materials 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 230000002939 deleterious effect Effects 0.000 description 1
- 229910001873 dinitrogen Inorganic materials 0.000 description 1
- 150000003278 haem Chemical class 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 231100001252 long-term toxicity Toxicity 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 230000010254 physiological adaptation Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 238000011112 process operation Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- UIIMBOGNXHQVGW-UHFFFAOYSA-M sodium bicarbonate Substances [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000009280 upflow anaerobic sludge blanket technology Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/004—Apparatus and plants for the biological treatment of water, waste water or sewage comprising a selector reactor for promoting floc-forming or other bacteria
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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Abstract
The invention discloses a method for culturing anaerobic ammonium oxidation bacteria capable of tolerating nickel-containing high-ammonia nitrogen salt-containing wastewater, which comprises the following steps: inoculating anaerobic ammonium oxidation granular sludge serving as inoculation sludge into an anaerobic ammonium oxidation reactor; using simulated waste water containing Ni (II) and inorganic salt as inlet water and NH4 +-N and NO2 ‑N is water inlet matrix, the nitrogen volume load and the concentration of inorganic salt and Ni (II) in the reactor are controlled, and the culture is carried out by adopting a staged operation mode. The invention controls NH in the water inlet matrix4 +-N and NO2 ‑The method realizes the culture of anaerobic ammonia oxidizing bacteria resistant to nickel-containing high ammonia nitrogen salt-containing wastewater by N concentration, nitrogen volume load, Ni (II) concentration, inorganic salt concentration and hydraulic retention time in the reactor, provides a sludge acclimation method resistant to Ni (II) and inorganic salt, and improves the resistance of the reactor to adverse effects of the nickel-containing salt-containing wastewater.
Description
Technical Field
The invention relates to the technical field of cultivation of anammox bacteria, in particular to a cultivation method of anammox bacteria capable of tolerating nickel-containing high ammonia nitrogen salt-containing wastewater.
Background
Although trace amounts of heavy metals are ubiquitous in the organism and are often essential components of biological enzymes, excess amounts of heavy metals have inhibitory or even deleterious effects on the activity and growth of microorganisms. Anaerobic ammonia oxidation is used as a novel biological denitrification process, wherein anaerobic ammonia oxidizing bacteria serving as main missions are no exception, and the influence of heavy metals in wastewater can cause the quality of effluent water of the process to be rapidly deteriorated, the denitrification efficiency to be reduced, even thalli to be disintegrated and dead, and the process operation to be failed.
The industrial wastewater and municipal sludge often contain heavy metal Ni (II), and the nickel has toxic action after being excessive except for the trace elements for living for wastewater microorganisms; can block the metabolic processes of enzyme metabolism of anaerobic ammonium oxidation bacteria and the like, and obviously restrict the secretion of extracellular polymers of microorganisms.
At present, studies on the culture of tolerant anammox bacteria have been reported, for example:
(1) the invention patent application with the application publication number of CN101205526A discloses a method for quickly culturing anaerobic ammonia oxidizing bacteria by an upflow anaerobic sludge bed reactor, which comprises the steps of hanging a membrane in a UASB reactor and reserving enriched microorganisms; selecting activated sludge in the leachate SBR process for treating the refuse landfill as inoculated sludge, wherein the inoculated sludge accounts for 45-65% of the effective volume of the reactor; controlling the temperature of the reaction area to be kept at 32 +/-1 ℃ by adopting constant-temperature circulating water bath; adopting artificially synthesized wastewater, wherein the components of the wastewater comprise NH4Cl、NaNO2、MgSO4、KH2PO4、CaCl2、NaHCO3Trace elements I and II, NH in water4 +-N and NO2 -The mass ratio of-N is controlled to be 1: 1.0-1: 1.6; adjusting the pH value of the inlet water to 7.4-7.8.
(2) The invention patent application with application publication number CN105753150A discloses a culture method of chromium-resistant anaerobic ammonium oxidation granular sludge, which adopts an upflow anaerobic sludge bed reactor, takes anaerobic ammonium oxidation granular sludge as an inoculation source, takes simulated wastewater containing ammonia nitrogen and nitrite nitrogen as inlet water, cultures the anaerobic sludge bed reactor in a dark place at the temperature of 35 +/-1 ℃, the pH of the inlet water of 8.10 +/-0.14 and the hydraulic retention time of 1.0 to 1.5h until the denitrification efficiency of the reactor is stabilized to be more than 80 percent, and then K is added into the inlet water of the reactor2CrO4And performing chromium-resistant culture to finish the culture of the chromium-resistant anaerobic ammonia oxidation granular sludge.
However, no report is found on the culture method of the anaerobic ammonium oxidation bacteria which can tolerate the nickel-containing high ammonia nitrogen salt-containing wastewater.
Disclosure of Invention
The invention provides a culture method of anammox bacteria capable of tolerating nickel-containing high ammonia nitrogen salt-containing wastewater, and the anammox bacteria obtained by the method has strong tolerance capability to the nickel-containing high ammonia nitrogen salt-containing wastewater.
The specific technical scheme is as follows:
a method for culturing anaerobic ammonium oxidation bacteria capable of tolerating nickel-containing high-ammonia nitrogen salt-containing wastewater comprises the following steps:
(1) inoculating anaerobic ammonium oxidation granular sludge serving as inoculation sludge into an anaerobic ammonium oxidation reactor;
(2) using simulated waste water containing Ni (II) and inorganic salt as inlet water and NH4 +-N and NO2 -N is a water inlet substrate, the volume load of nitrogen and the concentrations of inorganic salt and Ni (II) in the reactor are controlled, and anaerobic ammonium oxidation bacteria are cultured in a staged operation mode, and the method comprises the following steps:
(a) the first stage is as follows: retention of NH in water-entering matrix4 +The concentration of-N is always 70-280 mg L-1，NO2 -The concentration of-N is always 70-280 mg L-1And NH4 +-N and NO2 --the ratio of the molar masses of N is 1: 1; to maintain NH in the effluent4 +-N and NO2 -The content of-N is 10mg L-1The following aims are to control the initial nitrogen volume load and the hydraulic retention time and adjust the concentration of Ni (II) to be 0.1-0.3 mg L-1Until the reactor runs stably;
(b) and a second stage: keeping the concentration of Ni (II) at 0.1-0.3 mg L-1Adding 2-3 g/L inorganic salt, and maintaining NH in the effluent after the reactor operates stably4 +-N and NO2 -The content of-N is 10mg L-1The following aims are to control the nitrogen volume load, prolong the hydraulic retention time and continuously increase the concentration of inorganic salt to 18-20 mg L-1；
(c) And a third stage: second stage to NH4 +-N and NO2 --N concentrations higher than 200mg L-1Then, entering the third stage, keeping the concentration of Ni (II) at 0.1-0.3 mg L-1Reducing the concentration of inorganic salts to 0mg L-1Allowing the reactor to enter a recovery period; adjusting NH in the influent substrate during recovery4 +-N and NO2 -The concentrations of-N are all 70mg L-1Gradually increased to 280mg L-1Controlling the volume load of nitrogen and the retention time of water power to maintain NH in the effluent4 +-N and NO2 -The content of-N is 10mg L-1The reactor was operated until the reactor was stable.
Anammox is catalyzed by a unique class of obligate anaerobic microorganisms called "anammox" which are members of the phylum bacteraria, in the process of oxidizing ammonia to nitrogen gas with nitrite as an electron acceptor under anaerobic conditions.
In the operation process of the anaerobic ammonia oxidation system, Ni (II) mainly has the effects of stimulating microorganisms to generate a large amount of heme, increasing biosorption and reducing toxicity so as to resist the penetration and long-term toxicity of Ni (II) accumulated in later operation to cells and ensure that the cells maintain certain activity; meanwhile, the physiological adaptation of the anammox bacteria to salinity may also be flora evolution, namely, the halotolerant bacteria gradually become dominant bacteria, and then the control of nitrogen volume load at different stages is matched, so that the anammox sludge is adapted to treat the wastewater containing Ni (II) salt and high ammonia nitrogen, and the high operation stability of the reactor is maintained.
Since anammox bacteria are very sensitive to Ni (II) and salts, the feed water contains 0.2mg L-1Ni (II) and 2.5mg L-1The salt can already make the anaerobic ammonium oxidation bacteria in the reactor have inhibition effect.
Preferably, in the step (1), the initial concentration of the anaerobic ammonia oxidation granular sludge is 12-30 g L-1(ii) a In the step (2), the pH value of the inlet water is 7.5-8.3.
Preferably, the Ni (II) is added in NiCl form2·6H2O, NiS or NiO; more preferably, Ni (II) is added in the form of NiCl2·6H2O。
Preferably, the inorganic salt is sodium chloride.
Preferably, in step (a), the nitrogen volume loading is 6.5 to 7.5kg Nm-3d-1The hydraulic retention time is 1-2 h.
Preferably, in step (b), the nitrogen volume loading is 6.5 to 7.5kg Nm-3d-1The hydraulic retention time is 7-9 h; after the concentration of the inorganic salt is adjusted each time, the operation period of the reactor is 14-25 days.
In step (c), the nitrogen volume loading1.0 to 2.0kg Nm-3d-1The hydraulic retention time is 7-9 h; each time NH is adjusted4 +-N and NO2 -And after the concentration of N is below zero, the operation period of the reactor is 14-25 days.
The method can effectively realize NH in water4 +-N and NO2 -The content of-N is 10mg L-1The following objects are achieved.
Preferably, an inorganic salt buffer solution is further added into the inlet water; the final concentration composition of each component of the inorganic salt buffer solution is as follows: KH (Perkin Elmer)2PO4 8～10mg L-1，CaCl2·2H2O 5～6mg L-1，MgSO4·2H2O 290～310mg L-1，KHCO3 1240～1260mg L-1The solvent is water.
Preferably, 1.00-1.25 ml L is further added into the inlet water-1The trace element I is prepared into solution and 1.00-1.25 ml of L-1Preparing a solution of the trace element II;
the trace element I preparation solution comprises the following components: EDTA 5.0-6.0 gL-1，FeSO4 9.1～9.2g L-1；
The microelement II preparation solution comprises the following components: EDTA 15.0-16.0 gL-1，ZnSO4·7H2O 0.40～0.45gL-1，CoCl2·6H2O 0.20～0.25gL-1，MnCl2·4H2O 0.95～1.00gL-1，CuSO4·5H2O 0.20～0.25gL-1，NaMoO4·2H2O 0.20～0.25gL-1，NiCl2·6H2O 0.20～0.25gL-1，H3BO4 0.010～0.015gL-1。
Compared with the prior art, the invention has the following beneficial effects:
the invention controls NH in the water inlet matrix4 +-N and NO2 -The N concentration and the nitrogen volume load, Ni (II) concentration, inorganic salt concentration and hydraulic retention time in the reactor achieve tolerance to high nickel contentThe culture of anaerobic ammonium oxidation bacteria of ammonia nitrogen salt-containing wastewater provides a sludge domestication method for tolerating Ni (II) and inorganic salt and a reactor performance improvement strategy for treating the Ni (II) and inorganic salt-containing wastewater, and improves the capability of a reactor for resisting the adverse influence of the nickel-containing salt-containing wastewater.
Drawings
FIG. 1 shows the Specific Anammox Activity (SAA) at different run times in example 1.
FIG. 2 shows the concentration of Tri-N in the inlet and outlet water at different operation times in example 1.
FIG. 3 shows the total nitrogen removal rate NRE (%) and the total nitrogen removal load NRR (kg Nm) at different operation times in example 1-3d-1) The variation of (2).
FIG. 4 shows the pH change and the change of the stoichiometric ratio of inlet and outlet water at different running times in example 1.
Detailed Description
The present invention will be further described with reference to the following specific examples, which are only illustrative of the present invention, but the scope of the present invention is not limited thereto.
Example 1
A method for culturing anaerobic ammonium oxidation bacteria capable of tolerating nickel-containing high-ammonia nitrogen salt-containing wastewater comprises the following specific steps:
the average nitrogen removal rate is 6.5-7.5 kg N m-3d-1900mL of the granular sludge in a certain high-efficiency anaerobic ammonia oxidation reactor was inoculated into an upflow anaerobic sludge blanket reactor having an effective volume of 1.5L. The initial sludge concentration of the reactor is 12.45gL-1The pH value of inlet water is 8.14 +/-0.14, the reactor is placed in a constant temperature chamber with the temperature of 35 +/-1 ℃ and anaerobic and light-proof for stable operation for 14 days, and the volume load of nitrogen is stable at 6.70 +/-0.15 kg N m-3d-1About, the sludge activity is 587.12 + -17.9 mg N g-1VSS d-1。
Using simulated waste water as water inlet matrix, adding inorganic salt buffer solution and trace elements for maintaining microbial growth, controlling nitrogen volume load in reactor and adding Ni (II) (adding NiCl in this example)2·6H2O) is run in stages.
Wherein (NH)4)2SO4With NaNO2The addition was carried out in the amount required in each stage in a 1:1 ratio of the amounts of the substances. The final concentration of each component of the inorganic salt buffer solution in the inlet water is as follows: KH (Perkin Elmer)2PO4 10mg L-1，CaCl2·2H2O 5.6mg L-1，MgSO4·2H2O 300mg L-1，KHCO3 1250mg L-1。
The feed water is added with a trace element I preparation solution 1.25mlL-1And trace element II 1.25mlL-1The trace element I preparation solution and the trace element II preparation solution comprise the following specific components:
preparation of trace element I (gL)-1):EDTA 5.00，FeSO4 9.14。
Microelement II preparation solution (gL)-1):EDTA 15.0，ZnSO4·7H2O 0.430，CoCl2·6H2O 0.240，MnCl2·4H2O 0.990，CuSO4 5H2O 0.250，NaMoO4·2H2O 0.220，NiCl2·6H2O 0.210，H3BO40.014。
The operating method and ni (ii) and NaCl concentrations were set as follows:
(1) the first stage is as follows: test feed water base concentration of NH4 +-N 280mg L-1，NO2 --N 280mg L-1，NH4 +-N and NO2 -A nitrogen volume loading of 6.7kg Nm with a 1:1 ratio of-N-3d-1Reactor initiation NiCl2·6H2The adding concentration of O is set to be 0.2mg L-1The operation is continued for 14 days, the nitrogen removal load in the reactor is only reduced to 99.55 percent, and the activity of the anaerobic ammonium oxidation bacteria in the reactor is from 587.12 +/-17.9 mg N g-1VSS d-1Increase to 698.46 + -10.93 mg N g-1VSS d-1The increase is 120.06%, which is indicated to be 0.2mg L-1The addition of Ni (II) is helpful for improving the activity of anaerobic ammonia oxidation.
(2) And a second stage: the adding concentration of Ni (II) is set to be 0.2mg L-1Adding 2.5g L without changing-1NaCl, nitrogen removal load slightly decreased to 6.67. + -. 0.32kg N m-3d-1It is shown that the anammox bacteria in the reactor have been slightly inhibited, compared with the conventional single anaerobic ammonium oxidation bacteria 2.5g L-1NaCl or Ni (II) with concentration of 0.2mg L added independently-1Both the promotion and inhibition of the flora; after 14 days of operation, the HRT is increased from 1.8h to 8h, and the NaCl concentration is continuously increased to 5g L-1At the moment, the reactor runs stably, and the effluent matrix content is less than 10mg L-1The nitrogen removal load was 1.54. + -. 0.08kg Nm-3d-1However, the flora activity decreased to 19.36%.
(3) And a third stage: continuously maintaining the Ni (II) concentration at 0.2mg L-1The NaCl concentration is increased to 10g L without changing-1，15g L-1The operation performance is stable after the operation is carried out for two weeks, and the nitrogen removal load is about 1.58kg Nm-3d-1While the flora activity is reduced to 6.84 percent, the effluent nitrite nitrogen content is maintained at 10mg L in the period-1The following.
(4) A fourth stage: the concentration of Ni (II) is still maintained at 0.2mg L-1The NaCl concentration is increased to 20g L without changing-1The nitrogen removal load was 0.91. + -. 0.34kg Nm-3d-1In the operation process, the performance of the reactor is rapidly deteriorated, and water NH is discharged4 +-N and NO2 -N continuous climb, 16 days of operation, NH4 +-N and NO2 --N concentrations higher than 200mg L-1。
(5) The fifth stage: after 16 days of operation in the fourth stage, the NaCl concentration was brought to 0g L-1The matrix concentration was maintained at 280mg L-1The operation was continued for 6 days, and the performance of the reactor was not improved yet.
(6) The sixth stage: by NH4 +-N and NO2 --N at 70, 140, 210, 280mg L-1The nitrite nitrogen content of the effluent is maintained at 10mg L after each concentration operation for 14-25 days-1Then, the concentration of anammox was changed to 280mg L-1，Outlet water NH4 +-N and NO2 --N was maintained at 10mg L-1The following stable operation.
Illustrates that according to the operating method, at a Ni (II) concentration of 0.2mg L-1When the NaCl concentration is not changed, the NaCl concentration is increased to be less than 20g L by the staged addition-1(e.g., 15g L)-1) The anaerobic ammonium oxidation bacteria in the reactor have stable treatment performance on the wastewater containing Ni (II) salt and are 0.2mg L-1And 20g L-1The reactor is destabilized by the common toxicity of NaCl, but the toxicity is reversible to the anaerobic ammonium oxidation granular sludge, and the activity can be gradually recovered by gradually increasing the concentration of the matrix (such as 70, 140, 210 and 280).
Claims (7)
1. A method for culturing anaerobic ammonium oxidation bacteria capable of tolerating nickel-containing high-ammonia nitrogen salt-containing wastewater is characterized by comprising the following steps:
(1) inoculating anaerobic ammonium oxidation granular sludge serving as inoculation sludge into an anaerobic ammonium oxidation reactor; the initial concentration of the anaerobic ammonia oxidation granular sludge is 12-30 g L-1；
(2) Simulated wastewater containing Ni (II) and inorganic salt is used as inlet water, the pH of the inlet water is 7.5-8.3, and NH is used4 +-N and NO2 -N is a water inlet substrate, the volume load of nitrogen and the concentrations of inorganic salt and Ni (II) in the reactor are controlled, and anaerobic ammonium oxidation bacteria are cultured in a staged operation mode, and the method comprises the following steps:
(a) the first stage is as follows: retention of NH in water-entering matrix4 +The concentration of-N is always 70-280 mg L-1，NO2 -The concentration of-N is always 70-280 mg L-1And NH4 +-N and NO2 --the ratio of the molar masses of N is 1: 1; to maintain NH in the effluent4 +-N and NO2 -The content of-N is 10mg L-1The following aims are to control the initial nitrogen volume load and the hydraulic retention time and adjust the concentration of Ni (II) to be 0.1-0.3 mg L-1Until the reactor runs stably; the nitrogen volume load is 6.5-7.5 kg Nm-3d-1The hydraulic retention time is 1-2h；
(b) And a second stage: keeping the concentration of Ni (II) at 0.1-0.3 mg L-1Adding 2-3 g/L inorganic salt, and maintaining NH in the effluent after the reactor operates stably4 +-N and NO2 -The content of-N is 10mg L-1The following aims are to control the nitrogen volume load, prolong the hydraulic retention time and continuously increase the concentration of inorganic salt to 18-20 mg L-1(ii) a The nitrogen volume load is 6.5-7.5 kg Nm-3d-1The hydraulic retention time is 7-9 h;
(c) and a third stage: second stage to NH4 +-N and NO2 --N concentrations higher than 200mg L-1Then, entering the third stage, keeping the concentration of Ni (II) at 0.1-0.3 mg L-1Reducing the concentration of inorganic salts to 0mg L-1Allowing the reactor to enter a recovery period; adjusting NH in the influent substrate during recovery4 +-N and NO2 -The concentrations of-N are all 70mg L-1Gradually increased to 280mg L-1Controlling the volume load of nitrogen and the retention time of water power to maintain NH in the effluent4 +-N and NO2 -The content of-N is 10mg L-1Then, the reactor runs stably; the nitrogen volume load is 1.0-2.0 kg Nm-3d-1The hydraulic retention time is 7-9 h.
2. The method for culturing the anaerobic ammonium oxidation bacteria capable of resisting the nickel-containing high-ammonia-nitrogen salt-containing wastewater as claimed in claim 1, wherein the Ni (II) is added in the form of NiCl2·6H2O, NiS or NiO.
3. The method for culturing the anaerobic ammonium oxidation bacteria capable of resisting the nickel-containing high-ammonia-nitrogen salt-containing wastewater as claimed in claim 1, wherein the inorganic salt is sodium chloride.
4. The method for culturing the anaerobic ammonium oxidation bacteria capable of resisting the nickel-containing high-ammonia-nitrogen salt-containing wastewater as claimed in claim 1, wherein in the step (b), the operation period of the reactor is 14-25 days after the concentration of the inorganic salt is adjusted each time.
5. The method for culturing the anaerobic ammonium oxidation bacteria capable of resisting the nickel-containing high-ammonia-nitrogen salt-containing wastewater as claimed in claim 1, wherein in the step (c), NH is adjusted each time4 +-N and NO2 -And after the concentration of N is below zero, the operation period of the reactor is 14-25 days.
6. The method for culturing the anaerobic ammonium oxidation bacteria capable of resisting the nickel-containing high-ammonia-nitrogen salt-containing wastewater as claimed in claim 1, wherein an inorganic salt buffer solution is further added into the influent water; the final concentration composition of each component of the inorganic salt buffer solution is as follows: KH (Perkin Elmer)2PO4 8～10mg L-1，CaCl2·2H2O 5～6mg L-1，MgSO4·2H2O 290～310mg L-1，KHCO3 1240～1260mg L-1The solvent is water.
7. The method for culturing the anaerobic ammonium oxidation bacteria capable of resisting the nickel-containing high-ammonia-nitrogen salt-containing wastewater as claimed in claim 1, wherein 1.00-1.25 ml L of the wastewater is added into the influent water-1The trace element I is prepared into solution and 1.00-1.25 ml of L-1Preparing a solution of the trace element II;
the trace element I preparation solution comprises the following components: EDTA 5.0-6.0 gL-1，FeSO4 9.1～9.2gL-1；
The microelement II preparation solution comprises the following components: EDTA 15.0-16.0 gL-1，ZnSO4·7H2O 0.40～0.45gL-1，CoCl2·6H2O 0.20～0.25gL-1，MnCl2·4H2O 0.95～1.00gL-1，CuSO4·5H2O 0.20～0.25gL-1，NaMoO4·2H2O 0.20～0.25gL-1，NiCl2·6H2O 0.20～0.25gL-1，H3BO4 0.010～0.015gL-1。
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102849857A (en) * | 2012-04-10 | 2013-01-02 | 南京大学 | Method for treating high-salinity high-concentration ammonia-nitrogen wastewater |
| CN103979678A (en) * | 2014-03-14 | 2014-08-13 | 杭州师范大学 | Operation method of anaerobic ammonia oxidation reactor for processing high-salt wastewater |
| CN105036324A (en) * | 2015-08-07 | 2015-11-11 | 杭州师范大学 | Running method for improving copper-containing wastewater denitrification processing performance of anaerobic ammonia oxidation reactor |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102849857A (en) * | 2012-04-10 | 2013-01-02 | 南京大学 | Method for treating high-salinity high-concentration ammonia-nitrogen wastewater |
| CN103979678A (en) * | 2014-03-14 | 2014-08-13 | 杭州师范大学 | Operation method of anaerobic ammonia oxidation reactor for processing high-salt wastewater |
| CN105036324A (en) * | 2015-08-07 | 2015-11-11 | 杭州师范大学 | Running method for improving copper-containing wastewater denitrification processing performance of anaerobic ammonia oxidation reactor |
Non-Patent Citations (1)
| Title |
|---|
| The properties of anaerobic ammonium oxidation (anammox) granules:Roles of ambient temperature, salinity and calcium concentration;Bao-Shan Xing等;《Separation and Purification Technology》;20150501;第147卷;第312页 * |
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