CN113184982A - Strengthening method and device for improving sedimentation performance of activated sludge - Google Patents
Strengthening method and device for improving sedimentation performance of activated sludge Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
- C02F3/12—Activated sludge processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biological Treatment Of Waste Water (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
The invention discloses a strengthening method and a strengthening device for improving the sedimentation performance of activated sludge, wherein the method comprises the following steps: carrying out immobilized embedding treatment on the pre-obtained aeromonas hydrophila by utilizing a polyvinyl alcohol embedding system to obtain the aeromonas hydrophila subjected to immobilized embedding treatment; the aeromonas hydrophila after the immobilized embedding treatment is put into the activated sludge to be treated, so that the sedimentation performance of the activated sludge is improved. The invention can solve one or more of the technical problems of poor activated sludge settling performance, difficult sludge-water separation and the like in the current sewage treatment process; the invention can increase the sludge particle size, shorten the sludge-water separation time, prolong the efficient and stable operation period, improve the effluent quality and save the cost.
Description
Technical Field
The invention belongs to the technical field of biological wastewater treatment, and particularly relates to a strengthening method and a strengthening device for improving the sedimentation performance of activated sludge.
Background
The most of the existing urban sewage treatment plants adopt an activated sludge method, and the technical problems of poor activated sludge settling performance, difficult sludge-water separation and the like in the daily operation process of the sewage treatment plants often cause troubles to the operation. Although the problems can be solved by adding nutrient salts and trace elements, adding coagulant, accelerating the sludge discharge or adjusting the aeration rate and other measures, the negative effects of high residual sludge yield, fluctuation of the effluent quality of the system, increase of the cost and the like can be generated at the same time.
In recent years, the application of microbial quorum sensing to biological sewage treatment has been attracting much attention, and studies have been made to improve the settling performance of sludge and to promote the granulation process of sludge by quorum sensing. At present, the methods are mostly realized by directly adding signal molecules, although the method of directly adding the signal molecules can strengthen the quorum sensing effect of microorganisms in the system in a short period, the cost is higher, the signal molecules easily lose functions along with the water loss of a reaction tank, and the strengthening effect cannot be sustained; in addition, the addition of high concentrations of signal molecules can disrupt the system equilibrium, thereby reducing the activity of bacteria, particularly nitrifying bacteria.
In conclusion, the development of a sustainable method or system for enhancing the microbiota effect has important significance in improving the sludge sedimentation performance and solving the problem of difficult sludge-water separation, and can provide effective technical support for the water treatment technology.
Disclosure of Invention
The invention aims to provide a strengthening method and a strengthening device for improving the sedimentation performance of activated sludge, and aims to solve one or more of the technical problems of poor sedimentation performance of activated sludge, difficult sludge-water separation and the like in the existing sewage treatment process. The invention can increase the sludge particle size, shorten the sludge-water separation time, prolong the efficient and stable operation period, improve the effluent quality and save the cost.
In order to achieve the purpose, the invention adopts the following technical scheme:
the invention relates to a strengthening method for improving the sedimentation performance of activated sludge, which comprises the following steps:
immobilizing and embedding the pre-obtained aeromonas hydrophila by using a polyvinyl alcohol embedding system, wherein the volume ratio of the carrier formed after immobilization to the muddy water mixed solution is 1:30, and obtaining the aeromonas hydrophila after immobilization and embedding;
the aeromonas hydrophila after the immobilized embedding treatment is put into the activated sludge to be treated, so that the sedimentation performance of the activated sludge is improved.
In a further improvement of the present invention, the pre-harvested AHLs signal molecules secreted by Aeromonas hydrophila comprise C4-HSL and C6-HSL signal molecules.
The invention has the further improvement that the step of immobilizing the pre-obtained aeromonas hydrophila by using a polyvinyl alcohol embedding system to obtain the immobilized aeromonas hydrophila specifically comprises the following steps:
culturing and centrifuging aeromonas hydrophila to obtain a bacterial suspension;
mixing the carrier solution with the bacterial suspension, adding CaCl2Uniformly stirring, circularly freezing for a plurality of times, and cutting into pieces to obtain the treated aeromonas hydrophila; wherein, polyvinyl alcohol and sodium alginate are added into the carrier solution.
The invention has the further improvement that in the carrier solution, the mass ratio of the polyvinyl alcohol to the sodium alginate is 10: 1.
the further improvement of the invention is that the step of putting the aeromonas hydrophila subjected to immobilized embedding treatment into the activated sludge to be treated to improve the sedimentation performance of the activated sludge specifically comprises the following steps:
putting activated sludge to be treated into a reaction kettle;
in the aeration stage, the aeromonas hydrophila subjected to the immobilized embedding treatment is placed into a reaction kettle, the aeromonas hydrophila subjected to the immobilized embedding treatment is fully mixed with the activated sludge to be treated, and the settling performance of the sludge is improved by utilizing C4-HSL and C6-HSL which are continuously secreted by the aeromonas hydrophila.
The invention relates to a strengthening device for improving the sedimentation performance of activated sludge, which comprises:
the immobilized embedding processing unit is used for carrying out immobilized embedding processing on the pre-obtained aeromonas hydrophila by utilizing a polyvinyl alcohol embedding system to obtain the aeromonas hydrophila after the immobilized embedding processing;
and the strengthening unit is used for putting the aeromonas hydrophila subjected to immobilized embedding treatment into the activated sludge to be treated so as to improve the sedimentation performance of the activated sludge.
In a further improvement of the present invention, the pre-harvested AHLs signal molecules secreted by Aeromonas hydrophila comprise C4-HSL and C6-HSL signal molecules.
The invention has the further improvement that the step of immobilizing the pre-obtained aeromonas hydrophila by using a polyvinyl alcohol embedding system to obtain the immobilized aeromonas hydrophila specifically comprises the following steps:
culturing and centrifuging aeromonas hydrophila to obtain a bacterial suspension;
mixing the carrier solution with the bacterial suspension, adding CaCl2Uniformly stirring, circularly freezing for a plurality of times, and cutting into pieces to obtain the treated aeromonas hydrophila; wherein, polyvinyl alcohol and sodium alginate are added into the carrier solution.
The invention has the further improvement that in the carrier solution, the mass ratio of the polyvinyl alcohol to the sodium alginate is 10: 1.
the further improvement of the invention is that the step of putting the aeromonas hydrophila subjected to immobilized embedding treatment into the activated sludge to be treated to improve the sedimentation performance of the activated sludge specifically comprises the following steps:
putting activated sludge to be treated into a reaction kettle;
in the aeration stage, the aeromonas hydrophila subjected to the immobilized embedding treatment is placed into a reaction kettle, the aeromonas hydrophila subjected to the immobilized embedding treatment is fully mixed with the activated sludge to be treated, and the settling performance of the sludge is improved by utilizing C4-HSL and C6-HSL which are continuously secreted by the aeromonas hydrophila;
the volume ratio of the carrier formed after immobilization to the sludge-water mixed liquid of the activated sludge is 1: 30.
Compared with the prior art, the invention has the following beneficial effects:
according to the method, the AHLs signal molecules secreted by the aeromonas hydrophila ATCC7966 are used for enhancing the induction of microorganisms in the system so as to improve the sedimentation performance of the activated sludge, shorten the sludge-water separation time, prolong the efficient and stable operation period, improve the effluent quality and save the cost.
In the invention, a polyvinyl alcohol embedding system is adopted to carry out immobilized embedding treatment on the strains and then the strains are put into a sewage treatment process for reinforcement, thereby avoiding a series of problems of signal molecule loss, system balance system damage, bacterial activity reduction and the like which are possibly caused by directly adding the strains. The outer dense and inner sparse porous structure of the polyvinyl alcohol embedding system and the excellent mechanical strength thereof ensure the bacterial quantity of the bacterial strain, can well and stably secrete C4-HSL and C6-HSL signal molecules in sewage under different conditions, and improve the sludge settling efficiency.
In the invention, the signal molecule secreting bacteria after embedding treatment can avoid the cost caused by continuous adding of the signal molecules. The process can be used for producing strains capable of secreting C4-HSL and C6-HSL, and can be used continuously with controllable cost. And the signal molecules of C4-HSL and C6-HSL can effectively strengthen the quorum sensing effect of microorganisms in the sewage, thereby improving the sedimentation performance of the sludge in the system. Thereby reducing the treatment pressure of the subsequent secondary sedimentation tank, even saving the secondary sedimentation tank and greatly reducing the capital construction, operation and maintenance costs of the sewage treatment plant. Meanwhile, the high activity, the preservability, the recoverability of the lost activity and the high-quality characteristic of almost no sludge generation in the operation of the polyvinyl alcohol embedding system further ensure the high utilization rate, the high recovery rate and the low pollution rate of the bacterial strain. And the low price is more beneficial to engineering application and practical popularization.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art are briefly introduced below; it is obvious that the drawings in the following description are some embodiments of the invention, and that for a person skilled in the art, other drawings can be derived from them without inventive effort.
FIG. 1 is a schematic flow diagram of an enhanced method for enhancing activated sludge settling performance according to an embodiment of the present invention;
FIG. 2 is a schematic view of an immobilized embedding system used in an example of the present invention;
FIG. 3 is a schematic view of an embodiment of an enhancement device in accordance with the present invention;
in the figure, 1, a water inlet pump; 2. a liquid level meter; 3. an electromagnetic valve; 4. an electric cabinet; 5. an air flow meter; 6. an aeration pump.
Detailed Description
In order to make the purpose, technical effect and technical solution of the embodiments of the present invention clearer, the following clearly and completely describes the technical solution of the embodiments of the present invention with reference to the drawings in the embodiments of the present invention; it is to be understood that the described embodiments are only some of the embodiments of the present invention. Other embodiments, which can be derived by one of ordinary skill in the art from the disclosed embodiments without inventive faculty, are intended to be within the scope of the invention.
Referring to fig. 1, an enhancing method for improving activated sludge settling performance according to an embodiment of the present invention includes the following steps:
aeromonas hydrophila (Aeromonas hydrophila ATCC7966) capable of secreting AHLs signal molecules was purchased from Baiohobowei Biotechnology Ltd, Beijing.
Immobilizing the aeromonas hydrophila ATCC7966 by using a polyvinyl alcohol embedding system to obtain treated aeromonas hydrophila ATCC 7966;
wherein said aeromonas hydrophila (aeromonas hydrophila atcc7966) is capable of secreting AHLs signal molecules; the treated aeromonas hydrophila (Aeromonas hydrophila ATCC7966) is used for improving the sedimentation performance of activated sludge.
The principle of the embodiment of the invention comprises the following steps: aeromonas hydrophila (Aeromonas hydrophila ATCC7966) which is purchased from Beijing Baiohobowei Wei Biotechnology Limited and can secrete AHLs signal molecules is detected and identified to be C4-HSL and C6-HSL respectively, the existence of the two signal molecules can strengthen the induction effect of microbial flora and promote the combination and aggregation of sludge microorganisms, thereby forming a microbial aggregate and improving the sedimentation performance. On the basis, polyvinyl alcohol (PVA) is adopted to carry out immobilized embedding on aeromonas hydrophila ATCC7966, so that biological loss caused by system drainage when a microbial inoculum is directly added and reduction of biological activity caused by microbial interspecific competition or environmental stress are avoided.
In the examples of the present invention, the Aeromonas hydrophila (Aeromonas hydrophila ATCC7966) was deposited under the accession number bio-05668; the addition of the strain with the preservation number of bio-05668 can increase the particle size of the activated sludge, make the sludge structure more compact, and obviously improve the sedimentation performance of the sludge, thereby shortening the sludge-water separation time in the sewage, and simultaneously improving the removal efficiency of the Chemical Oxygen Demand (COD), the Total Nitrogen (TN) and the Total Phosphorus (TP), thereby enhancing the treatment efficiency of the whole sewage treatment system.
Referring to fig. 1 to 3, an enhancing method for improving activated sludge settling performance according to an embodiment of the present invention includes the following steps:
(1) purchasing a strain: aeromonas hydrophila (Aeromonas hydrophila ATCC7966) capable of secreting AHLs signal molecules was purchased from Baiohobowei Biotechnology Ltd, Beijing.
(2) Identification of the signal molecule: the reporter strain CV026 can produce purplish rhzomorph, and signal molecules with side chain carbon chain length of C4-HSL to C8-HSL can be detected by using chromogenic reaction between purchased aeromonas hydrophila (Aeromonas hydrophylla ATCC7966) and the reporter strain.
(3) Determining a growth curve: and taking an LB culture medium without the added bacterial liquid as a blank control, measuring the absorbance at equal time intervals, continuously measuring for 48 hours and drawing a growth curve.
(4) Immobilization embedding: aeromonas hydrophila (Aeromonas hydrophila ATCC7966) is inoculated into 200mL LB culture medium, and concentrated bacterial liquid is obtained after culture and centrifugation. The volume ratio of the carrier solution added with polyvinyl alcohol (PVA) and Sodium Alginate (SA) with certain concentration to the bacterial suspension is 1:1 mixing and adding a certain amount of CaCl2Stirring, and cooling circularlyFreezing for several times, cutting into 4-5mm small pieces, and storing. Wherein the mass ratio of PVA to SA is 10: 1.
(5) and (3) putting the immobilized carrier into a reactor to improve the sludge settling property: in the aeration stage of the reactor, the immobilized aeromonas hydrophila (Aeromonas hydrophila ATCC7966) is added into the reaction system and fully mixed, and the settling performance of the sludge is improved by utilizing C4-HSL and C6-HSL which are continuously secreted by the aeromonas hydrophila (Aeromonas hydrophila ATCC7966) in the embedding system. Wherein the volume ratio of the carrier formed after immobilization to the sludge-water mixed liquid of the activated sludge is 1: 30.
The embodiment of the invention provides a biological strengthening method for immobilizing aeromonas hydrophila ATCC7966 (Aeromonas hydrophila ATCC7966) by utilizing a polyvinyl alcohol (PVA) embedding system to improve the sedimentation performance of sludge. The Aeromonas hydrophila (Aeromonas hydrophilus ATCC7966) was deposited under the accession number bio-05668.
In an embodiment of the invention, the specific steps of identifying the signal molecule comprise: aeromonas hydrophila (Aeromonas hydrophilus ATCC7966) capable of secreting AHLs signal molecules was purchased from Beijing Baiohobowenwei Biotechnology Ltd; the AHLs signal molecules secreted by the strain are subjected to high performance liquid phase-ion trap-time-of-flight mass spectrometry of Shimadzu corporation of Japan. Wherein 13 AHLs signal molecules with different molecular weights are selected as target compounds. The mobile phase A is ultrapure water containing 0.1% formic acid, the mobile phase B is chromatographic grade acetonitrile containing 0.1% formic acid, the chromatographic column used in the invention is a C18 column, the temperature of the chromatographic column is set to be 40 ℃, the flow rate of the mobile phase is 0.2mL/min, and the sample injection volume is 10 muL. The elution procedure for liquid chromatography was: stabilizing 10% B for 5min, increasing the concentration of the solvent B to 100% when 20min, maintaining for 2min, and acquiring data in a full scan mode to obtain a primary mass spectrogram of AHLs secreted by the strain A-L2. The reporter strain CV026 can produce purplish rhzomorph, and can detect signal molecules with side chain carbon chain lengths of C4-HSL to C8-HSL; a136 contains Ptral-lacZ fusion gene and expresses lacZ gene, when AHLs signal molecules of C4-HSL to C14-HSL or 3-oxo-C4-HSL to 3-oxo-C12-HSL exist in the environment, the reporter strain can generate color reaction, and the color is changed into green.
In the embodiment of the invention, the specific step of determining the growth curve comprises the following steps: (QS) measurement of growth curves of secretors: selecting a strain from the inclined plane in an aseptic operation platform, inoculating the strain into an LB liquid culture medium, and carrying out shake culture at the constant temperature of 37 ℃. Taking LB culture medium without adding bacteria liquid as blank control, measuring absorbance value of the culture liquid every 6h under the condition that the wavelength of a spectrophotometer is 600nm, and continuously sampling for 48 h. And drawing a growth curve of the dominant polycyclic aromatic hydrocarbon degrading bacteria by taking the culture time as an abscissa and the turbidity of the culture solution as an ordinate so as to observe the growth cycle of the bacteria.
Referring to fig. 2, the immobilization embedding step of the embodiment of the invention specifically includes: aeromonas hydrophila (Aeromonas hydrophila ATCC7966) A-L2 is inoculated into 200mL LB culture medium, cultured in a shaker at 37 ℃ and 150r/min for 18h, centrifuged at 8000rpm for ten minutes, the supernatant is decanted, washed with inorganic salt solution for three times, and resuspended to obtain concentrated bacterial liquid. Adding polyvinyl alcohol (PVA) and Sodium Alginate (SA) with a certain concentration into a beaker, adding sterile water, and heating and stirring in a water bath kettle at the temperature of more than 80 ℃ until the solution is in a uniform state. Mixing the carrier solution cooled to below 30 ℃ with the bacterial suspension at a volume ratio of 1:1, and adding a certain amount of CaCl2Stirring uniformly, and then pouring into a culture dish, wherein the thickness is about 4-5 mm. Freezing for 12h in a refrigerator at the temperature of minus 20 ℃ and 12h in a refrigerator at the temperature of 4 ℃, circulating for several times, cutting into small blocks of 4-5mm, cleaning with sterile water, and storing in a refrigerator at the temperature of 4 ℃ for later use. Wherein the mass ratio of PVA to SA is 10: 1.
in the embodiment of the invention, the carrier after immobilized embedding is put into a reactor to improve the sludge property: the polyvinyl alcohol (PVA) embedding system has a porous structure with dense outside and sparse inside and excellent mechanical strength, and in the aeration stage of the reactor, the aeromonas hydrophila after immobilization treatment is added into the reaction system and fully mixed, and the signal molecules C4-HSL and C6-HSL secreted by the aeromonas hydrophila in the embedding system can strengthen the quorum sensing effect and promote the combination and aggregation among sludge microorganisms, so that a microorganism aggregate is formed and the sedimentation performance is improved. In order to improve the fluidization characteristics and hydraulic characteristics of the immobilization carrier, a foaming agent is introduced to improve and adjust the specific gravity of the immobilization carrier to prevent the embedding carrier from sinking into water.
TABLE 1 sludge settling velocity and SVI in two sets of reactors during operation of the system30Comparison of
Table 1 shows the sludge settling rates and SVI in the two reactors during the operation of the system30SVI of the first 7 days, two groups30There was no significant difference. SVI of sludge in control group during sludge formation period30Gradually decreased and stabilized at 42.52mL/g, while the SVI of the group was enhanced30Stabilized at 28.45mL/g, which reflects that the sludge in the enhanced group system was dense compared to the control group, and also that there was no significant difference in the sludge settling rates of the two groups over the first 7 days. The sedimentation velocity of the sludge increases with the increase of the particle size with the increase of time, and reaches a maximum value at the 35 th day of culture, the control group is stabilized at 65.72m/h, and the reinforced group is stabilized at 80.12m/h, which shows that the inoculated strain can show good coagulation and sedimentation performance.
TABLE 2 removal efficiency of two sets of reaction systems for contaminants
As can be seen from Table 2, the COD concentration of the effluent of each reactor is reduced during the operation period and gradually kept stable after 7 days, the removal rates reach 95.43 percent and 96.43 percent respectively, and the addition of the bacterial strains plays an important role mainly. In 7 days, the TN removal rates of the control group and the strengthening group are 34.53% and 42.34% respectively, the TN removal rates of the control group and the strengthening group are stabilized at 82.31% and 87.31% respectively in 28 days, similarly, the total phosphorus removal rates of the two groups in the first 7 days are not obviously different, the total phosphorus removal rates are stabilized at 82.31% and 87.31% respectively in 28 days, and the TN quality of effluent reaches the first-class A standard. This indicates that the inoculated strain had no significant effect on the contaminant removal efficacy.
Referring to fig. 3, an enhancing apparatus for enhancing fast granulation of aerobic sludge according to an embodiment of the present invention includes: the device comprises a water inlet pump 1, a liquid level meter 2, an electromagnetic valve 3, an electric cabinet 4, an air flow meter 5, an aeration pump 6 and a sampling port;
wherein, strengthen granulation reaction unit and carry into in matrix to strengthening the granulation groove by intake pump 1, strengthen the bottom in granulation groove and connect aeration pump 6 and carry out the aeration, still even have air flow meter 5 to control aeration intensity and dissolved oxygen concentration between aeration pump 6 and tank bottom, the liquid level height of in-process of intaking is controlled to the inslot still has level gauge 2, whole device realizes the automated control of each part operation and supplies power through solenoid valve 3 through electric cabinet 4, gather the sample through the sample connection at last.
According to the invention, the added bacterial strain can increase the particle size of the activated sludge, so that the sludge structure is more compact, and the sedimentation performance of the sludge is obviously improved, thereby shortening the sludge-water separation time in the sewage, and simultaneously improving the removal efficiency of Chemical Oxygen Demand (COD), Total Nitrogen (TN) and Total Phosphorus (TP), so that the treatment efficiency of the whole sewage treatment system can be enhanced.
In the invention, a polyvinyl alcohol (PVA) embedding system is adopted to carry out immobilization embedding treatment on aeromonas hydrophila ATCC7966, and then the aeromonas hydrophila ATCC7966 is put into a sewage treatment process for reinforcement, thereby avoiding a series of problems of signal molecule loss, system balance system damage, bacterial activity reduction and the like which are possibly caused by directly adding the strain Aeromonas hydrophila ATCC 7966. The porous structure with dense outside and sparse inside of a polyvinyl alcohol (PVA) embedding system and the superior mechanical strength ensure that the bacterial load of Aeromonas hydrophila (Aeromonas hydrophila ATCC7966) is ensured, C4-HSL and C6-HSL signal molecules can be secreted well and stably in sewage under different conditions, and the sludge sedimentation efficiency is improved.
In the invention, aeromonas hydrophila (Aeromonas hydrophila ATCC7966) which is a signal molecule secreting bacterium after embedding treatment is adopted, so that the cost caused by continuous adding of the signal molecule can be avoided. The process can be used for continuously using aeromonas hydrophila (Aeromonas hydrophila ATCC7966) capable of secreting C4-HSL and C6-HSL, and has controllable cost. And the signal molecules of C4-HSL and C6-HSL can effectively strengthen the quorum sensing effect of microorganisms in the sewage, thereby improving the sedimentation performance of the sludge in the system. Thereby reducing the treatment pressure of the subsequent secondary sedimentation tank, even saving the secondary sedimentation tank and greatly reducing the capital construction, operation and maintenance costs of the sewage treatment plant. Meanwhile, the high activity, storability, activity loss recoverability and high-quality characteristic of almost no sludge generation in operation of the PVA embedding system further ensure the high utilization rate, high recovery rate and low pollution rate of aeromonas hydrophila (Aeromonas hydrophila ATCC 7966). And the low price is more beneficial to engineering application and practical popularization.
According to the technical scheme provided by the invention, Aeromonas hydrophila (Aeromonas hydrophila ATCC7966) which is purchased from Beijing Baiohobowenwei biotechnology limited and can secrete AHLs signal molecules is detected and analyzed, the signal molecules secreted by the strain are identified to be n-butyl homoserine lactone (C4-HSL) and nonyl homoserine lactone (C6-HSL), and the signal molecules are immobilized by utilizing a polyvinyl alcohol (PVA) embedding system. And the physicochemical property of the sludge in the activated sludge system and the content of signal molecules C4-HSL and C6-HSL are researched, and the addition of aeromonas hydrophila is proved to have obvious improvement effect on the sedimentation performance of the sludge.
Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art can make modifications and equivalents to the embodiments of the present invention without departing from the spirit and scope of the present invention, which is set forth in the claims of the present application.
Claims (10)
1. An enhancement method for improving the sedimentation performance of activated sludge is characterized by comprising the following steps:
carrying out immobilized embedding treatment on the pre-obtained aeromonas hydrophila by utilizing a polyvinyl alcohol embedding system to obtain the aeromonas hydrophila subjected to immobilized embedding treatment;
the aeromonas hydrophila after the immobilized embedding treatment is put into the activated sludge to be treated, so that the sedimentation performance of the activated sludge is improved.
2. The enhanced method for improving activated sludge settling performance of claim 1, wherein the pre-obtained aeromonas hydrophila secreted AHLs signal molecules comprise C4-HSL and C6-HSL signal molecules.
3. The method as claimed in claim 1, wherein the step of immobilizing the pre-obtained aeromonas hydrophila by using a polyvinyl alcohol embedding system to obtain immobilized aeromonas hydrophila comprises:
culturing and centrifuging aeromonas hydrophila to obtain a bacterial suspension;
mixing the carrier solution with the bacterial suspension, adding CaCl2Uniformly stirring, circularly freezing for a plurality of times, and cutting into pieces to obtain the treated aeromonas hydrophila; wherein, polyvinyl alcohol and sodium alginate are added into the carrier solution.
4. The strengthening method for improving the sedimentation performance of activated sludge according to claim 3, wherein the mass ratio of the polyvinyl alcohol to the sodium alginate in the carrier solution is 10: 1.
5. the strengthening method for improving the activated sludge settling property according to claim 1, wherein the step of putting the immobilized embedded aeromonas hydrophila into the activated sludge to be treated to improve the activated sludge settling property specifically comprises:
putting activated sludge to be treated into a reaction kettle;
in the aeration stage, the aeromonas hydrophila subjected to the immobilized embedding treatment is placed into a reaction kettle, the aeromonas hydrophila subjected to the immobilized embedding treatment is fully mixed with the activated sludge to be treated, and the settling performance of the sludge is improved by utilizing C4-HSL and C6-HSL which are continuously secreted by the aeromonas hydrophila;
the volume ratio of the carrier formed after immobilization to the sludge-water mixed liquid of the activated sludge is 1: 30.
6. An enhancement device for improving the sedimentation performance of activated sludge, comprising:
the immobilized embedding processing unit is used for carrying out immobilized embedding processing on the pre-obtained aeromonas hydrophila by utilizing a polyvinyl alcohol embedding system to obtain the aeromonas hydrophila after the immobilized embedding processing;
and the strengthening unit is used for putting the aeromonas hydrophila subjected to immobilized embedding treatment into the activated sludge to be treated so as to improve the sedimentation performance of the activated sludge.
7. The enhanced device for improving activated sludge settling performance of claim 6, wherein the pre-obtained aeromonas hydrophila secreted AHLs signal molecules comprise C4-HSL and C6-HSL signal molecules.
8. The strengthening device for improving the sedimentation performance of activated sludge according to claim 6, wherein the step of immobilizing the pre-obtained aeromonas hydrophila by using a polyvinyl alcohol embedding system to obtain the immobilized aeromonas hydrophila specifically comprises:
culturing and centrifuging aeromonas hydrophila to obtain a bacterial suspension;
mixing the carrier solution with the bacterial suspension, adding CaCl2Uniformly stirring, circularly freezing for a plurality of times, and cutting into pieces to obtain the treated aeromonas hydrophila; wherein, polyvinyl alcohol and sodium alginate are added into the carrier solution.
9. The enhancing apparatus for improving the sedimentation performance of activated sludge according to claim 8, wherein the carrier solution comprises polyvinyl alcohol and sodium alginate in a mass ratio of 10: 1.
10. the strengthening device for improving the activated sludge settling performance according to claim 6, wherein the step of putting the immobilized embedded aeromonas hydrophila into the activated sludge to be treated to improve the activated sludge settling performance specifically comprises:
putting activated sludge to be treated into a reaction kettle;
in the aeration stage, the aeromonas hydrophila subjected to the immobilized embedding treatment is placed into a reaction kettle, the aeromonas hydrophila subjected to the immobilized embedding treatment is fully mixed with the activated sludge to be treated, and the settling performance of the sludge is improved by utilizing C4-HSL and C6-HSL which are continuously secreted by the aeromonas hydrophila.
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| CN115820452A (en) * | 2022-07-05 | 2023-03-21 | 哈尔滨工业大学（深圳） | Aeromonas with autotrophic ammonia nitrogen assimilation capacity and application thereof |
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Application publication date: 20210730 |