CN116590170B - Preparation and application of microorganism strain, compound strain and growth-promoting disease-preventing organic fertilizer - Google Patents
Preparation and application of microorganism strain, compound strain and growth-promoting disease-preventing organic fertilizer Download PDFInfo
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- 238000002360 preparation method Methods 0.000 title claims abstract description 16
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- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title abstract description 17
- 244000005700 microbiome Species 0.000 title abstract description 12
- 238000000855 fermentation Methods 0.000 claims abstract description 54
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- 238000004321 preservation Methods 0.000 claims description 20
- 238000000034 method Methods 0.000 claims description 17
- 230000001580 bacterial effect Effects 0.000 claims description 15
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 11
- 239000002131 composite material Substances 0.000 claims description 11
- 241000223259 Trichoderma Species 0.000 claims description 10
- 239000002068 microbial inoculum Substances 0.000 claims description 3
- 239000003337 fertilizer Substances 0.000 abstract description 63
- 230000000694 effects Effects 0.000 abstract description 39
- 230000001737 promoting effect Effects 0.000 abstract description 18
- 239000000126 substance Substances 0.000 abstract description 10
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- 238000011160 research Methods 0.000 abstract description 3
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- 241000223218 Fusarium Species 0.000 description 10
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 3
- 230000000844 anti-bacterial effect Effects 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 239000008367 deionised water Substances 0.000 description 3
- 229910021641 deionized water Inorganic materials 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
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- 238000009335 monocropping Methods 0.000 description 3
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- 229910052698 phosphorus Inorganic materials 0.000 description 3
- 229910052700 potassium Inorganic materials 0.000 description 3
- 238000004382 potting Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
- 244000269722 Thea sinensis Species 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
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- 239000008272 agar Substances 0.000 description 2
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- 239000004202 carbamide Substances 0.000 description 2
- 238000013329 compounding Methods 0.000 description 2
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- 239000012895 dilution Substances 0.000 description 2
- YWEUIGNSBFLMFL-UHFFFAOYSA-N diphosphonate Chemical compound O=P(=O)OP(=O)=O YWEUIGNSBFLMFL-UHFFFAOYSA-N 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
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- 244000052769 pathogen Species 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- DLYUQMMRRRQYAE-UHFFFAOYSA-N phosphorus pentoxide Inorganic materials O1P(O2)(=O)OP3(=O)OP1(=O)OP2(=O)O3 DLYUQMMRRRQYAE-UHFFFAOYSA-N 0.000 description 2
- 244000000003 plant pathogen Species 0.000 description 2
- 239000011591 potassium Substances 0.000 description 2
- CHWRSCGUEQEHOH-UHFFFAOYSA-N potassium oxide Chemical compound [O-2].[K+].[K+] CHWRSCGUEQEHOH-UHFFFAOYSA-N 0.000 description 2
- 229910001950 potassium oxide Inorganic materials 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 239000004576 sand Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 230000001502 supplementing effect Effects 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- 241000220485 Fabaceae Species 0.000 description 1
- 241000508192 Fusarium oxysporum f. sp. niveum Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 241000405217 Viola <butterfly> Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
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- 238000007865 diluting Methods 0.000 description 1
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- 239000004459 forage Substances 0.000 description 1
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- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000009342 intercropping Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 235000021374 legumes Nutrition 0.000 description 1
- 231100000518 lethal Toxicity 0.000 description 1
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- 239000012528 membrane Substances 0.000 description 1
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- 239000005416 organic matter Substances 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 1
- 229910052939 potassium sulfate Inorganic materials 0.000 description 1
- 235000011151 potassium sulphates Nutrition 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
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- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/885—Trichoderma
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Virology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Mycology (AREA)
- Soil Sciences (AREA)
- Botany (AREA)
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- Chemical Kinetics & Catalysis (AREA)
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Abstract
The invention discloses a microorganism strain, a compound strain and preparation and application of a growth-promoting and disease-preventing organic fertilizer, and relates to the technical field of organic fertilizers, wherein the fermentation research of vetch is carried out by screening specific disease-resistant and growth-promoting microorganisms, so that positive effects are generated on the high-valued utilization of the vetch; the organic fertilizer prepared by the invention has good effect of promoting growth and resisting diseases on crops, remarkably improves the yield and quality of the crops, can be partially used for replacing chemical fertilizers to be popularized and applied to the planting of various crops, and gradually eliminates the adverse effect of excessive application of the chemical fertilizers.
Description
Technical field:
the invention relates to the technical field of organic fertilizers, in particular to a microorganism strain, a compound strain and preparation and application of a growth-promoting disease-preventing organic fertilizer.
The background technology is as follows:
soil-borne diseases of crops are major barriers to the production of crops, and the application of control measures such as chemical pesticides and the like generally has poor control effect, pollutes the environment and induces plant pathogens to generate drug resistance. In addition, the continuous cropping obstacle of crops caused by the continuous cropping obstacle is increasingly more and more serious, and the continuous cropping obstacle becomes a serious problem for restricting the quality improvement and the synergy of agriculture in China. In order to meet the great strategic demands of the agricultural production, firstly, screening biocontrol strains aiming at crop soil-borne disease pathogenic bacteria, constructing composite strains, preparing plant materials by fermenting the constructed composite strains to prepare disease-preventing and growth-promoting organic fertilizer, supplementing beneficial microorganisms and organic matters for soil, further achieving the aims of disease prevention and growth promotion and crop quality improvement, and finally promoting the sustainable development of agriculture in China.
Sweet potatoThe genus Vicia (Fabaceae) is a herb plant. About 200 plants of the genus Viola worldwide, which are annual, perennial or perennial, and about 40 of which China includes original and introduced species, are widely used for green manure, forage grass or cover crops. At present, the species of vetch used as green manure cultivation mainly include Mao She seeds (Vicia villosa), vetch (Vicia villosa var.) and vetch (Vicia craca). The cultivation and management of the vetch are simple, the natural nitrogen fixation is realized, no fertilizer is needed, and the highest fresh grass yield can reach 45t/hm 2 The method can utilize modes of intercropping among winter idle fields, orchard tea gardens, other crops and the like to begin sowing in September to October in the current year, harvest can be carried out in the next early spring around April, other crops can be continuously planted in the harvested fields, the agricultural time is not mistaken, the land competition with grain crops is avoided, and the benefit is considerable.
In the sixty-seventies of the twentieth century, vetch is widely used as green manure in China, plays an important role in soil improvement, fertilizer culture and the like, and adopts a main method of turning over and pressing to directly return to the field. However, with the popularization and use of fertilizers in eighties, the planting area of the vetch is greatly reduced. With development of ' zero increase action of chemical fertilizer and pesticide usage ' by organizations in rural areas, the planting area of green manure crops including vetch starts to be enlarged year by year, and the green manure crops are especially favored by practitioners such as ' green agriculture ', ' organic agriculture ', ' and ' high-added-value agriculture '. The sweet potato contains abundant mineral nutrient elements, and contains pure N2.61% and P in the early flowering stage based on hay 2 O 5 0.41%, K-containing 2 O2.89%, and the stalk can be converted into organic matter improved soil for fertility improvement after being decomposed. Therefore, the green manure can replace part of fertilizers as an organic fertilizer to play important roles of promoting the yield increase of crops, improving the utilization rate of fertilizers, improving the quality of agricultural products and the like. At present, green manure crop vetch is mainly used for pressing green, and research reports on the green manure crop vetch serving as a fermentation carrier are not seen. The invention aims at taking green manure crop vetch as a fermentation carrier, adopting the screened microorganism combination to ferment the vetch to prepare the organic fertilizer for promoting growth and preventing diseases, thereby forcefully promoting the development and utilization of Mao She seed and reducing the application and enhancing the efficiency of chemical fertilizer and pesticide.
The invention comprises the following steps:
aiming at the current situations that soil-borne diseases such as corn stem rot, watermelon fusarium wilt and the like have large harm, high treatment difficulty and weight loss, and the traditional measures such as chemical pesticide application are poor in effect and environmental pollution are adopted to induce plant pathogens to generate drug resistance, the invention carries out biocontrol bacteria screening on crop soil-borne disease pathogens so as to construct microorganism bacteria combination, and the constructed composite strain is adopted to ferment a specially selected green manure carrier-vetch, so that the organic fertilizer is finally prepared, and the organic fertilizer can realize very good disease resistance and effect promotion and can also achieve the dual purposes of supplementing beneficial microorganisms and organic matters.
The technical problems to be solved by the invention are realized by adopting the following technical scheme:
the first object of the present invention is to provide a bacillus amyloliquefaciens with a preservation name of Bacillus amyloliquefaciens dhm, which is preserved in China center for type culture Collection (university of Wuhan), with a preservation date of 2022, 10 months and 18 days, and a preservation number of CCTCC NO: m20221589.
The second object of the present invention is to provide bacillus belicus with a preservation name of Bacillus velezensis dhm, which is preserved in China center for type culture Collection (university of Wuhan), with a preservation date of 2022, 10 months and 18 days, and a preservation number of CCTCC NO: m20221590.
The third object of the present invention is to provide a bacillus subtilis with a preservation name of Bacillus subtilis dhm, which is preserved in China center for type culture Collection (university of Wuhan), with a preservation date of 2022, 10 months and 18 days, and a preservation number of CCTCC NO: m20221591.
The fourth object of the present invention is to provide trichoderma harzianum with the preservation name of Trichoderma harzianum dhm, which is preserved in China center for type culture Collection (university of Wuhan), with the preservation date of 2022, 10 months and 18 days, and with the preservation number of CCTCC NO: m20221588.
The fifth object of the present invention is to provide trichoderma asperellum with a preservation name of Trichoderma asperellum dhm, which is preserved in China center for type culture Collection (university of Wuhan), with a preservation date of 2022, 10 months and 18 days, and a preservation number of cctccc NO: m20221587.
The sixth object of the invention is to provide a composite strain comprising at least two of the aforementioned bacillus amyloliquefaciens, bacillus belicus, bacillus subtilis, trichoderma harzianum and trichoderma asperellum.
The seventh object of the present invention is to provide a microbial inoculum comprising at least one of the aforementioned bacillus amyloliquefaciens, bacillus belicus, bacillus subtilis, trichoderma harzianum and trichoderma asperellum. Adjuvants can be added to make into viable bacteria preparation.
The eighth object of the invention is to provide the application of at least one of the bacillus amyloliquefaciens, bacillus belicus, bacillus subtilis, trichoderma harzianum and trichoderma asperellum in preparing organic fertilizer. Green manure crops known in the art may be used as fermentation carriers.
The ninth object of the invention is to provide an organic fertilizer, which adopts a single microorganism strain or a compound microorganism strain known in the art as a fermentation strain by taking vetch as a fermentation carrier.
The tenth object of the invention is to provide an organic fertilizer, which adopts the vetch as a fermentation carrier and adopts at least one of the bacillus amyloliquefaciens, bacillus belicus, bacillus subtilis, trichoderma harzianum and trichoderma asperellum as a fermentation bacterium.
An eleventh object of the present invention is to provide a method for preparing an organic fertilizer, wherein at least one of the aforementioned bacillus amyloliquefaciens, bacillus bailii, bacillus subtilis, trichoderma harzianum and trichoderma asperellum is inoculated onto a fermentation carrier by a primary or secondary fermentation method.
The primary fermentation method in the invention means that only primary fermentation is carried out on a fermentation carrier; the secondary fermentation method is to perform secondary fermentation on a fermentation carrier, wherein fermentation bacteria can be added during primary fermentation, or partial fermentation bacteria can be added during primary fermentation, and then other fermentation bacteria can be added during secondary fermentation, so that the fermentation effect is improved.
A twelfth object of the present invention is to provide the use of the aforementioned organic fertilizer for crop planting. The crops include various grains, vegetables, fruits known in the art.
The beneficial effects of the invention are as follows: the invention develops fermentation research of the vetch by screening specific disease-resistant growth-promoting microorganisms, and has positive effects on high-valued utilization of the vetch; the organic fertilizer prepared by the invention has good effect of promoting growth and resisting diseases on crops, remarkably improves the yield and quality of the crops, can be partially used for replacing chemical fertilizers to be popularized and applied to the planting of various crops, and gradually eliminates the adverse effect of excessive application of the chemical fertilizers.
Description of the drawings:
FIG. 1 is a phylogenetic tree of Bacillus amyloliquefaciens;
FIG. 2 is a phylogenetic tree of Bacillus beleiensis;
FIG. 3 is a phylogenetic tree of Bacillus subtilis;
FIG. 4 is a phylogenetic tree of Trichoderma harzianum;
FIG. 5 is a phylogenetic tree of Trichoderma asperellum.
The specific embodiment is as follows:
the invention is further described below with reference to specific embodiments and illustrations in order to make the technical means, the creation features, the achievement of the purpose and the effect of the implementation of the invention easy to understand.
Bacterial strain source:
bacillus amyloliquefaciens (hereinafter abbreviated as dhm 1), bacillus belicus (hereinafter abbreviated as dhm 2) and Bacillus subtilis (hereinafter abbreviated as dhm 3) are separated and purified from soil of corn planting base in Fengyang county, anhui province, sharpness, technology institute of Radar mountain corn.
Trichoderma harzianum (hereinafter abbreviated as dhm 4) and Trichoderma asperellum (hereinafter abbreviated as dhm 5) are separated and purified from planting technical garden soil in the university of Anhui science and technology university of Fengyang county in Anhui province.
Test medium:
(1) Streaking medium
5.0g of peptone, 3.0g of beef extract, 1.0g of yeast extract, 10.0g of glucose, 15.0g of agar and 1L of deionized water.
(2) Liquid fermentation medium
5.0g of peptone, 3.0g of beef extract, 1.0g of yeast extract, 10.0g of glucose and 1L of deionized water.
(3) Trichoderma culture medium
200.0g of potato (peeled), 18.0g of glucose, 15.0g of agar and 1L of deionized water.
Preparation of fermentation liquor:
diluting and streaking the dhm1, the dhm2 and the dhm3 by a streaking culture medium, culturing for 48 hours in a culture box at the temperature of 27 ℃, then picking single bacterial colony, inoculating the single bacterial colony into a liquid fermentation culture medium for culturing for 15 hours, and regulating the OD of each bacterial strain by a spectrophotometer 600 The culture broth was obtained by inoculating 0.2% (100 μl of single strain, 50 μl of each of two strains, and 33.3 μl of each of three strains) of the strain to 50mL of liquid fermentation medium, and fermenting for 72 hours.
Measurement of the effect of promotion:
the sand culture method is adopted to cultivate corn seedlings. Filling small bowls with the height of 8cm and the caliber of 5cm with sand sterilized by washing with clean water, sowing 4 corn grains in each bowl, wherein the corn sowing time is the same as the preparation starting time of strain seed liquid, and culturing in a constant temperature and humidity incubator at the temperature of 27 ℃ after sowing. And (3) after sowing for 4d, the prepared fermentation bacteria liquid is subjected to root irrigation according to the concentration (volume concentration) of 1%, 2%, 4%, 6%, 8%, 12%, 16% and 24% of bacteria liquid, the total root irrigation volume is 20mL, each concentration is repeated 6 times, the influence of the concentration of different root irrigation bacteria liquid on the whole fresh weight of corn seedlings is measured after 7d of cultivation, and the optimal root irrigation concentration of the fermentation bacteria liquid is determined.
TABLE 1 influence of different root-filling liquor concentrations and Strain combinations on the fresh weight of the whole maize seedlings
TABLE 2 influence of different root-filling liquor concentrations and Strain combinations on the fresh weight of the whole maize seedlings
As can be seen from tables 1 and 2, the fresh weight of the corn seedling is firstly increased and then decreased along with the increase of the concentration of the root-filling bacterial liquid. The concentration of the root-irrigation bacteria solution is 8% (20 mL of the total volume of the root-irrigation dilution solution, and the concentration of 8% of the fermentation bacteria solution is 1.6mL and 18.4mL of the bacteria solution are uniformly mixed), the concentration of the dhm1, the dhm2 and the dhm3 reach the highest value, respectively reach 0.95 g/plant, 1.10 g/plant and 0.98 g/plant, and the fresh weight of the corn seedlings cultivated by the three strains reaches the highest value of 1.27 g/plant. The liquid fermentation medium control has lower growth promoting effect than the strain combination of the fermentation of the adding bacteria, which indicates that active substances with growth promoting effect on corn seedlings are produced in the fermentation process.
Strain inoculation proportion optimization:
the inoculation was performed in the order of dhm1, dhm2 and dhm3, the total inoculum size was 100. Mu.L (0.2% of the shake flask liquid amount), and the respective inoculum sizes of the three strains were set to (10. Mu.L+40. Mu.L+50. Mu.L), (10. Mu.L+10. Mu.L+80. Mu.L), (30. Mu.L+50. Mu.L+20. Mu.L), (20. Mu.L+40. Mu.L), (20. Mu.L+20. Mu.L+60. Mu.L), (10. Mu.L+45. Mu.L), (20. Mu.L+10. Mu.L+70. Mu.L) and (30. Mu.L+35. Mu.L), respectively, and the other fermentation culture methods, cultivation of corn seedlings and root irrigation were the same. After 14d of corn seedling culture, the influence of different inoculation volume ratios of 3 strains on the growth of corn seedlings is measured.
TABLE 3 influence of the Compound Strain on fresh weight of maize seedlings
As is clear from Table 3, the three-strain composite fermentation was carried out at a ratio of (dhm 1: dhm2: dhm 3) of 10:40:50 to the highest, the total weight of the corn seedlings was 2.20 g/strain, the fresh weight of the stems and leaves was 1.12 g/strain, and the fresh weight of the roots was 1.08 g/strain. The second is the compounding ratio of 10:10:80, 20:40:40 and 10:45:45, and the total weight of the seedlings is 1.84 g/plant, 1.83 g/plant and 1.83 g/plant respectively.
And (3) disease prevention effect measurement:
1. determination of inhibition effect of single strain on corn stem rot
(1) Activating the strain: and (3) carrying out streak culture on the 3 strains by adopting a plate colony streak method to obtain single colonies.
(2) Seed liquid preparation: the lawn is respectively picked up and inoculated into a test tube filled with 8mL of liquid fermentation medium, and then the test tube is placed in a shaking table for 15h to obtain seed liquid.
(3) Adjusting OD value of seed liquid: the density of the bacterial liquid is regulated by a spectrophotometer to obtain OD 600 Bacterial suspension=1.0 was used for seed liquid for fermentation culture inoculation.
(4) And (3) carrying out shaking fermentation culture: the seed solution with the adjusted OD value is inoculated into a 150mL triangular flask filled with 50mL of culture medium with 0.2% of inoculation amount, and the mixture is placed in a shaking table for culturing for 72 hours to prepare fermentation liquor.
(5) Preparing fermentation supernatant: and (3) centrifuging the fermented bacterial liquid by a shaking table at 10000rpm at 4 ℃ for 10min to obtain a fermentation supernatant, and storing the fermentation supernatant in a sterilized reagent bottle for later use.
(6) Film sterilization and activity determination: filtering the centrifuged supernatant with 0.22 μm filter membrane, measuring the inhibitory activity of fermentation supernatant produced by different strains on bacteria by using a filter paper sheet method (taking the center of a poured flat plate as the center of a circle, taking 2cm around the center of the circle, 4 points which are mutually perpendicular, sequentially placing round filter paper sheets with the diameter of 7mm on the four points, finally dripping the prepared bacteria on the paper sheets by using a pipetting gun, and measuring the antibacterial bandwidth after culturing for a certain time).
TABLE 4 inhibition of Fusarium zematokii by dhm1, dhm2, dhm3
As is clear from Table 4, the antibacterial efficiency of the strain dhm2 was measured by the filter paper method.
2. Determination of inhibition effect of strain compounding on corn stem rot
Inoculation was performed in the order of dhm1, dhm2, and dhm3, the total inoculum size was 100. Mu.L, the volume of the liquid fermentation medium was 50mL, the inoculum sizes of the three strains were set to (10. Mu.L+40. Mu.L+50. Mu.L), (10. Mu.L+10. Mu.L+80. Mu.L), (30. Mu.L+50. Mu.L+20. Mu.L), (20. Mu.L+40. Mu.L), (20. Mu.L+20. Mu.L+60. Mu.L), (10. Mu.L+45. Mu.L), (20. Mu.L+10. Mu.L+70. Mu.L), and (30. Mu.L+35. Mu.L), respectively, and the antibacterial bandwidths of the different combinations were determined by referring to the filter paper sheet method described above.
TABLE 5 inhibition effect of the compounded (dhm 1: dhm2: dhm 3) strains on corn stem rot
As can be seen from Table 5, the inoculation ratio of the dhm1, the dhm2 and the dhm3 has the best bacteriostasis effect in a ratio of 10:40:50, and the bacteriostasis bandwidth is 8.60mm; secondly, the inoculation ratio of 20:20:60 and 30:40:30 is that the bacteriostasis bandwidth reaches 6.50mm.
Preparation of organic fertilizer:
1. planting, harvesting, airing and crushing the sweet potatoes
Selecting varieties suitable for planting in different areas, taking Mao She seeds and the photic vetch as main planting varieties, and sowing in the late 9 months to the early 10 months to various winter idle fields, orchards, tea gardens and other winter non-sowing land blocks. The cultivation and management of the vetch are simple, the nitrogen is naturally fixed, and no other fertilizer is generally required to be applied. Selecting sunny days for harvesting more than three consecutive days before and after the initial flowering period of the sweet potatoes in the month of April in the next year, and airing in time after harvesting. After the sweet potatoes are fully aired, the sweet potatoes are crushed and bagged by a crusher and then are moved to a shady and dry place for storage for standby.
2. Preparation of organic fertilizer
Preparation of functional bacteria diluent: and (3) inoculating the dhm1, the dhm2 and the dhm3 into a liquid fermentation culture medium according to an inoculation volume ratio of 10:40:50 (the inoculation volume ratio is 0.2%), placing the liquid fermentation culture medium in a shaking table for culturing for 72 hours to prepare functional bacterial liquid, and then adding water into the functional bacterial liquid to dilute the functional bacterial liquid to obtain the functional bacterial diluent.
Preparation of Trichoderma culture: respectively inoculating the dhm4 strain and the dhm5 strain stored on the inclined plane to a trichoderma culture medium plate, and culturing until trichoderma grows on the whole plate; then inoculating into a bottle filled with corn kernels (sterilized at high temperature and high pressure), and culturing until trichoderma grows over the whole bottle; inoculating the corn kernels full of trichoderma (subjected to high-temperature high-pressure sterilization) into a barrel filled with the corn kernels, and culturing until the trichoderma grows full of the barrel; and finally uniformly mixing the dhm4 and the dhm5 corn grain culture in the mass ratio of 4:1 to obtain the culture.
Primary fermentation time: 15-20d in summer, 25-30d in spring and autumn and 35-40d in winter.
Secondary fermentation time: 10-15d in summer, 20-25d in spring and autumn and 30-35d in winter.
Table 6 raw materials for preparing organic fertilizer
Potted plant promotion effect of organic fertilizer on corn and watermelon:
1. influence of organic fertilizer on corn growth
The effect of the organic fertilizer prepared above on the growth of corn and watermelon was determined by pot experiments. The test is carried out in the 6 th month of 2021, a flowerpot with the height of 18cm and the upper caliber of 20cm is selected, a layer of newspaper is placed on the bottom, then 100g of the prepared organic fertilizer is weighed, 2.0kg of dried soil and a filling material consisting of a matrix according to the proportion of 2:1 are added above the organic fertilizer, 6 corn hybrid seeds are sowed in each pot after water is poured, the pot is placed in open air for cultivation, natural rotten sweet potatoes and the prepared matrix are used as a contrast, and 6 pots are repeated for contrast and treatment. And (3) after the corn grows to a period of 3 leaves and 1 heart, keeping 2 robust seedlings in each pot, continuously culturing, growing the seedlings for 30 days, and drying and weighing dry weight. Sowing 4 watermelon seeds with germination accelerating and white exposing into the prepared flowerpot in the same way, selecting 2 healthy seedlings for continuous culture in the seedling stage, and drying and weighing dry weight after 30d of seedling emergence growth. And (3) in the growth process of the corns and the watermelons, watering the corns and the watermelons in time according to weather conditions.
TABLE 7 Effect of organic fertilizers on corn dry weight
As can be seen from table 7, the prepared organic fertilizer has the following growth promoting effects on corn from large to small: (1) the dry weight of the upper part of the land is dbs-2> dbs-5> dbs-1> dbs-3> dbs-4; (2) dry weight of underground part: dbs-5> dbs-1 ]
dbs-2> dbs-4> dbs-6; (3) the total weight is as follows: dbs-2> dbs-5> dbs-1> dbs-3> dbs-4. As can be seen from the growth promoting effect of the prepared 9 organic fertilizers on the corn, dbs-1, dbs-2 and dbs-5 have better growth promoting effect on the corn, and have better utilization value no matter the dry weight of the overground part, the dry weight of the underground part and the total dry weight are listed as the first three. From the viewpoint of saving cost (corn kernel cost), the utilization value of two organic fertilizers dbs-1 and dbs-2 is higher.
TABLE 8 influence of organic fertilizers on the dry weight of watermelons
As can be seen from table 8, the prepared organic fertilizer has the following growth promoting effects on watermelons (flowers are picked in the flowering period, and the watermelon is controlled not to sit) from large to small: (1) dry weight of aerial parts: dbs-1> dbs-8> dbs-2> dbs-4> dbs-5; (2) dry weight of underground part: dbs-2> dbs-5> dbs-3 and dbs-6> dbs-1; (3) the total weight is as follows: dbs-1> dbs-8> dbs-2> dbs-4> dbs-5. As can be seen from the growth promoting effect of the prepared 9 organic fertilizers on the watermelons, dbs-1, dbs-2 and dbs-5 have better growth promoting effect on the watermelons, and have better utilization value no matter the dry weight of the overground part, the dry weight of the underground part and the total dry weight of the overground part are listed in the first five. From the viewpoint of saving cost (corn kernel cost), the two organic fertilizers dbs-1 and dbs-2 are higher in utilization value.
2. Prevention and control effects of organic fertilizer on corn stalk rot and watermelon fusarium wilt
The prevention and control effects of the prepared organic fertilizer on corn stem rot and watermelon fusarium wilt are measured through a potting test. The test is carried out in 8 months of 2022, a flowerpot with the height of 18cm and the upper caliber of 20cm is selected, a layer of newspaper is filled on the bottom of the flowerpot, 40g of cultured germ (corn stalk rot: fusarium graminearum (Fusarium graminearum)) corn grain culture, 20g of watermelon fusarium oxysporum watermelon specialization (Fusarium oxysporum f.sp.niveum) corn grain culture are placed on the lowest layer of the flowerpot, 100g of the prepared different organic fertilizers are covered on the germ, then 2.0kg of soil and matrix are filled above the organic fertilizers to form a filling material with the composition of 2:1, 6 corn seeds are sown per pot after the watering, natural rotten sweet potato seeds, soil and matrix (2:1) are arranged, germ contrast and soil and matrix (2:1) blank contrast are added, and the different contrast and treatment are repeated for 6 pots. And (3) after the corn grows to a period of 3 leaves and 1 heart, keeping 2 robust seedlings in each pot for continuous culture, and drying and weighing dry weight after the seedlings grow for 50 days. And similarly, sowing 4 watermelon seeds with germination accelerating and white exposure into the prepared flowerpot, wherein the inoculation treatment method of germs is the same as that of corn, and observing and recording the death condition of the watermelon. And (3) in the growth process of the corns and the watermelons, watering the corns and the watermelons in time according to weather conditions.
Table 9 effect of organic fertilizers on growth and development of maize with stem rot on inoculated maize
As shown in Table 9, since Fusarium graminearum, which is a weak parasitic bacterium, was placed at the bottom of the pot, and the maize was in a period of stronger disease resistance when the maize root system was pricked down to the bottom of the pot, it was not generally lethal to maize hybrids, but had a greater effect on the growth of maize. As can be seen from the bacteria adding control and the blank control, the corn growth is inhibited to a certain extent after bacteria adding, however, after the prepared organic fertilizer is added, the weight of the overground parts of most of the organic fertilizer is larger than that of the bacteria adding control, wherein dbs-1> dbs-5> dbs-3; the weight of the underground part is dbs-8> dbs-1> dbs-3; the total weight is dbs-1> dbs-3> dbs-5. It can be seen that the dbs-1 organic fertilizer has the best comprehensive prevention and control effect on corn stem rot.
Table 10 effects of organic fertilizers on controlling watermelon fusarium wilt
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As shown in Table 10, the prepared organic fertilizer has the best effect of dbs-2 on cucumber fusarium wilt prevention and control, the lowest incidence rate is 28.57 percent, and the incidence rates of dbs-4 and dbs-8 are more than or equal to 60 percent after the other fertilizer types are applied. From the above study, dbs-1 has remarkable disease prevention and promotion effects on corns, and dbs-2 has good disease prevention and promotion effects on watermelons.
The two organic fertilizers dbs-1 and dbs-2 promote the effect of cucumber and spinach:
1. fruit of cucumber
The test was performed at 2022, 9 and 20. The fertilizing amount of each pot of the two organic fertilizers dbs-1 and dbs-2 is respectively 50 g/pot, 75 g/pot, 100 g/pot, 125 g/pot, 105 g/pot, 175 g/pot and 200 g/pot. Firstly, putting 0.4kg of matrix at the bottom of the flowerpot, then adding organic fertilizer, then adding 0.3kg of matrix, and watering properly. The cucumber seeds are firstly placed into a constant temperature and humidity incubator at 26 ℃ for germination, and are directly sown into a flowerpot after the seeds germinate, 1 plant/pot is repeated for 3 times. After seeding, the seeds are put into a constant temperature and humidity incubator (light and dark alternate culture, illumination for 16h,40000lx, setting 26 ℃ for 8h, setting 22 ℃) for culturing for 40d, and watering according to the soil humidity condition.
TABLE 11 influence of dbs-1 and dbs-2 organic fertilizers on total dry weight of cucumber
As can be seen from Table 11, the effect of dbs-2 organic fertilizer on cucumber growth promotion is superior to that of dbs-1 fertilizer, wherein the total dry weight of dbs-2 organic fertilizer is up to 2.85 g/plant at 150 g/pot, and the total dry weight of dbs-1 fertilizer is up to 2.46 g/plant at 175 g/pot.
2. Promoting effect on spinach
The test was performed at 10/11/2022. The fertilization amounts of dbs-1 and dbs-2 organic fertilizers per pot were 0.05kg,0.075kg,0.1kg,0.125kg,0.15kg,0.175kg and 0.2kg, respectively. Firstly, putting 0.4kg of matrix at the bottom of the flowerpot, then adding the quantitative organic fertilizer, then adding 0.3kg of matrix, and watering properly. The commercially available small leaf spinach seeds are firstly placed into a constant temperature and humidity incubator at 24 ℃ for germination, and after the spinach seeds germinate, the small leaf spinach seeds are directly sown into a flowerpot, 10 spinach seeds/pot are planted, and the first fertilization amount is repeated for 3 times. After seeding, the seeds are put into a plastic greenhouse for growing for 40 days, and watering is performed at proper time according to the soil humidity.
TABLE 12 influence of dbs-1 and dbs-2 organic fertilizers on total dry weight of spinach
As can be seen from Table 12, the total dry weight of the spinach of the soil control was 0.24 g/plant, and the total dry weight of the spinach showed a tendency of rising and falling with increasing fertilization amount of two organic fertilizers dbs-1 and dbs-2. The fertilizing amount of dbs-1 organic fertilizer is 150 g/pot, the total dry weight of spinach has maximum effect on promoting growth, and the total dry weight of spinach reaches 0.65 g/plant, which is 2.71 times of that of a blank control; the fertilizing amount of dbs-1 organic fertilizer is 100 g/pot, the total dry weight of spinach has maximum effect on promoting growth, and the total dry weight of spinach reaches 0.79 g/plant, which is 3.29 times of that of a blank control. Therefore, the two organic fertilizers dbs-1 and dbs-2 have obvious promotion effect on the total dry weight of the spinach, and the promotion effect of dbs-2 is more obvious.
Organic fertilizer replacing fertilizer potting test:
the prevention and control effects of the prepared organic fertilizer on corn stem rot and watermelon fusarium wilt are measured through a potting test. The test is carried out in 8 months and 20 days of 2022, a flowerpot with the height of 18cm and the upper caliber of 20cm is selected, a layer of newspaper is placed on the bottom of the flowerpot, 100g of prepared organic fertilizer (wherein dbs-1 organic fertilizer is selected for corn and dbs-2 organic fertilizer is selected for watermelon) is placed on the lowest layer of the flowerpot, then 2.0kg of a mixture composed of soil and matrix in a ratio of 2:1 is filled above the organic fertilizer, and 6 corn seeds are sown in each pot after water is permeated. When the corn seedlings grow to 3 leaves and 1 core period, 2 healthy seedlings are reserved in each pot, the cultivation is continued, the application amount of each pot of compound fertilizer (nitrogen, phosphorus and potassium ternary compound fertilizer, 18 (nitrogen) -18 (phosphorus pentoxide) -18 (potassium oxide)) is respectively 2g, 4g, 6g, 8g and 10g, the application is divided into 2 times of digging holes and applying (three leaves are fertilized for 1 time in one core period, the fertilization amount is the same after one week, the fertilization amount is the 2 times), the treatment of only applying compound fertilizer is additionally arranged, the fertilization amount is respectively 4g, 6g, 8g, 10g and 12g, and each treatment is repeated for 6 pots. And (5) drying and weighing the dry weight after the corn seedlings grow for 40d. Sowing 4 watermelon seeds with germination accelerating and white exposing into the prepared flowerpot, fertilizing for 1 time in the seedling stage, and fertilizing for 2 times in the vine stretching stage, wherein the fertilizing treatment method is the same as that of corn, and the watermelon is dried and weighed after 40d of emergence growth, and is mechanically watered according to weather conditions in the growth process of the watermelon and the corn.
Table 13 influence of partial replacement of fertilizers by dbs-1 organic fertilizers on corn growth
As can be seen from Table 13, when the amount of the compound fertilizer applied is 4 g/pot, the total dry weight of the corn is only 35.42g, and the total dry weight of 100g+4g of the dbs-1 organic fertilizer reaches 42.50g, which indicates that the dbs-1 organic fertilizer and the compound fertilizer can obviously promote the growth of the corn. The corn growth rate (42.30 g) of the dbs-1 organic fertilizer 100g+2g compound fertilizer can be equivalent to that of 8g compound fertilizer (42.13 g), and the potential of replacing the chemical fertilizer by the dbs-1 organic fertilizer can be seen to be larger. The test observation can also prove that the drought resistance of the corn can be obviously improved after the dbs-1 organic fertilizer is added.
Table 14 influence of partial replacement of chemical fertilizers by dbs-2 organic fertilizers on watermelon growth
As can be seen from Table 14, when the compound fertilizer application amount is 4 g/pot, the total dry weight of the watermelons is only 10.48g, and the total dry weight of the watermelons of 100g+4g compound fertilizer of dbs-2 organic fertilizer reaches 17.62g, which shows that the dbs-2 organic fertilizer and compound fertilizer can obviously promote the growth of the watermelons. The growth amount (12.63 g) of the watermelon of the 100g+2g compound fertilizer of the dbs-2 organic fertilizer is between 6 and 8g compound fertilizer (11.40 to 13.59 g) which is independently applied, so that the dbs-2 organic fertilizer has higher potential for replacing the fertilizer.
And (3) field application:
1. prevention and control effect on corn stalk rot and influence on corn yield
The test is arranged at a radar mountain corn disease resistance identification base of Anhui academy of technology, and corn stem rot pathogen-Fusarium graminearum is inoculated in 13 leaf period of corn every year from 2007, so that the residual pathogen quantity in soil is large, and an artificial disease nursery is formed. The test is carried out at 2022, 6 and 15 days, land preparation is carried out before corn sowing, and the corn row spacing is set to be 60cm, the plant spacing is set to be 24.7cm, and the plant spacing is set to be 4500 plants/mu. The dbs-1 organic fertilizer is firstly ditched and applied as a base fertilizer according to the fertilizing amount of 400 kg/mu, the compound fertilizer (nitrogen, phosphorus and potassium ternary compound fertilizer, 18 (pure nitrogen) -18 (phosphorus pentoxide) -18 (potassium oxide)) is applied along the sowing ditch according to the fertilizing amount of 80 kg/mu, then the corn hybrid is sown, and the compound fertilizer is applied according to the fertilizing amount of 80 kg/mu instead of the organic fertilizer. Treatments and controls were repeated 3 times for 0.5 mu each. Harvesting at 10 months and 7 days, and investigating the corn stem rot one week before harvesting, wherein investigation methods refer to the corn disease and insect resistance identification technical specification (NY/T148.7-2016).
TABLE 15 influence of dbs-1 organic fertilizers on corn stalk rot and yield
As shown in Table 15, the yield of the dbs-1 organic fertilizer and the compound fertilizer is 354.5kg, the reduced yield is 709.0 kg/mu, and the incidence rate is 15.0%; the control yield was 200.3kg, which was 400.6 kg/mu, with a 40.0% incidence. Therefore, the dbs-1 organic fertilizer can reduce the incidence of stem rot and remarkably improve the yield of corn.
2. Influence on prevention and control effect of watermelon fusarium wilt, watermelon yield and sweetness
The test is arranged in a greenhouse for planting watermelons in successive years in a planting science and technology garden of Anhui academy of science and technology, and the disease of watermelon fusarium wilt is serious due to successive cropping. And (3) starting to prepare soil and field planting melon seedlings in the last 3 ten days of 2022, ridging, cultivating, ditching, applying dbs-2 fertilizer, applying the prepared dbs-2 organic fertilizer according to the fertilizing amount of 400 kg/mu, and field planting melon seedlings according to the plant spacing of 0.5m and the row spacing of 2 m. After the seedlings are alive, urea is applied according to the proportion of 5 kg/mu, when the vines grow to 40-50cm, urea is applied according to the fertilizing amount of 15 kg/mu, and potassium sulfate is applied according to the proportion of 10 kg/mu, so that the growth of the vines is promoted. When the first melon grows to the egg size and the fuzz is removed, a compound fertilizer of 30 kg/mu is applied, the fertilizer application mode is hole application, and the comparison is that no dbs-2 organic fertilizer of 400 kg/mu is applied, and the other treatments are the same as the above. Control and treatment were repeated 3 times each, 0.1 mu each. The yield was weighed at harvest, sweetness was measured using a sweetness meter, and morbidity was calculated.
Table 16 Effect of dbs-2 organic fertilizers on watermelon fusarium wilt and yield
As shown in Table 16, the dbs-2 organic fertilizer has a larger promoting effect on the yield of watermelons, can reach 150.8kg, and has a reduced yield of 1508 kg/mu; the yield is reduced by 42.1% due to the heavy wilt of the control, which is only 87.3kg, and the yield is 873 kg/mu; the sweetness of the watermelon is increased by 3.8 degrees Bx, and the incidence rate is reduced by 29.1 percent. Therefore, the dbs-2 organic fertilizer can reduce the incidence rate of blight, remarkably improve the yield of watermelons and increase the sweetness of watermelons.
The foregoing has outlined and described the basic principles, features, and technical effects of the present invention. It will be understood by those skilled in the art that the present invention is not limited by the foregoing examples, which are provided by way of illustration of the principles of the present invention, and that various changes and modifications (including changes in the ratio of functional bacteria dilutions and trichoderma cultures added, changes in the primary and secondary fermentation times, etc.) may be made without departing from the spirit and scope of the invention, and such changes and modifications fall within the scope of the claimed invention. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (6)
1. A composite strain, characterized in that: the composite strain is prepared from bacillus amyloliquefaciensBacillus amyloliquefaciens) Bacterial strain dhm1 and bacillus beziacillusBacillus velezensis) Bacterial strain dhm2, bacillus subtilisBacillus subtilis) Bacterial strain dhm3, trichoderma harzianumTrichoderma harzianum) Strain dhm4 and trichoderma asperellumTrichoderma asperellum) Strain dhm 5;
the preservation number of the strain dhm1 is CCTCC NO: m20221589;
the preservation number of the strain dhm2 is CCTCC NO: m20221590;
the preservation number of the strain dhm3 is CCTCC NO: m20221591;
the preservation number of the strain dhm4 is CCTCC NO: m20221588;
the preservation number of the strain dhm5 is CCTCC NO: m20221587.
2. A microbial inoculum, characterized in that: the microbial inoculum comprises the composite strain of claim 1.
3. Use of the composite strain of claim 1 in the preparation of organic fertilizer.
4. An organic fertilizer, characterized in that: the organic fertilizer takes the vetch as a fermentation carrier and adopts the composite strain as the fermentation strain as claimed in claim 1.
5. A preparation method of an organic fertilizer is characterized by comprising the following steps: inoculating the composite strain of claim 1 onto a fermentation carrier by a secondary fermentation method.
6. Use of the organic fertilizer of claim 4 or the organic fertilizer obtained by the preparation method of claim 5 in crop planting.
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