CN113817636A - High-temperature-resistant nitrifying bacteria for reducing ammonia emission in sludge composting process and application thereof - Google Patents
High-temperature-resistant nitrifying bacteria for reducing ammonia emission in sludge composting process and application thereof Download PDFInfo
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/54—Nitrogen compounds
- B01D53/58—Ammonia
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
A high temperature resistant nitrifying bacterium for reducing ammonia emission in a sludge composting process and an application thereof belong to the technical field of environmental biology and relate to a high temperature resistant nitrifying bacterium. The invention aims to solve the problems that a large amount of ammonia gas is generated in the existing sludge composting process, and the release of foul smell limits the popularization and application of the sludge composting process. A high-temperature resistant nitrifying bacterium for reducing ammonia gas emission in a sludge composting process is Gordonia paraffinophilus N52, which is preserved in the common microorganism center of China Committee for culture Collection of microorganisms, the preservation address is No. 3 of No. 1 Hospital of Xilu of Shih of Chaozhou of Yangyang, the preservation date is 2021 year, 05 month and 13 days, and the preservation number is CGMCC No. 22519. A high-temperature resistant nitrifying bacterium for reducing the emission of ammonia gas in the sludge composting process is used for reducing the emission of ammonia gas in the sludge composting process.
Description
Technical Field
The invention belongs to the technical field of environmental biology, and relates to a high-temperature-resistant nitrifying bacterium.
Background
In recent years, the sewage treatment industry in China is rapidly developed, so that the sludge yield is rapidly increased. The sludge contains a large amount of organic pollutants, heavy metals, parasites, pathogenic microorganisms and the like, and meanwhile, malodorous gases are generated, and if the malodorous gases are not treated and discharged randomly, the malodorous gases can threaten water, air and soil environment greatly. Therefore, how to properly dispose of the sludge has become an important component in urban environmental protection.
The aerobic composting of the sludge is one of the main technologies of stabilizing the sludge, can convert organic pollutants into organic fertilizers, recycle nitrogen, phosphorus, potassium and other nutrient elements in the sludge, and reduce the toxicity and the content of pathogenic microorganisms. However, the release of a large amount of odor is a long-standing problem in the practical application of sludge compost, wherein ammonia gas is an odor with a large content. Generally, the release of ammonia gas in the stack mainly occurs in a high-temperature period, probably because the activity of nitrifying bacteria is inhibited, the conversion of ammonium ions to nitrate nitrogen is reduced, and the direct volatilization of ammonia gas is caused. The release of ammonia not only reduces the fertilizer efficiency of the compost product, but also produces a foul smell. Therefore, the release of ammonia gas in the sludge composting process can limit the popularization and application of the process.
Disclosure of Invention
The invention aims to solve the problems that a large amount of ammonia gas is generated in the existing sludge composting process, and the release of foul smell limits the popularization and application of the sludge composting process, and provides high-temperature-resistant nitrobacteria for reducing the emission of ammonia gas in the sludge composting process and application thereof.
A high-temperature resistant nitrifying bacterium for reducing ammonia gas emission in a sludge composting process is Gordonia paraffinophilus N52, which is preserved in the common microorganism center of China Committee for culture Collection of microorganisms, the preservation address is No. 3 of No. 1 Hospital of Xilu of Shih of Chaozhou of Yangyang, the preservation date is 2021 year, 05 month and 13 days, and the preservation number is CGMCC No. 22519.
Further, the highest tolerance temperature of the high-temperature resistant nitrifying bacteria for reducing the emission of ammonia gas in the sludge composting process is 60 ℃.
A high-temperature resistant nitrifying bacterium for reducing the emission of ammonia gas in the sludge composting process is used for reducing the emission of ammonia gas in the sludge composting process.
The invention relates to a high-temperature resistant nitrifying bacterium for reducing ammonia gas emission in a sludge composting process, which is Gordonia paraffiniformivorans (Gordonia paraffinivorans) N52 and has the following properties:
the separated bacterial strain N52 has orange colony, diameter of 2mm, no transparency, irregular shape and dry surface; the strain N52 is gram-positive bacteria, and an individual is in a short rod shape, the length of the strain is 1.25-1.31 mu m, and the width of the strain is 0.36-0.39 mu m. The physiological and biochemical characteristic experiments show that the catalase test, the glucose fermentation test, the methyl red test (M.R), the litmus milk test, the nitrite test, the gelatin liquefaction test, the starch hydrolysis test and the indole test of the strain N52 are positive; the oxidase test, the acetomethylalcohol test (V.P), the ammonia production test, the denitrification test and the hydrogen sulfide production test all show negative results. The tolerance temperature of the strain N52 is 60 ℃, and the growth and the reproduction of the strain are inhibited when the culture temperature exceeds 60 ℃.
Genomic DNA of the strain N52 was extracted and amplified using 16SrDNA sequencing, and as a result, it was found that the strain had a similarity of 99% or more to Gordonia Paraffinivorans (NR _028832.1) of GenBank database, and thus the strain was determined to be Gordonia paraffinivorans (Gordonia paraffinivorans). The sequence was uploaded to the GenBank database to obtain accession number MW 423577.
The invention has the following beneficial effects:
(1) the Gordonia paraffinivorans N52 culture method provided by the invention is simple, short in culture period and better in high temperature resistance, and can grow and propagate at a high temperature of 60 ℃;
(2) gordonia paraffinivorans N52 inoculated to the sludge compost can accelerate the temperature rise of the compost and prolong the high-temperature period, which is beneficial to killing the insect eggs in the compost material;
(3) gordonia paraffinivorans N52 can be nitrified under high temperature condition to convert ammonium ions into nitrate nitrogen;
(4) the Gordonia paraffinivorans N52 added into the sludge compost can reduce the emission of ammonia gas and simultaneously improve NO in the compost3 -The content of N reduces the loss of nitrogen in the compost product.
(5) By using Gordonia paraffinivorans N52 provided by the invention in the composting process, the highest temperature of the compost can reach 60.3 ℃, and the high temperature period in the composting process can be prolonged by 3 days;
(6) the Gordonia paraffinivorans N52 can obviously reduce the emission of ammonia gas by 27.63 percent in the composting process;
(7) the Gordonia paraffinivorans N52 provided by the invention is used in the composting process, NO3 -The N content can be increased by 16.75%.
In conclusion, the Gordonia paraffinivorans N52 provided by the invention can reduce the emission of ammonia gas and reduce the pollution of sludge compost to the environment when being applied to the sludge compost.
Drawings
FIG. 1 is a photograph of Gordonia paraffinivorans N52 colony;
FIG. 2 is a gram-stained photograph of Gordonia paraffinivorans N52 strain;
FIG. 3 is a scanning electron micrograph of Gordonia paraffinivorans N52 strain;
FIG. 4 is a graph showing the results of a phylogenetic tree of Gordonia paraffinivorans N52;
FIG. 5 is a diagram showing the detection results of the temperature of the compost in the sludge composting process in the experimental group and the control group;
FIG. 6 is a graph showing the results of measuring the instantaneous discharge of ammonia gas during sludge composting in the experimental group and the control group;
FIG. 7 is a graph showing the results of measuring the cumulative discharge of ammonia gas during the sludge composting process in the experimental group and the control group;
FIG. 8 shows NO in sludge composting process in experimental and control groups3 --N content test result graph.
Detailed Description
The following examples further illustrate the present invention but are not to be construed as limiting the invention. Modifications and substitutions to methods, procedures, or conditions of the invention may be made without departing from the spirit of the invention.
The first embodiment is as follows: the high-temperature resistant nitrobacteria for reducing ammonia gas emission in the sludge composting process is Gordonia paraffinophila N52 preserved in the common microorganism center of China Committee for culture Collection of microorganisms, the preservation address is No. 3 of Xilu No. 1 of Beijing Korean district, the preservation date is 2021 year 05 month 13 days, and the preservation number is CGMCC No. 22519.
The second embodiment is as follows: the present embodiment differs from the present embodiment in that: the highest tolerance temperature of the high-temperature resistant nitrifying bacteria for reducing the emission of ammonia gas in the sludge composting process is 60 ℃. Other steps are the same as in the first embodiment.
The third concrete implementation mode: the embodiment is that the high-temperature resistant nitrifying bacteria for reducing the emission of ammonia gas in the sludge composting process are used for reducing the emission of ammonia gas in the sludge composting process.
A high-temperature resistant nitrifying bacterium for reducing ammonia emission in a sludge composting process is Gordonia paraffinivorans N52, and the strain can promote conversion of ammonium ions in a compost to nitrate nitrogen, reduce emission of ammonia, reduce emission of odor in the compost, and increase nitrate Nitrogen (NO) of compost products3 --N) content, avoiding the loss of nitrogen in the compost product.
The following examples were used to demonstrate the beneficial effects of the present invention:
example 1:
the separation and identification of Gordonia paraffinivorans N52 specifically comprise the following steps:
firstly, taking 1g of a sludge compost high-temperature period (the temperature of a compost exceeds 55 ℃) sample, putting the sample into 100mL of sterile saline with the mass fraction of 0.9%, culturing for 4h under the conditions of 50 ℃ and 150r/min, then taking 10mL of the sample, inoculating the sample into 250mL of nitrifying liquid culture medium, and continuously culturing for 7d under the same conditions to enrich the strain;
② after 7 days of culture, taking enrichment culture solution to dilute in sequence, respectively taking 10-5、10-6、10-7200 mu L of diluent is put into a nitrifying solid culture medium, the constant temperature culture is carried out for 48h at 50 ℃, and after the visible colonies appear, the single colonies are picked and further purified;
and thirdly, selecting the purified single colony, inoculating the single colony in a nitrifying liquid culture medium, detecting the conversion capacity of the single colony to ammonium ions and the generation capacity of nitrate nitrogen, and selecting the strain with the strongest capacity, namely N52.
In the step 1, the sludge composting high-temperature period sample is from composting materials of Wenchang sewage treatment plant in Harbin city, Heilongjiang province.
The preparation method of the nitrifying liquid culture medium comprises the following steps: (NH)4)SO40.47g, sodium succinate 5.62g, K2HPO4 0.125g，MgSO4 0.125g，NaCl 0.125g，MnSO4·4H2O 0.0025g，FeSO4·7H20.0025g of O and 1000ml of distilled water, the pH value is 7.2-7.4, and sterilization is carried out for 20min at 121 ℃.
The preparation method of the nitrifying solid culture medium comprises the following steps: (NH)4)SO40.47g, sodium succinate 5.62g, K2HPO4 0.125g，MgSO4 0.125g，NaCl 0.125g，MnSO4·4H2O 0.0025g，FeSO4·7H20.0025g of O, 20g of agar and 1000ml of distilled water, wherein the pH value is 7.2-7.4, and the sterilization is carried out for 20min at 121 ℃.
The isolated strain N52, whose colony was orange, about 2mm in diameter, opaque, irregular, surface-dried (as shown in FIG. 1); the bacterial strain is gram-positive bacteria, and an individual presents a short rod shape, the length is 1.25-1.31 mu m, and the width is 0.36-0.39 mu m (as shown in figures 2 and 3).
The catalase test, the glucose fermentation test, the methyl red test (M.R), the litmus milk test, the nitrite test, the gelatin liquefaction test, the starch hydrolysis test and the indole test of the strain N52 are all positive; the oxidase test, the acetomethylalcohol test (V.P), the ammonia production test, the denitrification test and the hydrogen sulfide production test all show negative results. The tolerance temperature of the strain N52 is 60 ℃, and the growth and the reproduction of the strain are inhibited when the culture temperature exceeds 60 ℃.
Genomic DNA of strain N52 was extracted and amplified using 16SrDNA sequencing technology and strain comparisons were performed in the National Center for Biotechnology Information, NCBI database. The detailed information is as follows:
primer 27F: 5 '-AGTTTGATCMTGGCTCAG-3'
Primer 1492R: 5'-GGTTACCTTGTTACGACTT-3'
The sequence of the 16SrDNA obtained by amplification is shown in Table 1, and the total length is 1471 bp. The sequencing results of the strains were compared in sequence, and the phylogenetic tree results of the constructed strains are shown in FIG. 4. As a result, it was found that the strain N52 had a similarity of 99% or more to Gordonia Paraffinivorans (NR _028832.1) in the GenBank database, and the strain N52 was judged to be Gordonia paraffinivorans (Gordonia paraffinivorans) based on the results of physiological and biochemical characteristics and molecular biological identification. The identified Gordonia Paraffinivorans N52 is preserved in China general microbiological culture Collection center (CGMCC) at 05-13.2021, with the strain preservation number of CGMCC No. 22519.
Experimental group composting (inoculum):
1) selecting Gordonia Paraffinivorans N52 growing on nitrifying solid medium, inoculating in sterilized nitrifying liquid culture medium, culturing at 50 deg.C and 150r/min for 48 hr, inoculating in newly sterilized nitrifying liquid culture medium with 1% inoculum size, and performing amplification culture for 48 hr to obtain culture medium with bacteria content of more than 1 × 108cuf/ml of bacterial suspension.
2) Preparing compost raw materials, fresh sludge with the humidity of 73% and rice hulls with the water content of 11% after crushing, wherein the mass ratio of the fresh sludge to the crushed rice hulls is 3: 1 (sludge: rice hull) are mixed uniformly and then aerobic composting is carried out, and air is ventilated from the bottom of a compost body by using an air pump, wherein the ventilation rate is 0.3L/min.
Preparing bacterial suspension (strain N52) cultured for 48h in the step 1, centrifuging for 10min at 5000rmp, discarding the upper liquid culture medium, and resuspending with small amount of sterile water to obtain liquid microbial inoculum (with bacterial content of 1 × 10) added with heap8CFU/ml). Gordonia paraffinivorans N52 was inoculated into the compost at 0d at a inoculum size of 3% for aerobic composting for 25 d.
This example is an experimental group.
Control compost (no added bacteria):
1) fresh sludge of Longjiang environmental protection group Limited company with the humidity of 73% and crushed rice hulls with the water content of 11% are mixed according to the mass ratio of 3: 1, uniformly mixing, adding sterile water with the same amount as the bacterial liquid to ensure that the water content of the raw materials of the compost is the same, and carrying out forced ventilation on the bottom of the compost, wherein the ventilation rate is 0.3L/min, and the composting time is 25 d.
This example is a control group.
The temperature of the heap of the experimental and control groups during composting was measured by a thermometer inside the heap and recorded every 24h, and the temperature change in the heap is shown in figure 4.
The ammonia gas volatilization amount and the accumulated volatilization amount were measured by the boric acid absorption method during the composting process in the experimental group and the control group, as shown in fig. 5. Using boric acid solution with the mass fraction of 2% as absorption liquid, dripping methyl red-bromocresol green into the absorption liquid as a mixed indicator, wherein the color is purple red, performing closed absorption, changing the absorption liquid every 24h, and titrating the absorption liquid with standard hydrochloric acid solution to the end point.
In the composting process of an experimental group and a control group, a fresh compost sample is leached for 1h by 2mol/L KCl solution according to the proportion of 1:10(w/v), the mixed solution is centrifuged, the supernatant is filtered by a 0.45 mu m fiber resin filter membrane, the filtrate is collected, and then the NO is measured by adopting an ultraviolet absorption method3 -Content of-N, NO in compost3 -the-N content is shown in FIG. 6.
The experimental results show that:
1) after Gordonia paraffinivorans N52 was inoculated into the experimental group, the temperature of the pack rapidly increased to 60.3 ℃ at the highest temperature, and 56.0 ℃ at the highest temperature in the control group; meanwhile, the time for maintaining the high-temperature period in the composting process of the experimental group is 6d, while the time for maintaining the high-temperature period in the control group is 3d, and the time for maintaining the high-temperature period in the experimental group is prolonged by 3d compared with that in the control group.
2) The test group to which Gordonia paraffinivorans N52 was added significantly reduced the ammonia emission during composting by 27.63% compared to the control group.
3) NO of experimental group after compost is finished3 -NO in the test group at 25 days with an N content of 817.99mg/kg and a control group of 680.98mg/kg3 --N content ratio of NO in control group3 -The N content is increased by 16.75%.
Therefore, the Gordonia paraffinivorans N52 can prolong the high-temperature period of sludge composting, reduce the discharge amount of ammonia gas in the composting process and increase NO in the compost body3 --N content.
<110> Harbin Industrial university
<120> high-temperature-resistant nitrifying bacteria for reducing ammonia gas emission in sludge composting process and application thereof
<160> 3
<210> 1
<211> 1471
<212> DNA
<213> Gordonia paraffinivorans (Gordonia paraffinivorans) N52.
gatcatggct caggacgaac gctggcggcg tgcttaacac atgcaagtcg aacggaaagg 60
cccagcttgc tgggtactcg agtggcgaac gggtgagtaa cacgtgggtg atctgccctg 120
gactctggga taagcctggg aaactgggtc taataccgga tattcacttt ctctcgcatg 180
ggggttggtg gaaagctttt gcggttcagg atgggcccgc ggcctatcag cttgttggtg 240
gggtaatggc ctaccaaggc gacgacgggt agccgacctg agagggtgat cggccacact 300
gggactgaga cacggcccag actcctacgg gaggcagcag tggggaatat tgcacaatgg 360
gcgcaagcct gatgcagcga cgccgcgtga gggatgacgg ccttcgggtt gtaaacctct 420
ttcaccaggg acgaagcgtg agtgacggta cctggagaag aagcaccggc caactacgtg 480
ccagcagccg cggtaatacg tagggtgcga gcgttgtccg gaattactgg gcgtaaagag 540
ctcgtaggcg gtttgtcgcg tcgtctgtga aattctgcaa ctcaattgta ggcgtgcagg 600
cgatacgggc agacttgagt actacagggg agactggaat tcctggtgta gcggtgaaat 660
gcgcagatat caggaggaac accggtggcg aaggcgggtc tctgggtagt aactgacgct 720
gaggagcgaa agcgtgggga gcgaacagga ttagataccc tggtagtcca cgccgtaaac 780
ggtgggtact aggtgtggga ctcttttcac gggttccgtg ccgtagctaa cgcattaagt 840
accccgcctg gggagtacgg ccgcaaggct aaaactcaaa ggaattgacg ggggcccgca 900
caagcggcgg agcatgtgga ttaattcgat gcaacgcgaa gaaccttacc tgggtttgac 960
atctaccgga ccgctccaga gatggggctt cccttgtggc tggtagacag gtggtgcatg 1020
gctgtcgtca gctcgtgtcg tgagatgttg ggttaagtcc cgcaacgagc gcaacccttg 1080
tcctgtattg ccagcgcgta atggcgggga cttgcaggag actgccgggg tcaactcgga 1140
ggaaggtggg gatgacgtca agtcatcatg ccccttatgt ccagggcttc acacatgcta 1200
caatggctgg tacagagggc tgcgataccg tgaggtggag cgaatccctt aaagccagtc 1260
tcagttcgga ttggggtctg caactcgacc ccatgaagtc ggagtcgcta gtaatcgcag 1320
atcagcaacg ctgcggtgaa tacgttcccg ggccttgtac acaccgcccg tcacgtcatg 1380
aaagtcggta acacccgaag ccggtggcct aaccccttgt gggagggagc tgtcgaaggt 1440
gggatcggcg attgggacga agtcgtaaca a 1471
<210>2
<211>18
<212> DNA
<213> Artificial sequence
<220>
<223>27F。
<210>3
<211>19
<212> DNA
<213> Artificial sequence
<220>
<223>1492R。
GGTTACCTTGTTACGACTT 19
Claims (3)
1. The high temperature resistant nitrifying bacteria capable of reducing ammonia gas emission in the sludge composting process is characterized in that the nitrifying bacteria are Gordonia paraffinophila N52, are preserved in the common microorganism center of China Committee for culture Collection of microorganisms, are preserved in No. 3 of Xilu No. 1 of Beijing Kogyo-sunward area, have the preservation date of 2021 year, 05 month and 13 days, and have the preservation number of CGMCC No. 22519.
2. The thermophilic nitrifying bacterium for reducing the emission of ammonia gas in a sludge composting process as claimed in claim 1 wherein the thermophilic nitrifying bacterium for reducing the emission of ammonia gas in a sludge composting process has a maximum tolerance temperature of 60 ℃.
3. The use of the thermophilic nitrifying bacterium for reducing the emission of ammonia gas in a sludge composting process as claimed in claim 1, wherein a thermophilic nitrifying bacterium for reducing the emission of ammonia gas in a sludge composting process is used for reducing the emission of ammonia gas in a sludge composting process.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115286441A (en) * | 2022-08-04 | 2022-11-04 | 黑龙江省科学院微生物研究所 | Method for reducing release of nitrogen-containing gas in sludge composting process |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1699547A (en) * | 2005-05-24 | 2005-11-23 | 南开大学 | Gordona terrae C-6 and its desulfurization effect |
| KR100666723B1 (en) * | 2006-01-19 | 2007-01-09 | 정욱진 | A sulfer-based autotrophic denitrifying microorganism Gordonia sp. CR which could remove nitrate nitrogen of high concentration |
| JP2018188550A (en) * | 2017-05-02 | 2018-11-29 | シーバイエス株式会社 | Antibacterial detergent composition for dishwasher |
| CN109402016A (en) * | 2018-09-21 | 2019-03-01 | 江苏宜裕环保科技有限公司 | For the complex micro organism fungicide of chemical wastewater treatment and its screening and preparation method |
| CN110526421A (en) * | 2019-09-10 | 2019-12-03 | 中交铁道设计研究总院有限公司 | A method of heavy metal ions in sewage is removed using Gordonia bronchialis is hooked |
| CN111778187A (en) * | 2020-07-07 | 2020-10-16 | 内蒙古恒盛环保科技工程有限公司 | Microbial repairing microbial inoculum and preparation method thereof |
| CN113512515A (en) * | 2021-03-23 | 2021-10-19 | 常州大学 | Ammonia oxidation complex microbial inoculant and preparation method and application thereof |
| CN115286441A (en) * | 2022-08-04 | 2022-11-04 | 黑龙江省科学院微生物研究所 | Method for reducing release of nitrogen-containing gas in sludge composting process |
-
2021
- 2021-09-22 CN CN202111106370.XA patent/CN113817636B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1699547A (en) * | 2005-05-24 | 2005-11-23 | 南开大学 | Gordona terrae C-6 and its desulfurization effect |
| KR100666723B1 (en) * | 2006-01-19 | 2007-01-09 | 정욱진 | A sulfer-based autotrophic denitrifying microorganism Gordonia sp. CR which could remove nitrate nitrogen of high concentration |
| JP2018188550A (en) * | 2017-05-02 | 2018-11-29 | シーバイエス株式会社 | Antibacterial detergent composition for dishwasher |
| CN109402016A (en) * | 2018-09-21 | 2019-03-01 | 江苏宜裕环保科技有限公司 | For the complex micro organism fungicide of chemical wastewater treatment and its screening and preparation method |
| CN110526421A (en) * | 2019-09-10 | 2019-12-03 | 中交铁道设计研究总院有限公司 | A method of heavy metal ions in sewage is removed using Gordonia bronchialis is hooked |
| CN111778187A (en) * | 2020-07-07 | 2020-10-16 | 内蒙古恒盛环保科技工程有限公司 | Microbial repairing microbial inoculum and preparation method thereof |
| CN113512515A (en) * | 2021-03-23 | 2021-10-19 | 常州大学 | Ammonia oxidation complex microbial inoculant and preparation method and application thereof |
| CN115286441A (en) * | 2022-08-04 | 2022-11-04 | 黑龙江省科学院微生物研究所 | Method for reducing release of nitrogen-containing gas in sludge composting process |
Non-Patent Citations (3)
| Title |
|---|
| ZHAO Y 等: "Gordonia sp.(in:high G+C Gram-positive bacteria) strain N52 16S ribosomal RNA gene,partial sequence" * |
| ZHAO 等: "Isolation and application of a thermotolerant nitrifying bacterium Gordonia paraffinivorans N52 in sewage sludge composting for reducing nitrogenloss" * |
| 曹林 等: "活性污泥中异养硝化-好氧反硝化菌的分离及其脱氮性能" * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115286441A (en) * | 2022-08-04 | 2022-11-04 | 黑龙江省科学院微生物研究所 | Method for reducing release of nitrogen-containing gas in sludge composting process |
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