AU2020104315A4 - A method for improving saline-alkali tolerance of maize seedlings and improving physical and chemical properties of rhizosphere soil of maize seedlings - Google Patents
A method for improving saline-alkali tolerance of maize seedlings and improving physical and chemical properties of rhizosphere soil of maize seedlings Download PDFInfo
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Abstract
The invention discloses a method for improving the saline-alkali tolerance of maize seedlings
and improving the physical and chemical properties of the rhizosphere soil of maize seedlings.
The invention protects the application of Klebsiella variicola in any of the following (al)-(a6): (al)
Promoting maize growth; (a2) Promoting maize growth under saline-alkali environment; (a3)
Increasing enzyme activity of maize rhizosphere soil; (a4) Improving enzyme activity of maize
rhizosphere soil under saline-alkali environment; (a5) Improving saline-alkali tolerance of maize.
(a6) The saline-alkali tolerance of maize was improved by increasing soil enzyme activity of
maize rhizosphere under saline-alkali environment. The invention uses exogenous beneficial
microorganisms in the cultivation process of maize seedlings, improves the saline-alkali tolerance
of maize seedlings, avoids the residue and enrichment of chemical components, and meets the
needs of the development of green agriculture. In addition, this method is very safety, and suitable
for popularization or transformation, also has great significance for improving the root
microecological environment and promoting the growth of maize seedlings under saline-alkali
stress.
Description
A method for improving saline-alkali tolerance of maize seedlings and improving physical and chemical properties of rhizosphere soil of maize seedlings Technical field:The invention relates to a method for improving the saline-alkali tolerance of maize seedlings and improving the physical and chemical properties of the rhizosphere soil of maize seedlings. Background technology: The area of saline-alkali land in China is huge, which seriously limits the development of planting industry. Maize was affected deeply as a crop sensitive to saline-alkali components. How to improve the rhizosphere soil microenvironment of maize especially at seedling stage and improve the saline-alkali tolerance of maize seedlings will be of great significance to the sustainable development of agriculture in saline-alkali areas. For a long time, people often use the method of chemical regulation to improve the saline-alkali tolerance of maize seedlings, which has serious chemical residues, deteriorates the soil environment, and is not conducive to the sustainable development of agriculture. In recent years, with the development of molecular biology, the research on improving the saline-alkali tolerance of maize seedlings by molecular breeding technology is gradually increasing, but the safety of some molecular breeding methods, such as transgenic technology, is controversial and the prospect remains to be examined. Invention content: The object of the invention is to provide a method for improving the saline-alkali tolerance of maize seedlings and improving the physical and chemical properties of the rhizosphere soil of maize seedlings. In the first aspect, the invention first protects the application of Klebsiella variicola in any of the following (al)-(a6): (al) Promoting maize growth; (a2) Promoting maize growth under saline-alkali environment; (a3) Increasing enzyme activity of maize rhizosphere soil; (a4) Increasing enzyme activity of maize rhizosphere soil under saline-alkali environment; (a5) Improving saline-alkali tolerance of maize; (a6)Improving saline-alkali tolerance of maize by increasing enzyme activity of maize rhizosphere soil under saline-alkali environment. In the second aspect, the invention protects the application of the combination of Klebsiella variicola and Bacillus licheniformis in any of the following (al)-(a6): (al) Promoting maize growth; (a2) Promoting maize growth under saline-alkali environment; (a3) Increasing enzyme activity of maize rhizosphere soil; (a4) Increasing enzyme activity of maize rhizosphere soil under saline-alkali environment; (a5) Improving saline-alkali tolerance of maize; (a6)Improving saline-alkali tolerance of maize by increasing enzyme activity of maize rhizosphere soil under saline-alkali environment. In the first and second aspects above, the purpose of promoting maize growth is to increase plant height and / or aboveground dry weight and / or underground dry weight and / or grain weight and / or yield of maize; the increase of enzyme activity of maize rhizosphere soil under saline-alkali environment is to increase the activities of urease and / or alkaline phosphatase and/ or sucrase and / or catalase in maize rhizosphere soil under saline-alkali environment. The grain weight can be 100 grain weight. The yield can be specific to the yield under 14% water content. In the second aspect mentioned above, the bacteria content ratio of Klebsiella variicola and Bacillus licheniformis is 0.5-3: 1. The ratio of bacteria content of Klebsiella variicola and Bacillus licheniformis can be 0.5:1 (1:2 in the embodiment), 1:1, 2:1 or 3:1. Among them, the best choice is 2:1. Third, the invention protects any of the following bacterial agents; Bacterial agent A: contains Klebsiella variicola; Bacterial agent B: contains Klebsiella variicola and Bacillus licheniformis. The bacterial agent A may contain Klebsiella variicola of 1 X 102cfu/ml-1 X 10 cfu/ml. More specifically, the bacterial agent A may contain 1 X 102cfu/ml, 1 X 10 4 cfu/ml, 1 X 106cfu/ml (optimal) or 1 X 108 cfu/ml Klebsiella variicola. The bacterial agent B can contain 1 X 102cfu/ml-1 X 10 cfu/ml Klebsiella variicola and Bacillus licheniformis, and the bacterial content ratio of Klebsiella variicola and Bacillus licheniformis is 0.5-3: 1. More specifically, the bacterial agent B may contain 1 X 106cfu/ml of Klebsiella variicola and Bacillus licheniformis. The ratio of bacteria content of Klebsiella variicola and Bacillus licheniformis can be 0.5:1 (1:2 in the example), 1:1, 2:1 or 3:1. Among them, the most preferred one is 2:1. Fourth, the invention protects the application of the bacterial agent described in the third aspect in any of the following (al)-(a6): (a1) Promoting maize growth; (a2) Promoting maize growth under saline-alkali environment; (a3) Increasing enzyme activity of maize rhizosphere soil; (a4) Increasing enzyme activity of maize rhizosphere soil under saline-alkali environment; (a5) Improving saline-alkali tolerance of maize; (a6)Improving saline-alkali tolerance of maize by increasing enzyme activity of maize rhizosphere soil under saline-alkali environment. In the fourth aspect mentioned above, the purpose of promoting maize growth is to increase maize plant height and / or aboveground dry weight and / or underground dry weight and / or grain weight and / or yield; the improvement of enzyme activity of maize rhizosphere soil under saline-alkali environment is to increase urease and / or alkaline phosphatase and / or sucrase and/ or catalase activity of maize rhizosphere soil under saline-alkali environment. The grain weight can be 100 grain weight. The yield can be specific to the yield under 14% water content. Fifth, the invention protects method A or method B or method C; Method A, in order to promote the growth of maize under saline-alkali environment, includes the following steps: applying any of the following (bl)-(b3) to maize root or rhizosphere soil; Method B, in order to improve the enzyme activity of maize rhizosphere soil under saline-alkali environment, it includes the following steps: applying any of the following (b)-(b3) to maize root or rhizosphere soil; Method C is a method to improve the saline-alkali tolerance of maize by increasing the enzyme activity of maize rhizosphere soil under saline-alkali environment, which includes the following steps: applying any of (bl)-(b3) to maize root or rhizosphere soil; (bl) Klebsiella variicola; (b2) Klebsiella variicola and Bacillus licheniformis; (b3) the aforementioned bacterial agents.
In the fifth aspect mentioned above, the purpose of promoting maize growth is to increase plant height and / or aboveground dry weight and / or underground dry weight and / or grain weight and / or yield of maize; the improvement of enzyme activity of maize rhizosphere soil under saline-alkali environment is to increase the activities of urease and / or alkaline phosphatase and / or sucrase and / or catalase of maize rhizosphere soil under saline-alkali environment. The grain weight can be 100 grain weight. The yield can be specific to the yield under 14% water content. When Klebsiella variicola and Bacillus licheniformis are used in combination, the specific bacterial content ratio of Klebsiella variicola and Bacillus licheniformis can be 0.5:1 (1:2 in the example), 1:1, 2:1 or 3:1. Among them, the most preferred one is 2:1. In the above method, when the maize is cultivated in pot, any one of (bl)-(b3) can be applied to the soil after sowing, and the specific application dose can be 60ml bacterial suspension or bacterial agent per 300g soil; the bacterial concentration of the bacterial suspension or bacterial agent is 1 X 102cfu/ml~1 X 10cfu/ml(preferably 1 X 106 cfu/ml) In the above method, when the maize is cultivated in the field, any one of (bl)-(b3) can be applied to the soil after sowing, and the application dose can be 150ml bacteria suspension or bacterial agent; the bacterial concentration of the bacterial suspension or bacterial agent is 1 X 102 cfu/ml I X 10 8 cfu/ml (preferably 1 X 106 cfu/ml). Any of the above-mentioned Klebsiella variicola can be Klebsiella variicola from the China General microbial species Preservation and Management Center, numbered as CGMCC1.15640. Any of the above-mentioned Bacillus licheniformis can be a Bacillus licheniformis from the China General microbial species Preservation and Management Center, numbered CGMCC1.7677. Any of the above-mentioned saline-alkali environment can be a natural saline-alkali soil, and the natural saline-alkali soil can be a natural saline-alkali soil with pH9.2 and Na+ content 0.906 g kg-. Any of the above-mentioned maize can be Xianyu 335 maize. The invention has the following advantages: (1) The saline-alkali tolerance of maize seedlings is significantly improved under saline-alkali stress, and the aboveground dry weight, underground dry weight and plant height are significantly increased. The activities of urease, alkaline phosphatase, sucrase and catalase in the rhizosphere soil of maize seedlings were significantly increased. (2) Use of live bacteria and no chemical components such as fertilizers and pesticides to avoid environmental pollution and in line with the needs of development of green agriculture. (3) The strain can colonize in the soil for a long time and has strong persistence, which will have a beneficial effect on the planting of maize for many years in the future. The invention uses exogenous beneficial microorganisms in the cultivation process of maize seedlings, improves the saline-alkali tolerance of maize seedlings, avoids the residue and enrichment of chemical components, and meets the needs of the development of green agriculture. In addition, this method has high safety, is suitable for popularization or transformation, and has great significance for improving the root microecological environment and promoting the growth of maize seedlings under saline-alkali stress. Illustration with drawings Figure 1 shows the statistical results of urease activity, alkaline phosphatase activity, sucrase activity and catalase activity of maize in example 1. Figure 2 shows the results of phenotypic observation of maize in control and experimental group in example 1. Figure 3 shows the statistical results of urease activity, alkaline phosphatase activity, sucrase activity and catalase activity of maize in example 2. Specific implementation mode The following embodiments facilitate a better understanding of the invention but do not limit the invention. The experimental methods in the following embodiments are conventional methods unless otherwise specified. The test materials used in the following embodiments, unless otherwise specified, are purchased from conventional biochemical reagent stores. In the quantitative tests in the following embodiments, three repeated experiments are set, and the results are averaged. Klebsiella variicola: China ordinary microbial strain Preservation and Management Center, CGMCC1.15640. Bacillus licheniformis: China ordinary microbial strain Preservation and Management Center, CGMCC1.7677. Xianyu 335 maize: purchased from Heilongjiang Fuzun Agricultural Comprehensive Service chain Co., Ltd. LB liquid medium: peptone lOg, yeast extract powder 5g, sodium chloride Og, distilled water IL, pH 7.0. Example 1. Klebsiella variicola was used to improve the saline-alkali tolerance of maize seedlings and improve the physical and chemical properties of rhizosphere soil of maize seedlings. I. Preparation of bacterial suspension Klebsiella variicola was inoculated in LB liquid medium and cultured at 30 C and 180rpm until the bacterial suspension containing sufficient bacteria was obtained. The bacterial suspension was centrifuged at 6000r • min for 15 minutes, and the supernatant was discarded after centrifugation. The precipitation was washed with aseptic distilled water and re-suspended, repeated for 3 times, the bacterial suspension was prepared into1 X 10cfu/ml respectively, and then diluted to 1 X 102cfu/ml, 1 X 104cfu/ml, 1 X 106cfu/ml respectively according to the needs of the experiment, ready for use. II. Maize sprouting treatment Select Xianyu 335 maize seeds, select maize seeds of the same size and no damage on the surface, soak and disinfect them with sodium hypochlorite (concentration 10%) for 10 minutes, and then wash them with sterile water for more than 5 times until there is no obvious taste of sodium hypochlorite. Soak the sterilized seeds in sterile water and put them in a constant temperature incubator for 6 hours, then discard the sterile water used. Spread a piece of filter paper with the same area as the bottom of the germination box and drop sterile water evenly, the amount of water can soak the whole filter paper slightly. The imbibed seeds were placed evenly in the germination box with 20 seeds in each box, and then a piece of filter paper was spread on the seeds and each piece of paper was evenly dripped with 3ml aseptic water. The germination box was covered with a lid and placed in a 25C incubator to accelerate germination in darkness for 24 hours. III.Pot preparation The potted soil was thoroughly air-dried and passed through a 2mm sieve. Each pot (specification: length X width X height is lOcm X lOcm X 12cm) was loaded with 200g soil. The potted soil is natural saline-alkali soil (pH9.2,Na+ content 0.906g kg'), which is taken from
Daqing City, Heilongjiang Province. The maize seeds with bud length of about lcm (0.9cm-1.lcm) 24 hours after germination in step 2 were planted in pots filled with soil, 5 seeds were evenly sown in each pot and 100 g of soil was covered on top of each pot. IV. Inoculated bacterial suspension Take the pot after covering the soil poured into 60ml different concentrations of bacterial suspensions obtained from step 1(when 60ml bacteria suspension was added to every 300g soil, the soil water content was suitable, which was consistent with that of conventional maize cultivation). The pots were cultured in a light incubator for 9 days, and 10ml sterile water was added to each pot every 48 hours during the culture period. The control group without adding bacterial suspension was set up. The culture conditions are as follows: the day and night temperature is 25C/ 20°C, the light is 12 hours a day, and the humidity is 60%-80%. After 9 days of culture, the plant height, aboveground dry weight and underground dry weight of maize seedlings were calculated, and the activities of urease, alkaline phosphatase, sucrase and catalase in rhizosphere soil of maize seedlings were calculated. Plant height measurement method: measured in the pot, select the maize seedlings with the same growth in each treatment, straighten the leaves, and use a ruler to measure the length from the soil surface to the top of the highest leaves. Measurement method of aboveground dry weight: the maize seedlings with the same growth in each treatment were cut off at the junction of roots and stems, the stems and leaves were put into the oven, baked at 80C to constant weight, and the aboveground dry weight was weighed. The method of measuring the dry weight of the underground part: take the maize seedlings with the same growth in each treatment, cut the maize seedlings at the junction of the rhizome, take the roots into the oven, bake to a constant weight at 80°C, and weigh the dry weight of the underground part. The detection methods of urease activity, alkaline phosphatase activity, sucrase activity and catalase activity refer to the literature: Guan Songyin. Soil enzyme and its research method [M]. Agricultural Press, 1986. The results are shown in figure 1, figure 2, and Table 1. The results showed that the plant height, aboveground dry weight and underground dry weight of maize seedlings increased significantly (P < 0.05) after 9 days of culture. When the bacterial concentration reached 106cfu/ml, each index basically reached (close to) the maximum value, and continued to increase the bacterial concentration when the bacterial concentration 10 8cfu/ml, each index did not significantly increase compared with the bacterial concentration 106cfu/ml, so combined with economic cost and effectiveness and other reasons, 106cfu/ml was determined to be the most suitable addition. Compared with the control group (CK) without adding bacterial suspension, the plant height increased by 38.11%, the aboveground dry weight increased by 79.76%, and the underground dry weight increased by 72.22%. The activities of various enzymes in the rhizosphere soil of maize seedlings increased significantly (P < 0.05), urease activity increased by 64.22%, alkaline phosphatase activity increased by 61.90%, sucrase activity increased by 50.26%, and catalase activity increased by 49.21%.
Table Concentration Plant height(cm) Aboveground dry weight (g) Underground dry weight(g) (cfu/ml)
CK 23.220+1.264d 0.084+0.005d 0.054+0.004c
102 26.390+0.586c 0.097+0.007c 0.058+0.003c
104 29.973+1.094b 0.128+0.007b 0.078+0.005b 106 32.070+0.947a 0.151+0.002a 0.093+0.003a 108 32.603±0.699a 0.153+0.004a 0.094+0.002a
The above results showed that the use of Klebsiella variicola could significantly improve the root microecological environment and promote the growth of maize seedlings under saline-alkali stress. Example 2. The combination of Klebsiella variicola and Bacillus licheniformis was used to improve the saline-alkali tolerance of maize seedlings and improve the physical and chemical properties of rhizosphere soil of maize seedlings. I. Preparation of bacterial suspension Klebsiella variicola was inoculated in LB liquid medium and cultured at 30 C and 180rpm until the bacterial suspension containing sufficient bacteria was obtained. The bacterial suspension was centrifuged at 6000r • min for 15 minutes, and the supernatant was discarded after centrifugation. The precipitation was washed with aseptic distilled water and re-suspended, repeated for 3 times, the bacterial suspension was prepared into 1 X 10cfu/ml respectively. Bacillus licheniformis was inoculated in LB liquid medium and cultured at 30V and 180rpm until the bacterial suspension containing sufficient bacteria was obtained. The bacterial suspension was centrifuged at 6000r min- for 15 minutes, and the supernatant was discarded after centrifugation. The precipitation was washed with aseptic distilled water and re-suspended, repeated for 3 times, the bacterial suspension was prepared into 1 X 10cfu/ml respectively. The Klebsiella variicola suspension (concentration 1 X 106cfu/ml) and Bacillus licheniformis suspension (concentration 1 X 106cfu/ml) were mixed according to different volume ratios to obtain the following bacterial suspension (the total volume of each bacterial suspension was ml): Bacterial suspension (1:0): Klebsiella variicola suspension with a concentration of I X cfu/ml. 60ml suspension contains about 6 X 10cefu Klebsiella variicola. Bacterial suspension (3:1): 3 volume of Klebsiella variicola suspension (1 X 106 cfu/ml) and 1 volume of Bacillus licheniformis suspension (1 X 106cfu/ml) were mixed. 60ml suspension contains about 4.5 X 107 cfu Klebsiella variicola and 1.5 X 107 cfu Bacillus licheniformis. Bacterial suspension (2:1): 2 volume of Klebsiella suspension (I X 106 cfu/ml) and 1 volume of Bacillus licheniformis suspension (1 X 106 cfu/ml) were mixed. 60ml suspension contains about 4X 10 7 cfu Klebsiella variicola and about 2X 107 cfu Bacillus licheniformis. Bacterial suspension (1:1): 1 volume of Klebsiella suspension (I X 106 cfu/ml) and 1 volume of Bacillus licheniformis suspension (1 X 106cfu/ml) were mixed. 60ml suspension contains about 3X 10 7 cfu Klebsiella variicola and about 3X 107 cfu Bacillus licheniformis. Bacterial suspension (1:2): 1 volume of Klebsiella suspension (I X 106 cfu/ml) and 2 volume of Bacillus licheniformis suspension (1 X 106 cfu/ml) were mixed. 60ml suspension contains about 2X 10 7 cfu Klebsiella variicola and about 4X 107 cfu Bacillus licheniformis.
Bacterial suspension (0:1): Bacillus licheniformis suspension with a concentration of 1 X 106cfu/ml. 60ml suspension contains about 6 X 10cfu Bacillus licheniformis. II. Maize sprouting treatment Select Xianyu 335 maize seeds, select maize seeds of the same size and no damage on the surface, soak and disinfect them with sodium hypochlorite (concentration 10%) for 10 minutes, and then wash them with sterile water for more than 5 times until there is no obvious taste of sodium hypochlorite. Soak the sterilized seeds in sterile water and put them in a constant temperature incubator for 6 hours, then discard the sterile water used. Spread a piece of filter paper with the same area as the bottom of the germination box and drop sterile water evenly, the amount of water can soak the whole filter paper slightly. The imbibed seeds were placed evenly in the germination box with 20 seeds in each box, and then a piece of filter paper was spread on the seeds and each piece of paper was evenly dripped with 3ml aseptic water. The germination box was covered with a lid and placed in a 25C incubator to accelerate germination in darkness for 24 hours. III.Pot preparation The potted soil was thoroughly air-dried and passed through a 2mm sieve. Each pot (specification: length X width X height is 1Ocm X 1Ocm X 12cm) was loaded with 200g soil. The potted soil is natural saline-alkali soil (pH9.2,Na+ content 0.906g kg-), which is taken from Daqing City, Heilongjiang Province. The maize seeds with bud length of about lcm (0.9cm-1.lcm) 24 hours after germination in step 2 were planted in pots filled with soil, 5 seeds were evenly sown in each pot and 100 g of soil was covered on top of each pot. IV. Inoculated bacterial suspension Take the pot after covering the soil poured into 60ml different concentrations of bacterial suspensions obtained from step 1(when 60ml bacteria suspension was added to every 300g soil, the soil water content was suitable, which was consistent with that of conventional maize cultivation). The pots were cultured in a light incubator for 9 days, and 10ml sterile water was added to each pot every 48 hours during the culture period. The culture conditions are as follows: the day and night temperature is 25C/ 20°C, the light is 12 hours a day, and the humidity is 60%-80%. After 9 days of culture, the plant height, aboveground dry weight and underground dry weight of maize seedlings were calculated, and the activities of urease, alkaline phosphatase, sucrase and catalase in rhizosphere soil of maize seedlings were calculated. The results are shown in table 2 and figure 3. The results showed that through the optimization of the addition ratio of Klebsiella variicola and Bacillus licheniformis, the best ratio of Klebsiella variicola: Bacillus licheniformis was 2: 1, the plant height was 33.267cm, the aboveground dry weight was 0.160g, and the underground dry weight was 0.101g. The activities of various enzymes in the rhizosphere soil of maize seedlings were significantly increased (P < 0.05), urease, alkaline phosphatase, sucrase and catalase were 0.717mg / kg, 0.832mg / g, 31.077mg / g and 2.794mg/g respectively.
Table 2 Add Proportion Plant height(cm) Aboveground dry Underground dry
(Klebsiella variicola: weight (g) weight(g) Bacillus licheniformis) 1:0 32.070+0.947b 0.151+0.002b 0.093+0.003b
3:1 32.003+0.137b 0.150+0.003b 0.096+0.002b
2:1 33.267+0.509a 0.160+0.004a 0.101+0.004a 1:1 30.980+1.11lb 0.132+0.006c 0.081+0.002c 1:2 26.717+0.491c 0.100+0.008d 0.059+0.002d 0:1 26.093+0.188c 0.091+0.004e 0.055+0.003d
Example 3. Field experiment I.The location of the experiment The experimental practice base of Heilongjiang Bayi Agricultural University, the soil is saline-alkali soil (pH8.9,Na+ content 0.891g kg-). II.The time of the experiment Two batches of experiments were carried out. The first batch of sowing time is May 25, 2018, and the harvest time is October 6, 2018. The second batch of sowing time: May 20, 2019, harvest time: October 3, 2019. III.Experimental method 1. Before sowing, the plot is uniformly carried out conventional continuous field operations such as rotary ploughing and stubble killing, raking after turning, fertilization, ridging, suppression and so on. 2. The operation was carried out according to the following groups: The control group (CK): Sowed Xianyu 335 maize seeds, added 150ml water to the soil during sowing, and then covered with 200g soil above the sowing place. Experimental group (TI): Sowing Xianyu 335 maize seeds, added 150ml Klebsiella variicola suspension (1 X 106cfu/ml ) as shown in example 2 to the soil during sowing, and then covering the soil with 200g above the sowing site. Experimental group (T2): Sowing Xianyu 335 maize seeds, added 100ml Klebsiella variicola suspension (I X 106cfu/ml ) and 50ml Bacillus licheniformis(1 X 106 cfu/ml ) as shown in example 2 to the soil during sowing, and then covering the soil with 200g above the sowing site. The addition amount of water or bacterial suspension in the above groups was determined by pre-experiment. When the addition amount was 150ml, the bacterial liquid could reach the 30cm below the surface, and the reaching range was consistent with the root growth range of maize at seedling stage. Each of the above-mentioned processing has 6 lines, each length is 20m, width is 0.6m, and each process is repeated 3 times. The field management methods of the above-mentioned treatment groups were the same. 70kg of nitrogen fertilizer was applied in jointing stage and big trumpet stage respectively according to the conventional method, and the seedlings were carried out according to the conventional method in the two-leaf stage of maize, and irrigated according to rainfall and soil water content in the field. 3. Three 30m2 samples were taken from each treatment during harvest, and the number of effective panicles in the sample was checked. After artificial threshing, the fresh grain weight and moisture content were measured, and the yield under 14% water content was calculated. Ten ears were selected for seed testing in each treatment group, and the number of rows per ear, grains per row and 100-grain weight were measured. The results are shown in Table 3. The results showed that the application of Klebsiella variicola in maize cultivation could increase the yield of maize in saline-alkali soil. Table 3 Year Processing Number of rows Number of grains 100-grain weight Yield (kg/hm 2
) group per ear per row (g)
CK 14.4c 27.9c 31.42c 9579.17c 2018 TI 15.9b 32.8b 36.47b 10073.50b
T2 16.5a 33.9a 37.73a 10737.33a CK 15.Oc 28.2c 32.06c 9692.67c 2019 TI 16.4b 33.2b 37.2b 10931.23b
T2 17.3a 34.5a 38.15a 11400.33a
Claims (10)
1. A composition for improving the saline-alkali tolerance of maize seedlings and/or improving physical and chemical properties of rhizosphere soil surrounding maize seedlings, said composition comprising an effective amount of Klebsiella variicola.
2. The composition of claim 1, wherein the composition is effective in increasing enzyme activity within the rhizosome soil of one or more enzymes selected from the group consisting of urease, alkaline phosphatase, sucrase and catalase.
3. The composition of claim 1 or 2 further comprising an effective amount of Bacillus licheniformis.
4. The composition of claim 3, wherein the ratio of K. variicolato B. licheniformis present in the composition is 0.5-3 : 1.
5. The composition of claim 3, wherein the ratio of K. variicolato B. licheniformis present in the composition is 2 : 1.
6. The composition of claim 1 or 2, wherein the composition comprises 1 x 102 cfu/ml to 1 x 10 cfu/ml of K. variicola.
7. The composition of claim 6, wherein the composition comprises 1 x 10cefu/ml of K. variicola.
8. The composition of any one of claims 4 to 7, wherein the composition comprises a total of 1 x 106 cfu/ml of K. variicola and B. licheniformis.
9. The composition of any one of claims 1 to 8, wherein the composition is a bacterial suspension.
10. A method of applying the composition of claim 9 to soil within a pot sown with maize, wherein the composition is applied to the soil in an amount of 60 ml per 300g soil.
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5 i
5 '• 3 I•
0.9 0.8
ii in 0.7
iiiiii 0.6
5 0.5
f 0.4 1 s0-3 - 0.2
0.1
0.0 1:0 3:1 21 1:1 12 0:1 10 3:1 2:1 1:1 1-2 ai Add PiopoitionKlebsiella vamcda Bacillus hchemforms) Add Proportion ( Klebsiella vanicola: Bacillus lichenifomis) 2020104315
3.0
35
lilm llll 30
3= 25 v 20 f 1 15 i 10 I 5
0 ™ i：i i：2 o.i 00 1 B B B B U Add Propoition ■ Klebsiella vanicola: Bacillus lichenifomis) 1:0 3:1 2:1 1:1 1:2 0:1 Add Proportion ( Klebsiella vanicola: Bacillus licherafcrnis)
Table 3
2/2
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| CN117004540B (en) * | 2023-09-28 | 2023-12-15 | 北京量维生物科技研究院有限公司 | Screening and application of Klebsiella variabilis azotobacter |
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