CN113512515A - Ammonia oxidation complex microbial inoculant and preparation method and application thereof - Google Patents
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Abstract
The invention belongs to the technical field of microbial agents, and particularly relates to an ammonia oxidation complex microbial agent as well as a preparation method and application thereof. The novel ammonia oxidation complex microbial inoculum CCZU C6 is obtained by compounding three strains of efficient ammonia oxidation bacteria including paracoccus denitrificans TD-10, gordonia bacteria TD-46 and alcaligenes faecalis TD-94. The composite microbial inoculum has the advantages of high denitrification rate, strong environment resistance and the like, and after the composite microbial inoculum is added for 48 hours, the ammonia nitrogen is reduced to 0.9mg/L from the initial 155.8mg/L, and the ammonia nitrogen degradation rate reaches 77.4 mg/L.d‑1The ammonia nitrogen removal rate reaches 99 percent, and the method can be widely applied to the treatment of high ammonia nitrogen sewage such as coal chemical industry sewage, fermentation sewage, chemical industry and pharmaceutical production sewage and the like.
Description
Technical Field
The invention belongs to the technical field of microbial agents, and particularly relates to an ammonia oxidation complex microbial agent, and a preparation method and application thereof.
Background
In recent years, the coal hydrogen production technology is widely applied, the situation of energy shortage in China is relieved, and meanwhile, the generation of a large amount of coal hydrogen production wastewater limits the further development of the coal hydrogen production industry. The coal-to-hydrogen wastewater has the characteristics of large discharge amount, high ammonia nitrogen concentration, complex components, difficult treatment and the like. A large amount of ammonia nitrogen is discharged to cause river pollution, so that lake water can not be drunk. Moreover, ammonia nitrogen pollution can also cause red tide phenomenon in the sea area nearby China. Therefore, the rapid and economic degradation of high ammonia nitrogen wastewater is an urgent need of numerous petrochemical industries. The physicochemical method for treating the high ammonia nitrogen wastewater has simple operation and stable effect, but the technology has higher cost and more serious secondary pollution; the traditional biological denitrification technology widely used at present has the characteristics of slow reaction, low denitrification rate and large oxygen demand due to the long growth period of autotrophic ammonia oxidizing bacteria, and is often difficult to effectively treat the coal hydrogen production high ammonia nitrogen wastewater.
With the continuous development of the technology, the heterotrophic ammonia oxidation bacteria have unique advantages, so that the nitrification process is simpler and faster, the environmental interference resistance is superior, and the ammonia nitrogen removal rate is greatly improved. However, the coal hydrogen production wastewater has complex components, the ammonia nitrogen concentration and the COD concentration can respectively reach more than 800mg/L and 1000mg/L, and simultaneously has higher calcium and magnesium ion concentration, so that the coal hydrogen production wastewater has high component fluctuation. When a sewage treatment plant carries out denitrification and purification treatment on coal-to-hydrogen wastewater, the active sludge in a nitrification tank and a biochemical treatment tank is unstable due to large fluctuation of the components of the coal-to-hydrogen wastewater, and the treatment rate is reduced after the operation for a period of time.
Disclosure of Invention
In order to solve the technical problems, the invention provides an ammonia oxidation complex microbial inoculant, and a preparation method and application thereof. The composite microbial inoculum has the characteristics of various types, reasonable collocation, strong functionality and strong anti-environmental interference capability, can not damage the water area and the surrounding environment, is convenient to use, can be adsorbed in activated sludge to stably survive in actual sewage treatment, becomes a dominant strain, has stable ammonia nitrogen degradation effect, and reduces the investment cost.
The invention is realized by adopting the following technical scheme:
a novel high-efficiency ammonia oxidation complex microbial inoculum comprises three high-efficiency ammonia oxidation bacteria, one is denitrificated Paracoccus (Paracoccus sp.), the taxonomy of the strain is named Paracoccus sp.TD-10, and the preservation number is CGMCC NO. 19059; one strain is Gordonia sp (Gordonia sp.), the taxonomic name of the strain is Gordonia sp.TD-46, and the preservation number is CGMCC NO. 19057; one strain belongs to the Alcaligenes faecalis sp.TD-94, the collection number is CGMCC NO.19058, and the three strains are preserved in the general microbiological center of China Committee for culture Collection of microorganisms (CGMCC for short, and the address is No. 3 of West Lu 1 of the morning area of Beijing city) within 12 months and 2 days of 2019.
A preparation method of a high-efficiency ammonia oxidation complex microbial inoculum comprises the following specific steps:
(1) preliminary construction of Complex microbial inoculum
Transferring the five screened high-efficiency ammonia oxidizing strains into seed culture media respectively. Activation was carried out at 160rpm for 24h at 28 ℃. Compounding every two strains in a logarithmic growth phase of each strain, wherein the compounding ratio is 1: 1, inoculating 6%, and culturing in optimized basic culture medium. And measuring the ammonia nitrogen content and the nitrite nitrogen content of each group of screening culture medium after 5 days, analyzing the ammonia nitrogen degradation rate of various combinations, and selecting the compound combination with the minimum antagonism.
Seed culture medium: 10g of peptone, 5g of yeast powder, 10g of NaCl, 1L of distilled water and 7.0-7.4 of pH; optimizing a basic culture medium: NH (NH)4Cl 0.382g，CH3COONa 2g，MgSO4·7H2O 0.05g，K2HPO4 0.2g，NaCl 0.12g，MnSO4·4H2O 0.01g，FeSO4·7H2O0.01 g, distilled water 1L, pH 7.0-7.4.
(2) Compound microbial inoculum is constructed by compounding table
The method specifically comprises the following steps: activating three high-efficiency ammonia oxidizing bacteria in a seed culture medium for 24 hours, designing a compound proportion table by taking the inoculation amount of 1%, 2% and 4% of each strain as a variable, preparing a compound microbial inoculum according to the compound proportion table, transferring the compound microbial inoculum into an optimized basal culture medium, performing shake flask culture at 28 ℃, 160rpm for 2 days, measuring the ammonia nitrogen content in the culture medium, selecting the combination with the lowest ammonia nitrogen residual content, and determining the optimal compound.
Seed culture medium: 10g of peptone, 5g of yeast powder, 10g of NaCl, 1L of distilled water and 7.0-7.4 of pH; optimizing a basic culture medium: NH (NH)4Cl 0.382g，CH3COONa 2g，MgSO4·7H2O 0.05g， K2HPO4 0.2g，NaCl 0.12g，MnSO4·4H2O 0.01g，FeSO4·7H2O0.01 g, distilled water 1L, pH 7.0-7.4.
The ammonia oxidation complex microbial inoculum of the invention is prepared by respectively carrying out enrichment culture on three high-efficiency ammonia oxidation bacteria by seed culture media to obtain seed solutions of three strains according to the ratio of TD-94: TD-10: TD-46 ═ 4: 1: 4 (volume ratio) to obtain the composite microbial inoculum CCZU C6, and the preparation method has simple operation and easy control of the process.
(3) By optimizing the degradation conditions of the composite bacterial agent such as carbon source, nitrogen source, C/N ratio, initial pH, temperature, rotating speed and the like, the novel efficient ammonia oxidation composite bacterial agent CCZU C6 is obtained.
The degradation conditions are as follows: selecting glucose, sucrose, sodium acetate, sodium succinate, sodium citrate and potassium sodium tartrate as alternative carbon sources; selecting 100-500mg/L ammonium chloride as a nitrogen source; the C/N ratio ranges from 2 to 18; the initial pH value is 5.2-9.2; the temperature range is 24-34 ℃; the rotation speed range is between 140 and 220 rpm.
Further, the optimal degradation conditions are: selecting sodium acetate as a unique carbon source, NH4 +Concentration of-N300 mg/L, C/N ratio 8, initialThe initial pH was 7.2, the cultivation temperature was 28 ℃ and the rotational speed of the shaker was 200 rpm.
The novel efficient ammonia oxidation complex microbial inoculum is used in coal hydrogen production high ammonia nitrogen wastewater.
The specific application method comprises the following steps: laboratory shake flask pilot test is carried out on wastewater of a simulated factory, and then sludge and wastewater in a famous petrochemical A/O process pool are collected to carry out factory pilot test.
The compound microbial inoculum of the invention has the following advantages:
firstly, the preparation method of the complex microbial inoculum is simple to operate, the process is easy to control, and the water area and the surrounding environment cannot be damaged;
the three strains of the composite microbial inoculum have a synergistic effect, the bacterial community is richer when the composite microbial inoculum is added into wastewater, the species diversity is improved, the three strains become dominant strains, and the species abundance is continuously improved, so that the biological denitrification capability of the composite microbial inoculum is enhanced, and the ammonia nitrogen in the wastewater is rapidly degraded.
The composite microbial inoculum has efficient denitrification performance, can stably survive in activated sludge for a long time, biologically strengthens the activated sludge, and further improves the rate of treating coal hydrogen production wastewater.
The composite microbial inoculum has the advantages of high denitrification rate, strong environment resistance and the like, and can be widely applied to the treatment of high ammonia nitrogen sewage such as coal chemical industry sewage, fermentation sewage, chemical industry and pharmaceutical production sewage and the like.
The invention has the beneficial effects that: three high-efficiency ammonia oxidizing bacteria are compounded to obtain a novel high-efficiency ammonia oxidizing composite bacterial agent CCZU C6, after the composite bacterial agent is added for 48 hours, the ammonia nitrogen is reduced to 0.9mg/L from the initial 155.8mg/L, and the degradation rate of the ammonia nitrogen reaches 77.4 mg/L.d-1The ammonia nitrogen removal rate reaches 99 percent. The composite microbial inoculum has the advantages of high denitrification rate, strong environment resistance and the like, can be widely applied to the treatment of high ammonia nitrogen sewage such as coal chemical industry sewage, fermentation sewage, chemical industry and pharmaceutical production sewage and the like, and simultaneously has simple operation and easy control of the process, thereby having good industrial application prospect.
The technical solutions of the present invention are further described in detail with reference to the drawings and specific examples, which are provided for illustrative purposes only and do not limit the present invention in any way.
Drawings
FIG. 1 shows the ammonia nitrogen content and nitrite nitrogen content of single strain and each compound combination;
FIG. 2 shows the effect of different carbon sources on ammonia nitrogen degradation of the composite microbial inoculum CCZU C6;
FIG. 3 shows the effect of different nitrogen source concentrations on ammonia nitrogen degradation of the complex microbial inoculum CCZU C6;
FIG. 4 shows the influence of C/N ratio on ammonia nitrogen degradation of a complex microbial inoculum CCZU C6;
FIG. 5 shows the influence of the initial pH on ammonia nitrogen degradation of the complex microbial inoculum CCZU C6;
FIG. 6 shows the effect of the culture temperature on the degradation of ammonia nitrogen by the composite microbial inoculum CCZU C6;
FIG. 7 shows the effect of dissolved oxygen on ammonia nitrogen degradation of a composite microbial inoculum CCZU C6;
FIG. 8 shows the results of shake flask experiments;
FIG. 9 shows the results of a famous petrochemical pilot plant.
Detailed Description
The reagents adopted by the invention are all commercially available analytical purifications or more, and the strains used in the test are laboratory preservation strains of Changzhou university. The method for measuring ammonia nitrogen and nitrite nitrogen comprises the following steps: adopts a nano reagent spectrophotometry (HJ 35-2009) for measuring ammonia nitrogen in water quality of national environmental protection standard of the people's republic of China and a spectrophotometry (GB/T7493-1987) for measuring nitrite nitrogen in water quality.
Example 1
(1) Preliminary construction of Complex microbial inoculum
Transferring the five screened high-efficiency ammonia oxidizing strains into seed culture media respectively. Activation was carried out at 160rpm for 24h at 28 ℃. Compounding every two strains in a logarithmic growth phase of each strain, wherein the compounding ratio is 1: 1, the inoculation amount is 6 percent, and the mixture is transferred into an optimized basic culture medium (the initial ammonia nitrogen content is 100mg/L) for culture. After 5 days, the ammonia nitrogen content and the nitrite nitrogen content of each group of screening culture medium are measured, the ammonia nitrogen degradation rate of various combinations is analyzed, and the compound combination with the minimum antagonism is selected, and the result is shown in figure 1.
As can be seen from figure 1, the effect of the compound combination TD-10+ TD-94 and TD-46+ TD-94 is better. As can be seen from FIG. 1 (b), TD-10 and TD-46 showed low production of nitrite nitrogen, while too high a production of nitrite nitrogen inhibited the growth of ammonia oxidizing bacteria and the degradation rate of ammonia nitrogen. Therefore, the two strains have better compounding effect with TD-94. TD-10, TD-46 and TD-94 bacteria are selected to explore the optimal compound ratio of the three bacteria.
(2) Compound microbial inoculum is constructed by compounding table
Three ammonia oxidizing strains are respectively inoculated into a seed culture medium. Activation was carried out at 160rpm for 24h at 28 ℃. The bacterial strains are compounded during the logarithmic growth phase of each bacterial strain, a compounding ratio table (shown in table 1) is designed by taking the inoculation amount of 1%, 2% and 4% of each bacterial strain as a variable, a compound microbial inoculum is prepared according to the compounding ratio table and is transferred into an optimized basal culture medium (the initial ammonia nitrogen content is 100mg/L), the ammonia nitrogen content in the culture medium is measured after shaking culture at 28 ℃ and 160rpm for two days, the combination with the lowest ammonia nitrogen residual content is selected, and the optimal compounding is determined.
Seed culture medium: 10g of peptone, 5g of yeast powder, 10g of NaCl, 1L of distilled water and 7.0-7.4 of pH; optimizing a basic culture medium: NH (NH)4Cl 0.382g，CH3COONa 2g，MgSO4·7H2O 0.05g， K2HPO4 0.2g，NaCl 0.12g，MnSO4·4H2O 0.01g，FeSO4·7H2O0.01 g, distilled water 1L, pH 7.0-7.4.
TABLE 1 compounding ratio table
The results in Table 1 show that the sixth group of compositions has a composition ratio of TD-94: TD-10: TD-46 ═ 4: 1: 4, the ammonia nitrogen residual content is lowest, and the ammonia nitrogen degradation rate is highest, so that a novel efficient ammonia oxidation complex bacterial agent is constructed and named as complex bacterial agent CCZU C6. After two days of culture, the denitrification rate of the composite microbial inoculum CCZU C6 reaches 41.74mg/L d-1The ammonia nitrogen removal rate of 48 hours can reach 83.48 percent。
Example 2
The influence of different carbon sources on the degradation of ammonia nitrogen by the composite microbial inoculum CCZU C6 is examined, glucose, sucrose, sodium acetate, sodium succinate, sodium citrate and potassium sodium tartrate are selected as alternative carbon sources, each group of experiments are contrasted for 3 times, and the specific experiment operation is as follows:
adding different carbon sources into an optimized basal culture medium, activating the compound microbial inoculum CCZU C6 in a seed culture medium for 24h, transferring the activated compound microbial inoculum CCZU C6 into the optimized basal culture medium in the logarithmic growth phase of the activated compound microbial inoculum by using a liquid transfer gun, performing shake-flask culture at 28 ℃, 160rpm for 5 days, and detecting NH in the culture medium every day4 +And NO2 -The results are shown in FIG. 2.
This example illustrates that the ammonia nitrogen degradation rate of the complex microbial inoculum CCZU C6 in a culture medium using an organic acid compound as a carbon source is significantly higher than that of other culture media, the nitrite nitrogen content of the culture medium using an organic acid compound as a carbon source corresponding to the complex microbial inoculum CCZU C6 is relatively high, and sodium acetate is finally selected as the optimal carbon source.
Example 3
And (3) investigating the influence of the composite microbial inoculum CCZU C6 on ammonia nitrogen degradation under different ammonia nitrogen concentrations. Ammonium chloride is selected as a unique carbon source, the nitrogen source concentration is respectively set to be 100mg/L, 150mg/L, 200mg/L, 250mg/L, 300mg/L, 350mg/L, 400mg/L, 450mg/L and 500mg/L, each group of experiments is subjected to 3 times of comparison, the specific experiment operation is shown in example 2, and the result is shown in figure 3.
This example illustrates that, in the culture medium with different nitrogen source concentrations, when the nitrogen source concentration in the degradation culture medium is gradually increased from 100mg/L to 300mg/L, the degradation rate of ammonia nitrogen is slowly increased, and the nitrite production rate is continuously increased. When the concentration of the nitrogen source in the degradation medium is continuously increased to 500mg/L, the degradation rate of ammonia nitrogen is gradually reduced, and the content of nitrite is continuously reduced. The continuous increase of the nitrogen source concentration can provide sufficient heterotrophy for the growth of microorganisms, and can improve the ammonia nitrogen degradation rate of the compound microbial inoculum; and the excessively high nitrogen source concentration limits the growth of the compound microbial inoculum CCZU C6, so that ammonia nitrogen cannot be degraded, and finally 300mg/L is selected as the optimal ammonia nitrogen concentration.
Example 4
And (3) investigating the influence of the C/N ratio on the degradation of ammonia nitrogen by the composite microbial inoculum CCZU C6. The optimal carbon source and ammonia nitrogen concentration are selected, the C/N ratio is set to be 2, 5, 8, 10, 14 and 18 respectively, 3 times of comparison is carried out on each group of experiments, specific experiment operation is shown in example 2, and the result is shown in figure 4.
This example illustrates that too low a carbon source concentration makes it difficult for the microorganism to continue growing, while too high a carbon source concentration makes the microorganism produce a large amount of harmful metabolites, while also increasing plant costs, due to essential factors for microbial growth in carbon source. Thus, the optimal C/N ratio is finally selected to be 8.
Example 5
And (3) investigating the influence of the initial pH value on the degradation of ammonia nitrogen by the composite microbial inoculum CCZU C6. Setting the initial pH value of the culture medium to be 5.2, 6.2, 7.2, 8.2 and 9.2, and making 3 controls for each group of experiments, wherein the specific experiment operations are as follows:
activating the complex microbial inoculum CCZU C6 in a seed culture medium for 24h, transferring the complex microbial inoculum to an optimized basic culture medium with optimal carbon source, nitrogen source concentration and C/N selection by using a liquid transfer gun in the logarithmic growth phase, setting the initial pH values of the optimized basic culture medium to be 5.2, 6.2, 7.2, 8.2 and 9.2, shaking the culture medium at 28 ℃ and 160rpm for 5 days, and detecting NH in the culture medium every day4 +And NO2 -The results are shown in FIG. 5.
This example shows that when the pH is too low, the ammonia nitrogen degradation effect of the complex microbial inoculum CCZU C6 is significantly inhibited, and is much greater than the inhibition effect caused by too high pH. When the pH is between 7.2 and 8.2, the denitrification rate of the complex microbial inoculum CCZU C6 is high, so the denitrification rate is optimal when the pH is selected to be 7.2.
Example 6
And (3) investigating the influence of the culture temperature on the ammonia nitrogen degradation of the composite microbial inoculum CCZU C6. The culture temperature of the culture medium was set at 24 ℃, 26 ℃, 28 ℃, 30 ℃, 32 ℃ and 34 ℃, 3 controls were made for each set of experiments, the specific experimental procedures are shown in example 5, and the results are shown in fig. 6.
This example shows that the inhibitory effect of low temperature on ammonia oxidizing bacteria is obvious, and the tolerance of the complex microbial inoculum CCZU C6 to temperature increase is better, so 28 ℃ is finally selected as the optimal culture temperature.
Example 7
And (3) investigating the influence of dissolved oxygen on ammonia nitrogen degradation of the composite microbial inoculum CCZU C6. The table rotation speed was set at 140rpm, 160rpm, 180rpm, 200rpm, 220rpm, and 3 controls were made for each set of experiments, the specific experiment operation is shown in example 5, and the results are shown in FIG. 7.
This example illustrates that too low or too high rotation speed will reduce the degradation rate of ammonia nitrogen, but the effect is less, when the rotation speed is 200rpm, the degradation rate of ammonia nitrogen is the highest, so 200rpm is finally selected as the optimum table rotation speed.
Example 8
The application of the novel high-efficiency ammonia oxidation complex microbial inoculum in the coal-to-hydrogen high ammonia nitrogen wastewater is as follows: the laboratory shake flask pilot test is carried out by simulating the wastewater of a factory, and the specific test operation is as follows:
the shaking flask pilot test is to collect sludge and wastewater from China petrochemical Ministry company so as to simulate the wastewater of a factory to carry out laboratory culture, so as to research the environmental adaptability of the composite microbial inoculum CCZU C6, thereby providing theoretical guidance for the amplified pilot test of the factory. Six different control group tests are set, 50mL of wastewater (initial ammonia nitrogen 191mg/L, COD1400 mg/L) is measured in each group and put in a 250mL conical flask, sludge is added, sodium acetate is added, C/N is set to be 8, the inoculum size is 10%, shaking culture is carried out at 28 ℃ and 160rpm, and the ammonia nitrogen and nitrite nitrogen content in the water is detected every day. Each set of experiments was controlled 3 times and the results are shown in figure 8.
The embodiment shows that when the composite microbial inoculum and other strains in the sludge grow together, the ammonia nitrogen degradation rate is more prominent, and the composite microbial inoculum CCZU C6 and other microorganisms in the wastewater can be symbiotic and rapidly become dominant strains, so that the denitrification reaction is performed more efficiently.
Example 9
The application of the novel high-efficiency ammonia oxidation complex microbial inoculum in the coal-to-hydrogen high ammonia nitrogen wastewater is II: collecting sludge and wastewater in a named petrochemical A/O process pool to perform factory pilot test, wherein the specific test operation is as follows:
20L of high ammonia nitrogen wastewater (COD 1321mg/L, ammonia nitrogen 155.8mg/L, alkalinity 821.11mg/L, pH 8.6) and 20L of activated sludge are collected from a famous petrochemical A/O process tank, and put into a device, an air pump is started to aerate and stir for two hours to start the sludge. Activating the composite microbial inoculum by seed liquid, taking 5L of seed liquid at 8000rpm, centrifuging for 10min, discarding supernatant, putting the thalli into a device, adding 12g of sodium acetate as a carbon source every day, setting aeration and stirring time for two hours, one group (90min aeration, 30min stirring), and continuously circulating. Measuring ammonia nitrogen, nitrate nitrogen, nitrite nitrogen, COD and the like of the water sample every day. Discharging the sewage which reaches the standard after degradation through a drain valve, adding the high ammonia nitrogen wastewater again (without adding the composite microbial inoculum), and repeating the three times to verify that the composite microbial inoculum CCZU C6 can continuously survive on the sludge and carry out ammonia nitrogen degradation on the wastewater. The results are shown in FIG. 9.
This example illustrates that after 48 hours of adding the complex microbial inoculum, the ammonia nitrogen is reduced from the initial 155.8mg/L to 0.9mg/L, and the ammonia nitrogen degradation rate reaches 77.4 mg/L.d-1The ammonia nitrogen removal rate reaches 99 percent and is far higher than the ammonia nitrogen degradation rate of the experiment shake flask treatment. This is probably because the amplification effect of the pilot plant makes the combined bacterial agent and other fungus synergism in the mud, has accelerated the ammonia nitrogen degradation rate. Nitrite can not be accumulated in the two previous pilot-scale tests and is continuously converted into nitrate nitrogen, COD content is obviously reduced, and the number of bacteria in the device is greatly increased. The pilot plant test reaches an ideal target, and results of three repeated tests show that the composite microbial inoculum CCZU C6 can stably survive in the activated sludge of the A/O pool, and the ammonia nitrogen degradation rate is stable, so that the method has high popularization and practical application values.
Claims (6)
1. An ammonia oxidation complex microbial inoculum is characterized in that the complex microbial inoculum is prepared from Paracoccus sp.TD-10 of denitrificans Paracoccus with the preservation number of CGMCC NO. 19059; TD-46 of Gordonia sp, with the preservation number of CGMCC NO. 19057; alcaligenes faecalis sp.TD-94, with the preservation number of CGMCC NO. 19058.
2. The method for preparing the ammonia oxidation complex bacterial agent according to claim 1, which comprises the following specific steps:
(1) compounding three strains of efficient ammonia oxidizing bacteria including paracoccus denitrificans TD-10, gordonia bacteria TD-46 and alcaligenes faecalis TD-94 to construct a composite microbial inoculum;
(2) and (3) controlling the carbon source, nitrogen source and C/N ratio of the composite microbial inoculum, and the initial pH, temperature and rotating speed degradation conditions to obtain the efficient ammonia oxidation composite microbial inoculum CCZU C6.
3. The preparation method of the efficient ammonia oxidation complex bacterial agent according to claim 2, wherein the method for constructing the complex bacterial agent in the step (1) comprises the following steps: activating three high-efficiency ammonia oxidizing bacteria in a seed culture medium for 24 hours, designing a compounding ratio by taking the inoculation amount of 1%, 2% and 4% of each strain as a variable, preparing a compound microbial inoculum according to the compounding ratio, transferring the compound microbial inoculum into an optimized basic culture medium, performing shake flask culture at 28 ℃, and rotating at 160rpm for 2 days, measuring the ammonia nitrogen content in the culture medium, selecting a combination with the lowest ammonia nitrogen residual content, and determining the optimal compounding.
4. The method for preparing the efficient ammonia oxidation complex bacterial agent according to claim 3, wherein a seed culture medium comprises: 10g of peptone, 5g of yeast powder, 10g of NaCl, 1L of distilled water and 7.0-7.4 of pH; optimizing a basic culture medium: NH (NH)4Cl 0.382g，CH3COONa 2g，MgSO4·7H2O 0.05g，K2HPO4 0.2g，NaCl 0.12g，MnSO4·4H2O 0.01g，FeSO4·7H2O0.01 g, distilled water 1L, pH 7.0-7.4.
5. The method for preparing the efficient ammonia oxidation complex bacterial agent according to claim 2, wherein the degradation conditions in the step (2) are as follows: selecting glucose, sucrose, sodium acetate, sodium succinate, sodium citrate and potassium sodium tartrate as alternative carbon sources; selecting 100-500mg/L ammonium chloride as a nitrogen source; the C/N ratio ranges from 2 to 18; the initial pH value is 5.2-9.2; the temperature range is 24-34 ℃; the rotation speed range is between 140 and 220 rpm.
6. The application of the efficient ammonia oxidation complex bacterial agent as claimed in claim 1 in coal-to-hydrogen high ammonia nitrogen wastewater.
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| CN113817636A (en) * | 2021-09-22 | 2021-12-21 | 哈尔滨工业大学 | High-temperature-resistant nitrifying bacteria for reducing ammonia emission in sludge composting process and application thereof |
| CN114410506A (en) * | 2021-12-20 | 2022-04-29 | 南京瑞特莱生态环境有限公司 | Alcaligenes faecalis strain and application thereof |
| CN114410506B (en) * | 2021-12-20 | 2023-08-04 | 南京瑞特莱生态环境有限公司 | Alcaligenes faecalis strain and application thereof |
| CN115490553A (en) * | 2022-10-19 | 2022-12-20 | 内蒙古蒙兴源生物科技有限公司 | Cow dung organic fertilizer and preparation method thereof |
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