CN111296487A - Method for improving saline-alkali tolerance of corn seedlings and improving physicochemical characteristics of rhizosphere soil of corn seedlings - Google Patents

Method for improving saline-alkali tolerance of corn seedlings and improving physicochemical characteristics of rhizosphere soil of corn seedlings Download PDF

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CN111296487A
CN111296487A CN202010088664.3A CN202010088664A CN111296487A CN 111296487 A CN111296487 A CN 111296487A CN 202010088664 A CN202010088664 A CN 202010088664A CN 111296487 A CN111296487 A CN 111296487A
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杨丽娟
杨克军
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Heilongjiang Bayi Agricultural University
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Abstract

The invention discloses a method for improving saline-alkali tolerance of corn seedlings and improving physicochemical characteristics of rhizosphere soil of the corn seedlings. The invention protects the application of Klebsiella variicola in any one of the following (a1) - (a 6): (a1) promoting the growth of the corn; (a2) promoting the growth of the corn in the saline-alkali environment; (a3) improving the activity of the soil enzyme of the corn rhizosphere; (a4) improving the activity of the corn rhizosphere soil enzyme in the saline-alkali environment; (a5) the saline-alkali tolerance of the corn is improved; (a6) the saline-alkali tolerance of the corn is improved by improving the activity of the corn rhizosphere soil enzyme in the saline-alkali environment. In addition, the method is high in safety, suitable for popularization and transformation and has important significance for improving root system micro-ecological environment and promoting the growth of the corn seedlings under saline-alkali stress.

Description

Method for improving saline-alkali tolerance of corn seedlings and improving physicochemical characteristics of rhizosphere soil of corn seedlings
Technical Field
The invention relates to a method for improving saline-alkali tolerance of corn seedlings and improving physicochemical characteristics of rhizosphere soil of the corn seedlings.
Background
The saline-alkali soil in China has huge area, the development of the planting industry is severely limited, and the corn which is sensitive to saline-alkali components is also deeply influenced. How to improve the microenvironment of rhizosphere soil of corn, particularly corn seedling stage, and improve the saline-alkali tolerance of corn seedlings, has great significance for the sustainable development of agriculture in saline-alkali areas. For a long time, people mostly adopt a chemical regulation and control method to improve the saline-alkali tolerance of corn seedlings, and the method has serious chemical residue, worsens the soil environment and is not beneficial to agricultural sustainable development. In recent years, with the development of molecular biology, researches on improving the saline-alkali tolerance of corn seedlings through a molecular breeding technology are gradually increased, but some molecular breeding means, such as a transgenic technology and the like, have great disputes on safety and have prospects to be investigated.
Disclosure of Invention
The invention aims to provide a method for improving saline-alkali tolerance of corn seedlings and improving physicochemical characteristics of rhizosphere soil of the corn seedlings.
In a first aspect, the present invention is the first to protect the use of Klebsiella variicola in any one of (a1) - (a6) as follows:
(a1) promoting the growth of the corn;
(a2) promoting the growth of the corn in the saline-alkali environment;
(a3) improving the activity of the soil enzyme of the corn rhizosphere;
(a4) improving the activity of the corn rhizosphere soil enzyme in the saline-alkali environment;
(a5) the saline-alkali tolerance of the corn is improved;
(a6) the saline-alkali tolerance of the corn is improved by improving the activity of the corn rhizosphere soil enzyme in the saline-alkali environment.
In a second aspect, the invention provides the use of a combination of Klebsiella variicola and Bacillus licheniformis in any one of the following (a1) - (a 6):
(a1) promoting the growth of the corn;
(a2) promoting the growth of the corn in the saline-alkali environment;
(a3) improving the activity of the soil enzyme of the corn rhizosphere;
(a4) improving the activity of the corn rhizosphere soil enzyme in the saline-alkali environment;
(a5) the saline-alkali tolerance of the corn is improved;
(a6) the saline-alkali tolerance of the corn is improved by improving the activity of the corn rhizosphere soil enzyme in the saline-alkali environment.
In the first and second aspects, the promotion of the growth of corn is to increase the plant height and/or the dry weight of the overground part and/or the dry weight of the underground part and/or the grain weight and/or the yield of the corn; the method for improving the activity of the corn rhizosphere soil enzyme in the saline-alkali environment is to improve the activity of urease and/or alkaline phosphatase and/or sucrase and/or catalase in the corn rhizosphere soil in the saline-alkali environment. The grain weight may specifically be a hundred grain weight. The yield may in particular be a yield at 14% moisture content.
In the second aspect, the bacteria content ratio of the Klebsiella variicola to the Bacillus licheniformis is 0.5-3: 1.
The bacteria content ratio of the Klebsiella variicola to the Bacillus licheniformis can be specifically 0.5:1 (1: 2 in the embodiment), 1:1, 2:1 or 3: 1. Among them, 2:1 is most preferable.
In a third aspect, the invention protects any one of the following microbial agents;
and (3) microbial inoculum A: comprises Klebsiella variicola;
and (3) bacterial agent B: a bacterial preparation containing Klebsiella variicola and Bacillus licheniformis.
The microbial inoculum A can specifically contain 1 multiplied by 102cfu/ml～1×108cfu/ml of Klebsiella variicola. More specifically, the microbial agent A may contain 1X 102cfu/ml、1×104cfu/m、1×106cfu/ml (optimal) or 1X 108cfu/ml of Klebsiella variicola.
The microbial inoculum B can specifically contain 1 multiplied by 102cfu/ml～1×108cfu/ml of Klebsiella variicola and Bacillus licheniformis; the bacteria content ratio of the Klebsiella variicola to the Bacillus licheniformis is 0.5-3: 1. More specifically, the microbial agent B may contain 1X 106cfu/ml of Klebsiella variicola and Bacillus licheniformis. The bacteria content ratio of the Klebsiella variicola to the Bacillus licheniformis can be specifically 0.5:1 (1: 2 in the embodiment), 1:1, 2:1 or 3: 1. Among them, 2:1 is most preferable.
In a fourth aspect, the present invention protects the use of the microbial agent of the third aspect in any one of the following (a1) to (a 6):
(a1) promoting the growth of the corn;
(a2) promoting the growth of the corn in the saline-alkali environment;
(a3) improving the activity of the soil enzyme of the corn rhizosphere;
(a4) improving the activity of the corn rhizosphere soil enzyme in the saline-alkali environment;
(a5) the saline-alkali tolerance of the corn is improved;
(a6) the saline-alkali tolerance of the corn is improved by improving the activity of the corn rhizosphere soil enzyme in the saline-alkali environment.
In the fourth aspect, the promotion of the growth of the corn is to increase the plant height and/or the dry weight of the overground part and/or the dry weight of the underground part and/or the grain weight and/or the yield of the corn; the method for improving the activity of the corn rhizosphere soil enzyme in the saline-alkali environment is to improve the activity of urease and/or alkaline phosphatase and/or sucrase and/or catalase in the corn rhizosphere soil in the saline-alkali environment. The grain weight may specifically be a hundred grain weight. The yield may in particular be a yield at 14% moisture content.
In a fifth aspect, the invention provides method a or method B or method C;
the method A for promoting the growth of the corns in the saline-alkali environment comprises the following steps: applying any one of (b1) to (b3) below to corn root or rhizosphere soil;
the method B for improving the activity of the corn rhizosphere soil enzyme in the saline-alkali environment comprises the following steps: applying any one of (b1) to (b3) below to corn root or rhizosphere soil;
the method C is a method for improving the saline-alkali tolerance of the corn by improving the activity of the corn rhizosphere soil enzyme in the saline-alkali environment, and comprises the following steps: applying any one of (b1) to (b3) below to corn root or rhizosphere soil;
(b1) klebsiella variicola;
(b2) klebsiella variicola and Bacillus licheniformis;
(b3) the microbial inoculum as described hereinbefore.
In the fifth aspect, the promotion of the growth of corn is to increase the plant height and/or the dry weight of the above-ground parts and/or the dry weight of the below-ground parts and/or the grain weight and/or the yield of corn; the method for improving the activity of the corn rhizosphere soil enzyme in the saline-alkali environment is to improve the activity of urease and/or alkaline phosphatase and/or sucrase and/or catalase in the corn rhizosphere soil in the saline-alkali environment. The grain weight may specifically be a hundred grain weight. The yield may in particular be a yield at 14% moisture content. When the Klebsiella variicola and the Bacillus licheniformis are used together, the bacteria content ratio of the Klebsiella variicola to the Bacillus licheniformis can be specifically 0.5:1 (1: 2 in the embodiment), 1:1, 2:1 or 3: 1. Among them, 2:1 is most preferable.
In the method, when the corn is cultivated in a pot, any one of (b1) - (b3) can be applied to the soil after sowing, and the application dosage can be 60ml of bacterial suspension or microbial inoculum applied to every 300g of soil; the concentration of the bacteria in the bacterial suspension or the bacterial agent is 1 multiplied by 102cfu/ml～1×108cfu/ml (preferably 1X 10)6cfu/ml)。
In the above method, when corn is cultivated in a field mode, any one of (b1) - (b3) can be specifically applied to the soil after sowing, and the application dosage can be specifically 150ml of bacterial suspension or microbial inoculum; the concentration of the bacteria in the bacterial suspension or the bacterial agent is 1 multiplied by 102cfu/ml～1×108cfu/ml (preferably 1X 10)6cfu/ml)。
Any one of the Klebsiella variicola specifically can be Klebsiella variicola with the serial number of CGMCC 1.15640 from China general microbiological culture Collection management center.
Any one of the bacillus licheniformis can be bacillus licheniformis which is from China general microbiological culture Collection center and has the number of CGMCC 1.7677.
Any one of the saline-alkali environments can be specifically natural saline-alkali soil, and the natural saline-alkali soil can be specifically pH9.2, Na+The content of the extract is 0.906 g/kg-1The natural saline-alkali soil.
Any of the above-described maize specifically may be corn 335.
The invention has the following advantages: (1) the saline-alkali tolerance of the corn seedlings under saline-alkali stress is remarkably improved, and the dry weight of the overground part, the dry weight of the underground part and the plant height are remarkably increased. The urease activity, the alkaline phosphatase activity, the sucrase activity and the catalase activity of the rhizosphere soil of the corn seedlings are all obviously improved. (2) The viable bacteria agent is adopted, chemical components such as chemical fertilizers and pesticides are not contained, and environmental pollution is avoided, so that the development requirement of green agriculture is met. (3) The strain can be colonized in soil for a long time, has strong persistence and has beneficial effect on corn planting for years in the future.
In addition, the method is high in safety, suitable for popularization and transformation and has important significance for improving root system micro-ecological environment and promoting the growth of the corn seedlings under saline-alkali stress.
Drawings
FIG. 1 is a graph showing the statistics of urease activity, alkaline phosphatase activity, sucrase activity and catalase activity for various groups of corn in example 1.
FIG. 2 is the results of corn phenotype observations of control and experimental groups in example 1.
FIG. 3 is a graph showing the statistics of urease activity, alkaline phosphatase activity, sucrase activity and catalase activity for various groups of corn in example 2.
Detailed Description
The following examples are given to facilitate a better understanding of the invention, but do not limit the invention. The experimental procedures in the following examples are conventional unless otherwise specified. The test materials used in the following examples were purchased from a conventional biochemical reagent store unless otherwise specified. The quantitative tests in the following examples, all set up three replicates and the results averaged.
Klebsiella variicola: china general microbiological culture Collection center (CGMCC 1.15640).
B, bacillus licheniformis: china general microbiological culture Collection center (CGMCC 1.7677).
Firstly, jade 335 corn: purchased from Heilongjiang Fuzun agriculture Integrated services linkage Limited.
LB liquid medium: 10g of peptone, 5g of yeast extract powder, 10g of sodium chloride, 1L of distilled water and pH 7.0.
Example 1 Klebsiella variicola used for improving saline-alkali tolerance of corn seedlings and improving physicochemical characteristics of rhizosphere soil of corn seedlings
Firstly, preparation of bacterial suspension
Inoculating Klebsiella variicola into LB liquid culture medium, culturing at 30 deg.C and 180rpm to obtain bacterial suspension containing enough bacteria, and culturing the bacterial suspension at 6000 r.min-1Centrifuging for 15min, discarding supernatant, washing precipitate with sterile distilled water, resuspending, repeating for 3 times, and concocting to 1 × 108cfu/ml bacterial suspension, and then respectively diluted to 1 × 10 according to experimental needs2cfu/ml、1×104cfu/ml、1×106cfu/ml for standby.
Secondly, accelerating germination of corn
Screening corn seeds 335, selecting corn seeds with same size and undamaged surface, soaking and sterilizing with sodium hypochlorite (concentration 10%) for 10min, and washing with sterile water for more than 5 times until there is no obvious sodium hypochlorite taste. And soaking the sterilized seeds in sterile water, placing the seeds in a constant-temperature incubator, and swelling for 6 hours, and discarding the sterile water used for swelling after swelling for 6 hours. And (3) laying a piece of filter paper with the same area as the bottom of the box in the germination box, and uniformly dropwise adding sterile water, wherein the whole piece of filter paper can be soaked by the water and slightly overflows. The seeds after imbibition are evenly placed in a germination box, 20 seeds are placed in each box, a piece of filter paper is laid on the seeds, 3ml of sterile water is evenly dripped into each piece of paper, and the germination box is covered and covered in a cover and is placed in an incubator at 25 ℃ in the dark for accelerating germination for 24 hours.
Third, preparation of potted plant
The potting soil was thoroughly air-dried, sieved through a 2mm sieve, and 200g of soil was placed in each pot (specification: length. times. width. times. height: 10 cm. times. 12 cm). The pot culture soil is natural saline-alkali soil (pH9.2, Na)+The content of the extract is 0.906 g/kg-1) Is taken from the Daqing city of Heilongjiang province. And (3) selecting corn seeds with the bud length of about 1cm (0.9cm-1.1cm) after accelerating germination for 24 hours in the step two, planting the corn seeds into pot pots filled with soil, uniformly sowing 5 seeds in each pot, and covering 100g of soil above each pot of seeds.
Fourthly, inoculating bacterial suspension
And (3) pouring 60ml of the bacterial suspension with different concentrations obtained in the step one into the pot covered with the soil in the step three (when 60ml of the bacterial suspension is added into every 300g of soil, the water content of the soil is appropriate and is consistent with the water content of the conventional corn cultivation). Placing the pot in a light incubator for culturing for 9 days; 10ml of sterile water was added to each pot at 48 hour intervals during the cultivation. A control group was set without added bacterial suspension.
The culture conditions were: the day and night temperature is 25 ℃/20 ℃, the illumination is 12h every day, and the humidity is 60-80%.
And after 9 days of culture, counting the plant height, the dry weight of the overground part and the dry weight of the underground part of the corn seedling, and counting the rhizosphere soil urease activity, the alkaline phosphatase activity, the sucrase activity and the catalase activity of the corn seedling.
The plant height measuring method comprises the following steps: and measuring in a pot, respectively selecting corn seedlings with the same growth vigor in each treatment, straightening the leaves, and measuring the length from the soil surface to the top end of the highest leaf by using a ruler.
Method for measuring the dry weight of the above-ground parts: respectively taking corn seedlings with the same growth vigor in each treatment, cutting the corn seedlings at the root-stem junction, putting stem and leaf parts into a drying oven, drying to constant weight at 80 ℃, and weighing the dry weight of the overground part.
Method for measuring dry weight of underground part: respectively taking corn seedlings with the same growth vigor in each treatment, cutting the corn seedlings at the root-stem junction, putting roots into a drying oven, drying to constant weight at 80 ℃, and weighing the dry weight of underground parts.
Methods for detecting urease activity, alkaline phosphatase activity, sucrase activity and catalase activity are described in the following references: guanguanyin, soil enzyme and its research method [ M ]. agricultural Press, 1986.
The results are shown in fig. 1, fig. 2 and table 1. The results showed that the height of maize seedlings, the dry weight of the above-ground parts and the dry weight of the below-ground parts all increased significantly after 9 days of culture (P)<0.05) when the concentration of the bacteria reaches 106When cfu/ml, each index basically reaches (approaches) the maximum value, the bacterial concentration is continuously increased, and when the bacterial concentration is 108Each index and the bacterial concentration are 10 when cfu/ml6The cfu/ml ratio is not increased significantly, so that the economic cost and the effect are combined, and the like, and finally the final determination is 106cfu/ml is the optimum addition amount. Is less thanCompared with a control group (CK) added with the bacterial suspension, the plant height is increased by 38.11%, the dry weight of the overground part is increased by 79.76%, and the dry weight of the underground part is increased by 72.22%. The activity of various enzymes in the rhizosphere soil of the corn seedlings is obviously improved (P)<0.05), 64.22% of urease activity, 61.90% of alkaline phosphatase activity, 50.26% of sucrase activity and 49.21% of catalase activity.
TABLE 1
Figure BDA0002382954050000051
Figure BDA0002382954050000061
The results show that the Klebsiella variicola can obviously improve the root system micro-ecological environment and promote the growth of corn seedlings under saline-alkali stress.
Example 2 combination of Klebsiella variicola and Bacillus licheniformis for improving saline-alkali tolerance of corn seedlings and improving physicochemical properties of rhizosphere soil of corn seedlings
Firstly, preparation of bacterial suspension
Inoculating Klebsiella variicola into LB liquid culture medium, culturing at 30 deg.C and 180rpm to obtain bacterial suspension containing enough bacteria, and culturing the bacterial suspension at 6000 r.min-1Centrifuging for 15min, discarding supernatant, washing precipitate with sterile distilled water, resuspending, repeating for 3 times, and concocting to 1 × 106cfu/ml bacterial suspension.
Inoculating Bacillus licheniformis into LB liquid culture medium, culturing at 30 deg.C and 180rpm to obtain bacterial suspension containing sufficient bacteria, and culturing the bacterial suspension at 6000 r.min-1Centrifuging for 15min, discarding supernatant, washing precipitate with sterile distilled water, resuspending, repeating for 3 times, and concocting to 1 × 106cfu/ml bacterial suspension.
Mixing Klebsiella variicola suspension (concentration 1 × 10)6cfu/ml) and Bacillus licheniformis suspension (concentration 1X 10)6cfu/ml) were mixed at different volume ratios to give the following bacterial suspensions (total volume of each bacterial suspension was 60 ml):
bacterial suspension (1: 0): the concentration is 1X 106cfu/ml of Klebsiella variicola suspension. 60ml of bacterial suspension contains about 6X 107cfu of Klebsiella variicola.
Bacterial suspension (3: 1): 3 parts by volume of 1X 106cfu/ml Klebsiella variicola suspension and 1 volume part concentration of 1X 106cfu/ml of Bacillus licheniformis suspension. 60ml of bacterial suspension together contain about 4.5X 107Klebsiella variicola cfu and about 1.5X 107cfu Bacillus licheniformis.
Bacterial suspension (2: 1): 2 parts by volume of 1X 106cfu/ml Klebsiella variicola suspension and 1 volume part concentration of 1X 106cfu/ml of Bacillus licheniformis suspension. 60ml of bacterial suspension together contains about 4X 107Klebsiella variicola cfu and about 2X 107cfu Bacillus licheniformis.
Bacterial suspension (1: 1): 1 part by volume of 1X 106cfu/ml Klebsiella variicola suspension and 1 volume part concentration of 1X 106cfu/ml of Bacillus licheniformis suspension. 60ml of bacterial suspension together contains about 3X 107Klebsiella variicola cfu and about 3X 107cfu Bacillus licheniformis.
Bacterial suspension (1: 2): 1 part by volume of 1X 106cfu/ml Klebsiella variicola suspension and 2 parts by volume concentration of 1X 106cfu/ml of Bacillus licheniformis suspension. 60ml of bacterial suspension contains about 2X 107Klebsiella variicola cfu and about 4X 107cfu Bacillus licheniformis.
Bacterial suspension (0: 1): the concentration is 1X 106cfu/ml of Bacillus licheniformis suspension. 60ml of bacterial suspension contains about 6X 107cfu Bacillus licheniformis.
Secondly, accelerating germination of corn
Screening corn seeds 335, selecting corn seeds with same size and undamaged surface, soaking and sterilizing with sodium hypochlorite (concentration 10%) for 10min, and washing with sterile water for more than 5 times until there is no obvious sodium hypochlorite taste. And soaking the sterilized seeds in sterile water, placing the seeds in a constant-temperature incubator, and swelling for 6 hours, and discarding the sterile water used for swelling after swelling for 6 hours. And (3) laying a piece of filter paper with the same area as the bottom of the box in the germination box, and uniformly dropwise adding sterile water, wherein the whole piece of filter paper can be soaked by the water and slightly overflows. The seeds after imbibition are evenly placed in a germination box, 20 seeds are placed in each box, a piece of filter paper is laid on the seeds, 3ml of sterile water is evenly dripped into each piece of paper, and the germination box is covered and covered in a cover and is placed in an incubator at 25 ℃ in the dark for accelerating germination for 24 hours.
Third, preparation of potted plant
The potting soil was thoroughly air-dried, sieved through a 2mm sieve, and 200g of soil was placed in each pot (specification: length. times. width. times. height: 10 cm. times. 12 cm). The pot culture soil is natural saline-alkali soil (pH9.2, Na)+The content of the extract is 0.906 g/kg-1) Is taken from the Daqing city of Heilongjiang province. And (3) selecting corn seeds with the bud length of about 1cm (0.9cm-1.1cm) after accelerating germination for 24 hours in the step two, planting the corn seeds into pot pots filled with soil, uniformly sowing 5 seeds in each pot, and covering 100g of soil above each pot of seeds.
Fourthly, inoculating bacterial suspension
And (3) pouring 60ml of the bacterial suspension with different concentrations obtained in the step one into the pot covered with the soil in the step three (when 60ml of the bacterial suspension is added into every 300g of soil, the water content of the soil is appropriate and is consistent with the water content of the conventional corn cultivation). Placing the pot in a light incubator for culturing for 9 days; 10ml of sterile water was added to each pot at 48 hour intervals during the cultivation.
The culture conditions were: the day and night temperature is 25 ℃/20 ℃, the illumination is 12h every day, and the humidity is 60-80%.
And after 9 days of culture, counting the plant height, the dry weight of the overground part and the dry weight of the underground part of the corn seedling, and counting the rhizosphere soil urease activity, the alkaline phosphatase activity, the sucrase activity and the catalase activity of the corn seedling.
The results are shown in table 2 and fig. 3. The result shows that the addition ratio of the Klebsiella variicola to the Bacillus licheniformis is optimized to obtain the ratio of the Klebsiella variicola: bacillus licheniformis 2: the best 1 time, the plant height reaches 33.267cm, the dry weight of the overground part reaches 0.160g, and the dry weight of the underground part reaches 0.101 g. The activity of various enzymes of the rhizosphere soil of the corn seedlings is obviously improved (P is less than 0.05), the activity of urease reaches 0.717mg/g, the activity of alkaline phosphatase reaches 0.832mg/g, the activity of sucrase reaches 31.077mg/g, and the activity of catalase reaches 2.794 mg/g.
TABLE 2
Figure BDA0002382954050000071
Bacteria: bacillus licheniformis)
Figure BDA0002382954050000081
Example 3 field experiment
First, experiment site
The test practice base of the university of eight agricultural reclamation of Heilongjiang, the test soil is saline-alkali soil (pH8.9, Na)+The content of the extract is 0.891 g/kg-1)。
Second, experiment time
A total of two experiments were performed.
The first batch of seeding time: year 2018, 5 month 25 day, harvest time: year 2018, month 10 and day 6.
And (3) sowing time of the second batch: year 2019, month 5, day 20, harvest time: year 2019, month 10 and day 3.
Third, Experimental methods
1. Before sowing, the land mass is uniformly subjected to conventional continuous field operations such as rotary tillage stubble cleaning, ploughing and leveling after ploughing, fertilizing, ridging, pressing and the like.
2. The operation is performed in the following groups:
control group (CK): corn seeds 335 are sown, 150ml of water is added into the soil during sowing, and 200g of soil is covered above the sowing position.
Experimental group (T1): sowing corn seeds 335, adding 150ml of 1 × 10 concentration into soil during sowing6cfu/ml Klebsiella variicola suspension (bacterial suspension preparation method as shown in example 2) was covered with 200g of soil over the sown site.
Experimental group (T2): sowing corn seeds 335, adding 100ml of corn seeds with concentration of 1 × 106Habitat of cfu/mlKlebsiella suspension (preparation of the suspension is described in example 2) and 50ml of 1X 106cfu/ml of Bacillus licheniformis suspension (preparation of suspension is as shown in example 2) and 200g of soil is placed on the upper part of the sowing area.
The addition amount of water or bacterial suspension in each group is determined through preliminary experiments, when the addition amount is 150ml, the bacterial liquid can reach the position 30cm below the ground surface, and the reaching range of the bacterial liquid is consistent with the root growth range of the corn seedling stage.
Each of the above treatments was set to 6 lines each 20m long and 0.6m wide, and each treatment was repeated 3 times.
The field management methods of the treatment groups are consistent, and the nitrogen fertilizer is applied to the field in the jointing stage and the large-horn mouth stage according to the conventional method of 70kg/hm2Thinning is carried out according to a conventional method in the two-leaf period of the corn, and irrigation is carried out according to rainfall and the water content of the soil in the field.
3. 3 of the seeds are harvested at 30m per treatment2And (4) sampling, checking the number of effective spikes in the sampling, measuring the weight and the water content of fresh grains after artificial threshing, and calculating the yield under the water content of 14%. 10 ears of each treatment group were taken for seed test, and the ear row number, the row grain number and the hundred grain weight were measured.
The results are shown in Table 3. The result shows that the Klebsiella variicola is used for corn cultivation, and the corn yield on the saline-alkali soil can be improved.
TABLE 3
Figure BDA0002382954050000091

Claims (10)

1. Use of Klebsiella variicola in any one of (a1) - (a6) as follows:
(a1) promoting the growth of the corn;
(a2) promoting the growth of the corn in the saline-alkali environment;
(a3) improving the activity of the soil enzyme of the corn rhizosphere;
(a4) improving the activity of the corn rhizosphere soil enzyme in the saline-alkali environment;
(a5) the saline-alkali tolerance of the corn is improved;
(a6) the saline-alkali tolerance of the corn is improved by improving the activity of the corn rhizosphere soil enzyme in the saline-alkali environment.
2. The application of the combination of the Klebsiella variicola and the Bacillus licheniformis in any one of the following (a1) - (a 6):
(a1) promoting the growth of the corn;
(a2) promoting the growth of the corn in the saline-alkali environment;
(a3) improving the activity of the soil enzyme of the corn rhizosphere;
(a4) improving the activity of the corn rhizosphere soil enzyme in the saline-alkali environment;
(a5) the saline-alkali tolerance of the corn is improved;
(a6) the saline-alkali tolerance of the corn is improved by improving the activity of the corn rhizosphere soil enzyme in the saline-alkali environment.
3. Use according to claim 1 or 2, characterized in that:
the promotion of the growth of the corn is to improve the plant height and/or the dry weight of the overground part and/or the dry weight of the underground part and/or the grain weight and/or the yield of the corn; the method for improving the activity of the corn rhizosphere soil enzyme in the saline-alkali environment is to improve the activity of urease and/or alkaline phosphatase and/or sucrase and/or catalase in the corn rhizosphere soil in the saline-alkali environment.
4. Use according to claim 2 or 3, characterized in that: the bacteria content ratio of the Klebsiella variicola to the Bacillus licheniformis is 0.5-3: 1.
5. Any one of the following microbial agents;
and (3) microbial inoculum A: comprises Klebsiella variicola;
and (3) bacterial agent B: a bacterial preparation containing Klebsiella variicola and Bacillus licheniformis.
6. The microbial inoculum of claim 5, wherein:
the microbial inoculum A contains 1 multiplied by 102cfu/ml～1×108cfu/ml of Klebsiella variicola;
the microbial inoculum B contains 1 multiplied by 102cfu/ml～1×108cfu/ml of Klebsiella variicola and Bacillus licheniformis; the bacteria content ratio of the Klebsiella variicola to the Bacillus licheniformis is 0.5-3: 1.
7. The use of the bacterial agent of claim 5 or 6 in any one of the following (a1) - (a 6):
(a1) promoting the growth of the corn;
(a2) promoting the growth of the corn in the saline-alkali environment;
(a3) improving the activity of the soil enzyme of the corn rhizosphere;
(a4) improving the activity of the corn rhizosphere soil enzyme in the saline-alkali environment;
(a5) the saline-alkali tolerance of the corn is improved;
(a6) the saline-alkali tolerance of the corn is improved by improving the activity of the corn rhizosphere soil enzyme in the saline-alkali environment.
8. The use of claim 7, wherein:
the promotion of the growth of the corn is to improve the plant height and/or the dry weight of the overground part and/or the dry weight of the underground part and/or the grain weight and/or the yield of the corn; the method for improving the activity of the corn rhizosphere soil enzyme in the saline-alkali environment is to improve the activity of urease and/or alkaline phosphatase and/or sucrase and/or catalase in the corn rhizosphere soil in the saline-alkali environment.
9. Method A or method B or method C;
the method A for promoting the growth of the corns in the saline-alkali environment comprises the following steps: applying any one of (b1) to (b3) below to corn root or rhizosphere soil;
the method B for improving the activity of the corn rhizosphere soil enzyme in the saline-alkali environment comprises the following steps: applying any one of (b1) to (b3) below to corn root or rhizosphere soil;
the method C is a method for improving the saline-alkali tolerance of the corn by improving the activity of the corn rhizosphere soil enzyme in the saline-alkali environment, and comprises the following steps: applying any one of (b1) to (b3) below to corn root or rhizosphere soil;
(b1) klebsiella variicola;
(b2) klebsiella variicola and Bacillus licheniformis;
(b3) the microbial preparation according to claim 5 or 6.
10. The method of claim 9, wherein:
the promotion of the growth of the corn is to improve the plant height and/or the dry weight of the overground part and/or the dry weight of the underground part and/or the grain weight and/or the yield of the corn; the method for improving the activity of the corn rhizosphere soil enzyme in the saline-alkali environment is to improve the activity of urease and/or alkaline phosphatase and/or sucrase and/or catalase in the corn rhizosphere soil in the saline-alkali environment.
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