CN116218709A - Endophytic Burkholderia gladioli JS-59 and application thereof - Google Patents
Endophytic Burkholderia gladioli JS-59 and application thereof Download PDFInfo
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- CN116218709A CN116218709A CN202211621659.XA CN202211621659A CN116218709A CN 116218709 A CN116218709 A CN 116218709A CN 202211621659 A CN202211621659 A CN 202211621659A CN 116218709 A CN116218709 A CN 116218709A
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Abstract
The invention relates to an endophyte Burkholderia gladioli JS-59 and application thereof, wherein the endophyte Burkholderia gladioli (Burkholderia gladioli) JS-59 is obtained by separating and purifying from medicinal chrysanthemum plants, and is preserved in China Center for Type Culture Collection (CCTCC) for 11 months and 29 days in 2022, the preservation number is CCTCC NO: M20221844, and the preservation address is the China center for type III, wuchang district, eight-way Wuhan university in Wuhan, the city of Wuhan, hubei province. The bacteria have antibacterial activity on various important plant pathogenic fungi such as rhizoctonia cerealis, wheat take-all and the like in wheat production, are broad-spectrum microbial agents/bacterial fertilizers with good development and application prospects, and have important significance on green prevention and control of diseases of wheat, rice, corn, apples, cotton, rape and tobacco.
Description
Technical Field
The invention belongs to the technical field of agricultural microorganisms, and particularly relates to an endophytic burkholderia gladioli (Burkholde ria gladioli) JS-59 for biological control of plant diseases.
Background
The plant disease is caused by pathological changes in aspects of morphology, physiology and biochemistry and the like under the influence of biological or non-biological factors in the growth and development processes of the plant, so that the normal growth and development of the plant are hindered, and the economic benefit is further influenced. Common plant disease control methods include chemical control, physical control, biological control, disease-resistant breeding, and the like. With the importance of sustainable agriculture and environmental protection in China, biological control means are increasingly used for plant disease and pest control. The biological control of plant diseases is to effectively control crop diseases by using beneficial microorganisms and microbial metabolites, wherein the biological control of plant diseases by using biocontrol bacteria is a hot spot of current research.
Wheat is one of four main foods in the world and is also an important grain crop in China. The wheat in China has wide distribution area, and most provinces are planted. At present, the wheat sowing area of China is between 3 and 4 hundred million mu each year, and the wheat yield of China reaches 13434 ten thousand tons in 2021 year according to the report of the national statistical bureau. However, wheat is subjected to biotic stress during growth, where infestation by various pathogenic bacteria can cause significant yield losses. The wheat sheath blight and wheat take-all disease are common diseases in wheat production, and according to the data of the department of agriculture, the annual incidence area of the wheat sheath blight and the wheat take-all disease in China reaches tens of thousands of mu, and the disease is very serious in a wheat producing area in more than one hundred million mu in some years.
Wheat sheath blight and wheat take-all are typical soil-borne diseases, and control is very difficult. At present, chemical agent prevention and control are mainly adopted in production, but the utilization of bactericides brings about unsafe environment caused by residues and can increase the generation of the drug resistance risk of pathogenic bacteria. And because of the large-area planting of wheat dwarf Xuan multi-spike varieties, the fertilizing amount is increased and the like, the banded sclerotial blight of wheat and the wheat take-all have obvious increasing trend in many areas, and the healthy production and quality of wheat crops in China are seriously threatened.
In recent years, research and utilization of beneficial microorganisms have been increasingly emphasized, and development of low-toxicity and environment-friendly biogenic bacterial manure/microbial agents is particularly important for healthy development and disease prevention and control of the wheat industry. Endophytes are biological flora which exist in various tissues and organs of plants and have no obvious harm to the plants and are stably symbiotic with host plants, and generally have the effects of promoting plant growth, helping the plants resist external stress and the like, and are important components of a plant micro-ecological system. The endophyte can enhance the resistance of a plant host to pathogenic organisms, so that the protection of the host can be realized to a great extent, and the host is prevented from being damaged and infringed. Therefore, the method for screening the microbial strains with high efficiency, low toxicity, high selectivity and environmental friendliness is an effective method for solving the problem that the current wheat bactericides influence the environmental safety by utilizing microorganisms with bactericidal activity. According to reports, bacillus cereus, bacillus subtilis, pseudomonas, paenibacillus terrestris, trichoderma and the like which have a certain prevention and treatment effect on the wheat sheath blight at present. CN202211029288.6 discloses a strain of biocontrol bacteria against banded sclerotial blight of wheat, identified as pseudomonas orientalis;
CN201510315978.1 discloses that screening from a wild rice plant of the same species of burkholderia gladiolus rice sheath blight germ, gibberella wheat germ, sclerotinia gracilis, sclerotinia sclerotiorum, sesame fusarium wilt and pepper root rot germ has antibacterial effect, can be applied to control of rice sheath blight disease, wheat scab, plantain sclerotinia sclerotiorum, rape sclerotinia, sesame fusarium wilt and pepper root rot, but the strain mainly has the effect of promoting plant growth, has weak antibacterial capability and antibacterial capability of only 30% -40%. Screening more effective microorganism has positive significance for crops such as wheat and the like.
Disclosure of Invention
In order to solve the problem of obtaining a microbial strain capable of efficiently inhibiting plant fungal diseases such as wheat sheath blight, wheat take-all and the like, the inventor obtains a strain which can better meet the requirements through extensive screening and testing and finally separating and purifying in medicinal chrysanthemum plants, and therefore, the inventor provides the following technical scheme:
in one aspect, the invention provides a Burkholderia gladioli strain which is a Burkholderia gladioli (Burkholderia gladioli) JS-59 and has been preserved in China Center for Type Culture Collection (CCTCC) on the 11 th month 29 of 2022, wherein the preservation number is CCTCC NO: M20221844, and the preservation address is the eight-channel university of Wuchang district in Wuhan, hubei province. In one aspect, the invention provides application of the selected burkholderia gladioli strain in preventing and treating plant fungal diseases.
In one aspect, the invention provides application of the selected burkholderia gladioli strain in preparation of a biological agent for preventing and treating plant fungal diseases.
Preferably, the plant fungal diseases to which the present invention relates are fungal diseases of wheat, rice, maize, apple, cotton, canola and tobacco.
Preferably, the plant fungal diseases to which the present invention relates are Rhizoctonia cerealis, strawberry anthracnose, tomato gray mold, cotton blight, apple rot, apple ring rot, rice bakanae disease, curvularia lunata leaf spot, corn small spot, rape sclerotinia rot, rhizoctonia cerealis, rice blast, tobacco brown spot and tobacco black shank.
In one aspect, the present invention provides a biological agent comprising Burkholderia gladioli JS-59 or a metabolite thereof. The Burkholderia gladioli JS-59 can be used for preparing bacterial liquid for the production of biological agents through large-scale fermentation culture. The bacterial solution of burkholderia gladioli JS-59 can also be separated to obtain metabolites thereof, which are believed to have the same control effect as the fermentation bacterial solution.
Preferably, the biological agent comprises a liquid agent, freeze-dried powder agent and immobilized agent. The liquid preparation can be in the form of common microbial pesticides such as suspension, liposome liquid, emulsion and the like; the lyophilized powder is also a commonly used preparation form, which can be conveniently transported and used, and can efficiently protect the activity of thalli in a lyophilized form, and of course, the lyophilized powder can also contain lyoprotectants and stabilizers such as trehalose, proteins and the like which are well known in the art. The immobilized preparation is prepared by immobilizing microorganisms in a porous carrier, wherein the porous carrier comprises diatomite, active carbon, porous silica and the like, and the immobilized preparation has the advantage of keeping the activity of the microorganisms for a long time to prevent the microorganisms from being impacted by the outside.
In one aspect, the present invention provides a method for controlling plant fungal diseases using a biological agent by applying a biological agent comprising Burkholderia gladioli JS-59 to a subject plant to prevent or inhibit plant fungal diseases. Preferably, the biological agent of the present invention can also be used together with other agents for controlling plant fungal diseases to enhance its effect.
Advantageous effects
1. The biological preparation JS-59 of Burkholderia gladioli has wide bacteriostasis spectrum, has bacteriostasis activity on various important plant pathogenic fungi such as sheath blight of wheat and take-all of wheat in wheat production, is broad-spectrum microbial agent/bacterial fertilizer with good development and application prospect, and has important significance on green prevention and control of diseases of wheat, rice, corn, apples, cotton, rape and tobacco.
2.The biological preparation of the Burkholderia gladioli JS-59 has the advantages of simple culture condition, high propagation speed and easy preservation and transportation.
3. The Burkholderia gladioli JS-59 is obtained by separating and purifying from healthy medicinal chrysanthemum plants, is safe and nontoxic, and is environment-friendly.
Drawings
Fig. 1: JS-59 Strain A: colony morphology B: gram staining
Fig. 2: physiological and biochemical characteristics of JS-59 strain
Fig. 3: antibacterial effect of endophyte JS-59on Rhizoctonia cerealis and wheat take-all, wherein A: a sheath blight germ control group; BC: test group D of banded sclerotial blight bacteria: wheat take-all germ control group; EF: wheat take-all germ test group.
Fig. 4: the Brucella gladiolus JS-59 phylogenetic tree.
Fig. 5: antibacterial effect of Burkholderia gladioli JS-59on various plant fungi.
Fig. 6: control effect of burkholderia gladioli JS-59on wheat sheath blight pot experiments, wherein A: a control group; b: burkholderia gladioli JS-59 strain treatment group.
Fig. 7: control effect of burkholderia gladioli JS-59on wheat take-all potting test, wherein A: a control group; b: burkholderia gladioli JS-59 strain treatment group.
Detailed Description
The invention is further described below in connection with specific embodiments. The technical scheme of the invention is a conventional mode in the field unless specifically stated, and the reagent or the material is a conventional reagent and is derived from commercial channels unless specifically stated.
Example 1 separation and purification of endogenous Burkholderia gladioli JS-59, preparation thereof, and determination of antibacterial Activity against Rhizoctonia cerealis and wheat take-all
1.1 preparation of Medium
PDA medium: 200.0g of potato, 20.0g of glucose, 20.0g of agar powder, 1.0L of distilled water and sterilizing for 20min at 121 ℃;
LB medium: 5.0g of yeast extract powder, 10.0g of tryptone, 5.0g of NaCl, 1.0L of distilled water and sterilizing for 20min at 121 ℃;
NA medium: 3.0g of beef powder, 10.0g of peptone, 5.0g of sodium chloride, 20.0g of agar powder, 1.0L of distilled water, 7.2 of PHS and sterilization at 121 ℃ for 25min.
Wheat grain sand culture medium 1000mL, sand 500mL and glucose 20g; the wheat grains are soaked in water for 12 hours and boiled for 1 hour, and then the wheat grains are uniformly mixed with sand and glucose, and the mixture is sterilized by high-pressure steam at 121 ℃ for 1 hour.
1.2 isolation, purification and preparation of endophytic bacterium JS-59
Healthy medicinal chrysanthemum golden-silk chrysanthemums are selected from a comprehensive testing station of Annu, fuyang, linquan county, annu, new farm and Annu, northwest, and returned to a laboratory, the golden-silk chrysanthemums stalks are collected, washed and sheared into small sections of about 1cm, the small sections are sterilized on the surface of 22.5% sodium hypochlorite for 5min, soaked in 75% alcohol for 3min, and finally rinsed with sterile water for 5 times. The stems are naturally dried and put into a mortar, and 5ml of sterile water is added for grinding. Standing for 15min after grinding, respectively diluting the supernatant with mother liquor for 10 times, 100 times and 1000 times, respectively taking 0.1ml of diluted liquid to be coated on LB solid culture medium flat plate, carrying out three-region marking on the stock solution, and repeating each concentration for 3 times. Culturing in dark at 28deg.C for 24 hr. Single colonies were picked for purification according to colony morphology and the colony count per dish was calculated. Meanwhile, taking 0.1ml of sterile water for rinsing the sample for the last time as a plate coating control, repeating for 3 times, and if no colony grows out on the plate, indicating that the surface of the sample is thoroughly disinfected. And (3) picking single bacterial colonies on an LB plate, further purifying the single bacterial colonies on an LB culture medium plate by adopting a plate streaking method to obtain an endophytic bacterial strain JS-59, and preserving the endophytic bacterial strain JS-59 for later use. The antagonistic strain JS-59 obtained by screening was inoculated into NA medium, and after 48 hours of culture, the colony was round, smooth in surface and edge, milky white in color, and glossy in surface (A in FIG. 1). The results of gram staining of HB-10 strain showed that the cell was a gram-negative strain (B in FIG. 1).
The physiological and biochemical test results are shown in figure 2, and the JS-59 strain is positive for gelatin liquefaction, contact enzyme, glucose, sorbose, galactose, sorbitol, fructose, methyl red, V-P test and citrate utilization reaction. The bacterial strain is negative to the reactions of starch hydrolysis, tryptophan deaminase, sucrose, lactose, mannitol, oxidase, maltose, soluble starch, inositol and indole, and is judged to be the burkholderia strain by comprehensively observing the growth characteristics and morphological characteristics of the JS-59 strain.
TABLE 1 physiological and biochemical characteristics Tab1 The physiological and biochemical characteristics of strain JS-59 of strain JS-59
Note that: "+" indicates positive and "-" indicates negative
Note:“+”means positive,“－”means negative
The endophytic bacterial strain JS-59 is prepared into a biological agent through liquid fermentation, and the biological agent is specifically: the endophytic bacterial strain JS-59 is cultured in a shaking table at the constant temperature of 28 ℃ and 180r/min for 36 hours, and then the biological preparation bacterial liquid is obtained.
1.3 determination of antibacterial Activity of endophytic bacterium JS-59 against Rhizoctonia cerealis
Test target strain: rhizoctonia cerealis Rhizoctonia cerealis, wheat take-all Gaeumannomyces grami nis, offered by the university of Anhui agriculture plant protection college.
The antibacterial activity of the endophyte JS-59 is measured by adopting a plate counter method by taking the sheath blight germ and the wheat take-all germ as targets.
The primary screening of antagonizing endophytic bacteria is carried out by taking the sheath blight germ and the take-all germ of wheat as target bacteria. Inoculating pathogenic bacteria to the center of a PDA plate, symmetrically inoculating the separated strains at the position of 2.5cm of a bacterial cake, taking the plate inoculated with the pathogenic bacteria as a blank control, culturing in an inverted mode in a constant-temperature incubator at 25 ℃, and observing and recording the antibacterial effect of the strains on the pathogenic bacteria when the bacterial colony in the control grows to more than 2/3 of a dish. And re-screening the bacterial strain with the antibacterial effect. Inoculating pathogenic bacteria to the center of a PDA plate, inoculating the same primary screening strain at 2.5cm positions on two sides of a bacterial cake, taking the plate inoculated with the pathogenic bacteria as a blank control, culturing in an inverted mode in a constant temperature incubator at 25 ℃, measuring the lengths of the sheath blight bacteria and the wheat take-all bacteria by a crisscross method when the colony in the control grows to more than 2/3 of a dish, and calculating the inhibition rate of endophytic bacteria JS-59on the sheath blight bacteria.
The antibacterial effect of endophyte JS-59on Rhizoctonia cerealis and wheat take-all is shown in figure 3. The result shows that the endophytic bacteria JS-59 has strong inhibition effect on the hypha growth of the sheath blight germ and the take-all germ of wheat, and the inhibition rates are 80.94 percent and 89.50 percent respectively. The endophyte JS-59 can effectively inhibit the hypha growth of the target bacteria.
Example 2 molecular characterization of Endoconcha JS-59
2.1 preparation of Medium
The medium formulation was the same as 1.1 in example 1.
2.2 molecular characterization of endogenous bacteria JS-59
Extracting JS-59 strain genome DNA, and PCR amplification by taking the same as a template
Primer sequence: 27F (5'-AGAGTTTGATCCTGGCTCAG-3') and 1492R (5'-GGTTACCTTGTTACGA CTT-3') were subjected to 16SrRNA amplification.
PCR amplification System (25. Mu.L): 2 XTaqMastermix 12.5. Mu.L, 1. Mu.L of each 1. Mu. L, DNA template for the upstream and downstream primers, and 9.5. Mu.L of ddH 2O.
The PCR reaction conditions were: 94 ℃ for 3min;94℃30s,58℃ 30s,72℃60s,34 cycles; and at 72℃for 5min. The PCR products were detected by 1% agarose gel electrophoresis, amplified products were sequenced by Shanghai qing biological company, the obtained gene sequences were spliced and subjected to homology comparison analysis at NCBI (https:// blast. NCBI. Lm. Nih. Gov/blast. Cgi), strain sequence information with higher homology was downloaded, and a phylogenetic tree was constructed using MEGA7 software as shown in FIG. 4.
The results show that: 16S rRNA obtained by PCR amplification of the endophyte JS-59 has the highest homology of 99% with Burkholderia gladioli Burkholderia gladioli by NCBI comparison. The endophytic bacteria JS-59 are identified as Burkholderia tangutica Burkholderia gladioli, the Burkholderia tangutica JS-59 is preserved in China Center for Type Culture Collection (CCTCC) at the year of 11 and 29 of 2022, the preservation number is CCTCC NO: M20221844, and the preservation address is the eight-channel university collection of Wuchang district in Wuhan, hubei province.
Example 3 determination of antibacterial spectrum of endogenous bacteria JS-59
3.1 preparation of Medium
The medium formulation was the same as 1.1 in example 1.
3.2 test strains
Bacteriostasis spectrum test strain: pathogenic bacteria such as Botrytis cinerea, cotton fusarium wilt Fusarium oxysporium, apple rot Valsa mali, apple ring rot Physalospora piricola, rice bakanae disease Fusarium moniliforme, curvularia lunata leaf spot Curvularia lunata, curvularia lunata leaf spot Curvularia clavata, curvularia lunata Cochliobolus heterostrophus, sclerotinia sclerotiorum Sclerotinia sclerotiorum, magnaporthe oryzae, alternaria tabaci Alternaria alternata, rhizoctonia solani Rhizoctonia solani and tobacco black shank Phytophthora parasitica are provided by the university of Anhui agricultural plant protection institute.
3.3 bacteriostasis Spectrometry Using plate counter method
The procedure is as in example 1 at 1.3.
The antibacterial effect of endophyte JS-59on strawberry anthracnose, tomato gray mold, cotton blight, apple rot, apple ring rot, rice bakanae disease, rice curvularia leaf spot, corn small spot, rape sclerotinia, corn sheath blight, rice blast, tobacco brown spot and tobacco black shank pathogenic bacteria is shown in figure 5. The bacteriostasis spectrum measurement result shows that: the biological agent of the endophyte JS-59 has strong antibacterial effect and broad antibacterial spectrum, can strongly inhibit the growth of hyphae of the 14 tested pathogens, and the hyphae at the colony edge of the 14 pathogenic fungi contacted with the endophyte JS-59 grow sparsely or stop growing, for example, the hyphae of the sheath blight of corn is completely inhibited by the endophyte JS-59, and the inhibition rate of the endophyte JS-59on the plant diseases is shown in table2.
TABLE2 inhibition of various pathogenic fungi by JS-59 strain Table2.TheininbitionofstrenJS-59 onplantapathogeneicfurgi
Note that: different letters in the same column indicate difference significance (P < 0.05)
Example 4 potted plant control efficacy determination of endophytic bacterium JS-59
4.1 preparation of Medium
The medium formulation was the same as 1.1 in example 1.
4.2 potted plant control effect determination of endophytic bacterium JS-59
Plant material wheat variety: jimai 22.
Selecting full seeds, washing the seeds with tap water, soaking and sterilizing the seeds in 1% sodium hypochlorite solution for 10min, and washing the seeds with sterile water for 3-5 times. The Rhizoctonia cerealis and the wheat take-all are cultured and stored on a PDA culture medium according to a conventional method. Inoculating to PDA plate, culturing at 25deg.C for 7d, cutting PDA culture medium full of Rhizoctonia solani into pieces, placing into wheat grain sand culture medium, slightly shaking to embed pieces in the middle of grains, culturing in 25 deg.C incubator for 4-5d, and shaking triangular flask daily to facilitate mycelium growth for 20 d. Mixing the cultured pathogenic bacteria and sterilized sand soil according to a proper proportion. The endophytic bacteria adopts a soil irrigation method, and the specific method is as follows: the pathogenic bacteria and sterilized sand which are cultured on the wheat grain sand culture medium are mixed according to the proportion of 1:10, and mixing uniformly. The wheat seeds after germination acceleration are sown in flowerpots with the diameter of 7cm, 10 seeds are sown in each flowerpot, and five seeds are repeatedly treated. After 20 days of greenhouse culture, the sheath blight grade of wheat is investigated and the disease index and the control effect are calculated according to the following method after 20 days of inoculation of sheath blight of wheat.
0-the whole wheat plant is normal;
1-leaf sheaths at the base of the wheat plant stem present typical wheat sheath blight lesions but did not infect wheat stalks;
3-pathogenic bacteria invade the wheat stalks, but the width ring stalk direction of the disease spots does not exceed 1/2 of the whole circumference of the wheat stalks;
5-pathogenic bacteria invade the stalks, but the width of the disease spots which spread around the stalks accounts for 1/2-3/4 of the circumference of the whole wheat plant stalks; 7-pathogenic bacteria invade the wheat stalks, but the width of the disease spots surrounding the wheat stalks is more than 3/4 of the circumference of the whole wheat plant stalks, or the stalks are in obvious soft rot state;
9-wheat appears as dry booting and dry white ears.
The biocontrol effect of the JS-59 strain on the banded sclerotial blight of wheat in a potting test is shown as a graph 6, and the potting test result shows that the wheat leaf sheath part of a pathogenic bacteria treatment control group has a cloud-banded brown pattern, the root system and the stem base part are seriously blackened and rotten, the root system is underdeveloped, the disease is serious, the wheat plants of a treatment group which is pretreated by endogenous bacteria JS-59 and then is inoculated with the banded wilt of wheat by mixing soil grow well, the disease number and the disease degree are far less than those of the control group, and the relative control effect is 63.75%.
After inoculating wheat take-all pathogen for 20 days, the wheat take-all grade was investigated and the disease index and control effect were calculated according to the following method.
Stage 1: the brown area of the damaged root occupies less than 1/2 of the total area;
2 stages: the brown area of the damaged root occupies more than 1/2 of the total area, but no black spot exists;
3 stages: the brown area of the damaged root accounts for more than 1/2 of the total area, the root has small sections to be blackened, and the blackened part accounts for less than 1/4 of the total area; 4 stages: the brown area of the damaged root occupies more than 1/2 of the total area, the root has small sections to be blackened, and the total area of the blackened part occupies 1/4-1/2;
5 stages: the brown area of the damaged root occupies more than 1/2 of the total area, the root has a small section to be blackened, and the blackened part occupies 1/4-1/2 of the total area, but the root is not blackened completely;
stage 6: the roots are all blackened, but the plants do not die;
7 stages: root rot and plant death.
The biocontrol effect of the JS-59 strain on wheat take-all in a potting test is shown as figure 7, and the potting test results show that the wheat She Jin ground stalks of a pathogenic bacteria treatment control group are blackened, root systems and stalk bases are seriously blackened and rotten, the morbidity is serious, the wheat plants of the treatment group which are pretreated by endophyte JS-59 and then are inoculated with wheat take-all bacteria by mixing soil grow well, and the number and the degree of the morbidity plants are far smaller than those of the control group, and the relative control effect is 71.09%.
The foregoing is a further elaboration of the present invention in connection with the detailed description, and it is not intended that the invention be limited to the specific embodiments shown, but rather that a number of simple deductions or substitutions be made by one of ordinary skill in the art without departing from the spirit of the invention, should be considered as falling within the scope of the invention as defined in the appended claims.
Claims (10)
1. The burkholderia tangutica strain is characterized in that the strain is burkholderia tangutica (Burkholderia gladioli) JS-59, and is preserved in China Center for Type Culture Collection (CCTCC) on the 11 th month 29 th year 2022, wherein the preservation number is CCTCC NO: M20221844, and the preservation address is the eight-path university collection of Wuhan in the Chang area of Wuhan, hubei province.
2.The use of a burkholderia tangutica strain according to claim 1 for controlling plant fungal diseases.
3. Use of a burkholderia tangutica strain according to claim 1 for the preparation of a biological agent for controlling plant fungal diseases.
4. The use according to claim 2 or 3, wherein the plant fungal disease is a fungal disease of wheat, rice, maize, apple, cotton, canola and tobacco.
5. The use according to claim 4, wherein the plant fungal disease is banded sclerotial blight of wheat, take-all disease of wheat, strawberry anthracnose, tomato gray mold, cotton blight, apple rot, apple ring rot, rice bakanae disease, curvularia oryzae leaf spot, curvularia corn leaf spot, corn small spot, rape sclerotinia, corn banded sclerotial blight, rice blast, tobacco brown spot and tobacco black shank.
6. A biological preparation comprising the burkholderia gladioli JS-59 or a metabolite thereof according to claim 1.
7. The biologic of claim 6, wherein said biologic comprises a liquid formulation, a lyophilized powder, an immobilized formulation.
8. A method of controlling plant fungal diseases using the biological agent of claim 6, wherein the biological agent is applied to a test plant to prevent or inhibit plant fungal diseases.
9. The method of claim 8, wherein the plant fungal disease is banded sclerotial blight of wheat, take-all disease of wheat, strawberry anthracnose, tomato gray mold, cotton blight, apple rot, apple ring rot, rice bakanae disease, curvularia oryzae leaf spot, curvularia corn leaf spot, corn small spot, rape sclerotinia, corn banded sclerotial blight, rice blast, tobacco brown spot, and tobacco black shank.
10. The method of claim 8, wherein the plant is wheat, rice, corn, apple, cotton, canola, and tobacco.
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| JP2010047532A (en) * | 2008-08-22 | 2010-03-04 | Meiji Univ | Method and agent for controlling plant disease |
| CN105018371A (en) * | 2015-06-11 | 2015-11-04 | 江西师范大学 | Endophytic bacterial strain--Burkholderia gladioli Fse32 of Dongxiang wild rice and application thereof |
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| CN113337438A (en) * | 2021-06-17 | 2021-09-03 | 华南农业大学 | Efficient endogenous Burkholderia gladioli NSPE25 and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115851495A (en) * | 2022-09-07 | 2023-03-28 | 西北农林科技大学 | Biocontrol bacterium for preventing and treating wheat scab, application of biocontrol bacterium and biocontrol bacterium agent |
| CN115851495B (en) * | 2022-09-07 | 2024-05-24 | 西北农林科技大学 | Biocontrol bacterium for preventing and treating wheat scab, application thereof and biocontrol bacterium agent |
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| CN116218709B (en) | 2023-12-15 |
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