CN111170450A - Pulse type domestication method of salt-tolerant anaerobic ammonium oxidation sludge - Google Patents
Pulse type domestication method of salt-tolerant anaerobic ammonium oxidation sludge Download PDFInfo
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- CN111170450A CN111170450A CN202010038757.5A CN202010038757A CN111170450A CN 111170450 A CN111170450 A CN 111170450A CN 202010038757 A CN202010038757 A CN 202010038757A CN 111170450 A CN111170450 A CN 111170450A
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
- C02F3/2813—Anaerobic digestion processes using anaerobic contact processes
Abstract
The invention discloses a pulse type domestication method of salt-tolerant anaerobic ammonium oxidation sludge, which comprises the following steps: (1) inoculating anaerobic ammonium oxidation granular sludge in a reactor, pumping inlet water, and NH in the inlet water4 +-N and NO2 ‑The mass concentration ratio of-N is 1:1, and the operation is carried out to be stable under the conditions of anaerobic property, light shielding and 30-36 ℃; (2) adding salt ions into the intake water, changing the concentration of the salt ions by stages by adopting a pulse method, selecting and enriching the salt-tolerant anaerobic ammonium oxidation bacteria, operating each domestication stage for the same time, taking the step (1) as a first domestication stage, wherein the total number of the domestication stages is not less than 5, the concentration of the salt ions in the second domestication stage is 0.7-1.5 times of the concentration of the anammox activity semi-inhibitory salt ions, the concentration of the salt ions in the third domestication stage is 40-60% of the concentration of the salt ions in the second domestication stage, and gradually increasing the concentration of the salt ions in the intake water until the concentration reaches a target concentration in each subsequent domestication stage to finish the domestication of the salt-tolerant anaerobic ammonium oxidation sludge.
Description
Technical Field
The invention relates to the technical field of sludge domestication, in particular to a pulse domestication method of salt-tolerant anaerobic ammonium oxidation sludge.
Background
The discharge of nitrogen in urban wastewater and industrial wastewater is an important factor for driving water eutrophication. At the same time, many of these wastewaters are often accompanied by higher salinity. According to incomplete statistics, 5% of industrial wastewater produced in the world is high salinity wastewater. An increase in salinity causes an increase in osmotic pressure. Salinity exceeding 1% can generate osmotic stress on the microorganisms, thereby inhibiting the metabolic activity of the microorganisms. This presents a challenge to the biological treatment process of saline wastewater. In fact, saline wastewater is often treated by physical methods. Although physical methods can achieve good treatment effects, their economic benefits are unsatisfactory.
Anammox refers to a biological reaction in which ammonia is oxidized to nitrogen using nitrite as an electron acceptor under anaerobic conditions. Compared with the traditional nitrification-denitrification biological denitrification process, the anaerobic ammonia oxidation process does not need aeration and an additional carbon source while maintaining high-efficiency denitrification. This makes the anammox process considered a promising biological denitrification process. In addition, the anammox process also exhibits great potential in denitrification of high salinity wastewater. The anaerobic ammonium oxidation bacteria of CandidatusScalindua can even have salinity as high as 204g NaCl L-1And (3) survival.
At present, two strategies are often adopted to realize the denitrification of high-salinity nitrogen-containing wastewater based on an anaerobic ammonia oxidation process. One is salt tolerance acclimation of freshwater anaerobic ammonium oxidation bacteria, and the other is enrichment of marine anaerobic ammonium oxidation bacteria. Because of its wider strain source, the method of acclimatizing the salt tolerance of the freshwater anaerobic ammonium oxidation bacteria is mostly adopted in the actual operation. The salt tolerance domestication refers to a microbial culture process of slowly increasing the salinity in the sewage and continuously increasing the osmotic pressure to a desired value. Each increase in salinity means the elimination of a batch of microorganisms, which directly affects the results of acclimatization. Therefore, the acclimatization strategy is often one of the important factors determining the effect of the salt tolerance acclimatization. The stable and efficient acclimation strategy of the salt-tolerant anaerobic ammonium oxidation sludge is found to have important promoting significance for the biological denitrification process of the high-salinity nitrogen-containing wastewater.
Disclosure of Invention
Aiming at the defects in the field, the invention provides a pulse domestication method of salt-tolerant anaerobic ammonium oxidation sludge, which is used for rapidly selecting and enriching salt-tolerant anaerobic ammonium oxidation bacteria by reasonably planning the salinity level of each stage in the domestication process, so that more salt-tolerant anaerobic ammonium oxidation bacteria are successfully domesticated in the same time. The invention provides a new method and a new thought for the denitrification process of high-salinity wastewater based on an anaerobic ammonia oxidation process.
A pulse domestication method of salt-tolerant anaerobic ammonium oxidation sludge comprises the following steps:
(1) inoculating anaerobic ammonium oxidation granular sludge in a reactor, pumping inlet water, and NH in the inlet water4 +-N and NO2 -The mass concentration ratio of-N is 1:1, and the operation is carried out to be stable under the conditions of anaerobic property, light shielding and 30-36 ℃;
(2) adding salt ions into the influent water, changing the concentration of the salt ions by stages by adopting a pulse method, selecting and enriching salt-tolerant anaerobic ammonium oxidation bacteria, operating each domestication stage for the same time, taking the step (1) as a first domestication stage, wherein the total number of the domestication stages is not less than 5, the concentration of the salt ions in the second domestication stage is 0.7-1.5 times of the concentration of the anammox active semi-inhibitory salt ions, the concentration of the salt ions in the third domestication stage is 40-60% of the concentration of the salt ions in the second domestication stage, and the concentration of the salt ions in the influent water is gradually increased until the target concentration is reached in each subsequent domestication stage, so that the domestication of the salt-tolerant anaerobic ammonium oxidation sludge is completed.
In the primary salinity adjusting stage, the relatively high salt ion concentration is kept, specifically, the semi-inhibitory concentration of salt ions on the anammox activity can be referred to, the salt ion concentration is reduced in the secondary salinity adjusting stage, and then the salt ion concentration is gradually increased to the target concentration. The pulse type adding strategy is adopted to improve the salt ion concentration, so that microorganisms with low salinity tolerance can be effectively eliminated in the initial stage, and the salt-tolerant anaerobic ammonium oxidation bacteria can be rapidly selected; subsequently, the concentration of salt ions is reduced, so that the enrichment of the salt-tolerant anaerobic ammonium oxidation bacteria is facilitated.
Preferably, the reactor is an upflow anaerobic sludge blanket reactor, which is beneficial to maintaining the anaerobic state in the whole acclimation process.
Preferably, the concentration of the anaerobic ammonia oxidation granular sludge inoculated in the reactor is 10-15 g-1VSS L-1. VSS is a volatile suspended solid.
Preferably, the dominant genus in the anammox granular sludge is Candidatus Kuenenia.
Preferably, the pH of the influent water is 7.6 to 7.8, which is most suitable for culturing anammox bacteria.
Preferably, the feed water contains (NH)4)2SO4，NaNO2，NaH2PO4，CaCl2·2H2O，MgSO4·7H2O，NaHCO3A microelement I stock solution and a microelement II stock solution.
The stock solution of the trace element I consists of EDTA and FeSO4Is prepared by the following steps.
The microelement II stock solution contains: EDTA, ZnSO4·7H2O，CoCl2·6H2O，MnCl2·4H2O，CuSO4·5H2O，NaMoO4·2H2O，NiCl2·6H2O and H3BO4。
The feed water contains nutrients necessary for the growth of anammox bacteria and is suitable for the growth of anammox bacteria.
Preferably, the hydraulic retention time of the inlet water is 1.5-2.0 h.
Preferably, the salt ion concentration in the second acclimation stage is 0.9 to 1.1 times of the concentration of the half inhibitory salt ion on the anammox activity.
Preferably, the salt ion concentration of the fourth acclimation stage is the same as the salt ion concentration of the second acclimation stage.
Preferably, the salt ions are added in the form of NaCl, the target concentration not exceeding 30g L-1And the domestication failure caused by overhigh target concentration of the domestication is prevented.
Preferably, the operation time of each domestication stage is not less than 14 days, so that the domestication time is not too short, and the domestication result is not ideal.
In a preferred embodiment, the salt ions are added in the form of NaCl, and the NaCl concentration in the inlet water of different acclimation stages is 0.00g L-1、10.00g L-1、5.00g L-1、10.00g L-1、25.00g L-1. Under the acclimatization method, when the salinity reaches the acclimatized target concentration of 25.00g NaCl L-1At this time, the nitrogen removal rate NRR of the reactor reached 2.27. + -. 0.30kg Nm-3d-1Is obviously higher than 1.75 plus or minus 0.23kg N m under the conventional domestication method-3d-1。
Compared with the prior art, the invention has the main advantages that: according to the method, the salinity level of each stage in the acclimatization process is reasonably planned, and the microorganisms with low salinity tolerance are effectively eliminated in the initial stage, so that the salt-tolerant anaerobic ammonium oxidation bacteria are quickly selected and enriched, and a good acclimatization result is finally obtained.
Detailed Description
The invention will be further illustrated with reference to the following specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. The following examples are conducted under conditions not specified, usually according to conventional conditions, or according to conditions recommended by the manufacturer.
(1) An up-flow anaerobic sludge bed reactor with the effective volume of 1.5L is adopted, 0.6L of anaerobic ammonium oxidation granular sludge is inoculated, the reactor is placed in a thermostatic chamber with the temperature of 35 +/-1 ℃, the reactor is operated in an anaerobic and light-proof manner, the hydraulic retention time is 2.0h, the total nitrogen load is adjusted according to the operation condition of the reactor, and in the embodiment, the total nitrogen load is 6.72-3.36 kg Nm-3d-1。
The initial water inlet component is as follows:
wherein, the stock solution of the trace element I comprises the following components:
EDTA 5.00g L-1，
FeSO49.14g L-1。
the stock solution of the trace element II comprises the following components:
the pH of the initial influent water is 7.6 to 7.8.
(2) In the initial feed water (NH)4)2SO4With NaNO2All the concentrations of (A) and (B) are 280mg L-1After 14 days of culture under the conditions of (1), the total nitrogen removal rate of the reactor reaches 91.5%, and the specific anammox activity of the anammox sludge in the reactor is determined to be 187.9 +/-5.11 mg N gVSS-3d-1The dominant strain is Candidatus Kuenenia stuttgartiensis, which shows that the reactor is successfully started and stably operated and has the condition of salinity acclimation.
(3) In this embodiment, a pulse addition strategy is adopted to change the concentration of salt ions in stages, and the specific method is as follows:
(A) 10.00g L was added to the feed water in accordance with the half inhibitory concentration of NaCl on anammox activity-1And cultured for 14 days.
(B) The NaCl concentration in the inlet water is reduced to 5.00g L-1And cultured for 14 days.
(C) The NaCl concentration in the inlet water is increased to 10.00g L-1And cultured for 14 days.
(D) The NaCl concentration in the inlet water is increased to the target concentration of 25.00g L-1And cultured for 14 days.
(4) Setting up a contrast realThe test group is used for determining the domestication effect under different domestication methods, the domestication process of the control test group also comprises 4 salt ion concentration increasing stages, each stage is maintained for 14 days, the salinity level is regulated and controlled by adding NaCl, and the domestication target concentration is also 25.00g L-1The concentration of salt ions is increased by adopting an equal concentration gradient, namely the NaCl concentrations under different domestication stages are respectively 6.25g L-1、12.50g L-1、18.75g L-1、25.00g L-1。
(5) Increasing the salinity in the reactor to 25.00g NaCl L by adopting the salt concentration increasing strategies in (3) and (4) respectively-1After culturing for 14 days under the concentration, the NRR of each reactor and the related indexes of the sludge in the reactor are measured, and the NRR of the reactor is 2.27 +/-0.30 kg Nm under the pulse type salinity adding strategy-3d-1The specific anaerobic ammonia oxidation activity of the sludge in the reactor is 41.15 +/-5.73 mg of N gVSS-3d-1While the NRR of the control reactor under the same conditions was only 1.75. + -. 0.23kg NM-3d-1The specific anaerobic ammonia oxidation activity is 36.55 +/-9.07 mg N gVSS-3d-1。
In general, the method provided by the invention can be used for effectively eliminating the microorganisms with low salinity tolerance in the initial stage by reasonably planning the salinity level of each stage in the acclimation process, so that the salt-tolerant anaerobic ammonium oxidation bacteria can be rapidly selected and enriched, and a more excellent acclimation result is obtained compared with the conventional acclimation method.
The final NaCl concentration was 25.00g L-1The anaerobic ammonium oxidation granular sludge domesticated by the domestication method has excellent denitrification performance and is beneficial to the practical application of the anaerobic ammonium oxidation process.
Furthermore, it should be understood that various changes and modifications can be made by one skilled in the art after reading the above description of the present invention, and equivalents also fall within the scope of the invention as defined by the appended claims.
Claims (9)
1. A pulse type domestication method of salt-tolerant anaerobic ammonium oxidation sludge is characterized by comprising the following steps:
(1) inoculating anaerobic ammonium oxidation granular sludge in a reactor, pumping inlet water, and NH in the inlet water4 +-N and NO2 -The mass concentration ratio of-N is 1:1, and the operation is carried out to be stable under the conditions of anaerobic property, light shielding and 30-36 ℃;
(2) adding salt ions into the influent water, changing the concentration of the salt ions by stages by adopting a pulse method, selecting and enriching salt-tolerant anaerobic ammonium oxidation bacteria, operating each domestication stage for the same time, taking the step (1) as a first domestication stage, wherein the total number of the domestication stages is not less than 5, the concentration of the salt ions in the second domestication stage is 0.7-1.5 times of the concentration of the anammox active semi-inhibitory salt ions, the concentration of the salt ions in the third domestication stage is 40-60% of the concentration of the salt ions in the second domestication stage, and the concentration of the salt ions in the influent water is gradually increased until the target concentration is reached in each subsequent domestication stage, so that the domestication of the salt-tolerant anaerobic ammonium oxidation sludge is completed.
2. The pulsed acclimation method of the salt-tolerant anammox sludge according to claim 1, wherein the reactor is an upflow anaerobic sludge bed reactor.
3. The pulse acclimation method for the salt-tolerant anammox sludge according to claim 1, wherein the concentration of the anammox granular sludge inoculated in the reactor is 10-15 g-1VSS L-1。
4. The pulsed acclimation method of the salt-tolerant anammox sludge according to claim 1, wherein the dominant bacteria in the anammox granular sludge is Candidatus Kuenenia.
5. The pulse acclimation method for the salt-tolerant anammox sludge according to claim 1, wherein the pH of the influent water is 7.6-7.8, and the hydraulic retention time is 1.5-2.0 h.
6. The pulsed acclimation method for the salt-tolerant anammox sludge according to claim 1, wherein the salt ion concentration in the second acclimation stage is 0.9-1.1 times of the concentration of the anammox active semi-inhibitory salt ions.
7. The pulsed acclimation method of salt-tolerant anammox sludge according to claim 1 or 6, wherein the salt ion concentration of the fourth acclimation stage is the same as the salt ion concentration of the second acclimation stage.
8. The method for pulsed acclimation of salt-tolerant anammox sludge according to claim 1, wherein the salt ions are added in the form of NaCl, and the target concentration is not more than 30g L-1。
9. The pulsed acclimation method for salt-tolerant anammox sludge according to claim 1, wherein each acclimation period is operated for not less than 14 days.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114873743A (en) * | 2022-06-13 | 2022-08-09 | 海南大学 | Device and method for realizing rapid enrichment culture of marine anaerobic ammonium oxidation bacteria |
| JP7345079B1 (en) | 2022-06-21 | 2023-09-14 | 鹿島建設株式会社 | Mixed microorganisms and wastewater treatment method |
| WO2023248512A1 (en) * | 2022-06-21 | 2023-12-28 | 鹿島建設株式会社 | Mixed microorganisms and wastewater treatment method |
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| CN105254004A (en) * | 2015-09-29 | 2016-01-20 | 杭州师范大学 | Culture method of sulfide tolerant anaerobic ammonium oxidation granular sludge culture material |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114873743A (en) * | 2022-06-13 | 2022-08-09 | 海南大学 | Device and method for realizing rapid enrichment culture of marine anaerobic ammonium oxidation bacteria |
| CN114873743B (en) * | 2022-06-13 | 2023-03-10 | 海南大学 | Device and method for realizing rapid enrichment culture of marine anaerobic ammonium oxidation bacteria |
| JP7345079B1 (en) | 2022-06-21 | 2023-09-14 | 鹿島建設株式会社 | Mixed microorganisms and wastewater treatment method |
| WO2023248512A1 (en) * | 2022-06-21 | 2023-12-28 | 鹿島建設株式会社 | Mixed microorganisms and wastewater treatment method |
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Application publication date: 20200519 |