CN113444661B - Sphingobacterium neoformans and application thereof in wastewater dephosphorization - Google Patents
Sphingobacterium neoformans and application thereof in wastewater dephosphorization Download PDFInfo
- Publication number
- CN113444661B CN113444661B CN202110689092.9A CN202110689092A CN113444661B CN 113444661 B CN113444661 B CN 113444661B CN 202110689092 A CN202110689092 A CN 202110689092A CN 113444661 B CN113444661 B CN 113444661B
- Authority
- CN
- China
- Prior art keywords
- phosphorus
- sjb007
- strain
- sphingosine
- novosphingobium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/105—Phosphorus compounds
Abstract
The invention relates to a new sphingosine bacillus and application thereof in dephosphorization of wastewater. The strain is new sphingosine bacillus (Novosphingobium sp.) SJB007 and is preserved in China general microbiological culture Collection center of China Committee for culture Collection of microorganisms with the preservation number of CGMCC No. 21177. The invention also provides application of the novel sphingosine bacillus SJB007 in removing phosphorus in sewage. The method comprises the following steps: (1) and (3) strain culture: inoculating a preservation strain of sphingosine bacillus SJB007 into a seed culture medium, culturing at 30 ℃ and 160rpm for 24 hours, centrifuging to obtain thalli, and washing the thalli with sterile water to prepare a bacterial suspension with OD600 of 0.50-0.60; (2) and (3) fermentation dephosphorization: inoculating the bacterial suspension prepared in the step (1) into phosphorus-containing sewage in a proportion of 2%, and fermenting under the conditions that the pH is 4-8 and the temperature is 15-35 ℃ to remove phosphorus. The high-efficiency polyphosphate bacterial strain, namely the sphingobacterium neosphingosine (Novosphingobium sp.) SJB007 has the removal rate of over 97 percent under the appropriate conditions when the phosphorus concentration in sewage is 10-30mg/L, and has higher removal rate.
Description
Technical Field
The invention belongs to the technical field of environmental microorganisms, and particularly relates to a novel sphingosine bacillus and application thereof in dephosphorization of wastewater.
Background
The overgrowth of algae caused by the eutrophication of water environment is one of the main problems causing the deterioration of water quality, phosphorus is an important factor causing the eutrophication of water body, the pollution source is mainly domestic, industrial and agricultural wastewater, and therefore one of the main purposes of wastewater treatment is to remove the phosphorus in the wastewater.
The traditional physical and chemical phosphorus removal process has low efficiency and complex technology and is easy to cause secondary pollution, and the biological phosphorus removal method has the characteristics of low cost, small workload, high efficiency, wide application range and the like, and is the main method for removing phosphorus from sewage at present. In biological Phosphorus removal, the main effect is that the Phosphorus-Accumulating bacteria (Phosphorus Accumulating bacteria) which can cause anaerobic Phosphorus release and aerobic or anaerobic/anoxic excessive Phosphorus absorption under the alternate operation of anaerobic/aerobic or anaerobic/anoxic, the Phosphorus-Accumulating bacteria in the microorganisms exceed the Phosphorus-Accumulating amount of common bacteria by several times, the current researches consider that the Phosphorus-Accumulating bacteria are divided into two types of aerobic Phosphorus-Accumulating bacteria and denitrifying Phosphorus-Accumulating bacteria, and the reported biological Phosphorus-removing strains mainly comprise pseudomonads (Zhuweiqiang, Chengshu, Zpayte Yu. 2 strains of denitrifying Phosphorus-Accumulating bacteria and influence factors thereof. environmental engineering reports, 2016, 10(4):3295-, The genus acinetobacter (aceveedo B,
c, Corona J E, et al, the metabolic conversion of PAOs as an opportunity to an object a high level P-engineered stream for furcher P-recovery chemical Engineering Journal, 2015, 270:459-467), Aeromonas, Pseudomonas, Streptococcus, Moraxella and Micrococcus (Ru ping, Cheng, Yang Xin Yan, etc. the functional microbiological research of the enhanced biological phosphorus removal system has progressed, biological technology reports, 2017,33(10):1-8), but most of these strains have the disadvantages of poor tolerance to the natural environment, difficult propagation culture, easy deterioration of activity, etc., and in actual use, the number of viable bacteria is not high, most of the strains do not exhibit good phosphorus removal in the actual process, and the phosphorus removal effect is to be further improved.
Disclosure of Invention
In order to solve the technical problems, the invention provides a high-efficiency phosphorus-accumulating sphingosine bacillus, which is obtained by separating activated sludge from domestic sewage of the university of Wenzhou campus, Wenzhou, Zhejiang by using a conventional separation method, determining a 16SrDNA sequence, comparing and analyzing a sequencing result by GeneBank Blast, determining that the strain has 99 percent of homology with Novosphingobium sp.BH-4(MG855668.1) of the sphingosine bacillus, and determining that the strain belongs to Novosphingobium sp.SJB007.
The high-efficiency phosphorus-accumulating sphingosine bacillus is sphingosine bacillus (Novosphingobium sp.) SJB007 which is preserved in the China general microbiological culture Collection center of the China Committee for culture Collection of microorganisms, and the registration number of the preservation center is CGMCC No. 21177.
The invention also provides application of the novel sphingosine bacillus SJB007 in removing phosphorus in sewage.
The method for removing phosphorus in sewage by using novel sphingosine bacillus SJB007 comprises the following steps:
(1) and (3) strain culture: inoculating a preservation strain of sphingosine bacillus SJB007 into a seed culture medium, culturing at 30 ℃ and 160rpm for 24 hours, centrifuging to obtain thalli, and washing the thalli with sterile water to prepare a bacterial suspension with OD600 of 0.50-0.60;
(2) and (3) fermentation dephosphorization: inoculating the bacterial suspension prepared in the step (1) into phosphorus-containing sewage in a proportion of 2%, and fermenting under the conditions that the pH is 4-8 and the temperature is 15-35 ℃ to remove phosphorus.
Wherein the seed culture medium in the step (1) comprises the following components: yeast powder 1.0g, NaCl 1.0g, KH2PO4 0.3g、K2HPO4 0.25g、MgSO4·7H20.2g of O and 1.0g of glucose, wherein the volume of distilled water is up to 1000mL, and the pH value is adjusted to 7.0 by 1mol/L NaOH aqueous solution and/or 1mol/L HCl.
Preferably, the culture temperature for removing the phosphorus-containing sewage in the step (2) is 25-30 ℃, and the pH is adjusted to 5-8 by NaOH or HCl.
Preferably, in the step (2), the bacterial suspension obtained in the step (1) is inoculated into phosphorus-containing sewage and cultured at the rotating speed of 160-.
The invention can achieve the following technical effects:
1. the efficient polyphosphate-accumulating bacterial strain SJB007 is firstly reported in China.
2. The efficient phosphorus-accumulating bacterial strain, namely the sphingosine bacillus sp (Novosphingobium sp.) SJB007 has the appropriate temperature of 15-35 ℃ for removing phosphorus in phosphorus-containing sewage, and has a relatively wide temperature adaptation range.
3. The high-efficiency polyphosphate bacterial strain, namely the sphingobacterium neosphingosine (Novosphingobium sp.) SJB007 has the removal rate of over 97 percent under the appropriate conditions when the phosphorus concentration in sewage is 10-30mg/L, and has higher removal rate.
4. The efficient polyphosphate accumulating strain, namely the sphingosine bacillus (Novosphingobium sp.) SJB007 has wide adaptability and good dephosphorization effect under aerobic conditions, and has great application potential in actual wastewater phosphorus removal.
Drawings
FIG. 1 is a colony of Sphingobacterium neoformans (Novosphingobium sp.) SJB 007.
FIG. 2 shows phylogenetic trees of strain SJB007 and the 16S rDNA sequence of related species using MEGA4.1 software, ortho ligation.
Strain preservation
The novel sphingosine bacillus (Novosphingobium sp.) SJB007 has been deposited in the China general microbiological culture Collection center of China Committee for culture Collection of microorganisms at the deposition address: no. 3 Xilu No. 1 Beijing, Chaoyang, the registration number of the preservation center is CGMCC No.21177, and the preservation start date is 11/13/2020.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail with reference to the accompanying drawings.
The invention provides a high-efficiency phosphorus-accumulating novel sphingosine bacillus, which is obtained by separating activated sludge collected from campus domestic sewage of Wenzhou university in Wenzhou, Zhejiang by adopting a conventional separation method, is determined by a 16SrDNA sequence, is subjected to comparison analysis by GeneBank Blast, has 99 percent of homology with Novosphingobium sp.BH-4(MG855668.1) of the novel sphingosine bacillus, and is determined to belong to Novosphingobium sp.SJB007.
The experimental methods without specifying specific conditions in the following examples were selected according to the conventional methods and conditions, or according to the commercial instructions.
The strain media used in the examples were as follows:
enrichment medium and seed medium: yeast powder 1.0g, NaCl 1.0g, KH2PO4 0.3g、K2HPO4 0.25g、MgSO4·7H20.2g of O, 1.0g of glucose and distilled water to 1000mL, and the pH value is 7.0.
Separating a culture medium: yeast powder 1.0g, NaCl 1.0g, KH2PO4 0.3g、K2HPO4 0.25g、MgSO4·7H20.2g of O, 1.0g of glucose, 20.0g of agar and distilled water to 1000mL, and the pH value is 7.0.
Polyphosphate granule staining medium: na (Na)3C6H5O7·2H2O 4.0g、NaCl 0.5g、(NH4)2SO4 2.5g、CaCl2 0.25g、MgSO4·7H2O 0.25g、Na2HPO4 12.8g、KH2PO43g, maltose 0.01g and toluidine blue O0.025 g, distilled water to 1000mL, pH7.0.
Phosphorus-accumulating culture medium: glucose 10g, (NH)4)2SO4 0.5g、KCl 0.3g、NaCl 0.3g、FeSO4·7H2O 0.03g、MnSO4·H2O 0.03g、CaCO35g and KH2PO40.0439g, distilled water to 1000mL, pH7.0.
Example 1 isolation and identification of novel Sphingobacterium sp (Novosphingobium sp.) SJB007
First, separating and screening bacterial strain
The sample is activated sludge from domestic sewage of Wenzhou university campus in Wenzhou, Zhejiang province, and a certain amount of activated sludge is added into a 250mL conical flask filled with 100mL of enrichment medium according to 10% by adopting a conventional separation method, and is subjected to shaking culture at 30 ℃ and 160rpm for one week. Then coating the diluted bacterial liquid on a separation culture medium, culturing for 48 hours at 30 ℃, picking a single colony, transferring the single colony to a new separation culture medium, and performing streak purification until the single colony is a pure culture through microscopic examination. The cells were stored at 4 ℃ after transfer.
The deposited strains were inoculated into seed cultures at 30 ℃ and 150r/min (100 mL in 250mL conical flasks) for 24 hours. Then inoculating in
mu.L of polyphosphate granular staining medium and 20. mu.L of the bacterial suspension were added to a 96-well plate, and the same amount of sterile water was added to the control group. Culturing at 30 ℃ for 48h, if polyphosphate particles are formed in the test strain, toluidine blue enters the bacteria to react with the bacteria for coloring, the concentration of the toluidine blue in the pores is reduced, the color is lightened, and the more obvious color fading indicates that the bacterial strain has stronger phosphorus-gathering capability. The polyphosphate strain capable of causing the toluidine blue to fade most remarkably was selected according to the color change and named as SJB007, and further purified, and the colony morphology thereof is shown in fig. 1. As can be seen from FIG. 1, the colonies are round, have neat edges, are yellow and opaque, have smooth, moist and sticky surfaces, and are convex in the middle.
II, molecular identification of strains
Culturing the target strain on a slant culture medium for 24 hours, extracting genome DNA, amplifying the 16S rDNA of the strain by utilizing a PCR technology, and using a primer, a forward primer (5'-CAGAGTTTGATCCTGGCT-3') and a reverse primer (5'-AGGAGGTGATCCAGCCGCA-3');
the PCR reaction system had the following composition:
the PCR conditions are shown in the following table:
5. mu.L of the PCR product was subjected to electrophoresis with 1% agarose (150V, 100mA, 20min) and the amplified fragment was found to be about 1.5kb in length as a pass. The PCR product qualified for detection was purified using a SanPrep column PCR product purification kit and sequenced by Biotechnology engineering (Shanghai) Inc. The 16S rDNA sequence was compared with 16S rDNA in GenBank database, and as a result, as shown in FIG. 2, it was found that the highest homology was 99% with Novosphingobium sp.BH-4(GenBank accession No. MG855668.1), which was determined to be Novosphingobium sp.coded as Novosphingobium sp.SJB007.
Example 2 phosphorus removal Properties of Novosphingobium sp.SJB007
Inoculating the preserved strain into a 250mL conical flask filled with 100mL of seed culture medium, culturing at 30 ℃ for 24h at 150r/min, centrifuging at 5000r/min for 10min, washing with sterile water for 2 times, and preparing into bacterial suspension (OD600 is 0.5-0.6) with sterile water; then transferring the mixture into a 250mL conical flask filled with 100mL of phosphorus-accumulating culture medium according to the volume ratio of 2 percent, culturing for 24h at a certain temperature and rotating speed, then centrifuging for 10min at 14000r/min, collecting the supernatant, and determining the phosphorus concentration by adopting a molybdenum-antimony anti-spectrophotometry method specified in GB 11893-1989. The phosphorus removal rate was calculated using the following formula:
according to the method, the influence of pH, temperature, the rotating speed of a shaking table and phosphorus concentration on phosphorus removal of the Novosphingobium sp.SJB007 by the sphingosine neomycin is mainly researched.
1. Selection of pH
Preparing a phosphorus-accumulating culture medium with the phosphorus concentration of 10mg/L, adjusting the pH value to 4, 5, 6, 7, 8, 9 and 10 respectively, inoculating 2 percent (volume ratio) of seed solution, culturing at 30 ℃ and 160rpm for 24h, centrifuging for 10min at 14000r/min, collecting supernatant, and measuring the phosphorus concentration, wherein the results are shown in the following table. As can be seen from the table, the strain SJB007 has good effect of removing phosphorus from sewage within the pH value range of 4-8, and the removal rate exceeds 97%.
| pH | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
| Phosphorus removal Rate (%) | 97.713 | 98.140 | 98.980 | 99.827 | 98.140 | 62.117 | 3.636 |
2. Selection of culture temperature
Preparing a phosphorus-accumulating culture medium with the phosphorus concentration of 10mg/L, adjusting the pH value to 7, inoculating 2 percent (volume ratio) of seed liquid, culturing for 24h at the temperature of 15, 20, 25, 30 and 35 ℃ and at 160rpm, centrifuging for 10min at 14000r/min, collecting supernatant, and measuring the phosphorus concentration, wherein the results are shown in the following table. As can be seen from the table, the strain SJB007 has good effect of removing phosphorus from sewage at the temperature of 15-35 ℃ and the removal rate exceeds 97%.
| Temperature (. degree.C.) | 15 | 20 | 25 | 30 | 35 |
| Phosphorus removal Rate (%) | 98.983 | 97.713 | 99.407 | 98.983 | 98.133 |
3. Selection of the rotational speed of the rocking platforms
Preparing a phosphorus-accumulating culture medium with the phosphorus concentration of 10mg/L, adjusting the pH value to 7, inoculating 2 percent (volume ratio) of seed liquid, culturing for 24h at the conditions of 80, 120, 160 and 200rpm and 30 ℃, centrifuging for 10min at 14000r/min, collecting supernatant, and measuring the phosphorus concentration, wherein the results are shown in the following table. As can be seen from the table, the strain SJB007 has good effect of removing phosphorus from sewage in the rotating speed range of 80-200r/min, and the removal rate exceeds 97%.
| Shaking table rotating speed (r/min) | 80 | 120 | 160 | 200 |
| Phosphorus removal Rate (%) | 97.713 | 97.713 | 98.983 | 98.133 |
4. Phosphorus concentration test in wastewater
Preparing phosphorus-accumulating culture media with different phosphorus concentrations of 10, 20, 30, 40, 50, 60 and 70mg/L respectively, adjusting the pH value of the phosphorus-accumulating culture media to 7, inoculating 2 percent (volume ratio) of seed liquid, culturing for 24 hours at the conditions of 160rpm and 30 ℃, centrifuging for 10 minutes at 14000r/min, collecting supernatant, and determining the phosphorus concentration, wherein the results are shown in the following table. As can be seen from the table, the strain SJB007 has good effect of removing phosphorus from sewage within the phosphorus concentration range of 10-30mg/L, and the removal rate exceeds 97%.
Example 3 Effect of Novosphingobium sp.SJB007 of Novosphingomonas sp.SJB007 on removal of phosphorus from anaerobically treated piggery wastewater
A suspension of Novosphingobium sp.SJB007, which is a novel sphingosine strain, was prepared according to the method of example 2, and was transferred to 200mL of anaerobically treated piggery wastewater containing the suspension at a volume ratio of 2%, and the wastewater was cultured at 25 ℃ and 160r/min, using the original wastewater without the suspension as a control. After 24h of incubation, the cells were centrifuged at 14000r/min for 10min, the supernatant was collected and the phosphorus concentration was determined. The phosphorus concentration of the piggery wastewater after anaerobic treatment is 3.12mg/L, the phosphorus concentration of the wastewater is reduced to 1.57 after the wastewater is cultured by the Novosphingobium sp.SJB007, and the removal rate is 49.68 percent; the phosphorus concentration of the control group is 2.91mg/L, and the phosphorus concentration reduction rate is 6.73%. The strain Novosphingobium sp.SJB007 has application potential of removing actual phosphorus-containing wastewater.
Sequence listing
<110> university of Wenzhou
<120> a new sphingosine bacillus and application thereof in wastewater dephosphorization
<130> 2021
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1424
<212> DNA
<213> novel Sphingobacterium sp (Novosphingobium sp.)
<400> 1
ggctcagaac gaacgctggc ggcatgccta acacatgcaa gtcgaacgag atcttcggat 60
ctagtggcgc acgggtgcgt aacgcgtggg aatctgccct tgggttcgga ataacagtga 120
gaaattactg ctaataccgg atgatgtctt cggaccaaag atttatcgcc cagggatgag 180
cccgcgtagg attaggtagt tggtggggta atggcctacc aagccgacga tccttagctg 240
gtctgagagg atgatcagcc acactgggac tgagacacgg cccagactcc tacgggaggc 300
agcagtgggg aatattggac aatgggcgca agctgatcca gcaatgccgc gtgagtgatg 360
aaggctttag ggttgtaaag ctcttttacc agggatgata atgacagtac ctggagaata 420
agctccgggc taactccgtg ccagcagccg cggtaatacg gagggagcta gcgttgttcg 480
gaattactgg gcgtaaagcg cgcgtaggcg gttactcaag tcagaggtga aagcccgggg 540
ctcaaccccg gaactgcctt tgaaactagg tgactagaat cttggagagg tcagtggaat 600
tccgagtgta gaggtgaaat tcgtagatat tcggaagaac accagtggcg aaggcgactg 660
actggacaag tattgacgct gaggtgcgaa agcgtgggga gcaaacagga ttagataccc 720
tggtagtcca cgccgtaaac gatgataact agctgtccgg gtacttggta cttgggtggc 780
gcagctaacg cattaagtta tccgcctggg gagtacggtc gcaagattaa aactcaaagg 840
aattgacggg ggcctgcaca agcggtggag catgtggttt aattcgaagc aacgcgcaga 900
accttaccag cgtttgacat gccggtcgcg gatttgggag accatttcct tcagttcggc 960
tggaccgtgc acaggtgctg catggctgtc gtcagctcgt gtcgtgagat gttgggttaa 1020
gtcccgcaac gagcgcaacc ctcgtcctta gttgccagca tttagttggg cactctaagg 1080
aaactgccgg tgataagccg gaggaaggtg gggatgacgt caagtcctca tggcccttac 1140
acgctgggct acacacgtgc tacaatggcg gtgacagtgg gcagcaagca ggcgactgca 1200
agctaatctc caaaagccgt ctcagttcgg attgttctct gcaactcgag agcatgaagg 1260
cggaatcgct agtaatcgcg gatcagcatg ccgcggtgaa tacgttccca ggccttgtac 1320
acaccgcccg tcacaccatg ggagttggat tcactcgaag gcgttgagct aacccgcaag 1380
ggaggcaggc gaccacagtg ggtttagcga ctgggtgaag tcga 1424
Claims (4)
1. A new sphingosine bacillus is characterized in that the new sphingosine bacillus (Novosphingobium sp.) SJB007 is preserved in the China general microbiological culture Collection center of China Committee for culture Collection of microorganisms, the accession number of the preservation center is CGMCC No.21177, and the preservation date is 2020, 11 months and 13 days.
2. The use of the novel bacterium sphingosine according to claim 1, wherein the novel bacterium sphingosine SJB007 is inoculated into phosphorus-containing wastewater and cultured to remove phosphorus.
3. The use of the novel bacterium sphingosine according to claim 2, comprising the steps of:
(1) and (3) strain culture: inoculating a preservation strain of sphingosine bacillus SJB007 into a seed culture medium, culturing at 30 ℃ and 160rpm for 24 hours, centrifuging to obtain thalli, and washing the thalli with sterile water to prepare a bacterial suspension with OD600 of 0.50-0.60;
(2) and (3) fermentation dephosphorization: inoculating the bacterial suspension prepared in the step (1) into phosphorus-containing sewage in a proportion of 2%, and fermenting under the conditions that the pH is 4-8 and the temperature is 15-35 ℃ to remove phosphorus.
4. The use of the bacterium sphingosine according to claim 3, wherein the seed culture medium consists of: yeast powder 1.0g, NaCl 1.0g, KH2PO4 0.3g、K2HPO4 0.25g、MgSO4·7H20.2g of O and 1.0g of glucose, wherein the volume of distilled water is up to 1000mL, and the pH value is adjusted to 7.0 by 1mol/L NaOH aqueous solution and/or 1mol/L HCl.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110689092.9A CN113444661B (en) | 2021-06-21 | 2021-06-21 | Sphingobacterium neoformans and application thereof in wastewater dephosphorization |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110689092.9A CN113444661B (en) | 2021-06-21 | 2021-06-21 | Sphingobacterium neoformans and application thereof in wastewater dephosphorization |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113444661A CN113444661A (en) | 2021-09-28 |
| CN113444661B true CN113444661B (en) | 2022-03-04 |
Family
ID=77812124
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110689092.9A Active CN113444661B (en) | 2021-06-21 | 2021-06-21 | Sphingobacterium neoformans and application thereof in wastewater dephosphorization |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113444661B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113717902B (en) * | 2021-10-11 | 2022-10-04 | 中国热带农业科学院热带作物品种资源研究所 | Sphingobacterium multivorum and application thereof |
| CN113755401B (en) * | 2021-10-11 | 2023-04-07 | 中国热带农业科学院热带作物品种资源研究所 | New sphingosine bacillus Novosphingobium capsulatum and application thereof |
| CN115072880B (en) * | 2021-11-29 | 2024-01-26 | 江苏宜裕环保科技有限公司 | Biological flocculant and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003180341A (en) * | 2001-12-25 | 2003-07-02 | Sumitomo Forestry Co Ltd | Bacterium having aromatic compound decomposition activity and method for producing the same |
| CN109402016A (en) * | 2018-09-21 | 2019-03-01 | 江苏宜裕环保科技有限公司 | For the complex micro organism fungicide of chemical wastewater treatment and its screening and preparation method |
| CN110563132A (en) * | 2019-09-05 | 2019-12-13 | 重庆大学 | Aerobic granular sludge culture method |
| CN112553113A (en) * | 2020-12-18 | 2021-03-26 | 青岛根源生物技术集团有限公司 | Clavus new sphingosine bacillus GBW-HB1906 with broad-salt resistance and application thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA3011788A1 (en) * | 2016-01-19 | 2017-07-27 | Bioconsortia, Inc. | Agriculturally beneficial microbes, microbial compositions, and consortia |
| SE1650321A1 (en) * | 2016-03-09 | 2017-06-27 | Veolia Water Solutions & Tech | Biological removal of micropollutants from wastewater |
-
2021
- 2021-06-21 CN CN202110689092.9A patent/CN113444661B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003180341A (en) * | 2001-12-25 | 2003-07-02 | Sumitomo Forestry Co Ltd | Bacterium having aromatic compound decomposition activity and method for producing the same |
| CN109402016A (en) * | 2018-09-21 | 2019-03-01 | 江苏宜裕环保科技有限公司 | For the complex micro organism fungicide of chemical wastewater treatment and its screening and preparation method |
| CN110563132A (en) * | 2019-09-05 | 2019-12-13 | 重庆大学 | Aerobic granular sludge culture method |
| CN112553113A (en) * | 2020-12-18 | 2021-03-26 | 青岛根源生物技术集团有限公司 | Clavus new sphingosine bacillus GBW-HB1906 with broad-salt resistance and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 解磷菌Novosphingobium sp.YF20的分离及生长特性;孙会忠等;《基因组学与应用生物学》;20171225;第36卷(第12期);5173-5178 * |
| 高效聚磷鞘氨醇杆菌XF-5的分离与鉴定;李海峰等;《河南农业科学》;20120930;第41卷(第9期);68-72 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113444661A (en) | 2021-09-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN113444661B (en) | Sphingobacterium neoformans and application thereof in wastewater dephosphorization | |
| CN109182178B (en) | Strain with chromium tolerance and Cr (VI) removal capacity and application thereof in-situ remediation of moderately and slightly chromium-polluted soil | |
| EP3800243B1 (en) | Bacterium degrading las and n and use thereof | |
| US11731892B2 (en) | Novosphingobium sp. SJB007 and application thereof in removal of phosphorus from wastewater | |
| CN113174345A (en) | Heterotrophic nitrification-aerobic denitrification strain for efficient denitrification and application thereof | |
| CN114908016B (en) | Pseudomonas denitrificans and application thereof in field of sewage and wastewater purification | |
| CN108504585B (en) | Benzene degrading bacterium for treating atmospheric pollution and preparation method and application thereof | |
| CN114045239B (en) | Paracoccus ubiquitously YBH-7 with dimethylacetamide degradation capability and application thereof | |
| CN113249273B (en) | Salt-tolerant bacillus subtilis and application thereof in high-salt ammonia nitrogen wastewater treatment | |
| CN113462622B (en) | Pseudomonas for efficiently degrading various aromatic pollutants and application thereof | |
| CN110656071A (en) | Paracoccus huilkii for efficiently degrading DMF (dimethyl formamide) and application thereof | |
| CN111378592B (en) | Bacillus licheniformis and method for treating malodorous organic wastewater by using same to purify water | |
| CN114292798B (en) | Anaerobic denitrifying strain and application thereof in riverway water body remediation | |
| CN114196590B (en) | Pseudomonas secreting lipase and application thereof in kitchen wastewater treatment | |
| CN114045238B (en) | Rhodococcus ruber HJM-8 capable of efficiently degrading dimethylacetamide and application thereof | |
| CN112300961B (en) | Quinoline degrading strain and application thereof in quinoline-containing wastewater | |
| CN113980851A (en) | Paracoccus YBH-X with dimethylacetamide degradation capability and application thereof | |
| CN111621437B (en) | Otter escherichia coli LM-DK separated from oxidation pond of pig farm and application thereof | |
| CN108441437B (en) | Complex microbial inoculant and application thereof | |
| CN113416681A (en) | Low-carbon and high-nitrogen resistant heterotrophic nitrification-aerobic denitrification bacterium and application thereof | |
| CN112048451A (en) | Citrobacter and application thereof in sulfate-containing wastewater treatment and citric acid bacillus separation and identification method | |
| CN114940957B (en) | Paracoccus ubitus with facultative denitrification synchronous denitrification and dephosphorization performances | |
| CN113800652B (en) | Salt-tolerant aerobic denitrifying bacterium and application of coupling activated carbon thereof in strengthening water body pollution treatment | |
| CN112501080B (en) | Application of sphingosine bacillus clarfungi in preparation of microbial agent for removing COD (chemical oxygen demand) in petroleum wastewater | |
| CN114480221B (en) | Equilibrium brevifolium and application thereof in formaldehyde degradation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |