CN106635923B - Preparation method of high-density salt-tolerant denitrifying bacteria agent suitable for wastewater treatment - Google Patents

Preparation method of high-density salt-tolerant denitrifying bacteria agent suitable for wastewater treatment Download PDF

Info

Publication number
CN106635923B
CN106635923B CN201710051575.XA CN201710051575A CN106635923B CN 106635923 B CN106635923 B CN 106635923B CN 201710051575 A CN201710051575 A CN 201710051575A CN 106635923 B CN106635923 B CN 106635923B
Authority
CN
China
Prior art keywords
salt
denitrifying bacteria
wastewater
preparation
fillers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201710051575.XA
Other languages
Chinese (zh)
Other versions
CN106635923A (en
Inventor
张彬彬
王开春
郭涛
刘德敏
何一群
崔庆兰
杨志林
田凤蓉
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bluestar Lehigh Engineering Institute
Original Assignee
Bluestar Lehigh Engineering Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bluestar Lehigh Engineering Institute filed Critical Bluestar Lehigh Engineering Institute
Priority to CN201710051575.XA priority Critical patent/CN106635923B/en
Publication of CN106635923A publication Critical patent/CN106635923A/en
Application granted granted Critical
Publication of CN106635923B publication Critical patent/CN106635923B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Hydrology & Water Resources (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention relates to a preparation method of a high-density salt-tolerant denitrifying bacteria agent suitable for wastewater treatment. The microbial inoculum is resistant to high osmotic pressure and high salinity environment, the denitrification effect of the microbial inoculum is not influenced by 1-10% of salt concentration, nitrate nitrogen and nitrite nitrogen in salt-containing wastewater can be effectively removed, synchronous nitrification and denitrification of the high-salt nitrogen-containing wastewater are realized, and the problem that total nitrogen is difficult to reach the standard under the high-salt condition can be solved. The high-density salt-tolerant denitrifying bacteria agent prepared by the invention can be used for biological treatment of high-salt ammonia nitrogen-containing wastewater such as food processing, biological brewing, pesticides, leather and the like.

Description

Preparation method of high-density salt-tolerant denitrifying bacteria agent suitable for wastewater treatment
Technical Field
The invention belongs to the technical field of environmental protection microorganisms and sewage treatment, and particularly relates to a preparation method of a high-density salt-resistant denitrifying bacteria agent suitable for wastewater treatment.
Background
The high-salinity wastewater generally refers to wastewater with the total salt content of at least 1 percent by mass, seawater substitution and other industrial practical processes, the salt content sometimes reaches as high as 15-30 percent, the output path and the yield of the high-salinity wastewater are continuously increased at present, the water quantity of the wastewater is increased year by year at the annual increase rate of 3 percent, and if the high-salinity wastewater cannot reach the discharge standard, the high-salinity wastewater pollutes the earth surface, soil, coastal areas, river mouths and the like, so that various environmental problems and ecological problems such as eutrophication are caused. In the research on the biological denitrification performance of high-salinity wastewater, most of the research is directed at ammonia nitrogen removal, but the research on the removal of total nitrogen is less, ammonia nitrogen often reaches the standard in many sewage treatment plants and practical engineering applications, nitrate and nitrite cannot be effectively removed due to various reasons such as low activity of denitrifying bacteria, insufficient hydraulic retention time or insufficient internal reflux, and the like, so that the total nitrogen does not reach the standard, and the total nitrogen index enters the standard along with the continuous improvement of the wastewater discharge standard, and the requirement is increasingly strict, and the effective removal of the nitrate and nitrite becomes a problem to be solved urgently.
A conventional denitrifying bacterium is a bacterium that causes denitrification. Heterotrophic, facultative anaerobic bacteria such as denitrifying bacilli, Sterlla, Fluoropolar Aerobacter, and the like are most common. Bacteria that produce nitrous oxide and nitrogen by respiratory metabolism under anaerobic conditions, with nitrate or nitrogen dioxide as the final electron acceptor instead of oxygen. Because of the strict requirement on oxygen, anaerobic denitrifying bacteria are one of the main influencing factors of low denitrification efficiency. In the 80's of the 20 th century, Robertson et al reported the presence of aerobic denitrifying bacteria and aerobic denitrifying enzyme systems and confirmed that S.pan-cultured during the growth process, O.sub.2And NO3When present together, the growth rate is higher than when both are present alone. More and more studies have demonstrated the presence of bacterial aerobic denitrification and have found some bacteria with higher denitrification rates under aerobic conditions. Meanwhile, the existence of halophilic (salt-tolerant) microorganisms provides possibility for biological denitrification treatment of high-salt wastewater.
In recent years, different microbial strains of different species are screened, and certain achievements are achieved in the aspect of salt-tolerant denitrification wastewater treatment. For example, chinese published patent document CN 102703350B discloses a Bacillus altitudinis (Bacillus altitudinis) with high salt and heterotrophic nitrification-aerobic denitrification and phosphorus removal functions, which can utilize organic carbon as the sole carbon source and ammonia nitrogen as the sole nitrogen source for metabolism, and directly convert ammonia nitrogen into gas products by the heterotrophic nitrification-aerobic denitrification, thereby achieving the purpose of nitrogen removal. Chinese published patent documents CN 102747014A and CN 102703349A respectively disclose halobacter salina strains and brevibacterium parvum strains capable of biological denitrification under high salt condition and application thereof, wherein both strains can perform biological denitrification in nitrogen-containing wastewater with dissolved oxygen of 2-6 mg/L. However, the research on denitrification and denitrification under the high-salt condition is less, and the research on microbial strains for denitrifying and removing nitrate nitrogen and nitrite nitrogen in the high-salt wastewater environment is not reported in documents.
Disclosure of Invention
The invention provides a preparation method of a high-density salt-tolerant denitrifying bacteria agent suitable for wastewater treatment, aiming at the defects that the effluent nitrate and nitrite at the rear end of a saline wastewater biological denitrification system are high, the total nitrogen does not reach the standard and the like. The microbial inoculum can effectively degrade nitrate nitrogen and nitrite nitrogen in high-salinity wastewater under aerobic conditions, and ensure that the total nitrogen in effluent reaches the standard and is discharged.
The technical problem to be solved by the present invention is achieved by the following technical means. The invention relates to a preparation method of a high-density salt-tolerant denitrifying bacteria agent suitable for wastewater treatment, which is characterized by comprising the following steps: the preparation method comprises the steps of inoculating salt-tolerant denitrifying bacteria in a reactor added with biological fillers, and preparing the high-density salt-tolerant denitrifying bacteria agent according to an inoculation, enrichment culture and actual wastewater elutriation repeated circulation operation mode.
The preparation method specifically comprises the following process steps:
(1) an inoculation stage: inoculating the salt-tolerant denitrifying bacteria seed liquid into an SBR reactor added with biological fillers, wherein the concentration of thallus suspended matters is 100-500 mg/L;
(2) an enrichment culture stage: after the salt-tolerant denitrifying bacteria in the reactor gradually adapt to the surrounding environment, the salt concentration (calculated by NaCl) is maintained to be 1-10%, proper Dissolved Oxygen (DO) and temperature (T) are controlled, nutrient substances with certain concentration are added, the salt-tolerant denitrifying bacteria can grow rapidly, and the alkalinity generated by denitrification reaction is neutralized by adding acid liquor, so that the pH value of the system is maintained to be 6.5-8.5. During the period, the thallus suspended substance is attached to or grows on the surface of the biological filler to form a microorganism aggregate, and the microorganism aggregate is intercepted by the filler, so that the biomass in the system can reach 7000-8000 mg/L:
(3) actual wastewater elutriation stage. Adding the actual salt-containing chemical wastewater and initial NOx-N inlet water load of 0.05kgNOx-N/m3D, NO in effluentx-N-When the concentration is less than or equal to 1.0mg/L, the concentration is increased in steps0.2~0.4kgNOx-N/m3D water inflow load until final water inflow NOxA load of-N of 1.0 to 2.0kgNOx-N/m3D. In this stage, the microorganisms which can not be subjected to denitrification and have poor fixity are elutriated, and the high-density salt-resistant denitrifying bacteria agent is obtained. Said NOxN is mainly nitrate nitrogen and nitrite nitrogen. X is 2 or 3.
Biological fillers include, but are not limited to: suspended fillers, soft fillers, combined fillers, braided fillers or carbon fiber fillers, and the like.
The salt-tolerant denitrifying bacteria are derived from but not limited to Rhodococcus erythropolis (disclosed in the patent publication No. CN 104611279A) ((R))Rhodococcus erythropolis) LH-N13, and the preservation number of the strain is CGMCC number 6974.
The nutrient substance mainly comprises NaNO2（0.5~10g/L）、NaNO3（0.5~10g/L）、KH2PO4Or K2HPO4（0.5~10g/L）、MgCl2（0.5~10g/L）、 Na2CO30.5-10 g/L, glucose or methanol 0.5-10 g/L, NaCl (mass fraction less than or equal to 10 percent), and trace element liquid. Wherein the microelement liquid comprises the following components: CoCl2·6H2O（50~150mg/L）、CaCl2（50~150mg/L）、CuSO4·5H2O（10~50mg/L）、ZnSO4·7H2O（50~150mg/L）、FeSO4·7H2O（50~150mg/L）、MnSO4·2H2O（50~150mg/L）。
In the enrichment culture stage, the Dissolved Oxygen (DO) is controlled to be 0-3.5 mg/L, and the temperature (T) is controlled to be 10-40 ℃.
In the actual wastewater elutriation stage, the salt concentration (calculated by NaCl) in the actual salt-containing chemical wastewater is 1% -10%. The actual salt-containing chemical wastewater is any salt-containing chemical wastewater in the prior art.
The preparation method of the microbial inoculum is particularly suitable for treating the tail end of a salt-containing wastewater biochemical system, and ensures that the total nitrogen of the effluent reaches the standard and is discharged.
Compared with the prior art, the invention has the beneficial effects that: the microbial inoculum is resistant to high osmotic pressure and high salinity environment, the denitrification effect of the microbial inoculum is not influenced by 1-10% of salt concentration, nitrate nitrogen and nitrite nitrogen in salt-containing wastewater can be effectively removed, synchronous nitrification and denitrification of the high-salt nitrogen-containing wastewater are realized, and the problem that total nitrogen is difficult to reach the standard under the high-salt condition can be solved. The high-density salt-tolerant denitrifying bacteria agent prepared by the invention can be used for biological treatment of high-salt ammonia nitrogen-containing wastewater such as food processing, biological brewing, pesticides, leather and the like.
Detailed Description
The present invention will be further described with reference to the following examples, but the present invention is not limited to the following examples.
Example 1, a method for preparing a high-density salt-tolerant denitrifying bacteria agent suitable for wastewater treatment, the method comprising inoculating salt-tolerant denitrifying bacteria in a reactor with biological filler, and preparing the high-density salt-tolerant denitrifying bacteria agent according to the repeated and cyclic operation mode of inoculation, enrichment culture and actual wastewater elutriation.
Embodiment 2, the preparation method of the high-density salt-tolerant denitrifying bacteria agent suitable for wastewater treatment described in embodiment 1 specifically includes the following steps:
(1) an inoculation stage: inoculating the salt-tolerant denitrifying bacteria seed liquid into an SBR reactor added with biological fillers, wherein the concentration of thallus suspended matters is 100 mg/L;
(2) an enrichment culture stage: after the salt-tolerant denitrifying bacteria in the reactor gradually adapt to the surrounding environment, the salt concentration counted by NaCl is maintained to be 1-5%, proper dissolved oxygen and temperature are controlled, nutrient substances with certain concentration are added, the salt-tolerant denitrifying bacteria grow rapidly, the alkalinity generated by denitrification reaction is neutralized by adding acid liquor, and the pH value of the system is maintained to be 6.5-7.5; during the period, the thallus suspended substance is attached to or grows on the surface of the biological filler to form a microorganism aggregate, and the microorganism aggregate is intercepted by the filler, so that the biomass in the system can reach 7000mg/L finally;
(3) actual wastewater elutriation stage: adding the actual salt-containing chemical wastewater and initial NOx-N inlet water load of 0.05kgNOx-N/m3D, NO in effluentx-N-When the concentration is less than or equal to 1.0mg/L, the concentration is increased by 0.2-0.4 kgNO in stepsx-N/m3D ofWater inlet load until final water inlet NOxA load of-N of 1.0 to 2.0kgNOx-N/m3D; elutriating microorganisms which cannot be subjected to denitrification and have poor fixity to obtain a high-density salt-resistant denitrifying bacteria agent; said NOxN mainly refers to nitrate nitrogen and nitrite nitrogen, and X ═ 2 or 3.
Biological fillers include, but are not limited to: suspended fillers, soft fillers, combined fillers, braided fillers or carbon fiber fillers. The salt-tolerant denitrifying bacteria are Rhodococcus erythropolis (R.erythropolis)Rhodococcus erythropolis) LH-N13, and the preservation number of the strain is CGMCC number 6974. The nutrient mainly comprises 0.5-10 g/L NaNO2、0.5~10g/L NaNO3、0.5~10g/L KH2PO4Or K2HPO4，0.5~10g/L MgCl2， 0.5~10g/L Na2CO30.5-10 g/L glucose or methanol, NaCl with the mass fraction not more than 10% and trace element liquid. The trace element liquid comprises the following components: 50-150 mg/L CoCl2·6H2O、50~150mg/L CaCl2、10~50mg/L CuSO4·5H2O、50~150mg/L ZnSO4·7H2O、50~150mg/L FeSO4·7H2O、50~150mg/L MnSO4·2H2And O. In the enrichment culture stage, the dissolved oxygen is controlled to be 0.1-1.5 mg/L, and the temperature is controlled to be 10-20 ℃. In the actual wastewater elutriation stage, the salt concentration in the actual salt-containing chemical wastewater is 1% -10% calculated as NaCl.
Embodiment 3, the preparation method of the high-density salt-tolerant denitrifying bacteria agent suitable for wastewater treatment described in embodiment 1 includes the following steps:
(1) an inoculation stage: inoculating the salt-tolerant denitrifying bacteria seed liquid into an SBR reactor added with biological fillers, wherein the concentration of thallus suspended matters is 500 mg/L;
(2) an enrichment culture stage: after the salt-tolerant denitrifying bacteria in the reactor gradually adapt to the surrounding environment, the salt concentration counted by NaCl is maintained to be 5-10%, proper dissolved oxygen and temperature are controlled, nutrient substances with certain concentration are added, the salt-tolerant denitrifying bacteria grow rapidly, the alkalinity generated by denitrification reaction is neutralized by adding acid liquor, and the pH value of the system is maintained to be 7.5-8.5; during the period, the thallus suspended substance is attached to or grows on the surface of the biological filler to form a microorganism aggregate, and the microorganism aggregate is intercepted by the filler, so that the biomass in the system can reach 8000mg/L finally;
(3) actual wastewater elutriation stage: adding the actual salt-containing chemical wastewater and initial NOx-N inlet water load of 0.05kgNOx-N/m3D, NO in effluentx-N-When the concentration is less than or equal to 1.0mg/L, the concentration is increased by 0.2-0.4 kgNO in stepsx-N/m3D water inflow load until final water inflow NOxA load of-N of 1.0 to 2.0kgNOx-N/m3D; elutriating microorganisms which cannot be subjected to denitrification and have poor fixity to obtain a high-density salt-resistant denitrifying bacteria agent; said NOxN mainly refers to nitrate nitrogen and nitrite nitrogen, and X ═ 2 or 3.
Biological fillers include, but are not limited to: suspended fillers, soft fillers, combined fillers, braided fillers or carbon fiber fillers. The salt-tolerant denitrifying bacteria are Rhodococcus erythropolis (R.erythropolis)Rhodococcus erythropolis) LH-N13, and the preservation number of the strain is CGMCC number 6974. The nutrient mainly comprises 0.5-10 g/L NaNO2、0.5~10g/L NaNO3、0.5~10g/L KH2PO4Or K2HPO4，0.5~10g/L MgCl2， 0.5~10g/L Na2CO30.5-10 g/L glucose or methanol, NaCl with the mass fraction not more than 10% and trace element liquid. The trace element liquid comprises the following components: 50-150 mg/L CoCl2·6H2O、50~150mg/L CaCl2、10~50mg/L CuSO4·5H2O、50~150mg/L ZnSO4·7H2O、50~150mg/L FeSO4·7H2O、50~150mg/L MnSO4·2H2And O. In the enrichment culture stage, the dissolved oxygen is controlled to be 1.5-3.5 mg/L, and the temperature is controlled to be 20-35 ℃. In the actual wastewater elutriation stage, the salt concentration in the actual salt-containing chemical wastewater is 1% -10% calculated as NaCl.
Embodiment 4, the preparation method of the high-density salt-tolerant denitrifying bacteria agent suitable for wastewater treatment described in embodiment 1 specifically includes the following steps:
(1) an inoculation stage: inoculating the salt-tolerant denitrifying bacteria seed liquid into an SBR reactor added with biological fillers, wherein the concentration of thallus suspended matters is 300 mg/L;
(2) an enrichment culture stage: after the salt-tolerant denitrifying bacteria in the reactor gradually adapt to the surrounding environment, the salt concentration counted by NaCl is maintained to be 5-8%, proper dissolved oxygen and temperature are controlled, nutrient substances with certain concentration are added, the salt-tolerant denitrifying bacteria grow rapidly, the alkalinity generated by denitrification reaction is neutralized by adding acid liquor, and the pH value of the system is maintained to be 7.5-8.0; during the period, the thallus suspended substance is attached to or grows on the surface of the biological filler to form a microorganism aggregate, and the microorganism aggregate is intercepted by the filler, so that the biomass in the system can reach 7500mg/L finally;
(3) actual wastewater elutriation stage: adding the actual salt-containing chemical wastewater and initial NOx-N inlet water load of 0.05kgNOx-N/m3D, NO in effluentx-N-When the concentration is less than or equal to 1.0mg/L, the concentration is increased by 0.2-0.4 kgNO in stepsx-N/m3D water inflow load until final water inflow NOxA load of-N of 1.0 to 2.0kgNOx-N/m3D; elutriating microorganisms which cannot be subjected to denitrification and have poor fixity to obtain a high-density salt-resistant denitrifying bacteria agent; said NOxN mainly refers to nitrate nitrogen and nitrite nitrogen, and X ═ 2 or 3.
Biological fillers include, but are not limited to: suspended fillers, soft fillers, combined fillers, braided fillers or carbon fiber fillers. The salt-tolerant denitrifying bacteria are Rhodococcus erythropolis (R.erythropolis)Rhodococcus erythropolis) LH-N13, and the preservation number of the strain is CGMCC number 6974. The nutrient substance mainly comprises 5g/L NaNO2、10g/L NaNO3、3g/L KH2PO4Or K2HPO4，4g/L MgCl2， 6g/L Na2CO35g/L glucose or methanol, NaCl with the mass fraction less than or equal to 10 percent, and trace element liquid. The trace element liquid comprises the following components: 100mg/L CoCl2·6H2O、150mg/L CaCl2、30mg/L CuSO4·5H2O、100mg/L ZnSO4·7H2O、120mg/L FeSO4·7H2O、1100mg/L MnSO4·2H2And O. In the enrichment culture stage, dissolvingOxygen was controlled at 2.5mg/L and temperature was controlled at 25 ℃. In the actual elutriation stage of the wastewater, the salt concentration in the actual salt-containing chemical wastewater is 10% calculated by NaCl.

Claims (4)

1. A preparation method of a high-density salt-tolerant denitrifying bacteria agent suitable for wastewater treatment is characterized by comprising the following steps: the preparation method comprises the steps of inoculating salt-tolerant denitrifying bacteria in a reactor added with biological fillers, and preparing the high-density salt-tolerant denitrifying bacteria agent according to an inoculation, enrichment culture and actual wastewater elutriation repeated circulation operation mode;
the method specifically comprises the following steps:
(1) an inoculation stage: inoculating the salt-tolerant denitrifying bacteria seed liquid into an SBR reactor added with biological fillers, wherein the concentration of thallus suspended matters is 100-500 mg/L;
(2) an enrichment culture stage: after the salt-tolerant denitrifying bacteria in the reactor gradually adapt to the surrounding environment, the salt concentration counted by NaCl is maintained to be 1-10%, proper dissolved oxygen and temperature are controlled, nutrient substances with certain concentration are added, the salt-tolerant denitrifying bacteria grow rapidly, the alkalinity generated by denitrification reaction is neutralized by adding acid liquor, and the pH value of the system is maintained to be 6.5-8.5; during the period, the thallus suspended matters are attached to or grow on the surface of the biological filler to form a microorganism aggregate, and the microorganism aggregate is intercepted by the filler, so that the biomass in the system can finally reach 7000-8000 mg/L;
in the enrichment culture stage, the dissolved oxygen is controlled to be 0-3.5 mg/L, and the temperature is controlled to be 10-40 ℃;
(3) actual wastewater elutriation stage: adding the actual salt-containing chemical wastewater and initial NOx-N inlet water load of 0.05kgNOx-N/m3D, NO in effluentx-N-When the concentration is less than or equal to 1.0mg/L, the concentration is increased by 0.2-0.4 kgNO in stepsx-N/m3D water inflow load until final water inflow NOxA load of-N of 1.0 to 2.0kgNOx-N/m3D; elutriating microorganisms which cannot be subjected to denitrification and have poor fixity to obtain a high-density salt-resistant denitrifying bacteria agent; said NOxN mainly refers to nitrate and nitrite nitrogen, X ═ 2 or 3; in the actual elutriation stage of the wastewater, the salt concentration in the actual salt-containing chemical wastewater is NaCl is 1% -10%;
the salt-tolerant denitrifying bacteria are Rhodococcus erythropolis (R.erythropolis)Rhodococcus erythropolis) LH-N13, and the preservation number of the strain is CGMCC number 6974.
2. The preparation method of the high-density salt-tolerant denitrifying bacteria agent suitable for wastewater treatment according to claim 1, wherein the preparation method comprises the following steps: biological fillers include, but are not limited to: suspended fillers, soft fillers, combined fillers, braided fillers or carbon fiber fillers.
3. The preparation method of the high-density salt-tolerant denitrifying bacteria agent suitable for wastewater treatment according to claim 1, wherein the preparation method comprises the following steps: the nutrient mainly comprises 0.5-10 g/L NaNO2、0.5~10g/L NaNO3、0.5~10g/L KH2PO4Or K2HPO4，0.5~10g/L MgCl2， 0.5~10g/L Na2CO30.5-10 g/L glucose or methanol, NaCl with the mass fraction not more than 10% and trace element liquid.
4. The preparation method of the high-density salt-tolerant denitrifying bacteria agent suitable for wastewater treatment according to claim 3, wherein the preparation method comprises the following steps: the trace element liquid comprises the following components: 50-150 mg/L CoCl2·6H2O、50~150mg/L CaCl2、10~50mg/L CuSO4·5H2O、50~150mg/L ZnSO4·7H2O、50~150mg/L FeSO4·7H2O、50~150mg/L MnSO4·2H2O。
CN201710051575.XA 2017-01-23 2017-01-23 Preparation method of high-density salt-tolerant denitrifying bacteria agent suitable for wastewater treatment Active CN106635923B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710051575.XA CN106635923B (en) 2017-01-23 2017-01-23 Preparation method of high-density salt-tolerant denitrifying bacteria agent suitable for wastewater treatment

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710051575.XA CN106635923B (en) 2017-01-23 2017-01-23 Preparation method of high-density salt-tolerant denitrifying bacteria agent suitable for wastewater treatment

Publications (2)

Publication Number Publication Date
CN106635923A CN106635923A (en) 2017-05-10
CN106635923B true CN106635923B (en) 2021-06-29

Family

ID=58842038

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710051575.XA Active CN106635923B (en) 2017-01-23 2017-01-23 Preparation method of high-density salt-tolerant denitrifying bacteria agent suitable for wastewater treatment

Country Status (1)

Country Link
CN (1) CN106635923B (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109439571A (en) * 2018-10-31 2019-03-08 广州小众环保科技有限公司 A kind of ammonia nitrogen removal microbial inoculum
CN110092469A (en) * 2019-05-14 2019-08-06 清华大学 A kind of organic liquid waste/high concentration nitrate waste liquid collaboration denitrogenation removes the diphasic anaerobic processing method and processing device of organic carbon
CN111943361B (en) * 2019-05-17 2023-11-10 华东理工大学 Treatment system and method for degrading fracturing flowback fluid organic matters by salt-tolerant bacteria
CN111117913B (en) * 2019-12-28 2021-07-30 北京翰祺环境技术有限公司 High-salt-resistance high-COD wastewater degrading bacterial strain, culture method, bacterial liquid and application
CN114249432B (en) * 2020-09-24 2023-03-21 中蓝连海设计研究院有限公司 Biological promoter and application thereof
CN112408602A (en) * 2020-11-27 2021-02-26 江苏蓝必盛化工环保股份有限公司 Salt-tolerant denitrification technology in high-salt pesticide chemical wastewater
CN117303602B (en) * 2023-10-10 2024-04-16 中国海洋大学 Method for purifying nitrate nitrogen in cultivation wastewater by pyrite biological filter

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102382767A (en) * 2010-08-31 2012-03-21 中国石油化工股份有限公司 Enrichment culture method of salt-tolerant nitrifying bacterium communities
CN104726376A (en) * 2015-03-27 2015-06-24 中蓝连海设计研究院 Continuous large-scale culture method for high-concentration salt-resistant nitrifying bacteria
CN106495314A (en) * 2016-12-26 2017-03-15 中蓝连海设计研究院 Circulation wall film method and denitrification reactor in aerobic salt tolerant denitrifying bacterium

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102382767A (en) * 2010-08-31 2012-03-21 中国石油化工股份有限公司 Enrichment culture method of salt-tolerant nitrifying bacterium communities
CN104726376A (en) * 2015-03-27 2015-06-24 中蓝连海设计研究院 Continuous large-scale culture method for high-concentration salt-resistant nitrifying bacteria
CN106495314A (en) * 2016-12-26 2017-03-15 中蓝连海设计研究院 Circulation wall film method and denitrification reactor in aerobic salt tolerant denitrifying bacterium

Also Published As

Publication number Publication date
CN106635923A (en) 2017-05-10

Similar Documents

Publication Publication Date Title
CN106635923B (en) Preparation method of high-density salt-tolerant denitrifying bacteria agent suitable for wastewater treatment
CN104673738B (en) A kind of domestication of heterotrophic nitrification aerobic denitrifying bacteria and screening technique
CN101434907B (en) Microbial preparation for processing refuse leachate and preparation
CN102382767A (en) Enrichment culture method of salt-tolerant nitrifying bacterium communities
CN111117912B (en) Salt-tolerant denitrifying bacteria strain, salt-tolerant denitrifying bacteria liquid, screening method and application
CN106745713B (en) Rapid starting method of anaerobic ammonia oxidation reactor
CN103589669A (en) Salt-resistant denitriding composite inoculant, and preparation method and use method thereof
CN102952765A (en) Enrichment culture method of salt-tolerant efficient nitrosobacteria
CN108117221A (en) A kind of processing method of reverse osmosis concentrated water
CN106635947B (en) Asynchronous domestication culture method for salt-tolerant denitrification microbial inoculum
CN104611279A (en) Rhodococcus erythropolis LH-N13 as well as microbial agent and use thereof
Liu et al. Isolation and characterization of a heterotrophic nitrifier from coke plant wastewater
CN109161513B (en) Sphingobacterium and application thereof
CN106554076B (en) Biological treatment method of ammonia-containing wastewater
CN117143755A (en) Pseudomonas stutzeri strain and application thereof
CN114031189B (en) Method for treating sewage with low carbon-nitrogen ratio
CN115094013A (en) Stenotrophomonas rhizophila, microbial inoculum and application of stenotrophomonas rhizophila and microbial inoculum in wastewater treatment
CN112358041B (en) Granular sludge culture method for synchronous denitrification and methane production and COD removal
CN106630173B (en) A kind of biochemical processing method of epoxychloropropane waste water
CN111099722B (en) Composition for promoting denitrification and application thereof
CN109880784B (en) Composition for promoting denitrification of denitrifying microorganisms and application thereof
CN105624054A (en) Enrichment culture method for salt-tolerant nitrifying bacteria colonies
CN106554928B (en) Enrichment culture method of heterotrophic nitrifying bacteria
CN116354506B (en) Method for realizing heterotrophic nitrification-aerobic denitrification high-efficiency denitrification through stress of high-concentration quorum sensing inhibitor
CN114684924B (en) Denitrification treatment method for salt-containing wastewater

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
CB02 Change of applicant information
CB02 Change of applicant information

Address after: 222000 Chaoyang West Road, Haizhou District, Lianyungang, Jiangsu 51

Applicant after: CHINA BLUESTAR LEHIGH ENGINEERING Corp.

Address before: 222000 Chaoyang West Road, Haizhou District, Lianyungang, Jiangsu 51

Applicant before: CHINA BLUESTAR LEHIGH ENGINEERING Corp.

GR01 Patent grant
GR01 Patent grant