CN112063568B - Heterotrophic nitrification-aerobic denitrification bacterial agent and application thereof in natural water purification - Google Patents
Heterotrophic nitrification-aerobic denitrification bacterial agent and application thereof in natural water purification Download PDFInfo
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Abstract
The invention discloses a heterotrophic nitrification-aerobic denitrification bacterial agent and application thereof in natural water body purification, wherein the heterotrophic nitrification-aerobic denitrification bacterial agent is prepared by the following method: taking OD 600 S1, S2, S3, S4, S5, and S6 of 1 ± 0.1 volume ratio 1. Experiments prove that the heterotrophic nitrification-aerobic denitrification bacterial agent provided by the invention can be used for treating NO in natural water 3 ‑ -N removal 97.46%; for NO 2 ‑ the-N removal rate was 91.14%.
Description
Technical Field
The invention belongs to the field of natural water body purification, and particularly relates to a microbial inoculum of a heterotrophic nitrification-aerobic denitrification strain and application thereof.
Background
Reports on eutrophication of urban river channels have increased significantly over the past half century. Bioremediation is one of the most popular methods for remediating aquatic environments at present because it can degrade pollutants during the metabolic process and has a certain bio-sustainability. The traditional biological denitrification process for sewage treatment comprises aerobic nitrification and anoxic denitrification processes, but the anoxic denitrification condition is difficult to meet in natural water body. Therefore, even if the ammonia nitrogen concentration in the river water can be reduced by aeration and oxygen charging measures, the total nitrogen removal is very limited due to the limitation of denitrification. In 1983, robertson et al first separated a heterotrophic nitrification-aerobic denitrification (HN-AD) strain from desulfurization and denitrification wastewater, and the strain can perform nitrification and denitrification under aerobic conditions, thus causing wide attention.
In recent years, researchers have isolated approximately 20 genera of about 100 bacteria with HN-AD metabolic characteristics from different environments. At present, most of the research is focused on the aspects of screening new strains and the denitrification capability of the strains in the culture medium. Some studies have investigated the removal performance of pollutants and the treatment effect of actual wastewater by using HN-AD strains isolated from wastewater, but almost all wastewater conditions exhibiting high denitrification efficiency have a carbon-to-nitrogen ratio (C/N) of 8 to 10. As the C/N of the river water of the urban riverway is generally lower than 6 and most of the C/N is intensively distributed between 0.5 and 2, the application of HN-AD bacteria in the aspect of river water denitrification and purification is greatly limited. Although a few documents report the purification effect of HN-AD strains on lake water in a reactor, cellulose substances such as wood chips and the like are added into the reactor as an additional supplementary carbon source to ensure the denitrification effect. Therefore, the low C/N condition in the natural water body is the difficult problem of the denitrification treatment of the surface water. Although a large number of HN-AD strains have been isolated from river water, it is difficult to use the HN-AD strains for denitrification purification of actual river water with low C/N.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a heterotrophic nitrification-aerobic denitrification bacterial agent.
The second purpose of the invention is to provide the application of the heterotrophic nitrification-aerobic denitrification bacterial agent in the purification of natural water.
The technical scheme of the invention is summarized as follows:
heterotrophic nitrification-aerobic denitrification bacteria agent is characterized in that the agent is made of Pseudomonas stutzeri with the preservation number CCUG 12913; pseudomonas pseudomonad pseudomonas accession number DSM 25830; pseudomonas, deposit number DSM 25356; bar Li Ali Pseudomonas balerarcia accession number CCUG 08492; klebsiella variicola with Klebsiella variicola deposit number CCUG 10925; and Catellibacterium terrae accession number BMZ 065747.
Preferably, pseudomonas stutzeri deposit number CCUG 12913, pseudomonas sp deposit number DSM 25830, pseudomonas pseudomonadonas sp. deposit number DSM 25356, bara Li Ali Pseudomonas balerica deposit number CCUG 08492, klebsiella variicola deposit number CCUG 10925, and Catellibacterium terrae deposit number BMZ065747 at OD 600 1 + 0.1 volume ratio is 1.
The heterotrophic nitrification-aerobic denitrification bacterial agent is applied to natural water body purification.
The invention has the advantages that:
experiments prove that the heterotrophic nitrification-aerobic denitrification bacterial agent provided by the invention can be used for treating NO in natural water 3 - -N removal 97.46%; for NO 2 - the-N removal rate was 91.14%.
Drawings
FIG. 1 shows the denitrification performance of 6 strains of heterotrophic nitrification-aerobic denitrification bacteria in a culture medium.
FIG. 2 shows the denitrification effect of the heterotrophic nitrification-aerobic denitrification bacterial agent prepared in example 2 on actual urban river water. "C" represents a control group not inoculated with the inoculum.
Detailed Description
The bacteria related to the invention:
pseudomonas stutzeri deposit number CCUG 12913 (S1); pseudomonas sp. deposit number DSM 25830 (abbreviated as S2); pseudomonas sp. deposit number DSM 25356 (abbreviated as S3); bar Li Ali Pseudomonas balerarcia deposit number CCUG 08492 (S4 for short); klebsiella variicola with the deposit number CCUG 10925 (S5 for short); catellibacterium terrae collection number BMZ065747 (S6) was purchased from Ningbo Ming boat Biotechnology, inc. at 9/06/2019 under the https:// www.mingzhoubio.com /)
The present invention will be further illustrated by the following specific examples.
Example 1:
respectively culturing S1, S2, S3, S4, S5 and S6 single strains (NM 2) to a logarithmic growth phase, respectively inoculating each single strain to a sterilized heterotrophic nitrification culture medium (HNM), a eutrophic denitrification culture medium 1 (NM 1) and a eutrophic denitrification culture medium 2 (NM 2), and monitoring the removal effect of each strain on ammonia nitrogen, nitrate nitrogen and nitrite nitrogen under the condition that dissolved oxygen is 6.0-8.0mg/L, wherein the obtained result is shown in figure 1.
The heterotrophic nitrification culture medium (HNM) (g/L) (NH) 4 ) 2 SO 4 0.47, trisodium citrate 1.225, 50mL vickers salt solution, pH 7.0. Wherein Vickers' salt solution (. Mu.g/L) K 2 HPO 4 5.0，FeSO 4 ·7H 2 O 0.05，NaCl 2.5，MgSO 4 ·7H 2 O 2.5，MnSO 4 ·H 2 O 0.04。
The aerobic denitrification culture medium 1 (NM 1) (g/L) comprises trisodium citrate 1.13 and KNO 3 0.72，MgSO 4 ·7H 2 O 1.0，KH 2 PO 4 1.0，pH 7.0。
The aerobic denitrification culture medium 2 (NM 2) (g/L) comprises trisodium citrate 1.13 and NaNO 2 0.5，MgSO 4 ·7H 2 O 1.0，KH 2 PO 4 1.0，pH 7.0。
As can be seen from FIG. 1, S2, S3, S4, S5 and S6 are directed to NH in the above-mentioned medium (C/N is 3) 4 + -N removal between 32.4-44.1%; for NO 3 - -N and NO 2 - The removal rate of-N can reach more than 94.5 percent, and the denitrification performance is better.
Example 2
The heterotrophic nitrification-aerobic denitrification bacterial agent is prepared by the following method:
taking OD 600 S1, S2, S3, S4, S5, and S6 of 1 ± 0.1 volume ratio 1.
The denitrification effect of the microbial inoculum prepared by the embodiment is examined by taking natural water (actual river water of a certain secondary river) in Tianjin city.
Respectively taking S1, S2, S3, S4, S5 and S6 with equal volume from 6 containers, mixing uniformly, adding into 200ml of actual river water according to the volume ratio of 1%, wherein the ammonia Nitrogen (NH) of the river water 4 + -N), nitrate Nitrogen (NO) 3 - -N), nitrite Nitrogen (NO) 2 - -N), biochemistryOxygen Demand (COD) cr ) 1.869mg/L, 1.065mg/L, 0.079mg/L and 15mg/L respectively, and the carbon-nitrogen ratio (C/N) of the river water is about 2. Culturing in the same volume of real river water without any microbial inoculum as control group at 30 deg.C with dissolved oxygen of 6.0-8.0mg/L to obtain NH in river water 4 + -N、NO 3 - -N、NO 2 - The concentration of-N was monitored for three consecutive days, and the results are shown in FIG. 2.
As can be seen from FIG. 2, the addition of the microbial inoculum of the embodiment significantly improves the denitrification effect of river water. Microbial inoculum pair NO 3 - The highest removal rate of N can reach 100 percent, and the final removal rate is 97.46 percent; NO 2 - The removal rate of-N was 91.14%. Adding the microbial inoculum, NH in river water 4 + The N concentration was 57.57% on the first day and increased on the second day, but was always significantly lower than the NH of the control group 4 + -N concentration. The result shows that the microbial inoculum has strong denitrification effect on the river water and can be used for actual denitrification and purification of the river water.
Claims (3)
1. Heterotrophic nitrification-aerobic denitrification bacteria agent is characterized in that the agent is made of Pseudomonas stutzeri with the preservation number CCUG 12913; pseudomonas sp. deposit number DSM 25830; pseudomonas sp. deposit No. DSM 25356; bar Li Ali Pseudomonas balerarcia accession number CCUG 08492; klebsiella variicola with Klebsiella variicola deposit number CCUG 10925; and Catellibacter terrae accession number BMZ 065747.
2. The heterotrophic nitrification-aerobic denitrification bacterial agent according to claim 1, wherein said Pseudomonas stutzeri collection number CCUG 12913, pseudomonas sp collection number DSM 25830, pseudomonas sp collection number DSM 25356, P Li Ali Pseudomonas balacia collection number CCUG 08492, K.mutans Klebsiella variicola collection number CCUG 10925 and C.mutans collection number BMZ065747 are in OD 600 1 ± 0.1, volume ratio of 1.
3. The use of the heterotrophic nitrification-aerobic denitrification agent of claim 1 or 2 in natural water purification.
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| CN111039415A (en) * | 2019-11-18 | 2020-04-21 | 天津大学 | Preparation method of immobilized slow-release microorganism for in-situ restoration of water body |
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Patent Citations (4)
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| JP2015180194A (en) * | 2014-03-03 | 2015-10-15 | 国立大学法人宇都宮大学 | Microbiologic agent, microorganism flock and method for producing the same |
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| CN109402016A (en) * | 2018-09-21 | 2019-03-01 | 江苏宜裕环保科技有限公司 | For the complex micro organism fungicide of chemical wastewater treatment and its screening and preparation method |
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