CN113337438A - Efficient endogenous Burkholderia gladioli NSPE25 and application thereof - Google Patents
Efficient endogenous Burkholderia gladioli NSPE25 and application thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of biological control of plant diseases, and relates to an endophytic Burkholderia gladioli (Burkholderia gladioli) NSPE25 and application thereof. The invention separates and screens an endophytic Burkholderia gladioli NSPE25 strain from healthy guava fruits, the strain is preserved in Guangdong province microorganism strain preservation center in 11-3 months in 2020, and the preservation number is GDMCC No: 61263. the strain can effectively inhibit the growth of hypha of the guava scab germs and can obviously reduce the occurrence of the guava scab; meanwhile, the antibacterial agent has strong antibacterial effects on apple sarcodon scabra, mango yellow meadow pathogen and citrus sarcodon scabra, and has a wide antibacterial spectrum, and the antibacterial rate can reach 98.05%; the strain can stably survive in guava, has strong environmental adaptability, and is safe and nontoxic; the biocontrol agent is simple to culture, can provide a new material for developing a high-efficiency microbial agent/bacterial fertilizer for the guava scab, also provides a new choice for safe and effective biological prevention and control of fruit tree diseases, and has good application prospect.
Description
Technical Field
The invention belongs to the technical field of biological control of plant diseases, and particularly relates to a high-efficiency endogenous Burkholderia gladioli NSPE25 and application thereof.
Background
Guava (Psidium guajava) is a special fruit with edible nutrition and medicinal efficacy, is planted in China Guangdong, Guangxi, Fujian, Hainan, Taiwan and other provinces, and has high economic value. Guava scab caused by Pestalotiopsis sp, seriously affects the quality and healthy production of guavas (Linzheng faithful, Zea's terfene. guavas disease (II.) agricultural world, 1996,159: 54-56.). Guava scab mainly damages fruits, and most of the scab is round and slightly convex. The surface of the scab is rough and has cracks, such as scabs; in the middle of the latter lesions, small black spots often appear (Keith L M, Vel' asquez ME, Zee FT, Identification and characterization of Pestalotiopsis sp. occupying scab disease of guava, Psidium guajava, in Hawaii. plant disease.2006,90: 16-23.). In the production process, the prevention and control of guava scab are mainly based on the prevention and control of medicaments, but the safety problem of fruits and vegetables caused by pesticide residues is more and more concerned by the general public.
The endophyte does not infect host plants or cause obvious disease symptoms, and lives in tissues and organs of healthy plants at a certain stage or whole stage in the life history, and is in reciprocal symbiosis with the host plants. The endophyte has important functions of resisting (plant diseases and insect pests, adversity and heavy metal) stress, promoting growth, inducing resistance and the like of host plants. Few reports are reported about the biological control of guava diseases, and the mining, the development and the utilization of endophyte resources have important significance for the safe and effective control of the guava diseases.
Although there are reports on biological control of Pestalotiopsis sp, for example, chinese patent CN112662578A published in 2021 at 4/16 discloses that paenibacillus polymyxa DX32 can effectively inhibit phytopathogenic fungi of Pestalotiopsis, but the strain has strong individual specificity and is greatly influenced by the environment as a pure culture. It is necessary to continuously excavate and explore new biocontrol bacteria for plant diseases with better control effect.
Disclosure of Invention
The invention aims to solve the technical problem of overcoming the defect of biological control of guava scab (caused by pestalotiopsis), and provides a high-efficiency endogenous Burkholderia gladioli NSPE25 and application thereof. The NSPE25 strain is separated from healthy guava fruits, and has remarkable inhibitory effect on guava scab; meanwhile, the NSPE25 strain has good inhibition effect on various plant fungal diseases.
The invention aims to provide a high-efficiency endogenous Burkholderia gladioli NSPE 25.
The invention also aims to provide the application of the high-efficiency endogenous Burkholderia gladioli NSPE25 in the aspect of preventing and treating plant fungal diseases, in particular the aspect of preventing and treating fruit tree fungal diseases.
The invention also aims to provide the application of the high-efficiency endogenous Burkholderia gladioli NSPE25 in the preparation of the biological preparation for preventing and treating the fungal diseases of fruit trees.
The invention also aims to provide a biological preparation containing endogenic Burkholderia gladioli NSPE 25.
The method for controlling the plant fungal diseases by using the biological preparation containing the endogenous Burkholderia gladioli NSPE25 also belongs to the protection scope of the invention.
The above object of the present invention is achieved by the following technical means:
an endophytic Burkholderia gladioli NSPE25, which is deposited in Guangdong province microorganism culture collection center at 11 months and 3 days 2020 and has the deposit number of GDMCC No: 61263, the preservation address is No. 59 building 5 of the No. 100 Dazhou prefecture of Guangzhou city, Guangdong province.
The strain is obtained by separating, purifying and screening healthy guava fruits collected from guava growing areas in southern sand areas of Guangzhou city, Guangdong province.
The experimental result shows that the endogenous Burkholderia gladioli NSPE25 has a strong bacteriostatic action on the hypha growth of the scab bacteria of the guava, and can obviously relieve the symptom of the scab bacteria of the guava (Pestalotiopsis sp. Pestalotiopsis sp.); meanwhile, the compound has strong inhibition effect on apple sarcodictyophora (Nectria cinnabarina red cluster scab), mango gummosis (Fusicoccum aesculi Chaetomium elegans) and citrus sarcodictyon (Neofusicoccum parvum novisporus), and the inhibition rate is 73.64-98.05%. The endophytic strain NSPE25 has good effect of preventing and treating fungal diseases of fruit trees, has a wide antibacterial spectrum, and has great application value and potential in the aspect of preventing and treating fungal diseases of plants. Therefore, the following applications are within the scope of the present invention.
The application of the Burkholderia endophytic NSPE25 in preventing and treating plant fungal diseases is provided.
The application of the Burkholderia endophytic NSPE25 in preparing biological preparation for preventing and treating plant fungal diseases is provided.
Preferably, the plant fungal disease is a fruit tree fungal disease.
More preferably, the fruit tree fungal disease is guava scab, apple coral leaf spot, mango tree gummosis or citrus fruit leaf spot.
Preferably, the plant fungus is pestalotiopsis, erythromyces cinnabarinus, cladosporium esculentum or fusarium novarum.
A biological agent containing endogenous Burkholderia gladioli NSPE25 is also within the protection scope of the invention.
Preferably, the biological agent comprises thallus and/or bacterial liquid of endogenous burkholderia gladioli NSPE 25.
Specifically, a biological agent containing endogenic Burkholderia gladioli NSPE25 is obtained by inoculating Burkholderia gladioli NSPE25 strain into liquid culture medium and culturing.
More specifically, the strain is prepared by inoculating Burkholderia gladioli NSPE25 to LB liquid culture medium with pH of 7.0, and culturing at 30-32 ℃ and 180-200 rpm for 48-60 h.
A method for controlling plant fungal diseases, which comprises using a biological preparation containing endogenous Burkholderia gladioli NSPE25 to control plant fungal diseases.
Preferably, the biological agent is endogenous Burkholderia gladioli NSPE25 serving as thalli and/or bacterial liquid thereof.
Preferably, the plant is a fruit tree.
Further preferably, the plant is guava, apple, mango or citrus.
Compared with the prior art, the invention has the following beneficial effects:
1. the endogenous Burkholderia gladioli NSPE25 biological preparation has a wide antibacterial spectrum, has antibacterial activity on various important plant pathogenic fungi harmful to scab germs and the like in fruit trees, is a broad-spectrum microbial preparation/bacterial fertilizer with good development and application prospects, and has important significance on green prevention and control of fruit tree diseases.
2. The endogenic gladiolus burkholderia NSPE25 biological preparation is simple and easy to prepare and convenient to popularize and apply.
3. The endophytic Burkholderia gladioli NSPE25 is obtained by separating and purifying healthy guava fruits, is safe and nontoxic, has good stability and is environment-friendly.
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FIG. 1 is a diagram showing the bacteriostatic effect of endophyte NSPE25 on guava scab, and FIG. 1-A shows that endophyte NSPE25 inhibits the hypha growth of guava scab; FIG. 1-B shows the colonies of the common scab fungus of guava.
FIG. 2 is a diagram showing the bacteriostatic effect of endophyte NSPE25 on Pleurotus eryngii, and FIG. 2-A shows that endophyte NSPE25 inhibits the hypha growth of Pleurotus eryngii; FIG. 2-B shows a colony of Chitosan cinnabarinus.
FIG. 3 is a diagram showing the bacteriostatic effect of endophyte NSPE25 on Fusarium aestivum, and FIG. 3-A shows that endophyte NSPE25 inhibits the hypha growth of Fusarium aestivum; FIG. 3-B shows a colony of Fusarium aestivum.
FIG. 4 is a diagram showing the bacteriostatic effect of the endophyte NSPE25 on Fusarium oxysporum, and FIG. 4-A shows that the endophyte NSPE25 inhibits the hypha growth of the Fusarium oxysporum; FIG. 4-B-bacterial colonies of Fusarium oxysporum.
Fig. 5 is a graph showing the effect of treatment with endophyte NSPE25 of the present invention on reducing the occurrence of guava scab symptoms, 5-a: NSPE25+ P (inoculating guava scab germ after processing guava fruit 1d by NSPE 25); 5-B: LB culture solution + P (inoculating guava scab germ after processing guava fruit for 1d by LB).
Detailed Description
The present invention is further illustrated by the following specific examples, which are not intended to limit the invention in any way. Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated.
Unless otherwise indicated, reagents and materials used in the following examples are commercially available.
Example 1 isolation, purification, identification and measurement of bacteriostatic Activity against Psidium guajava Burkholderia NSPE25
1.1 preparation of the culture Medium
NA medium: 10g of peptone, 5g of sodium chloride, 3g of beef extract and 20g of agar powder, and adding water to a constant volume of 1L; sterilizing at 121 deg.C for 20 min.
NB culture solution: 10g of peptone, 5g of sodium chloride and 3g of beef extract, and adding water to a constant volume of 1L; sterilizing at 121 deg.C for 20 min.
LB culture medium: 10g of peptone, 5g of sodium chloride, 5g of yeast extract and 20g of agar powder, and adding water to a constant volume of 1L. Sterilizing at 121 deg.C for 20 min.
PDA culture medium: cleaning and peeling potatoes, weighing 200g of potatoes, cutting into small pieces, adding water, boiling (boiling for 20-30 min), and filtering with eight layers of gauze. Adding glucose 20g, adding water to 1000mL, stirring, adding agar 15g, heating to dissolve completely, packaging into triangular flask, sterilizing at 121 deg.C for 20min, and storing.
1.2 isolation and purification of endophytic bacteria
In the guava growing area of south sand area of Guangzhou city, Guangdong province, healthy guava fruits are selected and brought back to the laboratory. The collected fresh healthy guava fruits are washed by water and dried in the air, 1g of guava peel is cut into a square shape of 10mm multiplied by 10mm, the guava peel is soaked in 75% alcohol for 1min and then soaked in 2% NaClO for 3min, the guava peel is washed with sterile water for 5 times, the guava peel is ground into paste by adding the sterile water, 200 mu L of the guava peel is coated on an NA plate, the culture is carried out at 28 ℃ for 3-4 d, single colonies with different forms are selected, the purified endophytic bacterial strains are further purified on a fresh LB culture medium plate by adopting a plate marking method, the purified endophytic bacterial strains are obtained, the bacterial strains comprise bacterial strains NSPE25, and the bacterial strains are stored for later use.
1.3 antibacterial activity assay of purified endophytic bacteria (including strain NSPE25) on Psidium guajava Leyss
Test target strains: the common scab of guava is a strain GZ1905 of Pestalotiopsis sp, and is provided by Bactericide research laboratory of Phytopathology department of southern China agricultural university.
And (3) inoculating the purified endophytic bacteria to an LB culture solution, and shaking the bacteria at 180rpm and 32 ℃ for 48h to obtain an activated bacteria solution.
And measuring the antibacterial activity of endophytic bacteria by using guava scab bacteria as a target and adopting a plate confrontation method. Activating guava scab bacterial strain GZ1905 on PDA plate, culturing for 6d, making into bacterial cake with diameter of 6mm with puncher, and inoculating in the center of new PDA plate; activated endophyte bacteria were inoculated into wells at a cross position 18mm from the cake, 4 wells per plate, 100 μ L/well. Control was inoculated with equal amount of LB medium. Dark culture at 28 ℃ for 6 days, biological repetition for three times, opposite culture and observation of colony growth, and taking blank control of inoculating guava scab bacteria only in the center of a PDA culture medium plate and inoculating LB culture solution in 4 holes. When the colony in the control grows to exceed 4/5 dishes, the colony area of the guava scab germs is measured, and the inhibition rate of the endophytic bacteria on the growth of the colony of the guava scab germs is calculated.
The result shows that the endophytic bacterium NSPE25 grows and spreads rapidly on the flat plate, has very strong inhibition effect on the hypha growth of the guava scab germ, and has the inhibition rate of 92.13%. The bacteriostatic effect of endophytic bacterium NSPE25 on the common scab of guava is shown in FIG. 1, and comparison of FIGS. 1A and 1B shows that endophytic bacterium NSPE25 can rapidly inhibit the growth of the hyphae of common scab, and the common scab hardly grows.
1.4 identification of endophytic bacterium NSPE25
1.4.1 morphological characterization
Respectively inoculating the purified endophytic bacterium NSPE25 to PDA and NA culture media, culturing for 24h at 36 ℃, and observing the morphology of colonies; and staining and morphological observation of the cells were performed using a gram stain solution kit (Qingdao Haibo Biotechnology Co., Ltd.). The result shows that the endophytic bacterium NSPE25 is a gram-negative bacterium and is short rod-shaped. The colony on the PDA culture medium is round and grey white, the size of a single colony is about 1mm, the surface is smooth and moist without folds, the center is raised, the edge is neat and opaque. The thallus on the NA culture medium is round and light yellow, the surface is smooth and slightly wet, and the thallus is raised and the edge is neat.
1.4.2 molecular characterization
The PCR amplification is carried out by using a bacterial solution of an endogenous bacterium NSPE25 as a template and a 16S rRNA gene universal primer fD2/rP 1. The primer sequence is as follows: fD2 (5'-AGAGTTTGATCATGGCTCAG-3') and rP1 (5'-ACGGTTACCTTGTTACGACTT-3'). The sequence of the 16S rDNA gene obtained by amplification is shown in SEQ ID NO. 1.
Sequence alignment by NCBI showed: the endophytic bacterium NSPE25 has the highest homology with Burkholderia gladioli (Burkholderia gladioli) of 99.67%. In conclusion, the endophytic bacterium NSPE25 belongs to Burkholderia (Burkholderia gladioli), and has been deposited in the Guangdong province collection center of microorganism strains at 11/3 of 2020 with the deposit number GDMCC No: 61263, the preservation address is No. 59 building 5 of the No. 100 Dazhou prefecture of Guangzhou city, Guangdong province.
1.5 preparation of endophytic bacterium NSPE25 biological agent
Inoculating an endophytic bacteria strain NSPE25 into LB culture solution with the pH value of 7.0, and culturing for 48-60 hours at the temperature of 30-32 ℃ at 180-200 rpm to obtain fermentation liquor, namely the biological agent.
Example 2 determination of bacteriostatic activity of endophytic bacterium NSPE25 on important pathogenic fungi of fruit trees
2.1 preparation of the culture Medium
The medium was prepared as in 1.1 of example 1.
2.2 test strains
Selecting test strains for determining the bacteriostatic spectrum of endophyte NSPE25 from 3 important pathogenic fungi in fruit tree production, wherein the test strains comprise: the bactericide is provided by the research laboratory of Phytopathology department of southern China agricultural university.
2.3 determination of other fruit tree fungal disease pathogenic bacteria by plate confronting method
The procedure was as in 1.3 of example 1.
The bacteriostatic effect of the endophyte NSPE25 on red bush roselle, cladosporium esculentum and new ascochyta parvum is shown in figures 2-4. The antibacterial spectrum measurement result shows that: the biological preparation of the endophytic bacterium NSPE25 has strong bacteriostatic action and wide bacteriostatic spectrum, and can be used for treating 3 test germs: the mycelial growth of the red bush roseum, the Eschschschschonoides schefflera and the Eschonospora parvula has strong inhibiting effect. As is clear from comparison of FIGS. 2A and 2B with FIGS. 3A and 3B, colonies of Chitosan cinnabarinus (2A) and Fusarium aestivum (3A) which confront the endophyte NSPE25 hardly grow; as is clear from comparison of FIGS. 4A and 4B, the hyphae of Fusarium novellum, which was in contact with the endophyte NSPE25, grew slowly and were difficult to spread. The endophyte NSPE25 has hypha growth inhibition rates of 98.05%, 86.79% and 73.64% on red bush red fungus, cladosporium esculentum and new ascochyta.
Example 3 determination of Effect of endophyte NSPE25 on the prevention and treatment of guava scab
3.1 preparation of the culture Medium
The medium was prepared as in 1.1 of example 1.
3.2 determination of preventive Effect of endophytic bacterium NSPE25
Guava variety: taiwan pearl.
The endophytic bacterium NSPE25 is inoculated to LB culture solution and shaken at 180rpm and 32 ℃ for 48h for standby. Selecting healthy and consistent guava fruits for inoculation, and adopting a perforation inoculation method. The method comprises the steps of quickly carrying out surface disinfection on healthy guava fruits by using 75% alcohol, washing the fruits clean under sterile water and naturally drying the fruits, and punching 2 holes in the equator of the fruits by using a puncher. After the wound was allowed to air dry, the treatment groups inoculated 200 μ L of 1X 10 concentration into the holes in the fruit9CFU·mL-1The control group is added with the same amount of LB culture solution into holes on the fruits. After the bacteria liquid/LB culture liquid is completely absorbed, placing the mixture in an incubator at 25 ℃ for 12 hours for moisture preservation, and then inoculating 150 mu L of the mixture with the concentration of 5 multiplied by 106each.mL-1The spore suspension of scab bacteria is observed at regular time, and the diameter of diseased spots of diseased fruits is measured by a cross method. The calculation formula is as follows:
after the scab germs are inoculated for 2 days, the fruits of the control group are inoculated in the holes for browning, the browning is carried out around the holes, the fruits quickly expand to the periphery to form brown large disease spots, and the fruits are rotten at the later stage (figure 5B); and half of the fruit inoculation holes inoculated with endophyte NSPE25 bacterial suspension in advance in the treatment group are browned, the periphery of the holes is browned slightly, the scab is only limited in the inoculation holes and is not expanded (figure 5A), and the prevention and treatment effect of the endophyte NSPE25 on the guava scab is 69.89%.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Sequence listing
<110> southern China university of agriculture
<120> high-efficiency endogenous Burkholderia gladioli NSPE25 and application thereof
<160> 3
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1496
<212> DNA
<213> Strain NSPE 2516S rDNA (Burkholderia gladioli)
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acggttacct tgttacgact tcaccccagt catgaatcct accgtggtga ccgtcctcct 60
tgcggttaga ctagccactt ctggtaaaac ccactcccat ggtgcgacgg gcggtgtgta 120
caagacccgg gaacgtattc accgcggcat gctgatccgc gattactagc gattccagct 180
tcatgcactc gagttgcagg gtgcaatccg gactacgatc ggttttctgg gattagctcc 240
ccctcgcggg ttggcgaccc tctgttccga ccattgtatg acgtgtgaag ccctacccat 300
aagggccatg aggacttgac gtcatcccca ccttcctccg gtttgtcacc ggcagtctcc 360
ctagagtgct cttgcgcagc aactaaggac aagggttgcg ctcgttgcgg gacttaaccc 420
aacatctcac gacacgagct gacgacagcc atgcagcacc tgtgtatcgg ttctctttcg 480
agcaccctca gatctctcca aggttccgac catgtcaagg gtaggtaagg tttttcgcgt 540
tgcatcgaat taatccacat catccaccgc ttgtgcgggt ccccgtcaat tcctttgagt 600
tttaatcttg cgaccgtact ccccaggcgg tcaacttcac gcgttagcta cgttactaag 660
gaaatgaatc cccaacaact agttgacatc gtttagggcg tggactacca gggtatctaa 720
tcctgtttgc tccccacgct ttcgtgcatg agcgtcagta ttggcccagg gggctgcctt 780
cgccatcggt attcctccac atctctacgc atttcactgc tacacgtgga attctactcc 840
cctctgccat actctagctt gccagtcacc aatgcagttc ccaggttgag cccggggatt 900
tcacatcggt cttaacaaac cgcctgcgca cgctttacgc ccagtaattc cgattaacgc 960
tcgcacccta cgtattaccg cggctgctgg cacgtagtta gccggtgctt attcttccgg 1020
taccgtcatc cccgaaggat attagccctc aggatttctt tccggacaaa agtgctttac 1080
aacccgaagg ccttcttcac acacgcggca ttgctggatc aggctttcgc ccattgtcca 1140
aaattcccca ctgctgcctc ccgtaggagt ctgggccgtg tctcagtccc agtgtggctg 1200
gtcgtcctct cagaccagct actgatcgtc gccttggtgg gcctttaccc caccaactag 1260
ctaatcagcc atcggccaac cctatagcgc gaggcccgaa ggtcccccgc tttcatccgt 1320
ggatcgtatg cggtattaat ccggctttcg ccgggctatc ccccactaca ggacatgttc 1380
cgatgtatta ctcacccgtt cgccactcgc caccaggtgc aagcacccgt gctgccgttc 1440
gacttgcatg tgtaaggcat gccgccagcg ttcaatctga gccatgatca aactct 1496
<210> 2
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<213> rDNA upstream primer of strain NSPE 2516S
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agagtttgat catggctcag 20
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<212> DNA
<213> rDNA downstream primer of strain NSPE 2516S
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acggttacct tgttacgact t 21
Claims (10)
1. An endophytic Burkholderia gladioli (Burkholderia gladioli) NSPE25, which is preserved in Guangdong province collection center for microorganism strains in 11-3 days 2020 and has the preservation number of GDMCC No: 61263, the preservation address is No. 59 building 5 of the No. 100 Dazhou prefecture of Guangzhou city, Guangdong province.
2. The use of the Burkholderia endophytic NSPE25 according to claim 1 for controlling fungal diseases in plants.
3. The use of the Burkholderia endophytic NSPE25 according to claim 1 for preparing a biological agent for controlling fungal plant diseases.
4. Use according to claim 2 or 3, wherein the plant fungal disease is a fruit tree fungal disease.
5. The use according to any one of claims 2 to 4, wherein the plant fungus is a Sphaerotheca pseudopekinensis, a Chitosa cinnabarina, a Fusarium aestivum or a Fusarium neoformans.
6. A biological agent comprising the Burkholderia gladioli NSPE25 according to claim 1.
7. The biological agent according to claim 6, wherein the biological agent comprises bacterial cells and/or bacterial liquid of Burkholderia gladioli NSPE 25.
8. A method for controlling fungal diseases of plants, characterized in that the biological agent according to claim 6 or 7 is used for controlling fungal diseases of plants.
9. The method of claim 8, wherein the plant is a fruit tree.
10. The method according to claim 8, wherein the plant fungus is a Pestalotiopsis, a Haematococcus cinnabarinus, a Fusarium aestivum or a Fusarium neoformans.
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Cited By (3)
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|---|---|---|---|---|
| CN114410544A (en) * | 2022-02-18 | 2022-04-29 | 西南林业大学 | Acinetobacter cinnamoylinum HZJK-2 and application thereof |
| CN115029280A (en) * | 2022-06-28 | 2022-09-09 | 甘肃省农业科学院蔬菜研究所 | Burkholderia gladioli for antagonizing cucumber fusarium wilt and application thereof |
| CN116218709A (en) * | 2022-12-16 | 2023-06-06 | 安徽农业大学 | Endophytic Burkholderia gladioli JS-59 and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105018371A (en) * | 2015-06-11 | 2015-11-04 | 江西师范大学 | Endophytic bacterium Burkholderia gladioli Fse32 of Dongxiang wild rice and application thereof |
| CN111925973A (en) * | 2020-09-21 | 2020-11-13 | 深圳职业技术学院 | Litchi endophytic Burkholderia gladioli and application thereof in preventing and treating litchi anthracnose and litchi frost blight |
| CN112011478A (en) * | 2020-07-31 | 2020-12-01 | 中国农业科学院农产品加工研究所 | Dendrobium nobile endogenous Burkholderia gladioli BL-HTie-5 and application thereof |
-
2021
- 2021-06-17 CN CN202110673727.6A patent/CN113337438B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105018371A (en) * | 2015-06-11 | 2015-11-04 | 江西师范大学 | Endophytic bacterium Burkholderia gladioli Fse32 of Dongxiang wild rice and application thereof |
| CN112011478A (en) * | 2020-07-31 | 2020-12-01 | 中国农业科学院农产品加工研究所 | Dendrobium nobile endogenous Burkholderia gladioli BL-HTie-5 and application thereof |
| CN111925973A (en) * | 2020-09-21 | 2020-11-13 | 深圳职业技术学院 | Litchi endophytic Burkholderia gladioli and application thereof in preventing and treating litchi anthracnose and litchi frost blight |
Non-Patent Citations (2)
| Title |
|---|
| HAZEM SE等: "In vitro antifungal activity of Burkholderia gladioli pv.agaricicola against some phytopathogenic fungi", 《INT.J.MOL.SCI》 * |
| 敖武等: "1株拮抗真菌的生防细菌Xj11的分离与鉴定", 《微生物学杂志》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114410544A (en) * | 2022-02-18 | 2022-04-29 | 西南林业大学 | Acinetobacter cinnamoylinum HZJK-2 and application thereof |
| CN114410544B (en) * | 2022-02-18 | 2023-06-23 | 西南林业大学 | Acinetobacter cinnarii HZJK-2 and application thereof |
| CN115029280A (en) * | 2022-06-28 | 2022-09-09 | 甘肃省农业科学院蔬菜研究所 | Burkholderia gladioli for antagonizing cucumber fusarium wilt and application thereof |
| CN115029280B (en) * | 2022-06-28 | 2023-08-18 | 甘肃省农业科学院蔬菜研究所 | Burkholderia gladioli for antagonizing cucumber fusarium wilt and application thereof |
| CN116218709A (en) * | 2022-12-16 | 2023-06-06 | 安徽农业大学 | Endophytic Burkholderia gladioli JS-59 and application thereof |
| CN116218709B (en) * | 2022-12-16 | 2023-12-15 | 安徽农业大学 | Endophytic Burkholderia gladioli JS-59 and application thereof |
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| CN113337438B (en) | 2021-12-24 |
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