CN106630174A - Method for treating epichlorohydrin wastewater - Google Patents
Method for treating epichlorohydrin wastewater Download PDFInfo
- Publication number
- CN106630174A CN106630174A CN201510737854.2A CN201510737854A CN106630174A CN 106630174 A CN106630174 A CN 106630174A CN 201510737854 A CN201510737854 A CN 201510737854A CN 106630174 A CN106630174 A CN 106630174A
- Authority
- CN
- China
- Prior art keywords
- culture
- fstb
- cod
- calcium ion
- waste water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/36—Organic compounds containing halogen
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/38—Pseudomonas
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention relates to a method for treating epichlorohydrin wastewater. The epichlorohydrin wastewater is treated by a biochemical process, in a treatment process, a mode of increasing the concentrations of COD and calcium ions in water step by step is adopted, and meanwhile, halotolerant bacteria are fed, are Pseudomonas stutzeri FSTB-5, and have been deposited in the China General Microbiological Culture Collection Center with the accession number of CGMCC No. 10940. The specific halotolerant bacteria are fed in the a biochemical treatment process of the epichlorohydrin wastewater, a treatment system can bear environments with high salinity and high calcium, and COD in the wastewater can be removed effectively. The method has the characteristics of simple process, high treatment efficiency, low treatment cost and the like.
Description
Technical field
The invention belongs to environment-protection wastewater processing technology field, and in particular to a kind of method of process epoxychloropropane waste water.
Background technology
Epoxychloropropane is a kind of important organic synthesis intermediate, mainly for the production of fine chemical products such as epoxy resin, synthetic glycerine, chlorohydrin rubbers.Propylene high-temperature chloridising is the classical way for industrially producing epoxychloropropane, and the sewage produced in technical process has the features such as water yield is big, salinity is high, calcium ion concentration is high, and independent intractability is big, costly.Presently relevant enterprise frequently results in biochemical treatment system doped calcium substantially using biochemical processing process after mixing with the waste water of other process units, causes inorganic constituents in activated sludge to raise, the phenomenon such as Sludge Property deterioration.
With increasingly strict, the subject matter for being processed into restriction industrial water saving development of epoxychloropropane waste water of environmental protection requirement, the economic benefit and social benefit of enterprise are severely impacted.Therefore the pretreatment of epoxychloropropane waste water except calcium ion, to reduce salt content very crucial.For high slat-containing wastewater can be to be processed by the way of evaporation, but the waste water water yield produced in epoxychloropropane production process is big, it is clear that using being pre-processed to waste water because relatively costly by the way of evaporating, enterprise cannot bear.External useful membrane technology processes the waste water of production of propylene oxide chloride containing calcium, but because costly use is restricted.The waste water high for calcium ions concentration, is generally in advance removed calcium ion using carbonate, and not only the removal of calcium ion is limited, and the salt ionic concentration in waste water can be caused to raise, still cannot directly using biochemical process process.
In the biochemical processing process of epoxychloropropane waste water, microorganism is the key factor for affecting good waste water treatment effect bad.Using A/O, SBR and MBR etc. more than existing handling process, operation pH value is more between 7.5-8.5, easily occurs problems with actual moving process：（1）Water treatment effect is deteriorated after bioprocess technology section operation a period of time；（2）Sludge settling property improves causes sludge closely knit, and Treatment of Sludge effect is deteriorated；（3）The calcium ion of high concentration is changed into the insoluble matter of calcium carbonate, calcium sulfate and other calcium and is attached to above microorganism, causes microorganism calcification, reduces the performance of microbiological treatment waste water.
CN200710011998.5 discloses a kind of method of the production waste water of process expoxy propane, mainly includes three steps：（1）Reacted using ammonium hydrogen carbonate and calcium chloride, generate calcium bicarbonate and ammonium chloride；（2）Calcium bicarbonate thermally decomposes to generate precipitation of calcium carbonate, water and carbon dioxide；（3）Calcium hydroxide in waste water thermally decomposes the carbon dioxide reaction for producing with calcium bicarbonate, generates precipitation of calcium carbonate and water.Although the invention can remove calcium ion, the removal of salt ion can not be solved the problems, such as.CN201110135948.4 discloses a kind of method of chlorohydrination epoxychloropropane saponification waste-water recycling, feature be by the calcium chloride transformation in saponification waste-water be winnofil, simultaneously the brine waste containing organic matter is obtained, still cannot directly using biochemical process process.
In high slat-containing wastewater process field, salt content is a key factor for affecting microbiological treatment waste water efficiency.Believe glad etc.（Biological reinforcing technology processes highly salt containing organic waste water [J]. water technology, 2008,8：66-70）Highly salt containing organic waste water is processed using biological reinforcing technology, significantly improved using the dehydrogenase activity of the biological reinforced saponin waste water biological treatment system activated sludge of Facultative Halophiles, when system tolerance chlorine ion concentration is up to 2.8%, the clearance of saponin waste water COD is 84.41%.CN201210130657.0, CN201210130644.3, CN201010536065.X and CN201210130658.5 are related to using marsh cock Salmonella（Kocuri apalustris）FSDN-A, Staphylococcus cohnis（Staphylococcus
cohnii）FSDN-C, arthrobacterium（Arthrobacter creatinolyticus）FDN-1, Shui Shi Flavobacterium（Flavobacterium mizutaii）FDN-2, Paracoccus denitrificans（Paracoccus denitrificans）DN-3 and Methylobacterium（Methylobacterium phyllosphaerae）The method that SDN-3 is carried out a biological disposal upon to the ammonia nitrogen in brine waste and catalytic cracking catalyst waste water, total nitrogen and COD.The method needs to cultivate various bacterial strains and carries out compounding and prepares salt tolerant microbial inoculum, and preparation process is complicated, and production cost is higher.
The content of the invention
For the problems referred to above, the invention provides a kind of method for processing epoxychloropropane waste water.The present invention adds specific Facultative Halophiles in epoxychloropropane wastewater biochemical processing procedure, and system for handling is resistant to high salt, high calcium environment, realizes the efficient removal of COD in waste water, with process is simple, treatment effeciency height, the low feature of processing cost.
The processing method of epoxychloropropane waste water of the present invention, including following content：Epoxychloropropane waste water is processed using biochemical process, using improving in water inlet by the way of COD and calcium ion concentration step by step in processing procedure, while adding Facultative Halophiles, the Facultative Halophiles are Pseudomonas stutzeri（Pseudomonas
stutzeri）FSTB-5, is preserved in " China Committee for Culture Collection of Microorganisms's common micro-organisms center " on June 1st, 2015, and deposit number is CGMCCNo.10940.
In the present invention, the water quality of epoxychloropropane waste water is：Calcium ion concentration is 2000-8000mg/L, preferably 2000-4000mg/L, COD（Cr methods, similarly hereinafter）For 800-2500mg/L, preferably 1000-1500mg/, total salt quantity is 0.6wt%-5.0wt%.During startup, it is 200-1000mg/L to control calcium ion concentration in water inlet, when continuous 3 batch COD clearances are up to more than 90%, improves water inlet calcium ion concentration, and 50-100mg/L is improved every time, until bringing up to calcium ion concentration for 2000-8000mg/L.
In the present invention, it is known in those skilled in the art, such as A/O that biochemical process processes epoxychloropropane waste water（Anaerobic-aerobic process）、SBR（Sequencing batch active sludge）, MBR (membrane biological process) etc., preferably use membrane biological process.Treatment conditions are：Temperature is 20-40 DEG C, and pH is 6.0-9.0, and dissolved oxygen is 1-5mg/L.
In the present invention, described Pseudomonas stutzeri（Pseudomonas stutzeri）FSTB-5 bacterial strains, are preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center（CGMCC）；Address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica；Deposit number：CGMCC No.10940；Preservation date：On June 1st, 2015.The main morphological features of the bacterial strain are：Colony colour is shallow ginger color, and it is shaft-like that bacterial strain is individual；Physiological and biochemical property is：Gram-negative, oxidase negative contacts enzyme positive, and with nitrate reduction performance, hydrolyzable utilizes several kinds of carbon source.The bacterial strain can be applicable to the efficient removal of COD in the brine waste of salt content 1.0wt%-10.0wt%, and can grow under 40 DEG C of high temperature.
In the present invention, the Pseudomonas stutzeri FSTB-5 cultural methods for adding, including the three phases such as bacterial strain activation, liquid seeds liquid culture, aeration culture, concretely comprise the following steps：
（1）Bacterial strain is activated：Pseudomonas stutzeri FSTB-5 is inoculated in the inclined-plane of FSTB solid mediums or flat board, 25-40 DEG C of culture 24-48 hour, in being then stored in 4 DEG C of refrigerators.
（2）Liquid seeds liquid culture：Prepare FSTB fluid nutrient mediums, in being sub-packed in triangular flask, sterilize and be cooled to after room temperature, the inoculation activated in picking inclined-plane or flat board under gnotobasis in triangular flask, 25-40 DEG C of culture 24-72h.The FSTB fluid nutrient mediums are：FeSO4•7H2O
25mg/L, NH4NO3
286mg/L, KCl 929mg/L, CaCl22769mg/L, NaCl 21008mg/L, beef extract 5g/L, peptone 10g/L, pH value is 5.5-8.5, preferably 6.5-8.0.FSTB solid mediums are addition 20g/L agar in liquid medium within.
（3）Aeration culture：FSTB fluid nutrient mediums are added in the reactor for be provided with aerator, ratio inoculation liquid seed liquor according to reactor volume than 5%-25%, pH value is controlled in 5.0-8.5, aeration culture 48-96 hours, periodic feed supplement and discharge operation are carried out afterwards, withdrawal rate accounts for the 5%-90% of reactor volume, feed supplement amount accounts for the 5%-90% of reactor volume, also a small amount of carbon source can be added, nitrogen source and microelement substance, culture 24-48 hours are 1 cultivation cycle, discharge the nutrient solution of corresponding volume according to aforementioned proportion afterwards, thus the dense bacterium solution product containing high concentration Pseudomonas stutzeri is obtained.
In the present invention, the dense bacterium solution product that above-mentioned culture is obtained is added in wastewater treatment system according to the 0.01%-1% of handled wastewater volume per hour, preferably 0.1%-0.5%.
In the present invention, in the culture of long term open formula or wastewater treatment, still might have other a small amount of interference strain growths, to prevent from disturbing bacterium continuation flourish to become dominant bacteria, can be with taken at regular intervals culture bacterium solution, during the flat board containing FSTB solid mediums is applied to after dilution, bacterium colony to growing is counted and is counted the percentage of bacterium colony similar to FSTB-5, when percent similarity it is close 50% when, add lincomycin, minocycline, Rifamycin Sodium, troleandomycin, vancomycin, one or more in AZT and acidum nalidixicum etc., concentration is added for 0.01-100mg/L, persistently add 2-7 cultivation cycle, the microorganism of floating is discharged in draining discharge, recover normal open culture operating process afterwards.
Compared with prior art, the invention has the advantages that：
（1）The present invention adds specific Facultative Halophiles in the processing procedure of epoxychloropropane waste water, so that biochemical treatment system is resistant to high salt, high calcium environment, realize the efficient removal of COD in waste water, calcium ion need not thoroughly be removed, waste water just can directly carry out biochemical treatment, with process is simple, treatment effeciency height, the low feature of processing cost.
（2）Pseudomonas stutzeri FSTB-5 can be applied not only to the efficient removal of COD in brine waste, can particularly tolerate high calcium environment, be especially suitable for the biochemical treatment of epoxychloropropane waste water, little with dosage, the low advantage of processing cost.
Specific embodiment
The effect of the present invention is further illustrated with reference to embodiment, but is not so limited the present invention.
The cultural method of the Pseudomonas stutzeri FSTB-5 that the embodiment of the present invention is adopted includes bacterial strain activation, liquid seeds liquid culture, aeration culture, and detailed process is as follows：
（1）Bacterial strain is activated：Pseudomonas stutzeri FSTB-5 is inoculated in the flat board containing FSTB solid mediums, is cultivated 48 hours under 35 DEG C of environment, be then stored in stand-by in 4 DEG C of refrigerators.The FSTB solid mediums are：FeSO4•7H2O
25mg/L, NH4NO3286mg/L, KCl 929mg/L, CaCl2
2769mg/L, NaCl 21008mg/L, beef extract 5g/L, peptone 10g/L, agar 20g/L, pH value 7.8.
（2）Liquid seeds liquid culture：FSTB fluid nutrient mediums are prepared, in being sub-packed in triangular flask, is sterilized and is cooled to after room temperature, cultivated 48 hours under the conditions of 35 DEG C in triangular flask with the inoculation after activation in oese picking flat board under gnotobasis.The FSTB fluid nutrient mediums are：FeSO4•7H2O 25mg/L, NH4NO3286mg/L, KCl 929mg/L, CaCl22769mg/L, NaCl 21008mg/L, beef extract 5g/L, peptone 10g/L, pH value 7.8.
（3）Aeration culture：Cultivated using closed reactor，It is complete that institute is required to sterilizing using reactor and various utensils，Air inlet and exhaust position need to install bacteriological filter apparatus，Nutrient solution、Acid-base modifier and trace element solution are required to be added according to sterile working code after sterilizing，Reactor need to be equipped with aerator，And can carry out into water、Acid adjustment、Alkali tune、Feed supplement and draining discharge are operated，The FSTB fluid nutrient mediums being put in the utensil after sterilizing，Ratio according to reactor volume percentage 10% is inoculated with liquid seed liquor，After opening incubation，Adopt soda acid automatic control system by medium pH value scope control in 5.0-8.0 in incubation，Aeration culture carries out afterwards periodic feed supplement and discharge operation for 72 hours，Discharge is the nutrient solution of reactor volume percentage 25%，Feed supplement is the FSTB fluid nutrient mediums of reactor volume percentage 25%，Culture 24 hours is 1 cultivation cycle，Discharge the nutrient solution of corresponding volume according to aforementioned proportion afterwards，Thus the dense bacterium solution product containing Pseudomonas stutzeri is obtained.
The dense bacterium solution of the Pseudomonas stutzeri FSTB-5 that the embodiment of the present invention is obtained using above-mentioned culture is added.
Embodiment 1
Certain enterprise produces the waste water of a large amount of calcium ions in epoxychloropropane production process, and COD concentration therein is 800mg/L, calcium ion concentration：4000mg/L, total salt quantity：1.2wt%.System for handling is to adopt sequencing batch active sludge, and treatment temperature is 30 DEG C, and pH is 7.0, and dissolved oxygen is 2-3mg/L.During startup, it is 750mg/L to control calcium ion concentration in water inlet, and COD clearances improve influent concentration up to more than 90% in continuous 3 batches 48h, and calcium ion concentration improves every time 50mg/L, until bringing up to calcium ion concentration for 4000mg/L；The dense bacterium solution product of above-mentioned cultured Facultative Halophiles is added simultaneously, and dosage is added according to the 0.1% of handled wastewater volume per hour.COD concentration is less than 3500mg/L less than 60mg/L, calcium ion concentration in waste water after treatment, not only realizes the efficient removal of COD in epoxychloropropane waste water, but also can remove part calcium ion.
Embodiment 2
Certain enterprise produces the waste water of a large amount of calcium ions in epoxychloropropane production process, and COD concentration therein is 1450mg/L, calcium ion concentration：2500mg/L, total salt quantity：2.4%.System for handling is to adopt sequencing batch active sludge, and treatment temperature is 40 DEG C, and pH is 6.0, and dissolved oxygen is 2-3mg/L.During startup, it is 250mg/L to control calcium ion concentration in water inlet, and COD clearances improve influent concentration up to more than 90% in continuous 3 batches 36h, and calcium ion concentration improves every time 80mg/L, until bringing up to calcium ion concentration for 2500mg/L；The dense bacterium solution product of above-mentioned cultured Facultative Halophiles is added simultaneously, and dosage is added according to the 0.4% of handled wastewater volume per hour.COD concentration is less than 2000mg/L less than 60mg/L, calcium ion concentration in waste water after treatment, not only realizes the efficient removal of COD in epoxychloropropane waste water, but also can remove part calcium ion.
Embodiment 3
Certain enterprise produces the waste water of a large amount of calcium ions in epoxychloropropane production process, and COD concentration therein is 2350mg/L, calcium ion concentration：3000mg/L, total salt quantity：2.8%.System for handling is membrane biological process, and treatment temperature is 35 DEG C, and pH is 7.5, and dissolved oxygen is 2-3mg/L.During startup, it is 600mg/L to control calcium ion concentration in water inlet, and COD clearances improve influent concentration up to more than 90% in continuous 3 batches 36h, and calcium ion concentration improves every time 100mg/L, until bringing up to calcium ion concentration for 3000mg/L；The dense bacterium solution product of above-mentioned cultured Facultative Halophiles is added simultaneously, and dosage is added according to the 0.4% of handled wastewater volume per hour.COD concentration is less than 2500mg/L less than 60mg/L, calcium ion concentration in waste water after treatment, not only realizes the efficient removal of COD in epoxychloropropane waste water, but also can remove part calcium ion.
Comparative example 1
Process waste water and operating condition is same as Example 1, difference is：Facultative Halophiles are not added in processing procedure.Because calcium ion concentration is too high, biochemical treatment system doped calcium, Sludge Property is caused to deteriorate, treatment effect is not good.
Claims (10)
1. it is a kind of process epoxychloropropane waste water method, it is characterised in that including following content：Epoxychloropropane waste water is processed using biochemical process, using improving in water inlet by the way of COD and calcium ion concentration step by step in processing procedure, while adding Facultative Halophiles, the Facultative Halophiles are Pseudomonas stutzeri（Pseudomonas stutzeri）FSTB-5, is preserved in " China Committee for Culture Collection of Microorganisms's common micro-organisms center " on June 1st, 2015, and deposit number is CGMCCNo.10940.
2. method according to claim 1, it is characterised in that：The water quality of epoxychloropropane waste water is：Calcium ion concentration is 2000-8000mg/L, and COD is 800-2500mg/L, and total salt quantity is 0.6wt%-5.0wt%.
3. method according to claim 1, it is characterised in that：During startup, it is 200-1000mg/L to control calcium ion concentration in water inlet, when continuous 3 batch COD clearances are up to more than 90%, improves water inlet calcium ion concentration, and 50-100mg/L is improved every time, until bringing up to calcium ion concentration for 2000-8000mg/L.
4. method according to claim 1, it is characterised in that：The biochemical process is A/O（Anaerobic-aerobic process）、SBR（Sequencing batch active sludge）Or MBR（Membrane biological process）.
5. the method according to claim 1 or 4, it is characterised in that：The treatment conditions of the biochemical process are：Temperature is 20-40 DEG C, and pH is 6.0-9.0, and dissolved oxygen is 0.5-5mg/L.
6. method according to claim 1, it is characterised in that：The main morphological features of the Pseudomonas stutzeri FSTB-5 bacterial strains are：Colony colour is shallow ginger color, and it is shaft-like that bacterial strain is individual；Physiological and biochemical property is：Gram-negative, oxidase negative contacts enzyme positive, and with nitrate reduction performance, hydrolyzable utilizes several kinds of carbon source；The bacterial strain can be applicable to the efficient removal of COD in the brine waste of salt content 1.0wt%-10wt%, and can grow under 40 DEG C of high temperature.
7. method according to claim 1, it is characterised in that：The cultural method of the Pseudomonas stutzeri FSTB-5 includes the three phases such as bacterial strain activation, liquid seeds liquid culture, aeration culture, concretely comprises the following steps：
（1）Bacterial strain is activated：Pseudomonas stutzeri FSTB-5 is inoculated in the inclined-plane of FSTB solid mediums or flat board, 25-40 DEG C of culture 24-48 hour, in being then stored in 4 DEG C of refrigerators；
（2）Liquid seeds liquid culture：Prepare FSTB fluid nutrient mediums, in being sub-packed in triangular flask, sterilize and be cooled to after room temperature, the inoculation activated in picking inclined-plane or flat board under gnotobasis in triangular flask, 25-40 DEG C of culture 24-72h；
（3）Aeration culture：FSTB fluid nutrient mediums are added in the reactor for be provided with aerator, ratio inoculation liquid seed liquor according to reactor volume than 5%-25%, pH value is controlled in 5.0-8.5, aeration culture 48-96 hours, periodic feed supplement and discharge operation are carried out afterwards, withdrawal rate accounts for the 5%-90% of reactor volume, feed supplement amount accounts for the 5%-90% of reactor volume, culture 24-48 hours are 1 cultivation cycle, discharge the nutrient solution of corresponding volume according to aforementioned proportion afterwards, thus obtain the dense bacterium solution product containing high concentration Pseudomonas stutzeri.
8. method according to claim 7, it is characterised in that：The FSTB fluid nutrient mediums are：FeSO4•7H2O
25mg/L, NH4NO3
286mg/L, KCl 929mg/L, CaCl22769mg/L, NaCl 21008mg/L, beef extract 5g/L, peptone 10g/L, pH value is 6.0-8.5；FSTB solid mediums are addition 20g/L agar in liquid medium within.
9. method according to claim 7, it is characterised in that：The dense bacterium solution product that above-mentioned culture is obtained is added in wastewater treatment system according to the 0.01%-1% of handled wastewater volume per hour.
10. the method according to claim 1 or 7, it is characterised in that：In the culture of long term open formula or wastewater treatment, to prevent from disturbing bacterium continuation flourish to become dominant bacteria, taken at regular intervals culture bacterium solution, during the flat board containing FSTB solid mediums is applied to after dilution, bacterium colony to growing is counted and is counted the percentage of bacterium colony similar to FSTB-5, when percent similarity it is close 50% when, add lincomycin, minocycline, Rifamycin Sodium, troleandomycin, vancomycin, AZT, one or more in acidum nalidixicum, concentration is added for 0.01-100mg/L, persistently add 2-7 cultivation cycle, the microorganism of floating is discharged in draining discharge, recover normal open culture operating process afterwards.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510737854.2A CN106630174A (en) | 2015-11-04 | 2015-11-04 | Method for treating epichlorohydrin wastewater |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510737854.2A CN106630174A (en) | 2015-11-04 | 2015-11-04 | Method for treating epichlorohydrin wastewater |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106630174A true CN106630174A (en) | 2017-05-10 |
Family
ID=58810294
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510737854.2A Pending CN106630174A (en) | 2015-11-04 | 2015-11-04 | Method for treating epichlorohydrin wastewater |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106630174A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111909885A (en) * | 2020-09-23 | 2020-11-10 | 苏州道源华智环保科技有限公司 | Salt-tolerant COD-reducing strain, culture method and application |
| CN115057523A (en) * | 2022-05-06 | 2022-09-16 | 湖南英韦尔特环保科技有限公司 | SBR wastewater treatment device and treatment method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102849857A (en) * | 2012-04-10 | 2013-01-02 | 南京大学 | Method for treating high-salinity high-concentration ammonia-nitrogen wastewater |
| CN103667161A (en) * | 2013-12-25 | 2014-03-26 | 天津凯英科技发展有限公司 | Pseudomonas stutzeri KY-02 and application thereof |
-
2015
- 2015-11-04 CN CN201510737854.2A patent/CN106630174A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102849857A (en) * | 2012-04-10 | 2013-01-02 | 南京大学 | Method for treating high-salinity high-concentration ammonia-nitrogen wastewater |
| CN103667161A (en) * | 2013-12-25 | 2014-03-26 | 天津凯英科技发展有限公司 | Pseudomonas stutzeri KY-02 and application thereof |
Non-Patent Citations (4)
| Title |
|---|
| 吴鹍: "高盐环氧丙烷废水物化-生化处理研究", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
| 徐秀华: "《临床医院感染学》", 31 October 1998, 湖南科学技术出版社 * |
| 杨暑伏等: "《非发酵革兰氏阴性杆菌分类鉴定》", 31 March 1995, 哈尔滨出版社 * |
| 韩北忠等: "《发酵工程》", 31 January 2013, 中国轻工业出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111909885A (en) * | 2020-09-23 | 2020-11-10 | 苏州道源华智环保科技有限公司 | Salt-tolerant COD-reducing strain, culture method and application |
| CN115057523A (en) * | 2022-05-06 | 2022-09-16 | 湖南英韦尔特环保科技有限公司 | SBR wastewater treatment device and treatment method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106635857B (en) | A kind of Pseudomonas stutzeri and its culture application | |
| CN106746159A (en) | A kind of processing method of antibiotic production wastewater | |
| CN109055282B (en) | Novel Klebsiella pneumoniae strain and separation method and application thereof | |
| CN106635861B (en) | A kind of de- COD denitrification microorganism microbial inoculum of salt tolerant and preparation method thereof | |
| WO2021077453A1 (en) | Pseudomonas stutzeri, composite microbial inoculum prepared using the pseudomonas stutzeri, and use of the composite microbial inoculum | |
| CN106635858A (en) | Paracoccus sp. and culture application thereof | |
| CN106635923B (en) | Preparation method of high-density salt-tolerant denitrifying bacteria agent suitable for wastewater treatment | |
| CN108117221B (en) | Treatment method of reverse osmosis concentrated water | |
| CN103756947A (en) | Acinetobacter sp. and preparation method of acinetobacter sp. agent | |
| CN107699513B (en) | Black and odorous water body degrading bacterium and application thereof | |
| CN101974459B (en) | Microbes capable of degrading phenol and cyanogen in coking waste water and method for treating coking waste water by using same | |
| CN106746160A (en) | A kind of vancomycin produces the processing method of waste water | |
| CN106746161A (en) | A kind of rifamycin produces the processing method of waste water | |
| CN101386822B (en) | Special effect phosphate accumulating organisms and waste water processing method using thereof | |
| CN106746156B (en) | Method for treating epichlorohydrin production wastewater | |
| CN101054242B (en) | Application of planococcus psychrotoleratus in treating sewage at low temperature | |
| CN106635855B (en) | Microbacterium and its culture application are seen in a kind of north | |
| CN106630174A (en) | Method for treating epichlorohydrin wastewater | |
| CN106630173B (en) | A kind of biochemical processing method of epoxychloropropane waste water | |
| CN115094013B (en) | Stenotrophomonas rhizophila, microbial inoculum and application of stenotrophomonas rhizophila in wastewater treatment | |
| CN106746162A (en) | A kind of processing method of cellulose ethanol production wastewater | |
| CN107487868A (en) | A kind of method that nano cupric oxide cooperates with Cr VI in water removal with bacterium | |
| CN101054241A (en) | Application of flavobacterium omnivorum in treating sewage at low temperature | |
| CN101418266A (en) | Ochrobactrum CTS-325 and culture method thereof and application thereof in reduction of hexavalent chromium | |
| CN106630172B (en) | A kind of processing method of epoxychloropropane waste water |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170510 |
|
| RJ01 | Rejection of invention patent application after publication |