CN1690190A - Process for preparing immobilization compound microorganism preparation for controlling secondary pollution of river channel bed mud - Google Patents
Process for preparing immobilization compound microorganism preparation for controlling secondary pollution of river channel bed mud Download PDFInfo
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- CN1690190A CN1690190A CN 200410026930 CN200410026930A CN1690190A CN 1690190 A CN1690190 A CN 1690190A CN 200410026930 CN200410026930 CN 200410026930 CN 200410026930 A CN200410026930 A CN 200410026930A CN 1690190 A CN1690190 A CN 1690190A
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Abstract
The invention relates to a method for preparation of immobilized complexion bacterial agent used as controlling the secondary pollution to river bottom mud, which is characterized in that individually prepare photosynthetic bacterium fermenting culture liquid and bacillus fermenting culture liquid, and prepare immobilized complexion bacterial agent in solid fermenting culture medium. The immobilized complexion bacterial agent is not easy to leak after putting into the polluted river, not only exerting the degradation of microbial bacterial agent to contaminant, and also exerting the adsorption and immobilization of zeolitic powder to the contaminant given off by bottom mud, and thus gaining the ends of decreasing the bottom mud pollution to superjacent water. The tests indicate that the pollution deactivation can decreases dramatically with the immobilized complexion bacterial agent developed in the invention.
Description
Technical field
The present invention relates to a kind of preparation method who is used to control the immobilization composite fungus agent of river bottom mud secondary pollution.
Background technology
Because long-term settlement, the accumulation of domestic pollutant cause the black smelly bed mud alluvial of city river, the city river sediment pollution is the black smelly important source of water body, and particularly after the pollution of water body external source was controlled, sediment pollution became the main source of water pollution.Contain large amount of organic matter, nutritive substance in the river bottom mud, the oxygen consumption rate height, generally be in strong reducing environment, the bed mud secondary pollution easily causes harmful substance contents such as ammonia nitrogen in the water body, nitrite, sulfide, skatole too high, cause water body " black smelly ", influence urban look and resident living.Therefore controlling the bed mud secondary pollution, is the important step of city river water surrounding improvement.The measure that in the past was used to control the bed mud secondary pollution mainly is to dredge to complete desilting, and this method cost is higher, and the remarketing of bed mud also is a complicated environmental protection difficult problem; By water body Loss-on-drying gas reoxygenation, can to a certain degree reduce sediment pollution, but more shallow river course is easily caused the bed mud resuspending; Add microbial preparation in the river course, this method has certain effect to strengthening water body self-purification ability, and shortcoming is that microbial preparation easily runs off in the river course, frequently adds, and cost is too high.
Summary of the invention
The object of the present invention is to provide a kind of preparation method who is used to control the immobilization composite bacteria of bed mud secondary pollution.
The microorganism that is used to produce immobilization composite bacteria of the present invention is the subtilis (Bacillussubtilis) of bacillus, and this bacterium is shaft-like, short chain, and rod end is semi-circular, the leather Lan Shi positive; And the Rhodopseudomonas palustris (Rhodpseudomonas palustris) of photosynthetic bacterium Rhodopseudomonas (Rhodpseudomonas sp), this bacterium is shaft-like, photoheterotrophic bacteria, leather Lan Shi feminine gender.The open in the literature record of above-mentioned two kinds of microorganisms (reference: 1. Ma Jianhua, Gao Yang, Niu Xiutian, etc. the purifying and the property research of the neutral 'beta '-mannase of subtilis. Chinese biological chemistry and molecular biosciences journal, 1999,15 (1): 79-82; 2. Cui Fu silk floss, Shi Jiaji, Lu Miaozhuan. the generation and the character of the neutral 'beta '-mannase of subtilis. microorganism journal, 1999,39 (1): 60-63; 3. the hole is built, Wang Wenxi, and Zhao Baige, etc. the research I. of subtilis B-903 bacterial strain is to the restraining effect and the control test of phytopathogen. Chinese biological control, 1999,15 (4): 157-161; 4. raise willow, Zhang Kexu, Liu Shuyun, etc. the research of subtilis D-ribose conditions of flask fermentation. Tianjin Light Industry College journal, 2001, (1): 11-13; 5. it is suitable to turn round and look at the ancestral, Qi Beijing, father-in-law's revive grain husk. the separation and the evaluation of Rhodopseudomonas palustris (Rhodopseudomonas palustris). and East China Normal University's journal (natural science edition), 1982, (4): 77-84; 6. Guo Qing literary composition, Ji Haiping. the research of Purple Non-sulfur photosynthetic bacterium. fodder industry, 1994,15 (3): 36-39; 7. wish state's qin, Jiang Jingying, Liu Wei, etc. separation and Culture of high reactivity photosynthetic bacterium and application. aquatic science, 1994,13 (1): 6-9.), wherein subtilis has been deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center, and deposit number is CGMCC No.0912, also has a series of bacillus subtilis strains to be deposited in Institute of Microorganism, Academia Sinica in addition, common micro-organisms culture presevation administrative center for example is numbered the bacterial strain of AS 1.398.Above-mentioned two kinds of microbial strains inventor also hold, and assurance can provide in 20 years that rise the present patent application day.
The present invention realizes in the following manner:
(1) prepares photosynthetic bacterium fermentation culture and fermentation of bacillus nutrient solution respectively
The preparation of photosynthetic bacterium fermentation culture:
Select Rhodopseudomonas palustris (Rhodpseudomonas palustris) bacterial classification for use, bacterial classification is inserted liquid nutrient medium, illuminance 1000～3000lx illumination cultivation under the anoxia condition, 25～35 ℃ of temperature, incubation time 48～72 hours obtains seed culture fluid; The inoculum size of pressing massfraction 5%～15% again inserts the liquid nutrient medium amplification culture, illuminance 1000～3000lx illumination cultivation under anoxia condition, and 25～35 ℃ of temperature, incubation time 72～96 hours obtains bacteria containing amount 10 * 10
8～20 * 10
8The photosynthetic bacterium fermentation culture of individual/milliliter; The prescription of described liquid nutrient medium is counted by massfraction: ammonium chloride (NH
4Cl) 0.10%～0.12%, dipotassium hydrogen phosphate (K
2HPO
4) 0.02%～0.03%, sodium-acetate (CH
3COONa) 0.40%～0.50%, sal epsom (MgSO
47H
2O) 0.05%～0.07%, sodium-chlor (NaCl) 0.40%～0.50%, calcium chloride (CaCl
2) 0.02%～0.04%, yeast extract paste 0.02%～0.04%, water 98.7%～98.99%;
The preparation of fermentation of bacillus nutrient solution:
Select for use subtilis (Bacillus subtilis) to be bacterial classification,, in the constant temperature shaking table, carry out aerobic cultivation the seed culture medium of bacterial classification access through sterilization, 30～35 ℃ of temperature, incubation time 24～48 hours obtains seed culture fluid; Press the fermention medium of the inoculum size access of massfraction 5%～15%, carry out aerobic cultivation through sterilization, 30～35 ℃ of temperature, incubation time 24～48 hours obtains bacteria containing amount 10 * 10
8～20 * 10
8The fermentation of bacillus nutrient solution of individual/milliliter; The prescription of described seed culture medium is counted by massfraction: yeast extract paste 0.10%～0.12%, glucose 1.0%～1.5%, Trisodium Citrate 0.05%～0.06%, ferrous sulfate (FeSO
47H
2O) 0.01%～0.02%, manganous sulfate (MnSO
4H
2O) 0.05%～0.06%, calcium chloride (CaCl
22H
2O) 0.01%～0.02%, water 98.22%～98.78%; The prescription of described fermention medium is counted by massfraction: yeast extract paste 0.10%～0.12%, molasses 2.0%～2.5%, Trisodium Citrate 0.05%～0.06%, ferrous sulfate (FeSO
47H
2O) 0.01%～0.02%, manganous sulfate (MnSO
4H
2O) 0.05%～0.06%, calcium chloride (CaCl
22H
2O) 0.01%～0.02%, water 97.22%～97.78%;
(2) preparation immobilization composite fungus agent:
Press massfraction 2%～3% photosynthetic bacterium fermentation culture, the inoculum size of massfraction 2%～3% fermentation of bacillus nutrient solution, access solid fermentation substratum is also mixed thoroughly, spreads out, and thickness is 5～10 centimetres, illumination cultivation is 120～144 hours under illuminance 200～1000lx, keep 25～35 ℃ of temperature in the culturing process, cultivation is dried under 60～70 ℃ of conditions after finishing, and pulverize, obtain total bacteria containing amount 5 * 10
8～10 * 10
8The composite fungus agent of individual/gram; The prescription of described solid fermentation substratum is counted by massfraction: bran powder 17%～19%, ammonium sulfate 0.5%～1.0%, calcium superphosphate 0.5%～1.0%; Potassium fulvate 0.5%～1.0%, lime carbonate 0.5%～1.0%, zeolite powder 37%～39%, water 38%～44%.
Immobilization composite fungus agent of the present invention is used to control the using method of river bottom mud secondary pollution:
With the even dried polluted river channel that spreads of immobilization composite fungus agent, and be deposited to the end during use, usage quantity is 30～90 gram/rice
2
Advantage of the present invention:
After immobilization composite fungus agent of the present invention drops into polluted river channel, be difficult for running off, not only bring into play the Degradation of microbiobacterial agent, also can bring into play the absorption fixed action of zeolite powder, reach and reduce the purpose that bed mud pollutes overlying water bed mud release pollutent to pollutent.Test shows, uses the immobilization composite fungus agent of developing among the present invention, can make bed mud that the pollution of overlying water is discharged obviously decline.
Embodiment
Only separate Rhodopseudomonas palustris (Rhodpseudomonas palustris) bacterial strain and subtilis (Bacillus subtilis) bacterial strain explanation embodiment of the present invention and the effect that obtains below, but that the present invention is not limited to is following with laboratory of the present invention
Embodiment.
Embodiment 1:
At first prepare the immobilization composite fungus agent, in the simulation river course, use the contrast experiment then.
The preparation method is as follows for the immobilization composite fungus agent:
With Rhodopseudomonas palustris (Rhodpseudomonas palustris) ZZH-01 bacterial strain is bacterial classification, and bacterial classification is inserted liquid nutrient medium, illuminance 1000lx illumination cultivation under the anoxia condition, and 30 ℃ of temperature, incubation time 72 hours obtains seed culture fluid; Insert the liquid nutrient medium amplification culture by 10% inoculum size again, illuminance 1000lx illumination cultivation under the anoxia condition, 30 ℃ of temperature, incubation time 96 hours obtains bacteria containing amount 20 * 10
8The photosynthetic bacterium fermentation culture of individual/milliliter.The prescription of photosynthetic bacterium liquid nutrient medium is counted by massfraction: ammonium chloride (NH
4Cl) 0.11%, dipotassium hydrogen phosphate (K
2HPO
4) 0.025%, sodium-acetate (CH
3COONa) 0.45%, sal epsom (MgSO
47H
2O) 0.06%, sodium-chlor (NaCl) 0.45%, calcium chloride (CaCl
2): 0.03%, yeast extract paste 0.03%, water 98.845%.
With subtilis (Bacillus subtilis) KC-01 bacterial strain is bacterial classification, with the seed culture medium of bacterial classification access through sterilization, carries out aerobic cultivation in the constant temperature shaking table, 35 ℃ of temperature, and incubation time 48 hours obtains seed culture fluid; Press the fermention medium of the inoculum size access of massfraction 10%, carry out aerobic cultivation through sterilization, 35 ℃ of temperature, incubation time 48 hours obtains bacteria containing amount 15 * 10
8The fermentation of bacillus nutrient solution of individual/milliliter.The prescription of genus bacillus seed culture medium is counted by massfraction: yeast extract paste 0.11%, glucose 1.20%, Trisodium Citrate 0.05%, ferrous sulfate (FeSO
47H
2O) 0.01%, manganous sulfate (MnSO
4H
2O) 0.05%, calcium chloride (CaCl
22H
2O) 0.01%, water 98.57%.The prescription of bacillus fermentation substratum by massfraction is: yeast extract paste 0.11%, molasses 2.2%, Trisodium Citrate 0.05%, ferrous sulfate (FeSO
47H
2O) 0.01%, manganous sulfate (MnSO
4H
2O) 0.05%, calcium chloride (CaCl
22H
2O) 0.01%, water 97.57%.
Press massfraction 2% photosynthetic bacterium fermentation culture, the inoculum size of massfraction 2% fermentation of bacillus nutrient solution, access solid fermentation substratum is also mixed thoroughly, spreads out on the cleaning cement flooring, and thickness is about 5 centimetres, illuminance 200lx illumination cultivation 120 hours, keep 30 ℃ of temperature, cultivation is dried under 70 ℃ of conditions after finishing, and pulverize, obtain total bacteria containing amount 9 * 10
8The immobilization composite fungus agent of individual/gram.The prescription of solid fermentation substratum by massfraction is: bran powder 18%, ammonium sulfate 0.5%, calcium superphosphate 1.0%, potassium fulvate 0.5%, lime carbonate 1.0%, zeolite powder 39%, water 40%.
The simulation river course is the aquarium reconstruction, and long 100 centimetres, wide 40 centimetres, high 50 meters, polluted bed mud is taken from contaminated inland river, Guangzhou and gushed, 5 centimetres of polluted bed mud thickness, and 120 liters of overlying water amounts (tap water) are furnished with water circulating pump, 5 liters/hour of circular flows.During experiment, establish an experimental group, another group is comparative group.Experimental group applies composite fungus agent 90 gram/rice
2, comparative group does not apply the immobilization composite fungus agent, through operation in 6 days, oxygen consumption organic COD in the comparative group overlying water
CrContent rises to 68 mg/litre, and ammonia nitrogen rises to 4.1 mg/litre, and sulfide rises to 0.09 mg/litre, and oxygen consumption organic COD in the experimental group overlying water
CrBe 31 mg/litre, ammonia nitrogen is 1.0 mg/litre, and sulfide is zero.Add composite fungus agent and can obviously reduce the pollution of bed mud overlying water.
Embodiment 2:
With Rhodopseudomonas palustris (Rhodpseudomonas palustris) ZZH-02 bacterial strain is bacterial classification, and bacterial classification is inserted liquid nutrient medium, illuminance 2000lx illumination cultivation under the anoxia condition, and 25 ℃ of temperature, incubation time 48 hours obtains seed culture fluid; The inoculum size of pressing massfraction 5% again inserts the liquid nutrient medium amplification culture, illuminance 1000lx illumination cultivation under the anoxia condition, and 25 ℃ of temperature, incubation time 72 hours obtains bacteria containing amount 10 * 10
8The photosynthetic bacterium fermentation culture of individual/milliliter.The prescription of photosynthetic bacterium liquid nutrient medium is counted by massfraction: ammonium chloride (NH
4Cl) 0.10%, dipotassium hydrogen phosphate (K
2HPO
4) 0.02%, sodium-acetate (CH
3COONa) 0.40%, sal epsom (MgSO
47H
2O) 0.05%, sodium-chlor (NaCl) 0.40%, calcium chloride (CaCl
2): 0.02%, yeast extract paste 0.02%, water 98.99%.
With subtilis (Bacillus subtilis) KC-02 bacterial strain is bacterial classification, with the seed culture medium of bacterial classification access through sterilization, carries out aerobic cultivation in the constant temperature shaking table, 30 ℃ of temperature, and incubation time 24 hours obtains seed culture fluid; Press the fermention medium of the inoculum size access of massfraction 5%, carry out aerobic cultivation through sterilization, 30 ℃ of temperature, incubation time 24 hours obtains bacteria containing amount 10 * 10
8The fermentation of bacillus nutrient solution of individual/milliliter.The prescription of genus bacillus seed culture medium is counted by massfraction: yeast extract paste 0.10%, glucose 1.0%, Trisodium Citrate 0.06%, ferrous sulfate (FeSO
47H
2O) 0.02%, manganous sulfate (MnSO
4H
2O) 0.06%, calcium chloride (CaCl
22H
2O) 0.02%, water 98.74%.The prescription of bacillus fermentation substratum is counted by massfraction: yeast extract paste 0.10%, molasses 2.0%, Trisodium Citrate 0.06%, ferrous sulfate (FeSO
47H
2O) 0.02%, manganous sulfate (MnSO
4H
2O) 0.06%, calcium chloride (CaCl
22H
2O) 0.02%, water 97.74%.
Press massfraction 3% photosynthetic bacterium fermentation culture, the inoculum size of massfraction 3% fermentation of bacillus nutrient solution, access solid fermentation substratum is also mixed thoroughly, spreads out on the cleaning cement flooring, and thickness is about 10 centimetres, illuminance 500lx illumination cultivation 120 hours, keep 25 ℃ of temperature, cultivation is dried under 60 ℃ of conditions after finishing, and pulverize, obtain total bacteria containing amount 5 * 10
8The immobilization composite fungus agent of individual/gram.The prescription of solid fermentation substratum is counted by massfraction: bran powder 17%, ammonium sulfate 1.0%, calcium superphosphate 0.5%, potassium fulvate 1.0%, lime carbonate 0.5%, zeolite powder 37%, water 43%.
Use contrast experiment in simulation in the river course as embodiment 1, institute's difference is to be 60 gram/rice with immobilization composite fungus agent dosage
2, through operation in 6 days, oxygen consumption organic COD in the comparative group overlying water
CrBe 68 mg/litre, ammonia nitrogen is 4.1 mg/litre, and sulfide is 0.09 mg/litre, and oxygen consumption organic COD in the experimental group overlying water
Cr39 mg/litre, ammonia nitrogen 1.1 mg/litre, sulfide is 0.01 mg/litre, adds the immobilization composite fungus agent control sediment pollution is had better effects.
Embodiment 3:
With Rhodopseudomonas palustris (Rhodpseudomonas palustris) ZZH-03 bacterial strain is bacterial classification, and bacterial classification is inserted liquid nutrient medium, illuminance 3000lx illumination cultivation under the anoxia condition, and 35 ℃ of temperature, incubation time 72 hours obtains seed culture fluid; Insert the liquid nutrient medium amplification culture by 15% inoculum size again, illuminance 3000lx illumination cultivation under the anoxia condition, 35 ℃ of temperature, incubation time 96 hours obtains bacteria containing amount 20 * 10
8The photosynthetic bacterium fermentation culture of individual/milliliter.The prescription of photosynthetic bacterium liquid nutrient medium is counted by massfraction: ammonium chloride (NH
4Cl) 0.12%, dipotassium hydrogen phosphate (K
2HPO
4) 0.03%, sodium-acetate (CH
3COONa) 0.5%, sal epsom (MgSO
47H
2O) 0.07%, sodium-chlor (NaCl) 0.5%, calcium chloride (CaCl
2): 0.04%, yeast extract paste 0.04%, water 98.7%.
With subtilis (Bacillus subtilis) KC-03 bacterial strain is bacterial classification, with the seed culture medium of bacterial classification access through sterilization, carries out aerobic cultivation in the constant temperature shaking table, 35 ℃ of temperature, and incubation time 48 hours obtains seed culture fluid; Press the fermention medium of the inoculum size access of massfraction 15%, carry out aerobic cultivation through sterilization, 35 ℃ of temperature, incubation time 48 hours obtains bacteria containing amount 20 * 10
8The fermentation of bacillus nutrient solution of individual/milliliter.The prescription of genus bacillus seed culture medium is counted by massfraction: yeast extract paste 0.12%, glucose 1.5%, Trisodium Citrate 0.05%, ferrous sulfate (FeSO
47H
2O) 0.01%, manganous sulfate (MnSO
4H
2O) 0.05%, calcium chloride (CaCl
22H
2O) 0.01%, water 98.26%.The prescription of bacillus fermentation substratum by massfraction is: yeast extract paste 0.12%, molasses 2.5%, Trisodium Citrate 0.05%, ferrous sulfate (FeSO
47H
2O) 0.01%, manganous sulfate (MnSO
4H
2O) 0.05%, calcium chloride (CaCl
22H
2O) 0.01%, water 97.26%.
Press massfraction 3% photosynthetic bacterium fermentation culture, the inoculum size of massfraction 3% fermentation of bacillus nutrient solution, access solid fermentation substratum is also mixed thoroughly, spreads out on the cleaning cement flooring, and thickness is about 10 centimetres, illuminance 1000lx illumination cultivation 144 hours, keep 35 ℃ of temperature, cultivation is dried under 70 ℃ of conditions after finishing, and pulverize, obtain total bacteria containing amount 10 * 10
8The immobilization composite fungus agent of individual/gram.The prescription of solid fermentation substratum is counted by massfraction: bran powder 19%, ammonium sulfate 0.5%, calcium superphosphate 1.0%, potassium fulvate 0.5%, lime carbonate 1.0%, zeolite powder 39%, water 39%.
Use the contrast experiment by embodiment 1 in the simulation river course, institute's difference is that immobilization composite fungus agent dosage is kept to 30 gram/rice
2, through operation in 6 days, oxygen consumption organic COD in the comparative group overlying water
CrBe 68 mg/litre, ammonia nitrogen is 4.1 mg/litre, and sulfide is 0.09 mg/litre, and oxygen consumption organic COD in the experimental group overlying water
Cr40 mg/litre, ammonia nitrogen 1.5 mg/litre, sulfide is zero, it is obvious to the effect of control sediment pollution to add the immobilization composite fungus agent.
Claims (1)
1. preparation method who is used to control the immobilization composite fungus agent of river bottom mud secondary pollution, its feature comprises:
(1) prepares photosynthetic bacterium fermentation culture and fermentation of bacillus nutrient solution respectively
The preparation of photosynthetic bacterium fermentation culture:
Select Rhodopseudomonas palustris (Rhodpseudomonas palustris) bacterial classification for use, bacterial classification is inserted liquid nutrient medium, illuminance 1000～3000lx illumination cultivation under the anoxia condition, temperature 25～352, incubation time 48～72 hours obtains seed culture fluid; The inoculum size of pressing massfraction 5%～15% again inserts the liquid nutrient medium amplification culture, illuminance 1000～3000lx illumination cultivation under anoxia condition, and 25～35 ℃ of temperature, incubation time 72～96 hours obtains bacteria containing amount 10 * 10
8～20 * 10
8The photosynthetic bacterium fermentation culture of individual/milliliter; The prescription of described liquid nutrient medium is counted by massfraction: ammonium chloride NH
4Cl 0.10%～0.12%, dipotassium hydrogen phosphate K
2HPO
40.02%～0.03%, sodium-acetate CH
3COONa 0.40%～0.50%, sal epsom MgSO
47H
2O0.05%～0.07%, sodium chloride nacl 0.40%～0.50%, calcium chloride CaCl
20.02%～0.04%, yeast extract paste 0.02%～0.04%, water 98.7%～98.99%;
The preparation of fermentation of bacillus nutrient solution:
Select for use subtilis (Bacillus subtilis) to be bacterial classification,, in the constant temperature shaking table, carry out aerobic cultivation the seed culture medium of bacterial classification access through sterilization, 30～35 ℃ of temperature, incubation time 24～48 hours obtains seed culture fluid; Press the fermention medium of the inoculum size access of massfraction 5%～15%, carry out aerobic cultivation through sterilization, 30～35 ℃ of temperature, incubation time 24～48 hours obtains bacteria containing amount 10 * 10
8～20 * 10
8The fermentation of bacillus nutrient solution of individual/milliliter; The prescription of described seed culture medium is counted by massfraction: yeast extract paste 0.10%～0.12%, glucose 1.0%～1.5%, Trisodium Citrate 0.05%～0.06%, ferrous sulfate FeSO
47H
2O 0.01%～0.02%, manganous sulfate MnSO
4H
2O 0.05%～0.06%, calcium chloride CaCl
22H
2O0.01%～0.02%, water 98.22%～98.78%; The prescription of described fermention medium is counted by massfraction: yeast extract paste 0.10%～0.12%, molasses 2.0%～2.5%, Trisodium Citrate 0.05%～0.06%, ferrous sulfate FeSO
47H
2O 0.01%～0.02%, manganous sulfate MnSO
4H
2O 0.05%～0.06%, calcium chloride CaCl
22H
2O 0.01%～0.02%, water 97.22%～97.78%;
(2) preparation immobilization composite fungus agent:
Press massfraction 2%～3% photosynthetic bacterium fermentation culture, the inoculum size of massfraction 2%～3% fermentation of bacillus nutrient solution, access solid fermentation substratum is also mixed thoroughly, spreads out, and thickness is 5～10 centimetres, illumination cultivation is 120～144 hours under illuminance 200～1000lx, keep 25～35 ℃ of temperature in the culturing process, cultivation is dried under 60～70 ℃ of conditions after finishing, and pulverize, obtain total bacteria containing amount 5 * 10
8～10 * 10
8The composite fungus agent of individual/gram; The prescription of described solid fermentation substratum is counted by massfraction: bran powder 17%～19%, ammonium sulfate 0.5%～1.0%, calcium superphosphate 0.5%～1.0%; Potassium fulvate 0.5%～1.0%, lime carbonate 0.5%～1.0%, zeolite powder 37%～39%, water 38%～44%.
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| CN100441679C (en) * | 2006-11-24 | 2008-12-10 | 西安利能科技有限责任公司 | Bacterium composite nutrient |
| CN101177680B (en) * | 2006-11-10 | 2010-09-29 | 中国科学院沈阳应用生态研究所 | Method for preparing mixed bacteria immobilized particle |
| CN101948186A (en) * | 2010-08-31 | 2011-01-19 | 周宗南 | Comprehensive treatment method for repairing in-situ biological diversity in rivers and lakes |
| CN101717154B (en) * | 2009-11-30 | 2011-02-16 | 沈阳大学 | Complex biological growth promoter used for recovering river bottom sediment |
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- 2004-04-21 CN CNB2004100269300A patent/CN100519736C/en not_active Expired - Fee Related
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| CN100441679C (en) * | 2006-11-24 | 2008-12-10 | 西安利能科技有限责任公司 | Bacterium composite nutrient |
| CN101717154B (en) * | 2009-11-30 | 2011-02-16 | 沈阳大学 | Complex biological growth promoter used for recovering river bottom sediment |
| CN101948186B (en) * | 2010-08-31 | 2011-11-23 | 周宗南 | Comprehensive treatment method for repairing in-situ biological diversity in rivers and lakes |
| CN101948186A (en) * | 2010-08-31 | 2011-01-19 | 周宗南 | Comprehensive treatment method for repairing in-situ biological diversity in rivers and lakes |
| CN102050523B (en) * | 2010-12-29 | 2012-07-04 | 赵亚勋 | Method for treatment and in-situ ecological restoration of black and stink riverway by using microorganism |
| CN102050523A (en) * | 2010-12-29 | 2011-05-11 | 赵亚勋 | Method for treatment and in-situ ecological restoration of black and stink riverway by using microorganism |
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| CN102250768B (en) * | 2011-06-01 | 2013-05-01 | 康源绿洲微生物技术（北京）有限公司 | Method for preparing enzyme and bacterium composite agent for treating sewage and sludge |
| CN102583770A (en) * | 2012-01-16 | 2012-07-18 | 湖南农业大学 | Bamboo charcoal-photosynthetic bacteria integrated municipal sanitary wastewater treating agent |
| CN103952361A (en) * | 2014-05-12 | 2014-07-30 | 广东石油化工学院 | Culture method of submerged photosynthetic bacteria |
| CN104293798A (en) * | 2014-09-19 | 2015-01-21 | 上海亘卓生物工程有限公司 | Microbial preparation for reducing production of bottom sludge cake and preparation method thereof |
| CN104326635A (en) * | 2014-11-24 | 2015-02-04 | 湖北中化东方肥料有限公司 | High-efficiency modifying agent of substrate of aquiculture pond and preparation method of modifying agent |
| CN104326635B (en) * | 2014-11-24 | 2017-01-11 | 湖北中化东方肥料有限公司 | High-efficiency modifying agent of substrate of aquiculture pond and preparation method of modifying agent |
| CN104828959A (en) * | 2015-05-28 | 2015-08-12 | 陕西科技大学 | Preparing method of compound microorganism flocculant and application method of compound microorganism flocculant |
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