CN110563132A - Aerobic granular sludge culture method - Google Patents
Aerobic granular sludge culture method Download PDFInfo
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- CN110563132A CN110563132A CN201910838030.2A CN201910838030A CN110563132A CN 110563132 A CN110563132 A CN 110563132A CN 201910838030 A CN201910838030 A CN 201910838030A CN 110563132 A CN110563132 A CN 110563132A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
- C02F3/10—Packings; Fillings; Grids
- C02F3/104—Granular carriers
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
- C02F3/12—Activated sludge processes
- C02F3/1236—Particular type of activated sludge installations
- C02F3/1263—Sequencing batch reactors [SBR]
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/004—Apparatus and plants for the biological treatment of water, waste water or sewage comprising a selector reactor for promoting floc-forming or other bacteria
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Abstract
The invention discloses a high-efficiency and high-strength aerobic granular sludge culture method based on a biological enhancement technology, which is characterized in that strains with higher quorum sensing signal molecule generation capacity are separated, identified and screened out from mature aerobic granular sludge, enrichment culture is carried out, bacterial liquid in a logarithmic phase is mixed in equal volume, and a signal molecule generating bacteria mixed bacterial liquid for biological enhancement is prepared; in the initial stage of aerobic granular sludge culture, regularly and quantitatively adding mixed bacteria liquid to increase the concentration of signal molecules in a system; and stopping adding the mixed bacteria liquid after the aerobic granular sludge is completely granulated. The aerobic granular sludge cultured by the method has short formation period, large grain diameter, high strength and difficult stagnation, and can efficiently and stably remove pollutants in sewage. The invention applies a quorum sensing mechanism and a biological enhancement technology, is a simple, efficient, economic and sustainable aerobic granular sludge culture method, and can improve the practical applicability of an aerobic granular sludge process.
Description
Technical Field
The invention relates to the technical field of biological wastewater treatment, in particular to a high-efficiency and high-strength aerobic granular sludge culture method based on a biological enhancement technology.
Background
aerobic granular sludge is a special biofilm formed by the self-coagulation of microorganisms. Compared with the traditional activated sludge method, the aerobic granular sludge has the advantages of compact structure, good sedimentation performance, high biomass, capability of realizing synchronous nitrogen and phosphorus removal, strong impact load resistance, small occupied area and the like. However, the aerobic granular sludge generally has the disadvantages of long formation period (20-50d), poor granule stability, easy granule disintegration and the like, so that the rapid formation and the stable operation of the system are promoted to be the important research points in the field.
Microbial quorum sensing is a widespread means of communicating information between cells. The method is characterized in that the density of surrounding flora can be sensed by detecting the concentration of signal molecules in the environment among bacteria, and when the density of the flora reaches a certain threshold value, the expression of one or more genes can be started, so that a series of population behaviors including cell directed growth, synthesis of extracellular metabolites, bioluminescence, antibiotic secretion, biofilm formation and the like can be regulated and controlled. Currently known signaling molecules fall into three categories: N-Acyl Homoserine Lactones (AHLs) acting between gram-negative bacteria, oligopeptides acting between gram-positive bacteria, and a second type of signal molecules (AI-2) acting between gram-negative bacteria and gram-positive bacteria.
Most of the functional bacteria in the aerobic granular sludge system are gram-negative bacteria, so the signal molecules AHLs are the main communication languages for the mutual communication among cells. In the initial stage of aerobic granular sludge culture, if the concentration of signal molecules AHLs in the system is regulated and controlled by applying a quorum sensing theory, the aggregation of microorganisms can be promoted, the secretion of extracellular polymeric substances and the formation of aerobic granular sludge are accelerated, but if the signal molecules are directly added, the long-term positive effect cannot be realized because the signal molecules are easily degraded, and in addition, the high cost also limits the wide application of the method.
Therefore, the invention adopts the biological enhancement technology to directly introduce the quorum sensing signal molecule AHLs producing bacteria into the aerobic granular sludge system, enhances the density and information exchange of specific floras in the system, can effectively shorten the formation period of the aerobic granular sludge, and is more economical, stable and sustainable.
Disclosure of Invention
in view of the above, the invention provides a high-efficiency and high-strength aerobic granular sludge culture method based on a biological enhancement technology, by using the method, complete granulation can be realized in a short time (15-20d), and the formed granular sludge has a compact structure and high strength, and is beneficial to maintaining the stability of process operation. The method is simple to operate, low in cost and easy to implement.
In order to achieve the purpose, the invention adopts the following technical scheme:
The invention discloses a high-efficiency and high-strength aerobic granular sludge culture method based on a biological enhancement technology, which comprises the following specific operation steps:
(1) selecting mature aerobic granular sludge for ultrasonic crushing, and separating bacteria by adopting a dilution flat plate coating method;
(2) sequentially numbering the single colonies with obvious morphological differences obtained by culturing in the step (1), selecting the single colonies, performing streak culture, and repeatedly executing the step for 3 times;
(3) Transferring the purified strain in the step (2) to a culture medium containing biological reporter bacteria, screening out signal molecule producing bacteria, re-screening for 2 times, and then carrying out 16s RNA sequencing comparison to identify the bacterial species;
(4) Selecting 7 strains of bacteria from the identified strains for enrichment culture, mixing bacterial liquids in logarithmic phase in equal volume, determining the type of signal molecule generation, and using the mixed bacterial liquid of the signal molecule generating bacteria for biological enhancement;
(5) In the initial stage of culturing aerobic granular sludge in the SBR reactor, regularly and quantitatively adding a mixed bacterial liquid of signal molecule producing bacteria into the reactor, namely a biological strengthening process;
(6) Stopping adding the signal molecule producing bacteria mixed bacterial liquid after the aerobic granular sludge is completely granulated;
(7) The SBR reactor is stably operated for 120-200 d.
Preferably, in the step (1), the mature aerobic granular sludge is taken from the SBR reactor which is stably operated for more than 100 days, has the grain diameter range of 2-4mm and has better settling property (SVI)1025-50mL/g) and contaminant removal Capacity (COD, NH)4 +And PO4 3-Removal rates of greater than 90%, and 70%, respectively); the conditions of the ultrasonic crushing of the aerobic granular sludge are as follows: 100-200W, 20-40kHz, 3-10 min; and (3) diluting the bacterial suspension subjected to ultrasonic treatment in a gradient manner by adopting sterile Phosphate Buffer Solution (PBS), uniformly coating the bacterial suspension in an R2A solid culture medium, and culturing for 12-36h at the temperature of 25-30 ℃.
Preferably, in the step (2), a single colony is selected and streaked on an R2A solid medium, and is cultured for 12-18h at the temperature of 25-30 ℃.
Preferably, in the step (3), the bacterium producing the signal molecule N-Acyl Homoserine Lactone (AHL) is screened by using the biological reporter bacterium agrobacterium tumefaciens KYC55, and the specific screening method comprises the following steps: 5-20mL of overnight cultured Agrobacterium tumefaciens KYC55 suspension, 5-20mL of AT buffer solution and 5-20mL of 1-3% water agar are fully mixed, 80-120 mu L of galactoside (X-gal) is added, the mixture is insulated AT 40-50 ℃, the mixture is poured into a culture dish, after the mixture is solidified, the strain to be detected is transferred to a culture medium, the culture is carried out for 12-48h AT 20-30 ℃, the color change of the strain is observed, and the blue strain is the signal molecule producing strain.
Preferably, in the step (4), the selected 7 strains are: sphingomonas sp.SPQ-06, Caulobacter sp.SPQ-12, Caulobacter vitaides strain, Microbacterium azadirachtae, Novosphingobium sp.SPQ-04, Sphingomonas sp.Z-03, Caulobacter sp.SPQ-06, the gene sequence of which has been uploaded to NCBI database, and the obtained Genbank serial numbers of MK602660, MK602661, MK602698, MK602699, MK602700, McK,MK602701, MK 602702; properties of 7 strains: all have high signal molecule generating capacity and fast growth rate (OD is obtained after culturing in R2A liquid culture medium at 25-30 deg.C for 8-15h600More than 0.5), part of the bacteria have better removal capability of COD, N and P; the 7 kinds of bacteria in logarithmic phase are mixed in equal volume, and the mixed bacteria liquid is analyzed by ultra high performance liquid chromatography to generate four types of signal molecules: c6-HSL、3OC8-HSL、C8-HSL and C10-HSL。
Preferably, in the step (5), the inoculated sludge of the SBR reactor is activated sludge of an aeration tank or a sedimentation tank of a municipal sewage treatment plant, the initial MLSS in the reactor is 2-5g/L, and the SVI is3085-150 mL/g; the inflow substrate is simulated urban domestic sewage artificially configured in a laboratory, wherein the COD is 300-700
mg/L，NH4 +Is 30-70mg/L, PO4 3-5-20 mg/L; the operation mode of the SBR reactor is sequentially carried out according to the sequence of water inlet, anaerobic treatment, aeration, precipitation and water discharge, the operation period is 4 hours, wherein the aeration time is 90-180min, the precipitation time is 3-20min, the volume exchange ratio of the water discharge stage is 40-60%, and the SRT is controlled to be 20-30 d; the biological enhancement process comprises mixing bacterial liquid (OD) with signal molecule producing bacteria6000.5-1.5, MLSS 0.1-2g/L) according to the following formula: the proportion (volume ratio) of 100 is added in the water inlet stage.
Preferably, in the step (6), the SBR reactor can realize the complete granulation of the aerobic granular sludge (the average grain diameter is more than 0.3mm, SVI) in 15-20 days30/SVI10＞0.9)。
preferably, in the step (7), the aerobic granular sludge cultured by the method has the average grain diameter of 2-4mm, compact structure, high strength (the integrity coefficient is 95.0-99.5 percent) and good settling property (SVI)1030-50mL/g) and can keep the structural integrity, COD and NH within 120-200d4 +And PO4 3-The removal rate is stabilized at more than 90%, 95% and 70%.
According to the technical scheme, compared with the prior art, the invention has the following beneficial effects:
(1) The 7 kinds of screened quorum sensing signal molecule producing bacteria come from a mature aerobic granular sludge system, and after the screening is successful, the mixed bacteria liquid can be directly added into an SBR reactor, so that the effects of increasing the quorum sensing signal molecule concentration, increasing the extracellular polymer production and further accelerating the formation of aerobic granular sludge are achieved, and the method has good adaptability and high efficiency;
(2) the implementation method is simple, the cost is low, and aerobic granular sludge can be quickly formed only by adding the signal molecule producing bacterium mixed bacterial liquid in proportion to the water inlet substrate in the water inlet stage;
(3) The growth rate and activity of functional bacteria in the aerobic granular sludge cultured by the method are obviously increased, and the aerobic granular sludge has better pollutant removal capacity;
(4) compared with aerobic granular sludge without adding quorum sensing signal molecule generating bacteria, the aerobic granular sludge formed by the method has the advantages of large grain size, compact structure, high strength, good settling property and long-term effect of maintaining the stability of the aerobic granular sludge.
Drawings
in order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 is a diagram showing the screening and re-screening process of a signal molecule-producing strain in example 1 of the present invention, in which (a) and (b) show the color development of a part of the test strains transferred to a solid medium containing a biological reporter and cultured for 24 hours; panel (c) shows bacterial coloration during rescreening.
FIG. 2 is a schematic view of the aerobic granular sludge at 150d according to example 1 of the present invention.
FIG. 3 is a graph showing the morphology of aerobic granular sludge at 150d in comparative example 1.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
example 1
The method comprises the following steps: and preparing a signal molecule producing bacterium mixed bacterium liquid.
(1) Selection of mature aerobic granular sludge: the aerobic granular sludge is taken from a laboratory-scale SBR reactor which stably runs for 120 days, the average grain diameter of the sludge is 2.4mm, and the SVI1042.9 mL/g;
(2) separation of signal molecule producing bacteria: cleaning 10mL of aerobic granular sludge mixed liquor for 3 times by adopting sterile PBS buffer solution, carrying out ultrasonic crushing (100W/20kHz, 5min), uniformly coating the bacteria liquid after gradient dilution on an R2A solid culture medium, and carrying out overnight culture at the temperature of 30 ℃; sequentially numbering the colonies with obvious morphological differences, selecting a single colony to perform streak culture on an R2A solid culture medium, and repeatedly executing the step for 3 times; transferring the bacterial colony to be detected to a solid culture medium containing biological report bacteria Agrobacterium tumefaciens KYC55 and X-gal, culturing at 30 ℃ for 24h to observe the color change of the bacterial strain, wherein the blue bacterial colony is the signal molecule generating strain.
The action principle of adopting the biological reporter KYC55 to detect the signal molecule producing bacteria is as follows: the agrobacterium tumefaciens KYC55 is a high-efficiency sensitive biological detection strain, does not generate signal molecules AHLs by itself, but reacts with signal molecules in the environment in the presence of X-gal to display blue.
(3) Preparing a signal molecule producing bacterium mixed bacterium solution: selecting 7 signal molecule producing bacteria, which are Sphingomonas sp.SPQ-06, Caulobacter sp.SPQ-12, Caulobacter vibrio desmosis toxin, Microbacterium azadirachta, Novosphingobium sp.SPQ-04, Sphingomonas sp.Z-03 and Caulobacter sp.SPQ-06; and (3) mixing the 7 bacterial solutions in the logarithmic growth phase in equal volume to prepare a signal molecule producing bacteria mixed bacterial solution for the biological enhancement process.
Step two: and (5) culturing aerobic granular sludge.
(1) Water quality of inlet water: artificially configuring a water inlet source to simulate urban domestic sewage, wherein: COD is 600mg/L, NH4 +Is 60mg/L, PO4 3-10mg/L, Ca2+30mg/L，Mg2+30mg/L, and 1mL/L of trace elements; the trace elements comprise the following components: FeCl3·6H2O，0.5g/L；H3BO3，0.15g/L；CuSO4·5H2O，0.05g/L；MnCl2·4H2O，0.15g/L；Na2MoO4·2H2O，0.1g/L；ZnSO4·7H2O，0.15g/L；CoCl2·6H2O，0.15g/L；EDTA，0.05g/L；
(2) sludge inoculation: adding flocculent sludge from a municipal sewage treatment plant into artificially prepared domestic sewage for 3d of aeration, and taking a certain amount of domesticated flocculent sludge as inoculated sludge; after inoculation, the initial MLSS in the reactor was 3.6g/L, SVI30107.6 mL/g;
(3) The operation mode of the SBR reactor is as follows: the SBR reactor consists of organic glass, the height is 60cm, the inner diameter is 6cm, and the effective volume is 1.6L; the operation period is 4 hours, the operation period is divided into five stages of water inlet, anaerobic treatment, aeration, precipitation and water drainage, the five stages comprise water inlet 5min, anaerobic treatment 60min, aeration 150-165min, precipitation 5-20min and water drainage 5min, the volume exchange ratio of the water drainage stage is 50%, and the SRT is set to be 30 d;
Step three: a biological strengthening process.
Will OD6000.8, 0.65g/L MLSS signal molecule producing bacteria mixed bacterial liquid and the simulated domestic sewage 3: the proportion (volume ratio) of 100 is added at the same time in the water inlet stage; and stopping adding the signal molecule producing bacteria mixed bacterial liquid after the sludge is completely granulated.
comparative example 1
The aerobic granular sludge culture mode of the comparative example 1 is basically the same as that of the example 1, and the difference is that the signal molecule producing bacteria mixed bacteria liquid is not added in the water inlet stage, and only the R2A liquid culture medium with the same volume is added.
After 10d incubation, example 1the reactor in the process has obvious aerobic granular sludge formation, the average grain diameter is 0.23mm, the complete granulation is realized after 15 days, the average grain diameter is 0.32mm, and SVI1044.6mL/g, and the 150 th day that the average grain diameter of the aerobic granular sludge is 2.8mm, the appearance contour is clear, and the sedimentation performance is good (SVI)10/SVI100.96 percent), high strength (the integrity factor is 99.1 percent), and COD and NH after complete granulation4 +、PO4 3-The average removal rates of (a) were 99.0%, 98.5% and 80.3%, respectively. In contrast, in the reactor of comparative example 1, fine particles did not appear until 28d, and complete granulation was not achieved at 45d, which was significantly longer than the time required for complete granulation in example 1, and at 150d, the average sludge particle size was 1.7mm, which was significantly smaller than that in example 1; SVI1052.4mL/g, an integrity coefficient of 97.5%, and COD and NH after complete granulation4 +、PO4 3-The average removal rates of (a) were 98.3%, 97.9% and 75.6%, respectively.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. The device disclosed by the embodiment corresponds to the method disclosed by the embodiment, so that the description is simple, and the relevant points can be referred to the method part for description.
the previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. The method for culturing the aerobic granular sludge is characterized by comprising the following steps of:
(1) Selecting mature aerobic granular sludge for ultrasonic crushing, and separating bacteria by adopting a dilution flat plate coating method;
(2) Sequentially numbering the single colonies with obvious morphological differences obtained by culturing in the step (1), selecting the single colonies, performing streak culture, and repeatedly executing the step for 3 times;
(3) Transferring the purified strain in the step (2) to a culture medium containing biological reporter bacteria, screening out signal molecule producing bacteria, re-screening for 2 times, and then carrying out 16s RNA sequencing comparison to identify the bacterial species;
(4) selecting 7 strains of bacteria from the identified strains for enrichment culture, mixing bacterial liquids in logarithmic phase in equal volume, determining the type of signal molecule generation, and using the mixed bacterial liquid of the signal molecule generating bacteria for biological enhancement;
(5) in the initial stage of culturing aerobic granular sludge in the SBR reactor, regularly and quantitatively adding a mixed bacterial liquid of signal molecule producing bacteria into the reactor, namely a biological strengthening process;
(6) stopping adding the signal molecule producing bacteria mixed bacterial liquid after the aerobic granular sludge is completely granulated;
(7) The SBR reactor is stably operated for 120-200 d.
2. The aerobic granular sludge culture method as claimed in claim 1, wherein the aerobic granular sludge matured in the step (1) is obtained from SBR reactor which is stably operated for more than 100 days and has the grain size range of 2-4mm and SVI1025-50mL/g，COD、NH4 +And PO4 3-the removal rates of (a) are greater than 90%, 90% and 70%, respectively.
3. The aerobic granular sludge culture method as claimed in claim 1, wherein the ultrasonication in the step (1) is carried out under the following conditions: the ultrasonic power is 100-200W, the ultrasonic frequency is 20-40kHz, and the ultrasonic time is 3-10 min.
4. The aerobic granular sludge culture method as claimed in claim 1, wherein the dilution plate coating method in step (1) is to apply the bacteria suspension after ultrasonic treatment to the R2A solid culture medium by gradient dilution with sterile Phosphate Buffered Saline (PBS) and culture at 25-30 ℃ for 12-36 h.
5. The aerobic granular sludge culture method as claimed in the claim 1, wherein the biological reporter bacterium in the step (3) is Agrobacterium tumefaciens KYC 55; the screening method of the signal molecule producing bacteria in the step (3) comprises the following steps: 5-20mL of overnight-cultured Agrobacterium tumefaciens KYC55 bacterial suspension, 5-20mL of AT buffer solution and 5-20mL of 1-3% water agar are fully mixed, 80-120 mu L of galactoside is added, the mixture is placed AT 40-50 ℃ for heat preservation, the mixed solution is poured into a culture dish, after the mixture is solidified, the strain to be detected is transferred onto a culture medium, the culture is carried out for 12-48h AT 20-30 ℃, the color change of the strain is observed, and the blue strain is the signal molecule generating strain.
6. The aerobic granular sludge culture method according to claim 1, wherein the 7 strains of bacteria selected in the step (4) are respectively: sphingomonas sp.SPQ-06, Caulobacter sp.SPQ-12, Caulobacter viroides strain, Microbacterium azadirachtae, Novosphingobium sp.SPQ-04, Sphingomonas sp.Z-03 and Caulobacter sp.SPQ-06, and the gene sequences thereof are uploaded to NCBI database to obtain Genbank serial numbers of MK602660, MK602661, MK602698, MK602699, MK602700, MK602701 and MK 602702.
7. The method for cultivating aerobic granular sludge as claimed in claim 6, wherein the signal molecule type generated by the ultra high performance liquid chromatography analysis of the 7 bacteria mixed bacterial liquid in the step (4) is C6-HSL、3OC8-HSL、C8-HSL and C10-HSL。
8. The aerobic granular sludge cultivation method as claimed in any one of claims 1 to 7, wherein the SBR reactor in step (5) is operated in the order of influent anaerobic aeration sedimentation drainage with 4 hours each, wherein the aeration time is 90 to 180min, the sedimentation time is 3 to 20min, the volume exchange ratio of the drainage phase is 40 to 60%, and the SRT is controlled to be 20 to 30 d.
9. The aerobic granular sludge culture method as claimed in any one of claims 1 to 7, wherein the inoculated sludge of the SBR reactor in the step (5) is flocculent activated sludge taken from an aeration tank or a sedimentation tank of a municipal sewage treatment plant, the MLSS at the initial stage of inoculation is 2 to 5g/L, and the SVI is3085-150mL/g, water inlet substrate is simulated town domestic sewage artificially configured in laboratory, wherein COD is 300-700mg/L, NH4 +Is 30-70mg/L, PO4 3-5-20 mg/L; the signal molecule producing bacterium liquid in the step (5) is added in a way that OD is added6000.5-1.5, 0.1-2g/L MLSS and (0.1-5) of simulated domestic sewage volume ratio: the proportion of 100 is added in the water inlet stage.
10. The aerobic granular sludge culture method as claimed in claim 1, wherein the complete granulation of the aerobic granular sludge in the step (6) means that the average sludge particle size is larger than 0.3mm, SVI30/SVI10The ratio is greater than 0.9.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111018103A (en) * | 2020-01-15 | 2020-04-17 | 黑龙江科技大学 | Method for directionally regulating and controlling stable operation of aerobic granular sludge |
| CN113184982A (en) * | 2021-04-19 | 2021-07-30 | 西安建筑科技大学 | Strengthening method and device for improving sedimentation performance of activated sludge |
| CN113233579A (en) * | 2021-04-19 | 2021-08-10 | 西安建筑科技大学 | Strengthening method and device for aerobic sludge granulation |
| CN113444661A (en) * | 2021-06-21 | 2021-09-28 | 温州大学 | Sphingobacterium neoformans and application thereof in wastewater dephosphorization |
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2019
- 2019-09-05 CN CN201910838030.2A patent/CN110563132A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111018103A (en) * | 2020-01-15 | 2020-04-17 | 黑龙江科技大学 | Method for directionally regulating and controlling stable operation of aerobic granular sludge |
| CN113184982A (en) * | 2021-04-19 | 2021-07-30 | 西安建筑科技大学 | Strengthening method and device for improving sedimentation performance of activated sludge |
| CN113233579A (en) * | 2021-04-19 | 2021-08-10 | 西安建筑科技大学 | Strengthening method and device for aerobic sludge granulation |
| CN113444661A (en) * | 2021-06-21 | 2021-09-28 | 温州大学 | Sphingobacterium neoformans and application thereof in wastewater dephosphorization |
| CN113444661B (en) * | 2021-06-21 | 2022-03-04 | 温州大学 | Sphingobacterium neoformans and application thereof in wastewater dephosphorization |
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